Assessing the relationship between fire and grazing on soil characteristics and mite communities in a semi-arid savanna of northern Australia

Northern Australian tropical savannas are subjected to pressures from both grazing and planned and unplanned burning. We know little about the effects of these processes on the below-ground environment. The aim of this study was to investigate the effects of fire, grazing and season on environmental and biological properties of the soil at the base of grass tussocks in a semi-arid savanna rangeland of north Australia. A long-term fire and grazing exclusion experiment was used to test the effects of season, fire and grazing on soil physicochemical factors (soil organic carbon, total nitrogen, ammonium, nitrate levels and bulk density) and soil mite abundance and diversity. Grazed plots were associated with small but significant reductions in total soil nitrogen and organic carbon when compared to 30 year old plots where grazing and fire had been excluded. This suggests slow, long-term losses of nitrogen and soil carbon from an ecosystem with limited available nutrients. Fire had a limited impact on soil properties, but this may reflect the modest experimental fire intensity resulting from fuel reduction due to grazing. Treatment effects on soil bulk density were also negligible. Season had a significant impact on total soil mite abundance and diversity, whereas burning and grazing treatments had no impact on soil mites. Only two morpho-species, one each from the families Cunaxidae and Stigmaeidae, decreased in abundance as a result of grazing. Increased moisture levels in the wet season were associated with increased total nitrogen and the highly mobile nitrate. Changes in mite abundance and diversity reflected these changes in levels of nitrogen and it is possible that increasing total nitrogen availability and soil moisture, is a determinant of mite abundance.     

Surveying soil faunal communities using a direct molecular approach

Soil faunal communities are often phylogenetically diverse and the accurate assessment of the taxonomic structure of these communities is both time-consuming and requires a high level of taxonomic expertise. Here we describe a DNA sequence-based methodology for characterizing soil micro- and mesofaunal communities that is similar to the molecular approaches commonly used to survey soil microbial diversity. The technique involves the direct extraction of faunal DNA from soil, PCR amplification of the extracted DNA with metazoan-specific primers, followed by the construction of clone libraries and direct sequencing of individual PCR products. We used this technique to characterize micro- and mesofaunal community composition from six individual soils representing two land-use types. The technique captured the more abundant faunal groups in the soils (nematodes, Collembola, Acari, tardigrades, enchytraeids) and provided sufficient taxonomic resolution to describe the overall structure of the communities. We compared the results obtained using this molecular approach to results obtained using a traditional, microscopy-based approach and found that the results were broadly similar. However, since biases are inherent in both methods it remains unclear which method provides a more accurate assessment of soil faunal community composition. Although this molecular approach has some distinct disadvantages over the more widely-used direct extraction methods, one advantage is that the taxonomic identification it can provide will be more accurate and consistent across research groups, facilitating effective comparisons of mesofaunal surveys.     

农田土壤螨群落变化与环境因素关系的研究

对中国农业大学实验站农田土壤螨群落变化与环境因素的关系研究结果表明，土壤螨种类与数量随土壤养分的增加而增加，随土壤容重和pH值的增大而减少；长期施用EM堆肥，土壤孔隙多，通气性好，土壤耕层增厚，有机质含量提高，土壤螨种类与数量最多。     

Long-term effects of devegetation on composition and activities (including transcription) of fungal communities of a semi-arid soil

A stable plant cover is essential in the semi-arid soils of the Mediterranean area to maintain their fertility and functionality. In a semi-arid area, we have studied abundance, structure, and presence of active species of fungal communities of a devegetated soil (disturbed soil) and vegetated soil (undisturbed soil). Disturbed soil was covered by small spontaneous vegetation (5–10%) compared to undisturbed soils (70%), and this decreased the content of the total organic C, microbial biomass, microbial activity (adenosine triphosphate), and fungal counts. The composition and activities of fungal communities were also investigated by direct extraction of DNA and RNA from soil. Denaturing gradient gel electrophoresis analysis of 18S ribosomal DNA and 18S ribosomal RNA profiles indicated that total and active fungal communities were changed after vegetation removal.     

