蒙古绵羊妊娠后期营养限饲对胎儿肝脏急性期蛋白合成的影响

旨在研究妊娠后期营养限饲蒙古绵羊对其胎儿肝脏急性期蛋白合成的影响。选择健康的蒙古绵羊(经同期发情受孕)24只,在妊娠90d时选择6只母羊进行屠宰,其余18只随机均分为3个营养水平组:(0.175 MJ/kg BW~(0.75)·d,RG1组)、(0.33 MJ/kg BW~(0.75)·d,RG2组)和自由采食组(0.67 MJ/kg BW~(0.75)·d,CG组),妊娠140d时,对每组6只母羊进行屠宰。结果:RG1组胎儿肝脏重(P0.01)、肝脏中IL-1β、IL-2、INF-γ干扰素、结合珠蛋白和铜蓝蛋白显著低于CG组(P0.05),C反应蛋白显著高于CG组(P0.05),IL-4、肿瘤坏死因子(TNF)和淀粉样蛋白A差异不显著;RG2组胎儿肝脏重、肝脏中IL-1β、IL-2和TNF显著低于CG组(P0.05),而C反应蛋白极显著高于CG组(P0.01)。结果提示,妊娠后期营养限饲蒙古绵羊严重限制了其胎儿肝脏生长发育,且结构和功能也不同程度受到影响,RG1组严重影响了胎儿肝脏急性期蛋白合成,且机体适应性调控系统发生紊乱。     

Dietary N-carbamylglutamate or L-arginine improves fetal intestinal amino acid profiles during intrauterine growth restriction in undernourished ewes

Our previous studies demonstrated that prenatal in utero growth restriction impairs postnatal intestinal function.Thus,improving postpartal intestinal absorption capacity and growth by manipulating the maternal diet prepartum is of importance.This work was conducted to determine whether supplementation of N-carbamylglutamate(NCG)or rumen-protected L-arginine(RP-Arg)increased fetal intestinal amino acid(AA)profiles in intrauterine growth retardation(IUGR)fetuses.On d 35 of gestation,Hu ewes(n=32)carrying twin fetuses were randomized into 4 groups(8 ewes and 16 fetuses in each group),where diets were as follows:100%of nutrient requirements recommended by National Research Council(NRC,2007)(CON);50%of nutrient requirements recommended by NRC(2007)(RES);RES+RPArg(20 g/d),(RES+ARG);and RES+NCG(5 g/d),(RES+NCG).On d 110 of gestation,both fetal and maternal tissues were collected and weighed.Compared with RES,solute carrier family 1,member 5(SLC1A5)was upregulated(P<0.05)within fetal jejunum,duodenum and ileum when supplementing NCG and RP-Arg.Relative to RES,RP-Arg or NCG supplementation to RES resulted in upregulation(P<0.05)of peptide transporter 1 protein abundance within the fetal ileum.NCG or RP-Arg supplementation to RES also upregulated phosphorylated mechanistic target of rapamycin(pmTOR)-to-mTOR ratio in the fetal ileum induced by IUGR(P<0.05).As a result,during IUGR,supplementation of Arg or NCG affected intestinal AA profiles in the fetus in part through controlling mTOR signal transduction as well as AA and peptide transport.Future studies should be conducted to understand the role(if any)of the placenta on the improvement of growth and AA profiles independent of the fetal intestine.This would help demonstrate the relative contribution of intestinal uptake in fetal life.     

妊娠后期限饲对母羊肝脏功能及抗氧化的影响

本研究旨在评估母羊妊娠后期限饲对其肝脏功能及抗氧化性能的影响。试验选择平均体重为(52.82±2.72)kg的妊娠后期母羊(2~3胎次)36只,随机分为3组,每组6个重复,每个重复2头羊。各组分别饲喂对照组日粮(代谢能:0.68 MJ/kg代谢体重/d)、处理1组日粮(代谢能:0.17 MJ/kg代谢体重/d)、处理2组日粮(代谢能:0.34 MJ/kg代谢体重/d),试验从妊娠90 d持续至140 d。结果:处理1组母羊肝脏脂肪含量较对照组和处理2组显著提高了288.03%和282.48%(P<0.05)。与对照组相比,处理1组肝脏水分、灰分和蛋白质占比显著降低19.28%、44.32%和45.83%(P<0.05),但处理1组肝脏脂肪占比较对照组显著提高210.19%(P<0.05)。处理1组和处理2组血清非酯化脂肪酸含量较对照组分别提高199.94%和185.51%(P<0.05)。与对照组相比,处理1组血清β-羟丁酸和肝脏甘油三酯含量显著提高392.45%和329.55%(P<0.05)。处理1组肝脏超氧化物歧化酶、过氧化氢酶和总抗氧化力活性较对照组显著提高103.32%、83.36%和75.85%(P<0.05),同时处理1组丙二醛含量较对照组和处理2组显著提高54.52%和67.31%(P<0.05)。结论:妊娠后期限制母羊采食量会导致肝脏重量和蛋白质含量下降,同时引起肝脏脂肪的积累,肝脏抗氧化功能失衡。     

