《新编拉汉英植物名称》 王宗训主编

本书收集具有经济价值和学术价值和学术价值或通俗常见的种子植物、蕨类植物、苔藓植物、藻类植物、真菌、地衣名称约55800条。每种植物名称有拉、汉、英三种文字对照，按拉丁文字母顺序排列。书后附有英文俗名和汉名索引。     

《新编拉汉英植物名称》

本书收集具有经济价值和学术价值和学术价值或通俗常见的种子植物、蕨类植物、苔藓植物、藻类植物、真菌、地衣名称约55800条。每种植物名称有拉、汉、英三种文字对照，按拉丁文字母顺序排列。书后附有英文俗名和汉名索引。     

《新编拉汉英植物名称》

本书收集具有经济价值和学术价值或通俗常见的种子植物、蕨类植物、苔藓植物、藻类植物、真菌、地衣名称55800条。每种植物名称有拉、汉、英三种文字对照,按拉丁文字母顺序排列。书后附有英文俗名和汉名     

《新编拉汉英植物名称》

本书收集具有经济价值和学术价值或通俗常见的种子植物、蕨类植物、苔藓植物、藻类植物、真菌、地衣名称55800条。每种植物名称有拉、汉、英,3种文字对照,按拉丁文字母顺序排列。书后附有英文俗名和汉     

《新编拉汉英植物名称》 王宗训主编

本书收集具有经济价值和学术价值或通俗常见的种子植物、蕨类植物、苔藓植物、藻类植物、真菌、地衣名称55800条。每种植物名称有拉、汉、英三种文字对照,按拉丁文字母顺序排列。书后附有英文俗名和汉名索引。     

征订《新编拉汉英植物名称》

本书收集具有经济价值和学术价值或通俗常见的种子植物、蕨类植物、苔藓植物、藻类植物、真菌、地衣名称55800条。每种植物名称有拉、汉、英三种文字对照,按拉丁文字母顺序排列。书后附有英文俗名和汉名索引。     

征订《新编拉汉英植物名称》

本书收集具有经济价值和学术价值或通俗常见的种子植物、蕨类植物、苔藓植物、藻类植物、真菌、地衣名称55800条。每种植物名称有拉、汉、英三种文字对照,按拉丁文字母顺序排列。书后附有英文俗名和汉名索引。本书可供农、林、医药、环境保护等学科的管理机构、科研单位、大学中的科技人员以及生物工程、植物检     

《新编拉汉英植物名称》王宗训主编

<正>本书收集具有经济价值和学术价值或通俗常见的种子植物、蕨类植物、苔藓植物、藻类植物、真菌、地衣名称55800条。每种植物名称有拉、汉、英三种文字对照,按拉丁文字母顺序排列。书后附有英文俗名和汉名索引。     

《新编拉汉英植物名称》

本书收集具有经济价值和学术价值或通俗常见的种子植物、蕨类植物、苔藓植物、藻类植物、真菌、地衣名称55800条。每种植物名称有拉、汉、英三种文字对照,按拉丁文字母顺序排列。书后附有英文俗名和汉名索引。本书可供农、林、医药、环境保护等学科的管理机构、科研单位、大学中的科技人员以及生物工程、植物检     

《新编拉汉英植物名称》

本书收集具有经济价值和学术价值或通俗常见的种子植物、蕨类植物、苔藓植物、藻类植物、真菌、地衣名称55800条。每种植物名称有拉、汉、英,3种文字对照,按拉丁文字母顺序排列。书后附有英文俗名和汉名索引。本书可供农、林、医药、环境保护等学科的管理机构、科研单位、大学中的科技人员以及生物工程、植物     

