Effect of Nitrogen Fertilization and Season of Growth on Nitrate Content of Spinach Plants (Spinacia oleracea,L.)

A pot experiment was carried out in two seasons, winter and spring, using three nitrogen sources, ammonium sulphate, calcium nitrate and urea, in five rates of application, 0,25,50, 100 and 150mg N/Kg soil. Spring sown spinach outyielded the winter plants and accumulated higher amounts of N. Winter plants contained more protein. Source of N had no effect on yield or the plants content of any of the studied N-fractions. The first N-dose increased the yield over the control. Protein and water soluble N content increased with the successive N-additions up to 100 mg N/Kg soil. Most of the soluble-N was accumulated in spinach in the nitrate form. The first N-dose increased NO, % to 3 fold over the control. The nitrate content increased also with every yield increment. Petioles contained higher amounts of nitrate than leaf blades. There was a linear relationship between total and nitrate-N.     

Combined Effects of Water and Nitrogen on Growth,Biomass, and Quality of Spinach (Spinacia oleracea Linn.)

Water scarcity and nitrate contamination have caused considerable attention to environmental matters. Water and nitrogen interactions have critical impacts on their use efficiency, plant growth, and quality. In a field experiment, a combination of three water treatments and three nitrogen rates was applied to determine their interactive effects on the growth of spinach. Soil water supply that was too low [W₃N₁ (the combination of water treatment 3 and nitrogen treatment 1), W₃N₂] could cause an increase in nitrate content. Oxalate contents would increase when water and nitrogen were either inadequate (W₃N₀, W₃N₁) or too high (W₂N₂). The most profit from spinach was obtained in plots that received water treatment 2 and nitrogen fertilizer 78 kg N ha^?1. However, considering nitrogen treatments could affect the nitrate and oxalic acid, application of water treatment 2 and 39 kg N ha^?1 nitrogen fertilizer could get better spinach quality.     

Growth Irradiance Effects on Productivity,Photosynthesis, Nitrate Accumulation and Assimilation of Aeroponically Grown Brassica alboglabra

Brassica alboglabra plants were first grown aeroponically with full nutrients under full sunlight with average midday photosynthetic photon flux density (PPFD) of 1200 μmol m^?2 s^?1. Thirty days after transplanting, plants were respectively, subjected to 10 days of average midday PPFD of 1200 (control, L1), 600 (L2) and 300 μmol m^?2 s^?1 (L3). Productivity, photosynthetic CO₂ assimilation and stomatal conductance were significantly lower in low-light (L2 and L3) plants than in high-light (L1) plants. Low light plants had the highest nitrate (NO₃^?) accumulation in the petioles. Low light also had an inverse effect total reduced N content. After different light treatments, all plants were re-exposed to another 10 days of full sunlight. Low-light plants demonstrated their ability to recover their photosynthetic rate, enhance productivity and reduce the NO₃^? concentration. These results have led to the recommendation of not harvesting this popular vegetable during or immediately after cloudy weather conditions.     

Influence of Organic Manure and Lime on Cadmium Mobility in Soil and Uptake by Spinach (Spinacia oleracea L.)

Spinach grown in cadmium (Cd)-contaminated soils accumulates Cd compounds in toxic concentration contaminating the food chain leading to the chronic toxic effects on human and animal health. A study was conducted in a Cd-contaminated soil to examine the ameliorative effect of lime and organic manure on the mobility of Cd and its uptake in spinach. Application of lime or organic manure or both decreased the Cd concentration in soil and shoots and increased chlorophyll content of leaves. As compared to the control treatment, combined application of lime and organic manure decreased the level of Cd in soil by 54.7%, in spinach shoot by 61.3%, and the transfer factor of Cd by 35.0% and increased in leaf chlorophyll content by 29.4%. Combined application of lime and organic manure emerged as a viable option in reducing the mobility of Cd in contaminated soil for growing spinach.     

