小麦籽粒Zn、Fe、Mn、Cu含量的基因型和环境差异及与产量关系的研究

小麦籽粒中微量营养元素含量的高低直接关系到植株的生长发育和人们的饮食健康。本研究以来自河南省5个地区的17个小麦品种(系)为材料,采用酸消解-原子吸收分光光度法测定了籽粒中Zn、Fe、Mn、Cu含量,分析了籽粒微量元素含量的基因型和环境差异及其与产量性状的相关关系。结果表明,小麦籽粒Zn、Fe、Mn、Cu含量分别为38.39±12.57μg·g-1、79.13±49.45μg·g-1、35.24±11.72μg·g-1和4.84±0.78μg·g-1,籽粒Fe含量的变异系数最大,Cu含量的变异系数最小。方差分析表明,基因型、环境以及基因型与环境的互作对籽粒微量元素含量的影响均达极显著水平。对于籽粒Zn、Mn、Cu含量,环境因素的影响起主要作用;对于籽粒Fe含量,基因型与环境互作的影响是主要的。依照Eberhart-Russell模型分析了籽粒微量元素含量的品种稳定性,结果表明籽粒微量元素含量相对稳定的品种,其含量通常较低;在参试17个品种中,"濮99084"籽粒微量元素含量的环境稳定性较高。相关分析表明,籽粒微量元素含量与蛋白质含量呈遗传正相关,与千粒重呈遗传负相关,与产量呈表型正相关,表明同步提高籽粒微量元素含量、蛋白质含量及籽粒产量是可行的。     

叶面喷施硒肥对小麦籽粒及面粉硒含量的影响

为了探明叶面喷施硒肥对小麦籽粒及制粉各粉路硒含量的影响,本研究选用4个小麦品种,在拔节和抽穗期分2次喷施0.017%亚硒酸钠叶面肥,并对成熟期籽粒进行制粉,分析各粉路的总硒、无机硒和有机硒含量。结果表明,叶面喷硒对小麦籽粒和面粉的总硒、有机硒和无机硒含量的影响存在品种间差异。喷施硒肥后,4个小麦品种籽粒的总硒含量提高了26.4~35.8倍,但小麦籽粒产量无显著变化。皮磨粉、心磨粉、统粉、小麸和大麸5种粉路中,小麸的总硒含量最高,其次为大麸,皮磨粉、心磨粉和统粉的总硒含量较低。与硬质小麦相比,软质小麦出粉率低,面粉的总硒量损失较多。经制粉,对照和喷硒样本的总硒积累量分别损失52.97%~69.28%和36.73%~44.51%。对照样本中,各粉路的有机硒含量略高于无机硒含量;而喷硒处理样品中,除宁麦20的小麸外,各粉路的有机硒含量均略低于无机硒含量。本研究揭示了制粉粉路硒分布规律,这对于富硒小麦的加工利用具有重要的指导作用。     

不同制粉方式对运黑161全麦粉加工特性的影响

为探讨不同制粉方式及不同粒度对黑小麦流变学特性及加工品质的影响,将运黑161黑小麦经直接粉碎法和回添法制得的全麦粉分别过80、100、120目筛,以运黑161黑小麦面粉为对照,分析全麦粉淀粉含量、水合特性、发酵特性以及流变学特性。结果表明,直接粉碎且过80目筛的全麦粉支链淀粉含量最高,为80.18%,破损淀粉含量最低,为9.93%,溶解度、膨润力最小,分别为2.5%和3%。回添法制备的全麦粉面团发酵高度、持气率小于直接粉碎法。直接粉碎且过80目筛全麦粉的粉质质量指数最高,为59.5,形成时间和稳定时间分别为3.45和3.2 min,较接近于黑小麦面粉,且该全麦粉的阻力值、最大阻力值在醒发时间135 min时最大,用该全麦粉制作的面包感官得分最高。综上,以直接粉碎且过80目筛制备的全麦粉为原料制作的全麦面包品质较好。本研究结果为黑小麦全麦面包研发提供了理论基础。     

