Fingerprinting Analysis of the Introgressed lines from Gossypium hirsutum L.× G.barbadense L.based on AFLP markers

The main cultivated varieties in the world belong to the species of upland cotton (Gossypium hirsutum L.),and their genetic background is very narrow.However,the wild species and races in the genus of Gossypium have abundant genetic diversity and possess lots of excellent genes with potential high yield,fine and strong fiber,disease and insect resistance,drought and coldness resistance,male sterility,and so on.     

Construction of Molecular Linkage Map of Cultivated Allotetraploid Cotton (Gossypium hirsutum L.×G. barbadense L. )with SSR and RAPD Markers

A permanent doubled haploid population fromthe crossing of G.hirsutum × G.barbadensewere developed by means of Vsg,virescentlymarked semigamy line in sea island cotton,which was characterized by a cytologicalmechanism for developing haploids with certainconvenience,and thus constructed an     

Mapping and Tagging the QTLs of Yield Traits (Gossypium hirsutum L. )with SSRs and RAPDs in Upland Cotton

Molecular markers provide the opportunity toidentify marker-quantitative trait locus(QTL)associations in different environments andpopulations,and can be used to improve theefficiency of conventional plant breeding bycarrying out indirect selection through molecularmarkers linked to the interest traits of QTL at     

Heredity, Reproductive and Adaptive Heterosis and Variability of Intraspecies Hybrids of G. hirsutum L.

On the patterns of geographically remote G.hirsutum L.cotton hybrids produced byintraspecies hybridization of local and importedbreeds we have investigated particularities inheredity and variability of the most importanteconomical and physiological features,display ofreproductive and adaptive heterosis in different     

陆地棉(G.hirsutum L.)种子活力的研究

本文通过棉花种子活力与电导率、阳离子外渗、组织结构、凝集素和种子加工的关系研究表明,种子活力下降,阳离子外渗增加,其中主要是K~+。种子各部分对逆境条件的反应不同,越是活跃的组织越易丧失活力。高活力的种子也是高凝集素的种子,在多逆抗性的选择过程中,利用低温发芽是对种子高活力和高凝集素等抗性物质的选择。因此,可考虑用电导率、胚根和下胚轴的比值作为检验棉子活力的指标。并证明棉子经我国自行研制的泡沫酸脱绒机处理,能有效地提高播种品质。     

Phenotype Variations in Plant Regeneration of Upland Cotton (Gossypium hirsutum L.)

以陆地棉冀无2031无菌苗下胚轴为外植体，在MS中附加激素0.1mg/L IAA+0.1mg/L 2,4-D+0.1mg/L KT的培养基上诱导出愈伤组织。选择疏松愈伤组织继代到附加0.1mg/L ZT的培养基中，经2~3个月的继代培养诱导出颗粒状疏松胚性愈伤组织。胚性愈伤组织在无激素、每升加2g谷氨酰胺和0.5g天门冬酰胺的胚性愈伤增殖萌发培养基上培养2个月左右，可陆续形成不同时期的体胚苗。对冀无2031不同时期的体细胞萌发后的体胚苗进行观察，发现了真叶畸形苗、子叶节生根胚、胚轴侧生根胚、腋芽丛生苗和以腋     

Genetic Engineering of Cotton (Gossypium hirsutum L. ) for Insect-resistance

In order to improve insect-resistance of cottonand cultivate new cotton varieties,tissue cultureand plant regeneration of cotton(Gossypiumhirsutum L.)were studied with Xinluzao 4,Xi550,Jizi 492,Hengwu 89-30,Han 93-2 and Jizi123.A system of cotton tissue culture for rapid     

Identification of DNA Markers for Cotton Leaf Curl Disease (CLCD) in Cotton (Gossypium hirsutum L. )

Epidemic of cotton leaf curl virus disease(CLCD)was the compelling factor to devise newstrategies in cotton breeding programs ofPakistan.The evaluation of cotton genotypesagainst the CLCD resistance is difficult,expensive and time consuming in field andespecially in greenhouse due to unevendistribution of the disease.A marker-assisted     

