Effect of exogenous estradiol on plasma concentrations of somatotropin, insulin-like growth factor-I, insulin-like growth factor binding protein activity, and metabolites in ovariectomized angus and braham cows

To determine the effect of breed and estradiol-17β on selected hormones and metabolites, ovariectomized (3 mo) Angus (n = 14) and Brahman (n = 12) cows were paired by age and body weight and randomly assigned as either nonimplanted controls (CON) or implanted with estradiol (E2) for 45 d. After Day 7 and through Day 42, plasma concentration of somatotropin was greater for E2 than CON cows (treatment X day, P < 0.05). During an intensive blood sampling on Day 36, E2 cows tended (P < 0.10) to have greater somatotropin pulse amplitudes than CON cows, but other parameters of somatotropin release were not affected (P > 0.10) by E2 treatment. The effect of breed was apparent on Day 36 as Brahman cows had greater (P < 0.05) somatotropin pulse amplitude, basal secretion, and mean concentration than Angus cows. Overall, plasma concentration of IGF-I was greater (P < 0.01) for E2 than CON cows (158.3 vs. 104.2 ng/ml) and was greater for Brahman than Angus cows (164.1 vs. 98.4 ng/ml). However, there was a trend (P < 0.10) for a treatment X breed X day interaction for IGF-I (i.e., the magnitude of increase in IGF-I concentration was greater in E2-Angus than E2-Brahman cows). After Day 7 and through Day 42, total plasma IGF binding protein (IGFBP) activity was greater (P < 0.01) for E2 than CON cows. Ligand blotting revealed at least five forms of IGFBP activity, and E2 cows had greater (P < 0.05) binding activity of IGFBP-3 and the 30- and 32-kDa IGFBP than CON cows. Brahman cows had greater (P < 0.05) IGFBP-3 and the 32-kDa IGFBP than Angus cows. After Day 14 and through Day 42, concentration of urea nitrogen (PUN) was greater (P < 0.001) for CON than E2 cows (treatment X day, P < 0.001). Brahman had greater (P < 0.01) PUN than Angus cows (16.6 vs. 14.2 mg/dl). Plasma concentration of glucose was greater (P < 0.01) for E2 than CON cows (78.9 vs. 76.4 mg/dl) but was not affected (P > 0.10) by breed. In summary, these data suggest that some, but not all, of the positive effects of estradiol on peripheral concentration of IGF-I and IGFBP activity can be attributed to increased somatotropin. Moreover, breed influenced basal and E2-induced secretion of somatotropin and IGF-I such that differences between Brahman and Angus cows in plasma IGF-I concentrations were abated within 3 wk of estradiol implantation. Thus, breed influences the metabolite and hormonal response of cattle to estrogenic implants.     

Maternal and reproductive performance of Brahman x Angus, Senepol x Angus, and Tuli x Angus cows in the subtropics

To determine the maternal and reproductive performance of F1 cows in the subtropics, 42 Brahman x Angus, 34 Senepol x Angus, and 50 Tuli x Angus cows were bred to Angus bulls to calve first and subsequently bred to Charolais bulls to calve as 3- to 8-yr-olds. Age at first calving did not differ among crossbred cows. Angus-sired calf birth weights were heavier (P < 0.01) from Senepol x Angus than either Brahman x Angus or Tuli x Angus cows. Weaning weights of Angus-sired calves were heavier (P < 0.01) from Brahman x Angus (213.5 kg) than either Senepol x Angus (194.9 kg) or Tuli x Angus (191.5 kg) cows. As 3- to 8-yr-old cows, calf birth weights were heavier (P < 0.05) from Senepol x Angus compared with Brahman x Angus but not Tuli x Angus cows. Weaning weights of Charolais-sired calves were heaviest (P < 0.05) from Brahman x Angus cows (268.9 kg), lightest from Tuli x Angus cows (233.4 kg), and intermediate from Senepol x Angus cows (245.0 kg). Calf crop born and calf crop weaned were lowest (P < 0.05) for Senepol x Angus cows (76.9 and 70.2%) and did not differ between Brahman x Angus (89.0 and 86.1%) and Tuli x Angus (94.7 and 86.5%) cows. Tuli x Angus cows tended (P < 0.10) to have a lower percentage of unassisted births and lower (P < 0.10) calf survival to weaning than Brahman x Angus cows but not Senepol x Angus cows. As 3- to 8-yr-olds, weaning weight per cow exposed was greatest (P < 0.05) for Brahman x Angus (234.2 kg), least (P < 0.05) for Senepol x Angus (173.0 kg), and intermediate (P < 0.05) for Tuli x Angus (209.1 kg) cows. Also as 3- to 8-yr-olds, efficiency (205-d calf weight per 100 kg of cow exposed) was similar for Brahman x Angus (42.2) and Tuli x Angus cows (40.7), and both were greater (P < 0.01) than for Senepol x Angus cows (33.8). These data indicate that, in the subtropics, maternal and reproductive performance of Tuli x Angus cows, but not Senepol x Angus cows, was comparable to Brahman x Angus cows, except for lower calf survivability and weaning weight.     

