Ovarian follicular development in cattle selected for twin ovulations and births

Comparisons of numbers of antral ovarian follicles and corpora lutea (CL), of blood hormone concentrations, and of follicular fluid steroid concentrations and IGFBP activity were conducted between cows selected (twinner) and unselected (control) for twin births to elucidate genetic differences in the regulation of ovarian follicular development. Ovarian follicular development was synchronized among cows by a single i.m. injection of PGF2alpha on d 18 of the estrous cycle; six cows per population were slaughtered at 0, 24, 48, and 72 h after PGF2alpha. Jugular vein blood was collected from each animal at PGF2alpha injection and at 24-h intervals until slaughter. Ovaries of twinner cows contained more small (< or = 5 mm in diameter, P < 0.05), medium (5.1 to 9.9 mm, P < 0.05), and large (> or = 10.0 mm, P < 0.01) follicles and more (P < 0.01) CL than ovaries of controls. Follicular fluid concentrations of estradiol, androstenedione, testosterone, and progesterone reflected the stage of follicular development and were similar for twinner and control follicles at the same stage. Earlier initiation of follicular development and/or selection of twin-dominant follicles in some twinner cows resulted in greater concentrations of estradiol in plasma at 0, 24, and 48 h and of estradiol, androstenedione, and testosterone in follicular fluid of large follicles at 0 h after PGF2alpha for twinner vs. control cows (follicular status x time x population, P < 0.01). Binding activities of IGFBP-5 and -4 were absent or reduced (P < 0.01) in follicular fluid of developing medium and large estro-gen-active (estradiol:progesterone ratio > 1) follicles but increased with atresia. Only preovulatory Graafian follicles lacked IGFBP-2 binding, suggesting a possible role for IGFBP-2 in selection of the dominant follicle. Concentrations of IGF-I were twofold greater (P < 0.01), but GH (P = 0.10) and cholesterol (P < 0.05) were less in blood of twinners. Three generations of selection of cattle for twin ovulations and births enhanced ovarian follicular development as manifested by increased numbers of follicles within a follicular wave and subsequent selection of twin dominant follicles. Because gonadotropin secretion and ovarian steroidogenesis were similar for control and twinner cattle, enhanced follicular development in twinners may result from decreased inhibition by the dominant follicle(s), increased ovarian sensitivity to gonadotropins, and/or increased intragonadal stimulation, possibly by increased IGF-I.     

Possible role of IGF2 receptors in regulating selection of 2 dominant follicles in cattle selected for twin ovulations and births

Abundance of IGF-2 receptor (IGF2R), FSH receptor (FSHR), and LH receptor (LHCGR) mRNA in granulosa cells (GCs) or theca cells (TCs) or both cells as well as estradiol (E₂), progesterone (P₄), and androstenedione concentrations in follicular fluid were compared in cows genetically selected (Twinner) or not selected (control) for multiple ovulations and twin births. Cows were slaughtered at day 3 to 4 (day 3) and day 5 to 6 (day 5) of an estrous cycle, and ovaries, follicular fluid, GCs, and TCs were collected. The two largest (F1 and F2) E₂-active (EA) and E₂-inactive (EI) follicles were selected according to their E₂-to-P₄ ratio and diameter. Androstenedione levels in EA F1 and F2 follicles were 5-fold greater (P < 0.05) in Twinner cows than in control cows on day 3 but did not differ on day 5. Twinner cows also had greater (P < 0.05) E₂ and P₄ concentrations, whereas steroid levels in EI follicles did not differ (P > 0.10) between genotypes. In EA F2 follicles, IGF2R levels in GCs were greater (P < 0.05) in control cows than in Twinner cows on day 3 and day 5, whereas IGF2R mRNA in TCs did not differ (P > 0.10). On day 3, FSHR mRNA levels were greater (P < 0.05) in GCs of EA F1 and EI F2 follicles of control cows than of Twinner cows. LH receptor mRNA expression was less in GCs and greater in TCs of EA F2 follicles in control cows than in Twinner cows (P < 0.05). We hypothesize that reduced GC IGF2R expression in F2 follicles of Twinner cows may play a role in the development of 2 or more dominant follicles.     