酵母培养物对断奶仔猪生长性能、机体抗氧化能力和免疫功能的影响

文章旨在研究酵母培养物在动物生产上的实际使用效果,试验选取断奶仔猪300头(21日龄断奶),随机分成3组,每组5个重复,每个重复20头仔猪,单栏饲养.分别饲喂基础日粮、基础日粮+1％的酵母培养物、基础日粮+100 mg/kg的金霉素,试验期为42 d.结果表明,在饲料中调价酵母培养物断奶仔猪的末重、平均日增重得到显著提...     

改性蒙脱石对肉鸭生长性能、免疫功能和抗氧化能力的影响

试验旨在研究改性蒙脱石(MMT)对肉鸭生长性能、免疫功能和抗氧化能力的影响。选取1日龄樱桃谷肉鸭320只,采用单因素随机区组试验设计,随机分为4组,每组8个重复,每个重复10只肉鸭。对照组肉鸭饲喂基础日粮,MMT低量组、MMT中量组和MMT高量组分别在基础日粮中添加600、1 200、2 400 mg/kg MMT。试验期42 d。结果显示,与对照组相比,MMT中量组和MMT高量组肉鸭平均日增重显著提高(P<0.05),料重比显著降低(P<0.05),血清白细胞介素-2 (IL-2)含量和总超氧化物歧化酶(T-SOD)活性显著提高(P<0.05),血清丙二醛(MDA)含量显著降低(P<0.05)。MMT低量组、MMT中量组和MMT高量组血清谷胱甘肽过氧化物酶(GSH-Px)活性和干扰素-γ(IFN-γ)含量显著高于对照组(P<0.05)。研究表明,添加MMT可以提高肉鸭的生长性能、免疫功能和抗氧化能力;1 200 mg/kg MMT饲喂肉鸭的效果较理想。     

琼胶多糖对异育银鲫生长、体组成和抗氧化能力的影响

为评估琼胶粉替代异育银鲫饲料中淀粉对异育银鲫生长和抗氧化性能的影响,试验以不含琼胶粉的基础饲料为对照组饲料,以1％(D1组)、3％(D2组)、5％(D3组)琼胶粉替代淀粉配制成的饲料为试验组饲料(所有饲料均等氮等脂),饲喂规格一致的异育银鲫幼鱼[初始重(10.32±0.16)g],每组3个重复,每个重复25尾试验鱼,饲...     

Effects of Bacillus subtilis on jejunal integrity,redox status,and microbial composition of intrauterine growth restriction suckling piglets

The present study used intrauterine growth restriction (IUGR) piglets as an animal model to determine the effect of Bacillus subtilis on intestinal integrity, antioxidant capacity, and microbiota in the jejunum of suckling piglets. In total, 8 normal birth weight (NBW) newborn piglets (1.62 ± 0.10 kg) and 16 newborn IUGR piglets (0.90 ± 0.08 kg) were selected and assigned to three groups. Piglets were orally gavaged with 10-mL sterile saline (NBW and IUGR groups), and IUGR piglets were orally gavaged with 10-mL/d bacterial fluid (B. subtilis diluted in sterile saline, gavage in the dose of 2 × 10⁹ colony-forming units per kg of body weight; IBS group; n = 8). IUGR induced jejunal barrier dysfunction and redox status imbalance of piglets, and changed the abundances of bacteria in the jejunum. Treatment with B. subtilis increased (P < 0.05) the ratio of villus height to crypt depth (VH/CD) in the jejunum, decreased (P < 0.05) the plasma diamine oxidase (DAO) activity, and enhanced (P < 0.05) the gene expressions of zonula occludens-1 (ZO-1), occludin, and claudin-1 in the jejunum of IUGR piglets. Treatment with B. subtilis decreased (P < 0.05) the concentration of protein carbonyl (PC) and increased (P < 0.05) the activities of catalase (CAT) and total superoxide dismutase (T-SOD) in the jejunum of IUGR piglets. Treatment with B. subtilis also increased (P < 0.05) gene expressions of superoxide dismutase 1 (SOD1), CAT, and nuclear factor erythroid 2-related factor (Nrf2), as well as the protein expressions of heme oxygenase-1 (HO-1), SOD1, and Nrf2 in the jejunum of IUGR piglets. Treatment with B. subtilis also improved the abundances and the community structure of bacteria in the jejunum of IUGR piglets. These results suggested that IUGR damaged the jejunal barrier function and antioxidant capacity of suckling piglets, and altered the abundances of bacteria in the jejunum. Treatment with B. subtilis improved the intestinal integrity and antioxidant capacity while also improved the abundances and structure of bacteria in the jejunum of suckling piglets.     

精氨酸对笼养育雏期蛋鸭生产性能及抗氧化功能的影响

试验旨在研究饲粮中添加不同水平精氨酸对0~4周龄笼养蛋雏鸭生长性能及抗氧化功能的影响,探讨笼养育雏期蛋鸭适宜的精氨酸添加水平。试验采用单因素完全随机分组设计,选择健康1日龄金定蛋雏鸭180只,随机分为5组,每组6个重复,每个重复6只鸭,对照组饲喂基础日粮,试验组饲喂在玉米-豆粕型基础饲粮基础上分别添加0.10%、0.20%、0.30%、0.40%精氨酸的试验饲粮,试验期4周。结果表明：①饲粮中添加精氨酸平均日增重Ⅲ组显著高于其他各组(P<0.05),Ⅱ、Ⅳ、Ⅴ组间差异均不显著(P>0.05),Ⅱ、Ⅳ组显著高于Ⅰ组(P<0.05);平均日采食量以Ⅰ组最高,但各组间差异均不显著(P>0.05);料重比以Ⅲ组最低,显著低于其他各组(P<0.05),Ⅱ、Ⅳ、Ⅴ组间差异不显著(P>0.05),同时,Ⅱ、Ⅳ、Ⅴ组显著低于对照组(P<0.05)。②Ⅲ组血清和肝脏总抗氧化能力(T-AOC)与对照组相比差异显著（P<0.05),Ⅱ组血清谷胱甘肽过氧化物酶(GSH-Px)活性与对照组相比差异显著(P<0.05);Ⅳ组肝脏超氧化物歧化酶(SOD)活性与对照组相比差异显著(P<0.05);Ⅱ、Ⅲ、Ⅳ组肝脏丙二醛(MDA)含量与对照组相比差异显著(P<0.05),根据试验中有效评价精氨酸营养需要量的抗氧化指标,估测精氨酸添加水平分别为0.183%、0.198%、0.093%、0.208%。由此可见,蛋雏鸭采食玉米-豆粕型基础饲粮,精氨酸的适宜添加水平为0.093%~0.208%。     

盐胁迫对高羊茅生长及抗氧化系统的影响

研究了不同浓度NaCl(0,50,100,200,300 mmol/L)胁迫对高羊茅生长及抗氧化系统的影响。结果表明,中、低浓度(50,100 mmol/L)盐胁迫下,高羊茅生物量和MDA含量与对照组相比没有显著变化;高浓度(200,300 mmol/L)盐胁迫后,生物量明显降低,MDA含量显著升高。抗氧化酶(SOD、POD、CAT)活性随着盐胁迫时间的延长先上升后下降,盐浓度越高3种酶活性下降幅度越大。盐胁迫初期,除类胡萝卜素含量略有上升外,GSH和还原型AsA均有不同程度的下降,随着胁迫时间的延长,3种抗氧化物质含量逐渐降低,200,300 mmol/L盐处理下降明显。     

模拟酸雨对吊竹梅生长及抗氧化能力的影响

采用盆栽方法,对吊竹梅用pH值为6.7(对照)、5.6、4.5、3.5、2.5、1.5的模拟酸雨进行喷雾处理,测定其生物量、花青素相对浓度、叶绿素含量、硝酸还原酶活性(nitrate reductase, NR)、脯氨酸含量、超氧化物歧化酶(superoxide dismutase, SOD) 活性、过氧化物酶 (peroxidase, POD)活性、过氧化氢酶(catalase, CAT)活性、丙二醛(malondialdehyde, MDA)含量和质膜透性等指标。结果显示,吊竹梅单株干质量、叶绿素含量、NR活性、花青素相对浓度、CAT活性均呈下降趋势;SOD活性呈先上升后下降趋势;脯氨酸含量、POD活性、MDA含量和质膜透性均呈上升趋势。综合分析表明,模拟酸雨pH值为5.6~3.5处理,吊竹梅生长和抗氧化能力受影响较小,而观赏性不受影响;模拟酸雨pH值为2.5处理,其生长受抑制程度和抗氧化能力下降幅度增大,对其观赏性几乎无影响;模拟酸雨pH值为1.5处理,其生长和抗氧化能力严重受抑制,有明显伤害症状,观赏性急剧下降。     

山楂提取物对断奶仔猪生长性能、免疫功能、抗氧化能力及肠道微生物的影响研究

为探讨不同添加量山楂提取物(CF)对断奶仔猪生长性能、免疫功能、抗氧化能力和肠道微生物的影响,试验选择体重为(8.85±0.73)kg左右、健康状况良好的28日龄"杜×长×大"断奶仔猪30头,按体重相近的原则随机分为5组,每组6个重复,每个重复1头仔猪.对照组(CON组)饲喂基础日粮,抗生素组(CTC组)是在基础日粮中...     

黄曲霉毒素对猪生长性能及免疫和抗氧化指标的影响

选用60头体质量28 kg左右的“杜长大”三元杂交猪,随机分为2组,每组3个重复,每个重复10头猪（公母各半）,分别饲喂基础日粮（C组）、基础日粮添加0.1 mg/kg黄曲霉毒素（AF组）,试验期90 d。饲养试验结束时,对猪的生长性能、免疫指标和抗氧化指标进行分析。结果表明：黄曲霉素显著影响试验猪的生长性能,日增重降低12.90%（P〈0.01）,料重比提高7.54%（P〈0.01）,同时显著降低试验猪的免疫功能和抗氧化能力。提示：长期饲喂0.1 mg/kg黄曲霉素可诱发猪黄曲霉毒素慢性中毒。     

马齿苋粉对肉鸡生长性能和抗氧化能力的影响

文章旨在研究饲粮中添加马齿苋粉对肉鸡生长性能和抗氧化能力的影响。试验选取平均体重接近的1日龄AA肉鸡160只,称重后随机分为4组,试验1、2、3组在对照组饲粮的基础上分别添加0.30%、0.60%、0.90%的马齿苋粉,共处理42 d。结果发现,与对照组相比,肉鸡21日龄时,试验1、2、3组ADG分别增加5.5%、10.0%、6.8%(P <0.05),试验1、2组F/C分别降低8.2%、6.4%(P <0.05);42日龄时,试验1、2、3组ADG分别增加8.9%、9.6%、8.7%(P <0.05),F/C分别降低8.6%、9.6%、10.1%(P <0.05)。此外,21日龄时,试验1、2、3组肉鸡血清中T-AOC比对照组分别增加12.0%、14.4%、10.1%(P <0.05),T-SOD活性分别增加6.6%、10.5%、9.1%(P <0.05),MDA浓度分别降低6.3%、8.0%、5.6%(P <0.05);42日龄时,试验1、2、3组肉鸡血清中T-AOC比对照组分别提高13.9%、20.3%、13.6%(P <0.05),T-SOD活性分别提高10.5%、14.8%、9.5%(P <0.05),MDA浓度分别降低11.1%、14.9%、9.7%(P <0.05),试验1、2组血清中GSH-Px活性较对照组分别提高10.2%、11.8%(P <0.05)。结果表明,饲粮中添加马齿苋粉显著促进肉鸡生长及增强抗氧化能力。     

Effects of dietary methionine supplementation on growth performance,intestinal morphology,antioxidant capacity and immune function in intra‐uterine growth‐retarded suckling piglets

