Surveys of beet cyst nematode in England 1977–80

Surveys of sugar beet crops for beet cyst nematode ( Heterodera schachtii Schmidt) in seven selected areas of England have provided data on distribution of the pest and on some factors affecting its occurrence. These data were mapped by CAMGRID computer program.
Infestations occurred mostly on the East Anglian fenland (14% of fields infested) and in this area were more frequent nearer the sugar beet processing factory. In the remaining areas the few infested fields (5% or less) were generally scattered but a small concentration of infestation occurred in the West Midlands in an area of intensive horticulture with frequent brassica crops. The survey data did not support the view that beet cyst nematode infestations occur more frequently near access points to the crop. In the surveyed areas frequency of host crops appears to be more important than soil type in determining the likelihood of infestation.     

Prevalence of Plasmodiophora brassicae in a spring oilseed rape growing area in central Sweden and factors influencing soil infestation levels

During 1986–87 the presence of clubroot in soils sampled from 190 fields was assessed using a bioassay method, based on baiting the soils with Brassica campestris spp. pekinesis cv. Granaat. Clubroot was detected in 148 (72%) of the fields investigated and, on average, 49.2% of the plants were infected according to the bioassay. Subsequent testing of fields in 1990 and 1992 (54 and 81 fields, respectively) where no further Brassica crops had been grown indicated a significant decrease in the degree of infestation to 7.1% in 1992. Clay soils showed, on average, the highest degree of infestation, and high infestation was recorded for a wide range of pH values (5.2–6.6). The highest degree of infestation was recorded on fields where oilseeds were grown five times during the period 1965–85. The results presented show that, in a field with 100% infestation, the level of infestation declined to below the detection level after a period of 17.3 years. The half-life of the spore inoculum was determined to be 3.6 years. During the sensitivity testing of different Brassica species and cultivars, it was found that Chinese cabbage showed a low percentage of infestation in two soils, whilst some oilseed rape and spring oilseed turnip rape cultivars showed high degrees of infestation in these soils.     

性信息素诱捕防治棉红铃虫

1980～1981年在浙江海盐和上虞进行了性信息素诱捕防治棉铃虫的大田试验。面积分别为3500、3000亩。试验证明,每亩设置3～4只诱捕盆的大面积诱捕防治能明显降低田间红铃虫雌蛾的交配率(下降68.2～96.7%),棉株受害程度也减轻,与常规农药防治的对照相比,花害率下降42.4～78.9%,铃害率下降30.3～54.4%,同时还减少了用药次数,间接起到保护天敌、减少环境污染的作用。因此认为,性信息素诱捕法有可能作为棉红铃虫综合防治中的一项有效措施。     

The demarcation of Globodera rostochiensis and Globodera pallida infestations in fields for seed potatoes, using a Monte Carlo approach

A Monte Carlo approach was utilized to calculate the size of the demarcation unit, necessary to cover infestations with Globodera rostochiensis and Globodera pallida (PCN), detected with the new EU-sampling method. Results from 90 sampled fields were used to obtain frequency distributions of the central population density (CPD), the length and width gradients of infestation foci and the distance between 'primary' and 'secondary' foci within one field section in the direction of cultivation. With a simulation model written in R, a free software environment for statistical computing, the dimensions of the demarcation unit were calculated, based on the dimensions of the sampling unit, the position of the 'primary' infestation within the sampling unit and the size of the infestation focus. It is concluded that a demarcation of 90% of possible foci positions and dimension, analogous to the specified detection probability of the EU sampling method, provides sufficient security to demarcate any infestation. With the preferred sampling unit of 11 m width and 300 m length (1/3 ha sampled), used in The Netherlands, only 0.61% of the cyst numbers will be situated outside the demarcated unit. These cyst numbers compare to 0.17% of total amount of cysts in the EU infestation model: 1 focus with a CPD of 100 cysts kg⁻¹ and three foci with CPDs of 50 cysts kg⁻¹. The required demarcation area has a width of 36 m and a (maximum) length of 402 m and covers an unit of 1.5 ha. The methodology described can be applied to all proposed sampling methods and sampling unit dimensions.     