Effects of Water and Nitrogen Supply on Spinach(Spinacia oleracea L.) Growth and SoilMineral N Residues

Effects of conventional and optimized water and nitrogen managements on spinach (Spinacia oleracea L.)growth and soil mineral N (Nmin) residues were compared in an open field experiment in which water balance method and N recommendation with the KNS-system were included. It was shown that the conventional water treatment (seasonal irrigated amount: 175 mm) reduced spinach growth compared to the water balance treatments (seasonal irrigated amount: 80 and 85 mm) at the same N supply level due to N loss through leaching caused by excessive water supply. Although 309 kg N ha-1 was applied in the conventional N treatment, compared to 82 and 66 kg N ha-1 in the optimum N treatments, no significant difference in crop yield was investigated between the N treatments with the same irrigation practice. N uptake in spinach and soil residual Nmin contents were also significantly affected by the irrigation practices. The conventional water supply not only decreased water use efficiency, but also resulted in excessive NO-3-N being leached below the root zone. In order to meet the same target value of N requirement for the next crop, cauliflower, based on the KNS-system, at least extra 50 kg N ha-1 was needed in the conventional water treatments in comparison to the water balance treatment.     

菠菜硝态氮累积和还原与植株生长的关系

通过盆栽试验,以两个硝态氮含量差异显著的菠菜品种为供试材料,在不同生长时期,测定了叶柄、叶片干重、水分含量、硝态氮含量及叶片内源和外源硝酸还原酶活性,研究菠菜硝态氮累积和硝酸还原酶活性的动态变化及其与植株生长变化的关系。结果表明,随生长期后移,叶柄、叶片及地上部干重和水分含量先增加而后降低,硝态氮含量则持续降低,低硝态氮累积品种S9的下降更为明显,出苗后52d和62d地上部分别降低了100%和89.7%;叶片内源和外源酶活性则随植株生长量增加而增加,高硝态氮累积品种S4增加（379%和199%）更明显,之后该品种酶活性随植株生长量降低而显著下降,品种S9却显著增加,分别为121%和288%。生长前期,品种S4硝态氮含量、干重增长速率及内源、外源酶活性均显著高于品种S9,内源/外源酶活性比值却明显低于后者;生长后期,除外源酶活性和内源/外源酶活性比值外,品种间差异均不明显。因此,生长前期高累积品种硝态氮含量降低较少,主要原因可能是其内源/外源酶活性比值（70.7%）较低,生长后期该品种的内源/外源酶活性比值（98.2%）显著增加后,硝态氮含量迅速下降进一步证明了这一推测。综合上述结果可知,内源/外源酶活性比值更能揭示植株生长变化引起的品种间硝态氮含量变化差异。     

Effects of Nitrogen and Calcium Nutrition Withdrawal Before Harvesting on Biomass and Nutritive and Safety Quality in Spinach (Spinacia Oleracea L.)

A hydroponic culture was used to study different nitrogen and calcium supply patterns on biomass, nutritive, and safety quality of spinach seven days before harvesting. The results showed that the complete or partial withdrawal of nitrogen (N) and calcium (Ca) nutrient decreased spinach biomass compared to control (CK). At the same time, the withdrawal of N and Ca supply before harvesting increased vitamin C contents and decreased nitrate and oxalate content in edible parts of spinach. The decrease in spinach biomass could be partially compensated by the increase of the nutritive and safety quality of spinach and the reduced use of N and Ca nutrients.     

锶对菠菜幼苗生长、光合和抗氧化酶活性的影响

为探究锶对植物生长生理的影响,以菠菜幼苗为试验材料,研究不同浓度(0、0.1、0.5和2.5 mmol·L^-1)锶处理对菠菜幼苗的叶片鲜重、叶片锶浓度、光合指标和抗氧化酶活性的影响。结果表明,0.1 mmol·L^-1 SrCl₂处理对菠菜幼苗生长和各项生理指标均未产生明显影响,叶片锶浓度在35 mg·kg^-1左右。0.5 mmol·L^-1 SrCl₂处理对菠菜幼苗生长表现为先促进后抑制;处理20 d后,抗氧化酶活性明显上升,丙二醛(MDA)、净光合速率(Pn)和叶片鲜重相较于对照组分别增加了24%和降低了15%、10%,叶片锶浓度达到318.33 mg·kg^-1。2.5 mmol·L^-1 SrCl₂处理下,植株生长受到明显抑制,抗氧化酶系统的响应更为强烈,处理20 d后MDA增加了68%,Pn和叶片鲜重分别降低了30%和48%,叶片锶浓度达到857.51 mg·kg^-1。综上,低浓度锶处理对菠菜生长未产生明显影响,但随着锶处理浓度的增加和胁迫时间的延长,植物生长受到的胁迫作用加剧。本研究为明确锶对菠菜生长生理的调控机理提供了理论依据。     