This study investigated the effects of dietary supplementation with _L‐methionine (_L‐Met), _DL‐methionine (_DL‐Met) and calcium salt of the methionine hydroxyl analog (MHA‐Ca) on growth performance, intestinal morphology, antioxidant capacity and immune function in intra‐uterine growth‐retarded (IUGR) suckling piglets. Six normal birthweight (NBW) female piglets and 24 same‐sex IUGR piglets were selected at birth. Piglets were fed nutrient adequate basal diet supplemented with 0.08% _L‐alanine (NBW‐CON), 0.08% _L‐alanine (IUGR‐CON), 0.12% _L‐Met (IUGR‐LM), 0.12% _DL‐Met (IUGR‐DLM) and 0.16% MHA‐Ca (IUGR‐MHA‐Ca) from 7 to 21 days of age respectively (n = 6). The results indicated that IUGR decreased average daily milk (dry matter) intake and average daily gain and increased feed conversion ratio of suckling piglets (p < 0.05). Compared with the NBW‐CON piglets, IUGR also impaired villus morphology and reduced antioxidant capacity and immune homeostasis in the intestine of IUGR‐CON piglets (p < 0.05). Supplementation with _L‐Met enhanced jejunal villus height (VH) and villus area and ileal VH of IUGR piglets compared with IUGR‐CON piglets (p < 0.05). Similarly, _DL‐Met supplementation increased VH and the ratio of VH to crypt depth in the jejunum compared with IUGR‐CON pigs (p < 0.05). Supplementation with _L‐Met and _DL‐Met (0.12%) tended to increase reduced glutathione content and reduced glutathione: oxidized glutathione ratio and decrease protein carbonyl concentration in the jejunum of piglets when compared with the IUGR‐CON group (p < 0.10). However, supplementation with MHA‐Ca had no effect on the intestinal redox status of IUGR piglets (p > 0.10). In conclusion, supplementation with either _L‐Met or _DL‐Met has a beneficial effect on the intestinal morphology and antioxidant capacity of IUGR suckling piglets.     

妊娠期补硒对产后母鼠脏器指数、血常规参数和抗氧化能力的影响

本试验旨在研究妊娠期补充亚硒酸钠对产后母鼠脏器指数、血常规以及血清和肝脏中抗氧化能力的影响。20只健康Wistar孕鼠随机分为试验组和对照组。试验组母鼠在饮水中添加亚硒酸钠,分别为:低剂量补硒组L(0.2mg/L,n=5)、中剂量补硒组M(0.4mg/L,n=5)、高剂量补硒组H(0.6 mg/L,n=5)和对照组C(0.0 mg/L,n=5)。母鼠于分娩后第4天采样,计算脾脏和胸腺的脏器指数,检测血液生理指标,血清和肝脏中谷胱甘肽过氧化物酶(GSH-Px)、总超氧化物歧化酶(T-SOD)、总抗氧化能力(T-AOC)和丙二醛(MDA)的含量。结果显示,与对照组相比:补硒后试验组大鼠的血小板数目和体积极显著降低(P0.01),胸腺指数无显著性变化(P0.05),但脾脏指数显著升高(P0.05或P0.01);中、低剂量的亚硒酸钠可以显著性或极显著性提高产后母鼠血清和肝脏中的GSH-Px、T-AOC和T-SOD的活性(P0.05或P0.01),同时降低MDA的浓度(P0.05或P0.01)。高剂量的亚硒酸钠可以显著或极显著性提高MDA的浓度以及GSH-Px和T-AOC的活性(P0.05或P0.01),显著性降低血清中T-SOD的活性(P0.05)。妊娠期母鼠适量补充亚硒酸钠可提高产后脾脏指数及血清和肝脏中的抗氧化能力,过量补硒可以抑制抗氧化能力和减少血小板的数量,机理有待于进一步研究。     

早期数量限饲对肉用仔鸡生产性能、胴体品质的影响

试验研究了早期数量限饲对肉用仔鸡生产性能、胴体品质的影响。以320只1日龄AA+肉用仔鸡为试验动物。设4个处理组,每个处理组4个重复,每个重复20只肉仔鸡。对照组根据AA+商品代肉鸡营养标准配制。各处理肉用仔鸡1～7日龄均为自由采食,8～14日龄对照组、限饲90%组、限饲80%组和限饲70%组分别饲喂自由采食量的100%、90%、80%和70%,15日龄恢复自由采食,以后按常规饲养管理。每周末,以重复为单位全部称重。分别于42、49日龄,每个重复随机抽取4只肉用仔鸡禁食12h、称重、屠宰、测定屠体品质。试验结果表明:在第2周,限饲各组肉仔鸡的体重和日增重分别显著低于对照组(P0.05);在第3周,限饲90%组完成补偿生长,体重与对照组差异不显著(P0.05);在第4周,限饲80%和70%组完成补偿生长,体重与对照组差异不显著(P0.05);5、6、7周及2～6、2～7周各组在体重、日增重、日采食量和饲料转化率等都无显著差异(P0.05)。早期数量限饲对肉仔鸡胴体特性没有显著影响(P0.05),限饲70%～80%,具有改善饲料转化效率、胸肌率、腹脂率的作用。     