Predicting the risk of weed infestation in winter oilseed rape crops

Chemical weed control before crop and weed emergence is a systematic practice in winter oilseed rape crops in France. It would be profitable both for farmers and the environment to predict the level of weed infestation early on in the growing season and to control weeds only when necessary using post‐emergence weed control. The objective of this paper was to develop and evaluate simple models to predict weed biomass in oilseed rape crops. The model input variables were related to weed population characteristics and farmers’ practices. The models can be used to classify oilseed rape plots into two categories: plots with a level of weed infestation above a threshold or those with level of weed infestation below a threshold. A data set including 3 years of experiments, conducted across several regions in France, was used to estimate the parameters and to evaluate the models. High values of sensitivity and specificity were obtained when weed biomass was predicted as a function of sowing date, type of soil tillage, soil mineral nitrogen, crop density, weed density at emergence, and main characteristics of the most abundant weed species. Model performance strongly decreased when input variables related to the weed population were not taken into account. The best models correctly classified 90% of the plots with high weed infestation and 64% of the plots with low weed infestation.     

Impact of Parthenium hysterophorus on grazing land communities in north‐eastern Ethiopia

An investigation into the impact of Parthenium hysterophorus infestation was conducted in 2007 in the north‐eastern grazing lands of Ethiopia. Data on the above‐ground and seedbank species diversity were collected from five areas, each having sites with low, medium, or high levels of weed infestation. A total of 72 species was found in all areas. They were categorized into grass species (23), other species (48), or P. hysterophorus for ease of interpretation. A regression analysis showed a highly significant, but negative, relationship between the above‐ground species diversity and evenness with P. hysterophorus abundance. The mean cover abundance for the three infestation levels was 33.4% for P. hysterophorus, 41.0% for the grass species, and 26.5% for the other species. The most dominant grass species under all infestation levels were Cynodon dactylon, Urochloa panicoides, and Chloris gayana, while Andropogon abyssinicus and Eragrostis spp. were dominant under the low and medium infestation levels, respectively, and Hyparrhenia hirta was dominant under the low infestation level. Among the other species, Solanum nigrum was the most dominant under the low infestation level and Datura stramonium and Xanthium spp. were the most dominant under the medium and high infestation levels, respectively. The above‐ground dry biomass of P. hysterophorus increased between the low and high infestation levels, while that of the grass or other species reduced in the high, as compared to the low, infestation level. Although the grass species density decreased significantly with successive increases in the P. hysterophorus infestation level, no such trend could be seen for the other species. Within the soil seed bank, the viable seed density for the grass species, other species, and P. hysterophorus were 25.7, 5.8, and 68.5%, respectively. Similarly, the soil seed bank under the low‐, medium‐, and high‐infestation sites was dominated by P. hysterophorus, which contributed 25.1, 65.4, and 87.4% of the viable seed bank, respectively. Although the overall similarity between the above‐ground vegetation composition and the soil seed bank was low, it was similar at the low‐infested site. Thus, the invasion by P. hysterophorus was found to critically endanger the biodiversity of the grazing lands, particularly for the different grass and forbs species in the area. These changes might adversely affect not only future agriculture, but also food security, unless appropriate practises are developed and implemented for P. hysterophorus management.     

Evaluation of eradication measures against Anoplophora chinensis in early stage infestations in Europe

Eradication efforts are described at 5 sites where infestations of Anoplophora chinensis have been detected in Europe since 2003. The infestations were in France (declared eradicated in 2006), in Croatia, in Italy (Rome) and in the Netherlands (Westland and Boskoop). Each of these infestations was on or nearby a nursery or location where plants originating from Eastern Asia had (probably) been stocked or sold. Infested plants were all found within a distance of 30 m from the presumed source of infestation except in the Rome‐infestation where infested plants, in total 40, were found within 500 m of the presumed source of infestation. The history of plant imports and local observations at 3 sites (France, Croatia and Westland in the Netherlands) suggests that larvae arrived in imported plants and adults emerged and laid eggs on plants in the surroundings between 1 and 5 years before the infestation was detected. It was not possible to make such an estimate for the Boskoop infestation in the Netherlands and the Rome infestation in Italy.     

