Smallholder cattle farmers’ breeding practices and trait preferences in a tropical Guinea savanna agro-ecological zone

The present study was embarked upon to determine the breeding practices and choice of selection and breeding traits of cattle farmers in Nasarawa State and the Federal Capital Territory (FCT), Abuja, Nigeria. A total of 180 smallholder cattle herders were randomly sampled. However, analysis of data was restricted to 144 herders (81 from FCT and 63 from Nasarawa State). Primary data were collected through individual structured questionnaire administration. Chi-square (χ2) statistics were used to compare categorical variables. Arithmetic means of continuous variables between locations were tested using T test. Rank means were also calculated for within- and between-location comparisons of the preference traits. Chi-square automatic interaction detection (CHAID) algorithm was used to determine the influence of socio-economic parameters on the traits of preference. Access to credit, personal savings, and type of landholding were significantly (P?<?0.05) different between the two locations with higher values recorded for farmers in FCT. However, farmers in Nasarawa State had significantly higher land expanse (2.28?±?0.19 versus 1.29?±?0.09). The production objectives were rated similar between locations. The number of foundation stock differed significantly between farmers in the two locations with higher mean recorded in Nasarawa (7.37?±?0.37) compared to FCT (5.18?±?0.19). However, mean herd size was higher (34.60?±?2.58 versus 27.63?±?2.00; P?<?0.05) in FCT than Nasarawa. Among the productivity indices, only average age at first parturition and average calving interval were significantly (P?<?0.05) influenced by location. Within the FCT, calving interval was the trait of utmost importance in selecting the breeding stock, followed by milk yield, disease resistance, and body size. In Nasarawa State, calving interval, disease resistance, milk yield, body size, and survival were ranked similarly (P??>0.05). Across the two locations, body conformation (P?<?0.05) and calving interval (P?<?0.01) were more highly rated in FCT than Nasarawa State, while heat tolerance was less rated (P?<?0.05) in the former. All the traits of preference were influenced by socio-economic factors, and could be exploited in future breeding plans to boost cattle production. A new livestock census in the study area is also advocated to reflect the current reality as regards cattle distribution.

     

“Fear”, head shaking and production in five populations of caged chickens

1. Differences in “ fear”, head shaking and production traits were found among lines of chickens maintained in individual 0–46 × 0.30 × 0.46 m cages.

2. The differences among lines for “ fear “ and head shaking were consistent across three ages.

3. Although differences in “ fear “ and head shaking existed among lines for which there were large differences in production traits, the relationships within lines of production traits with modest “ fearfulness “ and head shaking were not important.     

Herd characteristics influence farmers’ preferences for trait improvements in Danish Red and Danish Jersey cows

The aim of this study was to characterize preferences of farmers for breeding goal traits with Danish Red (DR) or Danish Jersey (DJ) cows. A breed-specific survey was established to characterize farmers’ preferences for improvements in 10 traits, by means of pairwise rankings using the online software 1000Minds. These pairwise rankings were based on equal economic worth of trait improvements. The DR survey was filled in by 87 farmers and the DJ survey by 76 farmers. Both DR and DJ farmers gave the highest preference to improvements in mastitis, and the lowest to calving difficulty. By means of a cluster analysis, three distinct clusters of farmers were identified per breed. Comparisons of herd characteristics between clusters suggest that farmers choose to improve traits that are problematic in their herds. This study shows that heterogeneity exists in farmers’ preferences for trait improvements and that herd characteristics influence these preferences in DR and DJ.     

Participatory definition of trait preferences and breeding practices of goats in Aba’ala,Afar region: as input for designing genetic improvement program

Tropical Animal Health and Production - A farmer participatory approach was used to identify trait preferences and breeding practices for Afar goat population in Aba’ala district, Afar...     

