MODELLING THE EFFECT OF VEGETATION COVER AND DIFFERENT TILLAGE PRACTICES ON SOIL EROSION IN VINEYARDS: A CASE STUDY IN VRÁBLE (SLOVAKIA) USING WATEM/SEDEM

Vineyards represent one of the most erosion‐prone types of cultivated land. Because of this, cultivation practices are very important in reducing the soil erosion risk in vineyard regions. The aim of this paper was to assess the impact of various management practices on soil loss in vineyards. Effects of tillage, hoeing, rotavating and grass cover were evaluated in small vineyards located in southwestern Slovakia in the Vráble viticultural district. Erosion and deposition rates were estimated using the levelling method. This method is based on an evaluation of variability of the soil surface against vineyard poles measured between the year of pole insertion and the year of measurement. On the basis of the measured data, a WATEM/SEDEM distributed soil erosion model was calibrated, and the total soil loss from the vineyards under different management conditions was estimated. The model shows rather good performance in modelling soil erosion, but at the same time, it shows lower reliability in modelling soil deposition. Downslope tilled vineyards were the most eroded; the erosion in rotavated vineyards is somewhat reduced. The most protective tillage system is hoeing. Considerably lower soil loss was estimated with the use of vegetation cover between vine rows, which is in accordance with agro‐environmental schemes supporting use of grass cover as an erosion prevention measure in vineyards. Copyright © 2012 John Wiley & Sons, Ltd.     

Specific response of fungal and bacterial residues to one-season tillage and repeated slurry application in a permanent grassland soil

The dynamics of fungal and bacterial residues to a one-season tillage event in combination with manure application in a grassland soil are unknown. The objectives of this study were (1) to assess the effects of one-season tillage event in two field trials on the stocks of microbial biomass, fungal biomass, microbial residues, soil organic C (SOC) and total N in comparison with permanent grassland; (2) to determine the effects of repeated manure application to restore negative tillage effects on soil microbial biomass and residues. One trial was started 2 years before sampling and the other 5 years before sampling. Mouldboard ploughing decreased the stocks of SOC, total N, microbial biomass C, and microbial residues (muramic acid and glucosamine), but increased those of the fungal biomarker ergosterol in both trials. Slurry application increased stocks of SOC and total N only in the short-term, whereas the stocks of microbial biomass C, ergosterol and microbial residues were generally increased in both trials, especially in combination with tillage. The ergosterol to microbial biomass C ratio was increased by tillage, and decreased by slurry application in both trials. The fungal C to bacterial C ratio was generally decreased by these two treatments. The metabolic quotient qCO₂ showed a significant negative linear relationship with the microbial biomass C to SOC ratio and a significant positive relationship with the soil C/N ratio. The ergosterol to microbial biomass C ratio revealed a significant positive linear relationship with the fungal C to bacterial C ratio, but a negative one with the SOC content. Our results suggest that slurry application in grassland soil may promote SOC storage without increasing the role of saprotrophic fungi in soil organic matter dynamics relative to that of bacteria.     

耕作方式对双季稻田土壤有机碳储量的影响

Tillage practices can potentially afect soil organic carbon (SOC) accumulation in agricultural soils. A 4-year experiment was conducted to identify the influence of tillage practices on SOC sequestration in a double-cropped rice (Oryza sativa L.) field in Hunan Province of China. Three tillage treatments, no-till (NT), conventional plow tillage(PT), and rotary tillage(RT), were laid in a randomized complete block design. Concentrations of SOC and bulk density(BD) of the 0-80 cm soil layer were measured, and SOC stocks of the 0-20 and 0-80 cm soil layers were calculated on an equivalent soil mass(ESM) basis and fixed depth (FD) basis.Soil carbon budget(SCB) under diferent tillage systems were assessed on the basis of emissions of methane(CH4) and CO2 and the amount of carbon (C) removed by the rice harvest. After four years of experiment, the NT treatment sequestrated more SOC than the other treatments. The SOC stocks in the 0-80 cm layer under NT (on an ESM basis) was as high as 129.32 Mg C ha 1,significantly higher than those under PT and RT (P < 0.05). The order of SOC stocks in the 0-80 cm soil layer was NT > PT > RT,and the same order was observed for SCB; however, in the 0-20 cm soil layer, the RT treatment had a higher SOC stock than the PT treatment. Therefore, when comparing SOC stocks, only considering the top 20 cm of soil would lead to an incomplete evaluation for the tillage-induced efects on SOC stocks and SOC sequestrated in the subsoil layers should also be taken into consideration. The estimation of SOC stocks using the ESM instead of FD method would better reflect the actual changes in SOC stocks in the paddy filed, as the FD method amplified the tillage efects on SOC stocks. This study also indicated that NT plus straw retention on the soil surface was a viable option to increase SOC stocks in paddy soils.     