The influence of soil properties on the structure of bacterial and fungal communities across land-use types

Land-use change can have significant impacts on soil conditions and microbial communities are likely to respond to these changes. However, such responses are poorly characterized as few studies have examined how specific changes in edaphic characteristics do, or do not, influence the composition of soil bacterial and fungal communities across land-use types. Soil samples were collected from four replicated (n = 3) land-use types (hardwood and pine forests, cultivated and livestock pasture lands) in the southeastern US to assess the effects of land-use change on microbial community structure and distribution. We used quantitative PCR to estimate bacterial–fungal ratios and clone libraries targeting small-subunit rRNA genes to independently characterize the bacterial and fungal communities. Although some soil properties (soil texture and nutrient status) did significantly differ across land-use types, other edaphic factors (e.g., pH) did not vary consistently with land-use. Bacterial–fungal ratios were not significantly different across the land-uses and distinct land-use types did not necessarily harbor distinct soil fungal or bacterial communities. Rather, the composition of bacterial and fungal communities was most strongly correlated with specific soil properties. Soil pH was the best predictor of bacterial community composition across this landscape while fungal community composition was most closely associated with changes in soil nutrient status. Together these results suggest that specific changes in edaphic properties, not necessarily land-use type itself, may best predict shifts in microbial community composition across a given landscape. In addition, our results demonstrate the utility of using sequence-based approaches to concurrently analyze bacterial and fungal communities as such analyses provide detailed phylogenetic information on individual communities and permit the robust assessment of the biogeographical patterns exhibited by soil microbial communities.     

Microbial activity and community structure of a soil after heavy metal contamination in a model forest ecosystem

We assessed the effects of chronic heavy metal (HM) contamination on soil microbial communities in a newly established forest ecosystem. We hypothesized that HM would affect community function and alter the microbial community structure over time and that the effects are more pronounced in combination with acid rain (AR). These hypotheses were tested in a model forest ecosystem consisting of several tree species (Norway spruce, birch, willow, and poplar) maintained in open top chambers. HMs were added to the topsoil as filter dust from a secondary metal smelter and two types of irrigation water acidity (ambient rain vs. acidified rain) were applied during four vegetation periods. HM contamination strongly impacted the microbial biomass (measured with both fumigation-extraction and quantitative lipid biomarker analyses) and community function (measured as basal respiration and soil hydrolase activities) of the soil microbial communities. The most drastic effect was found in the combined treatment of HM and AR, although soil pH and bioavailable HM contents were comparable to those of treatments with HM alone. Analyses of phospholipid fatty acids (PLFAs) and terminal restriction fragment length polymorphisms (T-RFLPs) of PCR-amplified 16S ribosomal DNA showed that HM treatment affected the structure of bacterial communities during the 4-year experimental period. Very likely, this is due to the still large bioavailable HM contents in the HM contaminated topsoils at the end of the experiment.     

Disc harrowing intensity and its impact on soil properties and plant growth of agropastoral systems in the Llanos of Colombia

Oxisols such as those of the Colombian Eastern Plains (Llanos) are susceptible to physical, chemical and biological degradation once brought into cultivation, especially under intensive use of machinery. The main objective of this study was to determine the impact of intensive disc harrowing (2, 4 or 8 disc harrow passes per year over 3 years) on soil physical and chemical properties, soil phosphorus dynamics, plant growth and nutrient acquisition of contrasting agropastoral systems on an Oxisol. The three main systems tested after 2 years of upland rice cultivation were grass-alone pasture (Brachiaria dictyoneura), green manure (Crotalaria juncea), and maize (Zea mays). Native savanna treatment was used as a control. Intensive disc harrowing improved macroporosity values of 0–5 cm soil layer up to 59% for grass-alone pasture system compared to native savanna. Disc harrowing significantly reduced bulk densities for pasture and green manure systems compared to the native savanna in the 0–5 cm soil layer. Intensive disc harrowing significantly improved volumetric moisture content of green manure and maize systems at 5–10 cm soil depth. The distribution of biologically, moderately and sparingly available P, organic P and total P varied under green manure, maize and grass-alone pasture systems. Two passes of disc harrow per year were sufficient for grass-alone pasture, while maize showed greater aboveground production and nutrient acquisition at 8 passes of disc harrow per year. The maize and green manure cropping systems were better than the grass-alone pasture system at separating the effect of increased number of disc harrow passes on soil physical and chemical characteristics.     

