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1.
强化青葙修复镉污染土壤的柠檬酸施用方式优化试验研究   总被引:2,自引:0,他引:2  
傅校锋  刘杰  龙玉梅  孟德佳 《土壤》2020,52(1):153-159
采用盆栽试验,研究了以不同时间和次数共10种方式施用柠檬酸对青葙(Celosia argentea Linn.)修复Cd污染土壤及地上部Cd积累量的影响,为筛选最佳的柠檬酸施用方式,提高青葙Cd修复效率提供参考。结果表明:所有处理的青葙各部分生物量均增加,其中移栽前施加柠檬酸,混匀后平衡两周(CA+Bal)处理可显著增加青葙各部分生物量,叶、茎、根及地上部分别比对照(CK)处理增加66.18%、67.22%、65.58%和66.92%;第4周单次施加柠檬酸(CA+4)处理和第4、5周等量分两次施加柠檬酸(CA+4/5)处理显著增加青葙地上部Cd含量,分别较CK处理增加67.18%和66.82%;CA+Bal和CA+4/5处理显著增加了青葙地上部Cd积累量,分别比CK处理高139.62%和129.59%。可见,移栽前施加柠檬酸,混匀后平衡两周和第4、5周等量分两次施加柠檬酸处理更有利于提高青葙对Cd污染土壤的修复效率。  相似文献   

2.
Detailed karyotypic studies have been done in 20 wild and cultivated taxa of Chenopodium, which belong to three ploidy levels 2x, 4x and 6x. C. quinoa (4x) shows minor but consistent differences in the arm ratio of various chromosomes within the complements of different accessions. The chromosomes can be arranged in 18 pairs that suggest allotetraploid nature. The karyotype of C. berlandieri subsp. nuttalliae (4x) is basically similar to that of C. quinoa. C. bushianum (6x) is distinctly different from the above two species in showing highest ratio between longest and shortest chromosomes. C. album complex is characterized by consisting of 2x, 4x and 6x cytotypes. Marked karyotypic differentiation is seen even among various 2x accessions. The 4x cytotype has a more asymmetrical karyotype as compared with 2x and 6x cytotypes. The karyotypic differences are also apparent between two 6x cytotypes studied. C. strictum (6x) and C. giganteum (6x) show close similarity to 6x types of C. album.  相似文献   

3.
采用盆栽试验方法,研究了污泥改良锰矿尾渣对紫茉莉(Mirabills jdapa)、青葙(Celosia argentea)、一串红(Salviasplendens)和鸡冠花(Celosia cristata)4种花卉植物生长及其富集铅、镉、锌和锰的影响。结果表明,添加污泥增加基质的EC值、总氮、总磷、总钾和有机质含量,而降低铅、镉、锌和锰含量。紫茉莉与青葙生物量、株高和根长大于一串红和鸡冠花。紫茉莉根部铅、镉和锰含量分别为3110.93~4189.16、300.28~399.16和31100.93~36809.77mg·kg-1,都远高于其地上部分含量。青葙根部铅含量超过1000mg·kg-1,而其地上部分含量少;其地上部分镉和锰含量分别为322.13~441.88和21888.54~26511.31mg·kg-1,都大于其根部含量,青葙具有镉和锰超富集植物的特性。污泥改良锰矿尾渣促进这4种花卉植物生物量、株高和根长增加。除紫茉莉锌含量外,添加污泥改良锰矿尾渣增加这4种花卉植物的铅、镉、锌和锰含量。在锰矿尾渣污染区进行植物修复时,采用紫茉莉、青葙和添加污泥强化植物修复效率可行。  相似文献   

4.
青葙修复镉污染土壤的田间试验研究   总被引:2,自引:0,他引:2  
为探索氯化铵与柠檬酸和青葙(Celosia argentea L.)联合修复重金属Cd污染土壤的方法,通过田间试验,研究了氯化铵与柠檬酸对青葙生长和吸收富集Cd的影响、根际土与非根际土之间的差异,以及对土壤Cd含量和形态的影响。结果表明,种植青葙并施加氯化铵(C+AC)处理能显著促进青葙地上部干重的提高,比只种植青葙(C)处理增产53.03%;种植青葙并施加柠檬酸(C+CA)处理能提高青葙对Cd的吸收富集能力,地上部Cd含量比C处理提高16.64%;而C+AC处理对青葙地上部Cd积累量的促进效果最佳,比C处理提高69.49%;青葙的种植均显著降低根际土Cd的酸溶态含量,C、C+AC和C+CA处理的降低幅度分别为10.31%,15.00%和12.67%,显著大于非根际土;C+CA处理的土壤Cd含量降率最大,为5.33%,而不种植青葙,不施加药剂(CK)处理为1.43%,只有C+CA处理的26.83%。因此,青葙植物提取对Cd的降低仍起关键作用,且施加柠檬酸对表层土壤Cd的修复性能最好。  相似文献   

