浅析犬瘟热、犬细小病毒病的诊治

犬病毒性传染病包括犬瘟热、犬细小病毒病、犬传染性肝炎、狂犬病、犬冠状病毒病、犬疱疹病毒病、犬轮状病毒病、犬副流感、伪狂犬病等.下面笔者主要对临床中最为常见,且发病率、死亡率高,对犬类危害严重的犬瘟热、犬细小病毒病这两种病毒性传染病的诊断及防治作一介绍.     

犬瘟热病的诊断与防治

犬瘟热是犬最重要的病毒性传染病,遍及全世界。感染肉食动物中犬科,尤其是对幼犬危害最为严重,有高度传染性、致死性。加强对犬瘟热的诊断与防治对提高养犬经济效益有着重要的意义。本文将对犬瘟热病的流行病学特点、临床特征、病理变化及防治措施做一探索,意在减少疾病带来的危害。     

犬瘟热病毒检测方法的研究进展

犬瘟热病毒是犬瘟热的病原体,属副粘病毒科、麻疹病毒属成员,是危害犬类健康最严重的病原之一,给养犬业带来巨大的经济损失,而采取早期诊断和对症治疗是预防犬瘟热的有效措施。本文对近些年国内外有关犬瘟热病毒检测技术的新进展予以了综述,期待为诊断、预防和控制犬瘟热的流行和蔓延提供参考。     

犬瘟热的诊疗及预防

<正>犬瘟热,俗称狗瘟,是由犬瘟热病毒所致的犬和野生食肉兽的一种急性、败血性传染病。犬瘟热在我国发病率和死亡率有不断上升的趋势,是危害养犬业比较严重的疫病之一,所以对于养犬业来说犬瘟热的诊断和防治意义尤为重要。     

犬常见病毒性传染病的诊断及治疗

犬的传染病有病毒性、细菌性和真菌性三大类,但最常见且危害最大的是病毒性传染病,包括犬瘟热、犬细小病毒性肠炎、犬冠状病毒性肠炎、犬传染性肝炎、犬传染性喉气管炎和狂犬病,其中以犬瘟热、犬细小病毒、犬传染性肝炎、犬冠状病毒,发病率及死亡率最高,治疗有一定难度,直接影响养犬业的发展。下面将对犬常见几种病毒性传染病的诊断要点及治疗方法做一介绍。     

犬常见病毒性传染病的诊断及治疗

犬的传染病有病毒性、细菌性和真菌性三大类,但最常见且危害最大的是病毒性传染病,包括犬瘟热、犬细小病毒性肠炎、犬冠状病毒性肠炎、犬传染性肝炎、犬传染性喉气管炎和狂犬病,其中以犬瘟热、犬细小病毒、犬传染性肝炎、犬冠状病毒,发病率及死亡率最高,治疗有一定难度,直接影响养犬业的发展.下面将对犬常见几种病毒性传染病的诊断要点及治疗方法做一介绍.     

犬瘟热病的临床诊疗概述

<正>犬瘟热是由犬瘟热病毒引起的一种急性高度接触性传染病,对我国养犬业、毛皮动物养殖业和野生动物保护业危害巨大。我国近年来发病率有增长的趋势。本文对其临床诊断与治疗进行了综述。1犬瘟热病因犬瘟热(caninedistemper,CD)是由犬瘟热病毒(CDV)引起的犬科、鼬科和熊科动物的一种高度接触性传染病,是当前我国对养犬业、毛皮动物养殖业危害最大的疫病之一。犬瘟热病毒为副粘病毒科麻疹病毒属,呈圆形或     

犬瘟热的诊断及防治措施

犬瘟热(canine distemper)是养犬业危害最大的传染病之一,发病率高,死亡率高。怎样采取预防措施,对病犬及早确诊、正确治疗,以减少经济损失,是当前困扰养犬业发展的一道难题。研究犬瘟热的病原及流行特点、症状及诊断,对于治疗与防患有着重要意义。     

犬瘟热的诊断技巧及防治措施

犬瘟热(canine distemper)是养犬业危害最大的传染病之一,发病率高,死亡率高。怎样采取预防措施,对病犬及早确诊、正确治疗,以减少经济损失,是当前困扰养犬业发展的一道难题。研究犬瘟热的病原及流行特点、症状及诊断,对于治疗与防治有着重要意义。     

犬常见传染病的诊断及防制

近几年来,城乡养狗的数量不断增加,狗的传染病也随之频繁发生,尤以犬瘟热、犬细小病毒为主的病毒病,给人类健康和畜牧业发展带来极大危害.现将犬瘟热、犬细小病毒的发生与传播途径、诊断要点、防治措施做以阐述,以及当前对犬瘟热、犬细小病毒病应采取的控防措施.     

兰州地区犬瘟热流行病学调查及其治疗

为了解兰州地区犬瘟热发病率、死亡率与犬年龄、品种、免疫接种、季节之间的关系,并总结出一套治疗犬瘟热最有效的方法,收集108例临床犬瘟热病例,从流行病学、临床鉴别诊断、病理剖检、治疗等方面进行深入、全面的研究。结果表明,犬瘟热发病率及死亡率与犬的年龄、品种、免疫接种、季节有很大的关系;通过应用被动免疫（犬瘟热血清、犬瘟热单克隆抗体）联合主动免疫（弱毒疫苗）的治疗方法取得了良好的治疗效果。     

犬瘟热的诊疗及血细胞变化监测

患犬经临床检查和实验室检验确诊为犬瘟热.综合采用抗病毒、抗菌、对症治疗和支持疗法,并对诊疗过程中患犬的血细胞变化进行了监测;患犬康复后留有抽搐后遗症.     

