Enzymes as silage additives 2. The effect of grass dry matter content on silage quality and performance in sheep

Eight grass silages were made from two contrasting varieties of perennial ryegrass, four silages each from a late-cut early-maturing (high dry matter, HDM) variety and from an early-cut, late-maturing (low dry matter, LDM) variety. The grass was ensiled without additive (untreated), or with formic acid, or with one of two enzyme mixtures of hemicellulases and cellulases (enzyme 1 and enzyme 2) for a period of 130 d.
Formic acid-treated silage had lower levels of lactic acid at both levels of dry matter than the other silages. Enzyme treatment of grass prior to ensilage resulted in reduced levels of cellulose, acid-detergent fibre and neutral-detergent fibre in LDM silages and lower acid-detergent fibre and neutral-detergent fibre in the HDM silages compared with the corresponding untreated and formic acid-treated silages. Voluntary intakes (g DM d⁻¹) of untreated and enzyme-treated silages were significantly ( P <0·01) lower at both digestibilities compared with formic acid-treated silages (LDM: untreated, 982; formic, 1069; enzyme 1, 868; enzyme 2, 937; HDM: untreated, 931; formic, 1027; enzyme 1, 943; enzyme 2, 914). The organic matter, carbohydrate and nitrogen digestibility coefficients of LDM silages were significantly ( P <0·001) higher than those of HDM silages. There were no significant differences in any component digestibility related to silage additive.
Comparison of digestibility coefficients for constituents of the LDM silages fed to sheep or steers showed no differences between species.     

The effect of dry feed additives on the nutritive value of silage

Silages were prepared in late September from a mixed crop of perennial ryegrass and white clover with low dry matter and high nitrogen content. Six different treatments were used during ensiling; addition of either molassed sugar beet pulp or rolled barley, at 50 kg and 25 kg (t fresh grass)^?1, formic acid at 51 t^?1 and no additive (control). All silages were well fermented with low levels of ammonia and pH. The addition of rolled barley or sugar beet pulp increased the dry matter content of the silages incrementally and appeared to assist the retention of nitrogen in the silage. Dry matter intakes of silages with 50 kg of barley or sugar beet pulp tonne^?1 were similar to the control silage when fed to wether sheep but at 25 kg t^?1, dry matter intake increased by 0·15 with barley and 0·04 with sugar beet pulp compared to the control. The digestibility of dry matter (DM) and organic matter (OM) were significantly higher than the control for silages treated with formic acid or high levels of barley and sugar beet pulp whereas neutral-detergent fibre (NDF) digestibility did not appear to be significantly affected. All treated silages had a significantly higher metabolizable energy (ME) content than the control, and additions of barley or sugar beet increased the ME content. The ME intakes of all treated silages were also significantly greater than those of the control, and the formic acid-treated and low barley silages were the highest. This appeared to be associated with significantly higher energy digestibility and DM intake. Addition of rolled barley during ensilage resulted in greater nitrogen intake, availability and retention compared to additions of sugar beet pulp. The higher retention with barley silages was associated with a significantly lower proportional loss of absorbed nitrogen in urine, which indicated a more efficient utilization of nitrogen. These differences require further study to determine the mechanisms involved in the interactions between the energy source and herbage protein, both during fermentation and in the rumen.     

The effect of physical form of a silage on its voluntary consumption and digestibility by sheep