5.
[目的]揭示断陷盆地石漠化生态修复区土壤养分、酶活性特征,筛选修复效果较好的模式,为石漠化修复治理提供科技支撑。[方法]以典型断陷盆地云南省建水县为研究区,探究4种植被修复类型(云南松+银木荷人工混交林、柏木+银木荷人工混交林、干香柏人工纯林、自然植被恢复样地)的土壤碳、氮、磷含量和化学计量特征及酶活性特征(淀粉酶、脱氢酶、葡萄糖苷酶、酸性磷酸酶、FDA水解酶、脲酶)。[结果]土壤有机碳、全氮、全磷平均质量分数分别为25.81,1.89,0.41 g/kg,有机碳、全氮含量均为自然植被恢复下最高,全磷含量为干香柏纯林下最高;自然植被恢复下土壤C∶P与N∶P最高,C∶N最高的为柏木+银木荷混交林。土壤淀粉酶活性为干香柏人工纯林最高,自然恢复植被恢复下最低,其余酶活性均为自然植被恢复下最高。相关性分析表明,土壤有机碳、全氮、全磷、pH值会对土壤酶活性产生影响,且冗余分析结果显示影响土壤酶活性的最主要养分因子为全氮,解释了72.3%的土壤酶活性变异。[结论]不同植被修复模式下土壤养分及酶活性特征不同,经18 a植被修复后,自然植被恢复和干香柏人工纯林两种修复模式下土壤养分条件较好,是该地区较...  相似文献   

6.
The distribution of total 14C residues was studied in egg yolk and albumen after administration of either single or multiple oral dosages of [14C]sulfamethazine (SMZ). One day after a single dose of [14C]SMZ (121 mg of sulfamethazine, 2.42 x 10(7) dpm), the 14C residue concentration peaked in egg albumen and egg yolk with the concentration in the former >4-fold greater than in the latter. Three days postdose, the 14C residue concentration in the yolk was approximately 7-fold higher than in the egg albumen. A multiple dose of [14C]SMZ containing sulfamethazine mass equivalent of an average therapeutic dose (282 mg, 2.9 x 10(7) dpm) for chickens was also administered orally for six consecutive days to hens. A significantly reduced level of egg production was observed during the medication, and most of the hens stopped laying eggs after the last dose. The 14C residue concentrations peaked on the last day (sixth) of medication in egg albumen and yolk. The 14C residue concentrations were also measured in liver, muscle, blood, and plasma of chickens sacrificed at 1, 24, 48, and 72 h after the last dose. Highest concentrations of 14C residue were accumulated in liver followed by, in decreasing order, blood, plasma, and muscle.  相似文献   

7.
The chemical stability and colorant properties of three betaxanthins recently identified from Celosia argentea varieties were evaluated. Lyophilized betaxanthin powders from yellow inflorescences of Celosia exhibited bright yellow color and high color purity with strong hygroscopicity. The aqueous solutions containing these betaxanthins were bright yellow in the pH range 2.2-7.0, and they were most stable at pH 5.5. The betaxanthins in a model system (buffer) were susceptible to heat, and found to be as unstable as red betacyanins (betanin and amaranthine) at high temperatures (>40 degrees C), but more stable at 40 degrees C with the exclusion of light and air. The three betaxanthins had slightly higher pigment retention than amaranthine/isoamaranthine in crude extracts at 22 degrees C, as verified by HPLC analysis. Lyophilized betaxanthins had much better storage stability (mean 95.0% pigment retention) than corresponding aqueous solutions (14.8%) at 22 degrees C after 20 weeks. Refrigeration (4 degrees C) significantly increased pigment retention of aqueous betaxanthins to 75.5%.  相似文献   

