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《中国畜牧兽医文摘》2016,(10)
小反刍兽疫作为外来动物疫病,于2014年初在娄底市自首次发生,给娄底市的养羊业造成了较大损失。2016年农业部制定了《全国小反刍兽疫消灭计划(2016-2020年)》,为各地消灭小反刍兽疫提供了科学指导。笔者结合该计划,就娄底市小反刍兽疫的防控工作开展情况及存在的问题进行了总结分析。 相似文献
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2015年农业部制订了全国小反刍兽疫消灭计划,提出到2020年力争全国达到小反刍兽疫非免疫无疫区标准.江苏为养羊大省,同时为小反刍兽疫高风险区,本文对江苏实施小反刍兽疫消灭计划的关键环节和主要困扰进行了分析,并提出了相关保障措施. 相似文献
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以隐孢子虫(Cryptosporidium spp.)18S rRNA为靶基因,通过巢氏PCR检测发现在采集的101份新鲜粪便样品中18份样本为阳性。不同地区羊场的隐孢子虫感染率分别为:李集镇36%(18/50),新安镇0%(0/30)、堆沟港镇0%(0/21)未检出隐孢子虫。但通过对4羊场的分析发现,有2个羊场(50%)为隐孢子虫感染阳性,且不同的羊场感染率差异显著,因此单纯的以地区来评价隐孢子虫的感染率,是值得商榷的。山羊隐孢子虫的感染率为33.3%,湖羊隐孢子虫的感染率为2%。2~6月龄的育肥羊隐孢子虫的感染率为36%,6~10月龄的育成羊(0%)。对检测为阳性的样品进行了隐孢子虫18S rRNA基因片段序列分析,发现18个样品全部为肖氏隐孢子虫(Cryptosporidium xiaoi),不存在泛在隐孢子虫(Cryptosporidium ubiquitum)。在检测隐孢子虫感染阳性的1个山羊场和1个羊湖羊场均存在肖氏隐孢子虫感染,不存在泛在隐孢子虫,更未发现肖氏隐孢子虫和泛在隐孢子虫的混合感染。目前的数据提示肖氏隐孢子虫对2~6月龄山羊(33.3%)和湖羊(2%)具有更高的... 相似文献
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为掌握青海省海北州藏羊群中牛病毒性腹泻病毒和羊边界病毒的感染情况,本研究采用RT-PCR方法分别对青海省海北州的161份健康藏羊血清样品和34份腹泻藏羊组织样品进行了BVDV和BDV的抗原核酸检测。结果显示:195份样品中BVDV和BDV总阳性率分别为29.74 %和14.36 %;161份健康藏羊血清样品中BVDV和BDV平均阳性率分别为26.71 %和11.80 %,BVDV/BDV混合感染率为4.35 %;34份腹泻藏羊组织样品中BVDV和BDV平均阳性率分别为44.12 %和26.47 %,BVDV/BDV混合感染率为17.65 %。本研究表明青海省海北州健康藏羊群和腹泻藏羊群中均存在BVDV、BDV的单独感染以及混合感染,且感染情况在个别养殖场(户)较为严重,本研究为青海藏羊的综合防控措施提供了指导依据,丰富了我国羊群中BVDV和BDV的流行病学资料。 相似文献
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K. S. Intisar Y. H. Ali M. A. Haj M. A. T. Sahar M. M. Shaza A. M. Baraa O. M. Ishag Y. M. Nouri K. M. Taha E. M. Nada A. M. Ahmed A. I. Khalafalla G. Libeau A. Diallo 《Tropical animal health and production》2017,49(4):747-754
The existence of peste des petits ruminants (PPR) in domestic ruminants and camels in Sudan during 2008–2012 was investigated. Lung tissues and serum samples were randomly collected from sheep, goats, cattle, and camels at different areas of Sudan. A total of 12,384 serum samples were collected from clinically healthy 7413 sheep, 1988 camels, 1501 cattle, 1459 goats, and 23 gazelles at different areas in the Sudan. They were examined for PPR antibodies using competitive ELISA (cELISA). The overall detected seroprevalence of PPR in tested sera was 49.4%; seroprevalence values within species were 67.1, 48.2, 25.8, 2.1, and 21.7% in sheep, goat, cattle, camels, and gazelles, respectively. The highest seroprevalence (68.1%) was observed in sera collected from Darfur states, then the central states (54.3%). A total of 1276 lung tissue samples (623 sheep, 324 cattle, 220 camels, and 109 goats) were collected. The majority of lung samples were collected from clinically healthy animals that showed lesions on PM in slaughterhouses (95%) and during PPR outbreaks; samples were tested for PPR antigen using immunocapture ELISA (IcELISA). PPR antigen was detected in 233 out of the 1276 tested samples (18.3%). Positive results were observed in samples collected from clinically healthy and diseased animals. The observed prevalence values in each species were 33.6, 21.1, 15.4, and 12.3% in camel, goat, sheep, and cattle, respectively. PPR antigen was detected in samples from different areas; however, the highest prevalence (63.9%) was found in samples collected from the eastern states, then Khartoum state (28%). Trials for virus isolation were done in different cell cultures. Out of 30 IcELISA-positive samples inoculated in primary bovine and ovine kidney cells, Vero cells, the PPR virus was successfully isolated from 15 (eight sheep, five camels, and two goats) samples in the three cell culture types. Using RT-PCR, PPRV nucleic acid was detected in all 25 IcELISA-positive tested samples. 相似文献
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鲁桂侠 《中国动物传染病学报》2021,(2):88-92
为了掌握河北省羊肠道病毒(CEV)的流行现状,本研究采用RT-PCR方法对2017-2019年采集于河北省部分地区的266份羊粪便样品进行了CEV感染的病原学检测与分析。结果表明,266份粪便样品共检测出CEV阳性样品74份,CEV平均阳性感染率达27.82%,其中不同地区CEV阳性感染率为14.29%~37.14%,2017年、2018年、2019年CEV阳性感染率分别为21.62%、26.67%、33.33%,春季、夏季、秋季、冬季CEV阳性感染率分别为32.43%、14.29 %、34.09%、22.58 %,羔羊、育肥羊、种羊、后备种羊CEV阳性感染率分别为37.29%、14.26%、24.39%、15.79%,圈养、散养、半散养CEV阳性感染率分别为35.94%、15.38%、23.26%。调查与分析结果说明,河北省不同地区均存在不同程度的CEV感染,且阳性感染率存在逐年升高的趋势,秋季和春季相对于夏季和冬季羊群更容易发生CEV感染,羔羊相对于育肥羊和后备种羊更容易感染CEV,圈养方式相对于散养方式更容易发生CEV感染。 相似文献