Effect of cropland management and slope position on soil organic carbon pool at the North Appalachian Experimental Watersheds

Soil organic matter is strongly related to soil type, landscape morphology, and soil and crop management practices. Therefore, long-term (15–36-years) effects of six cropland management systems on soil organic carbon (SOC) pool in 0–30 cm depth were studied for the period of 1939–1999 at the North Appalachian Experimental Watersheds (<3 ha, Dystric Cambisol, Haplic Luvisol, and Haplic Alisol) near Coshocton, OH, USA. Six management treatments were: (1) no tillage continuous corn with NPK (NC); (2) no tillage continuous corn with NPK and manure (NTC-M); (3) no tillage corn–soybean rotation (NTR); (4) chisel tillage corn–soybean rotation (CTR); (5) moldboard tillage with corn–wheat–meadow–meadow rotation with improved practices (MTR-I); (6) moldboard tillage with corn–wheat–meadow–meadow rotation with prevalent practices (MTR-P). The SOC pool ranged from 24.5 Mg ha⁻¹ in the 32-years moldboard tillage corn (Zea mays L.)–wheat (Triticum aestivum L.)–meadow–meadow rotation with straight row farming and annual application of fertilizer (N:P:K=5:9:17) of 56–112 kg ha⁻¹ and cattle (Bos taurus) manure of 9 Mg ha⁻¹ as the prevalent system (MTR-P) to 65.5 Mg ha⁻¹ in the 36-years no tillage continuous corn with contour row farming and annual application of 170–225 kg N ha⁻¹ and appropriate amounts of P and K, and 6–11 Mg ha⁻¹ of cattle manure as the improved system (NTC-M). The difference in SOC pool among management systems ranged from 2.4 to 41 Mg ha⁻¹ and was greater than 25 Mg ha⁻¹ between NTC-M and the other five management systems. The difference in the SOC pool of NTC-M and that of no tillage continuous corn (NTC) were 16–21 Mg ha⁻¹ higher at the lower slope position than at the middle and upper slope positions. The effect of slope positions on SOC pools of the other management systems was significantly less (<5 Mg ha⁻¹). The effects of manure application, tillage, crop rotation, fertilizer rate, and soil and water conservation farming on SOC pool were accumulative. The NTC-M treatment with application of NPK fertilizer, lime, and cattle manure is an effective cropland management system for SOC sequestration.     

Microbial properties and nitrogen contents of arable soils under different tillage regimes

Attention is being paid to the use of different tillage regimes as a means of retaining soil organic carbon (SOC) and sequestering more SOC. Alongside earlier measurements of total SOC stocks under different tillage regimes, we have examined the distribution of nitrogen (N), microbial activity and the structure of the soil bacterial community from differently tilled plots under continuous barley. The plots were established 5 yr before sampling and have been maintained annually under conventional tillage (CT; moldboard ploughing to 20 cm and disking), deep ploughing (DP; ploughing to 40 cm and disking), minimum tillage (MT; disking to 7 cm) or zero tillage (ZT). Our earlier work showed there was no difference in SOC contents down to 60‐cm depth between the treatments, but now we report that there were significant differences in the total N and active microbial biomass (substrate‐induced respiration) contents of the same soils. The N contents of the CT, DP and MT treatments were not significantly different, but the ZT contained significantly more N, indicating either greater N retention under the ZT treatment or preferential loss from the more intensively tilled treatments, or a combination of both. The microbial biomass content was greater for the CT and DP treatments than for the MT and ZT treatments, indicating greater sensitivity to treatment effects of the microbial biomass pool than the total C pool, consistent with its more dynamic nature. Terminal restriction fragment length polymorphism (T‐RFLP) analyses of the soil bacteria DNA (a method of assessing the bacterial community structure) enabled the samples to be distinguished both according to SOC content, which is to be expected, and to tillage regime with the greatest differences in community structure occurring in the ZT treatment and the least in DP and CT treatments, reflecting the degree of homogenization or disturbance resulting from tillage.     

