Feed intake, lactation performance, blood metabolites and fertility in early lactation dairy cows grazing a timothy pasture

The present study was conducted to investigate feed intake, milk yield, milk composition, blood metabolites and fertility in early lactation dairy cows grazing a timothy pasture. Fourteen multiparous Holstein cows that calved between 20 May and 19 July were used over a 3‐year period. The stocking rate was 3.6–4.3 cow/ha. Concentrates were fed separately at 9.5–11.5 kg/day per cow (dry matter basis) from 1 to 13 weeks postparturition. Herbage intake was estimated using chromium oxide as an indigestible marker. The mean contents of crude protein, total digestible nutrients and neutral detergent fiber of pasture during the 3‐year study period were 22.3%, 71.8% and 51.7%, and those of total diet were 18.9%, 77.3% and 40.3%, respectively. The mean herbage dry matter intake was 13.0 kg/day from 2 to 13 weeks postparturition during the study, total dry matter intake was 23.7 kg/day, the total digestible nutrients sufficiency rate was 105%, milk yield was 39.7 kg/day, and milk fat percentage was 3.30%. The decrease in bodyweight postparturition was slight. Urea nitrogen concentrations in serum were below 18.3 mg/dL. The mean days to first estrus and days open were 36 and 104 days, respectively. These results indicate that energy deficiency, decrease in bodyweight and fertility in early lactation barely occur when high producing dairy cows are fed enough grazing grass and suitable concentrates.     

Effects of supplementary corn silage on the feed intake and milk production of time-restricted grazing dairy cows

The effects of supplementary corn silage (CS) of either 2 or 4 kg of dry matter (DM; S + 2 and S + 4, respectively) above the energy requirement for milk production and maintenance for grazing dairy cows (S) were determined. Time‐restricted grazing was used to compare the feed intake, milk production, and nitrogen and energy use of lactating cows. The experiment was carried out on two different pastures using a 3 × 3 Latin square design for each pasture. Cows were grazed for 5 h on a rotational grazing system and were fed concentrate (1 kg per 5 kg of milk yield). Herbage intake was measured using a weighing technique. To calculate the energy and nitrogen use, whole feces and urine were collected. There was no statistical effect of the pastures. Herbage intake decreased by the addition of CS (P = 0.02). The reduction of herbage DM intake per unit consumption of supplementary CS towards the S group were 0.80 and 0.45 kg for the S + 2 and S + 4 groups, respectively. The total DM intake for the S + 4 group was higher than that for the S and S + 2 groups (P = 0.02). Milk yield did not differ among treatments, even though the total DM intake for the S + 4 group was higher than that of the S and S + 2 groups. Nitrogen and energy use did not differ with the addition of CS.     

Effect of timing and type of supplementary grain on herbage intake,nitrogen utilization and milk production in dairy cows grazed on perennial ryegrass pasture from evening to morning

The present study aimed to clarify the effect of timing and type of supplementary grain in grazing dairy cows on herbage dry matter intake (HDMI), nitrogen utilization and milk production. Eight lactating cows were allowed to graze from evening to morning during three seasonal periods (spring, summer, autumn). They were randomly allocated to four treatments (timing: pre‐ (Pre) or post‐grazing (Post), for large grain allotments consisting of 75% of daily grain offered; grain type: barley or corn) in 4 × 4 Latin square designs in each period. In the spring period, HDMI was greater for cows fed corn than those fed barley (P = 0.005), whereas cows in the Pre treatment had a similar HDMI, higher (P = 0.049) urinary purine derivative concentration and greater (P = 0.004) milk yield compared with cows in the Post treatment. In the summer and autumn periods, timing treatments did not affect HDMI, nitrogen utilization or milk production, but cows supplemented with barley had higher urinary purine derivatives concentration (P < 0.05) and milk yield (P < 0.05) compared with those supplemented with corn. The results indicate that large grain allotments immediately before evening grazing during early grazing seasons increased ruminal microbial protein synthesis and milk production without reducing HDMI regardless of grain type.     

