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1.
Foliar sprays of pesticides are frequently used to control two-spotted mites on rose flowers, but these also destroy predatory mites and pose a high risk of contamination for humans and the environment. Using a novel approach to avoid spray applications on rose plants, modified acaricide-treated string curtains were adapted to control the pest. Two main aims of this study were: (i) to identify the lethal concentration of string curtains treated with propargite, dicofol, flufenoxuron, acrinathrin or tau-fluvalinate (τ-fluvalinate) on Tetranychus urticae, and (ii) to test the design (feasibility) and efficiency of the string curtains to trap the phytophagous mites on the plants during their circadian migration. Bioassay results in the lab confirmed T. urticae circadian migration, toxicity of the five kinds of treated string curtains and a concentration-dependent repellent effect of each chemical on T. urticae females. Of the two products tested in the field, dicofol was more effective than acrinathrin in controlling T. urticae compared to an acaricidal spray of the same products. The effect of acaricide-treated string curtain use on Phytoseiulus persimilis did not differ significantly from the control. The string curtain technique proved to be effective, but because of some biotic and abiotic constraints and the low economic threshold of mites, even for the commercialized high canopy stem roses, the present device could be more appropriate for another horticultural crop, e.g. tomato. 相似文献
2.
Experimental releases of the predatory mite Kampimodromus aberrans (Oudemans) were conducted in organic and conventional apple (Malus domestica Borkh.) orchards located in North-eastern Italy. Releases were made in 2010 and 2011 and observations were carried out from 2010 to 2012. The material used for releases was collected from a commercial vineyard where K. aberrans was the dominant phytoseiid species. The most frequent insecticides used in organic orchards were pyrethrins and spinosad, whereas neonicotinoids, organophosphates and insect growth regulators were mostly used in conventional orchards. K. aberrans population levels were significantly higher in release plots as compared to control plots and K. aberrans populations were higher in organic compared to conventional orchards. In 2010, no effects of K. aberrans release and orchard management were observed on populations of the native predatory mite Amblyseius andersoni (Chant). However, in 2011 A. andersoni population densities were lower in release than in control plots. In conventional orchards, K. aberrans released in 2010 and 2011 did not establish probably because of a series of non-selective insecticide and fungicide treatments. In one experimental site, releases were evaluated in two organic orchards, one of Florina cv. and the other of Golden Delicious cv., managed with the same cropping systems. On Florina, K. aberrans population appeared to be larger than on Golden Delicious suggesting a potential role of leaf morphology in predatory mite colonization. On Golden Delicious, A. andersoni population densities were lower in release than in control plots. Implications for mite management in organic and conventional orchards are discussed. 相似文献
3.
Among 19 bacterial strains isolated in Yunnan from rhizosphere soils and plant tissues, Bacillus methylotrophicus strain R2-2 and Lysobacter antibioticus strain 13-6 exhibited the highest antagonistic activity against the tomato root-knot nematode Meloidogyne incognita in plate and greenhouse pot experiments. The two strains, when applied as soil drenches or seed treatments in greenhouse experiments, reduced root-knot severity and incidence on tomato compared to no-bacteria controls. In tomato field trials conducted in separate years, soil drench treatment with either strain reduced root-knot disease levels and increased yields compared to the control. Levels of disease control and yield enhancement provided by the strains were higher than those using the chemicals abamectin and carbofuran. This is the first report of B. methylotrophicus being used as a biocontrol agent against a plant parasitic nematode and the first demonstration that B. methylotrophicus and L. antibioticus can suppress disease caused by root-knot nematodes in the field. 相似文献
4.
The aim of this study was to evaluate the effectiveness of the entomopathogenic fungi (EPF), Beauveria bassiana (Bals.) Vuill. (Deuteromycotina: Hyphomycetes) strain PPRI 5339 [BroadBand, an emulsifiable spore concentrate (EC) formulation] and Metarhizium anisopliae (Metsch.) Sorokin (Hypocreales: Clavicipitaceae) strain F52 [Met52, both EC and granular (GR) formulations] against the larvae of Polyphylla fullo (L.) (Coleoptera: Scarabaeidae). Larvicidal bioassays were performed in foam boxes (100 by 75 by 50 cm; length by width by height), containing moist soil medium with some humus and potato tubers as food. Although the B. bassiana product (min. 4 × 109 conidia/ml) was applied at 100, 150, and 200 ml/100 l water; M. anisopliae strain F52 was applied at 500, 1,000, and 1,500 g/m3 of moist soil medium for GR (9 × 108 cfu/g) and 75, 100, and 125 ml/100 l water for EC (5.5 × 109 conidia/ml) formulation. Both fungi were pathogenic to larvae of the pest; however, young larvae (1st and 2nd instars) were more susceptible to infection than older ones (3rd instar). Mortality rates of young and older larvae varied with conidial concentration of both fungi and elapsed time after application. The B. bassiana product was more effective than both of the formulations of the M. anisopliae product, causing mortalities up to 79.8 and 71.6% in young and older larvae, respectively. The highest mortality rates of young and older larvae caused by the M. anisopliae product were 74.1 and 67.6% for the GR formulation, 70.2 and 61.8% for the EC formulation, respectively. These results may suggest that both fungi have potential to be used for management of P. fullo. 相似文献
5.
Wireworms are the soil inhabiting larvae of click beetles and can cause severe damage to arable crops such as potatoes (Solanum tuberosum, L.). Several strains of the entomopathogenic fungus Metarhizium brunneum (Petch) are pathogenic to wireworms. In this study, three European strains of M. brunneum were tested in the laboratory against the most damaging wireworm species in Europe, Agriotes lineatus (L.), Agriotes obscurus (L.) and Agriotes sputator (L.). A Swiss strain, isolated from an A. obscurus cadaver, proved to be most effective, killing up to 73% of A. lineatus and 83% A. obscurus individuals, respectively. The median lethal time (LT50) was 21 days post inoculation (dpi) for A. lineatus and 14 dpi for A. obscurus. The strain did not lose virulence through subsequent cultivation on artificial medium and thus seems to be suitable for mass production as a biocontrol agent for wireworm control. 相似文献
6.
The state of the art for controlling four primary pests or pest complexes by augmentative releases of predators and parasitoids in the United States is reviewed. The pests are (1) the bollworm, Helicoverpa zea (Boddie) and tobacco budworm, Heliothis virescens (F.), (2)_the boll weevil, Anthonomus grandis grandis (Boheman), (3) the pink bollworm, Pectinophora gossypiella (Saunders) and (4) plant bugs, specifically Lygus spp. Rearing of the predators and parasitoids and identification and the use of behaviour-modifying chemicals are described and discussed. 相似文献
7.
Three isolates, AS3 (non-aflotoxigenic Aspergillus flavus Link), TRIC7 and TRIC8 (Trichoderma harzianum Rifai), from onion (Allium cepa L.) growing soils were recently found to control black mold disease caused by Aspergillus niger (An) van Tieghem and to increase the accumulation of antifungal compounds in pot-grown onion sets. Their ability to increase bulb diameter and total soluble solids in marketable bulbs, to control black mold and to induce the production of antifungal compounds were tested in sets and marketable bulbs raised from treated seeds and sets, respectively, in naturally An-infested field soils at two locations. These isolates significantly controlled the disease at both locations, but they did not have any enhancing effect on set or bulb diameter and soluble solids in marketable bulbs. AS3 and TRIC8 in particular led to defense reactions with accumulation of antifungal compounds in sets and marketable bulbs in both locations. Different compounds were also identified in the fractions with highly antifungal effects. 相似文献
8.
The tomato red spider mite, Tetranychus evansi Baker and Pritchard, is one of the most serious pests of solanaceous crops in Africa. Field experiments were conducted to investigate its effects on the growth and leaf yield of five African nightshade species viz. Solanum americanum, S. sarrachoides, S. scabrum, S. tarderemotum and S. villosum during the 2008 and 2009 growing seasons. Plants were infested with 2–4 day-old female spider mites which were allowed to multiply. The number of mite motiles increased throughout the growing season in unsprayed plots and this number varied significantly between the African nightshade species. Except for S. sarrachoides, leaf damage was high on the other four Solanum species irrespective of the spraying regime during both seasons. However, S. scabrum had a significantly greater leaf area ratio (ratio of leaf area to total plant weight) and specific leaf area (ratio of leaf area to total leaf dry weight) during both seasons. Overall yields were 1.5 times more in S. scabrum and S. sarrachoides compared to S. americanum, S. tarderemotum and S. villosum. Our results show that T. evansi infestation affects the leaf area ratio and specific leaf area of African nightshade species differentially which eventually determines the plant's overall leaf yield. These findings present an opportunity for evaluation and selection of African nightshade species that can withstand spider mite infestation in small holder farms for increased vegetable production in Africa. 相似文献
9.
The fungus Pochonia chlamydosporia acts on populations of the root-knot nematode by parasitizing their eggs before they hatch, preventing the release of juveniles. In this study, the effect of the time at which the fungus is applied to soil infested with the nematode, before transplanting tomato and lettuce seedlings, was evaluated. The reduction of symptoms and the nematode population in the host plant was also evaluated, as well as the fluctuation of the fungus population during the studied period. The seedlings were transplanted at 0, 5, 10, 15 or 20 days after incorporation of the inocula of the fungus and the nematode into the soil. The population of P. chlamydosporia, without the presence of plants, increased and reached its maximum 10 days after its incorporation into the soil. At the end of the experiments, the number of colony forming units (CFU) of the fungus in the soil remained high enough to act on the next cycle of crops. For both cultures, the greatest effect on reducing the number of galls and eggs of Meloidogyne javanica was obtained by increasing the contact time of the fungus with the nematode in the soil before transplanting seedlings. In the second run, for both cultures, there was an increase in shoot weight with increasing of incubation time before the transplantation of seedlings. 相似文献
10.
Four experimental trials were carried out in northern Italy under greenhouse conditions against bacterial leaf spot of tomato, incited by Pseudomonas syringae pv. syringae, a disease recently observed in the country. The efficacy of spray programmes, based on the use of several copper-based compounds, acibenzolar-S-methyl, fosetyl Al and two biological control agents, Bacillus subtilis strain QST 713 and Pseudomonas chlororaphis MA 342, was tested. The best results were obtained with the alternation of copper oxychloride, used alone or in mixture with acibenzolar-S-methyl, with acibenzolar-S-methyl, applied for a total of two sprays. Acibenzolar-S-methyl applied alone was also effective. However, due to its possible slight phytotoxic effect, observed in our case in one trial, strategies relying on a limited number of its application are preferred. The efficacy of acibenzolar-S-methyl is higher under lower inoculum conditions. Among the tested copper-based fungicides, copper oxychloride provided the best and most consistent results, with no or limited phytotoxicity. Other copper compounds were much less effective and sometimes caused a reduction in plant height. The disease suppression achieved with B. subtilis strain QST 713 was only partial, while P. chlororaphis was not effective. The advantages of programmes based on rotation of copper compounds and acibenzolar-S-methyl are discussed. 相似文献
11.
Impact of preharvest application of biological control agents on postharvest diseases of fresh fruits and vegetables 总被引:9,自引:0,他引:9
During the storage of harvested commodities, environmental parameters are quite stable. For this and other reasons, it is generally believed that biological control by means of microbial antagonists may have a greater potential for success when applied postharvest. However, one of the major obstacles to the development of postharvest biocontrol agents is that they are unable to control previously established infections, such as latent and quiescent infections and incipient infections occurring through wounds resulting from harvesting operations. Field application of biocontrol agents may enable early colonisation of fruit surfaces, thus protecting from these infections. Moreover, preharvest applications can be an appropriate strategy for fruits and vegetables subject to damage in postharvest handling. To be successful in preharvest applications, putative biocontrol agents must be able to tolerate low-nutrient availability, UV radiation, high temperature and dry conditions. Some reports of postharvest biological control accomplished by preharvest applications, include apples, avocados, sweet cherries, grapes, and strawberries. This paper provides a brief overview on particular aspects of preharvest application of biocontrol agents to reduce postharvest decay. Research areas relevant for the development of this strategy are also indicated. 相似文献
12.
13.
Nguya K. Maniania 《Crop Protection》1993,12(8):601-604
The fungus Beauveria bassiana (Bals.) Vuill. and insecticide trichlorfon were compared for the control of stem borer, Chilo partellus (Swinhoe) in field experiments at different times of infestation. Egg masses of C. partellus at the blackhead stage were pinned to the undersurface of the maize leaves. Two fungal formulations, conidial aqueous suspensions and a granular formulation, were applied. Compared with the untreated checks, the numbers of surviving C. partellus larvae were significantly reduced in treatments where B. bassiana was applied at a concentration of 1013 conidia ha−1 as granules and as two aqueous spray formulations. No significant difference in numbers of stem borer larvae was found between trichlorfon and the untreated check. Fungus granules persisted longer in the field than did one spray of the fungus inoculum at the same concentration, or trichlorfon. A major increase in grain yield was obtained with two fungal sprays and with fungus granules both applied at a concentration of 1013 conidia ha−1. A granular formulation of B. bassiana should be considered for the control of the stem borer C. partellus. 相似文献
14.
Markis Adames Manuel Fernández-Ruvalcaba Guadalupe Pe?a-Chora Victor M. Hernández-Velázquez 《Journal of insect science (Online)》2011,11(1)
The aim of this work was to assess the virulence of strain M379 of the fungus,
Metarhizium anisopliae (Metchnikoff) Sorokin (Hypocreales:
Clavicipitaceae) after different passages through a suitable host and at different
concentrations for the control of both acaricide-susceptible and resistant strains of
the tick, Rhipicephalus (formerly Boophilus)
microplus Canestrini (Ixodida: Ixodidae) in vitro. The
highest value of LC50 for the susceptible strain corresponded to zero
passage with 7.68 × 107 conidia/ml followed by the fourth passage
with 2.68 × 107, which reduced 2.87-fold the lethal concentration.
When comparing LC50 values of the fourth vs. the seventh passage (2.59 ×
105 conidia/ml), the lethal concentration was reduced 103.47-fold by
the seventh passage. In addition, in the resistant strain the LC50 highest
value corresponded to zero passage with 4.95 × 107 conidia/ml
followed by the fourth passage with 7.86 × 106, which reduced
6.30-fold the lethal concentration. When comparing LC50 values of the
fourth vs. the seventh passage (1.04 × 105 conidia/ml) in the
resistant strain, the lethal concentration was reduced 75.58-fold by the seventh
passage. These results suggest that the number of passages on M.
anisopliae through a suitable host increased its virulence on both
R microplus strains. When comparing LC50 of the zero
passage through a suitable host of both acaricide-susceptible and resistant strains,
the highest LC50 values corresponded to the susceptible strain with 7.68
× 107 conidia/ml followed by the resistant one with 4.95 ×
107, showing that on the resistant strain the lethal concentration is
reduced by 1.55-fold. When comparing the fourth passage, the highest values of
LC50 corresponded to the susceptible strain with 2.68 ×
107 conidia/ml followed by the resistant one with 7.86 ×
106 conidia/ml, showing for the resistant strain a 3.41-fold reduced
lethal concentration. Moreover, when comparing the seventh passages, the highest
values of LC50 corresponded to the susceptible strain with 2.59 ×
105 followed by the resistant with 1.04 × 105
conidia/ml, revealing for the resistant strain a 2.49-fold reduced lethal
concentration. These results suggest that the resistant strain needs a lower
concentration of conidia than the susceptible strain. In this case, the
acaricide-resistant strain is more susceptible to M. anisopliae of
zero- and seven-passage strains. 相似文献
15.
A.A. Boling T.P. Tuong H. Suganda Y. Konboon D. Harnpichitvitaya B.A.M. Bouman D.T. Franco 《Field Crops Research》2008
A large proportion of rainfed lowland rice in Southeast Asia is grown in gently sloping areas along toposequences with differences in elevation of a few meters. These small differences in elevation can lead to differentiation in soil properties and hydrological conditions, which in turn may affect crop performance and yield. It may be appropriate to replace blanket crop management recommendations in rainfed areas with toposequence-specific management recommendations. However, thorough statistical analyses of the relationships between toposequence position and field and crop conditions are lacking. In this paper, we statistically analysed the effect of toposequence position on soil properties, hydrological conditions, yield, and yield increase due to weed control and/or fertilizer management in rainfed areas in four villages in Indonesia and Thailand each in 2000–2002. 相似文献
16.
This study investigated the efficacy of Glomus mosseae and thyme and peppermint oils in controlling bacterial wilt disease (BW) of tomato caused by Ralstonia solanacearum under in vitro conditions and under greenhouse and field conditions. In an in vitro study, we tested nine plant oils against R. solanacearum, and the results demonstrate that only caraway, thyme, peppermint and marjoram oils inhibited the growth of the BW pathogen with differing degrees, thyme oil caused the highest reduction of the growth of the pathogen followed by peppermint and the lowest was caraway and marjoram. We evaluated the effect of thyme and peppermint under greenhouse and field conditions. Under greenhouse conditions, the thyme treatment exhibited the highest disease reduction percentage followed by the thyme + peppermint treatment, while G. mosseae caused the least disease reduction percentage. Under field experiments, thyme oil treatment exhibited the highest ability to reduce the disease by 94.8 and 97.1% in the 2011 and 2012 growing seasons, respectively, followed by the treatment of thyme oil + peppermint oil with the reduction of 89.5 and 82.4%, respectively. G. mosseae exhibited the lowest percentage of disease reduction; however, it caused a greater yield increase percentage during both of the tested seasons. 相似文献
17.
P.D. Roberts M.T. Momol L. Ritchie S.M. Olson J.B. Jones B. Balogh 《Crop Protection》2008,27(12):1519-1526
Bacterial spot caused by Xanthomonas euvesicatoria Jones et al. and Xanthomonas perforans Jones et al. is a major disease on fresh market commercial tomato in Florida. Fourteen field trials were conducted between 1999 and 2005 (10 in south Florida and four in north Florida) testing famoxadone plus cymoxanil (Tanos 50DF®, E.I. du Pont de Nemours and Company, Wilmington, DE), Bacillus subtilis strain QST 713 (B. subtilis) (Serenade WPO® or Serenade Max®, AgraQuest, Inc., Davis, CA), and acibenzolar-S-methyl (ASM) (Actigard 50WG®, Syngenta Crop Protection, Greensboro, NC) at different rates and in various application programs that were combined and rotated with copper hydroxide and mancozeb for management of bacterial spot. In field applied spray treatments containing famoxadone as a component, all of the programs significantly reduced bacterial spot severity on plants compared to the untreated control plants (UTC) and 97% of the programs were equal for disease suppression conferred by the copper-mancozeb standard. In spray programs containing ASM or B. subtilis plus copper hydroxide, treated plants had significantly reduced disease compared to the UTC plants and were not different from the plants treated with the copper-mancozeb standard. Yield data from small plots was unaffected. Several of the programs which used these compounds in rotation with copper-mancozeb provided similar levels of reduction in the disease severity for bacterial spot while reducing by 50% the amount of copper applied to plants. The effect of famoxadone plus cymoxanil on the survival of Xanthomonas in vitro did not cause a significant reduction in the bacterial population and was not determined to be directly bactericidal. However, greenhouse and field testing supports disease reduction of bacterial spot on plants treated with these compounds. The products tested in these trials may be useful, alternative tools for use in an integrated management program for bacterial spot on tomato. 相似文献
18.
A. Albu-Yaron A. Feigin I. Rylski 《Plant foods for human nutrition (Dordrecht, Netherlands)》1993,43(3):201-210
The quality of tomato fruit and juice (Lycopersicon esculentum L. cv. VF M82-1-8) grown in an aerohydroponic system in a greenhouse was affected by the level of Cl– and NO
3
–
, and by the osrnotic potential (OP) of the nutrient solutions. The total suspended solids (degrees Brix) in the fresh juice increased from approximately 4.0 in the nonsaline solutions (OP –0.05 MPa) to approximately 5.6–5.8 in the saline solutions (OP –0.45 MPa). Juice acidity was similarly affected by the Cl–, NO
3
–
and OP levels in the nutrient solutions. Less affected, the ascorbic acid content of juice, was lowest ( 8–9 mg/100 cc) in the high-NO
3
–
-nonsaline solution treatments, and was between 10 and 12 mg/100 cc at OP levels greater than –0.2 MPa. NO
3
–
in the juice was high ( 60 mg/L) under low OP conditions ( –0.05 to –0.1 MPa), especially when combined with high NO
3
–
levels, and lower (between 8 and 30 mg/L) in plants exposed to saline conditions (low OP). Fruit puffiness (boxiness) was reduced markedly by salinity and was not considerably affected by the NO
3
–
/Cl– ratio, while fruit firmness was influenced by both factors.Contribution from the Agricultural Research Organization (ARO), The Volcani Center, Bet Dagan, Israel. 相似文献
19.
花生、大豆是我国重要的油料和经济作物,因易受黄曲霉毒素污染,严重威胁食品安全,制约产业发展,因此,黄曲霉毒素防控一直是国内外研究的热点难题。本团队首次提出将黄曲霉毒素绿色阻控与促进根瘤固氮耦合的研究思路,研发了生物菌剂ARC-BBBE,本文报道生物菌剂ARC-BBBE对黄曲霉毒素及其产毒菌阻控的大田应用效果,调查其对花生生物学性状及经济性状的影响,探索ARC-BBBE对花生、大豆根系结瘤及根瘤固氮的影响,为从田间源头阻控黄曲霉毒素及促进根瘤固氮、实现减肥减药提供理论依据和科学指导。连续2年在我国花生主产省开展田间防控试验,将绿色阻控生物菌剂ARC-BBBE于花生播种期以30 kg/hm2用量与基肥一起撒施于土壤中。同时开展室内花生、大豆盆栽试验。使用涂布法调查土壤中黄曲霉毒素产毒菌丰度,使用高效液相色谱法检测花生样品中黄曲霉毒素,使用乙炔还原法测定根瘤固氮活性,采用5点取样法调查统计根瘤数、根瘤重。结果表明,应用ARC-BBBE显著降低了土壤中黄曲霉毒素产毒菌的丰度(平均降低66.5%)和花生中黄曲霉毒素的含量(平均降低83.5%)。应用ARC-BBBE后,花生根系普遍出现超级结瘤现象,实验验证表明其根瘤具有固氮活性,平均结瘤数增加10倍以上(土壤贫瘠地区50倍以上),瘤重增加8.8倍,每克根瘤固氮酶活性提高5倍以上。饱果率、产量均明显提高,叶色浓绿。花生室内盆栽结果表明,ARC-BBBE处理组根系结瘤数是对照组的2.2倍,固氮酶活性是对照组的4倍,叶绿素含量较对照组增加21.3%。大豆室内盆栽苗期实验结果表明,ARC-BBBE同样可诱发大豆结瘤及固氮效应:播种后第26天,处理组瘤数增加13.5倍、瘤重增加19.8倍;而且处理组有固氮活性,而对照组无固氮活性;苗期处理组的根长、根重、鲜重、叶绿素等生物学指标显著提升。ARC-BBBE对花生黄曲霉毒素及其产毒菌污染阻控效果极为明显,既可从生产源头有效阻控花生黄曲霉毒素产毒菌,降低黄曲霉毒素污染风险,同时大幅增加了花生根瘤数量和固氮酶活性,具有显著的促生长、增产量、控病害、保安全作用,具有显著的经济、社会、生态效益。对未来减施农药、化肥,保护农田生态,推动花生、大豆产业高质量绿色发展具有重要意义,在花生、大豆等豆科作物生产中应用前景广阔。 相似文献
20.
百草枯的控草效果及对光照和油菜产量的影响 总被引:4,自引:0,他引:4
为明确百草枯在免耕油菜田的应用前景,建立免耕油菜田的杂草管理体系,研究了百草枯对免耕油菜田杂草的控制作用及该药剂对田间光照和油菜产量的影响.结果表明,百草枯对免耕油菜田杂草具有较好的防除效果,药后第5d的总体防效在98.50%~99.49%之间,药效随时间的延长略有下降,160d内的控草效果仍可达85%以上.百草枯对田间主要杂草看麦娘(Alopecurus aequalis)、牛繁缕(Malachium aquaticum)及菵草(Beckmannia syzigachne)的防除效果较好,对通泉草(Mazusjaponicus)的效果较差.使用百草枯能显著提高油菜田间的透光率,随着用药量的增加,透光率逐渐上升.百草枯的施用对油菜株高、千粒重没有显著影响,但对油菜第一分枝高度、单株分枝数、角果数影响较大,能显著提高油菜产量. 相似文献