Changes in soil microbial community composition in response to fertilization of paddy soils in subtropical China

Repeated fertilizer applications to cultivated soils may alter the composition and activities of microbial communities in terrestrial agro-ecosystems. In this study, we investigated the effects of different long term fertilization practices (control (CK), three levels of mineral fertilizer (N₁P₁K₁, N₂P₂K₂, and N₃P₃K₃), and organic manure (OM)) on soil environmental variables and microbial communities by using phospholipid fatty acid (PLFA) biomarkers analysis in subtropical China. Study showed that OM treatment led to increases in soil organic carbon (SOC), total nitrogen (TN) and total phosphorus (TP) contents, while the mineral fertilizer treatment led to increases in dissolved organic carbon (DOC) content. Changes in soil microbial communities (eg. bacteria, actinomycetes) were more noticeable in soils subjected to organic manure applications than in the control soils or those treated with mineral fertilizer applications. Fungal PLFA biomarkers responded differently from the other PLFA groups, the numerical values of fungal PLFA biomarkers were similar for all the OM and mineral fertilizer treatments. PCA analysis showed that the relative abundance of most PLFA biomarkers increased in response to OM treatment, and that increased application rates of the mineral fertilizer changed the composition of one small fungal PLFA biomarker group (namely 18:3ω6c and 16:1ω5c). Further, from the range of soil environmental factors that we examined, SOC, TN and TP were the key determinants affecting soil microbial community. Our results suggest that organic manure should be recommended to improve soil microbial activity in subtropical agricultural ecosystems, while increasing mineral fertilizer applications alone will not increase microbial growth in paddy soils.     

Soil microbial community composition as affected by restoration practices in California grassland

Agricultural practices have strong impacts on soil microbes including both the indices related to biomass and activity as well as those related to community composition. In a grassland restoration project in California, where native perennial bunchgrasses were introduced into non-native annual grassland after a period of intensive tillage, weeding, and herbicide use to reduce the annual seed bank, microbial community composition was investigated. Three treatments were compared: annual grassland, bare soil fallow, and restored perennial grassland. Soil profiles down to 80 cm in depth were investigated in four separate layers (0-15, 15-30, 30-60, and 60-80 cm) using both phospholipid ester-linked fatty acid (PLFAs) and ergosterol as biomarkers in addition to microbial biomass C by fumigation extraction. PLFA fingerprinting showed much stronger differences between the tilled bare fallow treatment vs. grasslands, compared to fewer differences between restored perennial grassland and annual grassland. The presence or absence of plants over several years clearly distinguished microbial communities. Microbial communities in lower soil layers were little affected by management practices. Regardless of treatment, soil depth caused a strong gradient of changing habitat conditions, which was reflected in Canonical Correspondence Analysis of PLFAs. Fungal organisms were associated with the presence of plants and/or litter since the total amount and the relative proportion of fungal markers were reduced in the tilled bare fallow and in lower layers of the grassland treatments. Total PLFA and soil microbial biomass were highly correlated, and fungal PLFA biomarkers showed strong correlations to ergosterol content. In conclusion, microbial communities are resilient to the grassland restoration process, but do not reflect the change in plant species composition that occurred after planting native bunchgrasses.     

Soil morphology,depth and grapevine root frequency influence microbial communities in a Pinot noir vineyard

The composition of microbial communities responds to soil resource availability, and has been shown to vary with increasing depth in the soil profile. Soil microorganisms partly rely on root-derived carbon (C) for growth and activity. Roots in woody perennial systems like vineyards have a deeper vertical distribution than grasslands and annual agriculture. Thus, we hypothesized that vineyard soil microbial communities along a vertical soil profile would differ from those observed in grassland and annual agricultural systems. In a Pinot noir vineyard, soil pits were excavated to ca. 1.6–2.5 m, and microbial community composition in ‘bulk’ (i.e., no roots) and ‘root’ (i.e., roots present) soil was described by phospholipid ester-linked fatty acids (PLFA). Utilization of soil taxonomy aided in understanding relationships between soil microbial communities, soil resources and other physical and chemical characteristics. Soil microbial communities in the Ap horizon were similar to each other, but greater variation in microbial communities was observed among the lower horizons. Soil resources (i.e., total PLFA, or labile C, soil C and nitrogen, and exchangeable potassium) were enriched in the surface horizons and significantly explained the distribution of soil microbial communities with depth. Soil chemical properties represented the secondary gradient explaining the differentiation between microbial communities in the B-horizons from the C-horizons. Relative abundance of Gram-positive bacteria and actinomycetes did not vary with depth, but were enriched in ‘root’ vs. ‘bulk’ soils. Fungal biomarkers increased with increasing depth in ‘root’ soils, differing from previous studies in grasslands and annual agricultural systems. This was dependent on the deep distribution of roots in the vineyard soil profile, suggesting that the distinct pattern in PLFA biomarkers may have been strongly affected by C derived from the grapevine roots. Gram-negative bacteria did not increase in concert with fungal abundance, suggesting that acidic pHs in lower soil horizons may have discouraged their growth. These results emphasize the importance of considering soil morphology and associated soil characteristics when investigating effects of depth and roots on soil microorganisms, and suggest that vineyard management practices and deep grapevine root distribution combine to cultivate a unique microbial community in these soil profiles.     