Six castrated male sheep were used in a two-period crossover experiment to investigate the effect of mincing on the voluntary intake and digestibility of a silage. The silage was a high-quality material, containing 68 % digestihle organic matter in the dry matter, prepared from S24 perennial ryegrass cut with a precision chop forage harvester and ensiled with the addition of 2.3 1 t^?1 formic acid as preser vative. The median size of particles in the unprocessed silage was 10–20 mm and in the minced silage 2–3 mm. The intakes of dry matter, organic matter and nitrogen for the minced silage were greater than for the unminced material, and for dry matter and organic matter intakes the differences were signi ficant (P<0.01). Mincing was associated, however, with a depression in the digestibility of all three constituents which offset the increase in intake. The intakes of digestible organic matter were 817 g d^-1 for the minced silage and 729 g d^?1 for the unminced material (P>0.05). Corresponding figures for digestible nitrogen intakes were 208 gd^?1and23.0 gd^?1 (P>005). It is concluded that with high-quality silage diets there is a physical component in the mechanism for the control of voluntary food intake. It is suggested that for sheep, depressions in the digestibility of dietary constituents are unlikely to occur until silages are chopped to a median particle size of 5–10 mm.     

The effect of stage of perennial ryegrass maturity at harvesting, fermentation characteristics and concentrate supplementation, on the quality and intake of grass silage by beef cattle

Abstract Two experiments were carried out in consecutive years to examine the influence of cutting date and restricting fermentation by carboxylic acid treatment on the nutrient intake from grass silage by beef cattle. In year 1, four cutting dates during July and August after a primary growth harvest and, in year 2, five cutting dates of primary growth between mid‐May and early July were examined. Herbage was ensiled either untreated or treated with high levels of acid additive (‘Maxgrass’, mean 8·6 l t^?1). Ninety‐six (year 1) or forty‐eight (year 2) continental cross steers were used in partially balanced changeover design experiments with each silage type either unsupplemented or supplemented with 4·5 (year 1) or 5·5 (year 2) kg concentrates head^?1 d^?1. Silage digestibility declined significantly between initial and final harvest dates (P < 0·001), whereas silage dry‐matter (DM) and digestible energy (DE) intakes were significantly higher in the initial compared with final harvest dates in both years of the study (P < 0·01). Similarly, silage DM and DE intakes, and total DM intakes, of acid‐treated and unsupplemented silages were greater than those of untreated and concentrate supplemented silages, respectively (P < 0·001). The results indicate that earlier cutting dates, and addition of acid to herbage before ensiling, can increase silage DM intake by beef cattle.     

THE ESTIMATION OF FEED INTAKE BY SHEEP ON A SILAGE DIET

The results of nineteen digestibility trials with sheep on different silages have been reported. From these data and from the results of forty trials with sheep carried out by Watson (15) it has been shown that Lancaster's method of calculating feed intake (9) can be applied to silages when the crude protein content is within the range studied (9–24% of the dry matter). For silages in this category, no advantage has been found in taking the nitrogen content of the silage dry matter into account in calculating feed intake.
The digestibility of crude protein and Lancaster's'constant'have both been correlated with the crude protein content of the silage dry matter for the fifty-nine trials considered (r = 0.767 and r = 0.452 respectively). Although it has been found unnecessary to take the protein content of silage into account in calculating dry matter intake when feeds containing 9.24% crude protein in the dry matter are used, evidence is presented which indicates that this factor is of considerable importance when herbage of low protein content is fed. The equation, which has been derived from the silage data considered, has been found to be valid when compared with the results of trials with fresh herbage and well preserved hays of low protein content.
The results of this work indicate that Lancaster's method is a suitable one for estimating the feed intake of animals engaged in self feeding of silage, provided the silage has been well preserved.     

Characteristics of baled silage made from first and second harvests of wilted and severely wilted forages

