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1.
本试验旨在研究用于肉鸡小麦-豆粕型饲粮的木聚糖酶、β-葡聚糖酶、纤维素酶、植酸酶复配的最佳酶谱组合。试验首先采用单因素完全随机试验设计,将木聚糖酶、β-葡聚糖酶、纤维素酶、植酸酶分别设定6个水平添加到肉鸡小麦-豆粕型饲粮中,采用模拟胃肠液体外消化试验,研究单酶的最佳剂量;然后根据各单酶最佳添加量,将4种单酶分别设定3个水平:木聚糖酶(750、800、850 U/g),β-葡聚糖酶(50、100、150 U/g),纤维素酶(700、750、800 U/g),植酸酶(1 000、1 500、2 000 U/kg),采用4因子3水平L9(34)正交设计,设置9种组合方式,以净还原糖生成量、植酸磷降解率、饲粮残渣总能为检测指标,确定4种单酶复配的最佳酶谱组合。结果表明:肉鸡小麦-豆粕型饲粮中木聚糖酶、β-葡聚糖酶、纤维素酶和植酸酶的最佳添加量,0~3周龄分别为800、130、770、1 360 U/kg;4~6周龄分别为800、130、790、1 470 U/kg;4种单酶组合的最佳酶谱均为木聚糖酶850 U/g、纤维素酶750 U/g、β-葡聚糖酶50 U/g、植酸酶2 000 U/kg。  相似文献   

2.
本试验旨在对比研究酸性和中性木聚糖酶的酶学性质及其在小麦型饲粮中的降黏效果。饲养试验检测2种木聚糖酶(酸性和中性木聚糖酶)对胃肠液的耐受性;pH 3.5条件下测试2种木聚糖酶的相对活性;选用木聚糖纯品和谷物检测2种木聚糖酶的降黏效果;选取512只1日龄的817白羽肉公鸡,随机分为4组(每组8个重复,每个重复16只),对照组饲喂小麦型基础饲粮,试验组在小麦型基础饲粮中分别添加4 U/g中性木聚糖酶、2和4 U/g酸性木聚糖酶,试验期42 d。结果显示:1)相比中性木聚糖酶,酸性木聚糖酶对肉鸡胃肠液有更好的耐受性。2)在pH 3.5条件下,酸性木聚糖酶的相对活性比中性木聚糖酶高64.1%。3)对于高黏(黑麦)阿拉伯木聚糖,相比中性木聚糖酶,酸性木聚糖酶的降黏效果提升了42.9%。4)在小麦型饲粮中添加木聚糖酶提高了肉鸡的生长性能,其中4 U/g酸性木聚糖酶对生长性能的提高效果优于4 U/g中性木聚糖酶。5)对于饲喂小麦型饲粮的肉鸡,4 U/g酸性木聚糖酶组粗蛋白质表观消化率分别比2 U/g酸性木聚糖酶组、4 U/g中性木聚糖酶组和对照组提高了3.5%(P<0.05)、7.4%(P&l...  相似文献   

3.
家禽小麦型日粮添加木聚糖酶体外消化的效果研究   总被引:1,自引:0,他引:1  
运用体外模拟家禽消化装置,通过测定5个木聚糖酶水平下3种比例的小麦型日粮体外消化产生还原糖的量,研究不同水平木聚糖酶在家禽小麦型日粮中的酶解效果。试验表明:小麦水平B组日粮添加木聚糖酶的最佳水平为0.135%,小麦水平C组日粮添加木聚糖酶的最佳水平为0.204%;小麦水平D组日粮添加木聚糖酶的最佳水平为0.243%。  相似文献   

