Comparative efficacy of diminazene diaceturate and diminazene aceturate for the treatment of babesiosis in horses

The present work was conducted to study the comparative efficacy of two anti-protozoan (babesicidal) drugs on the recovery and health of horses. A total of 80 horses, showing typical clinical symptoms of the disease, were selected for this study; the presence of babesiosis was confirmed through blood smear examination. These animals were divided into two groups i.e. A and B. Horses of Group A, were treated with diminazene diaceturate, while horses of Group B were treated with diminazene aceturate. Efficacy of the drugs was determined by the reversal of clinical signs and a negative blood smear examination. The efficacy of diminazene diaceturate was demonstrated to be 80% while diminazene aceturate was found to have 90% efficacy against babesiosis.     

鸡滑液囊支原体活疫苗(MS-H株)同时免疫对新支二联活疫苗免疫效力影响的调查

为了解新支二联活疫苗和鸡滑液囊支原体活疫苗之间有无相互影响,选用70只1日龄SPF鸡随机分为4组进行比对试验,其中:G1组20只,联合免疫新支二联苗活疫苗和鸡滑液囊支原体活疫苗(MS-H株);G2组20只,为新支二联活疫苗单独免疫组;G3组20只,为传染性支气管炎病毒(IBV)攻毒组;G4组10只,为空白对照组.免疫后...     

活菌载体疫苗的研究概述

活菌载体疫苗是指将病原菌特异性抗原基因片段插入已知细菌中,以提呈表达所编码的抗原。因其具有安全性好、生产费用低、易于构建多价疫苗、能激起持久的系统和黏膜免疫反应且兼具免疫佐剂作用等优势,已成为疫苗领域的研究热点。目前,对活菌载体的研究主要集中在沙门氏菌、牛分枝杆菌-卡介苗(BCG)、志贺菌、单核细胞增多性李斯特杆菌等,本文主要对这类载体及疫苗研究做一概述。     

耐热保护剂冻干活疫苗冷冻干燥工艺研究

以鸡新城疫、传染性支气管炎二联耐热保护剂冻干活疫苗的冷冻干燥试验为例,描述了冻干耐热保护剂冻干活疫苗时的工艺改进过程,获得了较佳冻干工艺曲线.     

Studies on failure of T strain live Babesia bovis vaccine

SUMMARY Field investigations of the protection afforded by the Australian live Babesia bovis vaccine used in the early 1990s (T strain) revealed inadequate vaccine-induced protection in certain herds. Vaccination/challenge trials using 207 experimental cattle were conducted to evaluate the protection afforded by T strain B bovis against field isolates from these herds. The trials investigated whether isolates that could ‘break-through’ T strain immunity were present in the field, the ability or inability of specific cattle to develop protective immunity after vaccination with T strain and the effect of attenuation and maintenance procedures on the immunogenicity of T strain. The results showed that B bovis parasites present early in the process of attenuation of T strain were more protective than those remaining late in the process. They also showed that cattle from properties experiencing vaccine failures were less protected by T strain vaccination than Bos taurus cattle randomly selected from the general population if vaccinated with highly attenuated T strain. A hypothesis is offered to explain these findings .     

A live Pasteurella haemolytica vaccine efficacy trial

A live Pasteurella haemolytica serotype 1 vaccine was used in an efficacy trial conducted on 100 lightweight feeder calves purchased from a Florida ranch. Forty-one calves were inoculated with the vaccine intradermally in the neck. Fifty-nine calves served as nonvaccinated controls. Fourteen days later, the calves were shipped to an order buyer in eastern Tennessee, where the calves were mixed with 60 local calves in a community sale barn for 72 hours. After 3 additional days, the calves were shipped to a research feedlot in Bushland, Tex. They remained in the feedlot for 56 days, and the test was concluded 76 days after vaccination. The P haemolytica vaccine had no significant effect on performance, morbidity, or mortality. There was no significant difference between the vaccinated and nonvaccinated calves in the number of times Pasteurella was isolated. The calves became seropositive to bovine viral diarrhea virus, respiratory syncytial virus, and infectious bovine rhinotracheitis (IBR) virus during the 76-day experiment. All calves initially were seropositive to parainfluenza-3 virus. A virulent outbreak of IBR occurred 30 days after the calves arrived at the feedlot. Before the onset of IBR, the isolation of P haemolytica serotype 1 from nasal turbinates was rare (2 of 500 nasal swabs). After the IBR outbreak, P haemolytica serotype 1 was isolated from 40 of 92 calves.     

山羊痘活疫苗临床免疫效果观察

1材料与方法 1.1主要试剂和器材山羊痘琼扩抗原和阳性血清、山羊痘野生强毒、山羊痘参考阳性抗原,兔抗山羊痘高免血清,山羊痘标准阴性血清,其他常见动物病血清以及山羊痘反向间接血凝诊断液由贵州大学动物医学院提供,山羊痘抗体检测也在贵州大学动物医学院进行.     

Use of live oocyst vaccine in the control of turkey coccidiosis: Effect on performance and intestinal morphology

An experiment was conducted to determine the effects of different coccidiosis-preventing programs on performance and intestinal morphology of commercial turkeys. Three hundred fifteen1-d-old female commercial cross turkey poults (British United Turkeys, BUT Big 9) were distributed into 3 treatments with 5 replicates of 21 birds each. Three programs were evaluated from 1 to 70 d of age, where program 1 had no anticoccidial drug and no vaccination against coccidiosis; program 2 had an anticoccidial drug (maduramycin 1%, 5 ppm); and program 3 had a vaccination (commercial vaccine, 4 species of Eimeria). All the groups were challenged with a dose of oocysts sporulated (20,000/bird) of 2 species of Eimeria at 21 d of age. In the growing phase (d 0–28), BW, BW gain, and FCR were significantly greater in treated groups compared with control group. In the fattening phase, the performance was not affected by treatments. Treatments and coccidiosis challenge had no significant effects on intestinal villus height. These observations support other reports that confirm live oocyst vaccination can be used effectively as a preventive against avian coccidiosis in commercially reared turkeys.     

甲硝唑对鸡传染性法氏囊病活疫苗免疫效果的影响

本研究观察了药物对传染性法氏囊病(IBD)活疫苗初次免疫和二次免疫的影响,结果显示高抗传染性法氏囊病病毒(IBDV)母源抗体雏鸡IBD活疫苗初次免疫前应用10～100毫克/千克甲硝唑和10毫克/千克左旋咪唑,在免疫鸡血清抗IBDV抗体水平均明显下降,甲硝唑用药鸡疫苗二次免疫后,血清抗IBDV抗体滴度明显高于未用药对照组.10毫克/千克左旋咪唑给药组疫苗二次免疫后鸡血清抗IBDV抗体滴度与未用药对照组无明显差别.     

小反刍兽疫活疫苗效力评价

以新疆天康畜牧生物技术股份有限公司生产的6批小反刍兽疫活疫苗为材料,对其进行最小免疫剂量、疫苗免疫期和疫苗保存期的研究。结果显示,该疫苗最小免疫剂量为103 TCID50/头份;免疫期至少为26个月,保存期为-20℃保存12个月。结果表明,该疫苗免疫原性好,对山羊和绵羊均可提供良好保护。     

含硒型牛副伤寒活疫苗保存期试验的研究

为了弄清含硒型牛副伤寒活疫苗及其专用稀释液的最佳保存条件及保存期,将四批含硒型牛副伤寒活疫苗(批号200001、200101、200102、200201)分别置-20℃、0～4℃、15～21℃保存不同时间后,检查其活菌率及其对豚鼠的免疫效力;同时用3批含硒型牛副伤寒活疫苗专用稀释液(批号200101、200201、200202)分别置室温(15～21℃)下保存不同时间后,检查其物理性状及灭菌状态.结果显示,这四批疫苗于-20℃保存24个月、0～4℃保存12个月、15～21℃保存2个月,活菌率均在80%以上,对豚鼠的免疫效力均合格;同时这三批含硒型牛副伤寒活疫苗专用稀释液于15～21℃保存12个月外观物理性状及无菌检验均合格.因此,含硒型牛副伤寒活疫苗保存期暂定为-20℃ 24个月、0～4℃ 12个月、15～21℃ 2个月;保存专用稀释液15～21℃(室温)保存期暂定为12个月.     

Prevention of experimental haemorrhagic septicaemia with a live vaccine

Pasteurella multocida serotype B:3,4 isolated from a fallow deer in England was used as a vaccine to prevent haemorrhagic septicaemia. The deer strain was less virulent for calves than typical serotype B:2 of haemorrhagic septicaemia strains. It elicited antibodies in cattle that protected mice against serotype B:2 infection. The live deer vaccine containing 2 X 10(7) viable organisms per dose was used to immunise calves. Six months after vaccination, five of six calves were protected against serotype B:2 challenge. Two calves challenged nine months after vaccination survived the same challenge. The live vaccine was more efficacious than an alum precipitated vaccine in protecting calves against B:2 challenge.     

新城疫活疫苗对控制鸡大肠杆菌病的效果观察

为了研究鸡新城疫活疫苗紧急免疫控制鸡大肠杆菌病的效果,通过大肠杆菌人工感染30日龄蛋鸡,待出现病症后,紧急免疫新城疫La-sota弱毒苗(Ⅳ系)4羽份.免疫后5～17 d,通过血凝抑制试验测定血清新城疫抗体水平,试验组新城疫抗体水平逐步提高,对照组抗体略有下降,免疫后17 d两组抗体水平差异显著(P＜0.05);对照组...     

Production and application of a live Salmonella gallinarum vaccine

The production and application of a freeze-dried Salmonella gallinarum vaccine are described in this report. The vaccine is stable when kept at 4 degrees C and a single injection elicits a good immunity for 2 months, though its effect gradually diminishes. Immunity is neither enhanced nor depressed by repeated injections of the live vaccine, and no interference effect was observed in experimentally infected chickens. Furazolidone therapy jeopardizes the immunogenicity of a live vaccine, but its effect can be countered by the administration of either an inactivated or a live vaccine when medication is commenced and this is followed by the application of live vaccine 6 days after cessation of medication.     

四价鸡球虫苗的免疫效果研究

在实验条件下观察自主研制的四价鸡球虫苗(LCV)对平养鸡的安全性和免疫保护效果.试验设空白对照组(NC)、攻虫对ECX(PC)和免疫组(VC)3个试验组,每组30只鸡.试验结果:空白对照组、攻虫对照组和免疫组的相对增重率依次为100%、84.77%和94.97%;免疫后(免疫后攻虫前)排卵囊高峰期间(免疫后5 d～10 d),免疫组平均OPG(即每克粪便申的卵囊数)为2.70 X 104,攻虫后排卵囊高峰期间(攻虫后6 d～13 d)攻虫对照组组平均OPG为2.32 X 106,高于免疫组(2.69 X 103)(P<0.01);攻虫后各组死亡率依次为0、10%和o;3个组的ACI(抗球虫指数)分别为200,0、154.77和184.97,免疫组试验效果最佳.结果表明,LCV安全性高、免疫保护效果理想.接种该疫苗后的鸡对较大剂量攻虫具有较强的抵抗力.