A simplified modelling approach for quantifying tillage effects on soil carbon stocks

Soil tillage has been shown to affect long‐term changes in soil organic carbon (SOC) content in a number of field experiments. This paper presents a simplified approach for including effects of tillage in models of soil C turnover in the tilled‐soil layer. We used an existing soil organic matter (SOM) model (CN‐SIM) with standard SOC data for a homogeneous tilled layer from four long‐term field experiments with conventionally tilled (CT) and no‐till (NT) treatments. The SOM model was tested on data from long‐term (>10 years) field trials differing in climatic conditions, soil properties, residue management and crop rotations in Australia, Brazil, the USA and Switzerland. The C input for the treatments was estimated using data on crop rotation and residue management. The SOM model was applied for both CT and NT trials without recalibration, but incorporated a ‘tillage factor’ (TF) to scale all decomposition and maintenance parameters in the model. An initial value of TF = 0.57 (parameter uncertainty, PU = 0.15) for NT (with TF set to 1.0 for CT) was used on the basis of a previous study with observations of soil CO₂ respiration. The simulated and observed changes in SOC were then compared using slopes of linear regressions of SOC changes over time. Results showed that the SOM model captured observed changes in SOC content from differences in rotations, N application and crop residue management for conventional tillage. On the basis of SOC change data a mean TF of 0.48 (standard deviation, SD = 0.12) was estimated for NT. The results indicate that (i) the estimated uncertainty of tillage effects on SOC turnover may be smaller than previously thought and (ii) simple scaling of SOM model parameters may be sufficient to capture the effects of soil tillage on SOM turnover in the tilled layer. Scenario analyses showed that the average extra C input needed to compensate for soil tillage was 762 (SD = 351) kg C ha⁻¹ year⁻¹. Climatic conditions (temperature and precipitation) also affected how much extra C was needed, with substantially larger inputs being required for wetter and warmer climates.     

Effects of short-term conservation management practices on soil organic carbon fractions and microbial community composition under a rice-wheat rotation system

The objective of this study was to investigate the effects of short-term (less than 2 years) conservation managements [no-tillage (NT) and crop residue returning] on top soil (0–5 cm) microbial community composition and soil organic C (SOC) fractions under a rice-wheat rotation at Junchuan town of Hubei Province, China. Treatments were established following a split-plot design of a randomized complete block with tillage practices [conventional tillage (CT) and NT] as the main plot and residue returning level [no residue returning (0) and all residues returned to fields from the preceding crop (S, 2,146 kg C ha^?1)] as the subplots. The four treatments were CT with or without residue returning (CT0 and CTS) and NT with or without residue returning (NT0 and NTS). The abundances of microbial groups [total FLFAs, fungal biomass, bacterial biomass, fungal biomass/bacterial biomass (F/B), monounsaturated fatty acids/saturated fatty acids (MUFA/STFA), and microbial stress] were determined by phospholipid fatty acid (PLFA) analysis of soil. The ratio of MUFA/STFA reflects aeration of soil and greater MUFA/STFA means better aeration condition of soil. Moreover, the microbial stress, the ratio of cy19:0 to 18:1ω7, was regarded as an indicator of physiological or nutritional stress of microbial community. PLFA profiles were dominated by the fatty acids iC15:0 (9.8 %), C16:0 (16.5 %), 10Me17:0 (9.9 %), and Cyc19:0 (8.3 %), together accounting for 44.6 % of the total PLFAs. Compared with CT, NT significantly increased microbial biomass C (MBC) by 20.0 % but did not affect concentrations of total organic C (TOC), dissolved organic C (DOC), easily oxidizable C (EOC), and SOC of aggregates. Residue returning significantly increased MBC by 18.3 % and SOC content of 2–1-mm aggregate by 9.4 %. NT significantly increased total PLFAs by 9.8 % and fungal biomass by 40.8 % but decreased MUFA/STFA by 15.5 %. Residue returning significantly enhanced total PLFAs, bacterial biomass, fungal biomass, F/B, and MUFA/STFA by 31.1, 36.0, 95.9, 42.5, and 58.8 %, respectively, but decreased microbial stress by 45.9 %. Multivariate analysis (redundancy analysis and partial correlation analysis) indicated that SOC of 2–1-mm aggregate was related to changes in the composition of soil microbial groups, suggesting that SOC of 2–1-mm aggregate was sensitive to changes in soil microbial community composition affected by short-term conservation management practices in our study.     

