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1.
    
Genetic parameters were estimated to investigate the relationships between meat quality traits and fatty acid composition from 11 855 Japanese Black cattle. The meat quality traits included beef marbling score (BMS), beef color score, firmness of beef (FIR), texture of beef (TEX) and beef fat color score (BFS). The data on fatty acid composition included oleic acid and monounsaturated fatty acids (MUFA) contents, the ratio of MUFA to saturated fatty acids (MUS) and the ratio of elongation. The heritability estimates for meat quality traits ranged from moderate (0.30) to high (0.72). The strong genetic correlations between them were useful for simultaneous genetic improvement. In addition, high heritability estimates of fatty acid composition, ranging from 0.60 to 0.63, indicated that they could also be improved genetically. The genetic correlations of fatty acid composition with BMS, FIR and TEX were weak and negative. In contrast, positive and stronger genetic correlations were found between BFS and fatty acid composition, in particular, related to the level of unsaturation (0.77 and 0.79 for MUFA and MUS, respectively). This implies that improving the level of unsaturation makes fat darker (more yellow) and thus requires balancing with BFS.  相似文献   

2.
    
Crossbreeding is an effective method of improving the efficiency and profit of production in commercial pig operations. To understand the effect of crossbreeding on meat and nutrient quality, a combination including three purebred (Duroc, D; Landrace, L; Yorkshire, Y) and two crossbred pig lines (Landrace × Yorkshire, LY; Duroc × (Landrace × Yorkshire), DLY) frequently used internationally were studied. The results showed that meat from the LY and DLY crosses had lower values for lightness , shear force and epinephrine and higher values for drip loss, C18:1, insulin, glucagon and monounsaturated fatty acids than D, L and Y pigs. Moreover, LY had higher values for post mortem pH and lower values for a* and b* than the purebreds. In contrast, DLY had lower values for pH and higher values for a* and b* than the purebreds. Meat quality‐related gene analysis showed that the CAST, IGF2 and MC4R gene expression levels in the LY and DLY pigs were significantly higher than those in the D, L and Y pigs. These results indicate that crossbreeding can alter the meat quality, nutritive value, energy metabolism and gene expression of pigs. Future research should focus on microRNA expression and DNA methylation that regulate gene expression and thus affect the meat quality.  相似文献   

3.
试验旨在分析研究乌珠穆沁草原饲养澳大利亚黑安格斯肉牛肌内脂肪酸含量和组成,为今后该品种的饲养改良、杂交繁育及肉品质的提高提供科学的基础数据.试验采集7头体重为(500±38)kg澳大利亚黑安格斯肉牛的里脊、臀肉、肩肉、眼肉、上脑、胸肉和米龙等不同部位肉样,采用简单高效脂肪酸提取方法,用气相色谱仪测定脂肪酸组成与含量,并与FAO/WHO推荐值比较,进行脂肪酸营养价值分析.结果表明黑安格斯肉牛不同部位牛肉中饱和脂肪酸(SFA)含量由多到少顺序为里脊、上脑、眼肉、肩肉、胸肉、臀肉及米龙,皆以棕榈酸和硬脂酸含量为主;单不饱和脂肪酸(MUFA)中油酸含量最高;检出的多不饱和脂肪酸(PUFA)多为亚油酸和亚麻酸;臀肉P/S和M/S最高,n-3/n-6 PUFAs比值达到0.85,接近FAO/WHO推荐值.以上结果表明乌珠穆沁草原饲养的黑安格斯肉牛的肉质风味良好,有较高的营养价值.  相似文献   