生态环境与施氮量协同对小麦籽粒微量元素含量的影响

【目的】铁、 锰、 铜、 锌等微量元素含量的高低不仅直接关系到小麦植株的生长发育也直接影响到小麦籽粒的营养价值。不同地区、 品种以及种植方式小麦微量元素营养品质不稳定。本试验主要研究不同生态环境与栽培措施协同对小麦籽粒微量元素含量的影响,以期为小麦高产优质生产提供参考。【方法】试验位于生态环境差异较大的北京、 河北任丘和河北赵县,采用裂区设计,以试验点为主区,即任丘、 北京、 赵县; 施氮量为副区,设5个氮素水平0、 90、 180、 270、 360 kg/hm2; 小麦品种为副副区,为济麦20、 皖麦38、 京冬8号、 中麦8号。收获后于每个小区中随机取样测定籽粒样品中Fe、 Mn、 Cu、 Zn等4种微量元素的含量。比较微量元素含量的差异显著性,分析不同试点生态条件与施氮量对小麦微量元素含量的影响,探讨不同生态环境下小麦微量元素含量的稳定性以及氮肥的调控补偿效应。【结果】 1）小麦籽粒中铁、 锰、 铜、 锌微量元素含量受生态环境、 栽培因素及品种遗传因素的共同作用,各试验因子均对其有显著影响。其中以生态环境的影响最大,生态环境差异越大,对小麦微量元素的影响作用越大。降水量可以影响小麦对铁和锰的吸收, 锌则受土壤有机质含量和试点纬度影响较大; 土壤中的钾可以促进小麦对铁、 锰、 铜、 锌的吸收利用。2）在0~360 kg/hm2 施氮范围内,氮肥有利于促进小麦对土壤中铁、 铜和锌的吸收,不利于锰的吸收积累。适当施用氮肥有利于缩小小麦籽粒铁、 锰、 铜元素含量的生态环境变异,提高其生态稳定性,但对锌元素试点间稳定性影响较小。施用氮肥对不同小麦品种微量元素含量差异有不同程度的调节效应,有利于缩小小麦品种间铁、 锰、 锌含量的差异。【结论】栽培环境对小麦籽粒微量元素含量的影响大于遗传因素,即小麦籽粒微量元素含量有较强的栽培可塑性。施氮有利于弥补生态环境或品种差异引起的微量元素含量不稳定性,调节品种差异并补偿生态环境对小麦造成的不利影响。适宜的栽培环境对提高小麦籽粒微量元素含量有明显效果。     

石灰性土壤上不同小麦基因型对施锌的反应

为比较石灰性土壤上面包型小麦(Triticum aestivum L.)对施锌的反应,选择20种小麦基因型在温室中进行了土培试验。结果表明,在杨凌当地土壤有效锌含量水平(0.6 mg kg-1左右)下,施Zn对供试基因型小麦植株生长量均无明显影响,由于小麦根冠比主要受基因型控制,施锌对它的影响也很小。然而,供Zn显著提高了所有基因型小麦植株各部分的Zn含量和吸Zn量,根、茎、叶、籽粒中Zn含量增加幅度分别达0.22～3.22倍、0.26～2.82倍、0.10～3.84倍、0.10～0.84倍,整株吸Zn量均大幅度增加,幅度在28.8%～219.3%之间,平均增加104.8%。施Zn后不同基因型的Zn转运率有明显差异,范围在13.5%～90.2%之间,收获籽粒的6种基因型小麦对锌的转运率明显高于其它正处于抽穗期的基因型,表明灌浆期是Zn从根部向穗部转运的关键时期。施锌也显著提高了土壤有效锌含量。总之,对生长在有效锌含量不高的石灰性土壤上的小麦施锌是改善其营养品质的重要措施。     