4.
不同非淀粉多糖复合酶在肉鸡小麦型饲粮中的应用效果   总被引:1,自引:0,他引:1  
本试验旨在研究低能量水平的小麦型饲粮中添加不同非淀粉多糖(NSP)复合酶对肉鸡生长性能、消化道生理、屠宰性能、肠道微生物数量及经济效益的影响,以确定适宜的NSP复合酶。试验采用单因子完全随机设计,选取900只1日龄的科宝肉公鸡,按体重无差异原则随机分为5个组,正对照组(PC组)饲喂正常能量水平的基础饲粮;负对照组(NC组)饲喂在PC组饲粮的基础上降低350 kJ/kg能量水平的饲粮;NSP复合酶组饲喂在NC组饲粮中分别添加NSP复合酶1(300 g/t)、NSP复合酶2(300 g/t)、NSP复合酶3(500 g/t)的饲粮,每个组10个重复,每个重复18只鸡。试验分为前期(1~21 d)、后期(22~42 d)2阶段进行。结果表明:1)各组在试验各阶段的平均体重、平均体增重、平均采食量、料重比差异均不显著(P>0.05);与PC组相比,NC组平均体重、平均体增重略低(P>0.05),料重比均增加(P>0.05),而添加NSP复合酶后有所改善。2)各组第21和42天的肌胃、腺胃、十二指肠、空肠、回肠、盲肠相对重量差异均不显著(P>0.05);与NC组相比,NSP复合酶1组、NSP复合酶2组第21和42天的空肠、回肠相对重量降低(P>0.05),盲肠相对重量提高(P>0.05)。各组第21和42天的十二指肠、空肠、回肠、盲肠相对长度差异均不显著(P>0.05);与PC组相比,NC组各肠段相对长度(第21天十二指肠相对长度除外)均提高(P>0.05),与NC组相比,NSP复合酶1组、NSP复合酶2组第21和42天的空肠、回肠相对长度均降低(P>0.05)。3)试验第21天,与PC组相比,NSP复合酶组十二指肠隐窝深度显著提高(P<0.05),NC组空肠隐窝深度显著提高(P<0.05);各组间各肠段肌层厚度差异均不显著(P>0.05)。试验第42天,与PC组相比,其余组十二指肠绒毛高度均降低(P>0.05);与NC组相比,添加NSP复合酶后空肠绒毛高度显著降低(P<0.05)。4)各组屠宰性能差异均不显著(P>0.05);与PC组相比,NC组的腹脂率有所下降(P>0.05),与NC组相比,NSP复合酶3组屠宰率、半净膛率、全净膛率、胸肌率均有所提高(P>0.05)。5)各组第7、21、42天的大肠杆菌、乳酸杆菌、双歧杆菌数量差异均不显著(P>0.05)。与PC组相比,NC组第7、42天大肠杆菌、乳酸杆菌数量和第7、21、42天双歧杆菌数量有所降低(P>0.05)。与NC组相比,NSP复合酶1组、NSP复合酶2组第42天乳酸杆菌、双歧杆菌数量有所提高(P>0.05)。6)以PC组利润为100%,与之相比,NSP复合酶1组提高的利润幅度最大。从结果中可以看出,在小麦型饲粮中降低一定能量水平后添加NSP复合酶可在一定程度上改善肉鸡生长性能、肠道形态和肠道微生态环境,并可不同程度地提高养殖经济效益。综合考虑,本试验中NSP复合酶1的应用效果最优。  相似文献   

5.
本试验研究了小麦型日粮中添加不同酶制剂对黄羽肉鸡生长性能的影响。试验选取3600只快大型1日龄黄羽肉鸡,随机分为6个组,每组6个重复,每个重复100只鸡。试验分为6个组:A组为玉米-豆粕型日粮组(作为正对照组);B组为玉米-小麦-豆粕型日粮组(作为负对照组);C、D、E、F组为在负对照组基础上分别添加250g/t酶制剂产品Ⅰ、Ⅱ、Ⅲ、Ⅳ,试验为期51天,分1-21日龄与22-51日龄两个阶段。试验结果表明:21日龄日增重最小的是负对照组,分别比正对照组、试验C、D、E低4.32%、6.50%、4.62%、2.49%,均差异显著(P0.05),增重最大的为C组。从整个生长期来看,日增重最低的是负对照组,比试验C组低3.05%,差异显著(P0.05)。日增重最高的是C组,但与其他酶制剂添加组的D、E、F组之间无显著性差异(P0.05)。  相似文献   

6.
<正>研究表明,家禽日粮中添加木聚糖酶可提高日增重与饲料转化效率[1-3]。木聚糖酶的作用效果受很多因素的影响,导致其应用中的差异很大。其中能量水平是影响木聚糖酶作用效果的重要因素之一。日粮营养  相似文献   

