Relative differences in sugar content of basal and apical portions of Russet Burbank potatoes

Basal and apical portions of mature and immature Russet Burbank tubers stored at 42 F (5.6 C), 45 F (7.2 C), and 48 F (8.0 C) were analyzed for sucrose and reducing sugars at seven monthly intervals for three seasons. Percent relative difference of reducing sugars (between mature and immature tubers) was greater in the apical than the basal portion. On the other hand, seasonal differences in percent reducing sugars were reflected more in the basal portion by greater relative differences among seasons than in the apical portion. Statistical analysis showed no trend or influence of maturity or storage temperature in affecting the relativeratio of reducing sugars between the basal and apical portions. Differences among growing seasons appeared to affect this ratio. Tubers from the 1970–71 season had more equal relative amounts of sugars in the two portions than did the tubers from 1968–69 or 1969–70 seasons. Ratio ofsucrose between the basal and apical portions was not affected appreciably by maturity, storage temperature or season, although immaturity and lower storage temperatures caused slightly greater overall sucrose accumulation. Sucrose and reducing sugar contents were poorly correlated regardless of maturity, storage temperature or different seasons.     

Starch-sugar interconversion inSolanum tuberosum L. II. Influence of membrane permeability and fluidity

A strong relationship was found between membrane permeability and starch to sugar conversion in stored potatoes. Tissue from tubers stored at low temperature had higher sugar content and more permeable membranes than from that of tubers stored at high temperature. Tubers, moisture stressed during growth and stored at 5.5 C, accumulated more reducing sugars and had more permeable membranes than tubers from normally irrigated plants when stored at the same temperature. The basal portion of Russet Burbank tubers accumulated more sugars and was also found to have significantly more permeable membranes than the apical portion. Examination of membrane order with spin-labeled lipid probes showed that the order parameter of amyloplast membranes was less at low temperatures than when measured at higher temperatures. Membranes examined with spin-labeled lipid probes from tubers stored at 5.5 C and 15.5 C showed similar order parameters at all temperatures measured between 0 and 25 C with a linear decrease in S values at higher temperatures. These results strongly indicate a physical change of membranes with changing temperature, thus influencing sugar accumulation, which is also reversible, as indicated by loss of sugars during the reconditioning process.     

Growth and preharvest stress and processing quality of potatoes

The quality factors influenced by stress conditions are: total dry matter or starch content and distribution of starch, sugar content, types of sugars and distribution, texture, mealiness, flesh color, tuber size and shape, and tuber defects such as growth cracks, and hollow heart. Early stress during growth can cause tubers to become pointed on the stem-end with low starch content and high reducing sugars. Uneven growth due to stress results in growth cracks, or hollow heart and malformed tubers. Varieties differ considerably as to susceptibility. Stress generally causes lower starch content in tubers which results in greater sugar accumulation in storage. Relative maturity is also influenced by stress during growth. Immature and over-mature tubers (from prematurely dead plants) accumulate greater amounts of sugar in storage than tubers properly matured. Stress can also cause poor texture of processed products, such as tough texture of French fries as well as lack of mealiness. Flavor and color of processed products can also be detrimentally influenced by stress.     

The development of translucent end tubers

A study was made to gain greater understanding of the development of translucent end tubers which caused problems for french fry processors duringthe processing season of 1971–72. Analysis of seven commercial and three experimental samples revealed a lower dry matter and higher reducing sugar content in the basal portion of the tubers. Normally Russet Burbank potatoes contain higher dry matter in the basal end. A study of dry matter changes during growth and development in 1971 and of moisture stressed potatoes in 1972 showed a reversal in dry matter content and an uneven distribution of reducing sugars, whereas the normally irrigated potatoes maintained a higher percentage of dry matter in the basal portion throughout. A decrease in pH of the tissue accompanied the loss of starch from the basal portion of moisture stressed potatoes. As postulated by other research workers, the loss of dry matter is attributed to initiation of active growth of the apical end, thereby utilizing carbohydrates from the basal end following a period of stress. Immaturity of tubers and storage at low temperatures immediately after harvest caused water stressed potatoes to develop increased numbers of translucent ends in storage.     

Starch to sugar interconversion inSolanum tuberosum L. I. Influence of inorganic ions

No relationship could be found between calcium, magnesium, potassium, total phosphorous contents, and reducing sugar accumulation in apical and basal portions of Russet Burbank tubers. However, a highly significant positive correlation was found between inorganic phosphorous content of tubers and reducing sugar accumulation. Inorganic phosphorous and sugar levels were highest in the basal portion of moisture stressed and low temperature stored tubers, suggesting a relationship between inorganic phosphorous and enzymatic activity in the interconversion of starch to sugars in potatoes. Tubers stored at 5.5 C and 15.5 C had higher contents of calcium and magnesium in the basal portion and significantly higher potassium content in the apical portion. Storage temperatures or moisture stress during growth appeared to have no significant influence on calcium, magnesium, potassium or total phosphorus contents of the tubers.     

Some factors influencing the pH of apical and basal portions of Russet Burbank and Norgold Russet potatoes

Studies were made of pH changes of apical and basal portions of Russet Burbank and Norgold Russet potatoes during growth and in storage. Russet Burbank had a higher pH of the apical portion during the later stages of growth as compared to the basal portion. No significant differences between the two portions were found during growth with Norgold Russet. After a period of moisture stress the pH of the basal portion of Russet Burbank dropped significantly. The basal portion of both varieties at higher specific gravities had higher pH after storage than the apical portion. A highly significant negative correlation was obtained after storage between reducing sugar levels of tubers of different specific gravities and pH levels in both varieties. There were no significant differences in pH among storage temperatures of 34, 38, 42 and 45 F. However, the pH level at 48 F storage was significantly higher than at the other temperatures. The pH level of tubers from seedlings and varieties were poorly correlated with their reducing sugar contents after storage. It appears that pH can be an indicator of potential chip color only within the confined limits of a specific lot.     

Respiration rates and sugar accumulation in normal and moisture stressed Russet Burbank potatoes

Studies were made on Russet Burbank tubers grown under normal and moisture stressed conditions. Stressing the tubers caused increased reducing sugar accumulation in the basal end during storage. Respiration rates of apical and basal portions of stressed and normal tubers were followed during the growing season and in storage. Although its sugar content was greater, the basal end of stressed tubers respired at a significally slower rate than apical ends from the same tubers. Normal basal and apical portions showed no statistical differences in respiration although the apical end respired at a slightly faster rate than the basal end. A positive linear correlation between sugar concentration and respiration rate of the apical portion was obtained. Differences were found in the abilities of apical and basal ends to alter respiration rates in response to changes in sugar concentration. The resprationof the apical end was the more sensitive to increased sugar concentration. The basal end of stressed tubers lost some of its ability to respond, probably because of increased physiological aging caused by the stress conditions.     

Factors influencing reconditioning of Russet Burbank potatoes

Studies were made to determine factors which influence reconditioning of Russet Burbank tubers. Tubers from plants grown under low fertility were more difficult to recondition than tubers from plants adequately fertilized. Low specific gravity potatoes were also more difficult to recondition than high specific gravity potatoes even though the initial sugar content was approximately equal. The stem portion of Russet Burbank tubers lost considerably less sugars than the bud portion during the reconditioning process. It appears that factors which are responsible for high sugar development are also responsible for difficulty in sugar loss. Results also indicate greater difficulty in reconditioning early in the storage period with the loss of sugars becoming easier as the storage season progressed into spring.     

Influence of moisture stress during growth on14CO2 fixation and translocation inSolanum tuberosum L.

Moisture stressing of potato plants resulted in reduced¹⁴CO₂ fixation and translocation of labelled photosynthates from leaves to tubers. A majority of the label was recovered in the sugar fraction of both leaves and tubers. The amount of labelled¹⁴C recovered in the organic acid fraction of tubers of normally irrigated plants was significantly higher than in tubers which had been moisture stressed. In the other fractions, the differences were not significantly different. Injection of uniformly labelled sucrose into the basal portion of attached tubers of stressed and non-stressed plants showed greater translocation of labelled carbon by tubers of stressed plants from basal to the apical portion and also into the stems as compared to non-stressed plants.     

Biochemical and molecular control of cold-induced sweetening in potatoes

The benefits of being able to process potatoes directly into chips or fries from cold storage (2 to 4 C) include less shrinkage, retention of dry matter, decreased disease loss, extended marketability, and the elimination of the need for dormancy-prolonging chemicals. Unfortunately at low temperature, potato tubers undergo a phenomenon known as cold-induced sweetening where the rate of conversion of starch to reducing sugars (i.e., glucose and fructose) is accelerated. As raw potatoes are sliced and cooked in oil at high temperature, the accumulated reducing sugars react with free amino acids in the potato cell forming unacceptably brown- to black-pigmented chips or fries via a non-enzymatic, Maillard-type reaction. Potatoes yielding these unacceptably colored products are generally rejected for purchase by the processing plant. All commercial potato cultivars presently used for the production of potato chips and fries accumulate excess free reducing sugars when exposed to cold stress. If a “cold-processing potato” was available, energy savings would be realized in potato-growing regions where outside storage temperatures are cool. In regions where outside temperatures are moderately high, increased refrigeration costs may occur. This expense would be offset, however, by removal of the need to purchase dormancy-prolonging chemicals, by a decreased need for disease control and by improvement of long-term tuber quality. The primary goal of this review is to describe recent research of a biochemical and molecular nature that relates to the underlaying mechanisms regulating post harvest, cold-induced sweetening in potato tubers. No attempt was made to outline the extensive research conducted on the genetic manipulation of carbon metabolism between starch and free sugars during photosynthesis and/or during potato development in relation to source/sink interactions.     

Preconditioning and storage of chemically immature Russet Burbank and Shepody potatoes

Russet Burbank and Shepody potatoes of different chemical maturities (i.e. varying sucrose concentrations) at harvest were preconditioned by holding at 15 C for various durations to lower the concentration of reducing sugars to levels acceptable for processing as french fries out of 8 C storage. In chemically immature tubers (i.e. those harvested with a high sucrose), sucrose declined after harvest and stabilized in storage regardless of the preconditioning period. However, there was a temporary small increase in sucrose after harvest in more chemically mature tubers. The concentrations of the reducing sugars glucose and fructose were low at harvest, usually < 1.0 mg g^?1, but increased rapidly during the first 30 to 45 d of storage in tubers with more than 2.0 mg g^?1 sucrose at harvest. Preconditioning for up to 70 d at 15 C either limited the increase in reducing sugars or lowered them more rapidly during storage than when preconditioned for only 14 d. Fresh weight loss of Russet Burbank and Shepody was greater in physically and chemically immature tubers compared to more mature tubers. Extended preconditioning of Russet Burbank and Shepody resulted in minimal additional weight loss.     

Changes in sugar content and invertase activity in tubers of some Indian potato varieties stored at low temperature

Summary Significant differences were found in sugar content and invertase activity of tubers of 8 Indian cultivars stored at 3–5°C. Freshly harvested tubers of all the cultivars had low amounts of sugars, which increased during storage, and little or no basal invertase activity but both basal and total invertase activities in all the cultivars increased during storage. There were significant correlations between the reducing sugar content and basal and total invertase activities. Publication no. 116, Central Potato Research Institute, Shimla.     

Storage temperature and maleic hydrazide effects on sprouting,sugars, and fry color of Shepody potatoes

The effect of low storage temperature (4 to 8 C) and maleic hydrazide (MH) treatment on sprouting, sucrose and reducing sugar (glucose and fructose) concentrations in tubers, and fry color-sugar relationships of Shepody were determined and compared to Russet Burbank. Sprouting of Shepody was delayed by 3 to 15 weeks when the temperature was lowered from 8 to 6 or 5 C, respectively. Shepody accumulated more sugars at all storage temperatures than Russet Burbank. The reducing sugars accumulated in Shepody at 5 and 6 C could be lowered to levels acceptable for processing for about 24 weeks after harvest by reconditioning at 18 C for at least two weeks. MH increased glucose in Shepody stored at 5 C compared to untreated tubers. Excessive sprouting (> 10 cm sprout length) of nonsprout inhibited Shepody occured late in storage with four weeks reconditioning. Although not statistically significant, MH tended to cause darker fry color per unit of reducing sugar in Shepody compared to tubers from untreated plants. Storage of Shepody tubers not treated with MH could be extended a few weeks by temperatures < 8 C only if there is precise control over temperature, sugars are closely monitored, and reconditioning is rapid to minimize sprouting.     

Changes in ascorbic acid content during growth and short-term storage of potato tubers (Solanum tuberosum L.)

Changes in ascorbic acid levels were followed in apical and basal portions of Russet Burbank and Kennebec tubers during growth and development. Influence of nitrogen fertilization during growth and short-term storage on ascorbic acid level of the tuber was also studied. Changes in ascorbic acid content during growth showed two distinct phases, the first phase being characterized by an increase in ascorbic acid content with growth and development of the tuber, followed by a second phase which showed a decrease in ascorbic acid content with increasing maturity. The shift from the first phase to the second phase occurred earlier for Kennebec than for Russet Burbank. Increased nitrogen fertilization resulted in a delay of this shift from the first to the second phase. The apical portion of the tuber consistently showed higher ascorbic acid content than the basal portion. Kennebec tubers were higher in vitamin C content than tubers of Russet Burbank cultivar. High nitrogen fertilization resulted in less ascorbic acid content during growth. Storage of tubers for four weeks at 5.5°C also resulted in a marked decrease in ascorbic acid content of tubers. Nitrogen fertilizer rate during growth had no influence on the decrease in ascorbic acid content of tubers in storage.     

Effects of short periods of chilling and warming on potato sugar content and chipping quality

Two days of chilling (exposure to 1 C) had no detectable effect on chip color or sugar content of potato (Solanum tuberosum L.) tubers. Four days of chilling led to increases in sugars if potatoes were stored at 10 C or 19 C for 3 to 4 days after chilling ended. Longer chilling gave increases in sugars by the end of the chilling period, and returning tubers to 10 C or 19 C magnified the response. Glucose and fructose gave similar increases from chilling treatments, except that glucose levels were consistently greater. Sucrose was increased by chilling, but the response pattern did not always resemble that of the reducing sugars. Twenty-seven days of storage at 19 C after chilling lowered levels of reducing sugars late in the storage season but not in December. Cultivars differed in response to chilling. Kennebec tubers accumulated far more reducing sugars but much less sucrose than did Norchip tubers. Storage at 19 C after chilling lowered the sucrose content of Kennebec and Monona while raising that of Norchip.     

Irrigation and soil temperature effects on Russet Burbank quality

Potato growers in the Pacific Northwest suffer large economic losses in seasons with above normal temperatures, due to excess reducing sugars in tubers, which cause dark-end French fries. Our objective was to study irrigation management effects on potato quality, particularly the factors causing dark-end French fries or sugar-end syndrome. Solid-set sprinkler irrigated plots were established on potatoes at Kimberly, Idaho during the 1987, 1988 and 1989 irrigation seasons, and at Parma, Idaho in 1987 and 1988. Irrigation treatments were high and low frequency (3/week and I/week) and two or three water application amounts referenced to estimated evapotranspiration (ET, ET+20%, and ET-20%). Neither frequency nor amount significantly affected yields. Irrigation frequency had more influence on potato quality than application amount. More frequent irrigation produced slightly higher quality tubers and lower incidence of dark-ends when fried. Soil temperature was inversely related to tuber grade quality and directly related to percentage of sugar-end tubers. Soil temperature was about 0.5 C lower under the high frequency than under the low frequency irrigation regime. On these silt loam soils, allowing available soil water to decline to 50 percent had no adverse affect on yield or quality.     

