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1.
The aim of this study was to determine signs of subclinical endometritis found by ultrasound that are associated with reduced fertility in dairy cows.The maximum diameter of the uterine lumen was determined by ultrasound in 324 cows without clinical signs of endometritis after evaluation of the genital tract 21 to 27 days postpartum. Cows were classified into healthy or with subclinical endometritis by three threshold values for the maximum uterine lumen diameter of 0.2 cm, 0.5 cm or 0.8 cm. Examinations by rectal palpation and ultrasound as well as classifications were repeated 14 days later. In addition, ovaries were scanned by ultrasound to determine the stage of the estrous cycle. In a subgroup of 103 cows the echotexture of the uterus and its contents was evaluated. In these cows the diagnosis of subclinical endometritis was performed by a scoring system. The diameter of the uterine lumen was significantly affected by stage of the estrous cycle at the time of examination. However, no effects were found for the stage of the cycle at the time of examination on subsequent reproductive performance. A uterine lumen with a maximum diameter of more than 0.2 cm showed a significant negative association with conception rate and proportion of cows pregnant. Classification based on higher threshold values did not reveal an association with reproductive performance. Echogenic content in the uterus also decreased reproductive performance. A classification based on the echotexture of the uterus and its contents revealed significant differences between healthy cows and cows with subclinical endometritis regarding the proportion of cows inseminated and pregnant.The results of this study showed that the diagnostic of bovine endometritis should be broadend by ultrasonography. The definition of subclinical endometritis diagnosed by means of ultrasonography has to be evaluated in further studies. 相似文献
2.
In dairy cattle, uterine infections are not life threatening and often unavoidable; however, they reduce fertility and increase the production costs of properties. The aim of this study was to evaluate the incidence of subclinical endometritis from 32 to 70 days in milk (DIM) and its effects on the reproductive performance of crossbred dairy cows. Lactating cows (Holstein/Gir; n?=?172), with no history of retained placenta, without clinical signs of uterine infection were used. The body condition score (BCS) was evaluated on a scale from 1 to 5. Ultrasound examination was performed to evaluate uterine lining and ovarian activity, while vaginal mucus was analyzed by gloved hand. The diagnosis of subclinical endometritis was performed by endometrial cytobrush technique. The samples were collected, stained, and examined microscopically; positive cases for subclinical endometritis were considered with the presence of ≥5 % of neutrophils. Later, the cows were submitted to conventional artificial insemination or timed artificial insemination. The incidence of subclinical endometritis in the herd was 26 %, and this was not affected by the season of calving, presence of corpus luteum, DIM, and parity. Cows with a BCS ≤2.50 had a higher incidence of subclinical endometritis. The conception rate to first insemination and pregnancy rate at 150 days postpartum were not influenced by the presence of subclinical endometritis in crossbred dairy cows. 相似文献
3.
A certain level of endometrial bacterial infection and inflammation is involved in bovine uterine involution during the puerperal period. Factors that hamper normal uterine involution expose the uterine environment to pathological conditions, causing different endometritis levels. The lack of proper diagnostic tools extends the time to conception. Efforts have been made to elucidate the postpartum uterine environment, including bacterial flora, changes in transient endometrial inflammation, and the pathophysiology of endometritis, to improve bovine reproductive performance. E. coli and Trueperella pyogenes in the uterus are likely to cause persistent infection, and Mycoplasma bovigenitalium infection is associated with dystocia and cytological endometritis in postpartum dairy cows. Due to the widespread use of cytobrush as a diagnostic tool for bovine subclinical endometritis (SE) that enables quantification of the degree of inflammation, we found that endometritis at week 5 postpartum was associated with delayed first ovulation. Approximately 30% of open cows have SE during the postpartum period, and cows with low blood glucose during prepartum have a high risk of developing SE. Additionally, cows with purulent vaginal discharge do not always have endometritis but only vaginitis and/or cervicitis. Intrauterine infusion of polyvinylpyrrolidone-iodine (PVP-I) improves fertility and promotes endometrial epithelial cell regeneration after inducing transient uterine inflammation, suggesting that PVP-I could be a good alternative to antibiotics. In conclusion, prepartum management to prevent glucose deficiency, prompt diagnosis to identify causative agents and intrauterine inflammation levels, and appropriate treatment to minimize antimicrobial resistance is beneficial for tackling endometritis and improving reproductive performance in bovine herds. 相似文献
4.