不同抗氧化剂和添加水平对肉仔鸡生产性能和抗氧化性影响的研究

为了研究不同抗氧化剂产品和添加水平对肉仔鸡生产性能和抗氧化性能的影响,本研究选用1日龄爱拔益加(AA)肉仔鸡96只,随机分为3个处理,每个处理4个重复,每个重复8只鸡。对照组(CTR)饲喂不添加任何抗氧化剂的基础日粮;SQM6组饲喂基础日粮+48mg/kgSQM6;SQMax组饲喂基础日粮+80mg/kgSQMax。记录肉仔鸡的日均采食量和平均日增重,并于28日龄时,随机在每个重复中选取1只鸡进行翅静脉采血,测定血清抗氧化指标。研究结果表明:与CTR相比,肉仔鸡日粮中添加抗氧化剂有改善饲料转化率的趋势(P>0.05);添加SQM6显著提高了血清中超氧化物歧化酶(SOD)的活性(P<0.05),并显著降低了丙二醛(MDA)的含量(P<0.05);日粮中添加SQMax显著提高了肉仔鸡血清超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性(P<0.05)。以上结果表明,肉仔鸡日粮中添加抗氧化剂SQM6和SQMax能够提高血清中超氧化物歧化酶和谷胱甘肽过氧化物酶活性,降低丙二醛含量,改善肉仔鸡机体抗氧化功能。     

发酵亚麻籽饼对肉鸡生长性能、免疫及抗氧化功能的影响

试验旨在研究发酵亚麻籽饼对肉鸡生长性能、抗氧化功能及免疫功能的影响.选取180只1日龄健康、体重约为(46.37±1.09)g的爱拔益嘉肉鸡,随机分为3组,每组6个重复,每个重复10只鸡.试验分为两个阶段:前期(1～21 d)和后期(22～42 d).对照组(CON组)饲喂玉米-豆粕型日粮,试验组分别在基础日粮中添加5...     

过量锰处理对苗期柱花草生长及抗氧化酶活性的影响

以2份柱花草基因型(TF210和TF226)为材料,分析过量锰处理对苗期柱花草生长、叶绿素浓度(SPAD)、最大光化学效率(F_v/F_m)、超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性的影响,探究柱花草中潜在的耐锰机制。结果表明,过量锰(400 μmol·L^-1)处理显著抑制了柱花草基因型TF210地上部和根系的生长、降低了其叶片SPAD和F_v/F_m值,但对TF226生长影响不明显,说明TF226为耐锰基因型,而TF210为锰敏感基因型。虽然过量锰处理增加了两个柱花草基因型组织锰含量,但TF226地上部和根部的锰含量显著低于TF210,暗示在过量锰处理下,TF226积累了较少的组织锰含量。过量锰处理显著增加了两个基因型叶片SOD活性,但仅增加了TF226叶片POD和CAT活性,并且在过量锰处理下,TF226叶片SOD、POD和CAT活性值均高于TF210。以上结果说明维持根系生长、降低组织锰含量和提高抗氧化酶活性是苗期柱花草TF226耐锰的生理机制。研究结果为探索柱花草耐锰机理及耐锰柱花草品种改良提供了理论依据及种质材料。     

Effects of alpha‐lipoic acid supplementation on growth performance,antioxidant capacity and biochemical parameters for ammonia‐exposed broilers

In order to estimate the effect of alpha‐lipoic acid (LA) supplementation on relieving ammonia stress of broilers, 180 22‐day‐old male broilers were assigned to three groups, six replicates in each group and 10 birds per replicate. The three groups were: (1) a control group without ammonia stress; (2) exposure to 70 ppm atmospheric ammonia (AM); (3) exposure to 70 ppm atmospheric ammonia and administration of 300 mg/kg LA (AM + LA). The experimental period was 3 weeks. Results showed that average daily weight gain was increased and feed conversion ratio was decreased in the AM + LA group, compared with the AM group (P < 0.05). Total superoxide dismutase and glutathione peroxidase activities in serum, and glutathione content in liver were higher in the AM + LA group than that in the AM group (P < 0.05); however, serum malondialdehyde content was decreased by LA addition (P < 0.05). Additionally, serum glutamic‐pyruvic transaminase, creatine kinase and lactate dehydrogenase activities were reduced and albumin level was increased by LA addition (P < 0.05). In conclusion, LA addition could relieve ammonia stress to restore broiler production performance to normal levels.