4.
Intramuscular fatty acids content and composition of Australian imported Black Angus beef raised in Ujinmqin grassland were analysised in this experiment to provide basic data for future breeding,crossbreeding,and meat quality evaluation.Target samples were taken of tenderloin,nates,shoulder clod,ribloin,highrib,brisket and topside from seven Black Angus beef which were Australian imported and raised in Ujinmqin grassland,with mean weights of (500±38)kg.The high efficient fatty acids extraction method and gas chromatograph (GC) were used to determine the content and composition of intramuscular fatty acids.Then the resuls were compared with FAO/WHO recommended values.The results showed that,the SFA content in target samples of Black Angus beef followed from high to low as:tenderloin,highrib,ribloin,shoulder clod,brisket,nates,topside.Moreover,majorities of intramuscular fatty acids composition were palmitic acid and stearic acid.The most highest content in MUFA was oleic acid;large amount of linoleic acid and linolenic acid were found in PUFA;The highest ratio of P/S and M/S were shown in nates,and the ratio of n-3/n-6 was 0.85.It was concluded that the meat of Australian imported Black Angus beef raised in Ujinmqin grassland had good flavor and high nutritive value.  相似文献   

5.
    
ABSTRACT

1. Fatty acid-binding proteins (FABP) are members of lipid-binding proteins, which participate in the metabolism and intracellular transportation of lipids. This study was designed to investigate the expression patterns, polymorphisms and associations with meat quality traits of the FABP1 gene in pigeons.

2. The temporal-spatial expression patterns showed FABP1 was widely expressed in all eleven tissues from 0–4 weeks of age, the expression level in the liver was the highest, followed by the small intestine and subcutaneous fat.

3. Five novel SNPs were found; all of them were synonymous and in Hardy-Weinberg equilibrium. Association analysis revealed that for the SNP of G161C, the AB and BB genotypes had higher (P ≤ 0.01) inosinic acid concentrations in breast muscle than the AA genotype. The BB genotype showed the highest (P < 0.01) intramuscular fat among the three genotypes, and significantly greater FABP1 mRNA levels were observed in the breast muscle of the BB genotype than in the AA and AB genotypes (P < 0.01). In the SNP C1376T, the AB and BB genotypes showed higher (P < 0.01) intramuscular fat than the AA genotype, and the relative mRNA expression level of the BB (P < 0.01) and AB (P < 0.05) genotypes was higher than that of the AA genotype in breast muscle. Correlation analysis implied that the FABP1 mRNA expression level was closely related to the inosinic acid (P < 0.05) and intramuscular fat content (P < 0.01). Oil red O staining of frozen sections of breast muscle on d 28 for SNPs G161C and C1376T also indicated that the BB genotype had the highest intramuscular fat content in both SNPs. In addition, correlation analysis implied the FABP1 mRNA expression level was closely related to inosinic acid (P < 0.05) and intramuscular fat content (P < 0.01).

4. The results suggested that FABP1 could be a potential candidate gene in marker-assisted selection for breeding pigeons with high-quality meat.  相似文献   

6.
    
The present study investigated the influence of a diet largely comprising rice whole‐crop silage (rWCS) on growth performance, carcass and meat characteristics, and expression of genes involved in muscle growth of Japanese Black steers. Steers were randomly separated into rWCS‐fed (rWCS ad libitum and restricted feeding of concentrate) and concentrate‐fed groups. Total digestible nutrient intake and daily gain (DG) decreased in rWCS‐fed steers in comparison with concentrate‐fed steers, whereas dressed carcass weight and final body weight did not significantly differ between the groups. Decreases in drip loss in the muscle of rWCS‐fed steers may be caused by α‐tocopherol and β‐carotene in muscle. Feeding large amounts of rWCS to steers may maintain quantitative productivity of beef steers equally to a concentrate‐based diet, and improve the qualitative productivity. Results of gene expression suggest that activation of skeletal muscle growth in rWCS‐fed steers may occur at the late fattening period owing to a decrease in myostatin and increase in myosin heavy chain gene expression. Preadipocyte factor‐1 and myostatin genes may be strongly involved in the control of lipid accumulation. This rearing system would allow beef production to switch to rWCS‐based diets from concentrate‐based diets.  相似文献   

7.
    