Short-term changes in nitrogen availability, gas fluxes (CO2, NO, N2O) and microbial biomass after tillage during pasture re-establishment in Rondônia, Brazil

Anthropogenic conversion of primary forest to pasture for cattle production is still frequent in the Amazon Basin. Practices adopted by ranchers to restore productivity to degraded pasture have the potential to alter soil N availability and N gas losses from soils. We examined short-term (35 days) effects of tillage prior to pasture re-establishment on soil N availability, CO₂, NO and N₂O fluxes and microbial biomass C and N under degraded pasture at Nova Vida ranch, Rondônia, Brazilian Amazon. We collected soil samples and measured gas fluxes in tilled and control (non tilled pasture) 12 times at equally spaced intervals during October 2001 to quantify the effect of tillage. Maximum soil NH₄⁺ and NO₃⁻ pools were 13.2 and 6.3 kg N ha⁻¹ respectively after tillage compared to 0.24 and 6.3 kg N ha⁻¹ in the control. Carbon dioxide flux ranged from 118 to 181 mg C–CO₂ m² h⁻¹ in the control (non-tilled) and from 110 to 235 mg C–CO₂ m² h⁻¹ when tilled. Microbial biomass C varied from 365 to 461 μg g⁻¹ in the control and from 248 to 535 μg g⁻¹ when tilled. The values for N₂O fluxes ranged from 1.22 to 96.9 μg N m⁻² h⁻¹ in the tilled plots with a maximum 3 days after the second tilling. Variability in NO flux in the control and when tilled was consistent with previous measures of NO emissions from pasture at Nova Vida. When tilled, the NO/N₂O ratio remained <1 after the first tilling suggesting that denitrification dominated N cycling. The effects of tilling on microbial parameters were less clear, except for a decrease in qCO₂ and an increase in microbial biomass C/N immediately after tilling. Our results suggest that restoration of degraded pastures with tillage will lead to less C matter, at least initially. Further long-term research is needed.     

Effects of compost input and tillage intensity on soil microbial biomass and activity under Mediterranean conditions

Organic amendment and tillage reduction are two common practices to contrast soil organic matter decline, thus promoting sustainable cropping and carbon sequestration. In a horticultural land use system under Mediterranean climate, we evaluated the 9-year effects of two compost inputs (15 and 30 t ha⁻¹ y⁻¹, low and high input, respectively) and two tillage intensities (intensive and reduced) on soil macronutrients concentration, microbial biomass and activity. Total organic C, total N and P_Olsen were smaller in plots amended at low input, whilst intensive tillage decreased them at both compost inputs. These decreases in intensively tilled plots was ascribed to the disruption of soil aggregates, with consequent microbial degradation of the physically protected organic matter by oxidative processes. On the contrary, reduced tillage increased the extractable C, likely due to a higher protection of the most labile soil C fraction from the mineralization. Similarly, microbial biomass C and N increased following both doubling compost input and reducing tillage intensity, with a greater effect by the first factor. The higher values of cumulative 10-day basal and 20-h glucose-induced respiration, and metabolic quotient in intensively tilled plots suggested that high tillage favoured soil aggregate disruption and C accessibility. This was also confirmed by higher values of dehydrogenase activity/total organic C in those plots. Intensive tillage caused a higher soil aeration and organic substrates accessibility to microflora, thus undoing the fertility benefits provided by the high compost input. However, also a low compost input coupled to reduced tillage seemed to accomplish soil sustainability needs.     

