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大豆疫霉病的症状与分离技术 总被引:5,自引:0,他引:5
大豆疫霉病的症状与分离技术李宝英(黑龙江省农科院合江农科所佳木斯154007)大豆疫霉病Phytophthoramegaspermaf.sp.glycineaKuan&Erwin也叫大豆疫霉根腐病,是一种分布较广、危害极其严重的土传性病害。产量损失一... 相似文献
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2003年3月7~12日,广州局新沙办事处从一批美国进口的58,782t大豆中检出一类危险性病害大豆疫霉菌(Phytophthora sojae Kaufmann & Gerdemann). 相似文献
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从日本进境大豆种子中检出大豆疫病菌 总被引:4,自引:1,他引:3
从日本进境大豆种子中检出大豆疫病菌高秀兰种焱李文利陈洪俊(北京动植物检疫局100029)1997年1月7日,在国际邮件交换局截留的1袋大豆种子中(4个品种,710g),检查出了大豆疫病菌Phytophthoramegaperma(Drechs.)f.... 相似文献
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大豆疫病是大豆生产中的毁灭性病害之一 ,自1 948年在美国首次报道该病以来 ,已先后在美洲、欧洲、日本和澳大利亚等 2 0多个国家发生。目前该病引起的根腐和茎腐病已成为美国大豆较为普遍的病害 ,其造成的损失也是巨大的 ,如 1 989~ 1 991年间仅在美国中北部 ,在使用抗病品种的情况下 ,平均每年造成的损失仍达 93万 t,价值 1 .87亿美元。 2 0 0 2年 3月 ,防城港某公司从美国 RESERVE港进口大豆 5 8443 .2 1 9t,经我局检验检疫人员抽样鉴定出一种我国禁止进境的危险性病菌——大豆疫病菌 Phytophthora sojae Kaufmann & Gerdemann,… 相似文献
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2005年1月,张家港局对一批来自美国的6万t大豆实施检疫时,抽取了样品和其中携带的土壤颗粒,经过PCR快速检测方法和传统的叶碟诱集法的两次相互验证检测,确认该批大豆中携带大豆疫霉病菌.现已对该批大豆进行了除害处理. 相似文献
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一种适用于大豆疫霉菌研究的培养基 总被引:1,自引:0,他引:1
1989年沈崇尧和苏彦纯首次在我国分离到大豆疫霉菌(Phytophthora sojae M.J.Kaufmann&J.W.Gerdemann)。目前,该菌已成为三江平原大豆产区影响大豆生产的重要病原菌。在国外,用于P.sojae研究的主要培养基之一是利马豆(Phaseolus lunatus L.,Lima bean)培养基,而国内利马豆很少,一般不易获得,为此,我们尝试用与利马豆同属的粒用菜豆(芸豆)(P.vulgaris L.,Common bean)制作培养基代替利马豆培养基用于大豆疫霉菌的研究。 相似文献
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P. J. Oyarzun 《European journal of plant pathology / European Foundation for Plant Pathology》1993,99(2):61-75
Infection of pea roots by soil-borne pathogens causes foot and root rot. In 1985 research was started to develop a method to predict the root rot likely to occur in prospective pea fields. In a bioassay the pea cultivar Finale was sown in a composite soil sample from each field in pots under standardized conditions in the greenhouse. The plants were removed at the green bud stage and the severity of root rot recorded. Between 1985 and 1988 approximately 200 field pea crops were monitored for root rot development. Forty-eight fields were bioassayed in 1986, 51 in 1987 and 30 in 1988. Each year, root rot readings in the bioassay and disease severity readings at field sampled plants at flowering and green pod were linearly correlated (P<0.001). As the degree of root rot in the field crop increased, there was a proportional lower yield. In heavily infested fields, up to a 50% yield reduction occurred.The bioassay in pots proved to be a reliable method for predicting root rot severity in sampled pea fields. 相似文献
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Phytophthora root rot of sweet pepper 总被引:1,自引:0,他引:1
N. A. M. Van Steekelenburg 《European journal of plant pathology / European Foundation for Plant Pathology》1980,86(5):259-264
Phytophthora capsici proved to be the causal agent of a root and crown rot of sweet pepper in the Netherlands.P. capsici was pathogenic on sweet pepper, tomato and sometimes on eggplant but not on tobacco Xanthi. Of these test plants only tomato was infected byP. nicotianae.No different symptoms in plants infected with eitherP. capsici orP. nicotianae were found. Dipping the roots of tomato and sweet pepper plants in a suspension ofP. capsici resulted in a more severe attack than pouring the suspension on the stem base.Resistance in tomato toP. nicotianae did not include resistance toP. capsici. A method to distinguishP. capsici fromP. nicotianae after isolation from soil is described. Both species were able to infect green fruits of tomato and sweet pepper.p. capsici survived in moist soil in the absence of a host for at least 15 months.Samenvatting
Phytophthora capsici bleek de oorzaak te zijn van een voet-en wortelrot in paprika op twee bedrijven in 1977 in Nederland.P. capsici was pathogeen op paprika, tomaat en soms op aubergine maar niet op tabak Xanthi.P. nicotianae tastte van deze toetsplanten alleen tomaat aan. Verschillen in symptomen tussenP. nicotianae enP. capsici werden bij tomaat niet waargenomen.Het dompelen van de wortels in eenP. capsici suspensie gaf een ernstiger aantasting dan het begieten van de wortelhals met deze suspensie.Resistentie in tomaat tegenP. nicotianae bleek geen resistentie tegenP. capsici in te houden.