First and second harvests of lucerne (Medicago sativa L.), perennial ryegrass (Lolium perenne L.) and a lucerne–perennial ryegrass mixture [80 or 144 g kg^?1 dry matter (DM) of ryegrass] at the first and second harvests were cut and conditioned, wilted to 500 or 700 g DM kg^?1 then baled and stretch‐wrapped for silage on the same dates. Lucerne bales were denser (411 kg m^?3) than bales of perennial ryegrass (331 kg m^?3) (P < 0·05). After an 8‐month storage period, silage made from high DM‐content forage had a higher concentration of neutral‐detergent fibre (NDF) and was less digestible than that made from low DM‐content forage. Daily DM intakes by beef steers, when the silages of the second harvest were fed ad libitum, were 31·2, 31·2 and 22·3 g kg^?1 live weight for lucerne, lucerne–perennial ryegrass mixture and perennial ryegrass silages, respectively (P < 0·01), when the herbage had been wilted to 500 g kg^?1. In vivo digestibility of NDF in the lucerne–perennial ryegrass mixture silage (0·587) was significantly lower than that of perennial ryegrass silage (0·763) but higher than lucerne silage (0·518). Higher intakes of baled lucerne silage tended to offset its lower digestibility values. Lucerne–perennial ryegrass mixture silage had a higher DM and NDF digestibility than lucerne silage, indicating perhaps the presence of associative effects.     

THE USE OF PROPIONIC ACID AS AN ADDITIVE FOR MAIZE SILAGE

Two clamps of maize silage were prepared in mid-October, one with 0·33% propionic acid applied during harvesting and one without additive. The treated silage had a peak temperature 3°C below that of the untreated silage. The estimated loss of DM during ensiling was 8% greater in the untreated silage. The stability and acceptability of the silages were tested after exposure to air. The treated silage was slightly more stable than the untreated milage and the intakes by heifers were sligbtly greater. The digestibilities of the two silages estimated in wether sheep were similar, averaging 68·6% digestible OM in the DM at maintenance level and 63·5% at a high level of feeding. The intakes and growth rates of calves and beifers oifered the two silages were also similar.     

SILAGE AND MILK PRODUCTION. A COMPARISON BETWEEN WILTED GRASS SILAGES MADE WITH AND WITHOUT FORMIC ACID

Fonr grass sUages made in May from the same herbage were compared io a 16-week feeding experiment with 12 Ayrshire cows. The silages were made from herhage wilted for either 1 or 2 days, and with or without ¹/₂ gal formic acid per ton of herhage. The silages treated with acid were, on average, 5°C cooler than the untreated silages, had lower pH values, higher lactic acid contents, slightly higher DM and crude protein contents, and higher contents of digestible organic matter in the DM. The silages were fed ad lib. with a supplement of harley and groundnut cake. The intakes of silage DM were higher with the acid-treated than with the nntreated silage. The mean daily milk yields on the silages made with and without the acid additive were 35.4 and 33.1 lb (16.1 and 15.0 kg), respectively, for the silage, wilted for 1 day and 34.2 and 32.2 lb (15.5 and 14.6 kg) for the silage wilted for 2 days. The effects of the treatments on the composition of the milk were small. It is concluded that the silages treated with formic acid additive were superior to the nntreated silages as a feed for dairy cows.     

SILAGE AND MILK PRODUCTION, A COMPARISON BETWEEN GRASS SILAGES MADE WITH AND WITHOUT FORMIC ACID

Two grass sUages made in June and two made in September, all from the same field, were compared in a 16-week winter-feeding experiment with 12 Ayrshire cows. The silages were fed ad lib. with a supplement of barley and groundnut eake. One of the sUages made in June and one made in September had been treated with 1/2 gallon of formie acid per ton of herbage when cut, whereas the other two silages were untreated. The contents of digestible organic matter in the silage DM made with and without the acid additive were 67.4 and 63.8%, respectively, in the June silage, and 66-1 and 62.7% in the September silage. The intakes of silage and total DM were higher in the treatments containing the acid-treated silages than in those containing the untreated silages. The mean daily milk yields from cows fed on the sUages made with and without the additive were 36.3 and 33.8 lb (16.5 and 15.3 kg), respectively, for silages made in June, and 35.4 and 34.1 lb (16.0 and 15.5 kg) for those made in September. The solids-not-fat (S.N.F.) contents of the milk averaged 8.60 and 8.50% respectively, from the silages with and without the additive. It is concluded that the silage with the formic-acid additive was superior to the untreated silage as a feed for dairy cows.     