8.
The ellagic acid, total phenolic, and vitamin C contents in four raspberry cultivars (Heritage, Autumn Bliss, Rubi, and Zeva) grown in Spain were detected and quantified by HPLC in fresh, just frozen, and stored fruits at -20 degrees C for a one year period. Ellagic acid [207-244 mg kg(-)(1) of fresh weight (fw)], total phenolic (137-1776 mg kg(-)(1) of fw), and vitamin C (221-312 mg kg(-)(1) of fw) contents in raw material were higher in the late cultivars Zeva and Rubi than in the early cultivars Autumn Bliss and Heritage. The freezing process slightly affected the values of extracted ellagic acid, total phenolic, and vitamin C content. At the end of long-term frozen storage (12 months), no significant change of total phenolic content extracted was observed, but significant decreases of 14-21% in ellagic acid and of 33-55% in vitamin C were quantified. Free radical scavenging capacity measured as antiradical efficiency (AE) depends on the seasonal period of harvest. Late cultivars, Rubi (6.1 x 10(-)(4)) and Zeva (10.17 x 10(-)(4)), showed higher AE than early cultivars, Heritage (4.02 x 10(-)(4)) and Autumn Bliss (4.36 x 10(-)(4)). The freezing process produced a decrease of AE values in the four cultivars ranging between 4 and 26%. During the frozen storage, the AE values reached after the freezing process remained unchanged.  相似文献   

9.
黄土高原高塬沟壑区坡面表层土壤水分研究   总被引:6,自引:2,他引:4       下载免费PDF全文
在黄土高寒区,通过人工控制土壤水分的方法,利用Li-6400便携式光合测定系统对银水牛果和沙棘苗木叶片的气体交换参数因子的光响应进行研究。结果表明,2种灌木的净光合速率、蒸腾速率、水分利用效率随着光强的增大而增强,而随着光强进一步增大,净光合速率、水分利用效率却出现下降的趋势,蒸腾速率仍继续增大。随着土壤含水量的增加,2种灌木的净光合速率、蒸腾速率、气孔导度、胞间CO2浓度逐渐增大,水分利用效率先上升,在轻度水分胁迫下达到最大值后下降。在相同土壤含水量下,银水牛果光补偿点明显小于沙棘,光饱和点大于沙棘,光能利用率高于沙棘;银水牛果的表观量子效率大于沙棘,在低光强下的光合能力较高。在水分胁迫下,2种灌木相比,沙棘比银水牛果更易受强光胁迫;银水牛果对弱光的利用能力高于沙棘,水分利用效率高于沙棘,耐旱生产力较高。  相似文献   

10.
In modern agricultural systems employing conservation tillage practices, glyphosate is widely used as a preplant burndown herbicide in a wide range of crops. Conservation tillage systems are characterized by a significant presence of crop residues at the soil surface so that glyphosate is applied to a soil matrix rich in poorly decomposed crop residues. Incorporation of corn residues in the range from 0.5 to 4% caused different effects on mineralization and sorption of [14C]glyphosate in sandy and sandy loam soils. More specifically, low levels of incorporated corn residues did not affect or slightly stimulated herbicide mineralization in the sandy and sandy loam soils, respectively. In the sandy soil, incorporation of the highest level of corn residues (4%) caused a decrease in [14C]glyphosate mineralization. [14C]Glyphosate sorption on both soil types was reduced in samples receiving high amounts of incorporated corn residues.  相似文献   

11.
Molecular and morphological variation of six perennial and five annual species including domesticated chickpea, C. arietinum, were inferred on the basis of RAPD and S.E.M. seed coat features using three outgroup taxa (Lens ervoides, Lathyrus japonica and Pisum sativum). Of the 66 decamer arbitrary primers tested, eight primers revealed 87 informative fragments. Neighbor-joining cluster analysis using Jaccard's coefficient of similarity on the basis of polymorphic fragments indicated a narrow variation in C. arietinum and recognized two major clusters in the genus Cicer. The first one included four species of sect. Monocicer: C. echinospermum, C. arietinum, C. reticulatum and Iranian material of C. bijugum. The second cluster contained annual and perennial species belonging to sections Chamaecicer, Polycicer and Acanthocicer. The character state of morphological and ecological traits on the RAPD phenogram indicated no monophyletic incision. Our results suggested that the high genetic difference between annual and perennial species might be regarded as a rapid speciation of section Monocicer. Reconsideration of traditional classification of sections Polycicer and Acanthocicer is necessary. The Desi and Kabuli types of C. arietinum could not be separately grouped at the DNA level, and the low level of genetic variation of C. arietinum may result from a bottleneck during the domestication process.  相似文献   

12.
本文对14 C 绿磺隆在 7种不同类型土壤中形成结合残留 ( 14 C ER)、可提态残留( 14 C ER)以及矿化为14 C CO2 的规律、影响14 C BR的主要因子及其在腐殖质中的分布规律等进行了研究。结果表明 :( 1 ) 14 C 绿磺隆在土壤中形成的14 C ER含量与土壤pH呈显著正相关 ,与土壤粘粒和有机质含量呈显著负相关 ,14 C ER中的绿磺隆母体化合物的消减满足一级反应动力学方程 ,其在 7种土壤中的半减期分别为 1 3 0~ 1 3 3 3d。pH是影响绿磺隆母体化合物降解的主要因子 ;( 2 ) 14 C 绿磺隆在 7种土壤中的14 C BR含量与土壤pH呈显著负相关 ,并与土壤粘粒含量呈显著正相关 ,土壤pH是14 C 绿磺隆在土壤中形成BR的主要影响因子 ;( 3 ) 14 C 绿磺隆形成的14 C BR主要分布在富啡酸和胡敏素中 ;14 C BR分布在胡敏酸中的相对百分比约为 2 % ,在14 C 绿磺隆BR的形成过程中 ,富啡酸的作用 >胡敏素 胡敏酸 ;( 4) 14 C 绿磺隆在 7种土壤中的14 C BR含量 ,在培养 2 0d内均随时间而快速增加 ,2 0d后变化量较小。 7种土壤中的14 C BR含量最大值分别占引入量的 53 5%、40 9%、3 7 8%、1 6 4%、42 5%、41 0 %和 3 1 3 % ;( 5)培养 90d内 ,14 C 绿磺隆通过三嗪杂环开环矿化为14 CO2 的量约占引入量的 4%~9% ,而土壤 1表明14 C  相似文献   

13.
The antioxidant activity of the water extract of Tilia argentea Desf ex DC was determined by the thiocyanate method. The antioxidant activity of the water extract increased with the increasing amount of lyophilized extract (50-400 microg) added into the linoleic acid emulsion. Statistically significant effect was determined in 100 microg and higher amounts. Antioxidant activities of water extracts of tilia (Tilia argentea Desf ex DC), sage (Salvia triloba L.), and two Turkish black teas commercially called Rize tea and young shoot tea (Camellia sinensis) were compared. For comparison studies, 100 microg portions of extracts were added into test samples. All samples were able to show statistically significant antioxidant effect. Both of the tea extracts showed highest antioxidant activities, nevertheless, differences between tilia and sage and tilia and tea were not statistically significant (for both cases p > 0.05). Like antioxidant activity, the reducing power of water extract of Tilia argentea Desf ex DC was also concentration dependent. Even in the presence of 50 microg of extract, the reducing power was significantly higher than that of the control (p < 0.05) in which there was no extract. Unlike antioxidant activity, the highest reducing power activity was shown by sage extract. Among the tea extracts, young shoot extract was the most effective one, however, it had significantly lower activity than sage (p < 0.05). Although tea flower had the lowest reducing power activity, it was higher than that of tilia. But this difference was not statistically significant (p > 0.05). From these results, we could suggest that although the reducing power of a substance may be an indicator of its potential antioxidant activity, there may not always be a linear correlation between these two activities. In addition, antimicrobial activities of each of the above extracts were studied by disk diffusion methods on different test microorganisms. None of the extracts showed antibacterial activity on the studied microorganisms.  相似文献   

