纳米氧化铈颗粒对大鼠肺组织 GRP78表达的影响

探讨不同粒径纳米氧化铈颗粒对大鼠肺组织的毒性效应及对大鼠肺组织 GRP78蛋白表达的影响,将粒径分别为35 nm±3 nm、287 nm±27 nm 的纳米氧化铈颗粒悬液以400 mg／kg 剂量对大鼠灌肺。染毒28 d 后,采用免疫组化法检测大鼠肺组织 GRP78蛋白表达情况。结果表明,与对照组相比,不同粒径纳米氧化铈细胞 GRP78蛋白表达均增加（P ＜0．05）,且35 nm±3 nm 组高于287 nm±27 nm 组（P ＜0．05）。说明不同粒径纳米氧化铈均可引起大鼠肺组织 GRP78蛋白表达增加,且粒径越小,表达越高。     

无奇不有的鸡

营养丰富的鸡，是世人们饲养最多的一种家禽。但有些鸡与众不同，奇特有趣，鲜为人知。     

Study on the Activity Estimation Methods of Recombinant Chicken Interferon-α/Interleukin-2 Fusion Protein in vitro

In order to establish a stable,simple and rapid detection method to estimate the activities of the recombinant chicken interferon-α/interleukin-2 fusion protein (rChIFN-α-Linker-ChIL-2,recombinant fusion protein) in vitro,the activities of rChIFN-α-Linker-ChIL-2 were estimated by detecting its specific immune response to monoclonal antibody (MAb) against ChIFN-α and ChIL-2 by ELISA assay.The antiviral activities of rChIFN-α-Linker-ChIL-2 protein were tested by inhibiting the 50% appearance of cytopathic effect (CPE) of vesicular stomatitis virus (VSV) and infectious bursal disease virus (IBDV) on the passage cell lines DF1.The promoting proliferation activities of lymphocytes in the chicken peripheral blood and spleen of recombinant fusion protein were tested by MTS method.The results showed that rChIFN-α-Linker-ChIL-2 protein had the ability of specific immune response to anti-ChIFN-α MAb and anti-ChIL-2 MAb,respectively.The antiviral activity of rChIFN-α-Linker-ChIL-2 protein inhibiting the reproduction of VSV on DF1 cell line was higher than IBDV,and both of the antiviral activities of recombinant fusion protein against VSV and IBDV were much higher than the recombinant ChIFN-α protein (rChIFN-α) control.The recombinant fusion protein had apparent promoting proliferation activity of lymphocytes in the chicken peripheral blood and spleen,which were much higher than that of the rChIFN-α control.The study suggested that the activities detection and estimation methods of the recombinant fusion protein in vitro were successfully established,which laid the foundation for the further study of the synergy activity of recombinant fusion protein in vivo.     

Construction of Eukaryotic Expression Vector of Chicken MHCⅠα and β2m Genes and Its Expression in 293T Cells

To explore the mechanism of MHCⅠ molecule in immune response,chicken MHCⅠα and β₂m genes were cloned by PCR.Then the fragments were inserted into the eukaryotic expression vector with fluorescent protein,and the recombinant plasmids pEGFP-MHCⅠα and pmCherry-MHCⅠβ₂m were constructed.The recombinant plasmids were transfected into 293T cell with lipofectin reagent.The gene products of recombinant plasmids were mainly located to endomembrane system of the cells by fluorescence microscopy,and changed the intracellular localization of the fusion with the fluorescent protein.Moreover,the positive reactions were observed by the method of Western blotting,and the proteins had the molecular weight of 68.3 and 41.3 ku,respectively,in accord with the target proteins.The results showed that the recombinant plasmids were expressed in 293T cells with a good immunological activity,and the proteins had the binding reaction with specific antibodies.     

如何鉴别低产鸡和停产鸡

合理淘汰低产鸡和停产鸡 ,是提高产蛋率、降低饲料成本、增加养鸡经济效益的最有效措施之一。鉴别低产鸡和停产鸡的最科学的方法是根据鸡的个体产蛋记录进行 ,但在大群商业性生产鸡群中是不做个体记录的 ,因此只有从鸡的外貌特征和生理变化上进行鉴别 ,其常见方法如下 :(1 )产蛋鸡眼睛明亮有神 ,鸡冠、肉髯大而红润、富弹力 ,用手触之有温暖的感觉 ,开产后鸡冠倒向一侧。低产鸡一般眼神迟钝 ,鸡冠小而萎缩 ,苍白无光泽 ,以手触之有凉的感觉。(2 )产蛋鸡的肛门宽大 ,湿润、扩张形 ;停产鸡的肛门干燥而收小 ,无弹性。(3)高产鸡腹部容积大 ,触…     

