Pheromone-mediated mating disruption in the millet stem borer, Coniesta ignefusalis (Lepidoptera: Pyralidae)

The millet stem borer, Coniesta ignefusalis Hampson (Lepidoptera: Pyralidae), is a major pest of pearl millet in the Sahelian region of Africa. The female sex pheromone has been identified and synthesised, and previous research had shown that the synthetic pheromone could cause high levels of reproductive communication disruption in small plots when released at rates of 640 mg/ha/day, using PVC resin formulation renewed every seven days to maintain efficiency. In the present research, in experiments in farmers’ fields in Niger, 86.8% (SE = 2.6%) communication disruption was achieved when polyethylene vials loaded with 0.5 mg pheromone at 400 dispensers/ha were used and replaced every 21 days. Polyethylene vials loaded with 80 mg pheromone gave uniform, zero-order release at approximately 0.05 mg/day at 27 °C. Experiments carried out on replicated 0.5 ha plots in farmers’ fields in Niger using a single application of these dispensers at 400 dispensers/ha resulted in at least 99% suppression of pheromone trap catches of male C. ignefusalis moths in treated plots relative to numbers in untreated plots for up to 3 months. However, sampling the central portions of these plots before and after harvest showed no significant differences in infestation, damage or yield loss between plots treated with pheromone and untreated plots. This may have been because of small plot size and the immigration of mated female moths into the treated plots which negated any reduction of mating of females within the treated plots. Comparisons of numbers of male C. ignefusalis moths in traps baited with the standard 0.5 mg monitoring lures and those baited with the 80 mg disruption dispensers showed catches in the latter were only 10-20% of those in the former; indicating high level communication disruptions in traps with high dose dispensers. Implications of using insect synthetic pheromones in the development of integrated management of C. ignefusalis in pearl millet cropping systems in the Sahel are discussed.     

Monitoring and action threshold determination for the obscure mealybug Pseudococcus viburni (Signoret) (Hemiptera: Pseudococcidae) using pheromone-baited traps

The absence of effective pest monitoring has resulted in an increase in populations of the obscure mealybug Pseudococcus viburni (Signoret) (Hemiptera: Pseudococcidae) in pome fruit orchards in the Western Cape Province of South Africa recently. The sex pheromone for P. viburni was recently identified and synthesized. Flight activity of adult male P. viburni was monitored by placing and servicing three, evenly spaced pheromone-baited traps per orchard in three pome fruit growing regions used for the study. Fortnightly fruit sampling was done by randomly picking three fruits per tree for the duration of each fruit season, dissecting and noting mealybug infestation. The biweekly male P. viburni trap catch information and fruit infestation data collected over two seasons were correlated. There was a positive and significant relationship between the fruit infestation and number of P. viburni adult males caught in pheromone-baited traps (r² = 0.454, P < 0.001). The action threshold level was estimated to be 2.5 male P. viburni caught per trap per fortnight at an economic threshold of 2% fruit infestation. This monitoring method was less labor intensive, more accurate and quicker than the current visual sampling and monitoring techniques.     

Calendar and degree-day requirements for emergence of adult Macroglenes penetrans (Kirby), an egg-larval parasitoid of wheat midge, Sitodiplosis mosellana (Géhin)