Composition and yield of milk from beef-type Bos taurus and Bos indicus X Bos taurus dams

Yield, butterfat, protein, lactose and solids-not-fat of milk from mature dams (n = 128) representing eight Bos taurus and Bos indicus X Bos taurus breed types were evaluated approximately 60, 105 and 150 d postpartum. Breed type was a significant source of variation in milk yield at each stage of lactation. Average 24-h milk yields (kg) were: Hereford, 7.3; Red Poll, 9.1; Hereford X Red Poll, 9.1; Red Poll X Hereford, 9.1; Angus X Hereford, 8.6; Angus X Charolais, 9.3; Brahman X Hereford, 7.3 and Brahman X Angus, 8.3. Daily yields of Brahman X Angus dams increased as lactation progressed, while production levels of other breed types remained approximately the same or declined. Hereford-Red Poll crosses showed significant heterosis in 24-h milk production and component yields at 150 d. Breed type effects also were significant for lactose yield throughout lactation. Sex of calf influenced (P less than .05) milk yield at 60 and 105 d postpartum and yield of protein and solids-not-fat at 105 d. Mastitis caused a reduction (P less than .01) in percentage of lactose but had no effect on milk yield. Residual correlations between yield traits and preweaning average daily gain were all positive and significant, with values ranging from .22 to .45. Breed type was a major source of variation in milk traits of beef-type Bos taurus and Bos indicus X Bos taurus dams.     

Effects of time of suckling during the solar day on duration of the postpartum anovulatory interval in Brahman x Hereford (F1) cows.

Previously published reports have indicated that postpartum anovulatory intervals can be markedly reduced and rebreeding performance enhanced in Bos taurus cows by eliminating nighttime suckling. We sought to confirm this hypothesis by examining the effects of day, nighttime, and ad libitum suckling on suckling behavior of calves, duration of the postpartum anovulatory interval, and pregnancy rates in 45 fall-calving Brahman x Hereford (F1) cows. Beginning on d 9 to 12 postpartum, calves were removed from lactating cows from 0700 to 1900 (Night-Suckled, n = 15) or from 1900 to 0700 (Day-Suckled, n = 15), or remained with their dams continuously (Ad Libitum-Suckled, n = 15). Cows in each group were maintained with fertile Angus bulls from d 10 postpartum until the first normal luteal phase or 100 d postpartum, whichever occurred first. Cows were observed for estrous behavior twice daily, and jugular blood samples were collected twice weekly for the determination of serum progesterone concentration. Mean number of suckling episodes per 24 h was greater (P < .0001) for the Ad Libitum-Suckled group than either Night- or Day-Suckled groups (5.9+/-.42 vs 3.8+/-.14, and 3.9+/-.32, respectively). Hourly analysis of suckling episodes in the Ad Libitum group indicated that they were not skewed toward a particular period, with suckling occurring at a periodicity of 4 to 6 h. Intervals to the first rise in progesterone > or = 1 ng/mL (32+/-2.5, 32+/-4.5, and 31+/-1.7 d, respectively), first normal luteal phase (38+/-3.1, 38+/-3.8, and 37+/-2.5 d, respectively), and first estrus (43+/-3.5, 40+/-3.9, and 36+/-1.1 d, respectively) did not differ (P > .05) among the three groups. Similarly, cumulative pregnancy rates within 100 d after calving did not differ (P > .05). These results in Bos indicus x Bos taurus (F1) cattle do not support the previous conclusions in Bos taurus that eliminating nighttime suckling reduces the postpartum anovulatory interval.     

Preweaning growth of F1 tropically adapted beef cattle breeds x Angus and reproductive performance of their Angus dams in arid rangeland

The objective of this study was to determine the preweaning performance of F1 Brahman (Bos indicus)-, Senepol (Bos taurus)-, and Tuli (Sanga)-Angus calves under semiarid south Texas conditions and to evaluate the reproductive performance of their Angus dams. Four hundred eighty-nine records collected over 4 yr were analyzed. The statistical model for performance traits included the effects of breed of sire, year, sex, age of dam, and breed of sire x year. Year effects were important (P < 0.05) for performance traits but could be explained, at least partially, by differences between years in rainfall patterns. Brahman F1 calves were 13% less (P < 0.05) vigorous at birth, 4.7 kg heavier (P < 0.05) at birth, 13.5 kg heavier (P < 0.05) at weaning, 0.25 units lower (P < 0.05) in body condition score (BCS) at weaning, and 1.75 units greater (P < 0.05) in frame score (scores of 1 to 9) at weaning than Tuli and Senepol F1 calves. Senepol F1 calves were intermediate (P < 0.05) between the Brahman and Tuli F1 calves for birth and weaning weight but had 11% more (P < 0.05) vigor at birth than the other two crossbreds. Tuli and Senepol F1 were similar (P > 0.05) in BCS and frame size at weaning. Males were 3.3 kg heavier (P = 0.12) at birth than females, especially for the F1 Brahman males that were 4.5 kg heavier (P < 0.05) than their counterparts. Brahman F1 weaned 19.9 kg heavier (P < 0.05) than the average of the other two F1 in the year of the greatest rainfall (1994), whereas the average advantage in other years was 11.4 kg. This difference gave rise to a breed of sire x year interaction (P < 0.003). Brahman F1 were heavier at every measurement and appeared to be later-maturing and more able to excel under good forage conditions than the other two F1 breed types; Senepol and Tuli F1 were similar (P > 0.05) in these respects but appeared to be more competitive in relative growth rate to the Brahman F1 calves in years of greater nutritional stress. Angus females were observed to have a relatively low reproductive rate and high apparent fetal loss at the first (27.5%) and second (19.2%) compared with the third or later pregnancy (11.2%). Angus females that gave birth to Brahman F1 calves had 20.1% lower (P < 0.05) pregnancy rates in the succeeding year than those that had given birth to the other two breeds.     