子宫内膜炎是奶牛产后普遍发生的一种生殖疾病,可导致奶牛产犊间期延长、产奶量降低、淘汰率增加、治疗期间奶源废弃、管理及治疗成本增加,也是导致奶牛不孕症的重要原因之一。目前国内外防治奶牛子宫内膜炎的诊断技术和治疗方法众多,主要有阴道镜检查、Metricheck装置评估、子宫细胞学检查、抗生素疗法、中药疗法、中西医结合疗法、激素疗法、臭氧新型疗法等诊疗技术。阴道镜检查是一种可视化检查方法,利用内窥镜直观评估奶牛阴道内分泌物性状确定子宫内膜炎严重程度;Metricheck装置是根据阴道内分泌物含量和性状,设置不同评分值评估产后奶牛子宫内膜炎和繁殖性能;子宫细胞学检查通过子宫细胞刷、灌洗或活检技术确定子宫内膜细胞和中性粒细胞(PMN)的比例,设定奶牛产后不同时期PMN最佳阈值诊断子宫内膜炎;抗生素疗法通过宫内灌注、皮下注射、静脉注射抗生素等途径治疗奶牛子宫内膜炎,治疗成本低,见效快,是目前治疗奶牛临床子宫内膜炎最常用的疗法;中药疗法筛选不同组分的中药复方制剂,通过调节奶牛机体免疫系统,改善子宫内血液循环,抑制致病菌繁殖,促进子宫恢复正常生理机能;中西医结合疗法兼具中药辩证治疗的优点和西药见效快的特点,临床治疗奶牛子宫内膜炎具有广泛应用价值;激素疗法中前列腺素通过诱导黄体溶解,消除黄体酮产生和免疫抑制,刺激子宫复旧,降低产后奶牛长期子宫感染和炎症的风险;臭氧新型疗法是将臭氧制作成泡沫制剂、油制剂等产品通过宫内灌注杀灭病原菌。这些诊疗技术在治疗奶牛子宫内膜炎的过程中均取得了不同程度的效果,对评估产后奶牛繁殖性能和生产性能具有良好的指导作用。文章通过介绍这些诊疗技术在奶牛子宫内膜炎中应用的研究进展,以期为该病的防治提供参考。 相似文献
5.
A study was carried out on 59 clinically healthy Zebu x Friesian crossbred cows to determine the prevalence of subclinical
endometritis, associated factors and its effect on the reproductive performance. Subclinical endometritis was diagnosed by
endometrial cytology using uterine lavege technique and subclinical mastitis using CMT. The clinical, management and reproductive
data were obtained from a weekly follow up visit of each cow for a period of 6 months after calving. The prevalence of subclinical
endometritis, with ≥5% neutrophil count, was 47.5% and 30.5% at week 4 and 8, significantly decreasing (P = 0.002) as postpartum
period advanced. Body condition score at week 4 postpartum (OR = 4.5, P = 0.017) and regular cow exercise (OR = 4.8, P = 0.026)
were the significant risk factors while post-calving hygiene (P = 0.06) was poorly associated. Subclinical endometritis was
also directly associated with subclinical mastitis at both week 4 (OR = 4.5, P = 0.012) and 8 (OR = 3.6, P = 0.031) postpartum.
