章古台沙地3种不同针叶树种天然更新对比

通过对章古台沙地樟子松、赤松、油松等3种不同针叶树种天然更新对比试验,结果表明,3种针叶树1年生天然更新强度分别为3 594～5 133、20 300～50 000和8 300～13 900株.hm-2;赤松天然更新的强度好于油松,油松好于樟子松;樟子松天然更新1年生幼苗较难越冬,经覆盖后幼苗越冬成活率达66%;赤松、油松1年生幼苗相对较易越冬,越冬成活率分别为17.20%和20%,经覆盖后幼苗越冬成活率分别为54.94%和51.28%。因此,应采取人工促进措施进行樟子松、赤松、油松天然更新。     

辽西北沙地5种针叶树光合特征比较

2015年,在辽宁省彰武县应用Li-6400光合仪,对樟子松(Pinus sylvestris var.mongolica Litv.)、彰武松(Pinus densiflora var.zhanguensis)、油松(Pinus tabulaeformis Carr.)、赤松(Pinus densiflora Sieb.et Zucc.)和长白松(Pinus sylvestriformis)光合作用的日变化进行观测。结果表明:5种针叶树在8月光合速率的日变化趋势基本相同,均表现为双峰型,并有明显的光合"午休"现象,其中彰武松光合速率最高、长白松光合速率较低。通过光响应曲线的分析得出:在0~2 000μmol m~(-2)s~(-1)光强下,光合能力顺序为长白松赤松樟子松油松彰武松;赤松和樟子松的光补偿点较高,为110μmol m~(-2)s~(-1);樟子松的暗呼吸速率最大,为2.338μmol m~(-2)s~(-1)。     

赤松天然更新规律及人工促进更新技术的研究

本文对赤松天然更新的规律进行了调查，并对人工促进更新技术进行了研究。结果表明，赤松初果期为１２ａ，盛果期为５１ａ，公最大更新幅度为４０ｍ；更新幼苗幼树的７０％以上集中分布在距母树９ｍ或树高１．２倍以内。人工促进效果以拖拉机耙和人工耧地好；只要耧掉草皮，移栽７－８ａ幼树，３－４ａ就可达到更新指标；单位面积上培育母树的数量随时间长而减少。     

Preformation in vegetative buds of Prunus persica: factors influencing number of leaf primordia in overwintering buds

We investigated the influence of bud position, cultivar, tree age, tree carbohydrate status, sampling date, drought and light exposure on the number of leaf primordia formed in dormant vegetative peach buds (Prunus persica (L.) Batsch) relative to the number of primordia formed after bud break (neoformed). During winter dormancy, vegetative peach buds from California and Italy were dissected and the number of leaf primordia recorded. Between leaf drop and bud break, the number of leaf primordia doubled from about five to about 10. Parent shoot length, number of nodes on the parent shoot, cross-sectional area of the parent shoot, bud position along the parent shoot and bud cross-sectional area were correlated with the number of leaf primordia. Previous season light exposure, drought and tree carbohydrate status did not affect the number of leaf primordia present. The number of leaf primordia differed significantly among peach varieties and tree ages at leaf drop, but not at bud break. Our results indicate that neoformation accounted for all shoot growth beyond about 10 nodes. The predominance of neoformed shoot growth in peach allows this species great plasticity in its response to current-season conditions.     

Terminal bud failure of black cottonwood (Populus trichocarpa) exposed to salt-laden winter storms