Effect of time at pasture and herbage intake on profile of volatile organic compounds of dairy cow milk

Volatile organic compounds (VOCs) in milk were investigated as quantitative markers of herbage intake (HI) at pasture. Eight Holstein cows were fed indoors with concentrate and conserved forages (grass silage, corn silage and hay) (NG), then were divided into three treatments according to the duration of access to pasture: 4 h (G4), 8 h (G8), and 20 h (G20) per day. The HIs were 4.3, 8.6, and 13.0 kg dry matter/day for the G4, G8 and G20 treatments, respectively. Milk from cows was sampled and analyzed VOCs by the steam distillation‐extraction method and gas chromatography‐mass spectrometry (GC‐MS). From the intensity of the GC peak area, the levels of 1‐phytene (3,7,11,15‐tetramethyl‐1‐hexadecene) and 2‐phytene (3,7,11,15‐tetramethyl‐2‐hexadecene) were lowest in NG treatment and markedly increased with grazing time at pasture. With simple regression analysis on the HI to each diterpenoid, a strong correlation was found between the intensity of 1‐phytene in the milk and the HI (r = 0.807, P < 0.001). 1‐phytene content in milk could be useful as a quantitative marker of the HI of grazing cows.     

Effect of increased concentrate allotment before evening grazing on herbage intake,nitrogen utilization and rumen fermentation in dairy cows grazed on perennial ryegrass pasture

Two experiments were conducted to elucidate the effect of increased concentrate allotment before evening grazing on herbage intake, nitrogen utilization and rumen fermentation in dairy cows. In experiment 1, nine lactating cows were grazed in the morning and evening sessions (2.5 h each). The cows were allocated to treatments of three concentrate allotment levels before the evening grazing session by altering proportions to daily total offered; 25%, 50% and 75%. Daily herbage dry matter intake quadratically decreased with increasing the concentrate allotment levels (P < 0.05). Nitrogen utilization was similar among treatments. To investigate diurnal changes in rumen fermentation, a second experiment was conducted where six ruminally cannulated non‐lactating dairy cows grazed in the morning and evening sessions (3 h each) were subjected to the same treatments as experiment 1. Total volatile fatty acid concentration in the rumen linearly increased with increasing the concentrate allotment levels throughout the pre‐evening grazing session to the post‐morning grazing session (P < 0.01). The results indicate that dairy cows reduce daily herbage intake but do not alter nitrogen utilization with increasing concentrate allotment before evening grazing. © 2016 Japanese Society of Animal Science     

Relationship of rumen fill and fermentation to diurnal and seasonal variation of herbage intake in dairy cows grazed on perennial ryegrass pasture

To clarify the effect of digesta weight in the reticulorumen on diurnal and seasonal fluctuations in herbage intake, six ruminally cannulated, non‐lactating dairy cows were grazed on perennial ryegrass/white clover pasture during morning and evening sessions in spring and autumn. The digesta weight of fresh matter, dry matter (DM) and fiber in the reticulorumen at the beginning and the end of each grazing session was lower in spring than in autumn (P < 0.01). Although the digesta weight was similar between the sessions at the beginning of grazing, it was greater for the evening than for the morning at the end of grazing (P < 0.01). The large particles proportion in the digesta was lower for the morning than the evening (P < 0.01), and it was lower in spring than in autumn (P < 0.01). The concentrations of volatile fatty acids in rumen fluid were generally higher in spring compared with autumn. The herbage DM intake during the evening was greater compared with the morning in both seasons (P < 0.01). However, there was no difference in herbage DM intake between seasons. The results showed that the rumen digesta fill was not the sole factor explaining diurnal and seasonal variation of herbage intake in grazing dairy cows.     

