兰科植物种子表面消毒方法及其对种子萌发率的影响

兰花的种子夹在贮藏时期易于开裂而使种子污染,因而不适用于无菌的组培作业。为探究合适兰科植物种子的表面消毒方法,并将其应用到无菌萌发研究及生产之中,以兰科植物贵州地宝兰(Geodorum eulophioides)的种子为研究对象,利用一种尼龙网种子袋对兰科植物微小种子进行了表面消毒方法比较的研究。在试验中比较了不同消毒剂及不同消毒时间对种子萌发率的影响。结果表明,75%酒精消毒30 s会使贵州地宝兰种子失去活性,不适用于其表面消毒;2% NaClO溶液的消毒效果与消毒时间成正比,当消毒时间增加为6 min时没有污染,消毒效果较好,种子萌发率达到33.88%,且其对贵州地宝兰种子萌发率后续生长无影响,因此是一种有效的表面消毒方法,可适用于兰科植物种子无菌萌发的研究及生产实践。     

小鼠不同组织中催乳素释放肽 受体基因表达的研究

【研究目的】克隆并研究小鼠催乳素释放肽受体基因在妊娠期小鼠不同组织的表达【方法】提取小鼠不同组织总RNA,进行RT-PCR,PCR产物与pMD-19T连接后转化E.coli DH5a,检测阳性克隆并测序【结果】测序结果表明,所得序列与小鼠催乳素释放肽编码区序列的相似性为99.49%【结论】克隆所得序列为小鼠催乳素释放肽受体基因片段,催乳素释放肽在妊娠期小鼠不同组织均有表达     

火龙果总DNA提取方法比较研究

摘要：火龙果组织中含有多糖及其他次生代谢物质,因而难以提取高质量的DNA。本试验以火龙果嫩茎为材料,进行多种DNA提取方法比较研究,并通过琼脂糖凝胶电泳对所提取的DNA样品进行检测,结果表明,改良CTAB法和微量CTAB法均可用于火龙果DNA提取,尤其是微量CTAB法,提取的DNA纯度较为理想,能满足后续分子生物学研究的要求。     

补光对烟苗生长及主要生理指标的影响

为解决湖南冬季大棚育苗低温寡照的障碍,研究光照强度对烟苗生长及主要生理指标的影响。在水温恒定条件下分别采用LED蓝:红=1:1的配比组合灯补光（补光强度分别为3000、4500、6000 lx）,结果表明：随光照强度的增加,烟苗的出叶天数减少,而叶片生长速率加快,各处理存在显著差异;补光强度达到6000 lx,烟苗茎粗,茎高、干鲜重和干鲜比大,幼苗干物质积累最多;质体色素和根系活力受光照强度影响较大,其中叶绿素a、叶绿素b、类胡萝卜素和根系活力随光照强度增强而升高,而叶绿素a/b比值减少,表明补光有利于培育烟草壮苗,可以提高成苗素质。     

不同施肥量对烤烟产量与质量的研究

施肥是烤烟栽培的重要环节之一，肥料品种及不同用量直接影响到烤烟的产量与品质。烤烟施肥是人们研究得较多的问题，已有许多报道，但在不同地区不同生态条件下的烤烟施肥仍就是新课题，为此于1996，1997年根据省科委下达的《遵义地区烤烟大面积优质高产综合配套技术研究与应用》课题要求，对桐梓县的烤烟不同施肥量进行研究，目的在于探索该地区的烤烟最佳施肥量，为提高烟叶产量和质量，增加上中等烟比例提供施肥理论依据。1材料与方法本试验在桐样县的茅石乡新桥片区进行。按一方案在同一块土进行两年，供试地块为黄泥，肥力中等均匀，…     

黑粒小麦的选育及其抗氧化特性

摘要：本文选育的黑粒小麦为黑皮黑胚乳,含有丰富的水溶性黑色素的小麦,国内外对黑色素作了一定的开发研究,但黒粒小麦清除二苯基-2-苦肼基自由基的研究还很少。本研究以普通小麦和抗坏血酸为对照,通过DPPH 法测定黑粒小麦色素粗提物体外抗氧化、清除自由基能力。研究结果表明:黑粒小麦黑色素粗提物具有呈量效关系的抗氧化活性,其清除自由基能力远大于普通小麦,但小于抗坏血酸。普通小麦、黑粒小麦3-2、3-3、3-4、3-11、3-13、3-33、3-36、3-37、3-39、10-38和Vc清除50% DPPH 自由基的有效浓度（EC50）分别为689.05、39.89、71.51、58.57、48.13、33.99、105.27、62.15、30.97、35.15、35.09、0.196 mg/ml,其自由基清除能力AE 分别为0.15×10-2、2.51×10-2、1.4×10-2、1.7×10-2、2.08×10-2、2.94×10-2、0.95×10-2、1.61×10-2、3.23×10-2、2.84×10-2、2.85×10-2、510×10-2。这些为进一步研究、开发和利用黒粒小麦黑色素奠定理论基础。     