施纳米硒对小麦籽粒硒含量及其品质性状的影响

【目的】研究基施纳米硒肥对不同小麦品种籽粒百粒重、硒含量、硒形态,其他矿质元素以及面粉糊化特性的影响,为利用纳米硒肥进行小麦硒生物强化提供参考。 【方法】选择 110 份小麦品种 (系),在扬州大学农学院网室种植,采用的施肥方式为基施纳米硒肥,设每千克土施 Se 0 (CK)、100 (Se100)、150 (Se150) mg 2 次重复,随机区组设计。小麦成熟期收获籽粒,测定百粒重。利用离子发射光谱 – 原子吸收仪测定了籽粒 Se、Ca、Mg、Cu、Fe、Mn、Zn 及 S 含量。选择高硒处理中硒含量前 10 名的品种,利用 LC-UV-AFS 测定了籽粒中不同形态硒的含量。随机挑选 59 个品种并利用 RVA 仪 (快速粘度分析仪) 测定其面粉糊化特性的特征值。利用 MATLAB 和 SPSS 软件进行数据分析。 【结果】基施硒 0、100、150 mg/kg 土,小麦籽粒的百粒重均值分别为 3.78、4.11 和 3.70 g,籽粒中总硒含量分别为 2.00、12.46 和 17.35 mg/kg。籽粒中的硒主要以有机态形式存在,以硒蛋氨酸含量最多,其次是硒甲基化半胱氨酸和少量的硒半胱氨酸,无机态硒仅微量存在于极少数品种中。基施硒肥对测定的矿质元素的吸收积累既有协同也有拮抗作用,对小麦面粉的糊化特性没有显著影响。糊化特性取决于基因型,不同品种间存在极显著差异。 【结论】硒肥可以提高小麦籽粒中的硒含量,但增幅因品种而异;硒强化后籽粒中硒主要以有机态形式存在;低浓度硒处理可以提高小麦籽粒的百粒重,且不会影响小麦的面粉糊化特性。     

不同生态区对油菜薹营养品质的影响及摘薹后产量表现

为明确油菜薹的营养品质、不同“油蔬”两用型油菜薹品质、菜薹产量与环境之间的关系,通过测定碳水化合物、维生素类物质和微量元素含量,比较了油菜薹、红菜薹和白菜薹3种菜薹的营养品质;6个“油蔬”两用型油菜品种(系)油菜薹营养品质;杭州余杭和丽水龙泉2个生态区的油菜薹产量、籽粒产量以及经济效应。结果表明,油菜薹可溶性糖、3种维生素类物质(维生素C、B₁和B₆)、有效Zn和全Se含量分别是白菜薹的1.82、2.61、4.44、1.68、1.81和1.67倍,表明油菜薹具有优良的营养品质。6个油菜品种(系)的油菜薹的糖类物质(除果糖)和维生素类物质含量在丽水龙泉均显著高于杭州余杭。就微量元素而言,有效Fe、有效Cu、有效Zn和全Se含量在杭州余杭显著高于丽水龙泉,有效Mn含量则相反。说明油菜薹中需要经过代谢、运转的营养物质(如碳水化合物)在丽水龙泉具有显著优势,而从土壤中吸收的营养元素则相反。“油蔬”两用型油菜品种(系)籽粒和菜薹产量受生态区影响显著。通过增加采收一季菜薹,油菜的经济效益可增加一倍。本研究为“油蔬”两用型油菜品种利用以及不同生态区优质高产栽培提供了理论依据。     

野生二粒小麦及其后代富含微量营养元素种质资源的筛选鉴定

由于主要粮食作物中微量营养元素(特别是Zn 和Fe)的缺乏导致全世界范围内的人类微量营养元素缺乏症。野生二粒小麦(Triticum turgidum ssp. dicoccoides)是提高小麦籽粒Zn、Fe、蛋白质含量及改良小麦其他性状的重要种质资源。本研究以4 份野生二粒小麦及32 份野生二粒小麦与普通小麦(Triticum aestivum L.)杂交后代为材料, 进行籽粒中富含Zn、Fe 和蛋白质种质资源的筛选和鉴定。野生二粒小麦的后代材料表现出广泛的遗传变异。籽粒Zn、Fe 含量和蛋白质含量之间均呈极显著正相关关系, 说明这些性状能够通过育种方式达到共同提高的目的。试验共筛选到17 份Zn、Fe 和蛋白质含量均显著高于对照品种"石4185"的材料, 说明将来自野生二粒小麦的外源基因导入到普通小麦是提高小麦籽粒Zn、Fe 和蛋白质含量的有效途径。其中5份野生二粒小麦与普通小麦的杂交后代表现出较高籽粒Zn、Fe 和蛋白质含量的同时, 也具有较高的产量和优良的农艺性状。这些材料的获得为小麦生物强化育种提供了重要的物质基础。     

