多效唑对盐胁迫下高羊茅耐盐性的影响

应用盆栽控盐的方法,研究施用不同浓度(200mg/L、400mg/L、600mg/L)的多效唑对盐处理(0.8%NaCl)高羊茅草坪草耐盐性的影响。结果表明,高羊茅在盐胁迫下表现出根长降低、生物量减少、相对含水量减少、丙二醛含量增加等一系列变化。施用不同浓度多效唑后,能增加高羊茅生物量,提高根长,促进叶片可溶性糖的积累,保持更高的叶片相对含水量,减少丙二醛的生成,从而在一定程度上缓解了高羊茅的盐害。400mg/L多效唑对增加高羊茅耐盐性的效果最理想。     

多效唑对盐胁迫下高羊茅耐盐性的作用

应用盆栽控盐方法,研究施用不同质量浓度的多效唑(200、400和600 mg/L)对盐处理(0.8%NaCl)高羊茅Festuca arundinacea草坪草耐盐性的影响。结果表明,高羊茅在盐胁迫下表现出根长缩短、生物量减少、相对含水量减少、丙二醛含量增加等一系列变化。施用不同质量浓度多效唑后,能不同程度地增加高羊茅生物量,延长根长,促进叶片可溶性糖的积累,保持更高的叶片相对含水量,减少丙二醛的生成,从而在一定程度上缓解了高羊茅的盐害。在所有的处理中,质量浓度为400 mg/L的多效唑对提高高羊茅耐盐性的效果最佳。     

三种生长调节剂对高羊茅生长特性及赤霉素含量的影响

通过盆栽方法测定植物生长调节剂对高羊茅(Festuca arundinacea Schreb.)草坪草株高、叶宽、叶绿素含量及赤霉素含量的影响.施用多效唑、矮必施和乙烯利3种生长调节剂,各设3个浓度梯度,分别为多效唑150 mg/L、200 mg/L、250 mg/L,乙烯利1000 mg/L1、200 mg/L、1400 mg/L,矮必施200 mg/L2、50 mg/L3、00 mg/L.结果表明:施用多效唑、矮必施和乙烯利降低了草坪高度,其中以250 mg/L多效唑、1200 mg/L乙烯利和300 mg/L矮必施效果最好;3种植物生长调节剂均能对高羊茅叶片的宽度产生影响,多效唑和矮必施促使叶片变窄,而乙烯利初期使叶片变宽,后期变宽效果消失;多效唑和乙烯利都不会对高羊茅草坪的颜色产生影响,矮必施在施药后前2个星期使叶色变深,第3星期后影响逐渐消失;多效唑、乙烯利和矮必施均能促使高羊茅体内赤霉素的含量降低,降低幅度达到30.6%~64.0%.综合考虑,高羊茅草坪施用250 mg/L浓度的多效唑时效果最好.     

多效唑对2种冷季型草坪草生长的影响

利用室内盆栽试验,研究不同浓度（100、 200、 300、 400、 500 mg/L）的多效唑对2种冷季型草坪草：草地早熟禾Poa pratensis--巴林和高羊茅Festuca arundinacea--法恩的株高、倒2叶长度、叶片内叶绿素含量、丙二醛含量和过氧化氢酶活性的影响。结果表明:不同浓度的多效唑在降低2种冷季型草坪草的株高和缩短叶片长度的同时,能显著增加2种草坪草的叶绿素含量,并使其组织中丙二醛含量降低和过氧化氢酶活性增强,且这种作用随浓度的升高而呈增加的趋势,说明多效唑可以提高草坪草的抗逆性。     

植物生长调节剂对高羊茅草坪质量的影响

探讨了喷施不同浓度的矮壮素和一定浓度的多效唑对建植五年的高羊茅草坪质量的影响。结果表明,施用矮壮素可延缓高羊茅的生长速度,随着浓度的增加延缓作用加强,高羊茅的地下生物量及抗病性随矮壮素浓度增加而提高,低、中浓度的矮壮素改善了高羊茅的颜色、整齐度,随着浓度增加,这种作用由促进转为抑制,矮壮素以200μg/g浓度为宜;施用多效唑可延缓高羊茅的生长速度,促进地下生物量积累,增强抗病性,但抑制分蘖,对颜色、整齐度有伤害作用。     

多效唑在高羊茅草坪中的应用

在高羊茅Festuca arundinacea生长的旺盛季节,分别进行不同浓度的多效唑处理,研究多效唑对高羊茅生长的影响。试验结果表明:多效唑能使高羊茅叶绿素含量明显增加,节间显著缩短,茎增粗;高羊茅的株高随多效唑浓度的增加而降低;叶片大小也随多效唑浓度的增加而减小;多效唑处理高羊茅具有显著的抑制生长和改善绿化景观效果的作用。处理浓度以600~800 mg/kg为宜。     

植物生长调节剂对高羊茅草坪质量的影响

探讨了喷施不同浓度的矮壮素和一定浓度的多效唑对建植五年的高羊茅草坪质量的影响。结果表明,施用矮壮素可延缓高羊茅的生长速度,随着浓度的增加延缓作用加强,高羊茅的地下生物量及抗病性随矮壮素浓度增加而提高,低、中浓度的矮壮素改善了高羊茅的颜色、整齐度,随着浓度增加,这种作用由促进转为抑制,矮壮素以２００μｇ／ｇ浓度为宜;施用多效唑可延缓高羊茅的生长速度,促进地下生物量积累,增强抗病性,但抑制分蘖,对颜色     