P. capsici kan in grond worden aangetoond door groene paprikavruchten als vangsubstraat te gebruiken.P. capsici enP. nicotianae kunnen beide zowel vruchten van tomaat als paprika aantasten.
P. capsici overleefde een periode van 15 maan den in vochtige grond waarop geen waardplant werd geteeld. 相似文献
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J. H. Hellinga J. J. Bouwman K. Scholte J. J. s'Jacob 《European journal of plant pathology / European Foundation for Plant Pathology》1983,89(5):229-237
In an outdoor pot trial the causes of root rot im maize were studied with a nematicide (oxamyl) and fungicides (captafol and metalaxyl). Root rot is particularly severe with continuous cropping of maize on sandy soil. There was clear evidence ofPythium spp. being the main, cause of it.Fusarium spp. may contribute to root rot at a later stage in the growth of the crop. The parasitic root nematodesPratylenchus crenatus andTylenchorhynchus dubius were harmless in the present trial. Application of metalaxyl significantly increased the population ofT. dubius. No interaction was found between fungicides and a nematicide. 相似文献
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Observations on Pythium root rot of wheat and barley 总被引:1,自引:1,他引:0
J. M. WALLER 《Plant pathology》1979,28(1):17-24
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ABSTRACT Cylindrocarpon root rot, caused by Cylindrocarpon destructans, is an important disease on ginseng (Panax quinquefolius) in Canada. We studied the effects of iron (Fe) on disease severity and pathogen growth. When Hoagland's solution was amended with Fe at 56 and 112 mug/ml compared with 0 mug/ml, disease initiation and final severity on hydroponically maintained ginseng roots was significantly (P<0.0001) enhanced. Under field conditions, wounding of roots with a fine needle followed by application of 0.05% FeNaEDTA to the rhizosphere of treated plants significantly enhanced Cylindrocarpon root rot in 2003 and 2004 compared with unwounded roots with Fe or wounded roots without Fe. Foliar applications of Fe (as FeNaEDTA) to ginseng plants three times during the 2002 and 2003 growing seasons significantly increased Fe levels in root tissues. These roots developed larger lesions following inoculation with C. destructans in vitro. When radioactive Fe ((59)Fe) was applied to the foliage of ginseng plants, it was detected in the secondary phloem and in cortical and epidermal tissues within 1 week. Artificially wounded areas on the roots accumulated more (59)Fe than healthy areas. Diseased tissue also had threefold higher levels of phenolic compounds and Fe compared with adjoining healthy tissues. High-performance liquid chromatography analysis revealed enhanced levels of protocatechuic acid, chlorogenic acid, caffeic acid, ferulic acid, cinnamic acid, phloridizin, and quercetin. Phenolic compounds produced in diseased and wounded tissues sequestered Fe in vitro. The effects of Fe on mycelial growth, conidial germ tube length, and secondary branching of germ tubes of C. destructans were examined in vitro. When grown on Chrome-azurol S medium, Fe also was sequestered by C. destructans through siderophore production, which was visualized as a clearing pigmented zone at the margin of colonies. Mycelial dry weight was significantly increased in glucose/ yeast broth containing Fe at 56 or 112 mug/ml. Conidial germ tube length and secondary branching of hyphae also were enhanced after 8 and 16 h by Fe. Colony growth of C. destructans was not enhanced by Fe, but significantly greater spore production was observed with Fe at 56 and 112 mug/ml compared with no Fe in the medium. Although these levels of Fe had no effect on fungal pectinase enzyme activity, polyphenoloxidase (PPO) activity was significantly (P <0.0001) enhanced. We conclude that Fe enhances Cylindrocarpon root rot through enhanced pathogen growth, sporulation, and PPO enzyme activity. Fe sequestered by phenolic compounds produced in wounded tissues can enhance Fe levels at the site of infection. The pathogen also has the ability to sequester Fe at these sites. 相似文献
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