Vineyard soil bacterial diversity and composition revealed by 16S rRNA genes: Differentiation by geographic features

Here, we examine soil-borne microbial biogeography as a function of the features that define an American Viticultural Area (AVA), a geographically delimited American wine grape-growing region, defined for its distinguishing features of climate, geology, soils, physical features (topography and water), and elevation. In doing so, we lay a foundation upon which to link the terroir of wine back to the soil-borne microbial communities. The objective of this study is to elucidate the hierarchy of drivers of soil bacterial community structure in wine grape vineyards in Napa Valley, California. We measured differences in the soil bacterial and archaeal community composition and diversity by sequencing the fourth variable region of the small subunit ribosomal RNA gene (16S V4 rDNA). Soil bacterial communities were structured with respect to soil properties and AVA, demonstrating the complexity of soil microbial biogeography at the landscape scale and within the single land-use type. Location and edaphic variables that distinguish AVAs were the strongest explanatory factors for soil microbial community structure. Notably, the relationship with TC and TN of the <53 μm and 53–250 μm soil fractions offers support for the role of bacterial community structure rather than individual taxa on fine soil organic matter content. We reason that AVA, climate, and topography each affect soil microbial communities through their suite of impacts on soil properties. The identification of distinctive soil microbial communities associated with a given AVA lends support to the idea that soil microbial communities form a key in linking wine terroir back to the biotic components of the soil environment, suggesting that the relationship between soil microbial communities and wine terroir should be examined further.     

The positive relationship between soil quality and crop production: A case study on the effect of farm compost application

In order to ensure sustainable agriculture, and for evaluating the effects of management practices on soil processes, tools for assessing soil quality are required. The development and use of a multiparameter index, which includes a wide range of soil properties, have been tested and found useful by several studies. However, soil quality measurements are ‘stand-alone’ tools unless they are either linked to important soil functions, used to characterize (agro)ecosystems or used to predict sustainability or productivity. In our study, the relationship between crop production and soil quality was assessed in a six year old field experiment studying the effect of farm compost (FC) amendment in a crop rotation of potato, fodder beet, forage maize and Brussels sprouts. To justify the hypothesis that repeated FC amendment results in both improved soil quality and consequently higher crop yields, a wide range of chemical, biological and physical soil properties were measured and integrated into a soil quality index (SQI). Next, crop yields were used as a functional goal to verify the causal relationship between SQI and crop production. Our results showed that there were significant changes in chemical, physical and biological soil quality as a result of repeated FC amendment. This was evidenced for example by a remarkable increase in both soil organic carbon (SOC) and total N content. Microbial biomass, the relative amount of bacterivorous nematodes and earthworm number were significantly increased as well and, together with SOC and total N, indicated as the dominant factors in assessing soil quality. The integration of these key indicators into the SQI revealed higher SQI values when FC was applied. In addition, crop yields were increased in all FC treated plots by which SOC was pointed out as the most important indicator influencing crop production. Finally, a causal relationship was observed between soil quality and the yield of potato and fodder beet. We conclude that our SQI may be a promising and useful tool to compare different (soil) management practices in relation to a strategic, regional goal, e.g., sustainable high yields. Before generalizing, we recommend a thorough validation of our SQI in other long-term field experiments.     