Effects of twinning on postpartum reproductive performance in cattle selected for twin births

The effects of twinning, dystocia, retained placenta, and body weight on postpartum reproduction were evaluated for 3,370 single and 1,014 twin births. Females were bred by AI for 40 d followed by 20 or 30 d of natural service with equal numbers bred and calved in spring and fall. Percentage of dams cyclic by the end of the AI period was lower (P<.05) for dams birthing and nursing a single calf (92.4%) than for dams birthing twins and nursing zero (98.7%) or two (94.7%) calves. Whereas the interval from parturition to first estrus was shorter (P<.01) for dams birthing and nursing a single (56.9 d) than for dams birthing twins and nursing one (68.5 d) or two (69.6 d) calves, length of the interval was further reduced by dystocia in nonlactating dams of either twins or singles (type of birth x dystocia, P<.05). Ensuing pregnancy rates were also affected by type of birth and dystocia. Without dystocia, dams birthing and nursing a single calf had a higher pregnancy rate (79.2%) than dams birthing twins and nursing one (61.7%) or two (66.3%) calves, whereas the lower ensuing pregnancy rates associated with dystocia in dams of singles (71.9%) resulted in similar rates among dams of singles and twins with dystocia (type of birth x dystocia; P<.01). Having a retained placenta resulted in a lower incidence of (93.5 vs. 96.4%, with vs. without; P<.05) and a longer interval to (64.7 vs. 59.2 d; P<.01) estrus while reducing subsequent pregnancy rates (X = 9.6%) in 3 of the 7 yr evaluated (retained placenta x year, P<.01). Because all parous females were bred during the same calendrical period, the shorter gestation length for twin calves (275.6 vs. 281.3 d) resulted in a longer interval from parturition to conception for twin births, whereas means for conception date differed by only 2 d between dams of twins and singles. Furthermore, a reduction (P<.01) in the interval to conception occurred with dystocia in dams of singles (89.3 vs. 85.0 d, without vs. with dystocia) and of twins nursed by zero (116.9 vs. 83.5 d), one (100.2 vs. 92.8 d), or two (96.1 vs. 97.2 d) calves. Another detriment to fertility was the higher incidence of fetal mortality or abortions associated with twin vs. single pregnancies (12.4 vs. 3.5%; P<.01). However, despite the lower conception rates for dams of twins, the increased prolificacy provides an opportunity to increase total beef production with a twinning technology.     

Fetal development in cattle with multiple ovulations.

Treatment of lactating and nonlactating parous cows (n = 379) with 12 mg of FSH-P to evaluate development of multiple bovine fetuses resulted in ovulation rates ranging from 1 to 27 corpora lutea (CL). Fertilization rate (i.e., ova fertilized at 6 to 8 d postmating, 80.0%) was not affected by ovulation rate. The percentage of fetuses developing normally at 51 to 53 d postmating decreased (P less than .01) as ovulation rate increased; 1 CL, 100.0%; 2 CL, 100.0%; 3 CL, 66.7%; 4 CL, 45.8%; 5 CL, 33.3%; 6 to 10 CL, 13.6%; and greater than 10 CL, 8.9%. Of the 86 cows permitted to calve, 47 produced singles, 22 twins, 9 triplets, 7 quadruplets, and 1 quintuplets. Calf birth weight and gestational length decreased (P less than .01) as the number of calves born increased from one to two to three. Smaller decreases (P less than .05) in birth weight occurred among triplets, quadruplets, and quintuplets, whereas gestational length did not differ (P greater than .1) among these groups. Systemic progesterone concentrations in the dam were proportional (P less than .01) to the number of fetuses in utero between d 126 and 266 for dams gestating one, two, or three or more fetuses; estrone sulfate was lower (P less than .01) in dams with one than in those with two or more fetuses. Placental weight (i.e., cotyledons plus intercotyledonary membranes) per fetus at 52 +/- 1 d of gestation and at term decreased as the number of fetuses increased. The chorioallantoic membranes were often fused among multiple fetuses and contained either all viable or all dead fetuses, but not both, within the same anastomosed placental unit. These results suggest that ovulation rate is the first limiting factor to increasing cow productivity for beef cattle because some bovine females had the capacity to gestate up to three fetuses per uterine horn, or a total of five fetuses, above which pregnancy was terminated.     

Atresia coli in a twin calf

Atresia coli was diagnosed in a newborn calf. The calf was an identical twin, as evidenced by identical blood type and identical electrophoretic patterns for hemoglobin, transferrin, and amylase. The other calf was clinically normal. The calves resulted from embryo transfer and represented cleavage of a single embryo in the recipient animal. These findings indicate atresia coli in this calf was probably not hereditary.     

