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PMI/甘露糖筛选体系在植物转基因中的应用 总被引:4,自引:0,他引:4
介绍了一种可在植物转基因中应用的新型筛选方法———磷酸甘露糖异构酶(PMI)法,与传统筛选体系不同,它以甘露糖为筛选剂对转化细胞进行正筛选。PMI能将甘露糖- 6 -磷酸转化成果糖- 6 -磷酸,使转化细胞以甘露糖为唯一或主要碳源而正常生长;非转化细胞由于不能利用甘露糖而停止生长。该筛选体系受基因型、培养基中其他糖和磷酸根离子浓度及培养条件等因素的影响。目前已应用于多种模式植物和经济作物的转基因筛选,在木本植物甜橙上也首获成功。其检测方法多样,除了常规转基因检测方法外,还可对酶活性进行检测,其中氯酚红法简单可靠,且无需昂贵试剂。安全评估结果表明PMI基因对人体健康和环境无害。PMI/甘露糖筛选体系有望成为植物转基因的又一有效筛选手段。 相似文献
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遵循植物的耐荫习性是营建一个生态稳定的植物群落的前提.目前国内对植物耐荫生理的研究相对落后,并且存在一定的误区.本文从植物的形态特征、解剖结构、叶绿素分布和光化学反应等方面对植物耐荫生理的国内外研究现状进行了综述. 相似文献
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SO2和NO2为我国大气污染主要污染物,城市森林对SO2和NO2具有一定的抵抗能力和吸收净化能力,利用城市森林对大气污染进行修复具有良好的应用前景.文章概述了我国SO2和NO2气体污染的状况,总结了污染物进入植物体的过程及其损伤机理,并从植物抗性和吸附净化能力两方面总结了城市景观树种与SO2和NO2气体污染的关系研究,并基于研究结论提出今后研究可能发展方向,如SO2、NO2与悬浮颗粒物等复合污染对城市树种生长的影响、树种抗性和吸附能力综合研究等. 相似文献
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由CO2等温室气体排放带来的全球变暖问题是目前最严峻的环境问题之一。因此,利用多孔炭材料作为其高效吸附材料的研究得到了广泛的关注。系统综述了近年来用于CO2吸附的5种多孔炭材料,即煤/石油焦基活性炭、生物质多孔炭、炭气凝胶、金属有机骨架衍生物和碳纳米材料,以及多孔炭材料主要的4种制备方法(高温炭化与活化法、水热炭化法、溶胶-凝胶法和模板法),并重点讨论其结构与CO2吸附性能的关系;随后对多孔炭材料的孔结构和表面化学性质吸附CO2的机理进行总结。最后,提出多孔炭材料吸附CO2发展过程中尚待解决的问题,并对其未来的发展方向进行了展望。 相似文献
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中国林产品采购经理指数(PMI)作为表征林产制造业走势兴衰的晴雨表,其权重的合理性是保障指数先导性、有效性的核心条件。通过对中国林产品PMI进行分析可以发现,现行权重体系不影响其作为“行业晴雨表”的先导性功能,但是赋权方式存在优化空间。文中提出的优化的权重体系更有利于提升林产品PMI对行业经济走势的预测能力。 相似文献
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Effects of opening size and stand characteristics on light transmittance and temperature under young trembling aspen stands 总被引:2,自引:0,他引:2
While aspen (Populus tremuloides Michx.) cover provides protection from growing season radiative frosts, reduced light levels can limit growth of understory white spruce (Picea glauca (Moench) Voss). The creation of openings around individual spruce can provide more light, but the chance of radiative frost damage increases as gaps become larger. We examined the relationship between opening size and light and temperature levels in the center of circular gaps created in young aspen stands ranging from 1.8 to 8 m tall. Models for predicting transmittance as a function of sky view factor and transmittance under intact stands are presented. Results indicated a strong relationship between light levels and opening size after considering pre-treatment light levels. The relationship between opening size and temperature was weak with minimum temperatures being more affected by the height and density of the surrounding stand than opening size. 相似文献
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Nicolás Franck Sylvia Winkler Claudio Pastenes Rodrigo Infante 《Agroforestry Systems》2007,69(3):215-229
Murta (Ugni molinae Turcz.) is an evergreen shrub of the native forest understorey of southern Chile that produces berries which are consumed
in the local markets. Because of the natural adaptation of murta to growing under the shade of trees, we propose that an adequate
way of domesticating this species would be its cultivation in agroforestry systems. In order to assess the suitability of
three murta accessions from different regions in southern Chile for their cultivation in such systems, we established a trial
in which these accessions were submitted to six light transmittance levels (20%–100% of full solar irradiance) from planting
in spring to the following autumn. Optimum growth, as assessed through dry mass accumulation and emission of branches and
metamers, was achieved at moderate light transmittance levels (50%–65%). These growth traits showed stable positive responses
to the relative amount of light intercepted by the plants (as estimated from plant structural traits) up to these optimum
light transmittance levels and diverged to lower values thereafter. These stable relationships suggest that the differences
in plant growth at low and moderate light transmittance levels can be attributed to restrictions of photosynthesis by light
availability. The reduction in growth for higher light transmittance levels may be partly attributed to photoinhibition as
suggested by reduced chlorophyll content and relatively low increments in carotenoid content in leaves at high light transmittance
levels. 相似文献