蝴蝶兰组织培养体系的建立及其关键技术研究

以蝴蝶兰为供试材料,采用幼嫩的花梗、开花后的花梗和茎尖作为外植体,探索蝴蝶兰组织培养技术体系,着重研究各阶段的最佳培养基培养及培养程序。结果表明,开花后的花梗是诱导芽的良好材料,适合花梗诱导芽的培养基是MS+BA1mg/L+NAA0.1 mg/L。防止茎尖褐化的方法是将1%VC过滤灭菌后倒入培养皿,在培养皿中切取茎尖,接种到添加了0.2%PVP的培养基上,适宜茎尖诱导芽的培养基是VW+ BA3mg/L+NAA0.5 mg/L+椰汁200ml/L。适合增殖的培养基是MS+ BA1mg/L+NAA1 mg/L。适合生根的培养基是?MS +IBA1mg/L+NAA1 mg/L。生根的组培苗,置于温室进行经过适当的炼苗处理,移栽基质为湿润海藻,环境湿度保持在80%以上。     

含磷农用保水剂的制备及性能研究

【研究目的】为了修复土壤墒值,缓解干旱对我省作物和林木的影响,制备一种含磷农业保水剂;【方法】采用水溶液聚合法,以磷尾矿粉作为主要添加量,以N,N'-亚甲基双丙烯酰胺为交联剂,过硫酸铵为引发剂,丙烯酰胺（AM)和部分中和的丙烯酸(AA)为单体;【结果】经实验优化,当聚合温度为75oC,单体中和度为60%,丙烯酸浓度为15%,AA与AM质量比为4：1,引发剂用量为单体质量的0.5%,交联剂用量为单体质量的0.02%,磷尾矿粉添加量为10g时制备的保水剂,其吸蒸馏水量为621g/g,吸盐水量为52.71g/g;【结论】通过本实验,研制出一种含磷农用保水剂,除具有保水复墒作用外,还可以累计释放磷元素,帮助作物生长的同时,还能缓解磷尾矿粉对环境造成的压力。     

混合碱胁迫对碱茅和紫花苜蓿种子萌发的影响

采用室内种子萌发实验方法,以碱茅(Puccinellia distans),紫花苜蓿(Medicago sativa L)两种牧草种子为研究对象,以NaHCO₃和Na₂CO₃为基础盐分,将NaHCO₃ 和Na₂CO₃按照1:0,2:1,1:1,1:2,1:1的比例配成相同浓度的复合溶液,研究不同混合盐分胁迫对碱茅和紫花苜蓿种子发芽率、发芽势、发芽指数、芽长和芽重的影响。结果表明：不同比例的混合盐分胁迫对碱茅和苜蓿种子的发芽率、发芽势、发芽指数和芽长均有明显地抑制作用。但是,不同盐分组合处理间对碱茅和紫花苜蓿的芽重的影响比单一盐分要小。单一盐分比较,NaHCO₃的抑制作用更强。NaHCO₃ 和Na₂CO₃的比例为1:1时,对两种种子的发芽抑制作用最强。两种牧草比较,Na₂CO₃ 和NaHCO₃混合盐分胁迫对碱茅种子萌发的抑制作用较弱,对紫花苜蓿种子萌发的抑制作用较强。     

龙眼采后贮藏过程中多胺含量的变化

以福眼龙眼为试材,研究了果实中多胺类型,以及随贮期延长,果皮和果肉中腐胺、亚精胺和精胺3种多胺的变化情况。研究结果表明：室温贮藏下,果皮的3种多胺含量均不断下降,而果肉中只有腐胺含量下降,精胺和亚精胺含量变化不明显;低温贮藏下,果皮的内源多胺在采后前期迅速下降,接着保持在较稳定水平,而果肉中只有腐胺含量跟果皮的变化趋势一致,精胺和亚精胺含量变化不明显;且果肉中的多胺含量明显高于果皮。     

BIM Based on Intelligent Parametric Modeling Technology

In order to push the informationization process of the building industry,promote sustainable architectural design and enhance the competitiveness of China's building industry,the authors study BIM(Building Information Modeling) based on intelligent parametric modeling technology.BIM is a new technology in the field of computer aided architectural design,which contains not only geometric data but also the great amount of engineering data throughout the lifecycle of a building.The authors also compare BIM technology with 2D CAD technology,and demonstrate the advantages and characteristics of intelligent parametric modeling technology.BIM,which is based on intelligent parametric modeling technology,will certainly replace traditional computer aided architectural design and become a new driving force to push forward China's building industry in this Information Age.     