An investigation of branch stresses induced by arboricultural operations

This paper presents a method for assessing the safety of tree branches subject to unorthodox climbing approaches and possible falls. The method entails finite element modeling of the tree branch, experimentally or analytically determining loads associated with an ascending or falling climber, and computing stresses and safety factor along the length of the branch using dynamic structural analysis. A case study example is presented for an Ulmus americana L. branch on the campus of the University of Massachusetts, Amherst. This tree and branch were climbed using an unorthodox and controversial method during a competition in 2014. Case study models demonstrate that during an ascent, a climber’s skill has a significant effect on branch stresses, and during a simulated fall, the climber’s mass and fall distance are the key determinants of branch stresses. For loads induced during an ascent, safety factors for branches ranged between 3 and 4; for loads induced during a fall, safety factors were as low as 1.2. These values are dangerously low given the uncertainty of branch material properties. Climbers should be extremely cautious when attempting unorthodox climbing techniques.     

花椰菜种子活力和抗氧化酶活性及幼苗光合色素对NaCl胁迫的响应

以花椰菜银农70天自交系种子为材料,研究了7个NaCl胁迫浓度（0、34、68、102、136、170、204 mmol?L-1）对种子活力和抗氧化酶活性及幼苗光合色素的变化特点。结果发现：① 随着NaCl胁迫浓度的增加,种子发芽率、发芽势、发芽指数、幼苗叶绿素a/b（Chla/Chlb）和类胡萝卜素/叶绿素（Car/Chl）值呈线性降低趋势,丙二醛（MDA）含量呈线性增加趋势。② 种子超氧化物歧化酶（SOD）活性、幼苗干鲜质量以及Chla、Chlb、Chl和Car含量随NaCl胁迫浓度增加呈“钟形”抛物线变化规律,在68～136 mmol?L-1 NaCl胁迫下出现最大值|相对盐害率变化相反,呈“倒钟形”变化特点,在34 mmol?L-1 NaCl胁迫下出现最小值。③ 种子过氧化物酶（POD）和过氧化氢酶（CAT）活性均随NaCl胁迫浓度增加呈多项式变化规律,即在中度NaCl胁迫（136 mmol?L-1）下活性升高,而重度NaCl胁迫（204 mmol?L-1）下降低。综合分析表明,种子相对盐害率、抗氧化酶（SOD、POD和CAT）活性及幼苗Chla、Chlb、Chl和Car含量不仅能较好地指示NaCl胁迫对花椰菜种子活力及相关生理生化的伤害程度,并且可反映其受NaCl胁迫伤害的强度水平,但发芽率、发芽势、发芽指数、Chla/Chlb和Car/Chl值则无法指示|102～136 mmol?L-1 NaCl胁迫浓度是花椰菜种子活力及相关生理生化代谢受到伤害的盐胁迫阈值。     

逆境胁迫对‘宁玉’草莓叶片自噬小体数量及其相关基因表达的影响

为探索自噬在草莓叶片响应逆境胁迫中的作用,以‘宁玉’草莓为试材,研究了在黑暗、高盐、涝害、高温和干旱胁迫处理下,叶片自噬活动的情况以及Fa ATG5、Fa ATG6、Fa ATG7、Fa ATG8C、Fa ATG8F、Fa ATG12这6个自噬相关基因在草莓叶片中的半定量和定量表达情况。结果显示:在5种胁迫条件下,草莓叶片自噬小体的数量发生变化,都达到4个以上,其中在盐胁迫3 d时达到最多,为35个,在高温胁迫和干旱胁迫下出现先上升后下降的现象,说明高温和干旱胁迫对草莓伤害最为严重。在5种胁迫条件下,自噬相关基因表达总体呈上升趋势,并且在高温胁迫条件下,Fa ATG5、Fa ATG7、Fa ATG8F、Fa ATG12表达水平先升高后下降。在干旱胁迫条件下,Fa ATG6、Fa ATG8C、Fa ATG8F表达水平先升高后下降。Fa ATG8C和Fa ATG8F这两个基因对草莓叶片中自噬小体的形成以及应答不同胁迫中的作用更为重要。     