Soil Quality Indicators as Affected by Shallow Tillage in a Vineyard Grown in a Semiarid Mediterranean Environment

Within the Mediterranean basin, soil tillage enhances the mineralisation of soil organic matter. We assessed the short‐term impact of shallow tillage [field cultivator (FC), rotary tiller (RT) and spading machine (SM)] on some soil quality indicators [bulk density, water‐stable aggregates, total and labile organic C pools (microbial biomass and extractable organic C), soil respiration and related eco‐physiological indexes] in a Sicilian vineyard. Also no tillage was included. We hypothesized that (i) RT and FC worsened soil quality indicators more than SM, and (ii) within the same tillage system, labile C pools, soil respiration and eco‐physiological indexes will respond more efficiently than chemical and physical soil properties since the tillage starts. The experiment started at March 2009, and each tillage type was applied three times per year (March or April, May and June), with soil tilled up to 15‐cm depth. Soil was sampled (0–15 and 15–30‐cm depth) in March 2009, April 2010, May 2012 and June 2014. SM was very effective in preserving soil organic matter pool and in improving any monitored soil quality indicator, similarly to no tillage. By contrast, RT was the most deleterious machine as it worsened most investigated indicators. Such deleterious effects were due to drastic disruption of soil aggregates and consequent exposition of protected soil organic matter to further microbial mineralization. Labile organic C pools and microbial quotients were the most responsive soil parameters for assessing the impact of shallow tillage on soil quality, even in the short term (<5 years). Copyright © 2016 John Wiley & Sons, Ltd.     

Response of soil structure and hydraulic conductivity to reduced tillage and animal manure in a temperate loamy soil

We studied the combined effects of reduced tillage and animal manure on soil structure and hydraulic conductivity (K) in the 2–10 and 12–20 cm layers in a loamy soil. The study was performed at the end of a 7‐yr field trial and included three tillage treatments (mouldboard ploughing until 25 cm depth: MP, shallow tillage until 12 cm depth: ST, no‐till: NT) and two fertilizer application treatments (mineral or poultry manure). Soil structure was assessed through bulk density (ρ_b), micromorphological and macropore‐space characteristics. K was measured in situ at ?0.6, ?0.2 and ?0.05 kPa. Untilled layers had a vermicular microstructure resulting from earthworm activity, whereas tilled layers displayed a mixture of crumb and channel microstructures. Untilled layers had the highest ρ_b and twice as much lower total macroporosity area (pores > 240 μm in equivalent diameter) than tilled layers, reflected by the smallest area of macropores 310–2000 μm in diameter and the smallest area of large complex macropores. K under untilled layers was 12–62% lower than that under tilled layers, but differences were statistically significant only at ?0.05 kPa in the 2–10 cm. No significant interaction between tillage and nutrient application treatments was detected for all properties. Compared with mineral fertilizer, poultry manure resulted in a similar ρ_b but 20% greater total macroporosity area and 30% higher K at ?0.2 kPa. Overall, the sensitivity of soil structure and K to poultry manure were relatively small compared with tillage. We suggest that cultivation practices other than animal manure application are needed to improve physical properties under reduced tillage.     

Abundance, production and stabilization of microbial biomass under conventional and reduced tillage

Soil tillage practices affect the soil microbial community in various ways, with possible consequences for nitrogen (N) losses, plant growth and soil organic carbon (C) sequestration. As microbes affect soil organic matter (SOM) dynamics largely through their activity, their impact may not be deduced from biomass measurements alone. Moreover, residual microbial tissue is thought to facilitate SOM stabilization, and to provide a long term integrated measure of effects on the microorganisms. In this study, we therefore compared the effect of reduced (RT) and conventional tillage (CT) on the biomass, growth rate and residues of the major microbial decomposer groups fungi and bacteria. Soil samples were collected at two depths (0-5 cm and 5-20 cm) from plots in an Irish winter wheat field that were exposed to either conventional or shallow non-inversion tillage for 7 growing seasons. Total soil fungal and bacterial biomasses were estimated using epifluorescence microscopy. To separate between biomass of saprophytic fungi and arbuscular mycorrhizae, samples were analyzed for ergosterol and phospholipid fatty acid (PLFA) biomarkers. Growth rates of saprophytic fungi were determined by [¹⁴C]acetate-in-ergosterol incorporation, whereas bacterial growth rates were determined by the incorporation of ³H-leucine in bacterial proteins. Finally, soil contents of fungal and bacterial residues were estimated by quantifying microbial derived amino sugars. Reduced tillage increased the total biomass of both bacteria and fungi in the 0-5 cm soil layer to a similar extent. Both ergosterol and PLFA analyses indicated that RT increased biomass of saprophytic fungi in the 0-5 cm soil layer. In contrast, RT increased the biomass of arbuscular mycorrhizae as well as its contribution to the total fungal biomass across the whole plough layer. Growth rates of both saprotrophic fungi and bacteria on the other hand were not affected by soil tillage, possibly indicating a decreased turnover rate of soil microbial biomass under RT. Moreover, RT did not affect the proportion of microbial residues that were derived from fungi. In summary, our results suggest that RT can promote soil C storage without increasing the role of saprophytic fungi in SOM dynamics relative to that of bacteria.     

