绿汁发酵液和纤维素酶对香蕉茎+小麦麸混合青贮品质的影响

本试验旨在研究绿汁发酵液和纤维素酶对香蕉茎+小麦麸混合青贮发酵品质的影响。调制1个香蕉茎单贮(BSS)组和8个香蕉茎小麦麸混合青贮组,分别为1个对照(CON)组,添加2 mL/kg绿汁发酵液(FGJ)组,添加0.05、0.15和0.25 g/kg纤维素酶(CEL1、CEL2和CEL3)3个水平组,添加FGJ+CEL1(MIX1)、FGJ+CEL2(MIX2)和FGJ+CEL3(MIX3)3个混合组,每组均设3个重复。常温发酵60 d,测定青贮的发酵品质和化学成分。结果表明,与BSS组相比,CON组的气体损失率(GLR)和pH显著降低(P<0.05)。与CON组相比,各添加剂组的干物质(DM)、干物质回收率(DMR)和可溶性碳水化合物(WSC)含量有不同程度的增高,中性洗涤纤维(NDF)、酸性洗涤纤维(ADF)及氨态氮(AN)含量有不同程度的降低;与FGJ组相比,CEL和MIX组的DMR极显著升高(P<0.01)。在CEL组中,随CEL水平的增加,青贮效果愈好;与MIX1组相比,MIX3组的NDF含量显著减少(P<0.05),DMR显著升高(P<0.05);与CEL组相比,对应的MIX组有不同程度的改善。综上所述,在香蕉茎青贮中添加小麦麸有利于改善青贮品质,添加纤维素的效果优于添加绿汁发酵液的效果,二者混合效果更好,且混合后的青贮效果随着纤维素酶的增加而更好。     

绿汁发酵液对青贮玉米秸秆品质的影响

用收割后3、4、5、6d的玉米秸秆作原料调制了青贮。各种原料均设添加玉米面粉(CF)、绿汁发酵液(FGJ)、玉米面粉 绿汁发酵液(MIX)和对照(CON)4个处理。室温下贮存50d开封,测定青贮的化学成分和发酵品质。结果表明,3种添加剂对提高玉米秸秆青贮的品质均有效,特别是MIX的添加效果最佳;同时,也表明玉米秸秆收割后4d是最佳的青贮开始时间。     

绿汁发酵液对青贮玉米秸秆品质的影响

用收割后3、4、5、6d的玉米秸秆作原料调制了青贮。各种原料均设添加玉米面粉（CF）、绿汁发酵液（FGJ）、玉米面粉＋绿汁发酵液（MIX）和对照（CON）4个处理。室温下贮存50d开封,测定青贮的化学成分和发酵品质。结果表明,3种添加剂对提高玉米秸秆青贮的品质均有效,特别是MIX的添加效果最佳;同时,也表明玉米秸秆收割后4d是最佳的青贮开始时间。     

绿汁发酵液和纤维素酶对稻草青贮及稻草、甘蔗梢混合青贮品质的影响

本研究以稻草和甘蔗梢为青贮原料,调制稻草青贮及稻草与甘蔗梢混合青贮（混合比例为6：4（m/m））。在稻草青贮及稻草、甘蔗梢混合青贮中均设计添加2 mL/kg的绿汁发酵液组（FGJ）、3 mL/kg的纤维素酶组（CEL）、2 mL/kg绿汁发酵液+3 mL/kg纤维素酶的复合组（MIX）和对照组（CON）,每个处理重复3次,室温条件下贮存60 d后开封,测定青贮的发酵品质和化学成分。结果显示,青贮原料稻草干物质中的可溶性碳水化合物（WSC）和粗蛋白质（CP）含量均低于甘蔗梢,而中性洗涤纤维（NDF）、酸性洗涤纤维（ADF）含量均高于甘蔗梢。稻草单独青贮后,与CON组相比,FGJ组的NDF、ADF、pH和氨态氮均极显著降低（P<0.01）,WSC极显著增加（P<0.01）,干物质回收率（DMR）升高;MIX组的NDF、ADF、pH、气体损失率（GLR）和氨态氮均极显著降低（P<0.01）,乳酸、WSC及DMR升高;CEL组中除ADF显著降低外,其他指标也有不同程度的改善。与稻草青贮相比,稻草、甘蔗梢混合青贮的pH、氨态氮、NDF和半纤维素（HC）均降低,而DMR和WSC均明显升高。综上所述,绿汁发酵液及其与纤维素酶的复合添加剂对改善稻草青贮及稻草、甘蔗梢混合青贮品质的效果相近,且优于纤维素酶;稻草、甘蔗梢混合青贮的品质优于稻草青贮。     