Increased abundance of aromatase and follicle stimulating hormone receptor mRNA and decreased insulin-like growth factor-2 receptor mRNA in small ovarian follicles of cattle selected for twin births

Cattle genetically selected for twin ovulations and births (Twinner) exhibit increased ovarian follicular development, increased ovulation rate, and greater blood and follicular fluid IGF-1 concentrations compared with contemporary cattle not selected for twins (Control). Experimental objectives were to 1) assess relationships among aromatase (CYP19A1), IGF-1 (IGF1), IGF-2 receptor (IGF2R), and FSH receptor (FSHR) mRNA expression in small (≤5 mm) antral follicles and 2) determine their association with increased numbers of developing follicles in ovaries of Twinner females. Ovaries were collected from mature, cyclic (d 3 to 6) Twinner (n = 11), and Control (n = 12) cows at slaughter and pieces of cortical tissue were fixed and embedded in paraffin. Expression of mRNA was evaluated by in situ hybridization using (35)S-UTP-labeled antisense and sense probes for CYP19A1, FSHR, IGF1, and IGF2R mRNA. Silver grain density was quantified within the granulosa and theca cells of individual follicles (2 to 7 follicles/cow) by Bioquant image analysis. Follicles of Twinners tended to be smaller in diameter than Controls (1.9 ± 0.1 vs. 2.3 ± 0.1 mm; P = 0.08), but thickness of granulosa layer did not differ (P > 0.1) by genotype. Relative abundance of CYP19A1 (P < 0.01) and FSHR (P < 0.05) mRNA was greater in granulosa cells of Twinners vs. Controls, respectively, whereas IGF2R mRNA expression was less in both granulosa (P < 0.01) and theca (P < 0.05) cells in follicles of Twinners vs. Controls, respectively. Abundance of CYP19A1 mRNA in granulosa cells was correlated negatively with IGF2R mRNA expression in both granulosa (r = -0.33; P < 0.01) and theca (r = -0.21; P = 0.05) cells. Expression of IGF1 mRNA was primarily in granulosa cells, including cumulus cells, and its expression did not differ between Twinners vs. Controls (P > 0.10). Detected increases in CYP19A1 and FSHR, but not IGF1, mRNA expression along with decreases in IGF2R mRNA expression in individual follicles of Twinners support the hypothesis that increased follicular development and steroidogenesis in Twinner females result from increased extra-ovarian IGF-1 production. Furthermore, a reduction in follicular IGF2R mRNA expression accompanied by a reduction in receptor numbers would increase availability of free IGF-2 and its stimulation of follicular development in Twinners.     

The effects of genotype and selected environmental factors on colostrum production and intake in cattle

The aim of this study was to determine effects of genotype and selected environmental factors on colostrum production, intake, and efficiency in the cattle. The investigations were conducted on 67 dam-calf pairs. All cows were of Polish Holstein-Friesian breed, the Black-and-White variety (PHF-HO), whereas calves were sired by bulls of the following breeds: PHF-HO, Polish Holstein-Friesian of the Red-and-White variety (PHF-RW), Jersey (JE), Montbéliarde (MB) and Limousine (LI). The colostrum was collected from cows and offered to calves "from bucket" thrice a day. The amount of produced colostrum considerably exceeded the ability of its consumption by calves. Low share of HF genes in the cow, older cow's age and calving in the period from January to April appeared to be favorable factors for colostrum production. Calves born to cows with low HF gene share drank more colostrum than calves originating from cows with higher gene share of this breed. Crosses with JE drank the highest amount of the colostrum in relation to body weight, while MB-sired calves drank the highest amount in absolute terms. Colostrum intake was positively correlated with the dam's age. Furthermore, it was shown that in case of the first colostrum intake the most favorable period was from May to September, while in cases of total intake in the first day of life and mean intake in the first 5 days of life it was the period from January to April. Crosses with JE were characterized by extremely high use of colostrum per unit of body weight gain.     

Variation among twin beef cattle in maintenance energy requirements

The genetic variation in energy expenditures of cattle at fasting (FHP) and maintenance (MEm) was determined by using 12 pairs of monozygous twins at 20 mo of age. The pairs were of two breed types, eight Angus x Hereford (three steers, five heifers) and four Barzona x Hereford (three steers, one heifer). The heifers were 132 +/- 13 d pregnant at the time of measurement. The pairs were fed at 1.15 x maintenance energy requirements for a minimum of 30 d prior to heat production (HP) measurements in dual indirect respiration calorimetry chambers. The diet fed was cracked corn:alfalfa hay (45:55) with a determined ME of 2.47 +/- .02 Mcal/kg DM. This diet was fed individually for 7 d prior to and during two consecutive 22-h HP measurements. The animals then were fasted for 2 d and fasting heat production measurements (FHP) were made on d 3 and 4 of the fast. Metabolizable energy required for MEm was calculated iteratively by assuming a semi-log relationship between HP and metabolizable energy intake. There were no differences (P greater than .10) in measured energy expenditures due to different breed type. The FHP and efficiency of ME use for MEm (Km) were similar between sexes, although heifers had lower (P less than .025) MEm than steers. Twin pair effects were detected for FHP (P less than .005) and MEm (P less than .05) but not for Km. Broad sense heritability estimates were calculated as the intraclass correlation between members of monozygous twin pairs. Heritability estimates for MEm, FHP, and Km were .52 +/- .22, .75 +/- .13, and .34 +/- .27, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)     