多效唑对高羊茅草坪草生长特性的影响

采用了不同浓度的多效唑（0．8、1．0、1．3mg/g)对高羊茅草坪进行叶面喷施，以研究多效唑对高羊茅生长特性的影响。结果表明：3种不同浓度的多效唑处理均表现出显著抑制高羊茅草坪草地上部分的伸长生长的效果，1．3mg/g的多效唑处理使高羊茅生长速度和修剪量明显降低1．0mg/g的多效唑使高羊茅草坪草的密度显著增加，明显促进高羊茅的分蘖，却使高羊茅叶绿素含量明显降低。     

春施多效唑对高羊茅生长及饲草品质影响

春施不同浓度的多效唑对高羊茅Festuca arundinacea的生长及饲草品质影响的研究结果表明,多效唑对高羊茅的营养生长具有显著的抑制作用,多效唑处理后30 d,处理浓度为250,500,750和1 000 mg/L时,株高分别比对照降低了37.9%,43.5%,62.6%和71.1%.多效唑处理后叶色加深,叶片变小,叶面积指数下降,光合有效面积减小,地上部干物质积累量减少,与此同时单株茎蘖数增加,叶茎比提高,饲草中粗蛋白含量增加,中性洗涤纤维含量下降,饲草品质得到了改善.     

不同盐生境下硅对高羊茅生物量及生理生化特征的影响

利用水培实验研究了不同盐生境下硅对高羊茅幼苗生物量及生理生化特征的影响。研究结果表明,高羊茅幼苗生物量随着盐浓度增加而逐渐降低,200 mmol/L的盐浓度为高羊茅幼苗的临界盐浓度,盐浓度小于该临界值时,添加硅显著增加了高羊茅幼苗生物量;盐浓度大于该临界值时,硅对高羊茅幼苗生物量没有明显影响,说明硅调节盐生境下高羊茅的生长能力与环境内的盐浓度密切相关。在盐浓度临界值之内,水培条件下硅通过增加盐胁迫下高羊茅幼苗体内SOD、CAT、POD活性,降低盐胁迫条件下高羊茅幼苗体内的丙二醛、脯氨酸、可溶性糖含量和相对电导率,其中盐浓度为100 mmol/L时,丙二醛含量降幅最大,为18.05%,当盐浓度为50 mmol/L时,脯氨酸含量降幅最大,为23.63%。这不仅说明了硅可增强高羊茅幼苗适应盐生境的能力,而且证明了硅直接参与了盐胁迫条件下高羊茅的生理生化过程。     

癸氧喹酯干混悬剂含量测定的改进

采用高效液相色谱法测定癸氧喹酯干混悬剂的含量,在2-250μg/mL范围内,峰面积的常用对数与进样量浓度的常用对数呈良好的线性关系,R^2=1(n=5),平均回收率为99.24%~99.51%,RSD在0.05%~0.28%。此方法分析时间短,样品前处理简便、定量结果准确,重现性好,结果满意,为其质量控制提供了依据。     

商会自治的基石——商会自治规范研究

在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。     

猪毛色遗传的研究进展

本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。     

中华人民共和国农业部公告 第267号

为贯彻落实《兽药生产质量管理规范》(简称《兽药GMP》),进一步推动兽药GMP实施进程,我部制定了《兽药生产质量管理规范检查验收办法》,现予公告。本公告自2003年6月1日起施行。附件:兽药生产质量管理规范检查验收办法二○○三年四月十日第一章 总则 第一条 为推动《兽药生产质量管理规范》(以下简称兽药GMP)的实施,规范兽药GMP检查验收工作,制定本办法。 第二条 农业部负责全国兽药GMP管理和检查验收工作;负责制修订兽药GMP检查验收管理规定;负责兽药GMP检查员队伍建设和监督管理工作,负责国际兽药贸易中GMP互认工作。 …     

鸡败血支原体垂直传播模式及其阻断方法

以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。     

Comparison of 2 methods of centesis of the bursa of the biceps brachii tendon of horses

REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air.     

不同样本商品蜂蜜中硒含量的测定

用硝酸和高氯酸消化蜂蜜,使硒游离出来,在微酸性环境下,硒和2,3-二氨基萘(DAN)生成有较强荧光的物质,用环己烷萃取,在激发波长378nm,荧光波长518nm处测定其荧光强度。蜂蜜中硒含量范围:0.10～0.82μg/g。表明:蜂蜜应视为天然富硒营养品。     

乳酸杆菌益生作用机制的研究进展

乳酸杆菌作为益生菌广泛用于人和动物。本文综述了乳酸杆菌改善宿主健康的机制。乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道。文章重点讨论了乳酸杆菌表面成分（表面蛋白、脂磷壁酸和肽聚糖）与肠道受体（Ｃ型凝集素受体、Ｔｏｌｌ样受体和 Ｎｏｄ样受体）,阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制。     

Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF

Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo.