一起生长肥育猪发生猪伪狂犬病的诊治

2013年11月,江西某规模化猪场生长肥育猪陆续发病,表现为体温升高（40.5～41℃）、咳嗽、腹式呼吸、流涎、流脓鼻涕,部分猪表现神经症状（原地转圈、步态不稳）。经临床诊断、实验室PCR检测以及家兔感染试验,确诊为猪伪狂犬病,现将该病的诊治情况报告如下。     

“猪地方性肺炎及其防治控制”专栏五：生长/肥育猪爆发气喘病时的全面应对措施

2003年10月,加拿大马尼托巴南部一个猪肺炎支原体阴性商品猪生产系统的生长/肥育猪生产点爆发了猪地方性肺炎。该生产系统的应对措施包括药物拌料、空棚、推迟猪转入感染点的时间、给处于感染危险中的母猪群接种疫苗,并在生长/肥育猪生产点空棚后进行彻底消毒。     

猪气喘病（猪地方流行性肺炎）的诊断与防治

猪气喘病是由肺炎支原体引起猪的一种慢性、接触性传染病。主要临诊症状是咳嗽和喘气。剖检变化为肺的尖叶、心叶和膈叶的对称性实变。以及肺门淋巴结增生。病多呈慢性经过．常有其他病菌继发感染。     

猪大叶性肺炎的诊断及防治

大叶性肺炎是整个肺叶发生的急性炎症过程,有纤维蛋白渗出。因此,又称为纤维素性肺炎或格鲁布性肺炎。临床上以高热稽留、铁锈色鼻液、大片肺浊音区、支气管和肺泡内充满大量纤维蛋白渗出的为主要特征。1临床症状突然发病,体温迅速升高到40℃～41℃,甚至更高,并稽留6～9d,以后渐退或骤退至常温。患猪表现精神沉郁,食欲减退或废绝,但脉搏加快不明显。高热而脉搏不太快是本病早期的特征。呼吸急促,每分钟可达60次,呈混合性呼吸困难。粘膜发绀、黄染,皮温不整,肌肉震颤。患猪频发短痛咳,溶解期变为咳,肝变初期,流铁锈色或黄红色鼻液。重症病例,…     

通过对猪支气管肺泡灌洗液糖蛋白的分析显示出诊断猪胸膜肺炎放线杆菌的潜在标志物

德国汉诺威动物医科大学的科学家们研究了德国长白、皮特兰和汉普夏3个品系猪对胸膜肺炎放线杆菌（Actinobacillus pleuropneumoniae）的抗性。首先给猪作攻毒实验,然后从急性感染后4d、慢性感染后20d和健康猪分别采集支气管肺泡灌洗液（bronchoalveolar lavage fluid,BALF）,并用先进的蛋白质组学技术对BALF中的糖基化蛋白表达量作比较分析。     

猪气喘病的类型诊断和防制

猪气喘病,又称猪地方流行性肺炎或猪支原体肺炎。是猪的一种高度接触性慢性呼吸道传染病,广泛分布于世界各地。其主要症状是咳嗽和气喘,生长发育缓慢,病变特征是融合性支气管肺炎,肺尖叶、心叶、中间叶和膈叶前缘呈“肉样”或“虾肉样”实变。本病在暴发流行的初期或饲养管理不善,如有继发性病原体感染,可造成大批死亡,严重威胁着现代养猪业的发展。本病的病原是猪肺炎支原体,无细胞壁,呈多形性,革兰氏染色阴性,对培养条件要求严格,对外界抵抗力不强,常用化学消毒剂均能杀灭。猪肺炎支原体对青霉素及磺胺类药物不敏感,对四环素类、泰妙菌素…     

猪气喘病的诊断及防治方法

猪气喘病又称猪地方流行性肺炎,是由肺炎支原体引起的猪的一种慢性接触性传染病。猪气喘病是规模猪场常见的疫病之一,也是猪场要求净化的主要疫病。近年来,该病在我乡部分规模猪场时有发生,特别是在一些饲养太湖母猪的规模猪场发病较重,给养殖户造成了很大的烦恼和一定的经济损失。现将其流行特点、主要症状和防治措施报告如下。     

猪口蹄疫与猪水泡病的诊断区别

1病原区别口蹄疫病毒(FMDV)属于小核糖核酸病毒科,口蹄疫病毒属,病毒无囊膜。PH值6.7～10.5,表现稳定。有7个血清型,A型;O型;C型;南非(SAT)Ⅰ、Ⅱ、Ⅲ型;亚洲Ⅰ型。多变种或亚型存在于每个血清型里。水泡病病毒(SVDV)属于小核糖核酸病毒科,肠道病毒属,病毒无囊膜。PH值3～5,表现稳定,对环境抵抗力较强,50℃、30分钟仍不失感染力。2流行病学区别     