绿汁发酵液对水稻秸青贮品质的影响

本试验利用绿汁发酵液作为青贮添加剂,研究其对水稻秸青贮品质的影响。结果表明,绿汁发酵液（PFJ）或其发酵液稀释再发酵后的绿汁发酵液（dPFJ）处理组青贮稻秸中的pH值和氨态氮水平明显低于其他处理组（P〈0.05）,乳酸含量也显著高于其他处理组。由此可见,PFJ和dPFJ能够明显地改善水稻秸青贮品质。     

添加绿汁发酵液对苜蓿青贮料发酵品质的影响

用未预干和预干的开花初期的苜蓿原料草，添加绿汁发酵液及将其稀释调制成的绿汁发酵液，探讨绿汁发酵液对青贮料发酵品质的影响，发见，除了未预干草中的dPFJ20绿汁发酵液的添加之外，其它的绿汁发酵液都明显改善了苜蓿青贮料的品质。     

绿汁发酵液对青贮发酵品质影响的研究进展

绿汁发酵液是近年来研制出的一种安全、有效、无污染、无公害、绿色的青贮添加剂。本文系统总结了在青贮发酵中添加绿汁发酵液的添加效果及其影响因素，提出了粉剂化的绿汁发酵液在生产实践中具有很大的推广应用前景。     

绿汁发酵液对柱花草青贮营养成分及品质的影响

试验旨在研究绿汁发酵液(previously fermented juice,PFJ)对柱花草青贮营养成分及品质的影响。以热研2号柱花草为原料进行青贮试验,设对照组(直接青贮)和试验Ⅰ、Ⅱ、Ⅲ、Ⅳ及Ⅴ组,各试验组PFJ添加比例为1%、2%、3%、4%和5%,30 d后测定柱花草青贮饲料主要营养成分含量及发酵品质。结果显示,与对照组相比,试验Ⅰ、Ⅱ、Ⅲ、Ⅳ组粗蛋白质(CP)含量显著提高(P < 0.05),Ⅱ、Ⅲ、Ⅳ和Ⅴ组中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)含量均有不同程度的降低,Ⅱ、Ⅲ、Ⅳ、Ⅴ组柱花草青贮的饲料相对值(RFV)显著提高(P < 0.05)。添加PFJ试验组与对照组相比显著降低了青贮饲料的pH (P < 0.05),乙酸和丙酸含量均有不同程度的降低,乳酸含量显著提高(P < 0.05)。柱花草直接青贮品质较差,添加PFJ提高了饲料青贮质量评分等级。综上所述,添加PFJ可提高营养价值,明显改善柱花草青贮品质,PFJ添加量为4%时青贮效果最好。     

Effects of Fermented Green Juice and Cellulase on the Fermentation Quality of Rice Straw Silage and Rice Straw with Sugarcane Tip Mixed Silage