黄河三角洲贝壳堤岛贝壳沙中微量元素含量及形态特征

通过对不同植被覆盖、不同剖面层次贝壳沙样品的采集与分析,研究了黄河三角洲贝壳堤岛贝壳沙中微量元素Fe,Mn,Cu,Zn的含量及形态特征.结果表明:贝壳沙中全Fe含量明显高于全国土壤中Fe的含量均值,而Mn,Cu,Zn 3种元素却远低于全国土壤中的含量.无论是表层样品还是剖面样品.微量元素含量均具有较大变异,且表层以下剖面样品4种元素含量高于表层样品含量.受植物生长的影响,有机枯落物较多的表层贝壳沙样品,其元素含量相应高于没有植被覆盖的表层样品.不同剖面样点自上而下,微量元素含量变化规律并不一致,其全量与有效态含量的相关性大小也不一样.Mn,Cu两种元素全量和有效态含量之间达到极显著相关水平(P＜0.01),而Fe,Zn两种元素全量和有效态含量之间的相关性却不显著,这可能是受元素种类与环境条件的双重影响所致.     

放牧对冷蒿根际土壤营养元素的影响

为了揭示放牧扰动对内蒙古典型草原冷蒿(Artemisia frigida Willd.)根际土壤的影响,采用小区控制放牧强度(对照CK、轻度放牧LG和重度放牧HG)处理,测定了不同放牧强度冷蒿根际土壤和非冷蒿根际土壤养分状况和微量元素含量的变化。结果表明:冷蒿根际土壤pH值显著小于非冷蒿根际土壤,随放牧强度的增加冷蒿根际土壤pH值呈降低趋势,有机质含量显著高于非冷蒿根际土壤;冷蒿根际土壤有效N、P、K和全N含量极显著高于非冷蒿根际土壤,轻度放牧冷蒿根际土壤与对照相比分别增加了41.7%,20.6%,5.3%和47.0%(P0.05);冷蒿根际土壤微量元素Fe、Mn和Zn含量与非冷蒿根际土壤无显著差异,有效微量元素含量极显著高于非冷蒿根际土壤,随放牧强度的增加冷蒿根际土壤有效微量元素Fe、Mn、Cu、Zn含量逐渐升高,重度放牧与对照相比分别增加了32.4%,39.5%,53.0%和37.3%(P0.05);土壤有效N、P、K及微量元素Fe、Mn、Cu、Zn含量与土壤pH值呈极显著负相关(P0.01),与土壤有机质含量呈极显著正相关(P0.01)。重度放牧后冷蒿可通过改变土壤pH值,促进有机质的分解,提高土壤有效养分及有效微量元素的含量,改善冷蒿根际土壤中养分状况,这可能是冷蒿形成单一优势种群的原因之一。     

Spelt (Triticum aestivum ssp. spelta) as a source of breadmaking flours and bran naturally enriched in oleic acid and minerals but not phytic acid

The nutritional value of breadmaking cereal spelt (Triticum aestivum ssp. spelta) is said to be higher than that of common wheat (Triticum aestivum ssp. vulgare), but this traditional view is not substantiated by scientific evidence. In an attempt to clarify this issue, wholemeal and milling fractions (sieved flour, fine bran, and coarse bran) from nine dehulled spelt and five soft winter wheat samples were compared with regard to their lipid, fatty acid, and mineral contents. In addition, tocopherol (a biochemical marker of germ) was measured in all wholemeals, whereas phytic acid and phosphorus levels were determined in fine bran and coarse bran samples after 1 month of storage. Results showed that, on average, spelt wholemeals and milling fractions were higher in lipids and unsaturated fatty acids as compared to wheat, whereas tocopherol content was lower in spelt, suggesting that the higher lipid content of spelt may not be related to a higher germ proportion. Although milling fractionation produced similar proportions of flour and brans in spelt and wheat, it was found that ash, copper, iron, zinc, magnesium, and phosphorus contents were higher in spelt samples, especially in aleurone-rich fine bran and in coarse bran. Even though phosphorus content was higher in spelt than in wheat brans, phytic acid content showed the opposite trend and was 40% lower in spelt versus wheat fine bran, which may suggest that spelt has either a higher endogenous phytase activity or a lower phytic acid content than wheat. The results of this study give important indications on the real nutritional value of spelt compared to wheat. Moreover, they show that the Ca/Fe ratio, combined with that of oleate/palmitate, provides a highly discriminating tool to authenticate spelt from wheat flours and to face the growing issue of spelt flour adulteration. Finally, they suggest that aleurone differences, the nature of which still needs to be investigated, may account for the differential nutrient composition of spelt and wheat.     