Farmers’ breeding practices and traits of economic importance for indigenous chicken in RWANDA

Data on breeding practices and traits of economic importance for the indigenous chicken (IC) were collected through personal interviews using structured questionnaires and direct observations of chicken management practices. The study was conducted from November 2015 to January 2016 in Rwamagana, Rulindo, Ruhango, Kicukiro and Muhanga districts of Rwanda. Data were collected and analysed through computation of indices, which represented a weighted average of all rankings of a specific trait. Spearman’s non-parametric rank correlation was calculated for ranking of traits of economic importance to indicate the directional effects. The results on chicken ecotypes and their attributes showed that prolificacy, mature weight, disease tolerance, egg number and heat tolerance were highly preferred. The dwarf ecotype was most abundantly reared (38.84%) and considered to be significantly smaller and to have poorer growth rate, but to have better prolificacy than other indigenous chicken ecotypes. Selection of breeding cock and hen was based on disease tolerance, body weight at sexual maturity, body size and growth rate. In addition, for hen, mothering ability and egg fertility (Fer) were considered. Indices for the traits perceived by farmers as of primary economic importance were egg yield (0.093), disease tolerance (0.091), high growth rate (0.089), prolificacy (0.088), high body weight (0.087) and egg fertility (0.083). The most important traits considered by the marketers were body weight (BW), disease tolerance (Dtol), plumage colour (Pcol), egg yolk colour (EYC), meat quality (MQ), growth rate (GR) and egg yield (EY) whereas for consumers, meat quality, egg yolk colour, egg yield, body weight and growth rate were considered. Among traits perceived as important by farmers, a positive and significant correlation was found between BW and GR and Fer. Correlation was moderate for BW and prolificacy, drought tolerance (Drtol), Dtol and EYC. BW was negatively correlated with temperament (Temp), heat tolerance, Pcol and egg shell colour (ESC). Regarding marketers and consumers’ preference rank correlation, positive and significant correlation was between BW and GR and MQ. As such, appropriate ecotypes (indigenous chicken) which have these characteristics need to be identified and utilised more based on their performance and adaption to the environment conditions to ensure efficient IC production.     

Effects of structure and xylanase treatment of brewers’ spent grain on performance and nutrient availability in broiler chickens

1.?A factorial (2 × 3) feeding trial was set up to investigate the effects of coarse or finely ground brewers’ spent grain (BSG) and xylanase treatment, either with no xylanase, top-dressed with xylanase or pre-treated with xylanase.

2.?The experimental diets shared the same basal formulation and were fed to male broiler chickens (Ross 308) housed in individual cages from 12 to 29 d of age.

3.?Xylanase pre-treatment reduced the dietary concentration of arabinoxylan by 15–30%. Pellet durability increased when BSG was ground.

4.?Feed utilisation was significantly higher (6%) when the birds were given coarse BSG rather than ground BSG, whereas there was no significant effect of enzyme treatment. Apparent metabolisable energy was unaffected by the dietary treatments.

5.?The overall starch digestibility was high (99%), with no dietary differences, whereas ileal protein digestibility was low (57%). Xylanase top-dressing tended to improve ileal protein digestibility but, in general, xylanase treatment had no major effect on overall performance in male broilers given diets with BSG.     

Investigation of Marek’s disease virus from chickens in central Ethiopia

Marek’s disease (MD) is a lymphoproliferative and neuropathic disease of domestic chickens and less commonly, turkeys and quails, caused by a highly contagious, cell-associated, oncogenic herpesvirus. In Ethiopia, MD is believed to be introduced with importation of exotic and crossbred to improve the poultry production and has been reported to be a potential threat to the poultry sector both in backyard and commercial farming systems. This study was aimed at isolation and molecular analysis of MD virus isolates circulating in chicken population in the central part of Ethiopia where commercial farms are populated. From September 2013 to January 2014, clinical and post-mortem examination were conducted on diseased chickens suspected of MD virus infection. Representative spleen and feather follicle samples were collected following sterile procedure, and infectious virus isolation was performed using primary chicken fibroblast cell culture. Cell culture inoculated with suspension of pathological samples developed characteristic MD virus cytopathic effect of rounding of the cells and small plaques. Further analysis of the virus was conducted by conventional PCR amplifying the ICP4 gene fragment from eleven tissue samples using MD virus specific primers. PCR products were further sequenced and analyzed. Nucleotide sequence similarity search of the local isolates resulted a high degree of sequence similarity with Gallid Herpes virus type 2 strain (Marek’s disease virus type 1, JN034558). To our knowledge, the present study is the first report conducted on virus isolation and molecular characterization of MD virus isolates circulated in Ethiopia. Eleven ICP4-like gene fragment (318 bp) sequences generated in the present study were uploaded in the public database (KU842366–76). Further research on virus isolation, genetic characterization, and infection dynamics is recommended targeting chickens of all age groups reared in different agro-ecological zones under different production system.