Rice straw and sugarcane tip were used as the silage materials in this research,and there were two groups:Rice straw silage and mixed silage of rice straw and sugarcane tip with the mixed ratio 6:4(m/m).Four treatment groups were set up in each silage:Control group (CON) and three treatment groups which added 2 mL/kg fermented green juice (FGJ),3 mL/kg cellulase (CEL) and 2 mL/kg FGJ+3 mL/kg CEL (MIX),and treatment was repeated 3 times. The materials were ensiled at room temperature for 60 days,and the fermentation quality and chemical compositions were tested. The result showed that for the two kinds of raw silage materials,the contents of WSC and CP in rice straw were lower than those in sugarcane tip,while the contents of NDF and ADF in rice straw were higher than those of sugarcane tip.Compared with the CON group,the NDF,ADF,pH and NH₃-N in FGJ group were extremely significantly decreased when rice straw was silaged alone (P<0.01),while WSC was extremely significantly increased (P<0.01),and DMR was increased. The NDF,ADF,pH,GLR and NH₃-N in MIX group were extremely significantly decreased (P<0.01),while lactic acid,WSC and DMR were increased. In CEL group, the ADF was significantly decreased (P<0.05), and other indicators also had improved in different degrees. Compared with rice straw silage,the NDF,HC,pH and NH₃-N of mixed silage were lower,while DMR and WSC were obviously increased. In conclusion,the fermented green juice and it compound with cellulase had the similar effects on improving the fermentation quality,and the effects were both better than that of cellulase. The fermentation quality of rice straw with sugarcane tip mixed silage was better than rice straw silage.     

绿汁发酵液、纤维素酶和木聚糖酶对灵芝菌糟发酵饲料品质的影响

本试验旨在研究绿汁发酵液、纤维素酶和木聚糖酶对灵芝菌糟发酵饲料品质的影响,为利用菌糟生产优质发酵饲料提供理论依据.试验分为7个处理组,以添加蒸馏水为对照(CON组),灵芝菌糟中分别添加2 mL/kg绿汁发酵液(FGJ组)、2000 U/kg纤维素酶(CEL组)、5000 U/kg木聚糖酶(XYL组)、2 mL/kg绿汁发酵液+2000 U/kg纤维素酶(MIX1组)、2 mL/kg绿汁发酵液+5000 U/kg木聚糖酶(MIX2组)、2 mL/kg绿汁发酵液+2000 U/kg纤维素酶+5000 U/kg木聚糖酶(MIX3组),每个处理组3个重复,常温下发酵60 d开封,测定灵芝菌糟发酵饲料的化学成分和发酵品质.结果表明,与CON组相比,FGJ组、CEL组和XYL组可溶性碳水化合物(WSC)含量极显著升高(P<0.01),pH、气体损失率(GLR)及中性洗涤纤维(NDF)、半纤维素(HC)、氨态氮(AN)含量均极显著降低(P<0.01);与FGJ组相比,MIX1组和MIX2组乳酸(LA)、乙酸(AA)含量显著或极显著升高(P<0.05;P<0.01),WSC含量极显著升高(P<0.01),AN含量极显著降低(P<0.01).与CEL组相比,MIX1组的干物质回收率(DMR)及NDF含量极显著降低(P<0.01),WSC、AN含量极显著升高(P<0.01).与MIX1组和MIX2组相比,MIX3组pH、GLR极显著降低(P<0.01),酸性洗涤纤维(ADF)含量显著降低(P<0.05).综上所述,添加纤维素酶和木聚糖酶的效果均优于添加绿汁发酵液,但复合添加效果更佳,其中以MIX3组的添加效果最佳.     

添加绿汁发酵液和木聚糖酶对稻草青贮品质的影响

本试验旨在研究绿汁发酵液和木聚糖酶对稻草青贮品质的影响。在常规水分(MC1)稻草青贮和低水分(MC2)稻草青贮中均设对照(CON)组及添加2 mL/kg绿汁发酵液(FGJ)组、20 mg/kg木聚糖酶(XYL)组、2 mL/kg绿汁发酵液与20 mg/kg木聚糖酶的复合(MIX)组,每个处理组重复3次。常温下贮存60 d开封,测定青贮的发酵品质和化学成分。结果表明,MC1青贮中,与CON组相比,MIX组水分和酸性洗涤纤维(ADF)含量显著降低(P<0.05),FGJ、XYL、MIX组氨态氮(AN)含量极显著降低(P<0.01)、水溶性碳水化合物(WSC)含量显著或极显著增加(P<0.05;P<0.01),XYL、MIX组中性洗涤纤维(NDF)含量显著降低(P<0.05),FGJ组pH极显著降低(P<0.01);MC2青贮中,与CON组相比,FGJ组水分含量显著降低、水溶性碳水化合物含量及干物质回收率(DMR)显著增加(P<0.05),FGJ、MIX组氨态氮和中性洗涤纤维含量显著或极显著降低(P<0.05;P<0.01)、pH极显著降低(P<0.01),MIX组酸性洗涤纤维含量显著降低(P<0.05)。较MC1青贮,MC2青贮的pH和干物质回收率极显著增加(P<0.01),水溶性碳水化合物含量显著增加(P<0.05),氨态氮含量极显著降低(P<0.01)。综上所述,在常规水分和低水分稻草青贮中,3种添加剂均有显著的添加效果,以绿汁发酵液与木聚糖酶复合添加的效果最佳,且低水分稻草青贮的效果优于常规水分稻草青贮。     