Effect of herbage allowance and concentrate supplementation on dry matter intake, milk production and energy balance of early lactating dairy cows

The objective of this study was to investigate the effect of daily herbage allowance and concentrate supplementation level offered at approximately 40 and 80 days in milk (DIM) and the carryover effects at 120 DIM on the production performance of spring calving dairy cows. Sixty-six (30 primiparous and 36 multiparous) Holstein–Friesian dairy cows (mean calving date — 7 Feb ± 9.9 days) were randomly assigned to a 6 treatment (n = 11) grazing study. The experiment was a randomised block design with a 3 × 2 factorial arrangement of treatments (3 daily herbage allowances (DHA's; approximately 13, 16 and 19 kg DM/cow/day; > 4 cm) and 2 concentrate allowances (0 and 4 kg DM/day). Treatments were imposed from 21 February to 8 May. Following this period (subsequent 4-weeks) animals were offered a daily herbage allowance of 20 kg DM/cow/day and no concentrate. Milk production, total dry matter intake (TDMI), energy balance (EB) and blood metabolites were measured on three occasions — at approximately 40, 80 and 120 days in milk, R1, R2 and the carryover period, respectively. Cows offered a low DHA had a lower post-grazing sward height but increased sward utilisation (> 4 cm) during R1 and R2, there was no difference during the carryover period. Concentrate supplementation increased post-grazing sward height by 11% during R2 but had no effect during R1 and the carryover period. Daily herbage allowance had no effect on milk yield or composition during R1 however a low DHA tended to reduce milk yield in R2. Concentrate supplementation increased milk and solids corrected milk (SCM) yield by 4.1 and 2.8 kg/cow/day, respectively during R1 and also increased R2 milk production performance, this effect extended into the carryover period. Offering a low DHA restricted grass dry matter intake (DMI) during R1 and R2 yet concentrate supplementation significantly increased total DMI (2.3 (R1) and 3.0 (R2) kg DM/cow). Animals offered a low DHA had a significantly lower bodyweight (BW) than those offered a medium or high DHA during P1 and P2. Concentrate supplementation increased BW during P1 and P2 (+ 9 and + 14 kg/cow, respectively). There was no effect of treatment on BW during P3. There was no effect of DHA on EB in R1; during R2 animals offered a low DHA had the lowest EB. Concentrate significantly increased EB in R1 and R2 and increased plasma glucose concentration while it decreased plasma NEFA and BHB concentrations. The results of this study indicate that animals should be offered a low DHA up to 80 DIM after which DHA should be increased however animals should also be supplemented with concentrate during the early post-partum period.     

Effects of three short-term pasture allocation methods on milk production,methane emission and grazing behaviour by dairy cows

Two short-term grazing experiments were conducted with Norwegian Red cows. In Exp 1, 24 cows were randomly assigned to one of the following three pasture allocation methods (PAM): weekly pasture allowance (7RG), grazing 1/7 of 7RG each day (1SG), or grazing as 1SG but had access to grazed part of the paddock within one week (1FG). In Exp 2, 7RG was shortened to 5 days (5RG). We hypothesized that PAM will affect sward quality, quantity, intake and production differently. Pasture chemical composition changed with advancing grazing days but were not different between treatments. Pasture intake, milk yield, and methane emission were not affected by PAM. In Exp 1, 7RG cows spent less time on grazing, whereas in Exp 2, 1FG cows spent longer on grazing than others. Patterns observed in sward quality, and behavioural and physiological adaptations of cows to short-term changes in nutrient supply may explain the observed effects.     

Herbage intake and ruminal digestion of dairy cows grazed on perennial ryegrass pasture either in the morning or evening

This study aimed to clarify diurnal fluctuations of herbage intake, ruminal fermentation of herbage carbohydrates and proteins, and digesta particulate weight in the rumen of grazing dairy cows. Six ruminally cannulated, non‐lactating dairy cows were grazed on perennial ryegrass/white clover pasture either in the morning (04.00 to 08.00 hours) or the evening (16.00 to 20.00 hours). Cows grazed in the evening spent more time (P < 0.01) and consumed more herbage (P < 0.01) compared with cows grazed in the morning. Higher (P < 0.05) daily mean concentrations of total volatile fatty acid, propionate and n‐butyrate in rumen fluid were observed for cows grazed in the evening compared with cows grazed in the morning. Although cows grazed in the evening ingested more crude protein compared with cows grazed in the morning, no significant difference in NH₃‐N concentration in rumen fluid was observed between them. The ratio of purine‐derivative concentration to creatinine concentrations was higher (P < 0.01) in the urine of cows grazed in the evening than in cows grazed in the morning. These results clearly indicated that evening grazing was advantageous for dairy cows compared with morning grazing, in terms of ruminal fermentable energy intake and nitrogen utilization efficiency.     