The present review has been focused largely on the sex type differences in beef quality among heifers, cows, steers and bulls in various feeding environments. Genetic groups, feeding systems and gender are the major factors that change carcass characteristics and fatty acid profiles of cattle. Studies identified that heifer beef has super characteristics in eating quality and a better healthy composition in fatty acids than steer, cow and bull. Diet influences the variation of fatty acid profile; particularly the level of polyunsaturated fatty acids (PUFA) interacts with breed and sex. Animals finished in pasture systems were reported to show better ratios of PUFA/ saturated fatty acids and n‐6/n‐3. Carcasses of roughage‐fed beef are lighter and have less marbling and lower quality grades but have higher cutability than carcasses of grain‐fed bulls. Heifers and cows are reported to deposit more fat than steers and bulls. Among males, lower production of testosterone by steers favors more fat thickness compared with bulls. Marbling greatly varies among cattle belonging to different sexes, and particularly, females have genetic makeup that efficiently controls deposition. The current review identified that heifers can be a premium beef brand, while steer beef currently take a large part of market share across the world.  相似文献   

8.
    
The current study examines the effect of different finishing diets (hay‐ vs. maize‐silage on meal ration) on carcass quality, physical, chemical and sensory properties, and fatty acid profiles of buffalo meat. Twenty male Italian Mediterranean buffaloes (246 ± 9.00 kg live weight) were distributed at random into two groups at the beginning of the finishing period (368 ± 20 days). The buffaloes were offered two finishing diets: a maize silage (MS) or an alfalfa hay (AH) diet. No significant differences were found between dietary treatments for live and carcass weight. Meat chemical composition was influenced by dietary treatment. A higher fat content was detected in meat from animals finished with MS than AH (P < 0.05). Overall, the data indicated differences between the fatty acid profiles of meat as a consequence of different feeding systems. The higher fat deposition in the MS group resulted in meat with a less favorable fatty acid profile (i.e. a lower polyunsaturated/saturated fatty acid ratio and α‐linolenic fatty acid content) in relation to human health compared with meat from animals fed the AH diet.  相似文献   

9.
本试验旨在研究鸭油对生长肥育猪生长性能、肉品质及肌肉脂肪酸沉积的影响。选用初始平均体重为(31.62±2.31)kg的“杜长大”去势公猪72头,按体重相近原则随机分为2组,每组设6个重复,每个重复6头猪,2组饲粮均为玉米-豆粕型,在生长期饲粮中分别添加2%的豆油和鸭油,而在肥育期饲粮中分别添加1%的豆油和鸭油。试验期为82 d。结果表明:与豆油组相比,1)饲粮中添加鸭油显著提高了肥育期总能和干物质的表观消化率(P<0.05)。2)饲粮中添加鸭油显著提高了背最长肌肌内脂肪(IMF)含量(P<0.05),显著降低剪切力(P<0.05),并显著降低了肥育期血清中总胆固醇、甘油三酯以及低密度脂蛋白胆固醇的含量(P<0.05)。3)对背最长肌脂肪酸组成分析发现,饲粮中添加鸭油显著增加了C18∶0含量(P<0.05),但同时显著降低了C18∶2和总多不饱和脂肪酸的含量(P<0.05),使背最长肌中多不饱和脂肪酸/饱和脂肪酸显著降低(P<0.05)。由此可见,本试验中鸭油添加量不会对生长肥育猪的生长性能产生负面影响,且可改善瘦肉型猪的IMF含量及嫩度,对提升肉品风味有积极作用。  相似文献   

10.
    
The objective of this work was to analyse the porcine Fatty acid binding protein 2, intestinal ( FABP2 ) gene as a candidate gene for a fatty acid composition quantitative trait loci (QTL) previously described on porcine chromosome 8 in an Iberian by Landrace F2 cross (IBMAP). Re-sequencing of the porcine FABP2 gene in three Iberian and eight Landrace parental animals resulted in the identification of three single-nucleotide polymorphisms, all of them localized in intron 1. The polymorphism FABP2 :g.412T>C, localized in intron 1, and two additional microsatellites were genotyped in the IBMAP population in order to perform an association test of the FABP2 gene and to better define the QTL position previously described. Association analyses of the FABP2 :g.412T>C with the fatty acid composition traits were not significant in simple association and marker-assisted association tests, suggesting that the FABP2 region sequenced is not responsible for the QTL. However, the addition of three new markers to the pedigree allowed us to define the S0144–SW61 marker interval as the most likely QTL position, facilitating the future study of other candidate genes for this QTL.  相似文献   