Effects of Previously Fermented Juice on the Nutrient Composition and Quality of Stylo Silage

The experiment was carried out to investigate the effects of previously fermented juice (PFJ) on the nutrient composition and quality of stylo silage.The Stylosanthes guianensis Reyan No.2 were selected and there were six groups:Control group (to be silaged directly) and groups Ⅰ,Ⅱ,Ⅲ,Ⅳ and Ⅴ which were added with 1%,2%,3%,4% and 5% PFJ,respectively.All groups were stored for 30 days,and then the chemical composition and fermentation quality of the stylo silage were analyzed.The results showed that compared to control group,CP contents of groups Ⅰ,Ⅱ,Ⅲ and Ⅳ were significantly increased (P < 0.05) and NDF,ADF contents of groups Ⅱ,Ⅲ Ⅳ and Ⅴ were decreased in different degree.The RFV of groups Ⅱ,Ⅲ,Ⅳ and Ⅴ were significantly increased (P < 0.05).Compared with control group,the pH of the PFJ treatment groups were significantly decreased (P < 0.05),meanwhile acetic acid and propionic acid were decreased in different degree.Lactic acid contents were significantly increased (P < 0.05).The quality of stylo silage was poorer when it was silaged directly and the Flieg grades could be improved adding PFJ.In conclusion,adding PFJ could improve the fermentation quality and nutrient value of stylo silage and 4% PFJ was the proper application rate.     

纤维素酶对象草玉米秸秆混合青贮品质的影响

为探明纤维素酶（cellulase,CEL）对象草（Pennisetum purpureum Schum）和甜玉米（Zeamays L．var．rugosa Bona f．）秸秆混合青贮品质的影响。在象草：玉米秸秆为70：30（鲜样质量比）的混合原料中添加CEL进行青贮调制,添加水平分别为0％（对照）、0．001％（1U／g）、0．005％（5U／g）和0．025％（25U／g）。每个处理设3次重复。在常温条件下贮存60d,开封后测定青贮的pH、乳酸、乙酸、丙酸、丁酸,氨态氮等。结果表明,CEL能极显著减少青贮的中性洗涤纤维和半纤维素含量（P〈0．01）,极显著增加乳酸含量（P〈0．01）。在4个处理组中,0．005％CEL组干物质和干物质回收率最高,并且该处理组的pH、乙酸、氨态氮和酸性洗涤纤维极显著（P〈0．01）低于其他处理组,乳酸和可溶性碳水化合物极显著（P〈0．01）高于其他处理组。上述结果表明,添加0．005％CEL的处理效果最佳。     

布氏乳杆菌和纤维素酶对青贮玉米秸秆品质影响研究

为研究布氏乳杆菌和纤维素酶对青贮玉米秸秆粗饲料发酵品质和有氧稳定性的影响,分别设置CK组(空白对照组)、LB组(布氏乳杆菌组)、ENZ组(纤维素酶组)及LB+ENZ组(布氏乳杆菌和纤维素酶共同处理组)的玉米秸秆青贮发酵试验.发酵45 d后取样进行青贮发酵品质感官综合评价,测定pH值、有机酸含量、微生物活菌数、水溶性碳水...