Effect of the type of silage on milk yield,intake and rumen metabolism of dairy cows grazing swards with low herbage mass

The aim of this study was to evaluate the effect of herbage allowance (HA) and type of silage supplemented (TS) on milk yield, dry matter intake (DMI) and metabolism of dairy cows in early lactation. Thirty‐six Holstein‐Friesian dairy cows were allocated to four treatments derived from an arrangement of two HA (LHA = 17 or HHA = 25 kg of DM/cow/day) and two TS (grass (GS) or maize (MS)). Herbage allowance had no effect on DMI or milk yield. Rumen pH and NH₃‐N concentration were not affected by HA. The efficiency of microbial protein synthesis in the rumen (microbial protein (MP)) was affected by HA with 21.5 and 23.9 g microbial nitrogen per kg ruminal digestible organic matter for LHA and HHA, respectively (P < 0.05). Supplementation with MS showed higher values of milk yield by 2.4 kg/cow/day (P < 0.001), milk protein content by 0.10 % (P < 0.023) and herbage DMI by 2.2 kg/cow/day, and showed lower values for milk urea compared to GS (P < 0.001). The former results suggest that TS had a greater effect on milk yield, total feed intake and energy intake than increase in herbage allowance; however, increase in HA had greater effects on MP than TS.     

In situ provision of drinking water to grazing dairy cows improves milk production

AIMS: To determine the effect of providing water within the area grazed by dairy cows on milk yield and quality, compared to requiring cows to walk to a distant water trough, on a dairy farm in the Pampa region of Argentina during summer.

METHODS: Holstein dairy cows were allocated to two herds with similar parity, days in milk and milk production. They were grazed in one paddock that was divided in two, with a fixed water trough at one end. Cows were moved twice daily to grazing plots within the paddock. Control cows (n=66) could only access water from the fixed trough, whereas supplemented cows (n=67) also received water from a mobile trough within the grazing plot. Milk production of each cow, and water consumption of the two herds were measured daily over 62 days. Milk composition for each herd was determined weekly from Days 18 to 60 of the study, and grazing behaviour was observed between 08:00 and 16:00 hours on Days 11–15, 19–22 and 39–43.

RESULTS: Over the 62 days of the study, supplemented cows produced 1.39 (SE 0.11) L/cow/day more milk than Control cows (p=0.027). Estimated mean daily water intake was 50.4 (SE 2.1) L/cow/day for supplemented cows and 58.2 (SE 2.7) L/cow/day for Control cows (p=0.004). Percentage total solids in milk was higher for supplemented (12.5 (SE 0.06)%) than Control (12.4 (SE 0.04)%) cows (p=0.047). During the periods of behavioural observation, a higher percentage of cows in the water supplemented than the Control herd were observed in the grazing area (p=0.012).

CONCLUSIONS AND CLINICAL RELEVANCE: This preliminary study demonstrated that provision of water to dairy cows within the grazing plot was beneficial for milk production and composition, and may be associated with longer periods spent within the grazing area, during hot weather in the Pampa region of Argentina.     