The risk of first service pregnancy (OR = 5.1, P = 0.004) was higher in cows negative for subclinical endometritis at week
8 postpartum and the proportion of cows that required more than 3 services was higher in cows with uterine inflammation. Also
the proportion of cows diagnosed pregnant within 180 DIM was higher in cows with normal uterus at both week 4 (OR = 10.3,
P = 0.001) and week 8(OR = 21.8, P = 0.001). These results indicated that subclinical endometritis was directly associated
with poor body condition and subclinical mastitis and that it had negative effect on reproductive traits of dairy cows. This
association may also reflect the possibility of translocation of bacteria/bacterial products from the uterus to the udder
or vic-versa or else the presence of common cause for both endometritis and mastitis. 相似文献
6.
Endometritis is an inflammation of the endometrial lining of the uterus without systemic signs, which is associated with chronic
postpartum infection of the uterus with pathogenic bacteria. Nitric oxide (NO) is an inflammatory mediator that among other
effects causes smooth muscle relaxation and mediated cytoimmunity and inflammation toxicity. To see if the nitric oxide concentration
in plasma and uterine secrets is related with postpartum endometritis, NO concentrations in plasma and uterine secrets were
measured in dairy cows with puerperal endometritis (clinical endometritis ( n = 60) and subclinical endometritis ( n = 58)). Cows with clinical or subclinical endometritis showed higher concentrations of NO in both plasma and uterine secrets
when compared with normal cows and the highest concentrations of NO in plasma and uterine secrets were found in dairy cows
with clinical endometritis. Expression level of NOS2 mRNA in endometrial biopsies from cows with puerperal endometritis was
also higher and the highest expression of NOS2 mRNA was found in cows with clinical endometritis. The results showed that
concentrations of NO in plasma and uterine fluid are related with the degree of endometritis which may be useful to diagnose
the endometritis in dairy cows. 相似文献
7.
OBJECTIVE: To determine the effect of intrauterine cephapirin (0.5 g) treatment on the reproductive performance of cows diagnosed as at risk of developing endometritis. METHODS: Cows (n = 1325) from 17 seasonal calving dairy herds were enrolled if they had been calved for more than 6 days and had a condition that placed them at risk of endometritis. Half were treated with intrauterine cephapirin at an examination performed 28-37 days prior to mating start date (MSD). Cows were scored for body condition, their vaginal discharge was scored using vaginoscopy (VV), and the uterus and ovaries were assessed by rectal palpation. RESULTS: Overall, there was no significant treatment effect on reproductive performance. In 945 modified at risk (MAR) cows the effect of treatment varied with the calving to treatment interval (CTI) and VV status. For the subset of 229 MAR cows with CTI < or = 28 days, treatment improved the proportions of first-service conception (OR 3.1; P < 0.01) and 6-week in-calf (OR 2.1; P < 0.05), and reduced the mean MSD to conception interval by 20 days (P < 0.05). The difference was greater in cows with a positive VV score (1-3). Treated VV-negative cows with CTI > 48 days had a reduced proportion of cows in-calf by 6 weeks (OR 0.58; P = 0.056) compared with untreated cows. CONCLUSION: Intrauterine cephapirin infusion of MAR cows improved reproductive performance, depending on CTI. The greater treatment effect in VV-positive cows suggests that VV scoring is a useful tool for the diagnosis of endometritis. 相似文献
8.
Two methods for a scheduled post partum examination were compared on a commercial dairy farm. In Group 1, all cows (n = 601) were examined by rectal palpation between day 20 and 26 post partum for signs of endometritis (vaginal discharge, enlarged uterus). In Group 2, all cows (n = 652) were examined by external inspection for vaginal discharge as a sign for endometritis. In both groups all cows with endometritis were treated twice with prostaglandin F2 alpha (25 mg of dinoprost) in a 14-day interval. The proportion of cows with signs of endometritis was 33.3% and 17.2% in Group 1 and Group 2, respectively (p < 0.05). In Group 1, the conception rate (39.0% vs 49.3%) and the proportion of cows pregnant (60.5% vs 72.6%) were lower for cows with endometritis than for cows without endometritis. In Group 2, no significant differences were found in reproductive performance for cows with and without signs of endometritis. No significant differences in reproductive performance were found for cows with endometritis between the two groups. Also, for cows without endometritis no significant differences in reproductive performance were found between the groups. It is concluded that the post partum examination by rectal palpation was more sensitive in finding cows with endometritis. However, with regard to the reproductive performance the more sensitive method was not more effective than the method based on systematic external inspection. 相似文献
9.