7.
为研究小麦替代玉米作为能量饲料对母猪一个繁殖周期生产性能的影响,选取遗传背景相同、胎次及妊娠期一致、体况良好、生产性能相近的经产大长母猪60头,随机分为5个处理,即对照组、无酶试1组、低酶试2组、中酶试3组、高酶试4组,每个处理3个重复,每个重复4头母猪。试验结果表明,无酶试1组产活仔数显著提高(P〈0.05);初生重、初生窝重、健仔率与对照组相比差异不显著(P〉0.05),母猪断奶1周内发情配种率比对照组提高8.34个百分点,差异显著(P〈0.05);仔猪断奶成活率显著提高(P〈0.05);断奶重、断奶窝重、日增重与对照组相比均差异不显著(P〉0.05)。3个加酶组母猪产活仔数、初生重、初生窝重、健仔率、母猪断奶1周内发情配种率显著或极显著高于对照组;仔猪日增重、断奶窝重、断奶成活率均明显提高(P〈0.05),其中中酶试3组、高酶试4组仔猪断奶成活率极显著高于对照组(P〈0.01)。说明用添加复合酶的小麦豆粕型饲粮饲养母猪是可行的,不仅降低了饲养成本,还提高了生产效益,母猪在一个繁殖周期添加中等水平复合酶经济效益提高620.10元/头,增幅达86.9%(P〈0.01)。  相似文献   

8.
本试验旨在探讨不同粗饲料间的组合效应以及筛选出的最佳组合与精料固定比例组合对体外发酵参数的影响。利用体外产气法,将酱香型发酵酒糟、浓香型干酒糟、浓香型湿酒糟、酱香型湿酒糟分别与稻草、燕麦干草以0∶100、25∶75、50∶50、75∶25、100∶0的比例组合,分析体外发酵主要指标:产气量(GP)、pH、微生物蛋白(MCP)含量以及48 h产气量的单项组合效应指数(SFAEI);根据SFAEI,再将4种白酒糟与稻草、燕麦干草的最佳组合与相同精料混合(精粗比6∶4),分析体外发酵效果。结果表明:1)4种白酒糟与2种干草组合均能产生正组合效应,且与燕麦干草的组合效应优于与稻草的组合。2)最佳组合比例及SFAEI为:酱香型发酵酒糟∶燕麦干草=25∶75、SFAEI=11.48,浓香型干酒糟∶燕麦干草=25∶75、SFAEI=3.95,浓香型湿酒糟∶燕麦干草=50∶50、SFAEI=6.70,酱香型湿酒糟∶燕麦干草=25∶75、SFAEI=6.47。3)相同比例饲粮条件下,酱香型发酵酒糟组(60%精料+10%酱香型发酵酒糟+30%燕麦干草)甲烷产量显著低于浓香型湿酒糟组(60%精料+20%浓香型湿酒糟+20%燕麦干草)和酱香型湿酒糟组(60%精料+10%酱香型湿酒糟+30%燕麦干草)(P<0.05),酱香型发酵酒糟组和浓香型干酒糟组(60%精料+10%浓香型干酒糟+30%燕麦干草)的二氧化碳产量也显著低于其余2个湿酒糟组(P<0.05),而挥发性脂肪酸(VFA)含量则显著高于2个湿酒糟组(P<0.05)。综上所述,根据SFAEI,酱香型发酵酒糟、浓香型干酒糟、酱香型湿酒糟与燕麦干草组合的最佳比例是25∶75,浓香型湿酒糟与燕麦干草组合的最佳比例是50∶50;根据气体成分及总VFA含量,酱香型发酵酒糟组和浓香型干酒糟组体外发酵效果更优。  相似文献   

9.
<正>动物的生长受神经内分泌系统的调节,其中最重要的是由下丘脑-垂体-靶腺构成的神经内分泌生长轴。而生长激素是该调控的核心,它能促进蛋白质的合成,抑制脂肪组织对葡萄糖的摄取和脂肪的合成[1]。生长抑素是神经系统和肠道产生的,  相似文献   

10.
戊聚糖酶在肉鸡小麦-豆粕型日粮中的应用   总被引:2,自引:0,他引:2  
1 材料与方法1.1 试验用酶制剂 试验所用的商品酶制剂为“特威宝PT酶制剂”(Allzyme PT),其主要成分为戊聚糖酶(阿拉伯木聚糖酶),活性为2000 XU/g。1.2 试验动物与分组 挑选体重、大小基本一致的1日龄艾维茵商品代鸡苗600只,随机分为4个处理组。A1组:河套  相似文献   