Innovation of Construction Projects Management Based on the Five-dimension Modeling

Nowadays,the computer technology is applied more and more widely in the architecture,engineering and construction industries(AEC).This paper introduces a methodology of construction project management based on the five-dimension modeling which integrates three-dimension building models,resources and schedules by the development of virtual construction technology.The application of this method also influences several aspects of project management such as range,time,cost,resource,communication and information management.Finally,the process of 5D modeling and the management practice are presented through a real life case in Beijing which proves to be a more efficient,productive and economy project.     

OpenGL在粮仓温度场可视化中的应用

根据粮仓温度监测系统采集到的温度数据,首次在VC 6．0MFC平台上,运用3DSMAX,OpenGL图形工具包,实现了整个粮库外景三维建模以及粮仓温度场的动态三维重现。从而可直观地了解粮仓内部的温度情况。     

计算机视觉技术在水稻氮素营养诊断中应用的研究进展

为探究水稻冠层外观特征与水稻氮素营养状况的关系,综述了应用计算机视觉技术进行水稻氮素营养诊断的基本思路及研究进展。主要分述了常规性水稻氮素营养指标的获取,水稻冠层图像的获取、预处理以及特征的提取与优化,水稻氮素营养诊断模型建立的方法等方面的内容。指出近地面水稻冠层图像的获取方法、水稻图像处理方法、多种综合性特征的优化选择方式都有待进一步研究,应用机器学习进行水稻氮素营养诊断建模的方法需要更加深入探究。今后应加大计算机视觉技术在水稻氮素营养诊断相关研究中的应用,将多种图像处理方法、特征优化选择方法与机器学习建模方法相结合,并探寻更为简便易行的方法进行水稻氮素营养诊断。     

基于Quest3D的虚拟园林漫游系统的设计与实现

摘要：目前,虚拟现实技术在园林中的应用尚处于探索时期,但随着计算机软硬件水平的提高和可视化技术的不断发展更新,虚拟现实技术在园林中应用的领域逐渐广阔,对园林设计的影响也越来越大。本文以一个城市广场的园林设计为例,结合Quest3D技术制作开发了一个园林漫游展示系统。介绍了园林漫游展示系统的设计过程,研究了园林场景的建模、植物和水体模型的控制、光照和阴影设置、摄像机设置等问题。探讨了Quest3D技术在园林设计中的应用,研究了园林交互展示的相关技术,总结了构建园林漫游展示系统的方法和过程,为园林设计的展示提供一种有效的方法和途径。     

Simulation on human-machine cooperation strategy in production cells

To adapt to the rapid changes of manufacturing environment,production cell needs to focus on high qualified staff and flexible human-machine cooperation method at the same time,when advanced manufacturing technology(AMT) is emphasized on. In order to study human-machine cooperation strategy dynamically and quantitatively in production cells,a simulation study which combined computer simulation with agent modeling technology is carried out. Firstly,a human-computer cooperation simulation model is established,and its realization method is presented. Secondly,specific cooperation mechanisms of designated production cell cooperation,partly independent cooperation and completely independent cooperation are studied respectively. Finally,an engine production cell is taken as an example,a series of simulation experiments are designed,and four performance indicators of the task waiting time,order on time percentage,waiting rate of machine and operator utilization are established to analyze and evaluate all kinds of man-machine division modes of the production cell to find a more reasonable mode.     

Twisted Blade Virtual Design for Steam Turbine

Due to lack of estimation during initial stage of traditional physical design process for industry product, the efficiency of conduct development is low with high cost. A basic framework of twisted blade virtual design is set up based on the theory and method of product virtual design, and aiming at the thermodynamic process of middle or lower stage of steam turbine. The molding rule of twisted blade is found by thermodynamic computation program. The study of blade virtual design is carried through from 3-dimensional modeling to simulation test. The combination of thermal power theory, computer simulation technology and CAD system forms a kind of design method with the superiority such as high efficiency, low cost and good decidability is represented in the virtual design of turbine blade.     

Application of GDL Computer Technology in the Field of Architecture Informationization

In the Information Age, an urgent problem facing the present-day architecture informationization in China is how to effectively utilize new computer technology to improve production efficiency in the building industry. GDL computer technology is a new technology designed for developing smart and parametric building models. By making an in-depth study of GDL technological theories and its fundamental features and taking into consideration the status quo of its application, the authors put forward the development and application modes of GDL computer technology in the field of architecture informationization and analyze specific solutions as well as its prospects, so as to provide the theoretical basis for and solutions to accelerating the architecture informationization process and offer a new technological means of enhancing productivity in the building industry.