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1.
Takeshi Takasaki Kazuma Okada Carlos Castillo Yuki Moriya Toshihiro Saito Yutaka Sawamura Naoko Norioka Shigemi Norioka Tetsu Nakanishi 《Euphytica》2004,135(2):157-167
A new S
9-allele was discovered in 6 Japanese pear cultivars, ‘Shinkou’, ‘Shinsei’, ‘Niitaka’, ‘Amanogawa’, ‘Nangetsu’ and ‘Nansui’.
cDNA encoding S
9-RNase, a stylar product of S
9-allele, was cloned from pistils of ‘Shinkou’ and ‘Shinsei’ by 3' and 5' RACE. The S
9-RNase gene had an open reading frame of 684 nucleotides encoding 228 amino acid residues. S
9-RNase had a hypervariable (HV) region different from S
1- to S
8-RNase and shared higher similarity (95.2%) with apple S
3-RNase than with 8 Japanese pear S-RNases (from 61.0% to 70.7%). Genomic PCR with primers ‘FTQQYQ’ and ‘anti-(I/T) IWPNV’ provided S
1- to S
9-amplicon (product), but could not discriminate the S
2 from the S
9 of ca. 1.3 kb. The S
2 and S
9 were distinguished by digestion with AflII and BstBI, respectively. The digestion with nine S-allele-specific restriction endonucleases, SfcI, AflII, PpuMI, NdeI,AlwNI, HincII, AccII, NruI and BstBI, distinguished S
1 to S
9, establishing that this PCR-RFLP system is useful for S-genotype assignments in Japanese pear harboring S
1- to S
9-allele. ‘Shinkou’, ‘Shinsei’, ‘Nangetsu’ and ‘Nansui’ assigned as S
4
S
9 were determined to be cross incompatible.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
2.
Summary
Valsa canker is the most serious wood rot disease of apple in Japan. Dormant, excised twig assay in vitro was used to determine
resistance to Valsa canker. ‘Fuji’, ‘Starking Delicious’, ‘M.26 EMLA’, and ‘MM.106’ were considered to be highly susceptible,
while Malus sieboldii showed moderate resistance. Several cultivars belonging toM. prunifolia, M. baccata, andM. × domestica showed comparative resistance. The phloridzin content in the bark ofMalus species was studied in relation toValsa canker resistance. Phloridzin content ofM. sieboldii was lower than inM. × domestica. Comparatively resistant cultivars had similar or higher phloridzin content compared to susceptible cultivars. No clear correlations
were found between resistance and phloridzin contents in the bark ofMalus species. 相似文献
3.
Shigeki Moriya Hiroshi Iwanami Kazuma Okada Toshiya Yamamoto Kazuyuki Abe 《Euphytica》2011,177(1):135-150
Differentiation of cultivars with simple sequence repeat (SSR) markers is a very useful technique for the true-to-type characterization
of cultivars and clarification of parent-offspring relationships. We developed an SSR marker set for cultivar identification
comprising 15 markers that were screened from 46 previously published SSRs. This marker set could be used for apple varieties
including Malus × domestica and/or other Malus species. These SSRs successfully characterized 95 apples, including the leading and major founding cultivars used worldwide
for modern apple breeding. Therefore, this marker set could be applied to almost all apple cultivars. We also analyzed the
parent-offspring relationships of 69 cultivars by considering allele transmissions. This analysis revealed the true parentage
of the following seven cultivars: ‘Kizashi’, ‘Chinatsu’, ‘Honey Queen’, ‘Haruka’, ‘Seirin’, ‘Ozenokurenai’, and Morioka #48.
This analysis also revealed a parentage discrepancy for ‘Hacnine’. From the parent-offspring analysis, two microsatellite
mutation events at alleles inherited from pollen parents were observed. 相似文献
4.
Ana Delia Gisbert Carlos Romero José Martínez-Calvo Carmen Leida Gerardo Llácer María Luisa Badenes 《Euphytica》2009,168(1):121-134
Loquat (Eriobotrya japonica (Thunb.) Lindl.) is a minor Rosaceae fruit of growing interest as an alternative to the main fruit crops. In this context,
the selection of new cultivars to satisfy the market demand will request the suitable characterization of the available germplasm.