The objectives of the study were to characterize an ultrasound reproductive tract scoring (URTS) system to assess suitability for breeding in dairy cows, to describe the prevalence of these scores in commercial dairy herds and to examine their relationship with subsequent fertility. Ultrasound examinations (7797) were performed on 5751 Holstein–Friesian cows prior to breeding in 62 seasonally calving herds over 2 years. Data recorded from images of both ovaries and the uterus were combined into a six point scoring system and the prevalence of cows with cystic ovarian follicles and uterine abscesses and adhesions was recorded separately. The prevalence of ovulatory cows which had completed uterine involution (score 1), or had mild (2) or moderate endometritis (3) was 62.5%, 21.7% and 2.2%, respectively. The prevalence of anovulatory cows with moderate endometritis (4), ovulatory cows with pyometra (5) and anovulatory cows which had completed uterine involution (6) was 3.3%, 2.2% and 8.1%, respectively. The interval between calving and examination differed between cows with each of the scores 1, 2, 5 and 6 (61, 46, 53 and 50 days, respectively, p < 0.05) but not between cows with scores 3 and 4 (37 and 35 days, respectively). Ovulatory cows which had completed uterine involution (score 1) had a higher likelihood of pregnancy to first service than ovulatory or anovulatory cows which had not completed uterine involution (p ≤ 0.10). The prevalence of cows with cystic ovarian follicles or abscesses or adhesions of the reproductive tract was 3.9% and 1.2%, respectively. In conclusion, 29% and 11% of cows in seasonally calving and breeding dairy herds had not completed uterine involution or were anovulatory prior to the mating start date, respectively. Both conditions, detected using a URTS system, significantly reduced first service pregnancy rate in these pasture-based dairy herds. 相似文献
10.
Endometritis is an inflammation of the uterine lining that is commonly initiated at parturition. The degree of its effect on fertility varies with the severity of the inflammation, the time required for resolution of endometrial lesions, and the extent of permanent changes that impair endometrial gland functions and/or alter the uterine and/or oviductal environment. The primary nonspecific organisms associated with endometrial pathology are Corynebacterium pyogenes and the gram-negative anaerobes. The majority of postpartum dairy cows have some degree of endometritis but resolve it by 40 to 50 days post partum. Cows with certain periparturient disorders involving the reproductive tract and/or a defective host immune mechanism may acquire persistent infections that impair subsequent fertility. Not all infertile cows or all cows with positive uterine cultures have endometritis. Cows recovering from C. pyogenes endometritis may require 1 month after clearance of the organism for fertility to be restored. These cows, as well as many cows with slightly delayed uterine involution at 30 days post partum, do not benefit from antimicrobial therapy. The effect of endometritis varies between herds. It is associated with prolongation of calving intervals that can vary from less than 2 weeks to 2 months or more. The cost of days open beyond 80 to 110 days post partum is currently estimated to be $2.00 to $2.25 per day. Evaluation of the bovine genital tract is best conducted by a rectal examination combined with a vaginal speculum examination. Cows selected for therapy for endometritis should meet the strict requirements of a grossly enlarged uterus and a severely abnormal uterine discharge. Induction of estrus is the treatment of choice whenever possible. When antimicrobial therapy is indicated, tetracycline is recommended for intrauterine use during the early postpartum period when mixed bacterial populations are present. Commonly used doses are 2 to 3 gm. For systemic signs of illness, systemic administration of penicillin has been recommended. Twice daily doses of 5000 to 10,000 IU of penicillin per kg may be necessary to maintain therapeutic blood concentrations. Systemic administration of an antimicrobial is necessary to achieve therapeutic concentrations in the oviducts, cervix, and vagina. With chronic endometritis due to C. pyogenes, intrauterine administration of 1 to 1.5 X 10(6) IU of procaine penicillin G has been recommended. Multiple daily treatments are desirable. Milk from treated cows should be tested for penicillin before it is added to bulk tank milk. It still is not clear whether antimicrobial therapy is cost effective.(ABSTRACT TRUNCATED AT 400 WORDS) 相似文献
11.
