狮子犬睾丸支持细胞瘤手术

该犬为白色杂交狮子犬，5岁，体重7kg，主诉：该犬来院就诊前1年发现右侧睾丸肿大，畜主起初以为是炎症，口服青霉素，未见好转。后来又有乱爬跨现象，呈雌性犬性行为，乳房异常发育，易脱毛，排尿动作异常，且一侧睾丸逐渐增大，近几天食量减少，故来院就诊。检查：该犬右侧睾丸肿大，已有拳头大小，且阴囊局部皮肤着色深，触诊质地硬实，左侧睾丸未见异常，体温38．5℃。     

京巴犬睾丸支持细胞瘤病理学观察病例

犬的生殖系统疾病中常见的肿瘤主要有3种,即支持细胞瘤(supporting cell tumor)、精母细胞瘤和间质细胞瘤.犬睾丸支持细胞瘤又称赛特利细胞瘤、赛托利细胞瘤(sertoli cell tumor)或足细胞瘤[1],是这3种肿瘤中最易辨认的肿瘤.     

一例京巴犬睾丸支持细胞瘤的病理学观察

犬睾丸支持细胞瘤又称赛特利细胞瘤、赛托利氏细胞瘤（sertoli cell tumor）或足细胞瘤,该肿瘤体积大,易发生于异位睾丸,发病后肿瘤睾丸分泌雌激素,引起雌性化、脱毛及血恶液质（blood dyscrasia）等雄性动物雌性化综合征。2007年3月份,笔者曾接诊了1只患睾丸肿瘤的宠物犬,采取手术治疗,取手术剥离和切除的左、右侧睾丸进行了病理检查,现将结果报道如下。     

犬多发性肾囊肿1例

2001年10月21日，长城须崎公司保卫科牵来1只雄性狼犬就诊。主诉：该犬6岁，体重约30kg，从警犬基地引进已有一年，刚购回时配种1次，母犬怀孕并产仔7只，后多次配种均未成功。近半年来虽显消瘦，但食欲尚可，近1月来消瘦明显．3d前即食少，之后不食，并表现呼吸急促。     

犬睾丸肿瘤的治疗

犬睾丸肿瘤是犬生殖系统疾病中较为棘手的疾患，现已被认为是中老年犬的一种常见疾病。我院于2004年4月20日接诊1例睾丸支持细胞瘤患犬，现报告如下。     

犬睾丸精原细胞癌一例的诊疗

介绍了1例老龄犬睾丸精原细胞癌的诊断和手术治疗.通过临床症状检查、B超检查、电解质检测、血常规检查、生化检查和肿瘤组织病理检查,确诊为睾丸精原细胞癌,运用手术方法摘除瘤体后,3个月后回访该犬正常生存,饮食正常,体重增加.     

一例犬唾液腺-颌下腺黏液囊肿的诊治

11岁雄性贵宾犬,颈部腹侧肿胀一个月.经临床检查、实验室检查、X光和穿刺检查,确诊为唾液腺-颌下腺黏液性囊肿,采取手术摘除疗法.     

犬丁胺卡那引起肌无力诊治一例

丁胺卡那为氨基糖甙类抗菌素,兽医临床应用较为普遍.笔者曾遇一例丁胺卡那引起犬重症肌无力病例,经治疗康复,现报告如下. 1 病例 本地家犬,公,4月龄,6kg,因腹泻就诊,曾用丁胺卡那(0.1g/ml)2.0ml×2肌注,每天1次,连用3d.结果第2d用后0.5h即发现该犬瘫软在地,15min内就诊本院.检查体温37.3℃,肌无力,心音微弱,呼吸浅.诊断为丁胺卡那中毒,随用硫酸新斯的明0.3ml肌注;葡萄糖酸钙10.0ml、50%葡萄糖20.0ml、地塞米松1.0ml、5%葡萄糖200.0ml,静注,吸氧.在静注1/3后,即有明显好转.30min后再肌注硫酸新斯的明0.3ml,即愈.     

犬多发性肥大细胞瘤病理学诊断与分析

犬肥大细胞瘤(Canine mast cell tumor, MCT)是起源于真皮组织肥大细胞的恶性肿瘤,最常见于躯干、四肢和头颈部。犬肥大细胞瘤的临床表现多样,其表面可能脱毛、溃疡、红斑、色素过度沉着。一例患犬通过临床症状检查、血常规、血液生化、C-反应蛋白(C-reactive protein, CRP)、X射线以及病理组织学观察,最终确诊为犬的多发性肥大细胞瘤。对该犬实施了肿瘤及周围健康组织切除手术,并建议术后进行化疗,为后续提高犬多发性肥大细胞瘤治疗和诊断水平提供参考。     

F-2毒素致体外培养大鼠睾丸支持细胞DNA损伤

为探讨F-2毒素对雄性生殖机能的影响,取大鼠睾丸支持细胞进行体外培养,运用单细胞凝胶电泳技术检测51、0、20和40 mg/L的F-2毒素攻毒后24 h支持细胞DNA的损伤情况。结果显示,F-2毒素在一定浓度范围内对体外培养的支持细胞DNA产生损伤作用,且受损程度随着F-2毒素攻毒剂量而升高,具有明显量效关系。除5 mg/L剂量组外,其余各组的细胞受损率、彗星尾长和DNA损伤程度与阴性对照组相比差异极显著（P〈0.01）,表明F-2毒素对体外培养的大鼠睾丸支持细胞有毒性作用,能够损伤细胞DNA。     

Spermatocytic seminoma with neuroectodermal differentiation and sertoli cell tumor in a dog

Two distinct nodules developed in a cryptorchid testis of an 8-year-old male West Highland White Terrier. One nodule was a Sertoli cell tumor. The other was a spermatocytic seminoma with focal primitive neuroectodermal differentiation: formation of Homer-Wright rosettes and perivascular pseudorosettes, with immunoreactivity for S-100 protein, neuron-specific enolase, synaptophysin, neurofilament-68 kDa, microtubule-associated protein 2, and vimentin. The dog was alive and healthy 2 years after castration.     

A case of multiple unilateral corneal epithelial inclusion cysts in a dog

A three-year-old neutered female Labrador cross was presented for investigation of two corneal epithelial inclusion cysts affecting the left eye. The aetiopathogenesis of the cyst formation was suspected to be traumatic. The cysts were removed successfully by superficial keratectomy, followed by a third eyelid flap. Histologically, both lesions were represented by cystic formations lined with multi-layered squamous epithelium, consistent with stromal inclusion of surface corneal epithelium. Complete recovery was achieved, and there was no recurrence at six month follow-up.     

Corneal squamous cell carcinoma in a dog: a case report

Purpose:  To report a case of primary corneal squamous cell carcinoma (SCC) in an English Bulldog. In addition, immunohistochemistry of the corneal tissue mass was performed using a panel of antibodies. A prominent feature of the present case was the clinical history of chronic keratitis due to eyelid abnormalities.
Results:  No papillomavirus antigen was detected in section of normal or neoplastic corneal tissue. The corneal epithelial cells were positive for pancytokeratins AE1/AE3 and MNF116, and E-cadherin. The neoplastic cells in close proximity to the normal epithelial lining were positive for both pancytokeratins and E-cadherin with gradual loss of staining toward the center of the neoplastic mass. Rare neoplastic cells demonstrated positive staining for caspase 3 and a large number was strongly positive for GADD45 and p53.
Conclusion and discussion:  The observed loss of the various cytokeratins, the strong p53 expression, and low numbers of caspase 3 positive cells were suggestive that a p53 mutation may have caused this primary corneal SCC. Over-expression of the tumor-suppressor gene p53 is likely to be a consequence of ultraviolet radiation exposure. Two factors, however, may have played a role in the formation of this primary corneal SCC: chronic irritation of the corneal surface (microtrauma) and exposure to UV radiation.     

Computed tomographic diagnosis of unilateral cavernous sinus syndrome caused by a chondrosarcoma in a dog: a case report

An eight-year old Rottweiler dog was presented with signs of enophthalmia, ptosis, anisocoria and mydriasis of the right eye, which showed visual disturbance, reduced or absent reflexes, and ophthalmoplegia. Consensual pupillary light reflex was also absent in the left eye. These neurological deficits were compatible with cavernous sinus syndrome. Computed tomography images of the cavernous sinus and the optical fissure revealed a mildly calcified mass arising from the right presphenoid bone extending further caudally into the orbital foramina. This extension of the mass affected the normal function of several cranial nerves. The dog was euthanased within one year of the initial presentation following development of forebrain signs. A chondrosarcoma was diagnosed histologically after necropsy.     

Mast cell sarcoma of the larynx in a dog: a case report

An 8-year-old, spayed, female miniature poodle was presented to the University of Pennsylvania Veterinary Hospital (UPVH) with signs of mild respiratory distress related to increasing laryngeal obstruction. The dog was found to have a unicentric mast cell sarcoma involving the larynx, with tumour infiltration of the tissues between the thyroid and arytenoid cartilages as well as subglottic narrowing. Treatment and differential diagnosis are described.     

Symptomatic granular cell tumor involving the pituitary gland in a dog: a case report and review of the literature

A granular cell tumor involving the pituitary gland, optic chiasm and ventral pyriform lobes was discovered in a 12-year-old Labrador Retriever. Clinical signs included acute blindness, seizures, ataxia, weakness, and behavioral changes. The diagnosis was established by histopathologic and ultrastructural examination of neoplastic tissues collected at necropsy. Granular cell tumors involving the central nervous system are well documented in humans but rarely have been described in dogs. The location of the neoplasm and the clinical symptoms seen in this dog closely parallel those of a rare syndrome in humans commonly described as symptomatic parasellar or pituitary granular cell tumors. The cell of origin for these tumors is still highly debated, and attempts to characterize human granular cell tumors through immunohistochemistry have produced conflicting results. An immunohistochemical profile of this neoplasm revealed focal positive staining for vimentin with a lack of staining for neuron-specific enolase, glial fibrillary acidic protein, S-100, and synaptophysin. All neoplastic cells were strongly positive with the periodic acid-Schiff reaction.     

Intracranial granular cell tumor in a dog

A 12-year-old female miniature poodle showed a 3-month history of neurological signs. Magnetic resonance imaging disclosed a high intensity tumor mass in the right cerebral hemisphere with compression of the lateral ventricle. At necropsy, a 2 x 3 cm white, friable mass was found in the right ventral pyriform lobe. Microscopically, the tumor cells were large, polygonal to round cells supported by a sparse fibrovascular stroma. The tumor cells typically possessed finely granular, pale eosinophilic cytoplasm with strongly positive periodic acid-Schiff (PAS) reaction. The tumor cells were immunopositive for vimentin, NSE and S-100. Ultrastructurally, the tumor cells showed large amounts of granules in the cytoplasm, and absence of basement membrane. Based on the above-mentioned findings, the intracranial granular cell tumor was diagnosed.     

Malignant nerve-sheath tumor with divergent and glandular differentiation in a dog: a case report

Malignant nerve-sheath tumor with divergent differentiation including epithelial components was diagnosed in an 8-year-old Labrador retriever. The myelographic, morphologic, and immunohistochemical findings confirmed the diagnosis. The tumor was located in the peripheral nerve roots at the first and second lumbar vertebrae. The dog survived for 161 days after resection of the tumor.