犬多头带绦虫感染情况调查

采用氢溴酸槟榔碱下泻法对天峻地区的犬进行了多头带绦虫感染情况调查。共检查94只犬，检出阳性犬6只，感染率为6．38％，检出虫体88条，平均感染强度14．67条。     

羊多头蚴的诊断与治疗

羊多头蚴病是由于多头带绦虫的幼虫即脑多头蚴寄生在羊的脑内,引起神经症状,甚至死亡的一种寄生虫病。1病原脑多头蚴,又称为脑包虫,属于绦虫类,是多头带绦虫的幼虫,呈现囊泡状,有的呈现豌豆粒大小,随着长到鸡蛋大小,囊内可以有一百多个原头蚴,一般有芝麻大小,原头蚴直径2-3mm。寄生在羊的脑内,是羊发生疾病的源头。     

久治地区牦牛多头蚴病的调查

多头蚴病俗称“脑包虫病”。是由多头带绦虫的幼虫——脑多头蚴所致的一种脑脊髓病。患畜主要是牛和羊,人也有被感染报道。久治地区牛多头蚴感染情况未见报道。为了掌握牛多头蚴病的感染情况,制定切实有效的防治措施。笔者在2001年3～5月对本县的400头幼年牛用随机抽样法进行调查。结果检出阳性牛13头,感染率3.25％,为本地区今后对牛多头蚴病的防治提供了依据。1 材料与方法1.1 本文所调查的牛均为久治县6个乡的400头2岁以内幼年牛。1.2 方法:从本县6个乡中随机抽样法抽10户,每户抽样2岁以内幼年牛40头,向畜主询问有无病史,用肉眼观察并用手触摸寄生部位,检出多头蚴病患畜。1.3 选4头症状典型的牛,按常规寄生虫学检查方法进行剖检。在患畜颅骨软化处,用外科解剖刀打开,仔细查找包囊,并计数登记,必要时注射器抽出囊内容物     

青海省部分地区牦牛、藏羊多头蚴感染情况调查

通过对青海省9个县牛羊多头蚴病发生情况的调查,发现不同地区多头蚴感染情况大不相同,发病地区主要以玉树州、果洛州、海西州、海北州等一些经济比较落后的地方较为严重,如囊谦的发病率为13.98%,久治为3.0%。通过对76头发病牦牛和50头藏羊进行了详细的年龄和病例出现时间分布统计,表明1~2岁牛羊感染率最高,成年牛羊感染率则很低。     

多头带绦虫的分类研究进展

对于寄生在中枢神经系统外的多头蚴,有人将其命名为格氏多头蚴,并且认为是区别于脑多头蚴的一个新的株或基因型;也有人将其命名为斯氏多头蚴或认为是脑多头蚴的同种异名。现就目前报道的寄生在绵羊中枢神经系统及山羊中枢神经系统外的多头带绦虫的分类现状进行简要综述,主要依据流行病学从传统形态学及现代分子生物学两方面进行阐述,为多头带绦虫的分类工作提供科学依据。     

羊多头蚴的诊断与治疗

作为一种典型的寄生虫病,羊多头蚴也被称作脑包虫、棘球蚴病,由多头蚴感染而诱发.基于此,本文将简单分析羊多头蚴的诊断要点,并深入探讨羊多头蚴的治疗方法选择.     

青海省牛羊多头蚴病流行情况调查

对1991—2005年青海省牛羊多头蚴流行情况进行了调查。结果表明:15年牛发病率平均为3.01%,死亡144头,致死率28.57%;羊发病率平均为1.44%,死亡23 272只,致死率为47.65%。发病畜主要是周岁羊,13岁牛。一年四季均可发病,春季为高发季节,并造成死亡。该病的发生呈散发,但也有较强的区域性,发病未见性别差异。     

山羊脑多头蚴形态学与cox1基因鉴定

为鉴定山羊脑多头蚴,通过对虫体进行形态学观察并采用PCR扩增虫体线粒体细胞色素C氧化酶亚基1(cox1)基因,将有效序列进行NCBI-Blast在线比对,利用MEGA6.0构建遗传系统进化树.结果 显示,山羊脑多头蚴cox1序列长度为446 bp,与多头带绦虫同源性达100％,系统进化分析显示分离株与多头带绦虫位于同一...     

山羊多头蚴的调查报告

笔者对寄生在福建省莆田县平海乡山羊体内的多头蚴进行囊包形态结构、虫体寄生特点和寄主发病症状进行了详细调查；并用寄生于山羊肌间和脑腔的多头蚴喂狗作感染试验，且对试验狗感染所得的线虫进行形态观察。经以上观察，笔者认为该地区山羊所感染的多头蚴为多头多头蚴，其成虫是多头带虫（Ｍ．ｍｕｌｔｉｃｅｐｓ）。     

一例羊脑多头蚴病的诊治报告

羊多头蚴病又称脑包虫病，是多头带绦虫的幼虫—脑多头蚴寄生于羊的脑和脊髓引起疾病。患病羊几乎是羔羊和2岁以内的年轻羊，山区发病率较高，严重影响养羊业的发展。2006年3月，我市一养羊户饲养的羊发生了本病，现将诊治情况阐述如下。     

A survey of Taenia multiceps coenurosis in Sardinian sheep

A survey was carried out to assess the occurrence of Coenurus cerebralis infection in Sardinian sheep. A prevalence of 0.35% was observed when 566 regularly slaughtered sheep were examined. However, in 120 sheep with suspected symptoms of coenurosis examined from November 2001 to October 2002, a total of 299 cerebral coenurosis lesions were observed with an incidence of 1% per year. Lesions were classified as migratory, cystic and secondary. Most migratory lesions were found in sheep aged 3-6 months. Cavitary lesions containing cysts in different developing stages were found with high incidence per year in sheep aged 7-12 months. Secondary lesions due to the development of Coenurus were most frequent in sheep aged 19-36 months. Most sheep were found infected in spring and in early summer, between March and June. Most lesions were located in the cortex. The mean number of protoscolices per cyst was 149 (range 10-370).     

Prevalence of Taenia multiceps in sheep in northern Jordan

From March 1996 to February 1997, slaughtered-sheeps' heads were examined for the metacestode of Taenia multiceps. Out of 451 sheep heads, only 12 (3%) were infested. All cysts but one were in the age group 0.5-2.5 years old, with prevalence of 10% (11 of 108). Eleven of 12 infested heads were found during the spring and autumn seasons. Out of 95 flocks involved in questionnaire survey unrelated to the abattoir survey, all the farmers reported the existence of the clinical signs known for coenurosis and described it in locally known names and diseases. Twenty-five of them had recent cases that were diagnosed by the veterinarian as coenurosis cases. Each flock reported one or two cases at the time of survey. A variety of clinical signs were reported from these cases (n = 42). These included circling (86%), head pressing (52%), blindness (29%) and paresis (40%). Coenurosis is a sheep-health problem and an important cause of sheep culling in Jordan.     

Immunization of sheep against the larval stage of Taenia multiceps

A trial with 200 ewes and 127 lambs showed that vaccination of lambs with 2 doses of Oncosphere Secretory Antigen (OSA) 4 weeks apart, at either 4-8 and 8-12 weeks or at 12-16 and 16-20 weeks of age, was equally effective in protecting them against infestation with the larval stage of Taenia multiceps. In this trial, the lambs of ewes, vaccinated with OSA when they were 90 and again 120 days pregnant, were as susceptible to infestation as the lambs of untreated control ewes. Another trial to provoke passive immunity in lambs gave inconclusive results. In a 3rd trial 2 doses of regular OSA and 2 doses of freeze-dried OSA protected all the lambs in each group, while a single dose of regular OSA protected 9 out of 10 lambs against cerebral lesions.     

多头带绦虫TPx基因的生物信息学简析

从患病绵羊脑内采集多头蚴原头节,提取总RNA,采用RT-PCR技术扩增多头带绦虫硫氧还蛋白过氧化物酶(TmTPx)基因,PCR产物连接到pMD18-T载体,转化至大肠埃希菌DH5α后测序,克隆到的TmTPx基因开放阅读框为591bp,编码196个氨基酸。运用DNASTAR、Rpsblast等软件对TmTPx基因进行生物信息学分析,能确定TmTPx的蛋白表面暴露区、蛋白骨架柔韧性、蛋白抗原指数、功能域等,利于从分子水平了解TmTPx的功能及多头带绦虫的生理代谢、进化分类。     

多头带绦虫蛋白激酶A催化亚基基因的克隆及原核表达

为鉴定多头带绦虫蛋白激酶A催化亚基（TmPKA-C）基因作为诊断抗原的潜能,本试验以多头带绦虫成虫RNA为模板,利用RT-PCR扩增TmPKA-C基因。将TmPKA-C基因片段连接至pET-30a原核表达载体进行原核表达,然后利用Western blotting和间接ELISA分析该蛋白的反应原性。结果表明,TmPKA-C基因开放阅读框的长度为1 032 bp,可编码343个氨基酸。TmPKA-C基因的表达产物主要以包涵体的形式存在,重组蛋白的分子质量大小约42 ku。Western blotting结果表明,该重组蛋白既能与自然感染脑多头蚴的绵羊血清发生特异性反应,又能与人工感染脑多头蚴不同时间段的绵羊血清发生特异性反应,表明该重组蛋白具有良好的反应原性。间接ELISA分析结果表明,脑多头蚴绵羊血清能与重组蛋白发生特异性反应,进一步证明该重组蛋白的反应原性良好。本研究为筛选脑多头蚴病诊断新抗原奠定了基础。     

多头带绦虫Tm16与Tm18抗原基因的克隆及原核表达

为原核表达Tml6和Tm18重组蛋白,本研究以自然感染羊源脑多头坳原头节基因组DNA为模板分别扩增Tml6和Tm 18杭原基因全基因片段,测序鉴定后合成其开放阅读框架DNA片段,将基因组序列中的内含子去除并对其稀有密码子进行改造优化,构建重组表达质粒pGEX-Tm 16和pGEX-Tml8.转化大肠杆菌BL21后以IPTG诱导表达,SDS-PAGE分析表达产物并进行纯化.结果显示:在大肠杆菌中表达出带有GST标签的大小约为39.6 ku和39.4 ku的重组蛋白,经谷胱甘肤琼脂糖树脂纯化得到高纯度的可溶性的GST-Tm 16及GST-Tml8重组蛋白,western blot分析表明重组蛋白均能够被兔抗GST单克隆杭体特异性识别.     

Cloning and Prokaryotic Expression of Protein Kinase A Catalytic Subunit Gene from Taenia multiceps

To study the potential of protein kinase A catalytic subunit of Taenia multiceps (TmPKA-C) gene as a diagnostic antigen, the TmPKA-C gene was amplified by RT-PCR from the total RNA of Taenia multiceps. The TmPKA-C gene fragment was ligated into prokaryotic expression vector pET-30a and transformed into Escherichia coli Transetta (DE3) strain, then the reactonogenicity of recombinant TmPKA-C protein was analyzed by Western blotting and indirect ELISA. The open reading frame of TmPKA-C gene was 1 032 bp, encoding 343 amino acids. The expression product of TmPKA-C gene mainly existed in the form of inclusion body, and the molecular weight of recombinant protein was about 42 ku. Western blotting analysis showed that the recombinant protein could react specifically with the sera from naturally infected sheep with coenurosis, and it could also have specific reaction with the sera of artificial infected sheep with coenurosis. The indirect ELISA analysis showed that the sera from the coenurosis of sheep could react specifically with the recombinant protein. These results proved that the recombinant protein had good reactionogenicity. This study laid a foundation for the search of new diagnostic antigens for coenurosis of sheep.     

广西部分地区猪流感病毒的血清学调查

为了解广西猪群中猪流感(SI)的流行情况,以猪流感病毒H3N2亚型作为诊断抗原,采用微量血凝抑制试验(HI)方法,对广西部分市(县)的猪群进行猪流感的血清学调查.在12个市(县)采集的1000份血清中,有10个市(县)检出H3N2抗体,共有257份血清呈H3N2抗体阳性,平均阳性率为25.7%.百色的阳性率最高,达到82.9%,贵港和南宁市郊两地没有检出阳性,其它9个市(县)的阳性率在13.0%～45.7%之间.结果表明我区猪群中很可能存在H3N2亚型猪流感感染,其中百色地区猪流感的感染率比较高.     

山东省鸡传染性贫血流行病学调查

本文对山东省12个地市58个鸡群传染性贫血的流行情况进行了调查,共检测了368份血清样品,蛋鸡群、肉鸡群和种鸡群的鸡传染性贫血病毒(CAV)ELISA抗体检出率分别为91.7%、77.6%和84.78%,平均抗体阳性率为83.2%,结果表明近几年山东省鸡群中CAV感染相当普遍,而且蛋鸡和种鸡抗体阳性率明显高于肉鸡群。