Plant yield response to artificial infestation of the false chinch bug,Nysius raphanus, confined on spring canola

A 2-year study was conducted to evaluate the relationship of plant yield responses to artificial infestations of the false chinch bug (FCB)Nysius raphanus (Howard) on spring canola in Colorado, USA. Yield losses were greater when infestation occurred at the early flowering stage (EFS) than at the early pod stage (EPS), in both 2001 and 2002. In the first trial in 2001, with infestations of 10, 20 and 40 FCB/head, the respective average yield losses over all cultivars were 43%, 68% and 69%, respectively, compared with the non-infested control at the EFS, but 11%, 26% and 23% at the EPS. In the second trial in 2001, with infestations of 10, 20 and 40 FCB/head, the respective average yield losses over all cultivars were 26%, 58% and 55% at the early EFS, but 35%, 20% and 35% at the EPS. Yield reductions from FCB infestation were lower in 2002 than in 2001. In the first trial in 2002, with infestations of 10, 20 and 40 FCB/head, the respective average yield losses of all cultivars combined were 31%, 51% and 68% at the EFS, and 13%, 32% and 18% at the EPS. However, in the second trial in 2002, with the same numbers of FCB per head, no yield reductions were observed at either EFS or EPS. The number of FCB causing 10% yield loss in the four trials ranged from 6.1 to 39.4 FCB/head (avg. 14.8) following infestation at the EFS and 15.4–109.8 (avg. 41.8) following infestation at the EPS. Cultivar responses to FCB may also influence FCB yield reductions. However, in these studies all eight tested cultivars sustained yield loss in at least one trial at some FCB infestation level. Variation between trials was substantial but a significant level of resistance to FCB injury did not occur among the tested cultivars. http://www.phytoparasitica.org posting Sept. 18, 2006.     

Biotechnische Regulierung der Kohlmottenschildlaus in Kohlgem��se �C Sachstand und Perspektiven

The cabbage whitefly,Aleyrodes proletella, has developed to a key pest in brassica vegetable crops throughout Germany. In a 3-year project we investigated a biotechnical approach employing netting (0.8?×?0.8 mm meshsize) in combination with the native parasitoidEncarsia tricolor in an inoculative-inundative approach in organic Brussels sprouts under field conditions. In small scale plot experiments continuous netting from transplanting in May till October alone reduced whitefly larval densities by 77 % at peak infestation in all years. Remaining whitefly infestation under net was associated with the necessity of temporary net removal for the purpose of mechanical weeding, which caused primary whitefly infestation. Release of mass rearedE. tricolour under net at the first signs of larval infestation significantly increasedA. proletella-parasitation and reduced infestation compared to netted controls without release. Furthermore, at highest dosage in 2009,E. tricolor had a significant enhancing effect on raw yield and the quality level of marketable yield. Additional on-farm experiments without netting in 2008 and 2009 confirmed the control potential ofE. tricolor. Although the most important project mile stones have been achieved, namely (a)E. tricolour-establishment under net and in the open field, (b) enhanced parasitation corresponding with pest density decline and (c) a positive impact on yield and quality parameters, further research will be necessary. The focus should be on the initial infestation period, optimizing the timing, frequency and required dosage.     

Allelochemical resistance traits of muskmelon (Cucumis melo) against the fruit fly (Bactrocera cucurbitae) in a hot arid region of India

Host plant resistance is an important component for management of the melon fruit fly, Bactrocera cucurbitae (Coquillett), owing to difficulties associated with its chemical and biological control. Various biochemical traits including total sugar, reducing sugar, non-reducing sugar, tannins, phenols, alkaloids, flavinoid and pH contents of fruit were studied on 11varieties/ genotypes of muskmelon, Cucumis melo L., in relation to resistance against B. cucurbitae under field conditions. Significant differences were found in tested varieties/ genotypes for fruit infestation and larval density per fruit. AHMM/BR-1, RM-50 and AHMM/BR-8 were the most resistant; MHY-5, Durgapura Madhu and Pusa Sarabati were moderately resistant; AHMM/BR-13, Pusa Madhuras and Arka Jeet were susceptible; whereas Arka Rajhans and GMM-3 were the highly susceptible varieties/ genotypes to fruit fly in both seasons, 2011 and 2012. The larval density per fruit increased with an increase in percent fruit infestation and there was a significant positive correlation (r = 0.97) between percent fruit infestation and larval density per fruit. Total sugar, reducing sugar, non-reducing sugar and pH were lowest in resistant and highest in susceptible varieties/ genotypes, whereas tannins, phenols, alkaloids and flavinoid contents were highest in resistant and lowest in susceptible varieties/ genotypes. Total alkaloid and pH contents explained 97.96% of the total variation in fruit fly infestation and 92.83% of the total variation in larval density per fruit due to alkaloids and total sugar contents.     