The nutritional value of pressed potato vine silage

Potato (Solanum tuberoswn L.) vines were studied to determine if they could be successfully ensiled and fed to ruminant animals. Russet Burbank potato vine and alfalfa (Medicago sativa L.) silages were fed to goats (Capra hircus) to measure in vivo digestibility and to determine if potato glycoalkaloids were aversive or toxic. No differences in the feeding patterns between control and experimental animals were observed. Alfalfa silage was more digestible than the Russet Burbank potato vine silage due to a higher content of digestible cell wall materials. Digestibility differences between potato clones and harvest dates were found when in vivo digestibility tests were performed. W729R was the most digestible clone studied, especially for the early harvest dates. Most of the clones had vines that were more digestible, in vitro, than alfalfa. Vine glycoalkaloid contents of the experimental clones were similar to the values reported in the literature. There was no apparent correlation between vine glycoalkaloid content and percent digestible dry matter. A positive correlation of 0.38 between digestible dry matter and dry vine yield was found. Potato vine silage, produced after pressing the vines to remove water, was an acceptable feed for ruminant animals. Additional in vivo testing is necessary to determine if toxic effects due to glycoalkaloids or pesticides from long term feeding will be a problem.     

Grass silage as a basic feed for store lambs. 1. Effect of wilting, chop length and stage of maturity of grass silage on intake and performance of store lambs

Two experiments were carried out with grass silages cut at a leafy (Experiment 1) and a more mature (Experiment 2) stage of growth to evaluate the effect of wilting and chop length on silage intake and performance of store lambs. In each experiment, the herbage was cut with a rotary mower and was either ensiled within 24 h as unwilled silage (U) or wilted for 1–3 d (W). Each silage type was harvested with either a double-chop harvester (D) or a precision-chop harvester (P). All silages were treated with formic acid at 3 1 t^?1 and were well preserved. The silages were fed ad libitum to Suffolk crossbred store lambs (twenty-four lambs per treatment) without any supplement over a period of 8 or 9 weeks. Wilting of the silages had little effect on silage intake (797 vs. 809g dry matter (DM) d^?1) or on lamb performance in Experiment 1. In Experiment 2, wilting of the D silage increased silage DM intake (589 vs. 534 g DM d^?1; +10%) and reduced the extent of liveweight losses. Wilting of the P silage reduced silage intake (770 vs. 791g DM d^?1; -3%) and reduced liveweight gains. In Experiment 1 intakes of the D silages were 650–667g DM d^?1 and just maintained lamb live weights. Intakes of the P silages were 39–49% higher than the D silages (927–968 g DM d^?1) and increased liveweight gains. In Experiment 2 intakes of the D silages were 534–589 g DM d^?1 and resulted in a loss in lamb live weight. Precision-chopping increased silage intakes by 31–48% (770–791 DM d^?1)in Experiment 2 and improved lamb liveweight gains. Lamb performance was higher on the UP silage than on the WP silage. The rumen retention lime (RRT), estimated from the rumen contents of the lambs at slaughter and their silage intake before slaughter, was much shorter for the lambs fed on the P silages (12.6–20.6 h) than those fed on the D silages (21.4–29.3 h) in each experiment. Silage intake and liveweight gain were positively related to silage in vivo DM digestibility (DMD), whereas RRT was negatively related to DMD. However, there were distinct differences between the P and D silages in the elevation and, to a lesser extent, in the slope of the regression lines, indicating that intake of D silage was limited by factors other than the digestibility of the silage The results of this study show that the chop length of grass silage had a far greater effect on intake and on lamb performance than silage digestibility, whereas wilting had little or no effect.     