     

Farmers’ perceptions on trypanosomosis and trypanotolerance character of the taurine Sheko

In the humid and subhumid tropics, trypanosomosis is an economically important zoonotic protozoan disease of the commonly kept farm animal species and their wild relatives. For example, more than 20% of the humid western and southwestern Ethiopia, which is home to more than 14 million heads of cattle, is under varying levels of trypanosomosis risk. Our study was, therefore, initiated to document farmers’ perception on trypanosomosis and Sheko’s trypanotolerance character. Our findings showed that trypanosomosis was the most frequently reported cattle disease in the Bench Maji Zone. Accordingly, 76.7% of the farmers reported the epidemiological importance of trypanosomosis, and they also noted that trypanosomosis on average accounted for 63.0% of annualized cattle death. The reported signs of trypanosomosis and trypanotolerance indicators were consistent with literature reports. Moreover, 66.7% of the farmers reported Sheko’s trypanotolerance character. In the course of time, smallholder farmers have developed ethnoveterinary practices that are mainly used to prevent the landing of vector flies on the animal. Wet and warm seasons of the year, i.e., spring and, to some extent, the beginning of summer and autumn, were reported as peak periods of trypanosomosis risk. Therefore, this showed the need for incorporating farmers’ knowledge in trypanosomosis control programs.     

Growth,carcase composition and profitability of meat chickens given pellets,mash or free‐choice diet

1. Equal numbers of 21‐d‐old male and female meat chickens were given their dietary ingredients either mixed and presented as pellets or mash, or as separate ingredients presented on a free‐choice basis, to determine whether presentation method caused any differences in growth, carcase composition or profitability to 42 d of age.

2. The proportions of ingredients eaten by the chickens given a free choice varied widely during the first 56 h of the trial. Thereafter, the proportions did not vary widely and birds given the free choice ate approximately ½ of their intake as whole wheat, ? of their intake as concentrate (high protein meals plus vitamin and mineral premixes), and 1/7 of their intake as whole sorghum each week for the three‐week trial period.

3. The nutrient composition of the free‐choice diet selected by the birds over the trial period averaged 12.09 MJ ME/kg feed, 210 g/kg crude protein, 14 g/kg calcium and 7 g/kg phosphorus.

4. At the end of the trial the average live body weights of the birds differed significantly according to method of feeding, in the descending order of pellets > mash > free choice.

5. The empty gizzard weight of birds differed significantly according to the method of feeding, in the descending order of free choice > mash > pellets.

6. The plucked empty bodies of birds given the free choice had a significantly lower concentration of crude protein than the plucked empty bodies of birds fed on mash or pellets. There were no significant differences between the treatments in the plucked empty body concentrations of fat, calcium or phosphorus.

7. Financial analysis indicated that free‐choice feeding was more profitable than feeding mash or pelleted diets to broiler chickens by 3.6% and 33.4% respectively.     

Assessment of the nutritional status of indigenous scavenging chickens in Ada’a district,Ethiopia

The nutritive values of scavenging feed resource bases and effects of season and chickens age on the latter were studied in smallholder farms in Aad’a, Ethiopia. The study included 210 households and 208 chickens. The mean weight of crop contents in all age groups ranged from 26.2 to 28.2 g, while it was 29.8 g and 24.7 g in the harvesting and non-harvesting seasons, respectively. Grains represented 48–49% of the mean weight of crop contents in all age groups and it was significantly higher (P < 0.05) during the harvesting season than the non-harvesting period in both age groups. Kitchen wastes were next in abundance (26–29%) and were significantly more abundant in non-harvesting season in growers only and in hens than in growers (P < 0.001). The dry matter, crude protein, crude fiber, calcium, phosphorous and metabolizable energy levels of crop contents were 91.1–92.5%, 12.9–15.5%, 4.17–7.07%, 0.43–0.90%, 0.28–0.38% and 3404.3–3636.2 Kcal, respectively. Crude protein, calcium and phosphorus levels were below the requirements for egg production and growth. The scavenging feed resource base was critically deficient in these nutrients during the harvesting season. As these nutrients are vital for production, supplementary feeds rich in these nutrients would probably result in increased egg production and optimum growth.     

Prevalence and incidence of Newcastle disease and prevalence of Avian Influenza infection of scavenging village chickens in Timor-Lesté

A longitudinal study to monitor prevalence and incidence of antibodies against Newcastle disease (ND) virus and prevalence of antibodies against Avian Influenza (AI) virus in scavenging village chickens was conducted in 20 villages within 4 districts of Timor-Lesté. A total of 3600 blood samples was collected from 1674 individual birds in 300 household chicken flocks during three sampling periods (December 2008-February 2009, March-May 2009, and June-August 2009). The mean interval between household visits was 101.6±1.9 days. None of the birds enrolled in the study was vaccinated against ND or AI. A haemagglutination inhibition (HI) test was used to determine antibody titres against ND virus and a competitive ELISA and HI tests were used to detect antibody against AI virus. The bird-level ND seroprevalence pooled across all samplings (adjusted for clustering by households) was 4.4% (95% CI 3.5-5.2). The bird-level ND seroprevalence in each of the three sampling periods (adjusted for clustering by household) was 3.0% (95% CI 2.0-4.0), 6.6% (95% CI 5.1-8.0) and 3.6 (95% CI 2.5-4.6), respectively. A total of 12.6% individual birds tested ND seropositive at least once over the total study period (95% CI 10.5-14.7). The flock-level ND seroprevalence (at least one bird tested had antibodies against ND virus) pooled across all samplings was 15.9% (95% CI 13.5-18.3). A total of 35.3% flocks had a minimum of one bird being ND seropositive at least once over the study period. The bird-level incidence rate for the period between the first and the second sampling and between the second and the third sampling was 5.6 (95% CI 4.1-7.5) and 0.5 (95% CI 0.5-3.8) per 10,000 bird-years-at-risk, respectively. A total of 1134 serum samples from the last sampling period between June and August 2009 was tested for antibodies against AI virus. Only 4 samples tested Influenza A positive, indicating a bird-level seroprevalence level for Influenza A of 0.4% (CI 0.0-0.7%). These Influenza A positive samples were further tested for HI antibodies against AI virus subtypes of H5N1, H5N3, H7N3 and H9N2, but all tested negative, suggesting that the influenza antibodies in those four birds resulted from exposure to low pathogenic AI viruses of different H subtypes. Our results indicate that village chickens in Timor-Lesté are exposed to ND virus; there was a higher risk of infection during the early months of 2009 than either immediately prior or subsequent to this. No evidence of infection of village chickens with H5, H7 or H9 AI viruses was detected in this study.     

Determinants of farmers’ choice for veterinary service providers in Nepal Mountains

This study was aimed to analyze factors that affect farmers' choice for major actors of veterinary service providers in village areas of Kaski district of Nepal, with the objective of identifying choice-specific attributes which could be addressed for improving the penetration of professional veterinary services in village areas. The information was obtained from 125 farmers using a structured questionnaire. A proportional hazard model was used for data analysis because of its ability to accommodate the attributes of both the chooser and the choice simultaneously. The results showed that village animal health workers (VAHWs) were the most preferred service providers followed by veterinarians and mid-level technicians. The farmers' age and education level had a significant but inverse relationship with the probability of choosing any of the three service providers. From our study, we found that the main choice-specific attributes with a significant impact on the choice probability was the distance to the preferred service provider. Since there was a high preference for VAHW, this suggests the possibility of poorly trained service providers dominating veterinary service market in village areas of Kaski district. Hence, the efforts put forward in the legalization of VAHW system in Nepal should first address the constraints that obstruct the accessibility of professional veterinary service providers in village areas.     

Understanding stakeholders’ opinions and preferences for non-native pet trade management in Florida

There is growing recognition of the link between the non-native pet trade and the introduction and establishment of invasive species due to the release and escape of non-native pets. However, it is unclear whether participants in the pet trade recognize the magnitude of this invasion risk. Successful mitigation of the pet trade invasion risk requires stakeholder support for, and participation in, regulations. We conducted 29 interviews in Florida to investigate key stakeholders’ opinions about the pet trade invasion risk and the effectiveness of potential regulations to mitigate this risk. Respondents framed the effectiveness of regulations in terms of their feasibility. Respondents also identified lack of trust and the existence of an adversarial relationship among stakeholder groups as major barriers to managing the pet trade invasion risk. Compliance with regulations may be improved if policy makers and managers utilize participatory decision-making to engage stakeholders in management of this risk.     

Effectiveness of dietary 25‐ and 1‐hydroxycholecalciferol in combating tibial dyschondroplasia in broiler chickens

1. Three experiments were carried out to determine the effects of feeding diets containing different concentrations of cholecalciferol, 1 a‐hydroxychole‐calciferol (1‐HCC), 25‐hydroxycholecalciferol (25‐HCC), 1,25‐dihydroxycholecalcif‐erol (1,25‐DHCC) and ascorbic acid on the incidences and severities of tibial dyschondroplasia (TD) at 3 weeks of age in male broiler chicks.

2. In experiment 1, replacing 75 μg cholecalciferol/kg with the same weight of 25‐HCC decreased significantly (P<0.01) the incidence of TD from 65 to 10%.

3. In experiment 2, the incidence of TD in the control group was lower, but feeding amounts of 25‐HCC up to 250 μg/kg had a linear effect on the incidence of TD that was significant at P=0.06. There was no effect or interactions with dietary addition of 250 mg ascorbic acid/kg. Dietary addition of 5 μg 1‐HCC/kg decreased TD incidence from 21 to 5%, though the effect was not significant (P>0.1).

4. TD incidence in experiment 3 was too low to determine an effect of 25‐HCC or 1,25‐DHCC on TD incidence, though in this, as in both other experiments, the severities of TD lesions were always lower with diets containing cholecalciferol metabolites.

5. Hypercalcaemia was not observed after feeding up to 250 μg 25‐HCC/kg in either experiments 2 or 3.

6. It is concluded that 25‐HCC may be an effective practical means of improving broiler leg health by alleviating the incidence and severity of TD.     

Husbandry and trade of indigenous chickens in Myanmar—Results of a participatory rural appraisal in the Yangon and the Mandalay divisions

There is a variety of professions working with village chickens in developing countries, including farmers, veterinarians and chicken traders. People from all these occupations were involved in a participatory rural appraisal to investigate husbandry practices and trade of village chickens in Myanmar. Data were collected in two climatically different regions of the country, in the Yangon and in the Mandalay divisions. The breeding and training of fighting cocks was practised only in the Mandalay division, with well-trained birds sold for very high prices. Apart from this, chickens were raised in both regions mainly for small disposable income and were generally sold when money was needed, in particular during religious festivals. Chicken traders on bicycles, often called ‘middle men’, usually purchase birds from farmers in about 10 villages per day. Several ‘middle men’ supply birds to wealthier chicken merchants, who sell these birds at larger chicken markets. There is in general limited knowledge among farmers about the prevention of Newcastle disease via vaccination. Commercial indigenous chicken production is practised in Myanmar, but family poultry farming dominates indigenous chicken production in the country.     

Effects of interferon-γ knockdown on vaccine-induced immunity against Marek’s disease in chickens

Interferon (IFN)-γ has been shown to be associated with immunity to Marek’s disease virus (MDV). The overall objective of this study was to investigate the causal relationship between IFN-γ and vaccine-conferred immunity against MDV in chickens. To this end, 3 small interfering RNAs (siRNAs) targeting chicken IFN-γ, which had previously been shown to reduce IFN-γ expression in vitro, and a control siRNA were selected to generate recombinant avian adeno-associated virus (rAAAV) expressing short-hairpin small interfering RNAs (shRNAs). An MDV challenge trial was then conducted: chickens were vaccinated with herpesvirus of turkey (HVT), administered the rAAAV expressing shRNA, and then challenged with MDV. Tumors were observed in 4 out of 10 birds that were vaccinated with HVT and challenged but did not receive any rAAAV, 5 out of 9 birds that were administered the rAAAV containing IFN-γ shRNA, and 2 out of 10 birds that were administered a control enhanced green fluorescent protein siRNA. There was no significant difference in MDV genome load in the feather follicle epithelium of the birds that were cotreated with the vaccine and the rAAAV compared with the vaccinated MDV-infected birds. These results suggest that AAAV-based vectors can be used for the delivery of shRNA into chicken cells. However, administration of the rAAAV expressing shRNA targeting chicken IFN-γ did not seem to fully abrogate vaccine-induced protection.     

Farmers’ perceptions of the causes of low reproductive performance in cows kept under low-input communal production systems in South Africa

The objective of the study was to determine farmers’ perceptions of the causes of low reproductive performance in Nguni cows raised on communal rangelands in the Eastern Cape Province of South Africa. Data were collected using participatory rural appraisals and structured questionnaires that were administered to 551 randomly selected farmers from ten communities in the Eastern Cape. Cattle herd sizes ranged from 3 to 11 and were mainly composed of cows. Cattle sales were ranked as the most important use of cattle in all the villages. Tick-borne diseases and poor animal condition were reported as chief constraints of cattle production in most communities. More than 60% of the interviewees reported that the age at puberty and age at first calving for their cows varied between 18 and 36, and 24 and 48 months, respectively. About 95% of the respondents reported long calving intervals and low bull numbers as major causes of low reproductive performance in cows in the communal areas. It was concluded that farmers perceived delayed age at puberty and at first calving, long calving intervals and low bull numbers as the major causes of low reproductive performance in Nguni cows raised on communal rangelands in South Africa.     

Seroprevalence of infectious laryngotracheitis disease in backyard chickens in villages of Ada’a district,Oromia, Ethiopia: first report

Tropical Animal Health and Production - As per the report from the OIE in 2005, infectious laryngotracheitis (ILT) has not been yet reported in Ethiopia. Hence, considering the evident clinical...     

Alteration of fatty acid profile and nucleotide-related substances in post-mortem breast meat of α-lipoic acid-fed broiler chickens

1. The present study was conducted to determine the effects of α-lipoic acid supplementation on post-mortem changes in the fatty acid profile and concentrations of nucleotide-related substances, especially those of a taste-active compound, inosine 5?-monophosphate, in chicken meat.

2. Mixed-sex broiler chicks aged 14 d were divided into three groups of 16 birds each and were fed on diets supplemented with α-lipoic acid at levels of 0, 100 or 200 mg/kg for 4 weeks. Blood and breast muscle samples were taken at 42 d of age under the fed condition and then after fasting for 18 h. The breast muscle obtained from fasted chickens was subsequently refrigerated at 2°C for one and 3 d.

3. α-Lipoic acid supplementation did not affect any plasma metabolite concentration independently of feeding condition, while a slight increase in plasma glucose concentration was shown with both administration levels of α-lipoic acid. In early post-mortem breast muscle under the fed condition, α-lipoic acid had no effect on concentrations of fatty acids or nucleotides of ATP, ADP, and AMP.

4. In post-mortem breast tissues obtained from fasted chickens, total fatty acid concentrations were markedly increased by α-lipoic acid feeding at 200 mg/kg irrespective of length of refrigeration. This effect was dependent on stearic acid, oleic acid, linoleic acid and linolenic acid. However, among fatty acids, the only predominantly increased unsaturated fatty acid was oleic acid.

5. Dietary supplementation with α-lipoic acid at 200 mg/kg increased the inosine 5?-monophosphate concentration in breast meat and, in contrast, reduced the subsequent catabolites, inosine and xanthine, regardless of the length of refrigeration.

6. Therefore, the present study suggests that α-lipoic acid administration altered the fatty acid profile and improved meat quality by increasing taste-active substances in the post-mortem meat obtained from fasted chickens.