Effect of tropically adapted sire breeds on preweaning growth of F1 Angus calves and reproductive performance of their Angus dams

The objective of this study was to determine the effect of tropically adapted sire breeds on preweaning growth performance of F1 calves and on reproductive performance of their Angus dams. Angus (A) cows were bred in two consecutive years (1992 and 1993) by AI using semen from Brahman (B; Bos indicus; n = 10), Senepol (S; Bos taurus; n = 10), and Tuli (T; Sanga; n = 9) bulls. A total of 82 B x A, 85 S x A, and 91 T x A calves were born. The statistical model included the fixed effects of year, sire breed, calf sex, sire breed x calf sex, and cow parity and the random effect of sire within sire breed. Birth weight, weaning weight, 205-d adjusted weaning weight, ADG from birth to weaning, and hip height at weaning were greater (P < .001) for B x A calves than for S x A or T x A calves. Greater differences were detected between sexes for B x A than for S x A and T x A (for all traits sire breed x calf sex, P < .05). Sire breed affected (P < .01) the percentage of unassisted calvings (B x A, 87%; S x A, 98%; and T x A, 100%) and tended (P < .10) to affect the percentage of calves that survived until weaning (B x A, 90%; S x A, 94%; and T x A, 98%). Sire breed of calf did not affect (P > .10) length of gestation, and sire breed did not affect the interval from calving to first observed estrus or pregnancy in Angus dams. These results demonstrate that preweaning growth performance of B x A calves was greater than that of either S x A or T x A calves. However, use of Brahman sires on Angus dams led to calving problems and tended to reduce the percentage of calves that survived until weaning. Thus, heavier weaning weights of B x A calves would be an advantage for cow-calf producers marketing calves, but heavier birth weights and calving difficulty attributed to Brahman sires would be a disadvantage.     

Differences in copper and selenium metabolism between Angus (Bos taurus) and Brahman (Bos indicus) cattle

A 2-yr study was conducted at the Range Cattle Research and Education Center, University of Florida – Institute of Food and Agricultural Sciences (IFAS) (Ona, FL), to evaluate differences in the metabolism of Cu and Se of Angus (Bos taurus) and Brahman (Bos indicus) cattle. Thirty-two pregnant beef cows (n = 8 Brahman and 8 Angus/yr) were enrolled in the study in the first trimester of gestation. This study consisted of three phases: 1) restriction (day 0 to 90), 2) supplementation (day 91 to 150), and 3) calving. During all three phases, cows were individually fed and housed in partially covered drylot pens. During the restriction and supplementation phases, cows were provided a 1.5 kg/d of a grain-based concentrate supplement, which was fortified with flowers of S (50 g of supplemental S/cow daily; restriction phase) or Cu and Se (100 and 3 mg/d of Cu and Se, respectively; supplementation phase). Blood and liver samples were collected from all cows at 30 d intervals and from both cows and calves within 24 h of calving. Colostrum and milk samples were collected at calving and 7 d after birth. All data were analyzed using the MIXED procedure of SAS, where cow and calf were the experimental unit. During the restriction phase, a breed × day effect (P = 0.03) was observed where Brahman had greater liver Cu concentration than Angus cows in all sampling days. For liver Se concentration, a tendency (P = 0.07) for a breed effect was observed where Angus cows tended to have greater liver Se concentration than Brahman. During the supplementation phase, breed (P < 0.001) and day (P < 0.01) effects were observed, where Brahman cows had greater liver Cu concentration than Angus. For liver Se concentration, a day effect (P < 0.001) was observed, where liver Se concentration increased (P < 0.001) from day 90 to 120 and remained unchanged (P = 0.86) until day 150. At calving, no effects of breed (P = 0.34) were observed for liver Cu concentration of cows; however, Brahman calves tended (P = 0.09) to have greater liver Cu concentration than Angus calves. For Se liver concentration at calving, Angus cows tended (P = 0.07) to have greater liver Se concentration than Brahman cows; however, no breed differences (P = 0.70) were observed for liver Se concentration of calves at birth. In summary, substantial differences in multiple indicators of Cu and Se status were observed between Angus and Brahman cattle, implying that Angus and Brahman cattle possibly have different mechanisms to maintain adequate Cu and Se status.     

Output/input differences among nonpregnant, lactating Bos indicus-Bos taurus and Bos taurus-Bos taurus F1 cross cows