Standorteigenschaften und Anbaumanagement erklären regionale Unterschiede im Kohlherniebefall in Deutschland

In the years 2013 and 2014 the presence and the infestation level of Plasmodiophora brassicae in six federal states of Germany were assessed using a bioassay method. Soils were sampled from 237 oilseed rape fields and the farmers provided information about the soil type, the soil pH values and the last year of oilseed rape cultivation. Clubroot was detected in 66 % of the fields investigated with a mean disease severity (BI) of 0.34. Between and within the federal states differences in presence and infestation level of P. brassicae were detected. In Schleswig-Holstein significantly more fields were infected by P. brassicae (90 %) and BI was relatively high (0.49) compared to Lower Saxony, Mecklenburg-Western Pomerania and North Rhine-Westphalia with 50 % infected fields and a mean BI of 0.27. Highly infested fields (BI > 0.8) were prevalent on 15 and 25 % of fields investigated in every federal state. Within the federal states the soil climate regions (BKR) differed significantly from each other. In BKR with light and sandy soils P. brassicae was less widespread compared to neighboring BKR with loamy soils. In addition, BKR with high infestation levels on fields investigated revealed a high presence of clubroot infested fields within this BKR. The infestation level in our study was significantly influenced by soil type, content of sand and soil pH value: soil types with a sand content of 30–35 % and a pH values lower than 5.6 showed high clubroot severity and disease incidences.     

Changes in the incidence of ragwort (Senecio jacobaea L.) infestation of grassland over a 4-year period in NE Scotland

A roadside survey of 4749 fields in eight areas of NE Scotland was conducted annually from 1979 to 1982. The proportion of grass fields infested with ragwort remained steady at 18–19% from 1979 to 1981 but fell to 13·5% in 1982. This fall resulted from fewer fields becoming newly infested and from more fields that had remained in grass ceasing to be infested. The proportion of infested fields remaining in grass that showed a reduction of two or more points on a 0–5 logarithmic scale of ragwort density (a possible indication of the frequency of control measures) was 24·0% in 1979–1980, 21·7% in 1980–1981 and 36·3% in 1981–1982. The incidence of ragwort infestation was consistently higher in some areas than in others and was not related to the proportion of arable crops. The change in incidence of infestation varied greatly between different areas, as did the proportion of infested fields whose score on the 0–5 scale fell by two or more points. In 1979–1980 and 1980–1981 this proportion had to exceed 22% to effect a net decrease in the incidence of infestation in an area, but in 1981–1982 no relationship was evident between these two measures of change. The incidence of infestation in all areas increased with increasing sward age but one area (Moray and Nairn) had a much higher proportion of young grass infested than any of the others. Over 99% of ragwort infestations consisted of Senecio jacobaea, infestations of S. aquatints Huds. being rare.     

转cry1Ab/cry2Aj、cry1Ab/vip3DA玉米对棉铃虫、甜菜夜蛾和斜纹夜蛾的抗虫性评价

为评估转cry1Ab/cry2Aj、cry1Ab/vip3DA玉米对棉铃虫Helicoverpa armigera(Hübner)、甜菜夜蛾Spodoptera exigua(Hübner)和斜纹夜蛾Prodenia litura(Fabricius)的抗虫性,在室内测定了3个转cry1Ab/cry2Aj玉米品系和1个转cry1Ab/vip3DA玉米品系对3种害虫幼虫存活和生长发育的影响,研究了该系列Bt玉米不同组织器官对害虫的杀虫活性和控制效果。结果显示,棉铃虫初孵幼虫取食各品系Bt玉米叶片96h后死亡率为87.50%~90.00%,取食花丝和雌穗的幼虫96h后几乎全部死亡;甜菜夜蛾初孵幼虫取食各品系Bt玉米叶片、花丝和雌穗168h后死亡率为22.50%~68.33%,幼虫的生长发育受到明显抑制,体重抑制率达85.00%~95.00%;斜纹夜蛾初孵幼虫取食各品系Bt玉米叶片、花丝和雌穗96 h后死亡率显著高于非转基因亲本对照,168h后幼虫死亡率达90.00%以上。研究表明,转cry1Ab/cry2Aj和cry1Ab/vip3DA玉米品系对棉铃虫和斜纹夜蛾的初孵幼虫表现出较好的抗性,可以作为转多基因抗虫玉米育种的备选材料。     