MicroRNA expression in hepatocytes with hydrogen peroxide-induced oxidative stress-injury and alleviating effect of mesenchymal stem cell-conditioned medium

AIM: To evaluate the changes of microRNA (miRNA) in hepatocytes during hydrogen peroxide-induced oxidative stress injury, and to observe the alleviating effect of mesenchymal stem cell-conditioned medium (MSC-CM) in this progress. METHODS: The hepatocyte oxidative stress injury model was established using hydrogen peroxide and human normal liver cell line L02. MSC-CM was prepared using centrifugation and filter. The effects of MSC-CM on hepatocyte injury were evaluated by apoptosis analysis, cell viability detection, cell cycle, and mitochondrial membrane potential (MMP). Twenty-one differentially expressed miRNAs were selected by gene chip hybridization, in which miR-143, miR-145, miR-301a and let-7a were confirmed by RT-qPCR. Bioinformatics software was utilized to predict target proteins of these miRNAs, and then the proteins were verified by Western blot.RESULTS: MSC-CM markedly attenuated hydrogen peroxide-induced oxidative stress injury by reducing apoptosis, promoting cell viability and regulating cell cycle. The expression of miR-143, miR-145, miR-301a and let-7a, indentified by RT-qPCR, increased under the condition of oxidative stress injury, while decreased after MSC-CM treatment. The expression of miR-143 predicted target proteins, HK2 and ADRB1, decreased under the hydrogen peroxide-exposure, while increased after MSC-CM treatment, which is consistent with the regulatory trend of miR-143. CONCLUSION: MSC-CM might attenuate hydrogen peroxide induced oxidative stress injury via inhibiting apoptosis and regulating some miRNA expression.     

镧对西葫芦种子酸雨胁迫缓解的剂量效应

以西葫芦种子为试材,通过种子萌发试验,研究了不同剂量镧对酸雨胁迫的缓解效应。结果表明:用8～15 mg/L的LaCl3浸种可显著改善酸雨胁迫下西葫芦种子的萌发状况。在pH 3.5时,种子的发芽率、发芽势、发芽指数和活力指数显著升高,并接近于对照;pH 3.0时也有明显改善,但达不到对照水平。同时,镧的最适剂量与酸雨胁迫的强度有关,在pH>3.5时为10mg/L;在pH≤3.5时为12 mg/L。因此,最适剂量的选择应考虑酸雨胁迫的强度,一般可用pH3.5的酸雨进行选择。     

High expression of PDX-1 in bone marrow mesenchymal stem cells mediated by superfect

AIM: To construct a eukaryotic expression vector containing pancreatic duodenal homebox-1 (PDX-1) and to elevate the expression efficiency of exogenous gene in rat bone marrow mesenchymal stem cells (MSCs). METHODS: Recombinant vector containing PDX-1 was constructed. Flow cytometry was used to identify the cell cycle of bone marrow mesenchymal stem cells (MSCs) cultured in vitro. Recombinant vector containing PDX-1 was transfected into bone marrow MSCs using superfect in medium. After being selected by G418, RT-PCR and Western blotting were used to investigate the expression of PDX-1 in MSCs. RESULTS: Restricted enzyme analysis and sequencing showed that PDX-1 gene segment was consistent with that in GenBank. Flow cytometry showed that there were about 85.9% cells at the cell cycle of G0/G1. The whole cells transfected emitted green fluorescence under flow cytometry. The efficiency of transfection was above 40%. RT-PCR and Western blotting demonstrated that there was expression of PDX-1 in transfected bone marrow MSCs. CONCLUSION: Recombinant vector containing PDX-1 was constructed successfully. Superfect mediated expression of exogenous gene in bone marrow MSCs in a high efficiency, and bone marrow MSCs containing exogenous gene are an ideal cells for gene therapy.     