14.
《Pedobiologia》2014,57(4-6):257-262
Many studies have shown the effects of aboveground plant species on soil organisms due to differences in litter quality. However, the calcium concentration in soil has received less attention as a controlling factor of soil invertebrate communities, even though it is an essential element for many animals, especially crustaceans. Litter of Japanese cedar (Cryptomeria japonica) plantations, which account for 19% of the forested area in Japan, has a higher calcium concentration compared to other taxa such as broad-leaved trees. We predicted that C. japonica plantations affect soil invertebrates by altering calcium availability. We compared soil properties including exchangeable calcium concentration and soil invertebrate communities between C. japonica plantations and natural broad-leaved forests. Exchangeable calcium was significantly higher in soil from cedar plantations than in that from broad-leaved forests. The invertebrate community composition differed between the two forest types and was best explained by the exchangeable calcium concentration. In particular, two major taxa of soil crustaceans (Talitridae and Ligidium japonicum) were found only in cedar plantations. Our results suggest that calcium concentrations in soil are altered in C. japonica plantations and that this affects soil invertebrate communities.  相似文献   

15.
We evaluated morphological, isozyme and biochemical diversity of a total of 87 accessions in the genus Camellia [Camellia sinensis var. sinensis (10), C. talinensis (7), C. sinensis var. dehungensis (3), C. crassicolumna (3) and C. sinensis var. assamica (64)]. Great variation of morphological characters was apparent within each taxa. Across the five taxa, all leaf and most flower quantitative characters showed significant differences while all fruit quantitative characters measured did not differ significantly, and, seven (i.e., life form, bud color, petal texture, pubescence on ovary, style number, stamen location and locule per fruit) of the 33 qualitative characters yield significant differences. As a whole, caffeine content had the highest variation with CV of 22.7%, water extract solid showed the least variation (13.4%) and content of polyphenols (20.0%) and free amino acids (18.8%) showed intermediate variations. Camellia taliensis and C. sinensis var. assamica had significantly higher content of polyphenols and water extract solid than in the other three taxa, while no significant differences were detected for the content of caffeine and free amino acids. For allozyme study, 14 loci presented good resolution, among which, nine loci (64%) were polymorphic in each taxon (AAT-3, FUM-1, 6PDG-1, G6PDH-1, G3PDH-1, ME-1, PGM-1, PGM-2 and SKD-1). The percentage of polymorphic loci (P) for each taxon was 21.4–50.0%. Mean heterozygosity per locus (H e ) varied 0.114–0.218. F ST value indicated that only 4.6% of the variations could be ascribable to genetic differences among taxa. Genetic relationships among the five taxa revealed by allozymes, were also exposed by the result of clustering of the morphological and biochemical characters.  相似文献   

16.
Triple staining with the fluorochromes chromomycin A3, distamycin A and 4′-6-diamidino-2-phenylindole (CMA/DA/DAPI) was applied to somatic metaphases and interphase nuclei of 11 taxa of wild chili peppers (Capsicum), with 2n = 2x = 24 (C. annuum var. glabriusculum, C. cardenasii, C. chacoense, C. flexuosum, C. galapagoense, C. eximium, C. praetermissum and C. tovarii) and 2n = 2x = 26 (C. recurvatum, C. rhomboideum and C. villosum) to analyse heterochromatin type, amount and distribution in wild members of this genus. Heterochromatic banding patterns allowed the identification of all the taxa examined and contributed to their taxonomic grouping. GC-rich heterochromatin (CMA+/DAPI?) was typical in all taxa; only C. praetermissum possessed also AT-rich (CMA?/DAPI+) and mixed GC- and AT-rich (CMA+/DAPI+) bands. Heterochromatin amount (expressed as % of karyotype length) ranged between 1.72 (C. chacoense) and 16.82 (C. flexuosum) and was positively correlated with karyotype length in most of the taxa examined. Heterochromatin located mainly at terminal position of chromosomes but intercalary position prevailed in C. flexuosum. Nucleolus organizer regions (NOR)-associated GC-rich heterochromatin was exclusively terminal and included the distal macrosatellite and a small portion on the corresponding arm. In all the taxa analysed, an equilocal heterochromatin distribution in non-homologous chromosomes of karyotype was observed, suggesting concerted evolution of heterochromatin dispersion in Capsicum.  相似文献   