Simulation and 137Cs tracer show tillage erosion translocating soil organic carbon,phosphorus, and potassium

Research on how tillage‐induced soil redistribution affects soil properties is limited for complex slopes in nonmechanized agricultural areas. The objectives of this study are (1) to examine the vertical redistribution of soil organic C (SOC), extractable P and K induced by tillage on a complex slope, (2) to assess the effects of tillage erosion on soil profile properties, and (3) to elucidate the variations in soil properties induced by both vertical mixture and downslope transport of soil within the landscape. Simulated tillage was conducted in the Yangtze Three Gorges Reservoir Area, China. The ¹³⁷Cs data showed that intense tillage caused the soil vertical mixture and downslope transport. The redistribution of ¹³⁷Cs and soil constituents varied with the number of tillage passes and location in relation to curvature. SOC was completely depleted with the disappearance of soil profiles at the summit position, while SOC concentrations decreased by 26% for the till layer and increased by 29% for the sublayer at the toeslope position for the 15‐tillage operation, as compared with those of pretillage. The vertical redistribution of extractable P and K followed a similar pattern to that of SOC. The gap and variation in soil constituents between the till layer and sublayer declined after tillage, suggesting that the mixing effect of tillage attenuates the variability of soil properties in the vertical direction. Net loss and gain of soil constituents occurred at the summit and toeslope positions, respectively, suggesting that the downslope transport of soil induced by tillage accentuates the variability of soil properties in the lateral direction.     

Modelling the Effect of Land Management Changes on Soil Organic Carbon Stocks in a Mediterranean Cultivated Field

Land management in agricultural lands has important effects on soil organic carbon (SOC) dynamics. These effects are particularly relevant in the Mediterranean region, where soils are fragile and prone to erosion. Increasing interest of modelling to simulate SOC dynamics and the significance of soil erosion on SOC redistribution have been linked to the development of some recent models. In this study, the SPEROS‐C model was implemented in a 1.6‐ha cereal field for a 150‐year period covering 100 years of minimum tillage by animal traction, 35 years of conventional tillage followed by 15 years of reduced tillage by chisel to evaluate the effects of changes in land management on SOC stocks and lateral carbon fluxes in a Mediterranean agroecosystem. The spatial patterns of measured and simulated SOC stocks were in good agreement, and their spatial variability appeared to be closely linked to soil redistribution. Changes in the magnitude of lateral SOC fluxes differed between land management showing that during the conventional tillage period the carbon losses is slightly higher (0.06 g C m⁻² yr⁻¹) compared to the period of reduced till using chisel (0.04 g C m⁻² yr⁻¹). Although the results showed that the SPEROS‐C model is a potential tool to evaluate erosion induced carbon fluxes and assess the relative contribution of different land management on SOC stocks in Mediterranean agroecosystems, the model was not able to fully represent the observed SOC stocks. Further research (e.g. input parameters) and model development will be needed to achieve more accurate results. Copyright © 2016 John Wiley & Sons, Ltd.     