郑州市犬瘟热病毒感染血清学调查

为了解郑州市犬瘟热流行情况,为犬瘟热的预防提供参考依据。采用间接ELISA法对来自郑州市480份血清进行抗体水平的检测,结果显示480份犬血清中犬瘟热抗体平均阳性率为16.04%,其中3岁以下犬犬瘟热阳性率为13.13%,3岁以上犬犬瘟热阳性率为 2.91%。犬瘟热的发病率仍然很高,尤其是3岁以下的犬犬瘟热阳性率较为高,应引起高度重视,防止犬瘟热的传播。     

Juvenile cellulitis in an 8-month-old dog

An 8-month-old, crossbred dog, presented with a painful, swollen face. The problem was of 4 weeks duration and had not responded to antibiotics. A diagnosis of canine juvenile cellulitis was based on the clinical presentation and supportive cytological and histopathological changes. Attempts to identify canine distemper virus were not successful. Complete resolution occurred with glucocorticoid therapy. This is the first report of canine juvenile cellulitis in a dog of this age.     

Cell-mediated immunity and age at vaccination associated with measles inoculation and protection of dogs against canine distemper.

The antibody-mediated immune response (AMIR) of dogs to measles and canine distemper viruses has been described. However, there is little information on the cell-mediated immune response (CMIR). The AMIR and the CMIR of dogs to canine distemper and to measles were examined. The CMIR was determined for 6 weeks by measuring the 3H-thymidine uptake by immune lymphocytes in the lymphocyte transformation test. Concurrently, canine distemper and measles virus serum-neutralization antibodies were measured by a microtitration serum-neutralization test. Dogs vaccinated with canine distemper virus had a CMIR and an AMIR to canine distemper. However, measles virus-vaccinated dogs had only a CMIR to canine distemper. A CMIR in the absence of an AMIR indicates that cell-mediated immunity is the most important immune mechanism in protecting measles virus-vaccinated dogs against canine distemper. Development of CMIR and AMIR to canine distemper and measles antigens depended on the age of the dog at the time of vaccination. Adult and juvenile dogs had immune responses to both canine distemper and measles. Neither virus, however, elicited an immune response in neonates.     

RT-PCR检测宠物犬的犬瘟热病毒

为了寻找一种在早期快速准确检测宠物犬犬瘟热病毒(CDV)的方法,从临床上疑似CDV感染犬抗凝血中分离出血淋巴细胞,从中提取总RNA,引用曾在警犬上成功检测到CDV的引物序列,反复摸索RT-PCR反应条件,经凝胶电泳观察到在理论设计处(287 bp)出现了光亮条带,说明已成功地从宠物犬中检测出CDV。表明该引物序列和改良的RT-PCR反应条件适合于检测宠物犬CDV感染。     

Research Progress on Diagnostic Technique of Canine Distemper

In recent years, canine distemper is a serious damage to health development of dog and fur animal breeding industry.Fast and accurate diagnostic technique is particularly important for canine distemper prevention.At the present stage, canine distemper diagnostic techniques mainly include virus isolation, culture and microscopy observation, enzyme-linked immunosorbent assay(ELISA), immunohistochemistry, immune colloidal gold technique, nucleic acid hybridization technique, nested RT-PCR, Real-time fluorescence quantitative RT-PCR, double PCR, gene chip technology, liquid phase chip technology, loop-mediated isothermal amplification technology, and so on.The key of isolation, culture and microscopy observation of canine distemper virus is to obtain a susceptible cell lines that can produce typical CPE.We review research progress on diagnostic techniques of canine distemper, so as to provide simple and highly effective detecting technique for preventing and controlling epidemic and outbreak of canine distemper.     

犬瘟热诊断技术的研究进展

近年来,犬瘟热已严重危害了犬及毛皮动物养殖业的健康发展,快速、准确的诊断技术对于犬瘟热的预防显得尤为重要。现阶段犬瘟热诊断技术的研究主要有病毒分离培养与镜检观察、酶联免疫吸附试验(ELISA)、免疫组化、免疫胶体金、核酸杂交、巢式RT-PCR、实时荧光定量RT-PCR、双重PCR、基因芯片、液相芯片、环介导等温扩增等。获得能产生典型细胞病变的易感细胞株是成功进行犬瘟热病毒分离培养与镜检观察的关键。作者就犬瘟热诊断技术的研究进展作一综述,为有效预防和控制犬瘟热的流行和暴发提供简便、高效的检测技术。     

Optic neuritis caused by canine distemper virus in a Jack Russell terrier

An atypical case of canine distemper (CD) was diagnosed in a vaccinated healthy adult dog. The patient was presented circling, seizuring, and blind. Postmortem examination resulted in a diagnosis of CD. Optic neuritis was diagnosed, a finding not previously described in the context of CD virus infection presenting solely with neurological signs.     

一株貉源犬瘟热病毒的分离与鉴定

从临床疑似犬瘟热的病貉组织中分离到1株病毒,经形态特征、血凝试验、中和试验、间接免疫荧光试验和分子生物学鉴定,该分离病毒为犬瘟热病毒。本研究为研究犬瘟热病毒貉株分子生物学特性、致病机理及构建基因工程疫苗奠定了基础。