The chalcidoid wasp, Macroglenes penetrans (Kirby), is an important parasitoid of wheat midge, Sitodiplosis mosellana (Géhin), in Europe and western Canada. Emergence of adult wasps was evaluated at 12 sites in Saskatchewan in 1991-2000. Emergence was assessed in relation to calendar days and accumulated degree-days above five different threshold air temperatures. Male wasps emerged 1-2 days before the female wasps. Dates for 10%, 50% and 90% emergence of both wasp sexes were July 16, July 21 and July 30, respectively. Standard deviations (SD) indicated that emergence dates varied by 6.2-7.7 days. The variation in dates related to degree-day accumulations between March 1 and July 31. Wasps emerged 2-12 days earlier than expected at sites with the highest accumulation and 2-17 days later than expected at sites with the lowest accumulation. Accumulated degree-days above 9 °C provided the most accurate estimate of 10% emergence (450 DD, SD = 2.4 days) and 90% emergence (579 DD, SD = 5.0 days). Accumulated degree-days above 5 or 9 °C provided the most accurate estimate of 50% emergence (823 DD, SD = 3.0 days; 503 DD, SD = 3.1 days, respectively). Deviations between observed and expected emergence were greatest at sites with either low or high precipitation. In most instances, wasps emerged 1-8 days earlier than expected at sites that received 20-40 mm rain in May and 1-11 days later than expected at sites that received more than 145 mm rain in May and June. Degree-days above 5 or 9 °C minimized the variation at these sites. Emergence was re-assessed in 2008 and 2009. In both years, accumulated degree-days above 9 °C predicted 10%, 50% and 90% emergence within 0.1-2.2 days. Forecast maps, based on daily degree-day accumulations above 9 °C, would assist producers in monitoring their fields for the presence of wasps. Producers could also adjust the timing, rate and placement of sprays for control of wheat midge to protect and conserve the parasitoid.     

A pheromone trap monitoring system for the saddle gall midge,Haplodiplosis marginata (von Roser) (Diptera: Cecidomyiidae)

Outbreaks of saddle gall midge, Haplodiplosis marginata (von Roser) (Diptera: Cecidomyiidae) have been reported in Belgium and other European countries since 2010. Because of the sporadic nature of this pest, which can sometimes be very harmful to cereal crops, an effective monitoring tool is required, both to determine the optimal timing for insecticide applications, and to understand the enigmatic population dynamics of this insect. Following the recent identification of the major sex pheromone component of the saddle gall midge, non-2-yl butanoate, a slow-release dispenser was developed using rubber septa. The release rates of 5 mg and 10 mg-loaded dispensers were initially measured under laboratory conditions, and their effectiveness in terms of pheromone loading and use duration was assessed in the field. The experiments showed that sticky traps baited with 5 mg pheromone-loaded rubber dispensers, renewed every 6 weeks, are suitable for accurately monitoring male H. marginata flights.     

Relative toxicity of insecticides to the crucifer pests Plutella xylostella and Myzus persicae and their natural enemies

The widespread and intensive use of conventional pesticides, particularly insecticides, presents a major risk to natural enemies of target pests, as well as to the environment in general. The aim of this study was to investigate the differential intrinsic toxicity of insecticides to two key pests of crucifers, Plutella xylostella and Myzus persicae and their respective hymenopteran parasitoids, Cotesia vestalis and Aphidius colemani. Such knowledge can help inform effective integration of insecticides and biological control in IPM systems. Three insecticides generally regarded as being compatible with natural enemies (abamectin, spinosad and indoxacarb) and one compound regarded as harmful to natural enemies (lambda-cyhalothrin) were examined. A comparative measure of the intrinsic toxicity of fresh deposits of insecticides on Chinese cabbage leaf discs was determined for both pest and parasitoids species after exposure to insecticide for 24 h and 120 h, and after 24 h exposure to insecticide plus 96 h on untreated leaf discs. Differences in the susceptibility of pests and parasitoids to different insecticides were marked for P. xylostella and C. vestalis, LC₅₀ values being significantly lower for the pest species. Such differences were not observed for M. persicae and A. colemani. There was a direct relationship between dose, exposure time and toxicity for all insecticides tested. All insecticides tested showed lower toxicity to both parasitoids compared with P. xylostella, which suggests that for this pest species side-effects on parasitoids can be minimised through IPM practices that reduce exposure time to such non-target organisms.     