猪附红细胞体特异性基因的克隆和PCR诊断方法的建立

根据2003年Hoelzle发表的猪附红细胞体的基因组序列（AJ504999）设计一对特异性引物,对病料样品进行PCR扩增并将其产物克隆到pMD18-T载体后测序,结果表明扩增出的片段为603bp,同源性分析表明该序列与参考基因组序列同源性为100％,反映出我国分离株与国外株其基因无差异,特异性和敏感性试验表明,所建立的PCR诊断方法与常见的支原体、细菌及原虫无交叉反应,能检测到猪附红细胞体血液基因组DNA最低量为0．65ng／mL,该方法具有快速、特异、敏感等特点,为猪附红细胞体病的快速诊断及流行病学调查提供了新的手段。     

猪断奶后多系统衰竭综合征诊断方法及标准的建立

猪断奶后多系统衰竭综合征(PMWS)是一种由猪圆环病毒2型(Porcine circovirus 2，PCV2)引起的新病。PCV是1974年在猪肾传代细胞系PK-15中发现的一种污染病毒，该病毒不产生细胞病变效应(CPE)，无致病性，命名为PCV1；而后在PMWS中分离的猪圆环病毒，有致病性，命名为PCV2。PMWS主要感染1～5月龄的猪，发病率为4％～30％，致死率为70％～80％。患病断奶猪和生长猪临床表现为进行性消瘦、呼吸困难、皮肤苍白、黄疸、腹泻和体表淋巴结肿大，     

Value of the clinical examination in diagnosing enzootic pneumonia in fattening pigs

The diagnosis of enzootic pneumonia at the herd level should be based on a combination of different methods. Currently, clinical examination is usually considered to be a low value method, particularly compared to the direct detection of Mycoplasma hyopneumoniae in lung lesions by PCR. The present study compared the value of accurate clinical examination (including the quantitation of coughing), PCR on bronchoalveolar lavage fluid, and serological testing of blood samples for the purpose of diagnosing enzootic pneumonia. The coughing index (average % of pigs coughing per minute of observation) was determined in fattening pigs from 59 herds, and ranged from 0% to 6.7% with a median of 2.4%. Five hundred and ninety bronchiolar lavage samples and 1179 serum samples were taken from pigs in those 59 herds and tested for M. hyopneumoniae specific DNA and antibodies, respectively. In herds where ?50% of lavage fluids were PCR positive, the likelihood of a higher coughing index was increased by 76% (OR: 1.76; 95% CI: 1.14-2.72) compared to herds with <50% of positive samples. For antibodies (determined by ELISA) a seroprevalence of ?50% increased the likelihood of a high coughing index by 50% (OR: 1.50; 95% CI: 1.03-2.20). In 78.1% of all herds with a seroprevalence of ?50% against M. hyopneumoniae, the PCR-prevalence and the coughing index were above the median (50% and 2.4%, respectively). It was concluded that in fattening pigs a quantitative assessment of the onset of coughing - typically dry and non-productive - improves the diagnosis of enzootic pneumonia and can occasionally substitute for the detection of M. hyopneumoniae by PCR.     

The synergistic activity of tiamulin and chlortetracycline: in-feed treatment of bacterially complicated enzootic pneumonia in fattening pigs

The antibacterial effects of a combination of tiamulin and chlortetracycline in vitro against a number of field isolates of Pasteurella multocida, Haemophilus pleuropneumoniae and Bordetella bronchiseptica were examined. There was a marked synergism between the two antibiotics against all eight isolates of P multocida, against seven of nine isolates of H pleuropneumoniae and against the single strain of B bronchiseptica tested. Two field trials were carried out on a herd with a history of complicated enzootic pneumonia where the presence of Mycoplasma hyopneumoniae and P multocida had been established and subsequently the presence of H pleuropneumoniae was discovered. Feed containing tiamulin at 100 ppm combined with chlortetracycline at 300 ppm was given for seven days to pigs affected with pneumonia, and the results were compared with untreated controls and pigs receiving chlortetracycline at 300 ppm. There was a follow-up observation period of three weeks when all groups received unmedicated feed. During the medication period the combination treated groups showed a statistically significant increase in average daily weight gain of 156 g (20.4 per cent) and in feed conversion efficiency of 0.576 (20.8 per cent) and a numerical improvement in average disease score in comparison with the untreated controls. These improvements were approximately double those observed in the groups treated with 300 ppm chlortetracycline which showed improvements of 93 g (12.2 per cent) in average daily gain and 0.301 (10.9 per cent) in feed conversion efficiency. During the following three weeks most of the initial gains were lost, probably owing to the reinfection of the treated groups by the untreated controls.     

A system response to an outbreak of enzootic pneumonia in grow/finish pigs

A Mycoplasma hyopneumoniae-negative commercial swine production system broke with enzootic pneumonia at their grow/finish site in southern Manitoba in October, 2003. System responses included feed medication, depopulation, delayed shipment of pigs to the infected site, vaccination of at risk sow herds, and disinfection when grow/finish site depopulation was completed.     

生长/肥育猪爆发气喘病时的全面应对措施

2003年10月,加拿大马尼托巴南部一个猪肺炎支原体阴性商品猪生产系统的生长/肥育猪生产点爆发了猪地方性肺炎.该生产系统的应对措施包括药物拌料、空棚、推迟猪转入感染点的时间、给处于感染危险中的母猪群接种疫苗,并在生长/肥育猪生产点空棚后进行彻底消毒.