Hepatoprotective activity of Calotropis procera flowers against paracetamol-induced hepatic injury in rats

Hydro-ethanolic extract (70%) of Calotropis procera flowers was prepared and tested for its hepatoprotective effect against paracetamol-induced hepatitis in rats. Alteration in the levels of biochemical markers of hepatic damage like SGPT, SGOT, ALP, bilirubin, cholesterol, HDL and tissue GSH were tested in both treated and untreated groups. Paracetamol (2 g/kg) has enhanced the SGPT, SGOT, ALP, bilirubin and cholesterol levels and reduced the serum levels of HDL and tissue level of GSH. Treatment with hydro-ethanolic extract of C. procera flowers (200 mg/kg and 400 mg/kg) has brought back the altered levels of biochemical markers to the near normal levels in the dose dependent manner.     

In-vitro schizonticidal screening of Calotropis procera.

Following an ethnobotanical approach, the ethanol extracts of Calotropis procera leaves, stems, roots, flowers and buds have been screened in vitro for antimalarial activity against chloroquine (CQ)-sensitive and CQ-resistant Plasmodium falciparum strains.     

In vitro spasmolytic effect of aqueous extract of Calotropis procera on Guinea-pig trachea smooth muscle chain

The aqueous extract of Calotropis procera was evaluated for its spasmolytic effect using in vitro trachea smooth muscle chain of Guinea-pig. The extract (50, 100 and 200 microg/ml) showed a dose-dependent relaxant activity probably exhibited through the direct relaxant action on the smooth muscle.     

棉花环柄菇的驯化试验初报

通过对棉花环柄菇子实体组织分离方法及培养基筛选试验，得到了优良母种，并选出最适的母种培养基。为棉花环柄菇的进一步驯化奠定了基础。     

Analgesic activity of Calotropis gigantea flower

The alcoholic extract of the flowers of Calotropis gigantea was administered orally and explored for its analgesic activity in chemical and thermal models in mice. In acetic acid induced writhing test, an inhibition of 20.97% and 43.0% in the number of writhes was observed at the doses of 250 and 500 mg/kg, respectively. In the hot plate method the paw licking time was delayed. The analgesic effect was observed after 30 min of dose administration which reached its maximum after 90 min.     

Puerarin exhibits weak estrogenic activity in female rats

A weak estrogenicity of puerarin on reproductive organs was addressed in female rats. In short-term treatment, immature ovariectomized rats were injected with 0.7 mg/kg BW/day of puerarin, for 14 days. Puerarin did not increase uterus weights, endometrium and myometrium areas, and the percent of cornified cells (%Co), but it increased the number of uterine glands. In long-term treatment, mature rats were injected with 7.0 mg/kg BW/day of puerarin for 140 days. Puerarin did not increase uterus weights, endometrium and myometrium areas, and the number of uterine glands, but a significant increase in the %Co was observed from day 98 onwards.     

Latex proteins from the plant Calotropis procera are partially digested upon in vitro enzymatic action and are not immunologically detected in fecal material

Soluble proteins from the latex of Calotropis procera (LP) were investigated in vitro and in vivo for digestibility as the latex has previously been shown to produce considerable toxic effects on animals. The latex is also an important biologically active compound that displays antiinflammatory and antidiarrhea properties. The proteins were digested by the action of trypsin, pepsin or chemotrypsin as revealed by gel filtration and SDS-PAGE analysis. Furthermore, the full LP digestion was easily achieved by protease treatment. Rabbit polyclonal antibodies raised against LP failed to detect cross-reactive molecules in fecal material of experimental rats following 35 consecutive days of LP consumption in water. Similar patterns of electrophoresis were observed for the negligible amounts of protein observed in the fecal extracts of control and test animals. No death or toxic effects were observed among animals. Taken together these results suggest that harmful and toxic effects on animals of the latex from C. procera are present in its rubber and low molecular weight fractions rather than its protein content.     

西双版纳热区木霉的分离及抑菌活性初步研究

对采自西双版纳热区枯枝落叶上的木霉进行分离,共获得19株木霉(Trichoderma spp.)。抑菌活性测定结果表明,19株木霉的发酵液对3种病原真菌(Sclerotinia sclerotiorum,Botrytiscinerea Pers.,Colletotrichum sp.)均具有不同程度的抑制作用,其中菌株SWFC1291对3种病原真菌的抑菌率均超过了80%;SWFC1511对Botrytiscinerea Pers.的抑菌率达90%。19株木霉中,SWFC2470对红酵母(Rhodotorulasp.)的抑制效果较好,抑菌圈直径达到了2.65cm;SWFC2397,SWFC2470,SWFC3499和SWFC3568这4株菌对大肠杆菌(Escherichia coli)的抑制作用明显,其中SWFC3568的抑菌圈达到了1.60 cm。SWFC1925和SWFC2470两个菌株对红酵母和大肠杆菌均具有抑菌活性,其中SWFC2470菌株对两种菌的抑制作用较强。     

Preliminary study on antihyperglycemic and antihyperlipaemic effects of Butea monosperma in NIDDM rats

Oral administration of the ethanolic extract of the Butea monosperma seeds (300mg/kg b.w.) exhibited significant antidiabetic, hypolipaemic and antiperoxidative effects in non-insulin dependent diabetes mellitus rats.     

Leishmanicidal activity of Maytenus illicifolia roots

In vitro evaluation of leishmanicidal activity of aqueous and ethanolic extracts of Maytenus illicifolia against three species of Leishmania. The root extracts showed differential activity against parasites, whereas the aerial part of the extracts was inactive.     

Antibacterial activity of Borreria verticillata roots

Borreria verticillata roots methanolic extract exhibited a broad antibacterial activity against multiresistant strains of Pseudomonas aeruginosa.     

树冠修剪和切根处理对红松母树雌雄球花分化的影响

以红松种子园中的生长形状相对一致的嫁接母树为试验材料,研究不同树冠修剪和切根方法对母树雌雄球花分化数量的影响。结果表明,不同处理均对母树雌雄球花分化数量有显著的促进作用,其中:剪去树冠第一层轮枝和顶梢部分,在第二层轮枝中选留3个生长健壮且分布均匀的侧枝,其余侧枝全部剪除的处理方法,对雌球花分化数量的增加作用最为明显,差异达到极显著水平;切断试验母树3/4环形沟中的根系和试验母树环形沟中全部根系的处理方法,对雌雄球花分化数量的增加作用最为明显,差异达到极显著水平。     

Preliminary toxicity study on the individual and combined effects of Citrullus colocynthis and Nerium oleander in rats

The toxicity of diet containing 10% of Citrullus colocynthis fruits or 10% of Nerium oleander leaves or their 1:1 mixture (5%+5%) for rats treated for 6 weeks was determined. Dullness, ruffled hair, decreased body weight gains and feed efficiency, and enterohepatonephropathy characterised treatment with C. colocynthis and N. oleander given alone. Diarrhoea was a prominent sign of C. colocynthis poisoning. Organ lesions were accompanied by leucopenia, anaemia and alterations in serum AST, ALT and ALP activities and concentrations of total protein, albumin, urea, bilirubin and other serum constituents. Feeding the mixture of C. colocynthis and N. oleander caused more marked effects and death of rats.     

牛角瓜3个居群的结实特性研究

调查分析东川、元江和元阳3个干热河谷居群的牛角瓜结实等特征。结果表明,1元阳居群的牛角瓜单株结实量、单位冠幅结实量和单枝结实量最高,分别为102.4个/株、35.1个/m2和34.6个/枝,且3个居群间这3个指标差异极显著;2居群内单株、单位冠幅和单枝结实量3个指标差异极显著;3各居群的单株结实量与单枝结实量、单位冠幅结实量和分枝相关性状间呈线性相关。元阳是牛角瓜人工栽培的首选区域,通过对结实量大的优良单株进行选育,可极大地提高其果实产量。     

Antinociceptive activity of Euphorbia heterophylla roots.

Following an identified use of the plant as analgesic in traditional medicine, the hexane, chloroform and ethyl acetate extracts of Euphorbia heterophylla root have been tested for antinociceptive activity in rats. All extracts showed significant effects at doses of 150-300 mg/kg i.p.     

Antibacterial activity of Jatropha multifida roots.

Hexane, ethyl acetate, chloroform and methanol successive extracts of Jatropha multifida yellow rootbark, red rootbark and rootwood effectively inhibited the growth of B. subtilis and S. aureus at concentration of 200 microg/disk.     

五加肖个木虱研究初报

五加肖个木虱 Triozidusacanthopanaicis L i是危害短梗五加 Acanthopanax brachy-pus Miq.的专食性害虫。该虫在辽宁地区 1年发生 2代 ,以成虫越冬。 4月下旬开始补充营养 ,5月上旬交配产卵。若虫共 6龄 ,危害期每代持续 5 0～ 70天 ,用 40 %氧化乐果乳油80 0～ 1 0 0 0倍防治 ,效果最佳。     

栎线小卷蛾初步研究

栎线小卷蛾危害梨树花和嫩叶，系国内新记录种。在临夏１年发生２ 代，以卵在枝条上越冬。经室内药效测定和田间防治试验结果表明，采用８０％ 敌敌畏１ ０００ 倍、或２５％ 溴氰菊酯２ ０００ 倍、２５％ 三氟氯氰菊酯３ ０００～４ ０００倍、３０％ 赛丹３ ０００倍，防效显著。     

红松组织培养技术的初步研究

以ＭＳ为基本培养基,加入不同种类和浓度的激素,对红松离体胚和２￣３ａ生幼苗的顶芽进行组织培养。在含有激素的培养基上,离体胚在量愈伤组织和畸形苗。顶经过４－５个月培养后,在细胞分裂素含量较高的培养基上形成少量侧芽,在生长素类激素含量较高的发生不定根。     

光肩星天牛受辐雌成虫的不育性初步研究

采用^60CoT射线辐光肩星天年Anoplophora glabripennis（Motschulsky）雌成虫，研究其不育性。结果表明，辐照对其取食行为无影响；对其产卵行为产生了显著影响，其产卵量随着辐照剂理的提高而明显降低；对其寿命和交尾情况的影响主要表现在200Gy辐照上，致使寿命大幅度降低且见交尾现象，因而受辐雌成虫也具有一定的不育性。