A comparison between continuous and controlled grazing on a red duplex soil. II. Subsequent effects on seedbed conditions, crop establishment and growth

The effects of past grazing management practice on subsequent seedbed condition, draft requirements, fuel consumption, crop establishment and growth, and grain yield and quality were examined using three tillage systems on two sowing dates. The crop was wheat (Triticum aestivum), sown on a fragile sandy clay loam (red duplex soil) in a dryland agricultural area (307 mm average annual rainfall) of Western Australia. The three tillage-sowing systems investigated were: (i) scarifying followed by sowing with wide (180 mm) points; (ii) direct drilling with wide (180 mm) points; (iii) direct drilling with narrow (50 mm) inverted ‘T’-shaped Super-Seeder points. The two sowing dates provided differences in seedbed water content at sowing time. The three grazing management strategies practised in the previous pasture year were: (i) traditional set-stocking (where sheep were grazed continuously for 17 weeks, beginning soon after the start of the early winter rains); (ii) controlled grazing (where sheep were temporarily removed from the enclosure when the topsoil was close to its plastic limit); (iii) no grazing (where the pasture was mown to simulate grazing without trampling).Tillage prior to sowing with wide points reduced the mechanical impedance of the soil following set-stocking and provided a good seedbed for successful crop establishment and growth. In both the controlled-grazing management treatment and the treatment where the pasture had been mown the soil was suitable for direct drilling with both wide and narrow points (i.e. no pre-sowing tillage was required). The use of narrow points had the added advantage of requiring less fuel, but the need for a suitable implement to cover seeds was more critical than for wider sowing points. There were no advantages with respect to grain yield from adopting a controlled-grazing management practice owing to the lack of finishing rainfall. However, grain protein levels were higher in both the controlled and ungrazed treatments compared with the set-stocking treatment.     

Common and distinguishing features of the bacterial and fungal communities in biological soil crusts and shrub root zone soils

Soil microbial communities in dryland ecosystems play important roles as root associates of the widely spaced plants and as the dominant members of biological soil crusts (biocrusts) colonizing the plant interspaces. We employed rRNA gene sequencing (bacterial 16S/fungal large subunit) and shotgun metagenomic sequencing to compare the microbial communities inhabiting the root zones of the dominant shrub, Larrea tridentata (creosote bush), and the interspace biocrusts in a Mojave desert shrubland within the Nevada Free Air CO₂ Enrichment (FACE) experiment. Most of the numerically abundant bacteria and fungi were present in both the biocrusts and root zones, although the proportional abundance of those members differed significantly between habitats. Biocrust bacteria were predominantly Cyanobacteria while root zones harbored significantly more Actinobacteria and Proteobacteria. Pezizomycetes fungi dominated the biocrusts while Dothideomycetes were highest in root zones. Functional gene abundances in metagenome sequence datasets reflected the taxonomic differences noted in the 16S rRNA datasets. For example, functional categories related to photosynthesis, circadian clock proteins, and heterocyst-associated genes were enriched in the biocrusts, where populations of Cyanobacteria were larger. Genes related to potassium metabolism were also more abundant in the biocrusts, suggesting differences in nutrient cycling between biocrusts and root zones. Finally, ten years of elevated atmospheric CO₂ did not result in large shifts in taxonomic composition of the bacterial or fungal communities or the functional gene inventories in the shotgun metagenomes.     

Effects of filter type and extraction efficiency on nitrogen mineralization measurements using the aerobic leaching soil incubation method

Aerobic incubation of soils with sequential leachings to extract mineralized N is often used to determine N mineralization potential and N availability in the laboratory. This study used tropical forest soils with differing mineralogy and texture to address: (1) the effects of filter type and equilibration time on soil moisture and N mineralization and (2) the N extraction efficiency of 0.01 M CaCl₂, minus-N nutrient solution (containing 0.004 M CaCl₂) and 2 M KCl. Use of glass microfiber filters compared to cellulose acetate or polyethersulfone membrane filters resulted in a lower moisture content for both low-and high-clay soils. However, filter type did not affect N mineralization. Under 47 kPa suction, soil moisture equilibration occurred between 240 and 360 min regardless of filter type. Extraction efficiency for mineralized N using 0.01 M CaCl₂ or minus-N nutrient solution was lower in forest soils of smectitic mineralogy and soils with a higher proportion of macroaggregates. However, with the exception of allophanic soils, the cumulative amount of N mineralized measured in a long-term incubation for approximately 1 year was not different when either a leaching or an unleached incubation method was used. These results indicate that researchers may wish to conduct preliminary evaluations to determine whether their incubation method will achieve a desired uniform moisture level and N extraction efficiency.     