At coastal sites, trees are exposed to marine aerosols that may cause foliar necrosis and shoot dieback, which can result in deformed crowns and contorted stems. A six-year study of leaf primordia in terminal buds of black cottonwood trees (Populus trichocarpa Torr. & Gray) on Heimaey Island off the south coast of Iceland was undertaken to elucidate the physiological events associated with salt-deposition-related bud failure. Leaf and bud lengths, dry mass, water content and chloride concentrations were monitored and related to four phenological stages: (1) bud set; (2) dormancy induction; (3) dormancy release; and (4) bud break. The trees set buds in July and shed their leaves by late September. Leaf primordia generally stopped growing by September 10 +/- 22 days and attained midwinter water content in late September. Leaf growth commenced in the terminal buds by March 2 +/- 16 days, but mean dates of bud swelling and bud break were April 29 +/- 19 and May 10 +/- 12 days. In summer and until November, chloride concentrations in leaf primordia were low, but increasing. Chloride concentrations remained stable from December to February, even though the dormant trees were exposed to large amounts of marine aerosols. In February and March, three events occurred more or less simultaneously: (1) leaf extension growth commenced; (2) chloride concentration surged in the leaf primordia; and (3) the leaf primordia began to hydrate. Following dormancy release, growth and hydration of leaf primordia were negatively related to chloride concentration in the leaf primordia, with inhibition of leaf growth, tissue hydration and chloride acquisition occurring at a chloride concentration threshold estimated at 7.3 mg Cl- g(-1) tissue water. Necrosis of leaf primordia was observed above 14 mg Cl- g(-1) tissue water. Growth and hydration of leaves at bud break in mid-May was explained by a three-parameter logistic model of chloride concentration in leaf primordia at the end of March. By mid-May, 90% of all buds remained non-necrotic, but only 56% the terminal buds had broken. Salt alone explained the observed growth suppression of leaf primordia in the buds and the resultant failure of terminal buds to break by mid-May.     

Optical properties of bud scales and protochlorophyll(ide) forms in leaf primordia of closed and opened buds

The transmission spectra of bud scales of 14 woody species and the 77 K fluorescence emission spectra of the innermost leaf primordia of closed and opened buds of 37 woody species were studied. Pigment concentrations were determined in some species. Bud scales had low transmittance between 400 and 680 nm with a local minimum around 680 nm. Transmittance increased steeply above 680 nm and was > 80% in the 700-800 nm spectral region. Significant protochlorophyllide (Pchlide) accumulation was observed in leaf primordia of tightly packed, closed buds with relatively thick, dark bud scales. In common ash (Fraxinus excelsior L.) and Hungarian ash (Fraxinus angustifolia Vahl.), the innermost leaf primordia of the closed buds contained protochlorophyll (Pchl) and Pchlide (abbreviated as Pchl(ide)), but no chlorophyll. We observed Pchl(ide) forms with emission maxima at 633, 643 and 655 nm in these leaves. Complete transformation of Pchlide(655) (protochlorophyllide form with maximum emission at 655 nm) into Chlide(692) (chlorophyllide form with maximum emission at 692 nm) occurred after irradiation for 10 s. The innermost leaf primordia of the buds of four species (flowering ash (Fraxinus ornus L.), horse chestnut (Aesculus hippocastanum L.), tree of heaven (Ailanthus altissima P. Mill.) and common walnut (Juglans regia L.)) contained Pchl(ide)(633), Pchl(ide)(643) and Pchlide(655) as well as an emission band at 688 nm corresponding to a chlorophyll form. The Pchlide(655) was fully photoactive in these species. The outermost leaf primordia of these four species and the innermost leaf primordia of 28 other species contained all of the above described Pchl(ide) forms in various ratios but in small amounts. In addition, Chl forms were present and the main bands in the fluorescence emission spectra were at 690 or 740 nm, or both. The results indicate that Pchl(ide) accumulation occurs in leaf primordia in near darkness inside the tightly closed buds, where the bud scales and the external leaf primordia function as optical filters.     

三种松树叶绿素荧光特征比较研究

以赤松、油松、白皮松3种松树为材料,利用便携式调制荧光仪MINI-PAM测定自然条件下其各叶绿素荧光参数Ft、Fm、Yield、ETR、qP、qN或NPQ和PAR指标的日变化。结果表明:3种松树各个时段荧光参数值在总体上差异不大,叶绿素荧光参数的变化依赖于PAR的变化。Ft、rETR、qN和NPQ的变化与PAR的变化趋势一致,Fm、Yield和qP的变化与PAR的变化相反。其中,白皮松的量子产量Yield、相对光合电子传递速率ETR、光化学淬灭系数qP、非光化学淬灭系数qN和NPQ均高于赤松和油松,说明其能通过增加热耗散来尽量降低光抑制程度,对当地环境较为适应。本研究为3种松树抗性研究、苗木管理、科学种植等提供了生理指标和依据。     

Frost resistance and ice nucleation in leaves of five woody timberline species measured in situ during shoot expansion

Frost resistance and ice nucleation temperatures of leaves, from bud swelling until after full expansion, were measured in situ for five major woody timberline species with recently developed field freezing equipment. Frost resistance determined in situ on leaves of attached twigs was significantly higher than values determined on detached leaves in laboratory tests (e.g., the temperature at which incipient frost damage was observed (LTi) was 1.2 degrees C higher for detached leaves than for attached leaves of Picea abies (L.) Karst.). Frost resistance of leaves of all species changed significantly during shoot expansion (e.g., changes of 7.2 and 11 degrees C for Rhododendron ferrugineum L. and Larix decidua Mill., respectively). Expanding leaves (between 0 and 60% of full expansion) were the most sensitive to frost, with LTi values ranging from -3.4 degrees C in R. ferrugineum to -6.3 degrees C in L. decidua. Among the studied species, P. abies and R. ferrugineum were the most frost sensitive throughout the shoot elongation period. In situ freezing patterns of leaves of attached twigs also differed from those of leaves of excised twigs. During leaf expansion, two distinct freezing exotherms were always registered in situ. The first freezing event (E1, high-temperature exotherm) was recorded at -1.5 +/- 0.2 degrees C and reflected extracellular ice formation. Exposure of leaves to temperatures at which E1 occurred was, in all cases, noninjurious. The low-temperature exotherm (E2) mostly coincided with frost damage, except for some stages of leaf expansion in R. ferrugineum and P. abies, indicating that in situ freezing exotherms were not accurate estimators of frost damage in these species.     

Masting in Fagus crenata and its influence on the nitrogen content and dry mass of winter buds

In Fagus, full-mast seeding years are invariably followed by at least one non-mast year. Both flower and leaf primordia develop during the summer within the same winter buds. Flower bud initiation occurs when the N content of developing seeds is increasing rapidly. We hypothesized that competition for nitrogen (N) between developing seeds and buds limits flower primordium formation in mast years and, hence, limits seed production in years following mast years. We tested this hypothesis in three Fagus crenata Blume forests at elevations of 550, 900 and 1500 m. Bud N concentration (N con), amount of N per bud (N bud) and dry mass per bud (DM) were compared between a mast year (2005) and the following non-mast year (2006), and between winter buds containing both leaf and flower primoridia (BF), which were formed during the non-mast year, and winter buds containing leaf primordia only (BL), which were formed in both mast and non-mast years. In addition, leaf numbers per shoot corresponding to the analyzed buds were counted, and the effect of masting on litter production was analyzed by quantifying the amounts of litter that fell in the years 2004 to 2007. The dry mass and N content of BF formed in 2006 by trees at both 550 and 1500 m were 2.1-3.4-fold higher than the corresponding amounts in BL, although the numbers of leaves per current-year shoot in 2007 that developed from the two bud types in the same individuals did not differ significantly. These results indicate that more N and carbohydrate are expended in producing BF than in producing BL. The amount of litter from reproductive organs produced in the mast year was similar to the amount of leaf litter at 900 and 1500 m, but three times as much at 550 m. Leaf numbers per shoot were significantly lower at all elevations in the mast year than in the non-mast years (and the amount of leaf litter at 550 and 1500 m tended to be lower in the mast year than in the non-mast years. In conclusion, preferential allocation of resources to seeds in the mast year reduced the availability of resources for flower primordium formation, and this may have accounted for the poor seed production in the following non-mast year.     