Response of two tephritid species, Bactrocera oleae and Ceratitis capitata, to different emission levels of pheromone and parapheromone

Attractants and pheromones are commonly used in integrated pest management programs in crop systems. However, pheromone dispensers employed in monitoring traps and lure and kill devices are not usually well studied and attractants are released at uncontrolled rates leading to low treatment efficacies and misleading monitoring estimations. Fruit flies are pests of economic importance and monitoring is essential in order to program insecticidal treatments. Moreover, lure and kill techniques are being increasingly used, but the cost of these techniques depends on the number of required traps and, therefore, on the efficacy of the attractants. Ceratitis capitata and Bactrocera oleae are the two main fruit flies in Mediterranean countries, and the effect of different doses of trimedlure and spiroacetal on fly attraction has been studied. Results showed that a release rate over 1.28 mg/day of spiroacetal reduces B. oleae attraction and emission values over 2.4 mg of trimedlure per day did not increase C. capitata catches. Under the environmental conditions of our study, an optimum release rate for pheromone attraction in B. oleae was determined. Emission values over this optimum level reduced B. oleae attraction. However, when a parapheromone was used with C. capitata, a fruit fly of the same family, the optimum emission value was not found and higher quantities of parapheromone attracted the same number of flies. The saturation effect of high concentrations of pheromone and parapheromone is discussed.     

Survival of cabbage looper, Trichoplusia ni (Lepidoptera: Noctuidae), through winter cleanups of commercial vegetable greenhouses: Implications for insecticide resistance management

Cabbage looper, Trichoplusia ni (Hübner) (Lepidoptera: Noctuidae), is a highly migratory and destructive pest of various crops in North America. Its infestations in vegetable greenhouses in western Canada are generally attributed to annual immigrations from southern overwintering areas, but whether it survives overwinter in western Canada is uncertain. Bioinsecticides based on Bacillus thuringiensis (Bt) toxins are commonly used in southwestern British Columbia where some T. ni populations have developed strong resistance to Bt-insecticides. In this study, overwintering success of T. ni populations was monitored outside and inside three commercial greenhouses in British Columbia. In both years, T. ni pupae placed outside greenhouses did not survive although pupae placed inside the greenhouses survived the unheated cleanup period and exposure to organophosphate insecticides commonly used in the cleanup procedure in the greenhouses. During the cleanup period, no moths were caught inside the unheated greenhouses, but once the heating was restored for the new growing season, adults were captured in pheromone traps. No developmental stages of T. ni were detected on nursery seedlings and visual monitoring of transplants at the beginning of the season found no signs of previous feeding damage. This strongly suggests that T. ni were not introduced with the seedlings. Our findings are important for understanding the potential risks of inadequate winter cleanup and the potential carryover of resistance to Bt-insecticides in populations from one season to the next that could complicate pest management.     

Phenology and pest status of Agrotis segetum in a changing climate

Insects are strongly dependent on climatic factors, especially temperature. For this reason, changes in insect phenology are predicted to be one of the many effects of climate change. We analysed the effect of climate warming on Agrotis segetum (Denis & Schiffermüller) in Denmark. Agrotis segetum is an agricultural pest that causes root crop damage. Extensive registers of root crop damages by A. segetum in Denmark were kept between 1905 and 1980. These records show a correlation between A. segetum outbreaks and hot, dry summers. The last outbreak in Denmark, in 1976, prompted the implementation of pheromone trap monitoring. Data from these pheromone traps, for the periods between 1981–1991 and 1997–2009, show a decline in the number of A. segetum individuals during 2000–2009. We used this pheromone trapping data to test the hypothesis that temporal changes in phenology and abundance are correlated with temperature and precipitation patterns. As phenological fixed points, we use the time of capture (first, 10%, 50%, 90% and last) of 1st generation A. segetum together with the 10%–90% capture period and the total capture per location for a statistical comparison of the period 1981–1990 versus 2000–2009. We also analysed the effect of temperature on the timing of fixed points and the effect of precipitation on the mean total capture from the summer and the preceding winter. Our analyses showed an earlier occurrence of A. segetum during 2000–2009 than during 1981–1990: all phenological fixed points advanced by 6–9 days. Trap captures of the 1st generation roughly halved from the 1981–1990 decade to the 2000–2009 decade, and a much larger 2nd generation of moths developed in the latest decade. When a second generation occurs, populations in following years decrease because the larval offspring of the 2nd generation cannot successfully overwinter. Our analyses further indicated that increased precipitation during the summers and mild winters contributed to an overall decline in the populations of this pest. The presence of A. segetum remains sufficient to be a potential threat to yields. Forecasting an economically important attack of A. segetum is challenging because of the early emergence of A. segetum and more variable local weather conditions than 20 years ago; forecasting relies on the timely and correct delivery of monitoring results. Farmers are a vital source of information for monitoring, for estimating control requests and for timing the emergence of A. segtum. However, the growing frequency of no-risk forecasts has reduced the interest and lowered the motivation of farmers to participate in monitoring programs.     

Sublethal effect of avermectin and acetamiprid on the mortality of different life stages of Brontispa longissima (Gestro) (Coleoptera: Hispidae) and its larvae parasitoid Asecodes hispinarum Bouček (Hymenoptera: Eulophidae)

The beneficial parasitoid Asecodes hispinarum Bouček plays an important role in integrated pest management (IPM) of the coconut leaf beetle, Brontispa longissima (Gestro), in China. A. hispinarum females parasitize 3rd to 4th instars B. longissima larvae. Hatched parasitoid larvae develop within the host, and parasitoid adults emerge through holes that they chew through the cuticle of the host. Although chemicals serve as the main short term control agents, the compatibility of biological and chemical control has never been investigated for this system. This study examined the responses of immature and adult B. longissima and its larval parasitoid A. hispinarum to avermectin and acetamiprid. Avermectin caused complete mortality of 2nd to 4th instar larvae, and of adults of B. longissima at 10, 15 and 2 d after treatment, respectively. However, 26.7% of the 2nd instar larvae, 55.3% of the 4th instar larvae, and 74%, of adult B. longissima were still alive 40 d after acetamiprid application. Following avermectin exposure, 17.5%, 9.2% and 23% of mummified B. longissima larvae contained viable adult parasitoids for the parasitoid egg, larva and pupa treatments, respectively, and the numbers of dead parasitoids per mummy were 3.3, 7.2 and 13.3 for the egg, larva and adult treatments, respectively. However, for acetamiprid treatment, 70–75.9% of mummified B. longissima larvae contained viable adult parasitoids in all three stage treatments, and the number of dead parasitoids per mummy was 2.8, 2 and 3.4 in egg, larva and adult treatments, respectively. This study showed that a sublethal dose of avermectin is more toxic than acetamiprid to B. longissima and A. hispinarum. Therefore, direct contact of the parasitoid with avermectin should be avoided when this insecticide is used to control B. longissima.     

The effect of ridge planting and earliness of durum wheat varieties on sunn pest (Eurygaster spp.) damage and grain yield

Sunn pests (Eurygaster spp.) are among the most important insect pests of wheat (Triticum sativum L.) and barley (Hordeum vulgare L.). Cultural practices can prevent or minimize common sunn pest (Eurygaster integriceps Puton) population densities and crop damage. A three-year field experiment (Jun 05 to Aug 07) was conducted in Diyarbakır in the southeast Antolia region of Turkey, to evaluate the effects of planting method (no-till ridge planting and conventional planting) and earliness of durum wheat (Triticum durum L.) varieties (early, mid-season and late varieties) on E. integriceps density, grain damage and overall grain yield. The effect of the planting method on the sunn pest density and percent of grains damaged by sunn pest was not significant; however, there was a significant difference among the different varieties used in this study. While Çeşit-1252 (late wheat variety) had the highest nymph density (11.2 nymphs m⁻²) and percent of sunn pest damaged grains (2.8%), Sarıçanak-98 (early wheat variety) had the lowest nymph density (4.3 nymphs m⁻²) and percent of sunn pest damaged grains (0.8%). Grain yield was the highest (5.99 t ha⁻¹) for Sarıçanak-98 while there was no significant difference between Aydın-93 (5.06 t ha⁻¹) and Çeşit-1252 (4.99 t ha⁻¹). In conclusion, this study suggests that planting methods may not have a significant effect on sunn pest density, percent of sunn pest damaged grains or grain yield; however, the use of early durum wheat varieties, such asSarıçanak-98, may reduce sunn pest population densities, percent of damaged grains, allowing increases in grain yield in southeast Anatolia region, Turkey.     