Thermal shock and rain effects on soil surface characteristics: A laboratory approach

In addition to its direct impact on soil physical and chemical soil properties, fire produces a sudden change in plant cover. The post-fire impact of falling raindrops on unprotected soil surfaces is a major cause of detachment of aggregates, physical degradation and erosion of soils. The aim of this work was to analyse the effects of burning intensity and rain under factor-controlled conditions using unaltered soil samples. Assessed variables were soil organic carbon, aggregate stability and water repellency (0–1 cm mineral soil), as well as soil surface compaction and hydraulic conductivity. Unaltered topsoil cores were obtained in a mature Mediterranean gorse shrubland. We applied two successive treatments: burning (unburned, low and high burning levels) and rain (77 mm/h). The soil properties studied were scarcely affected by burning. However, soils showed high vulnerability to raindrop impact: a) aggregate stability and organic carbon were not significantly affected by burning; b) low intensity burning increased the frequency of samples with moderate water repellency, whereas unburned together with high burning showed more cases of low and extreme water repellency; c) the rain treatment produced a significant decrease in hydraulic conductivity although this response was independent of burning level; d) the highest reduction in hydraulic conductivity was observed in the samples with highest values prior to the rain treatment, and this was related to the highest organic carbon contents, and e) the reduction in hydraulic conductivity could be explained by the development of a thin and friable surface crust, although the cone penetrometer was not sensitive enough to detect this observed phenomenon.     

土壤养分与春小麦产量关系及最佳施肥量研究

土壤养分与春小麦产量关系及最佳施肥量研究结果表明 ,春小麦产量与土壤养分关系十分密切 ,尤其与土壤有机质、碱解氮含量相关性较好 ,而与速效磷和速效钾关系较差 ;土壤养分供N量严重不足、部分缺P但富K ,这与产量 养分敏感性分析结论相一致 ;春小麦最佳施N量较常规减少 10 % ,施P量减少 15 %。     

Positive relationship between herbaceous layer diversity and the performance of soil biota in a temperate forest

The current decline in biodiversity is particularly pronounced in the herbaceous layer of forest ecosystems. We explored the relationship between a naturally occurring plant diversity gradient in the understory vegetation of a deciduous forest and several above-and belowground ecosystem processes. We show that particularly soil microbial parameters and microarthropod densities are positively correlated with plant species richness. These results confirm recent findings in grassland ecosystems and highlight the intimate interconnectance between the diversity and functioning of above-and belowground compartments. We conclude that irrespective of a potential causal relationship between plant species richness and belowground processes, it is essential to consider the performance of soil biota in order to understand the relationship between herbaceous layer composition and ecosystem function.     

Temporal change in land use and its relationship to slope degree and soil type in a small catchment on the Loess Plateau of China

We sought to detect the temporal change (1958–1999) in land use patterns and its relationship to physical landscape parameters in a small catchment in the semi-arid hilly area of the Chinese Loess Plateau. Degree of slope and soil type were selected as stable discriminating parameters that might constrain land use. With the help of GIS and canonical correspondence analysis, the relationship between rural land use distribution or transformation and the selected physical parameters was examined. The land use had undergone a general shift from farmland to woodland or grassland. Canonical correspondence analyses (CCA) indicated that a relationship between land use and the selected physical parameters was evident, farmland coincided with favorable conditions of fertile soil and gentle slope, while grassland and woodland were associated with conditions of poorer soil and steep slope. In the more favorable conditions the main land use change process was the intensification of farming, while in the less favourable conditions it was the abandonment of farmland. A thorough understanding of the relationship between land use temporal or spatial patterns and landscape physical parameters in the Loess Plateau of China, like degree of slope and soil type, will enhance our capability to predict landscape dynamics and lead to more sound and effective land use management strategies.     