Cloning and Sequence Analysis of a Steroid 5a Reductase Gene from Cotton (Gossypium hirsuturm L. ) Fiber

Brassinosteroids(BRs)are natural growth-promoting products found at low levels inpollen,seeds,and young vegatative tissuesthroughout the plant kingdom.Recently,thenotion that BRs are essential for plant growthand development has been widely accepted bythe discovery of BR dwarf mutants of     

Development and Use of Radiation-induced Chromosomal Translocations and Primary Monosomics of Cotton(G. hirsutum L. )

Over 200 disomie plants with translocations ofcotton were recovered as heterozygotes followingused by four types of treatments:combinedtreatment of seeds with colchicines and Y-rays,irradiation of seeds by fast and thermal neutronsand γ-irradiation of pollen.Numbers oftranslocations obtained were differed in M1, M2and M3 generation after irradiation and aftertreatment by different doses of irradiation.     

Development of SSR Markers towards Genetic Mapping in Cotton(Gossypium hirsutum L. )

Availability of informative molecular markers isa prerequisite for genetic mapping and marker-assisted selection projects.Micro-satellites orSimple Sequence Repeat(SSR)markers arePCR-based and currently the most widely usedmarker system in the plant molecular geneticscommunity due to their high degree ofpolymorphism,random distribution throughoutthe genome and their suitability for highthroughput genotyping formats.Despite itsglobal economic importance,cotton has slower     

Nitrogen use efficiency of cotton (Gossypium hirsutum L.) as influenced by wheat–cotton cropping systems

Wheat–cotton rotations largely increase crop yield and improve resources use efficiency, such as the radiation use efficiency. However, little information is available on the nitrogen (N) utilization and requirement of cotton under wheat–cotton rotations. This study was to determine the N uptake and use efficiency by evaluating the cotton (Gossypium hirsutum L.) N use and the soil N balances, which will help to improve N resource management in wheat–cotton rotations. Field experiments were conducted during 2011/2012 and 2012/2013 growing seasons in the Yangtze River region in China. Two cotton cultivars (Siza 3, mid-late maturity with 130 days growth duration; CCRI 50, early maturity with 110 days growth duration) were planted under four cropping systems including monoculture cotton (MC), wheat/intercropped cotton (W/IC), wheat/transplanted cotton (W/TC) and wheat/direct-seeded cotton (W/DC). The N uptake and use efficiency of cotton were quantified under different cropping systems. The results showed that wheat–cotton rotations decreased the cotton N uptake through reducing the N accumulation rate and shortening the duration of fast N accumulation phase as compared to the monoculture cotton. Compared with MC, the N uptake of IC, TC and DC were decreased by 12.0%, 20.5% and 23.4% for Siza 3, respectively, and 7.3%, 10.7% and 17.6% for CCRI 50, respectively. Wheat–cotton rotations had a lower N harvest index as a consequence of the weaker sink capacity in the cotton plant caused by the delayed fruiting and boll formation. Wheat–cotton rotations used N inefficiently relative to the monoculture cotton, showing consistently lower level of the N agronomic use efficiency (NAE), N apparent recovery efficiency (NRE), N physiological efficiency (NPE) and N partial factor productivity (NPFP), particularly for DC. Relative to the mid–late maturity cultivar of Siza 3, the early maturity cultivar of CCRI 50 had higher N use efficiency in wheat–cotton rotations. An analysis of the crop N balance suggested that the high N excess in preceding wheat (Triticum aestivum L.) in wheat–cotton rotations led to significantly higher N surpluses than the monoculture cotton. The N management for the cotton in wheat–cotton rotations should be improved by means of reducing the base fertilizer input and increasing the bloom application.     

Effect of Moderate Drought-Stress on Flowering Time of Interspecific Hybrid Progenies (Oryza sativa L. × Oryza glaberrima Steud.)

Journal of Crop Science and Biotechnology - We examined the effects of drought stress on flowering time, grain yield, and agronomic traits using 10 upland-adapted rice genotypes, i.e. eight...     