Effects on milk production of feeding silage and sugar-beet pulp as separate components, in a mixed ration or as an ensiled blend

Twenty early-lactation British Friesian dairy cows were used in a five-treatment, partially balanced change-over design experiment, consisting of four periods each of 4 weeks' duration. Three treatments consisted of offering ensiled blends of silage and sugar-beet pulp produced by mixing 40 (S40), 80 (S80) and 120 (S120)kg beet pulp t^?1 herbage at ensiling. In two further treatments, an untreated silage was supplemented with 5 kg of beet pulp daily, either mixed with the silage prior to feeding (SM) or offered in two equal feeds each day (ST). Silage was offered ad libitum in all treatments, while in addition all cows received 1 kg d^?1 of a concentrate supplement containing 470 g crude protein kg^?1 fresh weight. Dry-matter intakes and milk fat + protein yields increased with increasing level of beet pulp inclusion in the diet, irrespective of whether it was offered in the form of an ensiled blend or as a supplement to an untreated silage. With the exception of D-value (digestible organic matter in the dry matter), which was significantly higher with treatments SM and ST than with the ensiled blend treatment (S80), no significant differences were identified in total ration digestibility or in the efficiency of nitrogen or energy utilization between methods of beet pulp inclusion in the diet. However, offering beet pulp in the form of an ensiled blend reduced the acetate/propionate ratio in rumen fluid compared with offering best pulp as a supplement to an untreated silage. When account is taken of differences in the efficiency of recovery of edible silage dry matter between ensilage systems and of differences in dry-matter intakes between treatments, total milk fat + protein outputs per 10000 kg herbage dry matter ensiled were 55 (s.e. 50·9) and 78 (s.e. 47·5) kg lower when equal quantities of beet pulp were offered as part of an ensiled blend rather than as a supplement to an untreated silage, as in treatments SM and ST respectively. However, these differences were non-significant. Alternatively, in order to produce an equal milk output from ensiling a given quantity of herbage, 12·5 and 16·2% more beet pulp would have been required if the beet pulp had been mixed with the herbage at ensiling, rather than offered as a supplement, as in treatments SM and ST respectively.     

Effect of herbage water-soluble carbohydrate content and weather conditions at ensilage on the fermentation of grass silages made on commercial farms

A total of 1009 samples of silage made in bunker silos on commercial farms between 1972 and 1978 was analysed to investigate the effect of herbage water-soluble carbohydrate content (WSC) and weather conditions at ensilage on fermentation as measured by ammonia-N concentration and pH of first-cut grass silages.
Silage dry matter (DM) content had the major effect on fermentation. Factors influencing silage DM were rainfall and hours of sunshine during silage making, and DM content of the grass cut. WSC content of herbage ensiled also had a significant effect on subsequent fermentation. The major influences on herbage WSC were hours of sunshine and rainfall during the growing season.
The effect of chemical additives, albeit at poorly defined and often inadequate rates, was small in comparison to that of silage DM.
The minimum DM necessary to produce well-fermented silage without additive was approximately 260 g kg⁻¹. Use of formic acid significantly reduced this requirement to 240 g kg⁻¹ and to 252 g kg⁻¹ for sulphuric add + formalin. The results indicate that the minimum herbage WSC necessary to prevent a clostridial fermentation developing in silage with a DM content of 230 g kg⁻¹ is approximately 37 g kg⁻¹ without additive and 30 g kg⁻¹ with formic acid.
It is concluded that on commercial farms, weather conditions i.e. amount of rainfall and sunshine prior to and at ensilage, have a greater effect on subsequent silage fermentation than additive use.     

Effect of formic acid and a bacterial inoculant on the amino acid composition of grass silage and on animal performance