苹果MdCBL家族基因表达分析及MdCBL1的功能鉴定

利用生物信息学的方法,对10个苹果MdCBLs家族蛋白的功能域进行预测,并与拟南芥AtCBLs家族的10个蛋白进行了系统进化分析,同时对苹果MdCBLs基因进行了系统的命名。利用qRT-PCR,检测了苹果MdCBLs基因在不同组织的表达及对非生物胁迫(包括盐、低温、ABA、干旱)的响应,结果表明,MdCBLs在苹果不同组织及非生物胁迫中起重要作用。另外,通过遗传转化苹果愈伤组织鉴定了MdCBL1在盐胁迫中的功能,MdCBL1过量表达明显提高了转基因愈伤组织的盐胁迫抗性。     

Influence of chronic psychological stress on periodontitis and serum isoenzyme LDH1 in rats

AIM: To investigate the role of chronic psychological stress on periodontitis and serum level of isoenzyme LDH1 in rats. METHODS: Eighty female special pathogen-free Wistar rats were randomly divided into 4 groups:(1) normal control group; (2) experimental periodontitis group: the periodontitis model was induced by wrapping 3/0 silk ligature inoculated with putative periodontopathic bacteria around the left maxillary second molar of the rats; (3) psychological stress stimulation group: the rats were treated with stress stimulation alone; (4) periodontitis model with stress stimulation group: the periodontitis models were induced by wrapping 3/0 silk ligature inoculated with putative periodontopathic bacteria around the left maxillary second molar of the rats, and then treated with stress stimulation. The rats were sacrificed at 2nd, 4th, 6th and 8th weeks after ligature. The levels of blood glucose and adrenocorticotropic hormone (ACTH) were measured as the stress markers, and the serum isoenzyme lactate dehydrogenase 1(LDH1) was used to evaluate the severity of hypoxia. The histological changes of periodontal tissues were observed under microscope with HE staining. RESULTS: The levels of blood glucose and ACTH in stress group and periodontitis with stress group were significantly higher than those in control group and experimental periodontitis group at the 2nd and 4th weeks after ligature (P<0.01). The level of serum LDH in periodontitis with stress group was significantly higher than that in control group. The level of serum LDH1 in periodontitis with stress group was significantly lower than that in control group (P<0.01). No difference of histological change in periodontal tissues was observed in control group and stress group. Severer inflammatory changes and alveolar bone destruction were observed in periodontitis with stress group than those in experimental periodontitis group. CONCLUSION: Stress stimulation is one of the inducing factors of periodontitis in rats that aggravates periodontitis by decreasing the tissue oxygenation.     

药用蒲公英低温和高温胁迫下内参基因筛选与相关基因表达分析

为研究不同温度胁迫下药用蒲公英最适内参基因与温度响应相关基因的表达,分别以低温(4℃)和高温(38℃)胁迫0、3、6、12、24和48 h共12个处理的叶片为材料,选取10个候选内参基因18S、EF1α、TUB、40S、GAPDH、β-actin、ACT11、TUA、60S和SKIP,利用RT-qPCR技术以及geNorm、NormFinder和BestKeeper软件评价其稳定性。利用筛选出的最适内参基因对药用蒲公英12个温度响应基因(AP2/ERF、Dof、ICE1、MYB、b ZIP、NTL6、HSF、Gols、HSP、NAC、XCT和WRKY)的表达水平进行定量分析。结果显示:温度胁迫下药用蒲公英最适内参基因为18S和GAPDH;药用蒲公英AP2/ERF、HSF、Glos、HSP、NAC、XCT基因在低温和高温胁迫下均为先上调后下调表达,bZIP、NTL6在温度胁迫下波动变化,Dof、ICE1、MYB在低温胁迫下先上调后下调表达,高温胁迫下持续下调表达,WRKY在高温胁迫下表达量远远高于低温胁迫。依据基因表达量推测,药用蒲公英低温和高温胁迫的响应机制存在差异,AP2/ERF、D...