Slot-seeding investigations. 2. Time of sowing, seed rate and row spacing of Italian ryegrass

Italian ryegrass was slot-seeded into permanent grass in two field experiments at Oxford. In the first experiment August and September proved to be the most reliable months for slot-seeding, with April as a possible alternative. A row-spacing experiment showed that the optimum distance between rows was about 30 cm; seed rate in the row had a lesser influence on increasing herbage growth. In both experiments slot-seeding did not result in any substantial change in earliness of growth or alter total herbage harvested but it did even out the seasonal distribution of growth, particularly by increasing it in the following July.     

Slot-seeding investigations 7. The effects of two grasssuppressants on the growth of white clover introduced into permanent pasture

The benefits of reducing competition from the surrounding grass sward against slot-seeded clover were measured in terms of white clover dry matter production following applications of two grass-suppressing herbicides, propyzamide and carbetamide.
Clover was slot-seeded in early June 1983 and herbicides were applied in September and October 1983. The later application was more damaging to the surrounding sward. Generally, the September spraying was more effective in encouraging white clover, especially from July of the following year. Only by September 1984 did the October applications result in higher clover yields than the September applications.
Propyzamide had the greater stimulatory effect on white clover production but it also damaged the grass more than carbetamide. Increasing the dose of each herbicide increased grass damage. However, with treatments applied in September, the loss was more than compensated for by an increase in clover and in total dry matter production at the end of the year.
The results suggest that either of these herbicides, but especially propyzamide, can be used to increase both total dry matter production from the sward and the contribution from slot-seeded white clover.     

Slot-seeding investigations. 6. A comparison of the spread and productivity of different white clover varieties introduced into permanent pasture

Seeds of six cultivars of white clover were sown in 1983 in slots in tares of permanent pasture in soil-filled tanks in spring and in small plots in the field during midsummer. The clover cultivars investigated were the large-leaved Olwin and Milk nova, the medium-leaved Grasslands Huia and Aberystwyth S100, and the small-leaved Kent Wild White and Aberystwyth S184. Seeds from an indigenous clover population were also sown in the tanks. All herbage was defoliated at three- to six-week intervals to simulate rotational grazing and assessments were made until autumn 1984.
Leaf and stolon production and spread of stolons from the slots were more rapid in Milkanova, S184, Huia and Kent than in Olwen and S100 In the tanks, harvested yields of clover leaf and petiole were largest for Milkanova and Olwen and least for the indigenous population; both Olwen and S100 had a smaller percentage of their total weight outside the slot area than had the other varieties. In the field, Milkanova greatly out yielded all other varieties and S100 yielded the least, contributing 48 and 27%, resf actively, to total herbage yield. The yield of grass differed little between varieties in tanks but in the field it tended to be higher with the smaller than with the larger-leaved varieties. Total herbage yield was largest for Olwen and Milkanova in tanks and for Milkanova in the field. The greatest weight of stolons in tanks was produced by Olwen and in the field by Olwen and Kent, but the greatest length of stolons and number of nodes in both environments occurred in the smalt-leaved varieties, especially Kent. Least length of stolons and fewest nodes in the tanks were produced by Olwen and in the field by Milkanova.
It was concluded that all the varieties investigated could be successfully slot-seeded into permanent pastures but that the choice of variety will depend on subsequent use and management of the resulting swards.     

Slot-seeding investigations. 5 The effects of simulated delayed emergence and propyzamide on the spread and growth of white clover in permanent pasture

The effects of delayed emergence of white clover (cv. Grasslands Huia) seedlings, following slot-seeding during spring and early summer 1983, were simulated by sowing seeds at intervals into slots cut in turves of permanent grassland in soil-filled tanks or in the field. The resulting spread and growth of the species were assessed during the following 15–18 months. The effects of propyzamide, a grass-suppressing herbicide (at 0·2 kg ha^-1), were also investigated in the field.
A delay of 10 or 18 days in the sowing of seeds in turves in the tanks reduced clover dry weight yield from 23 to 11 during 1983 and from 118 to 96 g (0·12 m)^-2 during 1984. Spread of stolons from the slots was also greatly delayed and effects persisted for at least a year. Increased clover growth on the earliest sown treatment more than compensated for a slightly smaller yield of grass.
In the field, yields of clover from late summer 1983 until spring 1984 closely reflected the order of emergence. Afterwards, differences were less clear-cut but the cumulative yield of clover until August 1984 of the earliest-sown treatment was twice that of a treatment sown 20 d later. Effects of delayed sowing in summer 1983 were more pronounced on stolons than on foliage growth when measured in September 1984, with significant reductions in many stolon attributes even with 8 d delay in sowing. Application of propyzamide in autumn 1983 increased clover yield during 1984 from 1·7 to 3·0 and total herbage yield from 7·1 to 8·6 t ha^-1.
The results demonstrated some of the benefits of rapid seedling emergence and of the use of a grass-suppressing herbicide, but did not indicate any interaction between the two factors.     