Effects of ear corn silage supplementation on milk production and milk fatty acid profiles in grazing dairy farms

This study investigated the effects of substituting ear corn silage (ECS) for commercial formula feed on milk production and milk fatty acid profiles in grazing dairy farms during the summer season. A field survey was conducted on five grazing dairy farms in every summer month of 2017, 2018, and 2019. Three of the five farms substituted fresh ECS for the commercial formula feed at a ratio of 2:1 from July of each year (ECS farms). Other farms maintained the same feeding management as before (non‐ECS farms). An interview survey was conducted on each farm to calculate feed intake and milk yield per cow. Feed and milk samples were collected in each survey. Milk compositions and milk fatty acid profiles were determined. The substitution of ECS for the commercial formula feed did not affect milk yield or milk composition, but ECS farms maintained low levels of milk urea compared with non‐ECS farms (p < .01). The ECS substitution also influenced some of the milk fatty acid proportions; C16:0 and C16:1 increased, and trans‐11 C18:1, cis‐9,trans‐11 C18:2, and the sum of polyunsaturated fatty acids decreased, while these fatty acid proportions were maintained in non‐ECS farms throughout the summer season (p < .05).     

Theoretical turnover rate of the rumen liquid fraction in dairy cows and its relationship to feed intake,rumen fermentation,and milk production

Ruminant animals are able to convert plant materials (grain and the human‐indigestible portion of carbohydrates) to milk and meat. In this conversion, most of the plant materials are digested by rumen fermentation and are changed to short‐chain fatty acids, microbial cells, and methane, which is released into the atmosphere. The relationships among feed, rumen fermentation, and milk production are poorly understood. Here we report a novel indicator of characteristics of rumen fermentation, theoretical turnover rate (TTOR) of the rumen liquid fraction. The TTOR was calculated from the presumed rumen volume (PRV) which is estimated by dividing the methane yield by the methane concentration of rumen fluid. The formula for the TTOR is: TTOR = PRV/body weight^0.75. Our present analyses confirm that the TTOR as an indicator is capable of connecting feed, rumen fermentation, and milk production, because dry matter intake/TTOR showed a strong correlation with milk yield/TTOR. In addition, the TTOR may be related to ruminal pH, as we observed that the ruminal pH decreased as the TTOR increased. We propose that the TTOR is a factor characterizing rumen fermentation and a good indicator of the productivity of ruminants and dysbiosis of the rumen microbiome.     

不同微生态制剂对奶牛产奶性能和乳品质的影响

[目的]研究旨在探讨不同微生态制剂对奶牛产奶性能和乳品质的影响,[方法]将40头荷斯坦奶牛随机分成4个处理组,对照组饲喂基础日粮,微生态制剂1组、微生态制剂2组、微生态制剂3组分别在基础日粮中添加0.1%的三种微生态制剂产品。[结果]结果显示：与对照组相比,微生态制剂1组、微生态制剂2组、微生态制剂3组的平均日产奶量、乳蛋白率均显著增加（P＜0.05）,体细胞数均显著降低（P＜0.05）。与对照组相比,微生态制剂1组的平均日采食量、乳脂率均显著增加（P＜0.05）,料奶比显著降低（P＜0.05）。与微生态制剂2组相比,微生态制剂1组的平均日产奶量、乳蛋白率均显著增加（P＜0.05）。[结论]说明三种微生态制剂产品均能在一定程度上提高奶牛的产奶性能和乳品质,以微生态制剂产品1的作用效果最好。     

Effect of brewer's grain on rumen fermentation, milk production and milk composition in lactating dairy cows

Six lactating Holstein cows were divided into two groups (n = 3) and used in a double reversal trial with three periods of 14 days each to evaluate the rumen fermentation, milk production and milk composition of cows fed brewer's grain (BG). The control diets contained 14% chopped Sudangrass hay, 24% corn silage, 18% alfalfa hay cube, 34% concentrate mixture‐1 and 10% concentrate mixture‐2 (wheat bran, soybean meal and cottonseed). In the experimental diet, wet BG replaced the concentrate mixture‐2. The protozoal population, concentration of ammonia‐N and volatile fatty acids in the ruminal fluid did not differ between the control and BG diets. The molar percentage of acetic acid was significantly higher (P < 0.05) with the BG diet at 5 h after feeding. The milk yield, the percentage of protein, lactose, solids not‐fat and somatic cell counts of milk did not differ between the two diets. The percentage of milk fat tended to increase with the BG diet. The BG diet significantly increased the proportions of C18:0 and C18:1 in milk fat (P < 0.01, P < 0.05, respectively) and tended to increase that of conjugated linoleic acid.     

Effect of monensin and vitamin E on milk production and composition of lactating dairy cows

Feeding unsaturated oils to lactating dairy cows impair ruminal biohydrogenation (BH) of unsaturated fatty acids (USFA) and increase ruminal outflow of BH intermediates such as trans‐10, cis‐12 CLA that are considered to be potent inhibitors of milk fat synthesis. Supplementing lactating dairy cow’s rations containing plant origin oils with monensin and/or vitamin E may minimise the formation of trans‐10 isomers in the rumen, thereby preventing milk fat depression. Therefore, this study was conducted to evaluate the effects of monensin and vitamin E supplementation in the diets of lactating dairy cows containing whole cottonseed, as the main source of FA on feed intake, milk production and composition, milk fatty acid profile, efficiency of nitrogen (N) utilisation, efficiency of net energy (NE) utilisation and nutrients digestibilities. Four multiparous Holstein lactating dairy cows (86 ± 41 days in milk) were assigned to a balanced 4 × 4 Latin square design. Each experimental period lasted 21 days with a 14 days of treatment adaptation and a 7 days of data collection. The control diet was a total mixed ration (TMR) consisted of 430 g/kg forage and 570 g/kg of a concentrate mixture on dry matter (DM) basis. Cows were randomly assigned to one of the four dietary treatments including control diet (C), control diet supplemented with 150 mg of vitamin E/kg of DM (E), control diet supplemented with 24 mg of monensin/kg of DM (M) and control diet supplemented with 150 mg of vitamin E and 24 mg of monensin/kg of DM (EM). Dry matter intake (DMI) ranged from 19.1 to 19.5 kg/d and was similar among the dietary treatments. Dietary supplementation with vitamin E or monensin had no effect on milk production, milk fat, protein and lactose concentrations, efficiency of utilisation of nitrogen and net energy for lactation (NE_L). Digestibility of DM, organic matter (OM), crude protein (CP) and ether extract (EE) was not affected by the dietary treatments. Digestibility of neutral detergent fibre (NDF) was higher in cows fed with the M and EM diets in relation to those fed the C and E diets. The concentrations of C4:0, C6:0, C8:0, C10:0, C12:0, C14:0, C15:0, trans‐10‐16:1, cis‐9‐16:1, 17:0, 18:0, trans‐11‐18:1, cis‐9‐18:1, cis‐9, trans‐11 conjugated linoleic acid (CLA), trans‐10, cis‐12 CLA, and 18:3n‐3 FA in milk fat were not affected by the dietary supplementations. While feeding the M diet tended to decrease milk fat concentration of C16:0, the milk fat concentration of C18:2n‐6 FA tended to be increased. Dietary supplementation with vitamin E or monensin had no effect on milk fat concentrations of saturated, unsaturated, monounsaturated, polyunsaturated, short chain and long chain FA, but feeding the M diet numerically decreased milk fat concentration of medium chain fatty acids (MCFA). The results showed that vitamin E and/or monensin supplementations did not improve milk fat content and did not minimise the formation of trans‐10 FA isomers in the rumen when whole cottonseed was included in the diet as the main source of fatty acids.     

过瘤胃赖氨酸对奶牛产乳量及乳成分的影响

试验采用随机分组试验设计,选择健康的中国荷斯坦奶牛48头,按照胎次、产奶量、泌乳日期一致原则,均匀分成4组,试验各组基础日粮相同,就饲粮中添加过瘤胃赖氨酸(Lys)对乳牛产乳量及乳成分的影响进行研究。4个处理组分别为:对照组(0添加组)不添加过瘤胃赖氨酸(RPLys)和试验Ⅰ组添加10 g(/头.d)、试验Ⅱ组添加20 g(/头.d)和试验Ⅲ组添加30 g(/头.d)。试验结果显示:经过饲喂后,试验各组与对照组相比,产奶量显著提高;试验Ⅲ组和试验Ⅱ组都与对照组差异显著(P0.05),其中试验Ⅲ组与对照组差异极显著(P0.01);乳蛋白含量有所提高,其中试验Ⅲ组与对照组有显著差异(P0.05);其他乳成分指标略有提高,但差异不显著(P0.05)。     

Effects of pre-feeding of a corn silage-based supplement on the feed intake, milk production and nitrogen utilization of grazing dairy cows

Four Holstein cows were used to determine the effect of timing of the feeding of a corn silage (CS)‐based supplement on the feed intake, milk production and nitrogen utilization of grazing dairy cows. The cows were fed the supplement 2 h before grazing (pre‐grazing) or immediately after grazing (post‐grazing). Cows were grazed for 5 h per day under a rotational grazing system. There was no difference in the herbage and total feed intake between treatments. The milk protein yield for pre‐grazing tended to be higher than that for post‐grazing, whereas the milk yield did not differ between treatments. The total nitrogen intake for pre‐grazing tended to be higher than that for post‐grazing (P = 0.06). There was no difference in the urinary nitrogen output between treatments, whereas the proportion of urinary nitrogen output : total nitrogen intake for pre‐grazing tended to be lower than that for post‐grazing (P = 0.06). The milk nitrogen output and nitrogen retention for pre‐grazing tended to be higher than that for post‐grazing (milk nitrogen, P = 0.06; nitrogen retention, P = 0.05). Nitrogen utilization of grazing dairy cows was improved by feeding a CS‐based supplement before grazing.     

不同预混料及缓冲剂对奶牛泌乳性能的影响

选择6头荷斯坦奶牛研究添加预混料和复合缓冲剂对奶牛产奶量和乳脂成分的影响。试验采用3×3重复拉丁方设计,分3组处理:(Ⅰ)基础日粮+1%市售商品预混料(对照组);(Ⅱ)基础日粮+1%新型预混料;(Ⅲ)基础日粮+1%新型预混料+复合缓冲剂。结果表明:1)试验Ⅲ组的粗蛋白质、粗脂肪、中性洗涤纤维和酸性洗涤纤维的表观消化率均高于其他两组,但差异不显著(P>0.05)。2)试验Ⅲ组产奶量为23.98kg/d,比试验Ⅰ组和试验Ⅱ组分别提高5.78%和3.67%,且差异极显著(P>0.05);试验Ⅲ组的乳脂率、乳蛋白、乳糖和非脂固形物均显著高于试验Ⅰ组和试验Ⅱ组。3)试验Ⅲ组奶牛血糖含量为86.83mg/dL,极显著高于试验Ⅰ组和试验Ⅱ组;试验各组血清尿素氮含量无显著差异(P>0.05)。     

Supplementary effect of peppermint (Mentha × piperita) on dry matter intake, digestibility, ruminal fermentation and milk production in early lactating dairy cows

The aim of this research was to investigate the effect of peppermint feeding on dry matter intake, nutrient digestibility, ruminal fermentation and milk production in early lactating cows. Four Holstein cows were offered a diet with 5% dried peppermint and four Holstein cows remained on a diet without 5% of dried peppermint on a dry matter basis. The addition of peppermint to feed did not affect dry matter intake, although the eating time of feed was increased by mixing the feed with peppermint. There were no significant differences in the nutrient digestibilities between the two treatments. The ruminal ammonia and volatile fatty acids concentrations were similar in the two treatments, however, peppermint ingestion by cows led to a decrease in ruminal pH. The lowered pH value was within the stable pH condition range. No significant differences in the treatments were observed in milk production or milk composition except for the milk fat content. These results suggest that feeding peppermint to early lactating cows had little effect on their dry matter intake, nutrient digestibility, ruminal fermentation and milk production.