Transient etiolation: protochlorophyll(ide) and chlorophyll forms in differentiating plastids of closed and breaking leaf buds of horse chestnut (Aesculus hippocastanum)

An accompanying paper reports the accumulation of photoactive protochlorophyllide (Pchlide) in the innermost leaf primordia of buds of many tree species. In this paper, we describe plastid differentiation, changes in pigment concentrations and spectral properties of bud scales and leaf primordia of horse chestnut (Aesculus hippocastanum L.) from January until the end of bud break in April. The bud scales contained plastids with grana, stroma thylakoids characteristic of chloroplasts and large dense bodies within the stroma. In January, proplastids and young chloroplasts were present in the leaf primordia, and the fluorescence spectra of the primordia were similar to those of green leaves except for a minor band at 630 nm, indicative of a protochlorophyll(ide). During bud break, the pigment concentrations of the green bud scales and the outermost leaf primordia increased, and Pchlide forms with emission maxima at 633, 644 and 655 nm accumulated in the middle and innermost leaf primordia. Depending on the position of the leaf primordia within the bud, their plastids and their pigment concentrations varied. Etio-chloroplasts with prolamellar bodies (PLBs) and prothylakoids with developing grana were observed in the innermost leaves. Besides the above-mentioned Pchlide forms, the middle and innnermost leaf primordia contained only a Chl band with an emission maximum at 686 nm. The outermost leaf primordia contained etio-chloroplasts with well-developed grana and small, narrow-type PLBs. These outermost leaves contained only chlorophyll forms like the mature green leaves. No Pchlide accumulation was observed after bud break, indicating that etiolation of the innermost and middle leaves is transient. The Pchlide forms and the plastid types of the primordia in buds grown in nature were similar to those of leaves of dark-germinated seedlings and to those of the leaf primordia of dark-forced buds. We conclude that transient etiolation occurs under natural conditions. The formation of PLBs and etio-chloroplasts and the accumulation of the light-dependent NADPH:protochlorophyllide oxidoreductase are involved in the natural greening process and ontogenesis of young leaf primordia of horse chestnut buds.     

Hydraulic architecture correlates with bud organogenesis and primary shoot growth in beech (Fagus sylvatica)

In beech (Fagus sylvatica L.), the number of leaf primordia preformed in the buds determines the length and the type (long versus short) of annual growth units, and thus, branch growth and architecture. We analyzed the correlation between the number of leaf primordia and the hydraulic conductance of the vascular system connected to the buds. Terminal buds of short growth units and axillary buds of long growth units on lower branches of mature trees were examined. Buds with less than four and more than five leaf primordia formed short and long growth units, respectively. Irrespective of the type of growth unit the bud was formed on, the occurrence of a large number of leaf primordia was associated with high xylem hydraulic conductance. Xylem conductance was correlated to the area of the outermost annual ring. These results suggest that organogenesis and primary growth in buds correlates with secondary growth of the growth units and thus with their hydraulic architecture. Possible causal relationships between the variables are discussed.     

暖温带石灰岩山地森林种群格局的分形特征研究

从空间占据的角度入手,计算了研究区域内4种主要林分共6块样地的计盒维数。计算结果表明6块样地均存在贯穿于研究尺度的自相似规律,其中赤松林样地的计盒维数最大,而刺槐林样地的计盒维数差异较大。计盒维数的大小和不同植被的生态学特性和立地条件有关。最后从空间占据的角度对区域生态安全格局的维持和生态恢复提出了建议。     

Micropropagation of Pinus densiflora and the evaluation of nematode resistance of regenerated microshoots in vitro

To accelerate the breeding and selection of Pinus densiflora Siebold and Zucc. resistance to pine wilt disease, a micropropagation system was established and nematode resistance evaluated in vitro. Cotyledon-hypocotyl explants from 28-day-old seedlings were first cultured on Gresshoff and Doy medium supplemented with 4.0 mg L^-1 6-benzyladenine and 0.2 mg L^-1 a-naphthaleneacetic acid(NAA) to stimulate the formation of buds. Induced buds were subsequently subcultured on Gupta and Durzan medium supplemented with 0.1%(w/v)activated charcoal for elongation. Stem sections derived from shoots were used as explants for the further multiplication. Roots were formed from shoots transferred to woody plant medium containing 0.2 mg L^-1 NAA for4 weeks. The nematode resistance test showed that symptoms in micropropagated shoots after infection with pine wood nematode(PWN) were similar to those in plants infected in the field. The wilting rate varied from 20 to100% among different clones 18 days after inoculation.The most susceptible clone was Clone 6-4 with a 100%wilting rate, while Clone 8-4 showed a relatively high resistance with a 20% wilting rate. The number of nematodes recovered from Clone 8-4 shoots was significantly lower(P = 0.05) than from Clones 5-10 and 16-4. This work contributes to the breeding of PWN resistance in P.densiflora.     

Effect of GA(4 + 7) on the initiation and development of the inflorescence bud of evergreen azalea

Apical buds of evergreen azalea (Rhododendron sp.) were treated with GA(4 + 7) at different stages of development. Treatment of vegetative buds stimulated shoot growth, slightly delayed both flower initiation and development, but increased the number of flower primordia. Treatment at the time of floral transition induced bud abortion at an early stage of the reproductive development. Treatment of inflorescence buds which contained at least one complete flower substituted for chilling in overcoming dormancy and prevented inflorescence bud abortion.     

Hydraulic conductivity, photosynthesis and leaf water balance in six evergreen woody species from fall to winter

To confirm that freeze-thaw embolism is a primary stress for evergreen woody species in winter, hydraulic conductivity, photosynthesis and leaf water potential were measured during fall and winter in trees growing in a cool temperate zone (Nikko) and in a warm temperate zone (Tokyo). We examined two evergreen conifers that naturally occur in the cool temperate zone (Abies firma Siebold & Zucc. and Abies homolepis Siebold & Zucc.), and four evergreen broad-leaved woody species that are restricted to the warm temperate zone (Camellia japonica L., Cinnamomum camphora (L.) J. Presl, Ilex crenata Thunb. and Quercus myrsinaefolia Blume). In Tokyo, where no freeze-thaw cycles of xylem sap occurred, hydraulic conductivity, photosynthesis and water balance remained constant during the experimental period. In Nikko, where there were 38 daily freeze-thaw cycles by February, neither of the tracheid-bearing evergreen conifers showed xylem embolism or leaf water deficits. Similarly, the broad-leaved evergreen trees with small-diameter vessels did not exhibit severe embolism or water deficits and maintained CO(2) assimilation even in January. In contrast, the two broad-leaved evergreen trees with large-diameter vessels showed significantly reduced hydraulic conductivity and shoot die-back in winter. We conclude that freeze-thaw embolism restricts evergreen woody species with large-diameter vessels to the warm temperate zone, whereas other stresses limit the distribution of broad-leaved trees, that have small-diameter vessels, but which are restricted to the warm temperate zone.     

Spatial and seasonal variability of temperature responses of biochemical photosynthesis parameters and leaf nitrogen content within a Pinus densiflora crown

We measured seasonal variation in area-based nitrogen concentration (N), maximum rate of carboxylation (Vcmax) and maximum rate of electron transport (Jmax) in 1-year-old needles along four first-order branches within a Pinus densiflora Sieb. & Zucc. crown, and analyzed their relationships to growth irradiance and temperature. Each leaf light environment was expressed as a ratio of the monthly mean of daily integrated photosynthetically active irradiance (Iint) for the particular needle to Iint above the canopy (Irel). Needle N decreased in the upper crown during the development of new needles, whereas it remained fairly constant in the lower crown, reflecting differences between upper and lower crown needles in their contribution to the nitrogen of new needles. Gradients of N within the crown were correlated with Irel in all seasons (r2 = 0.40-0.78). Seasonal variation in N was weakly correlated with mean daily air minimum temperatures. Both Vcmax and Jmax showed seasonal variation in all first-order branches, and decreased to their lowest values in winter. The gradients of Vcmax and Jmax within the crown were not correlated with Irel in some seasons, but were correlated with changes in N in most months (r2 = 0.33-0.75), except in the winter. Furthermore, the regression slope of the relationship between N and Vcmax and the temperature response of Vcmax and Jmax exhibited seasonal variation.     

Horizontal and vertical variations in photosynthetic capacity in a Pinus densiflora crown in relation to leaf nitrogen allocation and acclimation to irradiance

We measured horizontal and vertical gradients of light (rPPFD) along four first-order branches of a Pinus densiflora Sieb. & Zucc. crown, and compared variations in specific leaf area (SLA), needle nitrogen concentration (N), chlorophyll concentration (Chl) and photosynthetic capacity (i.e., maximum rate of carboxylation (V(cmax))) along the two axes. The horizontal gradient of rPPFD along first-order branches was similar in magnitude to the vertical gradient of rPPFD from the upper to the lower crown. None of the measured parameters (i.e., SLA, N, Chl and Vcmax) were strictly proportional to rPPFD, although they were more or less correlated with light when data obtained for all of the crown were pooled (r(2) = 0.31-0.80). The slope of rPPFD against N on an area basis (Narea) for a branch in the middle of the crown orientated northward was significantly greater than the slope for a similar branch orientated southward. Horizontal variations were unrelated to age effects because measurements were all on 1-year-old needles. We conclude that factors other than light (i.e., orientation) may influence N allocation within branches. There was considerably less variation in the relationship of Vcmax to Narea (r2 = 0.58) than in the relationship of Vcmax to rPPFD (r2 = 0.41). Fractional N distribution among components of the photosynthetic machinery was constant within the crown. Together with the relationships between rPPFD and N on a mass basis (r2 = 0.80) and SLA and Vcmax (r2 = 0.60), these findings suggest that most light acclimation in P. densiflora occurs through changes in needle morphology (e.g., SLA) during development.     

Physiological, environmental and genetic variation in apical dominance as determined by decapitation in Triplochiton scleroxylon

Patterns of lateral shoot growth following decapitation in 1-meter tall, rooted Triplochiton scleroxylon K. Schum. cuttings varied with clone and in response to a range of environmental conditions and growth regulator treatments. Two phases of bud activity were identified, the Sprouting Phase, in which many buds were released from correlative inhibition, and the Dominance Phase (starting 3-4 weeks after decapitation) in which uppermost laterals began to dominate and suppress growth, and sometimes cause apical abscission of lower lateral shoots. Except in non-erect plants, the most distal lateral to elongate became the new leading shoot. During the Sprouting Phase, the proportion of active buds was increased by removing leaves from the upper part of the stem, and by reducing the photoperiod from 13-15 h to 11 h, particularly at 20 degrees C rather than 25 degrees C. Conversely, the proportion of sprouting buds was decreased by injecting plant stems with NAA (250 microg/plant). During the Dominance Phase, suppression of lateral shoot growth was hastened by stem injection with GA(3) (200 microg/plant), especially when applied to the uppermost shoot at the end of the Sprouting Phase. Reimposition of dominance was delayed, however, by (1) high rates of N:P:K fertilization, (2) low temperature (20 versus 25 degrees C) under relatively long days (13 and 15 h), (3) low photon flux density (160 micromol m(-2) s(-1)) and (4) severe defoliation. Plant orientation had no effect on bud activity of decapitated plants, but affected the relative vigor and orientation of new lateral shoots. High temperature (25 versus 20 degrees C) and injection with GA(3) increased the erectness of newly developing lateral shoots.     

红松山地育苗和平地育苗效果的研究

通过几年山地和平地红松育苗试验,概括地总结了不同育苗地点、不同播种量、不同肥料种类对红松苗木的生长量及产量的影响,从试验结果看出,山地繁育红松苗比平地在生长量和苗木产量上都有较明显的提高,不同的肥料种类对红松苗的生长量有较大的差异,三元复合肥和腐熟鸡粪较单一化肥和不施肥的试验对照育苗效果较好。     

红松引种中间试验研究

在呼伦贝尔市引种红松，其地理位置及诸多气象因子等与原产地有很大差异，因此将小兴安岭温带湿润地区的树种引种到寒带或温带半湿润的大兴安岭地区进行栽培，要经过科学的试验和采取相应的驯化措施，才能达到引种目的。本文通过在大兴安岭地区引种红松的中间试验，找到了一种适合当地栽植的方法，为今后寒带或温带半湿润地区的红松栽植提供了一条有效的途径。