An Italian ryegrass and hybrid ryegrass sward was harvested on 11 May 1994. The mean dry‐matter (DM) content of the herbage was 197 g kg^–1 fresh matter (FM), and mean nitrogen and water‐soluble carbohydrate contents were 20 and 272 g kg^–1 DM respectively. Approximately 72% of total nitrogen (TN) was in the form of protein‐nitrogen. The herbage was treated with either no additive, formic acid (3·3 l t^–1) (Add‐F, BP) or inoculant (2·3 l t^–1) (Live‐system, Genus) and ensiled in 100 t silos. Changes in effluent composition with time showed that silage fermentation and protein breakdown were delayed by treatment with formic acid. Formic acid and inoculant treatments also inhibited amino acid catabolism during ensilage. All silages were well fermented at opening with pH values < 4·0 and ammonia‐N concentrations of ≤ 50 g kg^–1 TN after 120 d ensilage. Treatment had an effect on protein breakdown as measured by free amino acid concentration, with values of 21·5, 18·2 and 13·2 mol kg^–1 N at opening (191 d) for untreated, formic acid‐treated and inoculated silages respectively. Amino acid catabolism occurred to the greatest extent in untreated silages with significant decreases in glutamic acid, lysine and arginine, and increases in gamma amino butyric acid and ornithine. The silages were offered ad libitum without concentrate supplementation to thirty‐six Charolais beef steers for a period of 69 d (mean live weight 401 kg). Silage dry‐matter intakes and liveweight gains were significantly (P < 0·05) higher on the treated silages. Silage dry‐matter intakes were 7·42, 8·41 and 8·23 kg d^–1 (s.e.d. 0·27) with liveweight gains of 0·66, 0·94 and 0·89 kg d^–1 (s.e.d. 0·058) for untreated, formic acid‐treated and inoculated silage‐fed cattle respectively. In conclusion, additives increased the intake of silage and liveweight gain by the beef steers, and it is suggested that this may be caused in part by the amino acid balance in these silages.     

A COMPARISON OF SILAGE MADE IN POLYETHYLENE CONTAINERS WITH AND WITHOUT THE REMOVAL OF AIR BY EVACUATION

The effect of partial evacuation of air from polyethylene containers, at the time of sealing, on the chemical composition and nutritive value of silage was determined. Four containers of 500-kg capacity were filled with herbage harvested from each of four swards, namely, S170 tall fescue, Syn I tall fescue, Tetila Tetrone Italian ryegrass and an S24 perennial ryegrass/S100 white-clover mixture. All the containers were sealed immediately after filling and half of them were evacuated. The silages resulting from these treatments were offered ad lib. to store sheep and the intake and digestibility of the dry matter determined. The evacuation of the silos had no significant effect upon the chemical composition of the silage produced. The intake and digestibility of the dry matter in the silages were not significantly influenced by evacuation.     

The effects of cereal supplementation of grass and maize silages

Grass and maize silages were fed alone and with two levels of a barley supplement to Friesian steers of about 300 kg liveweight. The organic matter (OM) intakes of grass and maize silage were similar although the OM digestibilities of the silages were 0·722 and 0·649 respectively. For both silages, barley supplementation increased total OM intake and total digestible OM intake by 0·46·0·08 and 0·37·0·06 g per g OM of supplement respectively. Liveweight gains on grass and maize silage diets were 1·38 and 1·20 kg d^-1 respectively, but the differences were not significant (P> 0·05).     

RED CLOVER SILAGE FOR MILK PRODUCTION

Nine wilted silages made from tetraploid red clover were fed in four winter-feeding experiments to 32 Ayrshire cows. The DM content of the silages averaged 23±5% (range 15±8–27±1) and contained an average of 19±6% crude protein, 14±2% digestible crude protein and 52±0% digestible organic matter. The pH values varied from 4±0–6±2, depending on the DM content and mechanical treatment of the crop, and the rate of application of formic add. The silages were fed ad lib.with supplementary concentrates and the average daily intakes of silage DM ranged from 16±0–24±0 Ib/cow (7±3–10±9 kg). The highest daily intakes were obtained with double-chopped silage containing 24–26% DM with a pH of 4±0. Although the red-clover silage had a high content of N and apparently provided a balanced ration with the addition of barley, yields of milk were increased significantly by substituting 1 Ib (0±4 kg) groundnut cake for 1 Ib barley in the daily ration of the cow. The silage contained oestrogenic compounds but the breeding pattern and fertility of the cows were not affected adversely. The tetraploid red clover produced approximately 9000 Ib DM/ac (10 100 kg/ha) in the 1st-harvest year and it is concluded that a safe and highly palatable silage with high intake characteristics can be made if the clover is wilted to about 24% DM, double chopped, and formic acid is applied at the rate of 0±5 gal/ton.     

THE EFFECT OF THE ADDITION OF ARACHIS OIL ON THE CONSERVATION OF HEAVILY–WILTED HERBAGE SEALED IN POLYTHENE FILM

A conservation experiment is described in which arachis oil was used to improve the energy content of heavily-wilted herbage of approximately 46% dry matter. The 2 oil-treated herbages and 2 control silages were enclosed in polythene film. The DM losses from all 4 silos were high compared with losses normally encountered in completely sealed silos. This is attributed to secondary fermentation which occurred after the silos were opened. The oil had a depressing effect on the digestibility of the mineral matter of both grass and silage. The metabolizable energy values of the silages are reported and discussed.     

Voluntary intake,digestibility and nitrogen utilization by sheep fed ensiled forage legumes

An experiment was conducted to compare the nutritive value of a range of ensiled forage legumes. Silages were prepared from late second‐cut lotus (Lotus corniculatus), first‐cut sainfoin (Onobrychis viciifolia) and both early and late second‐cut red clover (Trifolium pratense) and lucerne (Medicago sativa). Each experimental silage was offered to six Suffolk‐cross wether lambs, aged 10 months, housed in metabolism crates. Voluntary intakes of dry matter ranged from 71 to 81 g kg^?1 liveweight^0·75 d^?1. Voluntary intakes were similar on the lotus, sainfoin and late‐cut red clover silages, but the voluntary intake on the lotus silage was significantly higher than that on the lucerne silages and early‐cut red clover silage. Digestibility of organic matter in the dry matter was highest for the lotus silage (0·650), and lowest for the sainfoin silage (0·527). Although most of the N in the sainfoin silage appeared to be in an indigestible form, N digestibility was approximately 0·70 for the other legume silages. The highest loss of N in urine, 0·75 of N intake, was recorded for lambs offered the lucerne silage. Differences in N intake, N loss in faeces and N loss in urine led to statistically significant differences in the amount of N retained, with the highest and lowest N balances recorded for the lotus (16 g N d^?1) and sainfoin (?2 g N d^?1) silages respectively. The results confirm that these high protein forages have high intake potential. While low N digestibility appears to limit the nutritional value of sainfoin, further research could formulate feeding strategies that improve the efficiency with which the protein from red clover, lucerne and lotus is utilized.     

SILAGE AND MILK PRODUCTION, A COMPARISON BETWEEN WILTED AND UNWILTED GRASS SILAGES MADE WITH AND WITHOUT FORMIC ACID

Three grass silages, all made in mid-Jnne from the same herbage, were compared in a 12-week winter-feeding experiment with 12 Ayrshire cows. The silages were fed ad lib. with a supplement of moist barley and gronndnut cake. Silage A was made from unwilted berbage with no additive, silage B from identical berbage treated with 1/2 gal of formic acidJton (2.24 L/tonne) of berbage, and silage C from the herbage after wilting for 28 b. the DM contents of silages A, B and C were 20.5, 21.1 and 31.8%, respectively, and the contents of digestible organic matter in the DM were 58.9, 68.8 and 67.8%. Silage and total DM intake were bigbest on the treatment containing silage B. the mean dafly milk yields on the treatments with sflages A, B and C were 35.1, 38.0 and 34.3 Ib (15.9, 17.2 and 15.6 kg), respectively and the solids-not-fat (S.N.F.) contents of the milk averaged 8.53, 8.54 and 8.50%. It is concluded tbat silage B, the unwilted silage with formic-acid additive, was superior to botb the untreated and the wilted silage as a feed for dairy cows.