Composition and Distribution of Pentosans in Millstreams of Different Hard Spring Wheats

Six commercially grown samples of hard spring wheat were milled using a tandem Buhler laboratory mill. Individual flour streams and branny by‐products, as well as whole‐grain wheat and straight‐grade flour, were characterized in terms of total (TP), water‐extractable (WEP), and water‐unextractable (WUP) pentosans. One representative cultivar sample was analyzed for its ratio of arabinose to xylose (A/X). TP and WEP of whole grain wheat of the six samples had ranges of 5.45–7.32% and 0.62–0.90% (dm), respectively. Neither TP nor WEP of whole grain was related to ash content variation. There was significant variation in the distribution and composition of pentosans in 16 millstreams of all the wheat samples, including bran and shorts fractions; TP and WEP contents had ranges of 1.69–32.4% and 0.42–1.76% (dm), respectively. When ash contents exceeded ≈0.6% (dm), strong positive correlations were obtained between ash and TP contents, and between ash and WUP contents for all the millstreams. Among bran and shorts fractions, TP and WUP content increased in the order of coarse bran > fine bran > shorts; while WEP, WEP/WUP and A/X showed the opposite pattern of variation of shorts > fine bran > coarse bran. Bran and shorts fractions had pentosan contents several times higher than would be predicted from the relationship between pentosan and ash contents of the flour streams. Pentosans therefore represented a much more sensitive marker of flour refinement compared with ash content. Pentosans of endosperm were substantially different in their extractability and composition from those of bran. On this basis, different functionalities of pentosans of bran and endosperm would be expected. Results demonstrated the importance of milling extraction and millstream blending in the functionality and quality of wheat flour for breadmaking.     

Plant Sterols in Cereals and Cereal Products

The total plant sterol contents (free sterols and covalently bound structures) of the main cereals cultivated in Finland were determined. Furthermore, sterol contents were determined for different flour and bran fractions in the milling process of wheat and rye, as well as plant sterol contents in various milling and retail bakery products. The sample preparation procedure included acid and alkaline hydrolysis to liberate sterols from their glycosides and esters, respectively. Free sterols were extracted and, after recovery using solid‐phase extraction, derivatized to trimethylsilyl ethers for gas chromatography (GC) analysis. We used GC with a mass spectrometer (MS) for identification. When two cultivars of rye, wheat, barley, and oats grown in the same year were compared, the highest plant sterol content was observed in rye (mean content 95.5 mg/100 g, wb), whereas the total sterol contents (mg/100 g, wb) of wheat, barley, and oats were 69.0, 76.1, and 44.7, respectively. In addition, the 10 rye cultivars and breeding lines compared had total sterol contents of 70.7–85.6 mg/100 g. In the milling process of rye and wheat, the plant sterols fractionated according to the ash content of the corresponding milling product. In all cereal grain and milling product samples, sitosterol was the main sterol. The level of stanols differed in the different milling process samples; it was lower in the most refined rye and wheat flours (≈15%) than in the bran fractions (≈30% in the bran with 4% ash content). Rye bread with whole meal rye flour as the main or only ingredient was a good source of sterols. Sterol content was higher than that of wheat bread, whereas plant sterol content of other bakery products was affected by the type and amount of fat used in baking.     

氮肥用量和喷施锌钾肥对小麦籽粒锌含量及生物有效性的影响

[目的]小麦籽粒锌(Zn)含量普遍较低,在没有外源锌施用措施下难以满足以小麦为主食人群健康所需.探索提升小麦籽粒Zn含量,尤其是其加工产品面粉Zn含量和Zn生物有效性的农艺措施,具有实际重要的意义.[方法]于2013、2014年分别在陕西杨凌示范区和三原试验站进行小麦田间试验,两地均为潜在缺锌石灰性土壤,DTPA-Zn...     