Seasonal variation of microbial biomass, activity, and community structure in soil under different tillage and phosphorus management practices

No-tillage systems contribute to physical, chemical and biological changes in the soil. The effects of different tillage practices and phosphorus (P) fertilization on soil microbial biomass, activity, and community structure were studied during the maize growing season in a maize–soybean rotation established for 18 years in eastern Canada. Soil samples were collected at two depths (0–10 and 10–20 cm) under mouldboard plow (MP) and no-till (NT) management and fertilized with 0, 17.5, and 35 kg P ha^?1. Results show that the duration of the growing season had a greater effect on soil microbiota properties than soil tillage or P fertilization at both soil depths. Seasonal fluctuations in soil microbial biomass carbon (SMB-C) and nitrogen (SMB-N), in dehydrogenase and alkaline phosphomonoesterase activities, and in total phospholipids fatty acid (PLFA) level, were greater under NT than MP management. The PLFA biomarkers separated treatments primarily by sampling date and secondly by tillage management, but were not significantly affected by P fertilization. The abundance of arbuscular mycorrhizal fungi (AMF; C16:1ω5) and fungi (C18:2ω6,9) was lower under NT than MP at the 10–20-cm soil depth in July. Phosphorus fertilization increased soil microbial biomass phosphorus (SMB-P) and Mehlich-3 extractable P, but had a limited impact on the other soil properties. In conclusion, soil environmental factors and tillage had a greater effect on microorganisms (biomass and activity) and community structure than P fertilization.     

Microbial biomass in soils of Russia under long-term management practices

 Non-tilled and tilled plots on a spodosol (C_org 0.65–1.70%; pH 4.1–4.5) and a mollisol (C_org 3.02–3.13%, pH 4.9–5.3), located in the European region of Russia, were investigated to determine variances in soil microbial biomass and microbial community composition. Continuous, long-term management practices, including tillage and treatment with inorganic fertilizers or manure, were used on the spodosol (39 years) and mollisol (22 years). Total microbial biomass (C_mic), estimated by the substrate-induced respiration (SIR) method, and total fungal hyphae length (membrane filter technique) were determined seasonally over a 3-year period. Long-term soil management practices (primarily tillage and fertilizer application) led to decreases in total microbial biomass (80–85% lower in spodosol and 20–55% lower in mollisol), decreases in the contribution of C_mic to C_org (2.3- to 3.5-fold lower in spodosol and 1.2- to 2.3-fold lower in mollisol), and 50–87% decreases in total fungal hyphae length compared to non-tilled control plots. The contribution of fungi to total SIR in virgin mollisol and fallow spodosol plots was approximately 30%. However, the contribution of fungi to SIR was approximately two times greater in tilled spodosol plots compared to a fallow plot. In contrast, the contribution of fungi to SIR in tilled plots of mollisol was less (1.4–4.7 times) than for a virgin plot. In summary, long-term soil management practices such as tillage and treatment with organic or inorganic fertilizers are important determinants of soil microbial biomass and the contribution of fungi to total SIR. Received: 28 April 1998     

干旱热带地区土地利用变化对土壤微生物群落组成及有机碳含量的影响

Restoration of forests poses a major challenge globally,particularly in the tropics,as the forests in these regions are more vulnerable to land-use change.We studied land-use change from natural forest (NF) to degraded forest (DF),and subsequently to either Jatropha curcas plantation (JP) or agroecosystem (AG),in the dry tropics of Uttar Pradesh,India,with respect to its impacts on soil microbial community composition as indicated by phospholipid fatty acid (PLFA) biomarkers and soil organic carbon (SOC) content.The trend of bacterial PLFAs across all land-use types was in the order:NF ＞ JP ＞ DF＞ AG.In NF,there was dominance of gram-negative bacterial (G-) PLFAs over the corresponding gram-positive bacterial (G+) PLFAs.The levels of G-PLFAs in AG and JP differed significantly from those in DF,whereas those of G+ PLFAs were relatively similar in these three land-use types.Fungal PLFAs,however,followed a different trend:NF ＞ JP ＞ DF =AG.Total PLFAs,fungal/bacterial (F/B) PLFA ratio,and SOC content followed trends similar to that of bacterial PLFAs.Across all land-use types,there were strong positive relationships between SOC content and G-,bacterial,fungal,and total microbial PLFAs and F/B PLFA ratio.Compared with bacterial PLFAs,fungal PLFAs appeared to be more responsive to land-use change.The F/B PLFA ratio,fungal PLFAs,and bacterial PLFAs explained 91％,94％,and 73％ of the variability in SOC content,respectively.The higher F/B PLFA ratio in JP favored more soil C storage,leading to faster ecosystem recovery compared to either AG or DF.The F/B PLFA ratio could be used as an early indicator of ecosystem recovery in response to disturbance,particularly in relation to land-use change.