褐飞虱与白背飞虱为害诱导水稻防御反应的比较

为探究2种稻飞虱——褐飞虱Nilaparvata lugens(St?l)和白背飞虱Sogatella furcifera(Horváth)诱导的水稻防御反应差异,于室内测定了水稻在分别受褐飞虱或白背飞虱产卵雌成虫为害时,其茉莉酸、水杨酸、乙烯、H_2O_2以及挥发物含量的变化。结果表明,尽管褐飞虱和白背飞虱产卵雌成虫的为害均可以诱导水稻茉莉酸、水杨酸、乙烯和H_2O_2等防御相关信号分子以及一些水稻挥发物含量的增加,但是二者的诱导作用存在差异。水稻在受白背飞虱产卵雌成虫为害时,茉莉酸的含量在3 h时就显著升高,12 h时含量达到最高;而受褐飞虱产卵雌成虫为害时,6 h时茉莉酸含量才显著升高,72 h时含量达最高;并且在2种稻飞虱为害的3～48 h内,白背飞虱为害诱导的茉莉酸含量始终显著高于褐飞虱为害诱导的。水稻受白背飞虱产卵雌成虫为害24 h后诱导的水杨酸含量、为害48 h后诱导的乙烯含量、为害72 h后诱导的H_2O_2含量及为害24 h后诱导的挥发物释放量分别是褐飞虱产卵雌成虫为害诱导的1.28、1.45、4.10和1.77倍。表明水稻能识别褐飞虱和白背飞虱的为害,从而做出针对害虫种类特异性的防御反应;并且水稻对白背飞虱产卵雌成虫为害所做出的防御反应比对褐飞虱的更强烈。     

Spread of proso millet (Panicum miliaceum L.) in Ontario, Canada. I. Rate of spread and crop susceptibility

The infestations of two biotypes of Panicum miliaceum L. were monitored over a 5-year period (1981–1985) as they occurred in two townships in southwestern Ontario, Canada. On average, 16·5% of the cultivated fields in Hullett township were infested with the black-seeded biotype while 12·9% of the fields in North Norwich township were infested with the golden-seeded biotype. Neither population showed clear signs of increasing. The two biotypes were similar in their ability to persist as adult infestations. The black-seeded biotype showed a greater potential for colonizing uninfested fields. The crops grown in this region showed different susceptibilities to infestation by P. miliaceum. Corn (maize) fields were susceptible while most grain fields were resistant to infestation. White beans were susceptible to infestation by the black-seeded biotype. Wheat and oat crops were more resistant to infestation by the golden-seeded biotype than to those of the black-seeded biotype.     

Artenspektrum und Befallshäufigkeit von Fusarium spp. in Bt- und konventionellem Mais im Maiszünsler-Befallsgebiet Oderbruch

The European corn borer (Ostrinia nubilalis) is one of the most important pests of maize (Zea mays). Injuries to the plants caused by the larvae of the European corn borer may represent entrance gates for fungal-spores. The cultivation of Bacillus thuringiensis maize (Bt-maize) is one possibility to reduce infestation by the European corn borer. The aim of the present project was to determine and to compare the number of species and the frequency of Fusarium spp. infestation in Bt-maize (cry1Ab) and conventional maize. In 2003, we analysed the Fusarium spp. infestation of samples of chaffed Bt-maize and its isogenic variety on two experimental fields in the Oderbruch region (Germany), an European corn borer infested area. The conventional variety on the first of the experimental fields (previous crop wheat and forking cultivation) showed a small infestation (16%) of Ostrinia nubilalis while in the conventional variety on the second field (previous crop maize and not forking cultivation) the infestation of the European corn borer was almost three times higher (47%). In the conventional variety on both of the experimental fields we found a high Fusarium spp. infestation (70%). Especially species of the section Liseola dominates, among them: F. subglutinans, F. proliferatum und F. verticillioides. The Fusarium infestation in the samples of Bt-maize from the field with previous crop wheat and forking cultivation was just as high as in the conventional variety (70%). The infestation of Fusarium spp. in the samples of Bt-maize from the field with previous crop maize and forking cultivation was more than 20?% lower.     