Nutritional Composition of Iron,Zinc, Calcium,and Phosphorus in Wheat Grain Milling Fractions as Affected by Fertilizer Nitrogen Supply

Fe and Zn deficiencies are global nutritional problems. N supply could increase Fe and Zn concentrations in wheat grain. This study was conducted to determine the impacts of different N rates (0, 122, 174, and 300 kg/ha) on the distribution and speciation of Fe and Zn in wheat grain milling fractions under field conditions. Zn and protein concentrations were increased, whereas Fe was less affected in the flour fractions with increasing N rates. Further analysis with size‐exclusion chromatography coupled with inductively coupled plasma mass spectrometry revealed that Fe and Zn bound to low‐molecular‐weight (LMW) compounds in the flour fractions (probably Fe‐nicotianamine [NA], Fe‐deoxymugineic acid, or Zn‐NA) were less affected by increasing N supply, representing 3.5–10.9% of total Fe and 2.5–56.6% of total Zn. In the shorts fraction, LMW‐Fe was absent, and LMW‐Zn with higher N supply was over twice as high as that in control and 3–27 times as high as that in the other milling fractions. In the flour fractions, the molar ratios of phytic acid (PA)/Fe and PA/Zn (both less than 30.5) decreased, whereas soluble LMW‐Fe/Zn was not affected with increasing N rates.     

Phenolic Content and Antioxidant Activity of Pearled Wheat and Roller‐Milled Fractions

Wheat contains phenolic compounds concentrated mainly in bran tissues. This study examined the distribution of phenolics and antioxidant activities in wheat fractions derived from pearling and roller milling. Debranning (pearling) of wheat before milling is becoming increasingly accepted by the milling industry as a means of improving wheat rollermilling performance, making it of interest to determine the concentration of ferulic acid at various degrees of pearling. Eight cultivar samples were used, including five genotypes representing four commercial Canadian wheat classes with different intrinsic qualities. Wheat was pearled incrementally to obtain five fractions, each representing an amount of product equivalent to 5% of initial sample weight. Wheat was also roller milled without debranning. Total phenolic content of fractions was determined using the modified Folin‐Ciocalteau method for all pearling fractions, and for bran, shorts, bran flour, and first middlings flour from roller milling. Antioxidant activity was determined on phenolic extracts by a method involving the use of the free radical 2,2‐diphenyl‐l‐picrylhydrazyl (DPPH). Total phenolics were concentrated in fractions from the first and second pearlings (>4,000 mg/kg). Wheat fractions from the third and fourth pearlings still contained high phenolic content (>3,000 mg/kg). A similar trend was observed in antioxidant activity of the milled fractions with ≈4,000 mg/kg in bran and shorts, ≈3,000 mg/kg in bran flour, and <1,000 mg/kg in first middlings flour. Total phenolic content and antioxidant activity were highly correlated (R² = 0.94). There were no significant differences between red and white wheat samples. A strong influence of environment (growing location) was indicated. Pearling represents an effective technique to obtain wheat bran fractions enriched in phenolics and antioxidants, thereby maximizing health benefits associated with wheat‐based products.     

麸皮面粉面团的粉质和拉伸特性

为了探讨麸皮对小麦面粉品质的影响,将微粉碎后的燕麦麸细粉、小麦麸细粉与小麦粉按混料配方均匀设计制备麸皮面粉,用粉质仪和拉伸仪测定了麸皮面粉的面团流变学特性,并对麸皮面粉各组分含量与流变学参数间的相关性进行分析。结果表明,随着麸皮含量的增加,麸皮面粉面团的吸水率增大,形成时间变化不大,稳定时间不断减少;弱化度和粉质指数在不同样品变化较大,以样品3粉质特性(即小麦粉88.5%,燕麦麸细粉10.20%,小麦麸1.39%)最优;相同醒发时间下,随着麸皮总含量的增加,面团的拉伸曲线面积、延伸度、拉伸阻力及最大拉伸阻力均呈下降趋势;拉伸参数均随醒发时间而呈上升趋势。与面团粉质特性相关性最显著的是小麦粉含量,其次是小麦麸和燕麦麸细粉含量;小麦粉含量与拉伸特性呈显著正相关,与燕麦麸含量呈显著负相关,与小麦麸含量的相关性不显著。这些研究表明麸皮添加比例能够显著影响面团的流变学特性。     