Slot-seeding investigations. 4. Effect of phosphate fertilizer and grass suppressant on the establishment and growth of white clover in permanent pasture

White clover (cv. Grasslands Huia) was slot-seeded in April 1982 into plots of permanent pasture of differing botanical composition and reserves of soil phosphate. In one investigation the short-term effects of placing different amounts of phosphate (P₂O₅) in different positions in relation to the slot were investigated. In another, emphasis was given to effects of a grass-suppressing herbicide, propyzamide, on herbage growth and clover contributon during the second year. Fewer seedlings established on plots with a high initial P status and abundant in Holcus lanatus and Alopecurus pratensis than on plots deficient in P₂O₅ and dominated by Agrostis capillaris and Festuca rubra. P₂O₅decreased numbers of establishing clover seedlings when applied in the slots but not when placed beneath them. Growth was increased most when placement was beneath the slot. Application of P₂O₅to plots previously supplied with it resulted in 7.2 g total above ground and 1.7 g stolon dry weight (m row)^?1compared to 1.3 and 0.1 g respectively in plots with low reserves and DO applied P₂O₅. Propyzamide greatly increased the yield and the contribution of clover in the presence of high initial P status and applied P₂O₅. Without propyzamide, clover made only a small contribution until July, averaging 7% for the whole season with little benefit from residual P. Clover growth in late summer was increased from 41 to 292 kg DM ha^?1 where P₂O₅ was applied to P-deficient plots sprayed with propyzamide in the previous autumn. The large increases in clover contribution following propyzamide application were accompanied by small decreases in total seasonal yield of grass. The proportions of H. lanatus and. F. rubra. were decreased and of A. capillaris, increased. Spread of clover from the slots was slow during the first year but was encouraged by both P₂O₅ and propyzamide during the second year. Factors requiring further investigation are discussed.     

Slot-seeding investigations. 1. Effect of level of nitrogen fertilizer and row spacing on establishment, herbage growth and quality of perennial ryegrass

Three field experiments showed that perennial ryegrass can be successfully slot-seeded into an existing Festuca-Agrostis sward. Ryegrass survival was satisfactory in all cases but was improved on a low fertility site by the application of fertilizer N, P and K in the slot at sowing.
The effect of varying inter-row spacing from 37·2 to 15 cm was measured in a 3-year experiment. In year one, involving seven cuts, slot-seeding increased total herbage dry matter harvested by a mean value of 17%; inter-row distances of 22·2-30 cm gave the optimal combination of ryegrass + old sward herbage. Differences in yield between inter-row spacings declined in the subsequent two years, as the rows of ryegrass thickened. Ryegrass digestibility (measured only in year two, from six cuts) was higher than that of the old sward; total metabolizable energy harvested from 15-cm rows was 20% higher than that from unsown controls. Increasing N input from 200 to 400 kg ha^-1, starting in year two, only produced a significant increase in total herbage harvested in year three but proved effective in increasing the ryegrass contribution from the wider spaced rows.     

Metabolomic investigations of American oysters using H-NMR spectroscopy

The Eastern oyster (Crassostrea virginica) is a useful, robust model marine organism for tissue metabolism studies. Its relatively few organs are easily delineated and there is sufficient understanding of their functions based on classical assays to support interpretation of advanced spectroscopic approaches. Here we apply high-resolution proton nuclear magnetic resonance (¹H NMR)-based metabolomic analysis to C. virginica to investigate the differences in the metabolic profile of different organ groups, and magnetic resonance imaging (MRI) to non-invasively identify the well separated organs. Metabolites were identified in perchloric acid extracts of three portions of the oyster containing: (1) adductor muscle, (2) stomach and digestive gland, and (3) mantle and gills. Osmolytes dominated the metabolome in all three organ blocks with decreasing concentration as follows: betaine > taurine > proline > glycine > ß-alanine > hypotaurine. Mitochondrial metabolism appeared most pronounced in the adductor muscle with elevated levels of carnitine facilitating ß-oxidation, and ATP, and phosphoarginine synthesis, while glycogen was elevated in the mantle/gills and stomach/digestive gland. A biochemical schematic is presented that relates metabolites to biochemical pathways correlated with physiological organ functions. This study identifies metabolites and corresponding ¹H NMR peak assignments for future NMR-based metabolomic studies in oysters.     

Storage of potato tubers under water. Preliminary investigations

Summary Potato tubers stored under water at 1°C do not accumulate sugars. The addition of aseptic agents (hydrogen peroxide, thymol) allows them to be stored without any microbial spoilage. During storage for up to 8 weeks the tubers were acceptable as a food product. Because of the possibility of the practical application of this method of storage, it is planned to investigate it in full detail.