Data from 3276 cows with 6598 lactations in 85 herds participating in a herd health monitoring programme were selected for an observational study. Cases of endometritis were diagnosed based on a clinical examination after 21 days postpartum. The cases were grouped by the severity of endometritis (mild, severe) and by the treatment performed (intrauterine infusion, prostaglandin injection, no treatment). The conception rates and the calving-to-conception intervals of these groups were compared statistically. Conception rate was lower in cows with severe endometritis compared to cows with mild endometritis while it did not differ between treatment groups. In cows with mild endometritis a significantly longer calving-to-conception interval was observed if intrauterine infusions were performed compared to the other two treatment options. The occurrence of other fertility problems during the same service period had a significant effect on conception rate and calving-to-conception intervals. In cows with severe endometritis intrauterine or prostaglandin treatments appeared to be beneficial, whereas intrauterine infusions in cows with slight endometritis had a negative effect on reproductive efficiency. 相似文献
12.
The objective of this study was to evaluate the prevalence of clinical endometritis and its impact on reproductive performance in grazing dairy cattle in Argentina to compare data with previous reports from herds kept in confinement housing systems. A total of 243 Holstein dairy cows from three commercial dairy farms in Buenos Aires Province (Argentina) were examined for the signs of clinical endometritis 18-38 days postpartum (dpp) by external inspection and manual vaginal examination. Vaginal discharge was scored into the categories VDS 0 (transparent, clear mucus), VDS 1 (mucupurulent discharge), VDS 2 (purulent discharge) and VDS 3 (purulent discharge with fetid odour). Cows diagnosed with VDS 1 to VDS 3 were regarded as affected with clinical endometritis and cows with VDS 0 as free of clinical endometritis. All cows were re-examined 14 days later following the same examination protocol. Prevalence of clinical endometritis 18-38 dpp was 35% and decreased to 18% at re-examination. Cows with no palpable ovarian structures or periparturient disorders were at higher risk for clinical endometritis. Hazard for pregnancy was significantly lower in cows with purulent or fetid odour discharge compared with reference cows with no discharge (HR=0.49; p=0.01), resulting in a lower proportion of cows pregnant by 360 dpp (66% vs 78%). Furthermore, the number of services per pregnancy was higher for cows with clinical endometritis than for cows without clinical endometritis (4.4 vs 3.1; p=0.04). Cows with clinical endometritis were 1.6 times as likely to be culled as cows with no signs of clinical endometritis. In conclusion, the prevalence and the impact of clinical endometritis in a pasture-based, extensive dairy production system in Argentina were similar to previously published data from dairy farms with confinement production systems. 相似文献
13.
Endometritis is a postpartum uterine disease of cows that interrupts reproductive cycles resulting in suboptimal fertility, reduced performance and profitability of the dairy herd. The objective of the study was to estimate the perceived and observed prevalence of endometritis among zero-grazed dairy cows in smallholder farms in Rwanda. A snowball sampling method was applied in cross-sectional survey to obtain data from 370 farms on 466 cows within their 21–60 days postpartum (dpp). The survey, conducted from September 2018 to March 2019, simultaneously examined cows using the Metricheck Device (MED) to determine the presence and type of vaginal mucus (VMC) based on a score scale of 0 to 3. Cows scoring VMC ≥ 1 were recorded as clinical endometritis (CLE)–positive. Cytotape (CYT) was used to determine the percentages of polymorphonuclear cells (PMN) in endometrial cytology sample. Cows with ≥ 5% PMN were recorded subclinical endometritis (SCLE)–positive, whereas cows with VMC-0 and < 5% PMN were considered healthy cows. At cow-level, endometritis prevalence was 70.2% with 67.2% CLE and 31.8% SCLE while at the herd-level, prevalence was 71.1% with 68.1% CLE and 34.4% SCLE. The differences between the diagnostic performance of the MED and CYT were significant (p < 0.001). Perceived prevalence by farmers was much lower (3.2%) and without agreement with the observed prevalence (kappa = − 0.02, p > 0.05). The highly observed prevalence and farmer underestimation of endometritis prevalence indicate knowledge gaps about endometritis. The extension service therefore needs to increase awareness and education among smallholder farmers about detection and management of endometritis. 相似文献
14.
Subclinical endometritis (SEM) is poorly investigated in beef cows, as stated in the literature. This project aims to evaluate the rate and the consequences of SEM in Piedmontese cows, with a focus on bacteriological findings and fertility parameters. Uterine cytology was performed for 97 subjects; a total of 31% of the cows were diagnosed as being positive for SEM and as having an 8% neutrophil (PMN) presence on the slide, which is considered as the best cut‐off to diagnose the pathology. Only 13% of the cows positive for SEM were pregnant within 130 dpp and generally showed increases of 40 days in the partum to conception interval compared with the negative cows (142 vs 182, p = 0.01). Cows positive for both bacteriology and cytology showed a lower fertility than cows with only inflammation or only a bacterial presence (p = 0.0004). Bacterial isolation detected different species, but no difference in regard to the impact of these bacteria on SEM was shown. Parity, presence of calves, hygiene condition, age and number of service did not affect whether a cow was positive for subclinical endometritis (p < 0.05). The housing system (free stalls vs tie stalls) used seems to affect the SEM rate in Piedmontese cows; cows bred in tie stalls were more likely to be positive for SEM (OR = 2.2; p = 0.04). In conclusion, cytology seems to be a good technique for the diagnosis of subclinical endometritis in beef cows, and as in dairy cows, subclinical endometritis has a detrimental effect on fertility, causing an increase in partum to conception and a decrease in the rate of cows who become pregnant within 130 dpp, particularly for those cows housed in a tie stall. 相似文献
15.
OBJECTIVE: To assess the association between the findings from vaginoscopic examination of postpartum dairy cows with peripartum reproductive disease and their subsequent reproductive performance, and to determine the factors that might be associated with a positive vaginoscopic examination (VV). METHODS: Cows (n=1325) from 17 seasonal calving dairy herds were enrolled if they had been calved at least 7 days and had at least one of the following conditions that placed them at risk of endometritis: retained fetal membranes (RFM), dystocia, a dead calf, hypocalcaemia, twin birth, calving induction or an observed vulval discharge (VD). Examination was undertaken 28-37 days before mating start date (MSD) within each herd. All cows were body condition scored (BCS) and were VV scored on a scale of 0 (clear) to 3 (purulent). The uterus and ovaries were assessed by rectal palpation. RESULTS: Cows with a positive VV score (1-3) were less likely to conceive to first service and had a lower pregnancy proportion than cows that had a negative VV score (score 0). Mean MSD to conception interval was longer in VV-positive cows. A positive VV score was associated with a low BCS, primiparity, intrapelvic uterus, poor uterine tone, large uterus, RFM, VD, dystocia, dead calf and twins. CONCLUSION: In the present study a positive VV score was associated with reduced reproductive performance and was more common in primiparous and low BCS cows. Of the at-risk cows, those with RFM, VD, dystocia, dead calf or twins were more likely to have a positive VV score. 相似文献
16.