省域土壤有机碳空间分布的主控因子——土壤类型与土地利用比较

基于河北省第二次全国土壤普查数据,运用方差分析和回归分析对比了河北省土壤类型和一级土地利用类型对0～20 cm深土壤有机碳空间分布的影响,探讨了省域土壤有机碳空间分布的主控因子。研究结果表明,土壤类型和土地利用是河北省表层土壤有机碳密度空间分布的重要影响因子。其中土壤类型对土壤有机碳密度空间分布的影响与土壤分类级别相关,土壤分类级别越低,对土壤有机碳密度空间变异的反映能力越大。与土壤类型相比,土地利用对表层土壤有机碳密度空间分异的解释能力要大于土类,但小于亚类和土属。为此,在省域尺度对土壤有机碳密度进行区域预测和估算时应将土地利用和土壤类型结合起来作为土壤有机碳空间分布的主控因子,优先考虑土地利用后,在相同土地利用类型内再尽量以低级土壤分类进行空间预测或估算。     

植被叶面积指数与覆盖度定量表征红壤区土壤侵蚀关系的对比研究

选择较合理的植被指标,对提高植被水土保持效益评价精度具有重要理论与现实意义。本研究基于福建省长汀县河田镇12个土壤侵蚀试验小区2007年和2008年2 a的降雨、径流、泥沙数据及各小区的植被叶面积指数(LAI)、植被覆盖度(VFC)资料,利用对比与统计分析方法,研究了2种植被覆盖类型(纯马尾松林、马尾松林草)和不同覆盖度(80%、60%、45%、30%、15%和5%)的红壤水土流失特点,探讨了LAI与VFC定量表征水土流失关系的稳定性和可靠性。结果表明,与裸地小区比较,马尾松纯林覆盖一定程度上降低了土壤侵蚀模数,对径流的减少作用并不明显;而马尾松林草覆盖可显著减少25%左右的径流,土壤侵蚀模数减少也在90%以上,林草结合的植被覆盖结构具有更强的水土保持功能。以LAI表征2种植被覆盖类型的土壤侵蚀模数,均能达到显著水平(p0.05);而以VFC来表征土壤侵蚀模数,仅马尾松林草覆盖类型达到了显著水平。选择LAI表征土壤侵蚀模数来评价植被水土保持效益,将更为稳定和可靠。     

Differences in the activity and bacterial community structure of drained grassland and forest peat soils

The microbial activity and bacterial community structure were investigated in two types of peat soil in a temperate marsh. The first, a drained grassland fen soil, has a neutral pH with partially degraded peat in the upper oxic soil horizons (16% soil organic carbon). The second, a bog soil, was sampled in a swampy forest and has a very high soil organic carbon content (45%), a low pH (4.5), and has occasional anoxic conditions in the upper soil horizons due to the high water table level. The microbial activity in the two soils was measured as the basal and substrate-induced respiration (SIR). Unexpectedly, the SIR (μl CO₂ g⁻¹ dry soil) was higher in the bog than in the fen soil, but lower when CO₂ production was expressed per volume of soil. This may be explained by the notable difference in the bulk densities of the two soils. The bacterial communities were assessed by terminal restriction fragment length polymorphism (T-RFLP) profiling of 16S rRNA genes and indicated differences between the two soils. The differences were determined by the soil characteristics rather than the season in which the soil was sampled. The 16S rRNA gene libraries, constructed from the two soils, revealed high proportions of sequences assigned to the Acidobacteria phylum. Each library contained a distinct set of phylogenetic subgroups of this important group of bacteria.