Aphidophagous Parasitoids can Forage Wheat Crops Before Aphid Infestation,Parana State,Brazil

Aphid parasitoids are common in Brazilian wheat fields, and parasitize aphids at the wheat tillering stage. However, there is little information available about when this natural enemy occurs in wheat crops. This study investigated the initial occurrence of aphid parasitoids in four commercial wheat crops in northern Paraná during the 2009 crop season. We installed two Malaise traps at each wheat farm, and 400 tillers were assessed weekly in each field for aphid abundance. During this study, we captured 4,355 aphid parasitoids and 197 aphids. Three species of braconid parasitoids were identified, including Aphidius colemani (Viereck 1912), Lysiphlebus testaceipes (Cresson 1880), and Diaeretiella rapae (McIntosh 1855). The aphids species identified were Rhopalosiphum padi (Linnaeus 1758) and Sitobion avenae (Fabricius 1775). This study showed that aphid parasitoids are present in wheat crops even when aphid densities are low, and in one farm, occurred before the aphids colonization. These reports can justified the high efficiency of these natural enemies against aphids in wheat fields.     

Combining 1,4-dimethoxybenzene,the major flower volatile of wild strawberry Fragaria vesca,with the aggregation pheromone of the strawberry blossom weevil Anthonomus rubi improves attraction

The aggregation pheromone of strawberry blossom weevil [Anthonomus rubi Herbst (Col.: Curculionidae)], a 1:4:1 blend of Grandlure I, II and racemic lavadulol, has been available for pest monitoring for several years but shows low attractancy. Attempts to control A. rubi using the pheromone alone were also unsuccessful. This paper reports the finding that addition of the major flower volatile from wild strawberry flowers [Fragaria vesca L. (Rosaceae)], 1,4-dimethoxybenzene (comprising 98% of the volatiles emitted from wild strawberry flowers), to the aggregation pheromone increased trap catches by over two fold compared to the pheromone alone. There was no significant difference between the response of overwintered or summer emerged adults. Field trials in 2007–2008 in central and southern Norway, Denmark and southern England used green funnel traps with white cross vanes for the evaluations. (-)-Germacrene D, previously shown to be emitted by plants in increased amounts in the presence of pheromone producing weevils, did not improve trap catches. Thus, the combined use of the aggregation pheromone and 1,4-dimethoxybenzene is promising for improved monitoring and possibly control of this important pest of strawberry.     

Sublethal effects of spinosad and emamectin benzoate on larval development and reproductive activities of the cabbage moth,Mamestra brassicae L. (Lepidoptera: Noctuidae)

Lepidopteran insect pest management has relied heavily on synthetic chemical pesticides, but their efficiency is declining as a result of emerging insecticide resistance. Recently biopesticides have become the most promising products employed in pest management strategies. We investigated the sublethal effects of two bioinsecticides, spinosad and emamectin benzoate, on larval and pupal development, and reproductive activity including calling behaviour, pheromone production, fecundity and fertility of the cabbage moth, Mamestra brassicae. To assess sublethal effects, second instar larvae were fed with 0.005, 0.05, or 0.5 μg a.i. spinosad/g diet or 0.00005, 0.0005, or 0.005 μg a.i. emamectin benzoate/g diet. Both bioinsecticides significantly increased larval and pupal development time and negatively affected reproductive activity of M. brassicae. The calling activity of females decreased very significantly in the highest sublethal concentration of spinosad and in all treatments by emamectin benzoate. The results suggest that, both spinosad and emamectin benzoate are promising alternatives to conventional insecticides for the control of M. brassicae if successfully introduced into Integrated Pest Management (IPM) programs.     

Assessment of the effects of crop injury by blackcurrant leaf midge,Dasineura tetensi (Rübsaamen) (Cecidomyiidae) on yield and growth in commercial blackcurrant plantations

Sex pheromone trap catches, galling damage, yield and growth were recorded over up to three successive seasons (2010–2012) in large replicated plots treated versus untreated with synthetic pyrethroid (SP) insecticides for blackcurrant leaf midge Dasineura tetensi control in eight established commercial blackcurrant plantations in southern England. The aim was to determine whether or not the current practice of spraying insecticides against the pest in commercial fruiting plantations is justified. In two separate small plot replicated experiments (in 2010 and 2011, respectively) in plantations which had been cut down in the previous winter, galling damage and shoot growth were recorded in the vigorous regrowth in plots treated versus untreated with SP insecticides against 1st, 2nd or both D. tetensi generations. Cutting down bushes to ground level in this way is a common way of regenerating plantations.In the experiments in the established fruiting plantations, applying sprays of insecticides led to substantial reductions in numbers of adult D. tetensi caught in sex pheromone traps (by 72% on average) and reductions in galling damage (by 75% on average) but did not result in increases in yield or statistically significant increases in shoot growth. Multiple possible mechanisms of yield compensation are discussed. In the experiments in the cut-down bushes where the galling was intense, the D. tetensi damage resulted in severe (59%) stunting and multiple branching of the regrowth which was weak and thin. Sprays against the 2nd generation only partially reduced stunting (to 24%).The results of this work call into question the current commercial practice of controlling this pest in established commercial plantations with sprays of insecticides: as significant increases in yield or growth did not result in these experiments, the effects of the sprays was largely cosmetic. Further, broad spectrum synthetic pyrethroid insecticides are likely to have persistent adverse effects on the natural enemies of D. tetensi. However, the work showed that control in re-growing cut-down plantations, and by analogy in young establishing plantations, is vital, as well as the value of the D. tetensi sex pheromone traps for timing sprays of insecticides.     

Disruption of Darna pallivitta (Lepidoptera: Limacodidae) by Conventional and Mobile Pheromone Deployment

Identification of the Darna pallivitta (Moore) pheromone component n-butyl (E)-7,9-decadienoate (E7,9-10:COOn-Bu) has made it possible to investigate communication disruption to control this lepidopteran pest. Conventional communication disruption trials showed marked decreases in the mean number of male moths captured in E7,9-10:COOnBu-treated fields compared with control fields. For traps baited with E7,9-10:COOnBu, percent disruptions were 94.4% and 92.1% for septa (1 g pheromone/ha, 1-wk trial duration) and spirals (6 g pheromone/ha, 8-wk trial duration) respectively. For traps baited with virgin female moths, percent disruption was 73.3% using septa disruptors (1 g pheromone/ha, 1-wk trial duration). Mobile communication disruption using Bactrocera cucurbitae (Coquillett) as carriers for E7,9-10:COOn-Bu was evaluated in the following three areas: fly survivorship, attraction of male moths to treated flies, and moth disruption in a small-scale field trial. Topical application of E7,9-10:COOnBu showed no significant decrease in survivorship at 50 and 80 µg/fly. However, decreased survivorship was observed at 100 µg/fly and linear regression showed E7,9-10:COOnBu dose was significantly correlated with B. cucurbitae survivorship. Traps containing honey–pheromone-fed flies attracted and caught D. pallivitta over a 1-wk period, demonstrating the attractiveness of the carrier. Releasing E7,9-10:COOnBu-fed B. cucurbitae (∼2 g pheromone/ha, 1-wk trial duration) resulted in significantly reduced trap catches in treatment fields compared with control fields on the first 2 d of the field trial. Percent disruptions were 84.7% (day 1) and 56.0% (day 2). These results suggest that both conventional communication disruption and mobile communication disruption have potential to control D. pallivitta.     

A straw mulch system to allow continuous wheat production in an arid climate

Cereal Cyst Nematode (CCN, Heterodera avenae Woll.) has been shown to be a devastating pest for wheat (Triticum aestivum L.) in dryland regions. Fallowing in the season preceding the cropping season has been hypothesized to sanitize the soil of CCN and allow wheat production. This paper explores management options that might allow the continuous production of wheat in these regions. In a 20-year study in the Negev, Israel, on a sandy loam, loessial, soil, it was found that in those seasons with high rainfall there was virtually no decrease in annual wheat yields for continuous crops as compared to biennial fallow yields obtained with the conventional wheat system. The hypothesis that high soil water content substantially alleviates the damage resulting from CCN infestation was confirmed in a pot study. A practical solution for maintaining high soil water content in the field was to leave a straw mulch on the soil surface to decrease soil evaporation. A chopper was added to a grain harvester to finely chop the straw so that it settles to the soil surface through the stubble, and a no-till drill was used for sowing through the straw. The straw-mulch system was shown to result in annual yields from continuous wheat that were equivalent to yields in alternate years with the conventional fallow wheat system, thereby doubling wheat production in this dryland region.     

Interactions between Metopolophium festucae cerealium (Hemiptera: Aphididae) and Barley yellow dwarf virus (BYDV-PAV)

Interactions between an invasive aphid, Metopolophium festucae (Theobald) subsp. cerealium, and Barley yellow dwarf virus (BYDV-PAV) were studied under laboratory conditions. M. festucae cerealium is an economic pest of wheat and barley that has recently been found in high population densities in wheat in the Pacific Northwest of the United States. BYDV-PAV is the most prevalent and injurious species of BYDV worldwide and in the Pacific Northwest. Although M. festucae sensu stricto (Theobald 1917) has been reported previously as a vector of some BYDV isolates, there is no confirmed transmission of BYDV by M. festucae cerealium. Two experiments examined the ability of M. festucae cerealium to transmit BYDV-PAV. The first used single aphids caged to indicator plants of a BYDV-susceptible winter wheat cultivar and the second used multiple aphids on each test plant. M. festucae cerealium did not transmit BYDV-PAV in either experiment, whereas transmission by a known BYDV vector, Rhopalosiphum padi L., was consistently high (≥93%). A third experiment compared the intrinsic growth rate, days until first reproduction and daily reproduction by M. festucae cerealium on sham-inoculated and BYDV-PAV-infected wheat, but detected no differences. The findings are reviewed in light published data on M. festucae species, BYDV transmission, and the potential pest status of this new invading aphid.     

Pest behaviour insights from quarantine surveillance of male Queensland fruit fly,Bactrocera tryoni (Froggatt) (Diptera: Tephritidae)

Fruit flies (Diptera: Tephritidae) are a major problem for Australia's $6.9 billion horticultural industry. Bactrocera tryoni (Froggatt) is the most significant fruit fly pest in the eastern states of Australia. Catches of male B. tryoni from cuelure-baited Lynfield surveillance traps in towns bordering the Fruit Fly Exclusion Zone in New South Wales, Australia, from November to May in 2001/2002–2007/2008 were analysed with respect to year, town, property type (urban/rural), and tree species/crop varieties in which the trap was placed (tree type). Average catches of all traps were highest in 2007/2008 and lowest in 2003/2004, and showed an overall positive correlation with rainfall (P < 0.05). More B. tryoni males were caught in Wagga Wagga and Lockhart than in other towns. There were no significant differences in catches of B. tryoni between urban and rurally located traps. With respect to tree type, traps placed in pomefruit trees caught significantly more flies than those in citrus, stonefruit, or nonfruit trees, with no significant differences among the latter three tree groups. Within the pomefruit group, traps in apples (Malus domestica Borkh.) and pears (Pyrus spp.) caught significantly more flies than those in cotoneasters (Cotoneaster spp). Within the citrus group, traps in orange trees (Citrus × sinensis (L.) Osbeck) caught significantly more flies than those in mandarin trees (Citrus reticulata Blanco). No significant differences were detected in catches between any members of the stonefruit group. Within the nonfruit group, traps placed in kurrajongs (Brachychiton populneum (Schott & Endl.) R. Br.) caught significantly more flies than those placed on other tree species in the group. This study demonstrates that although surveillance programs conducted to establish and/or maintain area freedom from specific pests may not provide balanced datasets, careful analysis of the results of such programs can provide valuable insights into the behaviour of the species in question.     

