Genetic manipulation for enhancing calcium content in potato tuber

Increased calcium (Ca) in potatoes may increase the production rate by enhancing tuber quality and storability. Additionally, increased Ca levels in important agricultural crops may help ameliorate the incidence of osteoporosis. However, the capacity to alter Ca levels in potato tubers through genetic manipulations has not been previously addressed. Here we demonstrate that potato tubers expressing the Arabidopsis H+/Ca2+ transporter sCAX1 (N-terminal autoinhibitory domain truncated version of CAtion eXchanger 1) contain up to 3-fold more Ca than wild-type tubers. The increased Ca appears to be distributed throughout the tuber. The sCAX1-expressing potatoes have normally undergone the tuber/plant/tuber cycle for three generations; the trait appeared stable through successive generations. The expression of sCAX1 does not appear to alter potato growth and development. Furthermore, increased Ca levels in sCAX1-expressing tubers do not appear to alter tuber morphology or yield. Given the preponderance of potato consumption worldwide, these transgenic plants may be a means of marginally increasing Ca intake levels in the population. To our knowledge, this study represents the first attempts to use biotechnology to increase the Ca content of potatoes.     

Optimizing crop geometry for processing-grade tuber yield,quality and economics of potato in Grey Terrace Soil

Crop geometry has great influence on economically important characteristics such as total yield, processing-grade yield, tuber size distribution and tuber quality in Grey Terrace Soil. An experiment was conducted to develop a suitable crop geometry for the production of higher processing-grade yield and quality of potato tubers. The treatment consisted of three levels of inter-row spacing (60, 67.5 and 75 cm) and four levels of intra-row spacing (20, 25, 30 and 35 cm). Crop geometry significantly influenced growth, yield contributing characters, tuber size distribution and yield of potato. The highest chips, French fry grade tubers and total potato tuber yield were found in 67.5 cm × 25 cm crop geometry without affecting processing quality but all were in the highly acceptable range. Intra-row spacing of 25 cm produced the maximum processing quality tubers, closely followed by 30 cm. The highest gross and net returns with a benefit–cost ratio of 1.75 were recorded in 67.5 cm × 25 cm crop geometry. Therefore, the 67.5 cm × 25 cm crop geometry can be recommended for higher potato tuber yield and processing-grade tubers as well as higher economic return.     

Potato tuber yield and quality in response to different nitrogen fertilizer application rates under two split doses in an irrigated sandy loam soil

Abstract

Management strategies to minimize nitrogen (N) losses to the atmosphere and water bodies from potato production fields while maintaining tuber yields and quality relies on good N management. A 2-year (2016–17 and 2017–18) field trial with ‘Symphonia’ potato was completed on a sandy loam soil irrigated with flood irrigation in Punjab, Pakistan to investigate the effect of N fertilizer rate on vegetative, yield and tuber quality parameters. The N fertilizer treatments comprising six N rates from 0 to 300?kg ha^?1 were applied at 50?kg N increments. Number of stems and tubers plant^?1 showed a quadratic response while other parameters revealed cubic trends in response to N fertilizer rates. Applying more than 250?kg ha^?1 of N fertilizer did not increase vegetative growth and yield. In conclusion, the optimal N-application rate of 250?Kg ha^?1 has great potential to improve yield and quality of potato in the sub-tropical region of Punjab, Pakistan. These findings, besides improving productivity can minimize the risk of N fertilizer loss to the atmosphere.     

Effect of location on starch content and tuber texture characteristics in potato breeding lines and cultivars

The texture of boiled potato tubers is one of the most important quality traits. The variation of tuber texture is usually examined in relationship with dry matter or starch content. The objective of the work was to examine variation and stability of texture and starch content in tubers grown in various environments. Both traits were evaluated for the set of potato breeding lines and 10 table potato cultivars. The lines were developed in the program of parental lines carried out in M?ochów Research Center, Poland. The environments were represented by three locations, which differed in cultivation system as well as climatic and soil conditions. The traits were influenced by the effects of genotype, location and their interactions. The effect of location on texture was not explained by the changes in starch content. The calculated stability parameters indicated that unstable expression of both traits was more pronounced and more frequent in breeding lines than in the cultivars. The short period of selection used in the development of the parental lines is not conducive to select potato genotypes with stable expression of quality traits. The starch content was at most moderately related with various characteristics of tuber texture.     