11.
对肉仔鸡体外消化模拟技术评定不同剂量植酸酶在玉米-豆粕日粮中作用效果的可行性进行了检验.在体外条件下,分别测定了添加与不添加植酸酶的6种玉米-豆粕日粮磷体外消化率和无机磷释放量.结果表明:磷体外消化率和无机磷释放量随植酸酶添加水平的增加而增加(P<0.01),添加不同剂量的植酸酶日粮,磷体外消化率和无机磷释放量与肉仔鸡日增重、采食量、回肠磷消化率、胫骨灰分和磷的沉积以及胫骨灰分中磷的含量等指标均存在强相关(R^2=0.971,0.990,0.962,0.903,0.866,0.986和R^2=0.970,0.987,0.976,0.898,0.859,0.984).利用肉仔鸡体外消化模拟技术,可以评定和预测玉米-豆粕日粮中添加不同剂量植酸酶对肉仔鸡生产性能和磷利用的影响.  相似文献   

12.
Two experiments were conducted with broiler chicks to determine the nutritive value of high-oil sunflower meal (HO-SFM), a sunflower oil extraction by-product obtained through screw-press extraction and expanding processes with a proximate composition of 32% CP, 12% crude fiber, and 19% ether extract. In Experiment 1, the effects of a high level (46.4%) of HO-SFM on chick performance and gastrointestinal organs were tested. The objective of Experiment 2 was to determine if pelleting the feed could overcome the bulkiness resulting from inclusion of HO-SFM at a high level. The results of Experiment 1 indicated that addition of 46.4% HO-SFM to broiler starter diets significantly (P < 0.05) depressed body weight, feed intake, and gain but not feed conversion. Fat pad and liver lipid were again significantly (P < 0.05) decreased in the HO-SFM treatment. Impaired performance might have been due to the difference of the density of HO-SFM diet (608 g/L) compared with the soybean meal control (723 g/L). When bulkiness was overcome by pelleting in Experiment 2, it was found that pelleting the feed significantly enhanced growth of broiler chicks compared with SFM or soybean meal mash diets. Liver weights and lipid content were again decreased in HO-SFM diets. The results of this study suggest that HO-SFM can be used up to 28% without adverse effects on broiler chicks. Further improvement was observed with pelleting. Liver weight and lipid content were consistently reduced by feeding HO-SFM.  相似文献   

13.
Two experiments were conducted under hot and cool seasons to investigate the effects of multiple-enzyme mixtures on the growth performance of broilers fed reduced energy corn-soybean meal diets. Broilers responded to enzyme supplementation with greater magnitude in the hot season (experiment 1) than in the cool season (experiment 2). Adding adequate activity levels of α-amylase, β-glucanase, and xylanase to broiler starter and grower corn-soybean diets with a 3% reduction in dietary ME allowed full restoration of growth performance of broilers comparable to those fed the adequate energy positive control diet regardless of season.  相似文献   

14.
Due to its low nutrient density, cottonseed meal (CSM) may be a potential alternative feed ingredient for soybean meal (SBM) in broiler breeder pullet diets to improve flock BW uniformity. A major concern when utilizing CSM in poultry diets, however, is the presence of gossypol. It was determined in an initial experiment that when broiler breeder hens are fed a diet containing CSM, gossypol accumulates in the liver. When CSM is removed from the diet, however, hepatic gossypol levels dissipate within a few weeks. In a subsequent study, in order to achieve the breeder guideline ideal weight, broiler breeder pullets reared with a diet containing CSM from 2 to 18 wk of age had to be fed a larger amount than birds consuming a standard SBM diet. The CV of bird weight was significantly lower during the rearing period for birds reared with the corn and CSM diet. This difference in uniformity was maintained as the hens entered the breeding period. Egg weight, hatchability, and fertility were equivalent for hens reared with a diet containing CSM and those reared with a standard diet throughout the growing period. The results suggest that broiler breeder pullets reared on a diet containing CSM as the major protein source have improved flock BW uniformity and normal subsequent reproductive performance.  相似文献   

15.
针对饲粮非淀粉多糖(NSP)酶的海量筛选工作和动物试验间的可比性差等问题,本研究探讨使用体外模拟法优化生长猪玉米-豆粕型饲粮和玉米-杂粕型饲粮的NSP酶谱。首先采用单因素随机试验设计,研究NSP酶的添加水平与饲粮体外干物质消化率(IVDMD)的关系。在玉米-豆粕型饲粮和玉米-杂粕型饲粮中分别添加不同水平的纤维素酶、木聚糖酶、β-葡聚糖酶、β-甘露聚糖酶、α-半乳糖苷酶和果胶酶6种NSP酶,分析各NSP酶对饲粮IVDMD的作用效果。然后采用二次回归旋转正交组合试验设计,筛选2种饲粮中6种NSP酶的最佳酶谱。结果表明:1)6种NSP酶的添加水平与2种类型猪饲粮IVDMD之间存在二次曲线关系。2)α-半乳糖苷酶对玉米-豆粕型饲粮的IVDMD提升最高,达到了1.28%,木聚糖酶对玉米-杂粕型饲粮的IVDMD提升最高,达到了1.95%。3)玉米-豆粕型饲粮的最佳酶谱为:纤维素酶533.6 U/kg、木聚糖酶9 983.7 U/kg、β-葡聚糖酶1 014.4 U/kg、β-甘露聚糖酶4 080.6 U/kg、α-半乳糖苷酶251.6 U/kg和果胶酶107.3 U/kg。玉米-杂粕型饲粮的最佳酶谱为:纤维素酶960.0 U/kg、木聚糖酶17 177.6 U/kg、β-葡聚糖酶405.8 U/kg、β-甘露聚糖酶19 023.2U/kg、α-半乳糖苷酶307.2 U/kg和果胶酶96.9 U/kg。4)优化后的酶谱使玉米-豆粕型饲粮的IVDMD提升了3.26%,使玉米-杂粕型饲粮的IVDMD提升了3.75%。由此可见,6种NSP酶联合使用能够更大程度地提高生长猪玉米-豆粕型饲粮和玉米-杂粕型饲粮的IVDMD。  相似文献   

16.
将过3.0 mm、2.0 mm、1.2 mm和0.8 mm孔径筛片的蒸炒豆粕(测定的几何平 均粒度分别为2 565μm、529μm、449μm、334μm和210μm)进行体外消化,研究豆粕粒度 与蛋白质消化率的关系,结果表明:粒度为449μm组的粗蛋白消化率最高,比2 565μm、529 μm和334μm组分别提高了10.75%、4.43%和1.37%(P<0.01);比210μm组提高了 0.17%(P<0.05);粒度为449μm组和334μm组的干物质消化率(分别为68.95%, 68.89%)较高,分别比2 565μm和529μm组提高了(3.52%,3.44%)和(8.14%,8.06%) (P<0.01);粒度为449μm的豆粕的赖氨酸、精氨酸、胱氨酸、甘氨酸、谷氨酸、天门冬氨酸、丙 氨酸、酪氨酸和脯氨酸的消化率均高于其他粒度组。  相似文献   

17.
Diets containing low-quality durum wheat (716 g/kg) for production of pasta were used to feed male broiler chicks. The efficacy of xylanase supplementation and the impact of xylanase inhibitors on losses in exogenous enzyme activity were analyzed. Birds fed on the basal diet, not supplemented with recombinant xylanases or Roxazyme G, reached a BW of 1,509 g with a feed conversion ratio of 1.77 at d 28. Growing performance was above that expected for the breed used, whereas feed conversion ratios were relatively higher. None of the 3 xylanase preparations under analysis affected growing performances and feed efficiency of broiler chicks. The activity of feed xylanases was considerably reduced in the presence of durum wheat extracts. The results suggest that reduction of exogenous enzyme activity was due to the action of durum wheat xylanase inhibitors and not to proteolysis.  相似文献   

18.
Soybean meal contains approximately 0.62% total P of which 0.4% can be phytate P, which is considered less biologically available for poultry than other forms of P. Soybean meal is a key ingredient in poultry feeds and information is needed about the range of phytate P and nonphytate P in different soybean meals. The phytate P content of soybeans may vary due to climatic conditions, soil type and soybean variety. Previous research has shown that phytate P can be hydrolyzed in the gastrointestinal tract providing available P by adding a commercial phytase enzyme to poultry feed. The extent of phytate hydrolysis by dietary supplementation of phytase has been shown to vary depending on the type of dietary ingredients such as corn, soybean meal, canola meal, and wheat. Research is needed to determine if different commercially available soybean meals respond in a similar manner to a feed added phytase. Twenty-five soybean meal samples were collected from active soybean crushing plants in the United States and 18 of the samples were selected to evaluate the effect of a microbial phytase on phytate P disappearance using 5-d bioassays. The range of analyzed values in soybean meal samples for total P, phytate P, Ca, protein, and neutral detergent fiber (NDF) were 0.59 to 0.87, 0.32 to 0.42, 0.28 to 0.54, 40.44 to 51.69, and 7.78 to 16.09%, respectively. Bioassay results indicate that body weight, feed consumption, and feed conversion ratio improved significantly (P < 0.05) in some of the groups fed diets with enzyme compared with groups fed the same diet with no added enzyme. The range of total P retention and phytate P disappearance for groups fed diets with no enzyme were 21.35 to 48.41 and 13.64 to 37.13%, respectively. The addition of phytase increased total P retention and phytate P disappearance from 56.81 to 68.62 and 76.18 to 94.08%, respectively. The results indicate no correlation among components (total P, phytate P, Ca, protein, and NDF) of soybean meal samples, percentage of phytate P disappearance, and percentage of total P retention for groups fed diets with and without added phytase.  相似文献   

19.
Nutritional and management interventions are needed to reduce the incidence of pododermatitis in poultry. In this study, enzyme (Allzyme Vegpro) supplementation of corn-soybean meal-based broiler diets was evaluated in an effort to reduce total N and NH3 production and its effect on pododermatitis in broiler chickens. A total of 1,600 mixed-sex chicks were raised on floor pens in a design involving 2 × 2 × 2 arrangement of protein level [high or low], protein source [all vegetable (Veg) or vegetable plus animal (Veg + Ani)], and enzyme [with or without enzyme supplementation (0.06%)], on a 4-stage feeding program (4 replicate pens/treatment; 50 birds/pen). In addition to live performance, the feet were scored for incidence of lesions on all birds on 28, 42, and 57 d of age, and the severity was recorded as none, mild, and severe. Pooled gut samples were collected at 57 d of age to determine viscosities of fore- and hindgut contents. Pooled litter samples were analyzed for moisture, total N, and NH3 production at 14, 28, 42, and 57 d of age. Live performance of birds did not vary among the treatments (P > 0.05). The incidence of pododermatitis was significantly affected by protein source at 42 d (P < 0.05), with birds fed all-vegetable diets showing higher incidence and severity than those fed vegetable plus animal diets. At 57 d of age, birds reared on all-vegetable diets with enzyme supplementation showed a lower incidence of mild footpad lesions compared with other treatments. Enzyme supplementation reduced viscosity of the gut contents irrespective of the protein level or protein source. Higher levels of litter NH3 were observed with high-protein diets (28 and 42 d), all-vegetable diets (28 d), and with enzyme supplementation (28 and 42 d). In this study, enzyme supplementation had little effect on litter total N and NH3 production levels, but reduced viscosity of the gut contents and severity of pododermatitis in older birds.  相似文献   

20.
作者旨在研究大麻籽粕对狄高肉鸡生长发育的影响。选用1日龄狄高肉鸡200只,采用单因素完全随机设计分5组,每组40只,设5个重复,每个重复8只,A组为对照组饲喂基础日粮,B、C、D、E组为试验组,在玉米 豆粕型日粮基础上分3个生理阶段分别添加不同比例的大麻籽粕,部分替换豆粕进行56 d的饲养试验,测定其体重、日增重和料重比。结果:与对照组相比,试验组采食量,累积体重和日增重略高(P>0.05),料重比降低,全期死亡率均低于对照组,结合曲线拟合结果表明:在狄高肉鸡日粮中添加大麻籽粕部分替代豆粕不影响饲料的适口性,且有提高生长速度和饲料转化效率的倾向,在狄高肉鸡饲料中,大麻籽粕替代豆粕的最佳替代比例为Ⅰ期3.8%、Ⅱ期6.4%、Ⅲ期2.5%。  相似文献   

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