Genetic Diversity in Upland Cotton (Gossypium hirsutum L. ) Cultivars Based on RAPDs and SSRs

Quantification and classification of diversity ingermplasm collection is important for bothgenetic researchers and plant breeders.Someadvance was made in this area in the world(Liuet al,2000)based on SSRs and in China(Xu etal,2001;2002)based on RAPDs.In thisresearch,72 cultivars including 14 latestintroduced and 30 Bt-transformed ones were     

Quantitative trait locus mapping for Verticillium wilt resistance in a backcross inbred line population of cotton (Gossypium hirsutum × Gossypium barbadense) based on RGA-AFLP analysis

Verticillium wilt (VW), caused by Verticillium dahliae Kleb., is one of the most important diseases in cotton. The objective of this study was to map quantitative trait loci (QTLs) conferring VW resistance using resistance gene analog (RGA)-targeted amplified fragment length polymorphism (RGA-AFLP) markers in an interspecific backcross inbred line mapping population, consisting of 146 lines from a susceptible Sure-Grow 747 (Gossypium hirsutum L.) × resistant Pima S-7 (G. barbadense L.) cross. VW resistance was evaluated in replicated tests based on disease incidence in the field, and disease incidence and severity in the greenhouse. Of 160 polymorphic RGA-AFLP markers, 42 were significantly correlated with one or more VW traits and 41 were placed on a linkage map which covered 1,226 cM of the cotton genome and contained 251 other molecular markers. Three QTLs for VW resistance were detected, each of which explained 12.0–18.6 % of the phenotypic variation. Two of these QTLs for disease incidence and severity detected in the greenhouse inoculation tests using root wounding are located on chromosome c4. Both are closely linked to four RGA-AFLP markers and therefore considered as the same QTL for VW resistance. The other QTL detected in the field test was located on c19 and flanked by several RGA-AFLP markers. The desirable QTL allele on c4 for VW resistance detected in the greenhouse was from the VW susceptible Upland parent and absent from the resistant Pima parent which was more VW susceptible due to the disarmament of the first line of defense mechanism due to root wounding during inoculation. The other desirable VW resistance QTL allele, on c19, was from the resistant parent Pima S-7, consistent with the fact that Pima cotton was more resistant to VW when naturally infected in the field. The results should facilitate the development of more sequence specific markers and the transfer of VW resistance from Pima to Upland cotton through marker-assisted selection.     

Development and Application of a Transformation-competent Artificial chromosome (TAG) Genomic DNA Library in Allotetrapolid Cotton (Gossypium hirsutum L. )

The technology of cloning and transferring oflarge DNA fragments in plants is important forhigh-efficient identification of new genes andstudy of gene functions.Tranformation-competent artificial chromosome(TAC)vectorsystem has been shown to be very useful forefficient gene isolation in Arobidopsis thaliana.In order to develop an efficient platform for gene     

Genotype by Environment (G×E) Interaction Study on Yield Traits in Different Maturity Groups of Rice

Journal of Crop Science and Biotechnology - Rice production is affected by emerging problems of climate change and over-utilization of resources. To obtain consistent yield across diverse...     

Genetic Diversity and Its Correlation with Heterosis of Upland Cotton (Gossypium hirsutum L. ) Cultivars of Huang-Huai Region in China Evaluated by RAPD

Sixteen cultivars of Upland cotton(Gossypiumhirsutum L.)cultivars in Huang-Huai Cotton-growing Region were detected by RAPD whilethe F_1 heterosis of each hybrid involved thesecultivars were evaluated.The genetic similarity(GS)of the 16 cultivars through analysis of 115polymorphic RAPD loci obtained from 70informative primers were 53%～88%.The     

Cloning of Two Genes Related to Plant Defense Response ofSea Island Cotton (Gossypium barbaden.se L. )

Verticillium wilt,caused by V.dahaliae,is aserious fungus disease of cotton in China.Nearly all cultivated upland cotton(Gossypiumhirsutum)varieties are sensitive to it.Somespecies of island cotton(G.barbadense),however,have a natural resistance to thispathogen.To investigate the mechanism of SAR