In this work, genetic relationships among 83 loquat accessions from different countries belonging to the European loquat germplasm
collection, held at the Instituto Valenciano de Investigaciones Agrarias (IVIA) in Moncada (Spain) were evaluated using microsatellites
and S-allele fragments. A total of nine single sequence repeats (SSRs) from Malus and Eriobotrya genera revealed 53 informative alleles and the S-RNases consensus primers detected 11 self-incompatibility putative alleles. The combined data allow to distinguish unambiguously
80 out of the 83 accessions studied. Unweighted pair-group method (UPGMA) cluster and principal coordinates analysis (PCoA),
based on Dice’s genetic distance, generally grouped genotypes according to their geographic origins and pedigrees. Discrepancies
and similarities of the results obtained with other variability analysis, based on pomological traits or molecular markers,
on the same loquat collection are discussed. 相似文献
5.
Molecular markers linked to the <Emphasis Type="Italic">fin</Emphasis> gene controlling determinate growth habit in common bean 总被引:1,自引:1,他引:0
Astrid Pañeda Cristina Rodríguez-Suárez Ana Campa Juan José Ferreira Ramón Giraldez 《Euphytica》2008,160(2):241-248
The S-genotypes of 16 apricot (Prunus armeniaca L.) cultivars native to China were determined by the S-allele PCR approach and the results were confirmed by cross-pollination tests among these cultivars. Primer combination EM-PC2consFD + EM-PC3consR,
based on the conserved regions C2 and C3 of Rosaceous
S-RNase genes, was the most useful primer combination for identifying Chinese apricot S-alleles. Twelve S-RNase alleles were identified using this primer combination, and they were defined as follows: S
9 was 657 bp, S
10 was 266 bp, S
11 was 464 bp, S
12 was 360 bp, S
13 was 401 bp, S
14 was 492 bp, S
15 was 469 bp, S
16 was 481 bp, S
17 was 487 bp, S
18 was 1337 bp, S
19 was 546 bp and S
20 was 1934 bp. S
11–S
20 were new S-RNase genes deposited in GenBank under accession numbers DQ868316, DQ870628-DQ870634, EF133689 and EF160078, respectively.
Our findings contribute to a more efficient breeding program of Chinese apricot and further studies on the S-RNase genes. 相似文献
6.
Characterization of segregation distortion on chromosome 3 induced in wide hybridization between indica and japonica type rice varieties 总被引:6,自引:0,他引:6
S. Matsushita T. Iseki Y. Fukuta E. Araki S. Kobayashi M. Osaki M. Yamagishi 《Euphytica》2003,134(1):27-32
We previously surveyed chromosomal regions showing segregation distortion of RFLP markers in the F2 population from the cross between a japonica type variety ‘Nipponbare’ and an indica type variety ‘Milyang23’, and showed
that the most skewed segregation appeared on the short arm of chromosome 3. By comparison with the marker loci where distortion
factors were previously identified, this region was assumed to be a gametophytic selection-2 (ga2) gene region. To evaluate this region, two near isogenic lines (NILs) were developed. One NIL had the ‘Nipponbare’ segment
of this region on the genetic background of ‘Milyang23’ (NIL9-23), and the other NIL had the ‘Milyang23’ segment on the genetic
background of ‘Nipponbare’ (NIL33-18). NIL9-23 and ‘Milyang23’, NIL33-18 and
‘Nipponbare’, and ‘Nipponbare’ and ‘Milyang23’ were respectively crossed to produce F1 and F2 populations. The F1 plants of NIL9-23 × ‘Milyang23’ and NIL33-18 × ‘Nipponbare’ showed high seed fertility and the same pollen fertility as their
parental cultivars, indicating that ga2 does not reduce seed and pollen fertility. Segregation ratio of a molecular marker on the ga2 region in the three F2 populations was investigated to clarify whether segregation distortion occurred on the different genetic backgrounds. Segregation
distortion of the ga2 region appeared in the both F2 populations from the NIL9-23 and ‘Milyang23’ cross (background was
‘Milyang23’ homozygote) and the ‘Nipponbare’ and ‘Milyang23’ cross (background was heterozygote), but did notin the F2 population from the NIL33-18 and ‘Nipponbare’ cross (background was ‘Nipponbare’ homozygote). This result indicates that
ga2 interacts with a ‘Milyang23’ allele(s) on the different chromosomal region(s) to cause skewed segregation of the ga2 region. In addition, segregation ratio was the same between the F2 populations from NIL9-23 × ‘Milyang23’ and ‘Nipponbare’ × ‘Milyang23’ crosses, suggesting that the both genotypes, ‘Milyang23’
homozygote and heterozygote, of gene(s) located on the different chromosomal region(s) have the same effect on the segregation
distortion.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
7.