钾肥基施利于甘薯块根产量的形成

【目的】 研究不同施钾方法对甘薯块根产量形成的影响,可为甘薯高产稳产寻找合理的钾肥施用方法,并阐明其增产机理。【方法】 选取典型的食用型甘薯品种红香蕉和北京553为试材,于2013~2014年两个生长季在山东农业大学农学试验站进行田间试验。设4个施钾处理:不施钾肥（CK）、全部钾肥基施（JS）、全部钾肥封垄期追施（FS）、基施钾肥和封垄期追施钾肥各占50%（1/₂ JS+1/₂ FS）。在主茎第4、5片展开叶上标记13CO₂,24、48和96 h后取样,用质谱仪（Isoprime 100）测定不同部位的13C含量。从甘薯栽秧后50 d开始,每隔20 d取样直到收获。按根、茎、叶、柄四部分分别称重,将主茎自功能叶所在位置至基部等分为三段,取上、下两端用于测定蔗糖含量。收获期测定生物产量和块根产量,计算经济系数。【结果】 甘薯施用钾肥能显著提高生物产量、经济系数和块根产量,增产21.33%~34.38%。各施钾处理之间比较,生物产量差异不显著,经济系数和块根产量差异显著,其中基施钾肥处理的经济系数和块根产量最高。栽秧后50 d,基施钾肥处理植株干物质积累量和功能叶光合产物在块根中的分配率都显著高于其他施钾处理。栽秧后50 d和110 d,基施钾肥处理光合产物源端装载和库端卸载的效率显著高于其他处理,栽秧后150 d,所有施钾肥处理趋于一致。栽秧后50 d,基施钾肥处理光合产物转运效率显著高于其他施钾处理,栽秧后100 d和150 d,施钾肥处理间效果趋于一致。在栽秧后50~130 d,基施钾肥处理的块根膨大速率一直高于其他施钾处理。【结论】 在相同供试土壤和气候条件下,钾肥施用时间越早,越有利于甘薯早发、快长,促进块根早形成、快膨大;光合产物由叶片向块根运转效率的高值持续期越长,越有利于提高光合产物在块根中的分配。因此,全部钾肥基施甘薯经济系数和块根产量最高,是最经济有效的施肥方法。     

Critical potassium concentration and potassium/ calcium plus magnesium ratio in potato petioles associated with maximum tuber yields

In order to establish critical potassium (K) concentration levels and K/calcium (Ca) plus magnesium (Mg) [K/Ca+Mg] ratios in potato petioles associated to maximum total tuber yields, an experiment was conducted under Brazilian conditions. Six K levels (0, 60, 120, 240, 480, and 960 kg K₂O/ha as potassium sulphate) were applied in a randomized complete block design experiment with four replications. Baraka potato tubers were seeded, spaced 0.8 x 0.3 m, following the agronomic cultural practices recommended for the crop. After 48 days from plant emergence, plant tissue samples was collected from each plant, the youngest fully expanded leaves (YFEL) and the oldest but not senescent leaves (ONSL). Petioles from these leaves were analysed to their K, Ca, and Mg contents. At harvest, total, high grade, and weight tuber yields were increased to 733, 719, and 660 g/plant for the 353, 335, and 384 kg K₂O/ha levels, respectively. These yields are double the Brazilian potato yield average. Petiole Ca and Mg concentrations were decreased by the K fertilizer treatments, whereas the opposite occurred with the K/Ca+Mg ratio. Potassium concentrations in the petioles from the YFEL and ONSL increased up to 10.44 and 7.13 g K/100 g petiole dry matter at the 672 and 654 kg K₂O/ha levels, respectively. The K petiole gradient concentration was not affected by K fertilization. The K/Ca+Mg ratio for YFEL associated with the maximum total tuber yield was 7.24, while the K concentrations were 8.91 and 6.16 g K/100 g dry matter in petioles for the YFEL and ONSL, respectively.     