17.
V. sativa L. sensu strictu was introduced in Rio Grande do Sul (Southern Brazil) by Italian settlers and is nowadays cultivated for forage in backyards and fields. V. angustifolia L. is a widespread highly polymorphic naturalized ruderal. Both taxa grow sympatrically and morphologically transitional types are often found. We analyzed 37 accessions of V. sativa, V. angustifolia, intermediate types, and V. cordata Wulf. ex. Hoppe from Rio Grande do Sul, for karyotypes, corolla and legume color, seed color pattern, pod constrictions and phenological development. Karyotypes allowed a clear distinction between the taxa: V. sativa (2n=12) had a metacentric marker chromosome, V. angustifolia and intermediate types (2n=12) only acrocentric chromosomes and V. cordata presented 2n=10. Flowering and fruiting periods were shorter for V. sativa than for V. angustifolia, which was separated into early and late flowering groups. Qualitative characters separated the taxa by Jaccard coefficients into three groups: V. sativa at 0.5 similarity level, V. angustifolia and intermediate types at 0.6 and V. cordata joined V. sativa at 0.12. Our results show that karyotypes and a range of qualitative characters are diagnostic for the aggregate represented in Southern Brazil.  相似文献   

18.
o-Nitrophenol, released from o-nitrophenyl-beta-D-galactopyranose as catalyzed by beta-galactosidase, a tetramer of Escherichia coli, has been exploited for the detection of E. coli contamination in foodstuffs. This reaction was detected using a boron doped diamond electrode poised at +0.93 V, without any surface modification. The enzyme was effectively induced by isopropyl-beta-D-thiogalacto-pyranoside with the maximum enzyme activity observed with sodium dodecyl sulfate at 50 degrees C. A biphasic calibration plot was observed: 4 x 10(4) to 2 x 10(5) cells/mL and 2 x 10(5) to 6 x 10(6) cells/mL for the first and second region, respectively. The detection limit was 4 x 10(4) cells/mL with a total analysis time of <1.5 h. Electrode fouling was easily overcome by approximately 40 rapid CV scans, and the method was applicable for detecting E. coli in artificially spiked samples of beef, pork, chicken, milk, and tap water.  相似文献   

19.
Summary Hibiscus L. section Furcaria DC. (Malvaceae) is a natural group of more than 100 known species, many of which are handsome ornamentals with large, showy, delicate flowers. This group includes the fiber, food, and medicinal plants kenaf, H. cannabinus L., and roselle, H. sabdariffa L. The basic chromosome number is x = 18. In nature are found diploid, tetraploid, hexaploid, octoploid, and decaploid taxa. This group displays a remarkable amount of genome diversity, as shown by cytological analysis of 140 hybrid combinations from over 60,000 crosses. At least 13 genomes are present: A, B, C, D, E, G, H, J, P, R, V, X, and Y. Subsaharan Africa is the center of genome diversity; nine of the 13 genomes are represented in African taxa, and nine of the 10 confirmed diploid species occur in Africa. Five (possibly six) genomes reside in extant diploids. The G genome (or a modified G genome) is widely distributed. Found in only one diploid species in Africa, it is found also in African tetraploid and African and Indian octoploid species, in New World tetraploid and decaploid species, and in Australian hexaploid species. The G-genome apparently was widely dispersed and differentiated, followed by hybridization, subsequent chromosome doubling, and radiation. The A, B, X, and Y genomes, on the other hand, are confined mainly to Africa, with a few taxa in Asia, and apparently are the products of a later round of hybridization and allopolyploidy.  相似文献   

20.
The replacement of indigenous vegetation by commercial alien plantations can strongly affect invertebrate species richness and composition. This has been demonstrated for some invertebrate taxa in the Fynbos vegetation of the Western Cape, but the response of litter-dwelling Collembola has not been documented. Here we compared the richness and abundance of litter-dwelling Collembola assemblages in Pinus radiata plantations and in adjacent rehabilitated Cape Flats Sand Fynbos (CFSF) in the Tokai Forest Reserve, Western Cape, South Africa. A total of 48 Collembola morphospecies was identified, of which 14 species were recorded exclusively from the pine plantations, and six exclusively from the CFSF. Abundance and morphospecies richness was higher in the pine plantations than in the CFSF, with 34 morphospecies found in the CFSF, and 42 morphospecies in the pine plantations. Fourteen species were likely invasive taxa, of which eight were found in CFSF, and 13 in the pine plantations. Multivariate analyses indicated significant differences in the assemblages of the two habitat types based on abundance, but using presence–absence data only, no difference was found. Significant relationships were found between springtail richness and abundance and site humidity, which differed between the CFSF (drier) and pine plantation (moister), likely accounting for the differences in richness and abundance among habitat types.  相似文献   

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