Epidemiological field controls in different Italian locations and seedling evaluations of the ‘Thatcher’ near-isogenic lines
(NILs) carrying the leaf rust resistance genes Lr1, Lr9, Lr24 and Lr47 were conducted during 5 years of testing. These genes confirmed their effectiveness in both field and greenhouse conditions.
Moreover a backcross program was carried out by using as recurrent parents the susceptible high-quality common wheat cvs ‘Bolero’,
‘Colfiorito’, ‘Serio’ and ‘Spada’ and the ‘Thatcher’ NILs carrying the above mentioned genes as donor parents. The progenies
of different cross combinations were selected by both resistance tests and marker assisted selection using molecular markers
(STS, SCAR, CAPS) closely linked to Lr genes: a complete cosegregation was observed between the resistance genes used and the corresponding molecular markers. 相似文献
8.
Self and cross-incompatibility determination by means of fruit and seed set experiments or pollen tube growth observations
in the style has been frequently reported to be unclear in pear (Pyrus communis L.). Thus,in order to develop a reliable in vivo method to test pollen-pistil incompatibility in pear, pollen tube performance has been studied along the pistil following
self and cross-pollinations. Results show that, while pollen tube growth in the style may be an unclear test, ovule observation
at the microscope for the presence of pollen tube in the nucellus is a proper method to test incompatibility in this crop.
With this analysis we could identify S-alleles of ‘Williams’ (S1S2) and ‘Coscia’(S3S4), and three of the four possible S-genotypes resulting from the ‘Williams’ × ‘Coscia’ cross, as represented by ‘Butirra Precoz
Morettini’ (S1S3), ‘Santa Maria Morettini’ (S2S3)and ‘Tosca’ (S1S4). This result demonstrates that ‘Williams’ and ‘Coscia’ cultivars do not share any allele in common. We also established
two new inter-incompatibility groups in pear. Furthermore, the presence of a common allele between ‘Williams’ and ‘Agua de
Aranjuez’,and ‘Coscia’ and ‘Agua de Aranjuez’, three apparently unrelated old cultivars, may indicate a narrower genetic base
than expected for European pear. This finding together with the fact that 40% of new released cultivars have direct or indirect
parental relationship with the cultivars ‘Coscia’ and/or ‘Williams’, anticipates the possibility of new cases of cross-incompatibility
for this crop in the future. Both the method described and the determination of the S-genotypes will facilitate the characterisation
of self and cross-incompatibility relationships in this species.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
9.
The inheritance of the resistance to Fusarium oxysporum f. sp. melonis (F.o.m.) races 0 and 2 in ‘Tortuga’, a Spanish cantalupensis accession, was studied from crosses of ‘Tortuga’ by the susceptible line ‘Piel de Sapo’ and the resistant one ‘Charentais-Fom1’
that carries the resistance gene Fom-1. The segregation patterns observed in the F2 (‘Tortuga’ × ‘Piel de Sapo’) and the backcross (‘Piel de Sapo’ × (‘Tortuga’ × ‘Piel de Sapo’) populations, suggest that resistance
of ‘Tortuga’ to races 0 and 2 of F.o.m. is conferred by two independent genes: one dominant and the other recessive. In the F2 derived from the cross between accessions
‘Tortuga’ and ‘Charentais-Fom1’, the lack of susceptible plants indicated that the two accessions are carrying the same resistance
gene (Fom-1). The analysis of 158 F2 plants (‘Tortuga’ × ‘Piel de Sapo’) with a Cleaved Amplified Polymorphic Sequence marker 618-CAPS, tightly linked to Fom-1 (0.9 cM), confirmed that ‘Tortuga’ also carries a recessive gene, that we propose to symbolize by fom-4. 相似文献
10.
John L. Norelli Herb S. Aldwinckle Luis Destéfano-Beltrán Jesse M. Jaynes 《Euphytica》1994,77(1-2):123-128
Summary Apple (Malus domestica) transgenic T1 was obtained byAgrobacterium tumefaciens-mediated transformation of Malling 26 rootstock using the plasmid binary vector pLDB 15. pLDB 15 contains within its T -DNA
a gene encoding the lytic protein attacin E. The integration of the attacin E gene into the apple genome was confirmed by
Southern analysis. Northern analysis indicated the presence of an attacin E mRNA in plants inoculated withErwinia amylovora. After inoculation ofin vitro grown plants of T1, Malling 26, and Malling 7 (resistant control) withE. amylovora, the loglo of the inoculum concentration lethal to 50% of the plants was 5.4, 4.4, and 5.6, respectively. In greenhouse trials
for resistance to fire blight, T1 was significantly more resistant than ‘Mailing 26’. 相似文献
11.
Hela Chikh-Rouhou Rafael González-Torres Ali Oumouloud José M. Alvarez 《Euphytica》2011,182(2):177-186
The resistance to Fusarium oxysporum f.sp. melonis (Fom) race 1.2 has been studied in melons, such as the Portuguese accession ‘BG-5384’ and in the Japanese ‘Shiro Uri Okayama’,
‘Kogane Nashi Makuwa’, and ‘C-211’, since a good characterization of the resistance is necessary before its introgression
into commercial varieties. These four melon accessions showed a high level of resistance to races 0, 1, and 2 of Fom, indicating that the partial resistance to the race 1.2 previously detected may not have been race specific. To determine
the mode of inheritance of the resistance to Fom race 1.2, the F1, F2, BCPR, and BCPS generations from the crosses between the four resistant accessions above and ‘Piel de Sapo’, a Fom race 1.2 susceptible melon, were developed. They were subsequently inoculated with two Fom isolates, one from the pathotype 1.2Y and the other from the pathotype 1.2W. The area under the disease progress curve was
determined for each inoculated plant, and the data were analyzed. We show that the resistance seen in these accessions is
polygenically inherited with a complex genetic control because many epistatic interactions were detected. The three epistatic
effects; additivity × additivity, dominance × dominance, and dominance × additivity are present and significant, with differing
magnitudes from one cross to the next. The relatively low heritabilities, and these epistatic effects make difficult the improvement
of the resistance, from these sources, through a standard selection procedure. 相似文献
12.
Morphological and molecular characterization of melon accessions resistant to Fusarium wilts 总被引:1,自引:1,他引:0
Fusarium wilt incited by Fusarium oxysporum f. sp. melonis (F.o.m) is one of the most widespread and devastating melon diseases. While resistance to physiological races 0, 1, and 2
is relatively frequent in different botanical varieties, sources of resistance to race 1,2 are restricted to a few Far-Eastern
accessions. In this work, the results of a screening for resistance to F.o.m. race 1,2 among 32 accessions are presented.
Three Japanese accessions (‘Kogane Nashi Makuwa’, ‘C-211’, and ‘C-40’) showed the highest resistance levels, but useful levels
of resistance were also detected in one Russian ‘C-160’ and two Spanish (‘C-300’ and ‘Mollerusa-7’) accessions. These resistant
materials, together with other accessions previously described as resistant to F.o.m. races 0, 1, and/or 2 have been morphologically
and molecularly characterized. Based on cluster analysis, these accessions have been grouped according to the botanical subspecies
they belong to. Assessment of genetic diversity indicated that the resistant accessions to races 0, 1 and 2, are scattered
along the established clusters. On the other hand, high levels of resistance to the race 1,2 could be found only among accessions
belonging to Cucumis melo subsp. agrestis, nevertheless, a certain degree of resistance to this race could also be found within some accessions belonging to subsp.
melo. As far as we know, this is the first report of resistance to F.o.m race 1,2 found out from the Far-Eastern melon material.
Based on fruits characteristics, it appears that several inodurus and cantalupensis accessions could be exploited in breeding programs as resistance sources to F.o.m races 0, 1 and/or 2 for the improvement
of these melon types. The accessions with the highest levels of resistance to the race 1,2 appeared to be very distant both
molecularly and morphologically from the commercial types. Nevertheless ‘C-160’, ‘C-300’, and ‘Mollerusa-7’ classified as
var. inodorus are morphologically very similar to the Spanish commercial types and might be used as resistant sources in breeding these
melon types. 相似文献
13.
In a previously made cross Brassica napus cv. Oro (2n = 38) × Capsella bursa-pastoris (2n = 4x = 32), one F1 hybrid with 2n = 38 was totally male sterile. The hybrid contained no complete chromosomes from C. bursa-pastoris, but some specific AFLP (amplified fragment length polymorphism) bands of C. bursa-pastoris were detected. The hybrid was morphologically quite similar to ‘Oro’ except for smaller flowers with rudimentary stamens
but normal pistils, and showed good seed-set after pollination by ‘Oro’ and other B. napus cultivars. The fertility segregation ratios (3:1, 1:1) in its progenies indicated that the male sterility was controlled
by a single recessive gene. In the pollen mother cells of the male sterile hybrid, chromosome pairing and segregation were
normal. Histological sectioning of its anthers showed that the tapetum was multiple layers and was hypertrophic from the stage
of sporogenic cells, and that the tetrads were compressed by the vacuolated and disaggregated tapetum and no mature pollen
grains were formed in anther sacs, thus resulting in male sterility. The possible mechanisms for the production of the male
sterile hybrid and its potential in breeding are discussed. 相似文献
14.
Development of molecular markers linked to the <Emphasis Type="Italic">Fom-1</Emphasis> locus for resistance to Fusarium race 2 in melon 总被引:2,自引:2,他引:0
Ali Oumouloud Maria Soledad Arnedo-Andres Rafael Gonzalez-Torres Jose María Alvarez 《Euphytica》2008,164(2):347-356
Fusarium wilt, caused by Fusarium oxysporum f. sp. melonis (F.o.m), is a worldwide soil-borne disease of melon (Cucumis melo L.). The most effective control measure available is the use of resistant varieties. Resistance to races 0 and 2 of this
fungal pathogen is conditioned by the dominant gene Fom-1. An F2 population derived from the ‘Charentais-Fom1’ × ‘TRG-1551’ cross was used in combination with bulked segregant analysis utilizing
the random amplified polymorphic DNA (RAPD) markers, in order to develop molecular markers linked to the locus Fom-1. Four hundred decamer primers were screened to identify three RAPD markers (B17649, V01578, and V061092) linked to Fom-1 locus. Fragments amplified by primers B17649 and V01578 were linked in coupling phase to Fom1, at 3.5 and 4 cM respectively, whereas V061092 marker was linked in repulsion to the same dominant resistant allele at 15.1 cM from the Fom-1 locus. These RAPDs were cloned and sequenced in order to design primers that would amplify only the target fragment. The
derived sequence characterized amplified region (SCAR) markers SB17645 and SV01574 (645 and 574 bp, respectively) were present only in the resistant parent. The SV061092 marker amplified a band of 1092 bp only in the susceptible parent. These markers are more universal than the CAPS markers
developed by Brotman et al. (Theor Appl Genet 10:337–345, 2005). The analysis of 24 melon accessions, representing several melon types, with these markers revealed that different melon
types behaved differently with the developed markers supporting the theory of multiple, independent origins of resistance
to races 0 and 2 of F.o.m. 相似文献
15.
The quantity and quality of pollen produced by a genotype are important attributes to be considered in the selection of male
parents for breeding. The objective of this research was to determine the quantity and quality of pollen in 13 selected East
African Highland bananas (EAHB) in relation to six diploids commonly used as male parents. Pollen quantity was scored on a
scale of 0–6 while the variation of pollen stainability along the rachis was determined using acetocarmine glycerol jelly.
Actual male fertility was determined by using pollen from the EAHB varieties on M. acuminata spp. burmannicoides, ‘Calcutta 4’, as the female. The diameter of 200 viable pollen grains was measured under a microscope with the aid of a
graduated eyepiece. Significant differences were obtained for mean pollen stainability and pollen diameter. The pollen stainability
for diploids was higher than that for the EAHB. Node numbers contributed significantly to variation of pollen stainability
in EAHB (P ≤ 0.01) and ‘Pisang lilin’ (P ≤ 0.001). ‘Pisang lilin’ and the EAHB varieties had about 100 nodes as opposed to about 200 in other diploids. The EAHB varieties
produced less pollen than most of the diploids. The actual male fertility of EAHB varieties was low (1 seed/bunch), compared
to that of ‘Calcutta 4’ (223.3 seeds/bunch) and ‘Pisang lilin’ (13.7 seeds/bunch). Although the production of 2n pollen in EAHB varieties was low (0–7%), the potential exists for using them in new breeding approaches. 相似文献
16.
Hoy-Taek Kim Yutaka Hirata Yong-Uk Shin Hae-Sung Hwang Jeong-Hwan Hwang Il-Sheob Shin Dae-Il Kim Sang-Jo Kang Hyun-Jung Kim Dong-Young Shin Ill-Sup Nou 《Euphytica》2004,138(1):73-80
The pear cultivar ‘Osa-Nijisseiki’ (S2Ssm
4; sm = stylar-part mutant) has been used as a parent to breed self-compatible cultivars that produce excellent fruits. However,
determination of the self-compatibility of ‘Osa-Nijisseiki’ offspring requires a lot of time, 6 years or more, by conventional
cross breeding. We have designed a rapid reliable method for the identification of self-compatible varieties of ‘Osa-Nijisseiki’
offspring based on the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) with S-allele specific
restriction endonucleases. By using this method, 8 self-compatible varieties were selected among 16 selections resulting from
a cross between the self-compatible cultivar ‘Osa-Nijisseiki’ (S2Ssm
4) and the self-incompatible cultivars ‘Niitaka’ (S3S9), ‘Whasan’ (S3S5), ‘Chuwhangbae’ (S4S6). The S-genotypes of 16 ‘Osa-Nijisseiki’ offsprings were also determined.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
17.
T. Miedaner F. Wilde V. Korzun E. Ebmeyer M. Schmolke L. Hartl C. C. Schön 《Euphytica》2009,166(2):219-227
Fusarium head blight (FHB) infects all cereals including maize and is considered a major wheat disease, causing yield losses and mycotoxin
contamination. This study aimed to compare the realized selection gain from marker and phenotypic selection in European winter
wheat. A double cross (DC) combined three FHB resistance donor-QTL alleles (Qfhs.lfl-6AL and Qfhs.lfl-7BS from ‘Dream’, and one QTL on chromosome 2BL from ‘G16-92’) with two high yielding, susceptible winter wheats, ‘Brando’ and
‘LP235.1’. The base population of 600 DC derived F1 lines was on one hand selected for the respective QTLs by SSR markers (marker-selected cycle, CM), resulting in 35 progeny
possessing different combinations of beneficial donor-QTL alleles. On the other hand it was selected phenotypically, only
by FHB rating, and the best 20 lines were recombined and selfed (phenotypically selected cycle, CP). The variants CP, CM,
and an unselected variant (C0) were tested at four locations by inoculation of Fusarium culmorum. Resistance was measured as the mean of multiple FHB ratings (0–100%). FHB severity was reduced through both phenotypic and
marker selection by 6.2 vs. 5.0%, respectively. On a per-year basis, marker selection by 2.5% was slightly superior to phenotypic
selection with 2.1%, because the first variant saved 1 year. Marker-selected lines were on average 8.6 cm taller than phenotypically
selected lines. A high genetic variation within the marker-selected variant for FHB resistance and the high effect of a resistance-QTL
allele on straw length indicate that additional phenotypic selection will further enhance selection gain. 相似文献
18.