化肥和生物有机肥配施对鲜食型甘薯块根产量、品质及土壤肥力的影响

  【目的】  研究生物有机肥和化肥对鲜食型甘薯干物质积累、产量品质及土壤肥力的影响,为鲜食型甘薯的优质高效生产提供技术支撑。  【方法】  2018和2019年,以鲜食型主栽品种‘济薯26’为供试品种,在山东济南丘陵区进行了两年定位田间试验。设置不施肥(对照)、单施化肥、单施生物有机肥、化肥生物有机肥配施处理。在移栽后50、100和150天,取样分析植株干物质积累和分配。于成熟期,调查分析块根产量及品质,以及0—20 cm土层土壤肥力指标。  【结果】  与单施化肥相比,单施生物有机肥和化肥生物有机肥配合处理均显著提高了两年度移栽后150天甘薯植株干重和块根干重,块根产量以化肥配合生物有机肥处理最高,单施生物有机肥次之,均显著高于单施化肥。与单施化肥相比,单施生物有机肥可显著提高两个年度土壤脱氢酶和蔗糖酶活性,显著提高第二年度土壤脲酶和碱性磷酸酶活性及土壤速效磷、速效钾和有机质含量,促进干物质向块根分配,显著提高了第二年度块根还原糖、可溶性糖和维生素C含量。与施用生物有机肥相比,化肥生物有机肥配施显著提高了两个年度移栽后100天和150天植株干重和块根干重,显著提高第二年度土壤碱性磷酸酶和脱氢酶活性,提高土壤碱解氮和速效磷含量,还原糖和可溶性糖含量无显著变化,但显著增加类胡萝卜素和维生素C含量。相关分析表明,块根品质指标与土壤有机质、速效磷和速效钾含量显著正相关,与土壤酶活性亦有显著正相关关系。  【结论】  连续进行化肥与生物有机肥配施,可显著提高土壤碱性磷酸酶和脱氢酶活性,增加土壤碱解氮和速效磷含量,促进甘薯植株和块根干物质的持续积累,获得高产,同时保持较高的块根糖含量和维生素C含量,是提高鲜食型甘薯产量和品质的有效措施。     

Effects of different nitrogen forms on potato growth and development

The objective of this study was to test if the effects of different nitrogen forms on potato growth depend on the plant growth stage. Plants from different potato cultivars were treated with different forms of nitrogen before tuber initiation and after tuber formation. A nitrification inhibitor was used to prevent the transformation of ammonium (NH₄⁺) to nitrate (NO₃^?). Plant growth, tuber formation, leaf area, leaf chlorophyll content, and tuber yield were assessed. The results obtained over 2 years indicate that plants treated with NO₃-nitrogen (N) before or at tuber initiation produced more tubers per plant than those treated with NH₄-N. However, plants treated with NH₄-N develop tubers earlier. Additionally, after tuber formation, plants treated with NH₄-N had better shoot growth than plants treated with NO₃-N. A larger leaf area with higher leaf chlorophyll content resulted in greater dry matter accumulation and higher tuber yield at harvest for plants treated with NH₄-N.     

Effect of Integrated Management of Nitrogen Fertilizer and Cattle Manure on the Leaf Chlorophyll,Yield, and Tuber Glycoalkaloids of Agria Potato

Management of nutrients, especially nitrogen (N), is one of the most important factors in potato production. Cattle manure and mineral fertilizers are two sources of N that can affect the quality and quantity of potato yield. The effects of the use of cattle manure (5, 10, 15, and 20 ton ha^?1), N fertilizer (50, 100, and 150 kg ha^?1), and their interaction on tuber yield, chlorophyll content, and glycoalkaloid concentration were evaluated during field experiments in Iran in 2008 with a randomized complete block design with a factorial arrangement of three experimental replications. At the time of flowering, chlorophyll a, b, and total (chlorophyll) were recorded by spectrophotometry. Furthermore, at the end of the growth season, tuber yield was calculated and tuber glycoalkaloids were measured by the colorimetric method for the assessment of quantitative and qualitative characteristics of potato. Chlorophyll a, b, and total content increased linearly and very significantly in response to the application of manure and N fertilizer. The interaction between manure and N fertilizer was also significant; somehow the maximum content of total chlorophyll [1.448 mg g^?1 fresh weight (FW)] was obtained by using 150 kg N + 20 ton of manure per hectare. Cattle manure, N fertilizer, and their combination had a highly significant effect on tuber yield. Maximum tuber yield (36.8 ton ha^?1) was obtained by the utilization of 20 ton manure + 150 kg N per hectare. Total glycoalkaloid content was affected by the N application only. It showed a linear increase in the presence of increased concentration of N fertilizer.     