施肥与灌水对硝态氮在土壤中残留的影响

通过田间试验研究不同施氮量与灌水量对春玉米和冬小麦田土壤中硝态氮分布与累积的影响,结果表明,春玉米收获后0~2 m土壤中累积硝态氮185.7~748.0 kg/hm2,其中1 m以上占57.9%~70.1%。由于施用氮肥而增加的硝态氮占施N量的1.8%(N 112.5 kg/hm2),50.7%(N 225 kg/hm2),56.7%(N 337.5 kg/hm2)和77.0%(N450 kg/hm2)。不施N和施N 112.5 kg/hm2时春玉米田土壤剖面没有明显累积峰;施N等于或高于225 kg/hm2时在60~80 cm土层有明显累积峰,施氮量高的峰值较高;施N 450 kg/hm2时在120~140 cm深度出现另一个累积高峰。冬小麦收获后土壤0~2 m硝态氮累积量为74.9~328.8 kg/hm2,其中1m以上占67.8%~90.7%。由于施用氮肥而增加的硝态氮占施N量的19.5%(N 112.5 kg/hm2),35.6%(N 225 kg/hm2),58.9%(N 337.5 kg/hm2)和56.4%(N 450 kg/hm2)。冬小麦田收获后土壤深层(1~2 m)没有明显的硝态氮累积,即使施氮量高达450 kg/hm2时也只在表层40 cm以上累积较多。不论是春玉米还是冬小麦,当生育期施氮量大于225 kg/hm2时0~2 m土层均有明显的硝态氮累积,施氮量高的累积量较高。施氮量是造成土壤中硝酸盐累积的主要因素,灌水量对春玉米田硝态氮的向下迁移有显著影响。     

施肥对菠菜产量和硝酸盐含量的影响

应用二次通用回归旋转组合设计,以N、P、K、有机肥料为试验因素,建立菠菜产量和NO3--N含量的回归方程.N肥和有机肥对菠菜产量和NO3--N含量均有极显著影响,所有互作效应都不显著.为了兼顾菠菜高产和较低NO3--N含量的生产目的,施肥量为:纯N 60～90 kg/hm2,P2Os 15～30kg/hm2,K2O45～67.5 kg/hm2,有机肥1125～2250 kg/hm2,预计产量和NO3--N量分别为:29104.9 kg/hm2≤Y≤33607.9 kg/hm2,2523 mg/kg≤YNO3-≤3461 mg/kg.     

Nitrate/Gly ratios in nutrition influenced the growth and amino acid composition in Spinach (Spinacia oleracea L.)

A hydroponic experiment was conducted to study the effects of fertilization with different nitrate/glycine (Gly) ratios on spinach growth and composition of amino acids. Results showed partly replacing nitrate (NO₃^?) by Gly supplied both nitrogen and carbon nutrition for spinach. Spinach accumulated the equal biomass. Total nitrogen (N) in plant had a positive relationship with nitrate/Gly ratios in nutrition. There was more than 2-fold increase in N uptake from all glycine to nitrate, and plant nitrate contents increased from 0.97 to 3.13 mg g^?1. All of 17 proteogenic amino acids in mature leaves exhibited a similar trend, the content in leaf tissue increased with an increasing ratio of nitrate/Gly, whereas relative change rate differed for each individual amino acid. In principal component analysis (PCA), the first component accounted for 80.5% of the total variance, and the N treatment appeared to be separated by this factor. In self-organizing map (SOM), 17 amino acids were divided into three clusters according to responds to nitrate/Gly ratios.     