Effect of Single‐Pass and Multipass Milling Systems on Whole Wheat Durum Flour and Whole Wheat Pasta Quality

Single‐pass and multipass milling systems were evaluated for the quality of whole wheat durum flour (WWF) and the subsequent whole wheat (WW) spaghetti they produced. The multipass system used a roller mill with two purifiers to produce semolina and bran/germ and shorts (bran fraction). The single‐pass system used an ultracentrifugal mill with two configurations (fine grind, 15,000 rpm with 250 μm mill screen aperture; and coarse grind, 12,000 rpm with 1,000 μm mill screen aperture) to direct grind durum wheat grain into WWF or to regrind the bran fraction, which was blended with semolina to produce a reconstituted WWF. Particle size, starch damage, and pasting properties were similar for direct finely ground WWF and multipass reconstituted durum flour/fine bran blend and for direct coarsely ground WWF and multipass reconstituted semolina/coarse bran blend. The semolina/fine bran blend had low starch damage and had desirable pasting properties for pasta cooking. WW spaghetti was better when made with WWF produced using the multipass than single‐pass milling system. Mechanical strength was greatest with spaghetti made from the semolina/fine bran or durum flour/fine bran blends. The semolina/fine bran and semolina/coarse bran blends made spaghetti with high cooked firmness and low cooking loss.     

Phytochemical profile of main antioxidants in different fractions of purple and blue wheat, and black barley

Two pigmented wheat genotypes (blue and purple) and two black barley genotypes were fractionated in bran and flour fractions, examined, and compared for their free radical scavenging properties against 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical cation (Trolox equivalent antioxidant capacity, TEAC), ferric reducing antioxidant power (FRAP), total phenolic content (TPC), phenolic acid composition, carotenoid composition, and total anthocyanin content. The results showed that fractionation has a significant influence on the antioxidant properties, TPC, anthocyanin and carotenoid contents, and phenolic acid composition. Bran fractions had the greatest antioxidant activities (1.9-2.3 mmol TEAC/100 g) in all four grain genotypes and were 3-5-fold higher than the respective flour fractions (0.4-0.7 mmol TEAC/100 g). Ferulic acid was the predominant phenolic acid in wheat genotypes (bran fractions) while p-coumaric acid was the predominant phenolic acid in the bran fractions of barley genotypes. High-performance liquid chromatography analysis detected the presence of lutein and zeaxanthin in all fractions with different distribution patterns within the genotypes. The highest contents of anthocyanins were found in the middlings of black barley genotypes or in the shorts of blue and purple wheat. These data suggest the possibility to improve the antioxidant release from cereal-based food through selection of postharvest treatments.     

Physical and Milling Characteristics of Wheat Kernels After Enzyme and Acid Treatments

The current wheat milling process separates bran from endosperm by passing tempered wheat kernels through successive break rolls and sifters. Using hydrolytic enzymes during tempering degrades bran and aleurone layers and can improve milling efficiency and yield. This study was conducted to evaluate the effects of chemical and enzymatic treatments of wheat kernels before milling on physical and milling characteristics of the resulting wheat and flour quality. Hard wheat kernels were soaked in dilute acid or water and dried back to original moisture before being tempered with enzymes in water. Kernel physical and milling characteristics (600 g) were evaluated. Dilute acid soaking did not affect the 1,000‐kernel weight and diameter but softened treated kernels. When treated kernels were pearled, bran removal was mostly from ends; and the reducing sugar content in enzyme‐treated bran was significantly higher than the control. Compared with the control, acid‐soaked enzyme‐tempered kernels showed small but significant improvement in straight flour yield, with virtually no difference in protein content, and flour color. Chemical and enzyme treatment resulted in higher ash in flour. These differences were not seen in milling of larger batches (1,500 g) of kernels.