Einfluß des Calcium-Angebotes auf Wachstumsrate und Calcium-Gehalt von Kartoffelknollen

Effect of calcium supply on growth rate and calcium content of potato tubers. . Using potato plants in water culture with a constant calcium supply to the roots, a study was made of the influence of additional supply of calcium to the tuber surface on growth rate and calcium content of the tubers. Under conditions of high relative humidity around the tubers the calcium nutrition of the tubers was exclusively dependent on the calcium supplied to the tuber surface (?exogenous calcium”?). With a high level of exogenous Ca the growth rate of the tubers was high and the rate of uptake of Ca was proportional to the tuber growth. With a low level of exogenous Ca the growth rate declined to a lower but constant value. In absence of exogenous supply of Ca the growth rate of the tubers declined gradually until growth ceased. Cessation of tuber growth occurred when the Ca content decreased below 0,36 mg Ca per g dry matter for smaller tubers and below 0,14 mg Ca per g dry matter for larger tubers. Severe Ca deficiency in potato tubers causes necrosis at the tuber apex.     

Influence of arbuscular mycorrhizal fungi on storage metabolites,mineral nutrition,and nitrogen-assimilating enzymes in potato (Solanum tuberosum L.) plant

Mycorrhizae are fungal symbionts forming mutualistic relationship with plant roots. In this study, surface-sterilized potato tubers were sown in earthen pots filled with sterile soil. Half of the pots were inoculated with sterilized arbuscular mycorrhizal fungi (AMF) spores and sterilized inoculated maize root fragments, and another half without any AMF inoculation representing control. Inoculation was done twice 3 days before sowing the tubers and on the onset of seedling emergence. Plants, along with their controls, were sampled at 20-day intervals upto 80 days after tuber initiation. The tubers of potato are shown to present a higher level of metabolites and mineral nutrition in the mycorrhizal inoculated compared to the non-inoculated. The results showed that AMF has a potential in enhancing potato production by increasing the storage metabolites, mineral nutrition in tubers and nitrogen assimilating enzymes in plant.     

Efficiency of Nitrogen Fertilizer for Potato under Fertigation Utilizing a Nitrogen Tracer Technique

Abstract

Efficient crop use of nitrogen (N) fertilizer is critical from economic and environmental viewpoints, especially under irrigated conditions. Nitrogen fertilizer (¹⁵N‐labeled urea) and irrigation methods (drip and furrow) were evaluated on spring and fall potato cultivars under Syrian Mediterranean climatic conditions. Field experiments were conducted in the El‐Ghab Valley near Hama in fall 2000 and spring 2001 on a heavy clay soil. Four N‐fertilizer applications (70, 140, 210, and 280 kg N/ha) were applied in five equally split treatments for both irrigation methods. Potato was irrigated when soil moisture in the specified active root depth reached 80% of the field capacity as indicated by the neutron probe.

Higher marketable tuber yield of spring potato was obtained by fertigation compared to furrow irrigation; the magnitude of tuber yield increases was 4, 2, 31, and 13%, whereas for fall potato the tuber yield increases were 13, 27, 20, and 35% for N fertilizer rates of 70, 140, 210, and 280 kg N/ha, respectively. Shoot dry matter and tuber yields at the bulking stage were not good parameters to estimate marketable tuber yield. The effect of N treatments on potato yield with furrow irrigation and fertigation was limited and not significant. Drip fertigation improved tuber yield of fall potato relative to national average yield. Nitrogen uptake increased with increasing N input under both irrigation methods. Reducing N input under both irrigation methods improved N recoveries. Increasing N input significantly increased total N content in plant tissues at the bulking stage. Spring potato yields were almost double those of fall potato under both irrigation methods and all N treatments.

Nitrate (NO₃) movement in the soil solution for fall potato was monitored using soil solution extractors. Furrow irrigation resulted in greater movements of NO₃‐N below the rooting zone than drip fertigation.

Harvest index did not follow a clear trend but tended to decrease upon increasing N fertilization rates beyond 140 kg N/ha under both irrigation methods. Drip fertigation improved field water‐use efficiencies at the bulking and harvest stages. Fertigation increased specific gravity of potato tubers relative to furrow irrigation. Higher N input decreased specific gravity of potato tubers under both irrigation methods.     

Phosphorus compounds and their solubility in swine manure compost

In the Japanese warm areas, as a result of intensive and repeated potato cropping, common scab, a soil-borne disease has become a serious problem. The soil moisture level has been shown to be an important factor affecting the incidence of common scab. That is, a high level of soil moisture reduces the severity of common scab. However, in this investigation, although during the spring cropping precipitation was abundant, a large number of tubers showing scab lesions were produced. The results indicate that the soil moisture level may not be an important factor affecting the incidence of potato scab in the humid region.