Fall armyworm [Spodoptera frugiperda (J.E. Smith)] damage in maize: management options for flood-recession cropping smallholder farmers

Abstract

We determined fall armyworm abundance, infestation and damage on maize in smallholder farmers’ fields in the Zambezi Valley in northern Zimbabwe. Data were collected through field measurements at four sites and interviews with 101 farmers. Fall armyworm abundance ranged from 13.7 to 33.3 larvae per 30 plants, with infestation exceeding 94% and leaf, silk and tassel damage levels ranging between 25 and 50%. Most larvae on maize plants (P?<?0.05) were at instar stages 2 to 3. Estimated grain yield decrease was 58%. Echinochloa colona and Amaranthus spp. were also damaged by fall armyworm. Farmers experimented with 28 combinations of pesticides, ash, and washing powders. Sixty five percent of the farmers used restricted to highly restricted pesticides. High fall armyworm abundance, infestation and maize damage levels suggest that the Zambezi Valley could become a fall armyworm hotspot. Sequential damage of plant parts signified the persistence of this pest in the study area. We recommend large-scale interventions that include chemical and biological control methods as well as destroying ratoon hosts, publishing information about locally available pesticides for the control of fall armyworm, and farmer training on fall armyworm biology and correct use of pesticides.     

Assessment of the high-dose concept and level of control provided by MON 87701 × MON 89788 soybean against Anticarsia gemmatalis and Pseudoplusia includens (Lepidoptera: Noctuidae) in Brazil

BACKGROUND: Genetically modified MON 87701 × MON 89788 soybean (Glycine max), which expresses the Cry1Ac and EPSP‐synthase proteins, has been registered for commercial use in Brazil. To develop an Insect Resistance Management (IRM) program for this event, laboratory and field studies were conducted to assess the high‐dose concept and level of control it provides against Anticarsia gemmatalis and Pseudoplusia includens. RESULTS: The purified Cry1Ac protein was more active against A. gemmatalis [LC₅₀ (FL 95%) = 0.23 (0.15–0.34) µg Cry1Ac mL^?1 diet] than P. includens [LC₅₀ (FL 95%) = 3.72 (2.65–4.86) µg Cry1Ac mL^?1 diet]. In bioassays with freeze‐dried MON 87701 × MON 89788 soybean tissue diluted 25 times in an artificial diet, there was 100% mortality of A. gemmatalis and up to 95.79% mortality for P. includens. In leaf‐disc bioassays and under conditions of high artificial infestation in the greenhouse and natural infestation in the field, MON 87701 × MON 89788 soybean showed a high level of efficacy against both target pests. CONCLUSIONS: The MON 87701 × MON 89788 soybean provides a high level of control against A. gemmatalis and P. includes, but a high‐dose event only to A. gemmatalis. Copyright © 2012 Society of Chemical Industry     

Laboratory evaluation and field application of bacterial and fungal insecticides on the citrus flower moth,Prays citri miller (Lep., hyponomeutidae) in lime orchards in Egypt

The efficacy of two commercial products of Bactospeine and Bio-fly onPrays citri of lime trees was studied under laboratory and field conditions throughout the two seasons of 1996 and 1997. Laboratory results indicated that the pathogenic effect of Bactospeine,Bacillus thuringiensis (Berliner) was higher than that of Bio-fly,Beauveria bassiana in spite of the latter was applied with higher doses. Bactospeine at the rate (2 cc/1 l.w.) effected on the larvae more than on the adults, that treatment reduced no. of eggs/female by 81.70% and decreased the larval infestation by 90.08%. Bio-fly at the highest concentration (4 cc/1 l.w.) reduced the infestation with the eggs, the larvae and the pupae by 65.83%, 68.45% and 72.55%, respectively. Advanced effects of bacterial and fungal treatments on the final reduction rates ofP. citri infestation were recorded. Field applications revealed that two sprays with Bactospeine or Bio-fly gave good control ofP. citri more than one spray. Bactospeine at the rate (2 cc/1 l.w.) and Biofly at the rate (4 cc/1 l.w.) reduced the infestation by (89.82–93.43%) and (76.32–78.83%), respectively. In all cases, higher doses of bioinsecticides produced potentiation effects of infestation reduction. It is recommended that two sprays of Bactospeine at a rate (2cc/1 l.w.) are adequate effective to reduce the population of P. citri in lime orchards during the main flowering period of trees without need of chemical insecticides. An erratum to this article is available at .