11.
以40￣90kg6个体重阶段莱芜猪和新莱芜猪为试验对象(n=6),研究肌肉脂肪酸组成的发育性变化及其与肉质特性的关系。结果表明:①随着体重的增大,莱芜猪肌肉总脂含量显著(P<0.01)增加;饱和脂肪酸(SFA)总量的比例有下降的趋势,不饱和脂肪酸(UFA)总量有增加的趋势,但差异不显著;在UFA中,多不饱和脂肪酸(PUFA)总量及亚油酸的比例显著(P<0.01)下降,油酸比例显著(P<0.01)增加;②总体上,莱芜猪肌肉中的SFA+单不饱和脂肪酸总量、总脂、棕榈酸、棕榈烯酸的比例显著高于新莱芜猪,而PUFA总量、硬脂酸、亚油酸、花生烯酸的比例显著(P<0.01)低于新莱芜猪。各体重阶段2个品种间的脂肪酸组成也存在着较大的差异;③莱芜猪肌肉中的PUFA总量与总脂、水分、失水率、大理石纹评分相关显著;总脂与水分、大理石纹相关极显著(P<0.01);SFA、UFA总量与大理石纹呈显著(P<0.05)相关。  相似文献   

12.
    
In this study, meat quality traits were compared between squabs from two pigeon breeds: one Chinese indigenous breed, the Shiqi (SQ) meat‐type pigeon, and an imported breed, the white king (WK) meat‐type pigeon. Breed differences were detected in the content of intramuscular fat (IMF) in the breast muscle. SQ squabs had significantly higher IMF content than the WK birds. The shear force value (an objective measure of meat tenderness) of SQ birds was also relatively lower than that of the WK squabs. Further analysis of fatty acids profile revealed that SQ squabs exhibited significant advantage in the synthesis of polyunsaturated fatty acids, while WK squabs were significantly higher in the sum of monounsaturated fatty acids. Breast muscle in the SQ squabs was also significantly higher in the ratio of polyunsaturated fatty acids to saturated fatty acids, as well as the sum of omega 6 fatty acids. Variability of expression levels of functional genes in relation to fat accumulation and meat tenderness was analysed by qRT‐PCR. Gene expression analyses showed that the hepatic expression of LPL (lipoprotein lipase), FABP4 (fatty acid‐binding protein 4), and CAPN2 (calpain‐2) were significantly higher in the SQ squabs. In the breast muscle tissue, the FABP3 (fatty acid‐binding protein 3) and CAPN2mRNA abundance was significantly higher in SQ squabs. Our results suggested that these differentially expressed genes might be candidate genes used in the programmes of targeted selection for squabs with higher IMF content, tender meat, and more favourable fatty acids composition.  相似文献   

13.
本试验旨在研究补饲精料对西藏彭波半细毛羊羊肉品质的影响。选择30只成年彭波半细毛羊母羊,按照同质原则随机分为3组:放牧不补饲(对照,G1)组、补饲精料200 g/d(G2)组、补饲精料400 g/d(G3)组,每组10只。每天19:30补饲,正试期75 d。正试期结束屠宰,采集背最长肌测定营养物质含量和组织结构特征。结果表明:高水平补饲精料提高了母羊总干物质采食量,G3组平均日增重显著高于G1、G2组(P0.05);补饲精料对羊肉p H1 h、p H24 h、滴水损失及水分和粗灰分含量无显著影响(P0.05),但G3组的熟肉率,粗蛋白质、肌内脂肪含量,肌纤维密度显著高于G1组(P0.05);G2、G3组羊肉总氨基酸、必需氨基酸和天冬氨酸含量显著高于G1组(P0.05),G3组苯丙氨酸、赖氨酸含量显著高于G1组(P0.05),G3组苏氨酸、谷氨酸含量有高于G1组的趋势(0.05≤P0.10);补饲精料对羊肉饱和脂肪酸、单不饱和脂肪酸和多不饱和脂肪酸含量没有显著影响(P0.05),G3组C18∶1 trans-9含量有低于G1组的趋势(0.05≤P0.10)。上述结果表明,补饲精料能显著提高彭波半细毛羊羊肉营养价值,改善肉质嫩度,每只补饲400 g/d优于200 g/d。  相似文献   

14.
    