几种新型氮肥对叶菜硝酸盐累积和土壤硝态氮淋洗的影响

应用土柱模拟试验的方法,研究了在高肥力菜田土壤条件下,施用几种新型氮肥对两茬叶菜硝酸盐积累和土壤硝态氮淋洗的影响。结果表明,在高肥力菜田土壤上,施用几种新型氮肥都未能明显提高第一茬油菜的生物量,硫硝铵（A SN）却降低了生物量,而第二茬菠菜不施肥处理生物量下降。尿素＋硝化抑制剂DM PP（En tec46）、尿素＋硝化抑制剂DCD（U＋DCD）和有机无机复混肥（OIF）3种氮肥显著降低了油菜硝酸盐含量。尿素＋玉米秸秆（U＋M S）和硫硝铵＋硝化抑制剂DM PP（En tec26）减少了土壤NO3^--N的向下淋洗,而尿素＋保水剂（U＋SAP）增加土壤NO3^--N的向下淋洗。     

土壤培肥过程中氯离子累积与分布规律研究

以陕西杨凌杜寨村为中心,沿南、西、北3条辐射线分别采取耕层和剖面土样,探讨土壤中Cl^-的累积与垂直分布情况,揭示受人类生活影响历史较长的杜寨村农田土壤中Cl^-的累积状况与迁移规律。结果表明,在半干旱偏湿润气候地区,由于受自然环境因素和人为因素的强烈影响,Cl^-在村庄附近的土壤耕层呈现出累积趋势,随着离村庄距离的增加而明显减少;土壤剖面上Cl^-的含量随着土壤深度的增加也逐渐减少;Cl^-在土壤耕层虽然表现出一定的累积趋势,但累积量并不高,暂时不会影响作物的产量和品质。     

Integrated Use of Nitrogen Fertilizer and Fish Manure: Effects on the Growth and Chemical Composition of Spinach

ABSTRACT

Combinations of inorganic nitrogen and biological fertilizers are currently advised for integrated nutrient management. To prevent agricultural fish waste from polluting water sources, recycling of the organic waste of fish manure is advised. This study was conducted to determine effect of combinations of ammonium nitrate and fish manure on yield and spinach content under greenhouse conditions. Treatments consisted of fish manure (FM), ammonium nitrate (N) and the combination of fish manure and commercial fertilizer, with four doses of nitrogen (0, 100, 150 and 200 kg N ha^?1). Applications significantly affected leaf and root fresh and dry weights, nutrient element, antioxidant enzymes and the flavonoid content of spinach. Plant growth, chemical composition and yield were superior in treatments with 150–200 kg ha^?1 nitrogen doses with the combination of FM and N. FM application with N treatment provided maximum spinach yield and plant growth by reducing the use of commercial N fertilizer.     

滨海盐碱地不同施肥模式对棉花氮磷养分积累和产量的影响

为探索盐碱地棉花种植最佳施肥模式,在山东省东营市中度滨海盐碱地设置了6种施肥模式(包括不施肥对照、习惯施肥、优化施肥、一次性施肥、减量化肥以及减量化肥+有机肥),通过对棉花关键生育时期植株调查取样和测定分析,探讨了不同施肥模式对棉花养分积累及产量的影响。结果表明:一次性施肥处理(非控释氮和控释氮1∶2配合)与同等养分水平的优化施肥处理相比,棉花的干物质积累量和各器官氮磷含量均有显著提高,前者花铃期氮磷养分总积累量分别比优化施肥处理高23.39%和13.97%,比不施肥处理高50.98%和46.94%。减量化肥(N、P_2O_5、K_2O减量20%)+有机肥处理棉花产量最高,比不施肥处理高31.14%,比习惯施肥处理高13.37%,经济效益增长1 925元/hm~2;一次性施肥处理棉花产量与习惯施肥相近,经济效益比习惯施肥增长了279元/hm~2。籽棉产量与吐絮期土壤碱解氮、有效磷含量呈显著正相关。在中度滨海盐碱地采用减量化肥(N、P_2O_5、K_2O减量20%)+有机肥和一次性施肥处理措施节本增产效果较好。     

Effects of Ammonium Nitrate and Monosodium Glutamate in Waste Water on the Growth,Antioxidant Activity,and Nitrogen Assimilation of Lettuce (Lactuca sativa L.)

It has been hypothesized that the uptake of organic as opposed to inorganic nitrogen compounds found in wastewater can be properly substituted for plant nutrients. The objective of this study was to compare effects of applying monosodium glutamate wastewater (MGW) and ammonium nitrate (NH₄NO₃) (AN) on nitrogen metabolism and growth of lettuce. The results showed that while NH₄NO₃ (AN), NO₃^-, nitrite content and NR activity increased the protein content of lettuce. Applying MGW with a high concentration of 17 amino acids and macro- and microelements improved the fresh weights of shoot and root as well as the protein content of lettuce. Antioxidant activities were not affected by AN and MGW, and their interaction effects only increased POD after 8 weeks. In conclusion, substituting a portion of the chemical fertilizers with MGW improved lettuce growth, but did not increase NO₃^- accumulation in leaves.     