试验旨在探究围产期亚临床酮病与泌乳早期奶牛繁殖性能、卵泡发育之间的关系,并检测试验牛血液生化指标的变化。试验在黑龙江某大型集约化牛场开展,根据产后血酮水平确定亚临床酮病组(SCK)和健康组(C)奶牛共60头,根据试验牛产后50 d内发情状况,将SCK组再分为发情组(SCKE,16头)和乏情组(SCKA,14头),C组也同样分为发情组(CE,25头)和乏情组(CA,5头)。所有试验牛在产后50 d通过直肠检查和B超检查了解子宫复旧及卵泡发育状况,记录繁殖性能数据,并进行血液生化指标分析。结果表明:与健康组发情奶牛相比,亚临床酮病发情奶牛产后首次发情日期推迟约10 d( P<0.05);产后50 d卵泡直径差异极显著(差值约4 mm)( P<0.01)。亚临床酮病乏情奶牛子宫复旧延迟发生率显著高于健康组发情奶牛( P<0.05);亚临床酮病奶牛血浆中胰岛素样生长因子-1(IGF-1)含量显著低于健康奶牛( P<0.05),而泌乳量极显著提高( P<0.01)。与发情奶牛相比,乏情奶牛血浆中甘油三酯(TG)含量显著升高( P<0.05);葡萄糖(Glu)、胰岛素(Ins)、雌二醇(E 2)、孕酮(P 4)含量显著或极显著降低( P<0.05; P<0.01)。综合以上试验结果,奶牛患亚临床酮病而导致能量代谢指标异常是引起奶牛乏情、产后卵泡发育受阻和繁殖障碍的主要因素,进而导致奶牛繁殖力下降。 相似文献
17.
There is evidence that supplementing methionine has positive effects on uterine environment, oocyte quality and embryo development in cattle. Thus, the objective of this study was to evaluate reproductive traits of cows supplemented with rumen‐protected methionine (RPM) during early to mid‐lactation in comparison with an untreated control group (CON). An additional focus was on the effect of puerperal diseases on reproductive performance parameters in RPM‐supplemented group MET and in CON. A total of 1,709 multiparous Holstein‐Friesian cows were enrolled in this field trial conducted on a commercial dairy farm in Slovakia. Cows were allocated at approximately 12 days post‐partum (dpp) to either CON or MET, the latter supplemented with 25.0 g–27.2 g RPM per cow per day incorporated into the total mixed ration (TMR) until leaving the study pen at approximately 140 dpp. The amount of RPM was calculated based on individual feed ingredients analysis and adjusted during the study period when TMR changed. Cows were monitored during the post‐partum period by vaginal examination (day 5 pp), measuring of beta‐hydroxybutyrate in blood (3, 5, and 8 dpp) and by vaginal examination, uterine cytology and measuring of back fat thickness by ultrasound (all at 31 ± 3 dpp). Compared with CON, cows supplemented with RPM did not show better reproduction performance parameters (first service submission rate, days to first service, conception risk, days open 140). Results from binary logistic regression model for the risk of conception showed that metritis had a significant effect, but the supplementation of methionine had not. Results of Cox regression analysis for the odds of conception within 140 dpp revealed only metritis and clinical endometritis as significant factors. In conclusion, supplementation of RPM had no beneficial effect on reproductive performance in this study farm compared with an untreated control group. 相似文献
18.
The objectives of this study were to investigate the associations between peripheral blood neutrophil (PMN) function, energy status, and uterine health in periparturient dairy cows. Data were collected from 83 multiparous Holstein cows. Blood samples for PMN function determination were collected weekly from 1 week prior to calving (week -1) through 4 weeks after calving and again at 8 weeks after calving. Energy metabolites were measured and dry matter intake (DMI) was determined from weeks -2 to 5 to evaluate energy status of cows during the periparturient period. All cows were examined for uterine health disorders. Blood PMN killing ability was evaluated by determining myeloperoxidase activity and cytochrome c reduction activity in isolated blood PMN's. For cows that were diagnosed with puerperal metritis and subclinical (SC) endometritis and puerperal metritis, blood PMN functions were significantly (P<0.05) impaired during the periparturient period, compared to cows with normal uterine health. Cows with subclinical endometritis and puerperal metritis or SC endometritis also had significantly (P<0.01) higher NEFA and significantly (P<0.001) lower DMI during the periparturient period, and significantly (P<0.05) higher BHBA during early lactation, compared to cows with normal uterine health. Neutrophil function was also significantly (P<0.01) impaired in cows with peripartum negative energy balance, which was characterized by elevated blood levels of NEFA and decreased DMI. Decreased PMN function and energy balance were associated with uterine health disorders and the decreases in PMN function and energy balance occurred prior to parturition and prior to the detection of these uterine disorders. 相似文献
19.
非特异性子宫感染会降低奶牛的生产性能和奶牛场的潜在效益。发生子宫感染时常并发感染化脓性放线杆菌或混合感染其他细菌。当从产后21 d的奶牛子宫液中分离到化脓性放线杆菌时,说明奶牛发生了严重的子宫内膜炎,并且将导致产后第一个发情期不孕。然而引起子宫感染的确切原因尚不清楚,但可以肯定与多种因素有关。发生难产、胎衣不下、怀有双胎或死胎以及患有各种代谢紊乱疾病的奶牛比其他奶牛更容易发生子宫感染。分娩前后异常的免疫功能更容易导致发生严重的子宫感染。子宫感染一般不会引起奶牛死亡,但是发生子宫感染的奶牛其生产性能会受到很大影响。另外,子宫发生感染还会降低产奶量,并且对子宫感染的一些治疗措施还会对牛奶产生污染。因为感染的非特异性,所以子宫感染很难预防;注意加强卫生设施以及围产期的卫生,特别是在助产过程中的卫生,才可能是最好的预防方法。异常免疫功能导致奶牛发生子宫感染的现象,说明通过对免疫功能的调节来预防和治疗围产期奶牛子宫感染是有潜力的。 相似文献
20.
Background: It is known that quantification of bovine acute‐phase proteins could routinely provide early diagnostic and prognostic information for monitoring herd health status. Objective: The aim of this study was to test the hypothesis that haptoglobin (Hp) and serum amyloid A (SAA) have the potential for indicating uterine infection, mainly due to subclinical endometritis, which can seriously influence the reproductive performance in dairy cows. Methods: A total of 264 serum samples were collected from 24 Holstein dairy cows at defined intervals from 1 week prepartum to 6 months postpartum. Clinically healthy animals (Group 1, n=6) were compared with 24 animals having acute puerperal metritis (Group 2, n=18) and with concentrations obtained from healthy heifers (baseline values, n=10). The concentrations of serum Hp and SAA were measured using a hemoglobin‐binding assay and Western blot analysis, respectively. Reproductive performance was assessed as the number of days open and the conception rate at the end of the study. Results: Upper cut‐off values for Hp and SAA concentrations in heifers were 130.9 μg/L and 51.9 μg/mL, respectively. Hp concentrations in Group 2 were significantly higher than those in Group 1 at all time points ( P<.001). Additionally, among the successfully pregnant animals, the number of days open was significantly higher in 6 cows with Hp >130.9 μg/mL (median=316 days) than in 11 cows with Hp ≤130.9 μg/mL (median=120 days) ( P=.0024). Conclusions: Serum Hp and SAA concentrations can be used to recognize uterine infection in postpartum cows. The association between elevated Hp values and number of days open suggests Hp may also be a useful indicator of poor prognosis for reproductive performance. 相似文献
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