Assessing the impact of areawide pheromone trapping,pesticide applications,and eradication of infested date palms for Rhynchophorus ferrugineus (Coleoptera: Curculionidae) management in Al Ghowaybah,Saudi Arabia

The red palm weevil, Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae) is a highly destructive pest of date palms, Phoenix dactylifera L. (Arecales: Arecaceae), in Saudi Arabia. Data spanning a six year period (2007–2012) from Al Ghowaybah, a 1104 ha date producing region in the Al Ahsaa Directorate in Saudi Arabia, were analyzed to assess the impact enhanced management efforts that commenced in Oct. 2009 had against this pest. Within six months of initiating the areawide management program significant reductions in the mean monthly number of weevils trapped and percentage traps with R. ferrugineus were detected. Mean monthly trap captures of R. ferrugineus and the percentage of traps capturing weevils declined significantly from 2009 to 2012 by an average of 65% and 90%, respectively, indicating that trapping and dispersal pressure was significantly reduced. By 2011, average monthly trap captures and percentage of traps with R. ferrugineus were significantly lower than all pre-management capture data and this was maintained through 2012 when data collection ceased. Additionally, over the period 2010–2012, insecticide application and palm eradication rates dropped by 91% and 89%, respectively. The total number of R. ferrugineus captured in 2012 declined by 86% when compared to total captures for 2010. At the end of 2012, the estimated infestation rate of date palms in Al Ghowaybah was 0.36%, which was below the economic threshold of a 1% infestation rate set by the Directorate of Agriculture supervising the program. It is concluded that the mandatory areawide management program that commenced in Oct. 2009 against R. ferrugineus in Al Ghowaybah had a significant and rapid impact against this pest.     

Development of site-specific IPM against European grapevine moth Lobesia botrana (D. & S.) in vineyards

The aim of this study was to evaluate the potential for site-specific IPM of the European grapevine moth, Lobesia botrana (Denis & Shiffermüller), in a Mediterranean agricultural landscape in central Italy. In 2005-2006, sampling was conducted to verify the spatio-temporal distribution of L. botrana eggs, larvae and adult populations in the investigated agro-ecosystem. On the basis of these results, we applied tactics in 2007 and 2008 to reduce the outer source of the infestation by establishing a pheromone trap barrier system to prevent the movement of male adults from the olive groves into the vineyards, and to test the hypothesis that a within-field site-specific control results in reduced insecticide requirements compared with a traditional uniform IPM protocol. Sampling and control operations were carried out to avoid an increase of economic and labour efforts, leaving as much as possible the costs at the same level of a standard monitoring program. The results highlighted that male hot spots in olive groves disappeared, and that the number of larval nests on vine inflorescences was significantly decreased, when additional traps were deployed (2007-2008) compared to the period before (2005-2006). The site-specific control, i.e. treating only egg hot spots with Bacillus thuringensis var. kurstaki, allowed for a decrease in the surface of the treated vineyard and, consequently, the quantity of insecticide utilised. In 2008, no significant differences between uniform and site-specific IPM in vineyards were observed in the number of damaged berries and the percentage of infested bunches. The results are discussed with respect to the spatial pattern of pest populations, site-specific IPM tactics, sampling operations and pest control costs.