The aim of this study was to compare the meat quality and evaluate the chemical composition of Chinese Ningdu yellow chicken of different weights once they have reached market age. Thirty hens at the day of age 118 were selected and divided into three groups according to their weight: light weight (1288.00 ± 69.78 g, n = 10), medium weight (1407.17 ± 39.40 g, n = 10), heavy weight (1581.6 ± 46.59 g, n = 10), and the differences in weight among these three groups are significant. Biochemical, histological, and metabonomic approaches were used to obtain index values of meat quality and chemical composition. Compared with meat from lighter chickens, muscle fiber density was significantly lower in heavier chickens, and meat pH was positively correlated with chicken weight. Though the amount of all measured amino acids were not different among three weight groups of chicken, the levels of several kinds of fatty acids exhibited significant differences or correlations, including linolenic acid, arachidonic acid, myristic acid, oleic acid, and docosahexaenoic acid (DHA). These results contribute to help customers choose the optimal chicken weight depending upon the food to be cooked.  相似文献   

15.
    
NREP (neuronal regeneration related protein homolog) plays a role in the transformation of neural, muscle, and fibroblast cells and in smooth muscle myogenesis. The NREP gene was selected for detailed study as an expressional and functional candidate gene on the basis of data from the expression microarray, which detected the differences in gene expression between Czech Large White pigs and wild boars in the longissimus lumborum et thoracis and biceps femoris muscle tissues. Quantitative real-time PCR results confirmed that porcine NREP was expressed in both skeletal muscles and significantly overexpressed in Czech Large White pigs compared with wild boars (14.5- and 11.6-fold; p < .05). We identified 9 polymorphic sites in the genomic DNA of NREP. Six of these polymorphisms were in complete linkage disequilibrium, and therefore, only 4 loci were informative. The associations of the HF571253:g.103G>A, HF571253:g.134G>A, HF571253:g.179T>C and HF571253:g.402_409delT polymorphisms with backfat thickness, lean meat content and average daily gain were assessed in Czech Large White pigs. The GG genotypes HF571253:g.103G>A and HF571253:g.134G>A, the TT genotypes HF571253:g.179T>C and 67 HF571253:g.402_409delT genotypes had favourable effects on the studied traits. Our results indicate the possibility of utilizing the variability of the NREP gene in marker-assisted selection in order to improve meat production in pigs.  相似文献   

16.
本试验旨在研究饲粮精氨酸(Arg)水平对肉鸡肉品质的影响。试验选用1日龄罗斯308肉鸡192只,公母各占1/2,按体重相近原则分成4个处理,每个处理8个重复,每个重复6只鸡。4个处理的饲粮在玉米-豆粕型基础饲粮中分别添加0(对照)、0.3%、0.6%、0.9%的L-Arg。试验期42 d。结果表明:饲粮Arg水平可线性提高肉鸡腿肌率(P0.50),饲粮中添加0.6%Arg的肉鸡的腿肌率最高,可较对照处理提高12.08%。饲粮Arg水平有线性降低肉鸡腹脂率的趋势(P0.10),饲粮中添加0.9%Arg的肉鸡的腹脂率最低,可较对照处理降低10.20%。饲粮Arg水平可线性降低腿肌中粗脂肪含量(P0.01),其中饲粮中添加0.6%Arg的肉鸡腿肌中粗脂肪含量最低,可较对照处理降低21.64%。饲粮Arg水平对腿肌的硬度(P0.01)、弹性(P0.01)和胸肌的硬度(P0.10)、弹性(P0.05)呈先降低后升高的二次曲线变化,饲粮添加0.3%Arg时,肉鸡腿肌的弹性和胸肌的硬度、弹性可降低至最低,分别较对照处理降低了46.34%、10.24%、41.79%。饲粮Arg水平对肉鸡腿肌中C16∶0、C16∶1、C18∶1、C18∶0含量(P0.05)和C20∶0、C20∶3含量(P0.10)呈先降低后升高的二次曲线变化。饲粮添加0.6%Arg时,肉鸡腿肌中C16∶0、C16∶1、C18∶1、C18∶0、C20∶0、C20∶3含量最低,分别较对照处理降低了48.58%、52.67%、48.40%、46.72%、54.08%、34.29%。饲粮中适当添加Arg可提高肉鸡腿肌率,有降低肉鸡腹脂率的趋势,可降低肉鸡腿肌中粗脂肪和部分脂肪酸含量,降低腿肌、胸肌的硬度和弹性。  相似文献   