Nonpregnant F1 crossbred cows, progeny of either Hereford (H) or Angus (A) dams and sired by Brahman (Bm), Sahiwal (Sw), Pinzgauer (Pz), H, or A sires, were fed to maintain initial weight while rearing Charolais (C)-sired progeny for a period of 126 d in drylot commencing at about 48 d postpartum. Cow-calf pairs were assigned to equalize cow age, calf sex, and breed of cow's dam among three replicate pens of approximately 12 pairs each. Cows and calves were weighed every 2 wk and feed intake was adjusted to minimize change in cow weight. Metabolizable energy (ME) consumption for zero cow weight change was estimated by regression. Milk production was estimated by weight-suckle-weigh at 58, 85, 125, and 170 d of lactation. Calf gain (GAIN, kg) relative to cow weight (CWT1, kg) was higher (P less than .01) for calves from Bm-X (139.5/585) and Sw-X (132.2/534) than for calves from Pz-X (127.2/552) and HA-X (116.9/547) cows. Estimated mean daily production of milk was 7.40, 7.15, 7.28, and 6.37 kg for the Bm-X, Sw-X, Pz-X, and HA-X, respectively. Total cow ME intake (TMEcow) for breed groups ranked (P less than .05) with cow size and milk production, and calf creep-feed intake (FMEcalf) was inversely related to estimated milk intake. Proportion of total feed ME (TMEcow+calf) consumed by calves was higher (P less than .05) for HA-X cows (18%) than for the others (14%). Total efficiency of calf gain in weight (GAIN/TMEcow+calf) was 11% greater (P less than .05) for crossbred cows of Bos indicus X Bos taurus (Bm-X, Sw-X) than for Bos taurus X Bos taurus (Pz-X, HA-X) cows (35 vs 32 g/Mcal) in the 126-d lactation period.     

Metabolism and morphology of brown adipose tissue from Brahman and Angus newborn calves

The objective of this study was to compare adipocyte morphology and lipogenesis between breed types (Angus vs Brahman) in brown adipose tissue (BAT) and white adipose tissue (WAT) from newborn calves. The Brahman calves (n = 7) were born during the fall season, whereas the Angus calves were born in fall (n = 6) or the following spring (n = 4). At parturition, Brahman cows were lighter than fall Angus cows, but were heavier than spring Angus cows (P < .05). Birth weights and perirenal BAT weights were greater in spring-born, but not in fall-born Angus calves, than in Brahman calves (P < .05). Fall-born Angus BAT contained 63% more (P < .05) adipocytes/100 mg tissue and contained a greater proportion (P < .05) of adipocytes with mean diameters of 40 to 50 microm, and fewer adipocytes with diameters of 60 microm or greater, than Brahman BAT. Brahman BAT contained two-to-three times as many beta-receptors as Angus BAT (P < .05), although the dissociation constant (Kd) was not different between breed types. Mitochondria in Brahman BAT were primarily spherical, whereas Angus BAT mitochondria were elongated, and mitochondrial cross-sectional area tended (P = .08) to be greater in Brahman BAT than in Angus BAT. The mitochondrial uncoupling protein (UCP) mRNA concentration (per 10(6) cells) was greater in Brahman BAT than in BAT from fall-born Angus calves. Lipogenesis from acetate was greater in Angus BAT than in Brahman BAT (P < .05), and glucose and palmitate contributed a greater proportion of carbon to lipogenesis in Brahman BAT than in Angus BAT. These differences in lipogenesis between breed types were not observed in s.c. WAT. The WAT from both breed types contained adipocytes with distinct brown adipocyte morphology, suggesting an involution of BAT to WAT in utero. We conclude that differences in UCP gene expression cannot cause the greater peak thermogenesis of Angus calves; however, differences between breed types in lipid metabolism and(or) mitochondrial morphology may contribute to this phenomenon.     

Calving and weaning characteristics of Angus-, Gray Brahman-, Gir-, Indu-Brazil-, Nellore-, and Red Brahman-sired F1 calves

Calving and weaning data from crossbred calves sired by five Bos indicus breeds and one Bos taurus breed were evaluated. Data included calving and weaning records of F1 calves out of multiparous Hereford cows and sired by Angus, Gray Brahman, Gir, Indu-Brazil, Nellore, and Red Brahman bulls. At calving, Angus-sired calves had shorter gestations and lower (more desirable) calving ease scores and were smaller than Bos indicus-sired calves. Among the Bos indicus crosses, Gir calves had the shortest gestations, lowest calving ease scores, lightest birth weights (P less than .05), and smallest cannon bone lengths and heart girths. Nellore calves had the longest gestations (P less than .05) and largest heart girths. Calves by Indu-Brazil sires had the highest calving ease scores, highest birth weights (P less than .05), and greatest cannon bone lengths (P less than .05). Gray Brahman- and Red Brahman-sired calves were similar and intermediate for all calving characters. At weaning, Angus-sired calves had gained slightly faster than the Gir crosses and weighed more but were shorter at the hip than Gir crosses. Gir calves gained the least preweaning, weighed the least, and were shortest at weaning of the Bos indicus crosses. The Nellore and Indu-Brazil crosses were intermediate in preweaning gain and weaning weight to the Gir and the Red and Gray Brahman but were tallest at weaning. Gray Brahman and Red Brahman calves gained the most and were heaviest at weaning but were not as tall as the Nellore and Indu-Brazil.     

Comparison of F1 Bos indicus x Hereford cows in central Texas: I. Reproductive, maternal, and size traits.

Cows (n = 116) sired by Angus, Gray Brahman, Gir, Indu-Brazil, Nellore, and Red Brahman bulls and from Hereford dams were evaluated (when bred to the same breed of bull) for reproductive performance and cow weight and height and their calves (n = 1,161) were evaluated for birth and weaning weight in central Texas (temperate winters and subtropical summers). Cows were born from 1982 to 1985 and their calves were born from 1985 to 1999. Crossbred cows sired by Angus had a higher (P < 0.10) occurrence of calving difficulty than Gir, Indu-Brazil, Nellore, and Red Brahman crossbreds. Calves from Gir crossbreds had lighter (P < 0.10) birth weight (34.8 kg) than calves from Angus and Red Brahman crossbreds (39.4 and 37.2 kg). Calves from Angus crossbreds cows had lighter (P < 0.001) weaning weight (227.0 kg) than calves from Bos indicus crossbreds. Cows sired by Angus were lighter (P < 0.10) as mature cows (520.69 kg) than Gray Brahman, Indu-Brazil, and Red Brahman crossbreds (585.6, 571.9, and 577.6 kg, respectively). They also had smaller (P < 0.05) hip height (124.7 cm) than Bos indicus crossbreds. Mature cows sired by Indu-Brazil had greater (P < 0.05) hip height (138.3 cm) than Gir crossbreds (133.8 cm). Differences among sire breeds were noted within cow age categories (2 through 14 yr of age) for pregnancy rate, calf crop born, calf survival rate (as a trait of cow), and calf crop weaned. Angus and Indu-Brazil crossbred cows were lower (P < or = 0.10) than Gir and Nellore crossbreds for pregnancy rate, calf crop born, and(or) calf crop weaned at some, but not all, ages. Indu-Brazil crossbreds had a lower (P < or = 0.10) calf survival rate than most other breed groups at ages 11 to 14 yr. The results of this study indicate that Nellore and Gir crossbreds, but not Indu-Brazil, could perform as well or better than breeds traditionally used in cross-breeding programs of U.S. cow-calf production.     

Characterization of breeds of Bos indicus and Bos taurus cattle for maternal and individual traits

Angus, Boran and Red Poll sires were mated to Ankole, Boran and small East African Zebu (Zebu) females to produce the cows characterized in this study, which produced calves by Friesian, Brown Swiss, Simmental, Boran and Red Poll sires. Individual traits included cow parturition weight, cow weight at weaning, cow mean weight and cow weight change from parturition to weaning; maternal traits included calf crop born percentage, preweaning viability percentage, overall viability percentage, birth weight, weaning weight, weight at 12, 18 and 24 mo and calf weight weaned per cow exposed to breeding (productivity index). Cows by Angus and Red Poll sires significantly exceeded cows by Boran sires (all cows in this comparison had Ankole and Zebu dams) in weaning weight of progeny and in cow weight at parturition and at weaning. Cows by Red Poll sires significantly exceeded cows by Boran sires in birth weight of progeny. Progeny of cows by Boran sires significantly exceeded progeny of cows by Angus and Red Poll sires in 24-mo weight. Cows by Angus and Red Poll sires did not differ (P greater than .05) in any of the traits analyzed. Cows with Ankole and Boran dams significantly exceeded cows with Zebu dams (all cows in this comparison had Angus and Red Poll sires) in progeny weight at birth, 18 mo and 24 mo and in cow weight at parturition and at weaning. Cows with Boran dams significantly exceeded cows with Zebu dams in weaning and 12-mo weight of progeny and significantly exceeded cows with Ankole dams in weaning weight of progeny. The significantly heavier weaning weight and significantly lighter 24-mo weight of the 3/4 Bos taurus progeny of cows with Angus and Red Poll sires relative to the 1/2 Bos taurus progeny of cows with Boran sires suggest that cattle that are 1/2 Bos taurus breed composition have greater general adaptation than cattle with 3/4 Bos taurus breed composition in the postweaning nutritive and climatic environment under which this experiment was conducted. Significantly heavier weights of Angus and Red Poll crossbred cows relative to Boran crossbred cows (all breeds crossed on Ankole and Zebu cows) indicate that the two Bos taurus breeds exceed the Boran (Bos indicus) breed in additive direct genetic effects for size when they have general adaptation to the environment.     

The influence of tropical adaptation on plasma concentrations of insulin-like growth factor-I in purebred and crossbred beef cattle

In an effort to determine whether tropical adaptation influences circulating concentrations of the growth-related hormone IGF-I, 3-breed diallel matings were conducted using temperate Bos taurus (Angus), tropical Bos indicus (Brahman), and tropical Bos taurus (Romosinuano). Purebred Angus, Braham, and Romosinuano and crossbred Angus-Braham, Angus-Romosinuano, and Braham-Romosinuano heifers and steers were evaluated in 2 separate calf crops from 2003 and 2004. Blood samples were obtained from 10 heifers of each breed group (n = 90) for each year at weaning and on d 0 and 84 of postweaning trials. Samples were also taken from 10 steers of each breed group (n = 90) at weaning and on d 0 and 60 of individual finishing phase feeding trials for each year. Concentrations of IGF-I were determined by RIA. Analyses included effects of sire breed, dam breed, year of record, the age of the dam of the calf in years, and interactions. Age of calf in days was investigated as a linear and quadratic covariate. Separate analyses were conducted for steers and heifers. The direct effect of Angus was to reduce (P < 0.03) heifer concentrations of IGF-I at d 84 and in the repeated measures analysis. In the repeated measures analysis, the direct effect of Romosinuano was to increase concentrations of IGF-I (P = 0.01). Relative to the temperate Bos taurus breed, plasma concentrations of IGF-I were numerically greater in male and female tropically adapted breed groups.     

Preweaning efficiency for mature cows of breed crosses from tropically adapted Bos indicus and Bos taurus and unadapted Bos taurus breeds

Production data were collected on mature cows produced by mating Angus and Hereford (pooled AH), Brahman (Bh) and Boran (Br), and Tuli, a tropically adapted Bos taurus, sires by AI or natural service to Angus and Hereford cows. These cows were mated to Charolais bulls for the purpose of this study. Within each sire breed of cow, cows were assigned randomly to one of three feeding rates, 49 or 76 g of DMI/ BW0.75 or ad libitum (10 to 12 cows/feeding rate group), with weekly individual animal feed consumption recorded. Lactation yields were recorded via the weigh-suckle-weigh protocol at approximately 14, 28, 56, 84, 112, 140, 168, and 196 d postpartum for each cow/calf pair. Means for milk yield at peak lactation, total milk yields, calf birth weight, age-adjusted weaning weights, preweaning daily gain, and feed efficiency were estimated. Peak yield (kg/d) for Bh (10.3 +/- 0.36) was greater (P < 0.05) than for Tuli (9.0 +/- 0.31). Total yield (kg, 212 d) for Bh (1802 +/- 68) was greater (P < 0.05) than for Tuli (1532 +/- 59). Birth weight of AH (44 +/- 0.9) was heavier than for Bh and Br (P < 0.05). Preweaning daily calf gain (g/d) and adjusted weaning weight (kg) of Bh (813 +/- 28, 212 +/- 6.1) and Br (766 +/- 24, 202 +/- 5.1) differed (P < 0.05) from AH (589 +/- 24, 169 +/- 5.2) and Tuli (634 +/- 24, 176 +/- 5.3). Efficiency estimates (grams of adjusted weaning weight/kilograms DMI of the cow) for Bh (88 +/- 2.5) and Br (85 +/- 2.1) exceeded (P < 0.05) those for Tuli (74 +/- 2.1) and AH (73 +/- 2.1). Bos indicus breed crosses exhibited greater peak and total yield, lower birth weight, greater daily gain and adjusted weaning weight, and higher feed efficiency than did Bos taurus breed crosses (P < 0.05). Total yield, daily gain, adjusted weaning weight, and feed efficiency were higher (P < 0.05) for cows sired by bulls from tropically adapted breeds, and the peak yield was less (P < 0.10). Tuli exhibited lower total yield and birth weight than did Angus/Hereford (P < 0.05). The efficiency of crossbred Tuli cows did not differ from Angus/Hereford F1 females, but neither equaled the efficiency of crossbred cows produced using Bos indicus breeds.     

Metabolic characteristics of multiparous Angus and Brahman cows grazing in the Chihuahuan Desert

Understanding metabolic differences between breeds of cattle is important when selecting for production in semiarid environments. Spring-calving multiparous Angus and Brahman cows (n = 8/breed) grazing in the Chihuahuan Desert were used to evaluate metabolic hormone status in February (i.e., 3rd trimester of pregnancy), May (i.e., early lactation), and September (i.e., late lactation) 2000. Crude protein in ruminal digesta collected from grazing companion ruminally cannulated cows during these months was 5.6, 6.0, and 10.3%, respectively. Angus cows were heavier (P < 0.01) than Brahman cows across months. Body condition scores among breed groups were 4.4, 3.6, and 4.6 in February, May, and September with Brahman cows tending (P < 0.10) to have greater body condition than Angus cows in May. Brahman cows tended to have greater fecal output per 100 kg BW than Angus cows in February (0.9 > 0.7 +/- 0.1, P < 0.10). Brahman cows had greater serum concentrations of leptin than Angus cows in September (1.8 > 0.70 +/- 0.1 ng/mL; P < 0.05), and serum concentrations of insulin were greater (P < 0.01) in Brahman than Angus cows throughout the study. Brahman cows also had greater (P < 0.01) serum concentrations of glucose during February and May than Angus cows. Serum concentrations of triiodothyronine were greatest (P < 0.01) during September and lowest during February and May in both breed groups and were greater (P < 0.01) in Brahman than in Angus cows in February, May, and September. Pregnancy rate and 205-d adjusted weaning weights were similar (P > or = 0.46) among breed groups. Results suggest that Brahman and Angus cows are sensitive to the seasonal dynamics of forage quality in the Chihuahuan Desert. Brahman cattle appear to have greater concentrations of metabolic hormones and metabolites than Angus cows in this environment, but Angus cows experience greater fluctuations in BW.     

Cow/calf preweaning efficiency of Nellore and Bos taurus x Bos indicus crosses

The objectives of this study were to determine if percentage Bos taurus (0 or 50%) of the cow had an effect on ME requirements and milk production, and to compare cow/calf efficiency among 3 mating systems. Metabolizable energy requirements were estimated during a feeding trial that encompassed a gestation and lactation feeding trial for each of 2 groups of cows. Cows were 0 or 50% Bos taurus (100 or 50% Nellore) breed type: Nellore cows (NL; n = 10) mated to Nellore bulls, NL cows (n = 9) mated to Angus bulls, Angus x Nellore (ANL; n = 10) and Simmental x Nellore (SNL; n = 10) cows mated to Canchim (5/8 Charolais 3/8 Zebu) bulls. Cows were individually fed a total mixed diet that contained 11.3% CP and 2.23 Mcal of ME/kg of DM. At 14-d intervals, cows and calves were weighed and the amount of DM was adjusted to keep shrunk BW and BCS of cows constant. Beginning at 38 d of age, corn silage was available to calves ad libitum. Milk production at 42, 98, 126, and 180 d postpartum was measured using the weigh-suckle-weigh technique. At 190 d of age, calves were slaughtered and body composition estimated using 9-10-11th-rib section to obtain energy deposition. Regression of BW change on daily ME intake (MEI) was used to estimate MEI at zero BW change. Increase in percentage Bos taurus had a significant effect on daily ME requirements (Mcal/d) during pregnancy (P < 0.01) and lactation (P < 0.01). Percentage Bos taurus had a positive linear effect on maintenance requirements of pregnant (P = 0.07) and lactating (P < 0.01) cows; during pregnancy, the ME requirements were 91 and 86% of those in lactation (131 +/- 3.5 vs. 145 +/- 3.4 Mcal x kg(-0.75) x d(-1)) for the 0 and 50% B. taurus groups, respectively. The 50% B. taurus cows, ANL and SNL, suckling crossbred calves had greater total MEI (4,319 +/- 61 Mcal; P < 0.01) than 0% B. taurus cows suckling NL (3,484 +/- 86 Mcal) or ANL calves (3,600 +/- 91 Mcal). The 0% B. taurus cows suckling ANL calves were more efficient (45.3 +/- 1.6 g/Mcal; P = 0.03) than straightbred NL (35.1 +/- 1.5 g/Mcal) and ANL or SNL pairs (41.0 +/- 1.0 g/Mcal). Under the conditions of this study, crossbreeding improved cow/ calf efficiency and showed an advantage for cows that have lower energy requirements.     

Growth curves of crossbred cows sired by Hereford, Angus, Belgian Blue, Brahman, Boran, and Tuli bulls, and the fraction of mature body weight and height at puberty

The objective of this study was to evaluate the growth curves of females to determine if mature size and relative rates of maturation among breeds differed. Body weight and hip height data were fitted to the nonlinear function BW = f(age) = A - Be(k×age), where A is an estimate of mature BW and k determines the rate that BW or height moves from B to A. Cows represented progeny from 28 Hereford, 38 Angus, 25 Belgian Blue, 34 Brahman, 8 Boran, and 9 Tuli sires. Bulls from these breeds were mated by AI to Angus, Hereford, and MARC III composite (1/4 Angus, 1/4 Hereford, 1/4 Red Poll, and 1/4 Pinzgauer) cows to produce calves in 1992, 1993, and 1994. These matings resulted in 516 mature cows whose growth curves were subsequently evaluated. Hereford-sired cows tended to have heavier mature BW, as estimated by parameter A, than Angus- (P=0.09) and Brahman-sired cows (P=0.06), and were heavier than the other breeds (P < 0.001). Angus-sired cows were heavier than Boran- (P < 0.001) and Tuli-sired cows (P < 0.001), and tended to be heavier than Belgian Blue-sired cows (P=0.097). Angus-sired cows did not differ from Brahman-sired cows (P=0.94). Brahman-sired cows had a heavier mature BW than Boran- (P < 0.001), Tuli- (P < 0.001), and Belgian Blue-sired cows (P < 0.04). Angus-sired cows matured faster (k) than cows sired by Hereford (P=0.03), Brahman (P < 0.001), Boran (P=0.03), and Tuli (P < 0.001) sires, but did not differ from Belgian Blue-sired (P=0.13) cows. Brahman-sired cows took longer to mature than Boran- (P=0.03) or Belgian Blue-sired cows (P=0.003). Belgian Blue-sired cows were faster maturing than Tuli-sired cows (P=0.02). Brahman-sired cows had reached a greater proportion of their mature BW at puberty than had Hereford- (P < 0.001), Tuli- (P=0.003), and Belgian Blue-sired cows (P=0.001). Boran-sired cows tended to have reached a greater proportion of their mature BW at puberty than had Angus-sired cows (P=0.09), and had reached a greater proportion of their mature BW at puberty than had Hereford- (P < 0.001), Tuli- (P < 0.001), and Belgian Blue-sired cows (P < 0.001). Within species of cattle, the relative range in proportion of mature BW at puberty (Bos taurus 0.56 through 0.58, and Bos indicus 0.60) was highly conserved, suggesting that proportion of mature BW is a more robust predictor of age at puberty across breeds than is absolute weight or age.     

Effects of induced hypothyroidism on ovarian response to superovulation in Brahman (Bos indicus) cows.

To evaluate the effects of hypothyroidism on ovarian function, multiparous, nonlactating Brahman cows (n = 18) were assigned randomly to dietary treatments containing either 0 (C; n = 9) or 4 mg x kg BW(-1) x d(-1) 6-n-propyl-2-thiouracil (PTU; n = 9), to suppress thyroid function, in the feed concentrate. Weekly changes in BW and body condition score (BCS) were recorded. Dietary treatments began on d 10 of the estrous cycle. Ten days after the first treatment estrus, all cows received daily i.m. injections of 25 IU of porcine FSH over a 3-d period. Seven days after AI, embryos were collected nonsurgically, and the ovaries were removed via midflank laparotomy. Based on thyroxine (T4) concentrations after 49 d of treatment, five cows were hypothyroid (H-PTU) and four were partially suppressed (P-PTU). Cows in the PTU group had greater (P<.01) ADG, (P<.05) ovarian weights, and numbers of large (> or =8 mm) (P<.05) follicles. Cows in the PTU group had lower embryo recovery rate (P<.001), fertilization rate (P<.001), and percentage of blastocysts (P<.1) than C cows. The H-PTU cows had greater numbers of luteinized follicles (P<.06), greater concentrations of progesterone (P4) in the follicular fluid at all size categories (P<.1), and greater numbers of corpora lutea (P<.05) than C cows. The ratio of luteal to serum P4 on d 7 was greater (P<.05) in hypothyroid cows. Induced hypothyroidism improved weight gain and BCS, increased ovarian response to FSH, and affected ovulation, fertility, and P4 secretion in superovulated Brahman cows.     

Growth, puberty, and carcass characteristics of Brahman-, Senepol-, and Tuli-sired F1 Angus bulls

Postweaning growth, sexual development, libido, and carcass data were collected from two consecutive calf crops using 31 Brahman x Angus (B x A), 41 Senepol x Angus (S x A), and 38 Tuli x Angus (T x A) F1 bulls. Following weaning (by mid-September) and preconditioning, at the start of the study (late September) bulls were fed concentrate (three times each week at a rate equivalent to 4.5 kg/d) on bahiagrass pasture for approximately 250 d. At the start of the study and at 28-d intervals, BW, hip height, and scrotal circumference (SC) were measured. Concurrently at 28-d intervals, when the SC of a bull was > or = 23 cm, semen collection was attempted using electroejaculation. Ejaculates were evaluated for presence of first spermatozoa (FS), 50 x 10(6) sperm with at least 10% motility (PU), and 500 x 10(6) sperm with at least 50% motility (PP). After all bulls reached PP they were subjected to two libido tests. Carcass data were collected on all bulls (n = 110) and Warner-Bratzler shear (WBS) force values were assessed on a subset (n = 80). For both years, B x A bulls were heavier (P < 0.05) and taller (P < 0.05) than S x A and T x A bulls at the start and end of the study. However, breed type did not influence (P > 0.10) gain in BW or hip height during the study. Scrotal circumference of T x A bulls was larger (P < 0.05) than that of B x A or S x A bulls at the start of the study, but there was no effect (P > 0.10) of breed type by the end of the study. At PU and PP, B x A bulls were older (P < 0.05), heavier (P < 0.05), and taller (P < 0.05) and had larger (P < 0.05) SC than S x A and T x A bulls. Tuli x Angus bulls were younger (P < 0.05) than S x A bulls at PU and PP but had similar SC. Libido scores tended (P < 0.10) to be lower for B x A than for S x A and T x A bulls. Breed type affected (P < 0.05) carcass traits; B x A bulls had the heaviest (P < 0.05) hot carcass weight, greatest (P < 0.05) dressing percentage, larger (P < 0.05) longissimus muscle area than S x A bulls, and higher (P < 0.05) USDA yield grade than T x A bulls but greatest (P < 0.05) WBS force values. Breed type did not affect (P > 0.10) USDA quality grade. In conclusion, tropically adapted F1 bulls produced from Senepol (Bos taurus) and Tuli (Sanga) sires bred to Angus cows in Florida had lighter BW, shorter hip heights, and smaller carcasses than those from Brahman sires but reached puberty earlier and had higher libido scores and lower WBS force values.     

Physiological responses of Bos taurus and Bos indicus cattle to prolonged, continuous heat and humidity

Two experiments were conducted to investigate the physiological responses of Bos taurus (Angus cross, n = 6) and Bos indicus (Brahman, n = 6) cattle to prolonged heat and humidity, as can occur during live export by sea. Each experiment was carried out in climate-controlled rooms, where heifers were exposed to 15 d of sustained heat and humidity. The treatment was designed to be representative of a long-haul, live-export voyage leaving a southern Australian winter and traveling to a Middle Eastern summer. Wet bulb temperature (WBT) was used to give a combined measure of dry bulb temperature and relative humidity and was increased over several days, culminating in 5 d at 32 degrees C WBT between d 7 and 11. By d 11, the respiratory rate and core body temperature increased (P < 0.001) compared with values at lower ambient temperature on d 1 and 2 when climate-controlled rooms were not operating. Feed intake of Bos taurus was reduced (P < 0.001) by d 11, whereas that of Bos indicus did not change (P = 0.14). Despite no diurnal variation in climatic conditions, core body temperature of both Bos taurus and Bos indicus continued to show a circadian amplitude of approximately 1 degrees C throughout the hottest period. This amplitude increased during the recovery period after heat was removed (up to 1.8 degrees C for Bos indicus and 1.6 degrees C for Bos taurus). Water intake for both Bos taurus and Bos indicus increased when WBT increased (P < 0.01 on d 11). Significant acid-base and blood electrolyte imbalances occurred in both Bos taurus and Bos indicus, with changes in Bos taurus being more substantial and prolonged. The increase in respiratory rate coincided with a decrease in the partial pressures of carbon dioxide and bicarbonate in venous blood. However, during the hottest period, average daily venous blood pH remained unchanged. When the heat load was reduced after d 11, the blood pH decreased, indicating metabolic acidosis. Blood pH declined from 7.44 to 7.36 for Bos taurus (P < 0.001) and from 7.44 to 7.38 for Bos indicus (P < 0.001). Other parameters measured include heart rate; packed cell volume; plasma and urine Na, K, and Cl; urine pH; and specific gravity. Our results suggest that Bos taurus cattle experience significant physiological changes during exposure to prolonged and continuous high heat and humidity, with alterations persisting for some days after the heat-stress conditions subside. Bos indicus experience similar but less pronounced physiological changes.