Productivity and profitability of twin births in beef cattle

Data from 1,277 single and 85 twin calvings, occurring in both spring and fall from 1980 through 1987, were used to examine the productivity and profitability associated with twin births in beef cattle. Pregnancies in pure and crossbred cattle resulted from both AI and embryo transfer. Cows and calves were confinement-housed. Cows were individually fed to specification. Calves were given ad libitum access to creep feed and those born in 1986 and 1987 were fed to slaughter. The influence of birth number of gestation length, total calf birth and weaning weights, lactation yield, and cow fed intake during both the dry and lactating periods were examined. Twin-bearing cows had their gestation length shortened by 6.4 d (2%); yielded 25.5 (59) and 186.0 kg (73%) more weight of calf at birth and weaning, respectively; had lactation yield and lactation feed intake increased by 25 and 20%, respectively; and had precalving (dry) period feed intake no different from their single-bearing counterparts (P = .12). Postweaning growth was not different for single and twin calves (P = .50); twin gain, relative to initial size, was higher. Feedlot feed intake of twins was 85% of that for singletons (P = .20). Twins were 90% of singleton live weight at slaughter and yielded 93% of singleton hot carcass weight (P = .12). Twins were slightly older and significantly leaner at slaughter. Returns less feed costs showed twin births to be associated with increased profit for cow-calf programs. Returns less feed and overhead costs were higher for twin calves than for singles in the feedlot.     

Beta-ligands in cattle production

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Feeding the growing population in a sustainable, environmentally-friendly manner will require implementation of management strategies and metabolic effectors that facilitate efficient protein deposition in livestock (Dunshea et al., 2016). Beta adrenergic receptor ligands fed to cattle improve live animal performance that translates into positive effects on muscle protein accretion and carcass parameters (Bittner et al., 2016; Bittner et al., 2017; DeClerck et al., 2020; Lockard et al., 2020). A recent review discusses the differences in the β-ligands, the receptors they interact with and the resulting tissue outcomes (Dilger et al., 2021).     

Follicle stimulating hormone, luteinising hormone and progesterone concentrations in the blood of thoroughbred mares exhibiting single and twin ovulations

The concentrations of follicle stimulating hormone, luteinising hormone and progesterone were measured in serial blood samples taken throughout one or more oestrous cycles from 12 Thoroughbred mares, some of which exhibited single and others twin ovulations. The resulting profiles clearly demonstrated that no simple relationship exists between circulating gonadotrophin levels and subsequent ovulation rate in the mare. However, plasma progesterone concentrations during dioestrus are, as expected, higher following twin than single ovulations. The findings suggest that the underlying cause of twin ovulation in some mares may exist at the ovarian end of the pituitary-ovarian axis which controls follicular development and ovulation.     

Effect of various degrees of dystocia in dairy cattle on calf viability, milk production, fertility and culling

The objective of this study was to analyse the effect of dystocia on calf viability, milk production, fertility and culling under field (farm) conditions in a retrospective case-control study. A total of 253 calvings that were attended by a veterinarian were classified into three categories: mild dystocia (MD), severe dystocia (SD) and caesarean section (CS). For every case of dystocia, a control was chosen from the same farm and matched for calving month, breed and parity. Cases and controls were compared concerning calf viability, milk production, fertility and culling. The proportion of stillbirths was significantly higher in cows with dystocia compared with their controls. However, the difference was lower in CS cows than in the other two groups. MD had no significant effects on milk production, fertility and culling. There were no obvious effects of SD on monthly test day milk production, while CS cows produced significantly less milk than their controls. Compared with controls, less SD (50.0% versus 61.6%) and CS cows (48.6% versus 70.3%) conceived until 200 days in milk and more CS cows (31.1% versus 16.2%) were culled in the same time interval. These data demonstrate the scope of economic and animal welfare opportunities associated with the reduction of dystocia by management and breeding.