To identify other factors which may affect scab severity, the relation between sugar content in the peel of potato tuber and the incidence of common scab was investigated. Generally, the amount of reducing sugar in the tuber peel increased gradually with the growth of the tubers, then reached a maximum level two weeks after the start of tuber formation, and thereafter declined. The period corresponding to the highest sugar content in peels coincided with the most susceptible period for scab infection. The content of reducing sugar in the peel of susceptible cultivars was higher than that of the resistant cultivars, throughout the tuber growth, and the critical value of the content was 2.0 mg per 100 g fresh peel. These results suggest that the content of reducing sugar in tuber peel may be an important factor affecting the incidence of common scab.     

滴施不同铵硝比例氮肥对马铃薯生长的影响

在盆栽试验条件下,以滴灌施肥方式探讨滴施不同铵硝比例氮肥对马铃薯生长、产量和品质的影响。研究结果表明:在本试验条件下,滴施不同铵硝比例氮肥对马铃薯植株生物量的积累差异不显著;滴施铵态氮肥有利于提高马铃薯块茎中N的浓度,而滴施硝态氮肥有利于提高马铃薯块茎中Ca的浓度;滴施不同铵硝比例氮肥处理马铃薯的淀粉含量、干物质含量、灰分含量等均差异不显著,块茎产量也差异不显著,但马铃薯商品薯比例以滴施铵硝比例为50/50氮肥处理的最高,达到92.01%。     

Tuber yield and allocation of nutrients and carbohydrates in potato plants as affected by limestone type and magnesium supply

Abstract

Magnesium (Mg) is a nutrient that affects the development of plants and is mainly supplied through liming performed to correct soil acidity. By acting on photosynthesis and influencing carbohydrate partitioning in the plant, supplementary Mg supplied through soil or foliar application can increase the yield and quality of potato (Solanum tuberosum L.) tubers. The aim of this study was to evaluate the effect of supplemental Mg fertilization by soil or foliar application on plant nutritional status, tuber yield, and carbohydrate partitioning in potato crops in soil corrected with calcitic and dolomitic limestones. The experiment was carried out in pots under greenhouse conditions with a randomized block design in a 2?×?3 factorial scheme with four replications. Dolomitic limestone application and supplemental Mg fertilization via soil increased the concentrations of this nutrient in potato leaves. Liming with dolomitic limestone reduced the uptake of Ca and K by plants, but supplemental Mg fertilization did not alter the uptake of Ca, Mg or K. Supplemental Mg fertilization did not increase plant growth and tuber yield, even when calcitic limestone was used to elevate the base saturation to 60%; the exchangeable Mg concentration in soil was 9?mmol_c dm^?3, and the Ca:Mg relationship was 3.7. Liming with dolomitic limestone or providing supplemental Mg fertilization did not increase sugar and starch partitioning to the tubers.     

Stärkegehalte von Kartoffelknollen in Abhängigkeit von Kalium-Ernährung und Knollenentwicklung

Starch content of potato tubers in relation to K nutrition and tuber growth In a pot experiment with potato (cv Saturna) K content in tubers increased from 1.0 to 2.6 % of tuber-DM due to increasing rates of K fertilizer. Simultaneously starch in tuber fresh matter decreased from 21 to 15 %. Highest starch content in tuber dry matter was obtained in that K treatment which gave the highest yield in dry tubers. Measuring starch accumulation during tuber growth revealed that already in the flowering stage differences exist in the starch contents of the tuber dry matter between the lowest and highest K treatments, namely 69 % for K₁ as compared with 64 % for K₅. The rate of starch accumulation between flowering and final harvest, however, was similar in all K treatments. This suggests that K supply affects the storage tissue at an early stage of tuber development probably via different water relations of tubers.     

Effects of soil fertility on growth,tuber yield,nodulation and nitrogen fixation of yam bean (Pachyrhizus erosus (L.) urban) grown on a typic eutrustox

The tuberous legume, Yam Bean, (Pachyrhizus erosus (L.) Urban, has been utilized as a food crop for many centuries. The large, starchy tubers have higher aitrogen content than potatoes, cassava and taro roots with 20% or more of the N fraction as ureidoglyco‐lates. Yam Bean (Jicama) tuber growth within the neotropical regions of the world is influenced by soil productivity. The objective of this study was to determine effects of soil fertility treatments on tuber yield, nodulation characteristics and nitrogen fixation.

