用于细管冻精生产的陶赛特种公羊选定程序

探讨了用于细管冻精生产陶赛特种公羊的选定程序,使陶赛特羊的细管冻精质量在源头上得到了保证。     

不同品种绵羊的细管冻精解冻质量检测分析

选取8个不同品种羊(引进品种2个,本地品种4个,培育品种2个)的细管冻精,进行外观、剂量、精子活力、前向运动精子数和精子顶体完整率指标的检测分析。结果显示,各细管冻精外观均完好,符合生产标准;剂量检测值为(0.21±0.01) mL,均符合国家标准(≥0.18 mL);精子活力为(37.50%±1.70%)～(51.0%±3.70%),其中,陶赛特羊精子活力值最高,黑裘皮藏羊精子活力值最低;前进运动精子数为≥3×10⁷个,符合国标;精子顶体完整率为(71.90%±1.13%)～(81.23%±1.0%),其中,西藏(草地型)羊精子顶体完整率最高,陶赛特羊精子顶体完整率最低。8个品种的羊冻精在解冻复苏后各项指标均达到配种生产要求。引进品种的精子活力比本地品种高,前进运动精子数和顶体完整率等其他指标有高有低。     

高海拔地区陶赛特肉羊细管冻精技术与应用推广

细管冻精技术是20世纪60年代法国研究开发出的一项新型人工授精技术.青海省家畜改良中心2007年8月成功研制开发陶赛特良种肉样细管冻精,检测冻后活力明显优于省外水平,填补了我省自行研制生产肉羊冻精技术的-项空白.陶赛特良种肉样细管冻精研制成功,为缓解种羊紧缺矛盾,减少投资浪费,促进我省肉羊产业的发展,提高农牧民群众经济收入具有十分重要的现实意义,最大限度地发挥了肉用种羊优良性状达到使畜种资源得到充分合理利用,使我省肉羊生产水平全面快速发展.     

多浪羊细管冷冻精液制作技术的研究

为保存和利用优秀地方品种多浪羊的遗传资源,试验在多浪羊原产地进行了细管冻精制作试验.用采集的多浪羊种公羊精液,比较5种冻精保存液配方的细管冻精冷冻效果,测定解冻后精子活力.结果表明:4.83%乳糖保存液解冻后精液的活率平均为36% ,优于其它4种保存液,且差异极显著(P<0.01),解冻后精子的畸形率也均低于其它4种稀释液,差异极显著(P<0.01).表明4.83%的乳糖保存液配方是理想的绵羊细管冻精稀释液.     

无角陶赛特种公羊细管型冻精冷配小尾寒羊受胎效果的研究

内蒙古乌兰察布市为了改善小尾寒羊的肉质，提高其出肉率，该市大力开展陶寒杂交改良，打造乌兰察布市绿色肥羔生产供应基地。用无角陶赛特细管冷冻精液冷配小尾寒羊既能解决无角陶赛特种公羊的紧缺，又能最大限度地提高陶赛特种公羊的利用率和扩大配种辐射区域。2003年，笔者在乌兰察布市3个小尾寒羊相对集中的旗县开始冷配试验。     

波尔山羊细管冻精技术及应用

细管冻精技术是20世纪60年代法国研究开发出的一项新型人工授精技术.羊细管冻精在我国1999年才开始研目生产,目前仅有几家能生产且使用普及率很低.我站现有成套法国凯苏公司细管冻精生产设备,1999年初批量生产波尔山羊细管冷冻精液,检测冻后活力明显优于颗粒冻精.经人工授精后统计,羊细管冻精受胎率比颗粒冻精高5～15个百分点.目前我站已向省内外推广应用.     

公牛塑料细管冻精制作方法与应用效果的研究

本试验就细管冻精和颗粒冻精的冷冻效果和受胎情况进行了对比分析。结果表明，采用设定的工艺流程制作的细管冻精与颗粒冻精，其精子的解冻活率和复苏率没有显著差异（ｐ〉０．０５），采用水平冻结方式和垂直冻结方式生产的细管冻精，其精子的解冻活率和复苏率也无显著差异（ｐ〉０．０５）。但细管冻精的情期受胎率和一次输精受胎率却显著高于颗粒冻精。表明细管冻精的应用效果优于颗粒冻精。     

肉牛冻精配种关键点

(一)塑料细管冻精的质量与贮存 细管冻精质量是决定受胎率高低的首要问题.首先要选好生产塑料细管冻精单位、养好种公牛、鲜精不好的不冷冻、冻后不符合标准的不贮存.存选购细管冻精方面,要查询细管冻精来源,并对冻精抽样显微镜检查,要求精子活力0.3以上.不合格的不用.     

提高黄牛冻配受胎率的关键技术

细管冻精质量的好坏，是决定受胎率高低的首要问题。因此，首先是生产塑料细管冻精的单位要选好、养好种公牛，坚持鲜精不好的不冷冻，冻后不合符标准的不贮存。在选购细管冻精方面，强调询查细管冻精来源的情况，并对冻精抽样显微镜检查，不合格的坚决不用。要求精子活力在0．3以上。在给母牛输精时要认真把握好细管冻精质量关。     

细管冻精与安瓿冻精的受胎效果对比

细管冻精是目前最为优良的一种精液冷冻方法。本试验的目的是检验细管冻精的输配效果,为推广应用奶牛细管冻精提供科学依据。一、材料1.细管冻精、安瓿冻精(均为瑞安市畜种站生产,批号不详)。冻精活力(下限)     

癸氧喹酯干混悬剂含量测定的改进

采用高效液相色谱法测定癸氧喹酯干混悬剂的含量,在2-250μg/mL范围内,峰面积的常用对数与进样量浓度的常用对数呈良好的线性关系,R^2=1(n=5),平均回收率为99.24%~99.51%,RSD在0.05%~0.28%。此方法分析时间短,样品前处理简便、定量结果准确,重现性好,结果满意,为其质量控制提供了依据。     

猪毛色遗传的研究进展

本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。     

商会自治的基石——商会自治规范研究

在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。     

Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF

Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo.     

中华人民共和国农业部公告 第267号

为贯彻落实《兽药生产质量管理规范》(简称《兽药GMP》),进一步推动兽药GMP实施进程,我部制定了《兽药生产质量管理规范检查验收办法》,现予公告。本公告自2003年6月1日起施行。附件:兽药生产质量管理规范检查验收办法二○○三年四月十日第一章 总则 第一条 为推动《兽药生产质量管理规范》(以下简称兽药GMP)的实施,规范兽药GMP检查验收工作,制定本办法。 第二条 农业部负责全国兽药GMP管理和检查验收工作;负责制修订兽药GMP检查验收管理规定;负责兽药GMP检查员队伍建设和监督管理工作,负责国际兽药贸易中GMP互认工作。 …     

鸡败血支原体垂直传播模式及其阻断方法

以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。     

Mechanism of Action of Probiotic Function of Lactobacilli

乳酸杆菌作为益生菌广泛用于人和动物.本文综述了乳酸杆菌改善宿主健康的机制.乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道.文章重点讨论了乳酸杆菌表面成分(表面蛋白、脂磷壁酸和肽聚糖)与肠道受体(C型凝集素受体、Toll样受体和Nod样受体),阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制.     

Comparison of 2 methods of centesis of the bursa of the biceps brachii tendon of horses

REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air.