略阳鸡与泰和鸡的肉质比较

<正> 我国用乌骨鸡配药治病历史悠久。当今以乌鸡为主要原料配制的中成药乌鸡白凤丸等疗效显著,享誉国内外市场。 略阳鸡多系乌皮,骨乌者较少。研究发现该鸡种体型较大(成年鸡平均体重2.62公斤),早期生长较快,与专门化肉鸡杂交效果显著,肉用潜力很大。但其药用价值如何,尚无研究资料。本研究旨在探讨略阳鸡的药用特性,为其选育和开发利用提供有益的参考。本文首先报告略阳鸡肉质分析结果。 一、材料与方法 1.试验动物 选取相同饲养条件下年龄相同的成年略阳鸡和泰和鸡测试。白羽略阳鸡、黑羽略阳鸡与泰和鸡各6只(3公,3母),     

成年鸡鸡白痢病例报告

鸡白痢是鸡的一种极其常见的传染病。在雏鸡往往表现为急性败血症的病型,发病率和死亡率都很高,通常在出壳后2周内死亡最多。在成年鸡多为慢性或隐性感染,一般不表现临床症状。但我国近年来发现育成鸡爆发鸡白痢的情况屡有发生,是一种新的发病动向,发病日龄在50～120日龄之间,应该在诊断中予以重视。我们最近发现一起成年蛋鸡的鸡白痢,引起严重损失。现将情况报道如下,以供参考。     

遗传性瘦肉鸡的质量

人们对肉食爱好中的明显趋势就是普遍厌恶脂肪,尤其厌恶瘦肉中的脂肪.鸡肉从这一趋势中大获益处,表现为其消费量相对上升。但这并不是说脂肪在鸡肉生产中不成为问题了.要降低脂肪含量,最好的办法就是要有低脂肪鸡种.在这方面,遗传选择是很成功的.选择的方法有好几种,包括对腹脂进行同胞选择和后裔测定以及对饲料转化率作群体选择.进行饲料转化率的选择,对于获取瘦肉鸡来说最为有效,同时鸡的产肉效率也最佳.然而选育瘦肉鸡不可降低鸡肉的食用质量.荷兰的 Spelderholt 中心研究了遗传性瘦肉鸡的生产、屠宰产量和食用质量。选择系在脂肪沉积率不同的鸡品系中比较了不同的瘦     

长沙黄鸡及其杂交组合肉质的研究

前言长沙黄鸡是湖南省培育的第一个优质黄羽型肉鸡,过去对其肉质进行了多次感观测验,其结果均证明长沙黄鸡具有较好的肉质,但是缺乏衡量的客观数据和指标。为此我们在1988年对长沙黄鸡及其杂种鸡进行了初步的分析,测定了酸度、肌纤维、肌肉营养成分、熟肉率、氨基酸含量等多项指标,现初报如下。     

猴源葡萄糖调节蛋白78生物信息学分析及其真核表达

试验旨在探究葡萄糖调节蛋白78（glucose regulatory protein 78,GRP78）基因的理化性质和结构特点,阐述GRP78在猪流行性腹泻病毒复制中的分子伴侣作用和调控机制。通过RT-PCR方法扩增GRP78基因,并插入到pMD20-T Simple载体进行克隆测序,利用生物信息学方法对其氨基酸序列、跨膜结构、糖基化位点、磷酸化位点、三级结构等进行预测和分析。将GRP78基因插入pCDNA 3.1中,然后转染Vero-E6细胞。Western blotting检测Vero-E6细胞中GRP78的表达。生物信息学分析结果表明,GRP78基因全长1 965 bp,编码654个氨基酸,蛋白质分子质量为72.33 ku,理论等电点为5.07,分子式为C₃₁₈₉H₅₁₅₃N₈₆₅O₁₀₁₉S₁₃。遗传进化树分析显示,猴源GRP78基因与双峰驼、犬、猫、大猩猩、蝙蝠、狒狒、猪、马的氨基酸序列同源性为99.5%~99.8%;遗传进化分析显示,大猩猩和狒狒亲缘关系最为接近。跨膜区和信号肽预测结果显示,该蛋白存在信号肽但不存在跨膜结构。GRP78蛋白无N-糖基化修饰位点,存在6个O-糖基化位点、28个磷酸化位点,表明GRP78可能有与激酶磷酸化有关的PKC、PKA特异性蛋白激酶的结合位点,可能参与己糖代谢和单糖代谢。     

淘汰低产鸡和仔产鸡的正规方法

一、根据外观淘汰低产鸡或停产鸡 看鸡冠 高产鸡的鸡冠丰满而鲜红，冠上部往往倒向一侧；而低产鸡和停产鸡的鸡冠萎缩，而且颜色发白，呈现淡红色或暗红色，表面附有麦麸样脱落物，有痂。     

珍珠鸡群暴发鸡白痢

1988年京郊某鸡场珍珠鸡群暴发鸡白痢,其病原为标准型的鸡白痢沙门氏菌。介绍于下。一、发病情况1987年底京郊某鸡场从南方某地引进珍珠鸡1000只(公:母=1: