共查询到20条相似文献,搜索用时 93 毫秒
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1992年以来,福建省海水养殖虾类发病十分严重,同样的在育苗期内也出现虾病多,育苗成功率低的问题。为弄清此问题,现将我们近几年来对进境斑节对虾亲虾及育苗期内幼苗的疫病检疫、防治以及今后的防治对策分述如下,以供同仁参考。1材料和方法1.1材料1.1.1随机抽取近三年来捕自印尼、马来西亚、菲律宾、泰国等地深海区并经新加坡空运至厦门空港进境的斑节对虾亲虾、亲虾粪便和水体。1.1.2取自闽南地区育苗场内亲虾、育苗期内不同发育期的虾苗、病虾及水样。1.2方法1.2.五斑节对虾杆状病毒(MBV)的检测方法:1.2.1.l粪便中MB… 相似文献
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This experiment used carbodiimide get egg albumin synthesis of artificial antigens coupling neomycin,through square test to determine the best antigen packaged concentration and antibody dilution ratio,mass concentration logarithm of neomycin as abscissa,inhibition rate of antibody as the ordinate drawing standard curve,established ELISA fast detection techniques of neomycin in edible animal for import and export.Methods of recovery and specific test had been done in further research.Results showed that the test dilution ratio of optimum synthetic antigen was 1:200,the best dilution multiple of antibodies was 1:2 000, half inhibitory concentration(IC50) was 6.99 ng/mL;The minimum detection limit of the method was 40 ng/mL,and cross reaction rate of gentamicin, kanamycin, streptomycin,tobramycin,amikacin and dihydrostreptomycin were less than 0.1%,the average of recovery rate was 83.77%.The range of standarding curve in 1.5~40 ng/mL was linear,the correlation coefficient was 0.987,the equation of standarding curve was y=-37.66x+94.592;Compared with HPLC method,the operation was simple and fast.The test results showed that the enzyme-linked immunosorbent method of detecting neomycin was rapid,efficient and specific. 相似文献
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应用碳二亚胺法合成人工抗原得到卵清蛋白偶联新霉素,通过方阵试验确定最佳抗原包被浓度与抗体稀释倍数,以新霉素质量浓度对数为横坐标、抗体抑制率为纵坐标绘制标准曲线,建立了进出口食用动物中新霉素ELISA快速检测技术,并对建立的方法进行了回收率和特异性试验。结果表明,建立的检测方法包被人工合成抗原的最适稀释度为1:200,抗体的最佳稀释度为1:2 000,半数抑制浓度(IC50)为6.99 ng/mL;该方法的最低检测限达40 ng/mL,与庆大霉素、卡那霉素、链霉素、托普霉素、阿米卡星、双氢链霉素的交叉反应率均小于0.1%,平均加标回收率83.77%。标准曲线在1.5~40 ng/mL范围内是线性的,相关系数为0.987,标准曲线方程为y=-37.66x+94.592;与常规液相检测方法相比操作简单、快速。本研究建立了一种快速、高效、特异的新霉素ELISA检测方法。 相似文献
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利用抗兔出血症病毒(RHDV)的特异性单克隆抗体(McAb)建立了ELISA阻断试验用以检测血清抗体,并与血凝抑制(HI)和琼脂扩散试验(ID)进行了比较。结果ELISA阻断试验敏感性超过HI,更远胜于ID,具有灵敏、特异、准确等优点,适于大批血清样品的快速检测。 相似文献
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ELISA一步法快速检测牛奶中三聚氰胺的研究 总被引:1,自引:0,他引:1
在制备三聚氰胺单克隆抗体的基础上建立了一种用于检测牛奶中三聚氰胺含量的间接竞争酶联免疫检测方法,采用一步法操作,评估了方法的回收率、准确度、精密度和特异性,并利用液相色谱―串联质谱法进行了盲样确证试验。结果显示,该方法的线性检测范围为10~810 ng/mL,灵敏度为10 ng/mL,回收率为84.0%~90.0%,同类似物交叉反应率均小于0.1%。本试验建立的ELISA一步法能满足牛奶中三聚氰胺的检测要求。 相似文献
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WANG Li-zhen LUO Gui-fang ZHANG Yan-ping SHEN Yong WU Yuan-xiao SHI Yan CHEN Rui-ai HE Dong-sheng 《中国畜牧兽医》2017,44(2):377-383
Porcine epidemic diarrhea (PED) is an acute, highly contagious enteric disease of pigs. Porcine epidemic diarrhea virus (PEDV) is the causative agent of PED. PED has caused significant economic losses to the pig industry. In this study,the purified PEDV as the coating antigen, by optimizing the ELISA reaction conditions,the indirect ELISA antibody detection method was established. The optimized reaction conditions were as follows: Antigen working concentration was 20 μg/mL; Serum sample dilution was 1:500;It was coated at 4℃ overnight; The plates were blocked by 5% calf serum incubated at 37℃ for 1 h; The secondary antibody was diluted at 1:10 000,incubated at 37℃ for 1 h. It was judged as positive when the cutoff value D450 nm≥0.289,as negative when D450 nm≤0.236,and as suspicious between 0.289 and 0.236.It could not react with the positive sear of other six viruses such as porcine respiratory and reproductive syndrome virus,porcine circovirus 2, classical swine fever virus, porcine parvovirus,pseudo rabies virus and foot-and-mouth disease virus. The variation coefficient of repeated test was less than 10%.74 pig serum samples from Jiangsu, Jiangxi, Fujian and Guangdong were detected,and the positive rate was 84%.It indicated that this method could be used for PEDV epidemiological surveys and diagnosis in the future. 相似文献
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猪流行性腹泻(porcine epidemic diarrhea,PED)是由猪流行性腹泻病毒(porcine epidemic diarrhea virus,PEDV)引起的猪的一种急性、高度传染性肠道疾病,给养猪业造成了严重的经济损失。本研究以纯化的PEDV 为包被抗原,通过优化 ELISA 反应条件,建立了间接 ELISA 抗体检测方法,其反应条件为:抗原最佳包被浓度为 20 μg/mL,血清标准品最佳稀释度为 1:500,包被时间为4℃过夜,5%小牛血清37℃封闭1 h,二抗 1:10 000稀释,37℃作用1 h,抗体临界值为 D450 nm≥ 0.289判为阳性,D450 nm≤ 0.236判为阴性,介于二者之间为可疑。检测猪繁殖与呼吸综合征病毒、猪圆环病毒2型、猪瘟病毒、伪狂犬病毒、细小病毒和口蹄疫病毒标准血清抗体均为阴性,重复试验变异系数均小于10%,对江苏、江西、福建、广东地区74份猪血清样品进行检测,抗体阳性率达84%,表明该方法具有较好敏感性、特异性和重复性,可用于 PEDV 抗体检测和流行病学调查。 相似文献