Suitability of Organic Potatoes for Industrial Processing: Effect of Agronomical Measures on Selected Quality Parameters at Harvest and after Storage

Three factorial field experiments were conducted in two consecutive years (2003 and 2004) on two sites in order to examine the impact of preceding crop, pre-sprouting, N and K fertilization and cultivar on quality attributes of organically grown potatoes destined for processing into French fries or crisps. Tuber dry matter (DM) concentration, glucose and fructose concentrations, as well as the colour of crisps and the quality score of French fries, were assessed at harvest and after a 4-month storage period. Results suggest that tubers from organic potato cropping may be expected to have sufficiently high tuber DM concentrations (>19%) for processing into French fries without impairing the texture of the fries when concentrations exceed 23%. DM concentrations of tubers for crips (cv. Marlen) fell short of the required minimum of 22% when a combined N and K fertilizer was applied. The tuber DM concentration was significantly lower following peas than following a legume-grass/clover ley or cereals (oats or winter wheat), but only in one of two seasons. Pre-sprouting increased tuber DM concentration considerably, especially in the growing season with a high incidence of Phytophthora infestans (+1.2% absolute increase). Tuber DM concentration was significantly higher after storage in two of three experiments (+0.4 and 0.5% absolute increase). Cultivars belonging to the very early and early maturity type showed the largest relative increase in reducing sugars concentrations due to storage, ranging between 300 and 1,100%. The medium-early cv. Agria and medium-late cv. Marena proved to be best suited for processing into French fries under conditions of organic farming, as only minor deviations from the highest quality standards were established at harvest (quality index at 4.3 and 4.1, respectively). A consistently high crisp quality was achieved by the medium-early cv. Marlen, with L-values of 70.8 and 66.7 at harvest and after storage, respectively. Overall, results show that the quality variables were mainly affected by cultivar, season, storage and their interaction. The effect of agronomical measures, such as fertilization, preceding crop and pre-sprouting of seed-tubers, was rather small and their effect on internal tuber quality and quality of fried products may hardly be predictable. The quality standards for tuber raw stock can be accomplished best when adequate cultivars are chosen.     

Sucrose accumulation during high-temperature storage of potato tubers

Summary Potato tubers stored in a farm store from April to October (monthly average temperature between 24.7 and 36.2°C) accumulated considerable quantities of total sugars. Sucrose was the main component though in the variety Kufri Chandramukhi reducing sugars accumulated in amounts equal to sucrose.     

Relationship of specific gravity to sugar accumulation in stored Norgold and Russet Burbank potatoes

Studies were made on the influence of growing season and low fertility on reducing sugar accumulation in storage of the stem and bud portions of Russet Burbank and Norgold Russet potatoes separated into different specific gravity groups. The stem portion of Russet Burbank had a significantly different intercept and slope of regression line (between specific gravity and reducing sugar accumulation) than the bud portions of the same tubers indicating higher reducing sugar accumulation in the stem portion as well as differences in behavior as to amount accumulated at the different specific gravity levels. No significant difference in sugar content or behavior at the different specific gravity levels was obtained between stem and bud portions of Norgold Russet. Growing season did not cause significant differences in total sugar content or slope of the regression lines within the stem or bud portions of Russet Burbank. Low fertility level resulted in significantly higher sugar accumulation in the stem portion of Russet Burbank as compared to adequate fertility and there was also a trend toward less influence of specific gravity on sugar accumulation at the lower fertility level. Low specific gravity Russet Burbank potatoes tended to be more variable in sugar accumulation from year to year and also had wider differences in sugar accumulation between stem and bud portions than high specific gravity potatoes.     

Changes in malic and citric acid contents during growth and storage ofSolanum tuberosum L.

Changes in malic and citric acid contents of apical and basal portions of tubers were followed during growth and development and in storage. Malic acid content was highest in the early harvested potatoes and decreased as the growing season progressed until a minimum level was reached approximately the first to the middle of September, and then started to increase again as temperatures got cooler in the fall. Malic acid content closely paralleled sucrose content during growth and development. The period at which low malic acid content was observed coincided with optimum time of harvest. Citric acid content did not show a definite pattern of behavior with increase in maturity. Low temperature storage resulted in decreased pH and an increase in malic and citric acid contents of tubers. Both acid contents were higher in the apical portion, although the pH was lower in the basal portion. No significant correlation was found between pH and content of either acid.