Accumulation and Primary Metabolism of Nitrate in Lettuce (Lactuca Sativa L. Var. Youmaicai) Grown Under Three Different Light Sources

Light-emitting diodes (LEDs) hold great potential as electric light source for leafy vegetable production in controlled environment. However, most of LED lights in vegetable cultivation were only combined with red and blue LEDs, and the spectral composition was oversample. Previous studies reported that light spectral composition could influence nutritional quality of vegetables, e.g., nitrate content. In order to better understand the influence of spectral composition on nitrate accumulation of leafy vegetables grown in closed-type system. The nitrate content, nitrate primary metabolism of 35-day-old lettuce (Lactuca sativa L. Var. youmaicai) in vivo and its biomass were investigated. The results showed that cool white fluorescent light (T5) and high-pressure sodium light (HPS) with wide spectrum significantly reduced nitrate content in lettuce than red and blue combination LED light (RB-LED) treatment. Our findings demonstrated that a proper optimizing procedure of light spectrum with supplemental other wavebands in RB-LEDs is necessary for reducing nitrate accumulation of lettuce.     

Zinc Uptake by Spinach (Spinacia oleracea L.) as Affected by Zn Application Rate,Zeolite, and Vermicompost

Zinc deficiency in calcareous soils is a serious problem, which may be ameliorated by the application of some soil amendments. A completely randomized factorial experiment was done to investigate the effect of zeolite, vermicompost, zeolite + vermicompost, and Zn application to a calcareous soil on Zn availability, dry weight of spinach (Spinacia oleracea L.), and Zn concen-tration in spinach. Results indicated that zeolite had no effect on soil Zn availability, dry weight, and Zn concentration in spinach. Vermicompost significantly increased dry weight (1.33 g pot^?1) and decreased Zn concentration in spinach (16 mg kg^?1). Application of zeolite + vermicompost significantly increased dry weight (by 2.8 times) and Zn uptake in spinach and it also decreased Zn concentration in spinach less than vermicompost. Zinc application increased Zn concentration in spinach (up to 496 mg kg^?1), but did not affect dry weight except in soils treated with zeolite + vermicompost. Generally, application of zeolite + vermicompost is recommended instead of separate zeolite or vermicompost application for improvement of soil Zn fertility and Zn uptake by spinach.     

Effects of Soil Application of Amino Acids,Ammonium, and Nitrate on Nutrient Accumulation and Growth Characteristics of Sweet Basil

ABSTRACT

The use of organic or reduced form of nitrogen (N) can have various beneficial effects in terms of plant nutrient uptake, metabolism, and environmental issues. In this study, the influence of soil application of reduced N-forms (ammonium, glycine, and glutamine) compared to nitrate and a no fertilizer treatment was evaluated on growth characteristics of sweet basil (Occimum basilicum L.) under a moderate lime soil conditions. The basil growth traits including root and shoot biomass were increased under application of reduced N-forms mainly glycine and glutamine compared to no fertilizer treatment. Application of reduced forms of nitrogen (ammonium, glycine, and glutamine) increased the leaf concentrations of potassium (K), magnesium (Mg), calcium (Ca), iron (Fe) and zinc (Zn), whereas the leaf N concentration was increased by ammonium and nitrate fertilization compared to unfertilized control plants. The results indicate that soil application of reduced N-forms particularly glycine and glutamine is superior to nitrate application.     

茄子/大葱间作及氮肥调控对植株硝酸盐含量及养分吸收的影响

作物根系分布深度不同,其吸收养分的土壤区域也有所不同。采用大田试验方法,选择深根系的茄子和浅根系的大葱进行间作种植,并辅以氮素调控措施,研究其对蔬菜硝酸盐含量及养分吸收的影响。研究表明,无论是常规施肥还是减量施肥,间作均能降低蔬菜体内硝酸盐含量。间作区茄子的生物量和吸氮、磷、钾量均高于单作区,而间作区大葱的生物量及吸氮、磷、钾量均有所降低。说明在这一间作模式中,茄子处于优势地位,而大葱处于劣势地位。