17.
脂肪酸结合蛋白（FABPs）基因多态性对畜产品品质的影响   总被引：2，自引：0，他引：2  
脂肪酸结合蛋白（FABPs）属于脂结合蛋白超家族成员,广泛存在于动物细胞质中,承担着细胞内脂肪酸的运输任务。研究发现,脂肪酸结合蛋白（FABPs）基因多态性可以通过调控脂肪代谢来改善畜产品品质,提高饲料利用率,降低环境污染。就FABPs的结构和类型,FABPs对脂肪代谢的影响,FABPs基因多态性及其应用后在改善畜产品品质中发挥的作用、功效和应用特点进行了阐述,以期为改进畜禽养殖技术提供参考。  相似文献   

18.
以柠檬酸合成酶基因（citrate synthetase,CS）为候选基因,分析了该基因外显子3的遗传多态性与西门塔尔牛肉质性状的相关关系。选用95头西门塔尔牛为试验材料,采用PCR-RFLP的方法对该基因的外显子多态性进行检测,并利用SPSS软件对该外显子多态性与肉质性状的相关性进行分析。结果表明：CS基因第三外显子第206bp处存在G/A突变,该基因多态性与脂肪颜色呈显著相关,AA型显著高于BB型。对其他性状的影响差异不显著。通过研究CS基因外显子的多态性,为西门塔尔牛利用该基因的多态性进行分子育种提供了试验依据。  相似文献   

19.
    
Focus of this study was to evaluate the most suitable breed/crossbred and the appropriate nutritional strategies to increase marbling in beef muscle and to improve its healthy properties, in particular the n‐3 fatty acids content. One hundred and seventy‐six heifers of three crossbreed commonly reared by Emilia‐Romagna farmers: 48 Bleu Belge × Freisian (ITA), 48 Charolais × Aubrac (FRA), and 80 Angus (ANG) were used. Animals of each breed were randomly subdivided in two experimental groups that received two diets, differing for the dietary lipid source. Control group (C) received a basal diet containing protected vegetable fats, whereas treated one (T) received the same basal diet supplemented with 0.9 kg/head/day of extruded flaxseed. After slaughtering, a sample of Longissimus thoracis was collected from each animal for meat quality analysis. Our results demonstrated that, in a shorter fattening period, ANG animals obtained the best dry matter intake, average daily gain and the best fattening scores. ITA and ANG meat presented the highest marbling scores. ANG breed had the highest amount of C18:1, the highest unsaturated/saturated fatty acids ratio and the lowest n‐6/n‐3 ratio. The T animals, independently from breed, showed the highest amount of α‐linolenic acid (ALA). In addition, ANG‐T meat presented the highest ALA content.  相似文献   

20.
    
We identified a potential molecular marker associated with meat quality traits in the myosin heavy chain 4, MYH4 gene of Landrace pigs. Sequencing revealed a single nucleotide polymorphism (SNP; g.‐1398G>T) in the 5' upstream region of MYH4. It was significantly associated with the number of type IIa muscle fibers and water‐holding capacity based on filter‐paper fluid uptake. The GG genotype groups had a greater number of type IIa fibers and a larger area composed of type IIa fibers than the other genotype group (P = 0.004 and P = 0.061, respectively). Expression level of MYH4 gene in the genotype TT or GT was higher than in genotype of GG (P < 0.0001). The T allele may enhance expression level of MYH4 gene and then the portion of IIb type fiber in the muscle be increased by the T allelle. Therefore, we suggest that the g.‐1398G>T in the 5' upstream region of the porcine MYH4 may be used as a molecular marker for meat quality traits, although its functional effect is not defined yet.  相似文献   

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