Highly significant increases in growth and tuber production resulted with P additions, and to Ca and K levels when combined with P. Nodule weight and nitrogenase activity were similar in response to factorial soil treatments as were the tuber and total above ground plant growth. Total ureide content of tuber epiperi‐derm tissue increased significantly with P, Ca, and P + Ca treatments and increased quadratically with increased K additions when combined with P, Ca, and P + Ca treatments. Multiple regression for nitrogenase = 3.4 g top wt. + 3.5 g tuber wt. + 4.9 g nodule wt. + 3.7 umol ureide, R² = 0.88 and C.V. = 16.5%. The percent nonstructural tuber carbohydrates was not significantly influenced by soil treatments although total tuber N content significantly increased with the P treatments. Content of plant nutrient elements within nodule cytosol generally increased significantly with addition of the corresponding element in the soil fertility treatments. Increased K content was quadratic for increased K additions with concomitant decrease in Na content having highly significant negative correlation (r = ‐0.72). Adequate available soil P, Ca and K favorably influenced Yam Bean tuber growth and nitrogen fixation with P a first limiting factor for desirable high tuber yields.     

Effect of various phosphorus and calcium concentrations on potato seed tuber production

Although the effects caused in plants by the calcium (Ca)–phosphorus (P) interaction in calcareous soils are well documented, very few studies report on such effects in nutritive solutions or hydroponic cultivation. In a sand and perlite (1:1 volume) hydroponic system, effects of various P (21, 42, and 63 ppm) and Ca (120, 180, and 240 ppm) concentrations on potato tuberization were studied. A factorial experiment based on a completely randomized design with three replications was conducted. For maximum tuber yield and tuber number production, 21 and 42 ppm P was sufficient, respectively. Increase in P and Ca concentrations in nutrient solutions in early growing season resulted in an increase in shoot and root weight, leaf area, and shoot length linearly, but tuber yield and tuber number did not follow this trend. The maximum tuber specific gravity and total solid percent were also observed in 42 ppm P. The highest tuber number and tuber yield were observed in 120 ppm Ca concentration.     

Effect of humic acid multinutrient fertilizers on yield and nutrient use efficiency of potato

A field experiment was conducted to study the effect of humic acid multinutrient fertilizers like Grow Flow 45H and HA-NPK complex on crop yield, nutrient content and uptake, and nutrient use efficiency of potato. Application of Grow Flow 45H (humic acid multinutrient liquid fertilizer) at recommended dose increased the tuber yield by 9.3% as compared to chemical fertilizer. Nitrogen (N), phosphorus (P) and potassium (K) content of the Grow Flow 45H treated plants were 2.89, 0.33 and 1.58% in shoots and 1.89, 0.21 and 1.03% in tubers, respectively which were significantly higher than the other treatments. Grow Flow 45H increased the N, P and K use efficiencies by 16.4%, 9.3% and 18.3% respectively over chemical fertilizer. Though HA-NPK complex fertilizer (developed in the laboratory) was not significantly different from chemical fertilizer in respect of yield, the contents and uptake, and use efficiencies of N, P and K; however the former was found better than the latter.     

滴灌施氮对高垄覆膜马铃薯产量、氮素吸收及土壤硝态氮累积的影响

通过田间试验研究了高垄覆膜滴灌条件下施氮量（N 0、90、180、270、360 kg/hm2）对马铃薯产量、土壤硝态氮积累、氮素平衡及氮肥利用率的影响。结果表明,N180处理的马铃薯块茎产量最高。马铃薯收获期各处理硝态氮含量为表层土（020cm）最高,且在0120 cm剖面呈现降低的趋势;各处理040 cm土层硝态氮积累量占0120cm土层硝态氮积累总量的47.74%～53.17%。施氮量与马铃薯吸氮量、土壤硝态氮残留量、氮素表观损失量呈显著正相关,马铃薯吸氮量、硝态氮残留量和氮素表观损失量分别占增加纯氮的37.93%、45.99%和16.08%。马铃薯块茎吸氮量和收获指数随着施氮量的增加有增加的趋势;氮肥吸收利用率、氮肥农学利用效率、氮肥生理利用效率均以N 90处理最高,分别为67.97%、68.06 kg/kg和154.92 kg/kg。在内蒙古阴山北麓马铃薯主产区,覆膜滴灌施氮量应控制在90～180 kg/hm2。