In vitro shoots of six pear (Pyrus communis L.) cultivars, ‘Conference’, ‘Doyennéd'Hiver’, ‘Passe Crassane’, ‘Bartlett’, ‘AbbéFetel’ and ‘Butirra Precoce Morettini’
were irradiated with gamma rays (3.5 Gy). After three subcultures, microcuttings from the irradiated shoots and from additional
non-irradiated microcuttings were rooted to establish plants for survey orchards. All trees were individually observed for
variation in fruit traits and for productivity. Trees were selected for improved characters related to production such as
early bearing and consistent productivity. Variations observed in fruit appearance concerned degree of russeting, fruit shape
and size. The frequencies of the observed variations in fruit traits depended on the cultivar, ranging from 0.81% in ‘Doyennéd'Hiver’
to 3.64% in ‘Passe Crassane’. Of the 97 variants selected, only two showed chimeral behavior.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
19.
Interspecific hybridization was carried out between Lilium longiflorum and L. lophophorum var. linearifolium by using the cut style method of pollination, as a contrast, intraspecific hybridization between L. longiflorum ‘Gelria’ and L. longiflorum was also made, but no mature seeds and offspring were obtained from the two combinations under in vivo condition. Ovules
excised from each carpel 5–35 days after pollination (DAP) were cultured on B5 or half-strength B5 medium containing sucrose
at different concentrations in vitro. In L. longiflorum × L. lophophorum var. linearifolium, only 1.17% of ovules excised at 10 DAP developed into seedlings, and in L. longiflorum ‘Gelria’ × L. longiflorum, only 0.99% of ovules excised at 25 DAP developed into seedlings; none of the ovules excised at other different DAP in the
two cross combinations produced any seedlings. The results showed that interspecific hybridization had a more serious post-fertilization
barrier than the intraspecific hybridization, and that a lower concentration (3%) of sucrose led to better embryo development
and higher percentage of seedlings in ovule cultures. All hybrid seedlings obtained were successfully transplanted to soil
and grew normally. The progenies investigated were identified as true hybrids based on inter-simple sequence repeat (ISSR)
analysis. 相似文献
20.
In vitro selection of strawberry (Fragaria × ananassa Duch.) clones tolerant to salt stress 总被引:1,自引:0,他引:1
The method of in vitro selection for increased salt tolerance at the seed germination and early growth phase of strawberry seedlings is proposed.
Clone Pau/27 was selected on medium containing 200 mM of NaCl from population obtained by free pollination of cv. ‘Paula2019;.
Subsequently, on the same medium 18 salt tolerant clones were attained from the population of seeds collected from self pollinated
Pau/27 plants. In the next step we examined the influence of a mild salt stress (75 mM of NaCl) on vegetative growth parameters
of selected clones and two cvs.; ‘Paula2019; and ‘Senga Sengana’. All materials in the study on the basis of calculated indexes
were divided into four groups differing in reaction to salt. First group (clustered together cv. ‘Paula’, Pau/27 and three
second generation clones: Pau/27/11, Pau/27/24, Pau/27/30) represents sensitive genotypes. Second group, including ‘Senga
Sengana’, Pau/27/06, Pau/27/10, Pau/27/12, Pau/27/13, Pau/27/15, Pau/27/18, Pau/27/20, Pau/27/21, Pau/27/26, Pau/27/27, Pau/27/31
and Pau/27/32 was designated tolerant. Third group contains only one highly tolerant clone Pau/27/08. The last group comprises
two highly sensitive clones (Pau/27/01 and Pau/27/03).
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献