Application of Biochar and Compost for Enhancement of Rice (Oryza Sativa L.) Grain Yield in Calcareous Sandy Soil

The application of compost to calcareous soils by farmers is a well-established practice and has been shown to improve yields. However, incorporation of biochar and mixture of biochar and compost into calcareous soils is a relatively novel concept for improving soil quality and yield since calcareous soils comprise a large scale of soils worldwide. The objective of this study was to determine the effects of the co-application of biochar and compost on the soil properties, nutrient status and grain yield of rice in calcareous sandy soil. The experiment was conducted in a factorial arrangement based on randomized complete block design with three replications. The compost application rates were 1% and 3% (w/w; compost/soil) and the applied rates of biochars (rice straw biochar, RSB; sugarcane bagasse biochar, SBB) were 0.3% and 0.9% (w/w; biochar/soil). The results showed that soil pH decreased with increasing application rates of either compost or biochars. However, soil EC was enhanced through increasing the application rates of compost and biochars. The co-application of biochar and compost improved soil total N and available P concentrations. The soil available K increased with increasing the rate of incorporated biochars and compost. An increase of soil available K was more predominate with the application of RSB than SBB. The RSB, also, added a considerable amount of silicon (Si) to the soil. The co-incorporation of biochars and compost enhanced soil available concentrations of Fe, Zn, Cu, and Mn as well. The RSB was more effective than the SBB in grain yield enhancement almost certainly due to a higher Si content in RSB. Furthermore, the concurrent application of biochars and compost increased grain yield more than applying them individually. A higher application rate of biochar and compost induced a higher grain yield. The co-application of highest rates of RSB (0.9%) and compost (3%) induced the highest grain yield (26.1 g/pot) among the treatments. The increase in yield compare to the control were 321% and 260% for 0.9% RSB + 3% compost and 0.9% SBB + 3% compost, respectively. The increase in the grain yield was due to an improvement in the soil chemical properties and nutrients enhancement. Finally, the co-application of the highest rate of RSB (0.9%) and compost (3%) is recommended to obtain the appropriate rate of rice grain yield in calcareous sandy soil.     

Effect of Plant Growth Promoting Rhizobacteria on Fe Acquisition in Peach (Prunus Persica L) Under Calcareous Soil Conditions

Abstract

The effects of Alcaligenes 637Ca, Agrobacterium A18, Staphylococcus MFDCa1, MFDCa2, Bacillus M3 and Pantoea FF1 were tested on peach cv. ‘Elegant Lady’ on GF677 and Nemaguard rootstocks. Average increases in citric, malic, oxalic, butyric and fumaric acid in the leaf were obtained from rhizobacterial root inoculations of 33.1, 17.5, 21.7, 26.5, and 44.7% in Elegant Lady/Nemaguard, respectively. FF1 showed the highest FC-R activity in the roots of Nemaguard (112.4?nmol?FW?gr^?1?hr^?2) and GF677 (162?nmol?FW?gr^?1?hr^?2). The highest FC-R activity in the leaf was with MFDCa2 in Elegant Lady/Nemaguard (57.1?nmol?FW?gr^?1?hr^?2) and 637Ca in Elegant Lady/GF677 (87.9?nmol?FW?gr^?1?hr^?2). PGPR strains used in this study expressed increased leaf organic acid content, FC-R activity in the roots and leaf, increased active Fe content in the leaf, and possessed a great potential for the improvement of iron availability to plants.     

Antifungal and Plant Growth Promoting Activities of Indigenous Rhizobacteria Isolated from Maize (Zea mays L.) Rhizosphere

Plant growth promoting rhizobacteria (PGPR) enhance the plant growth directly by assisting in nutrient acquisition and modulating plant hormone levels, or indirectly by decreasing the inhibitory effects of various pathogens. The aim of this study was to select effective PGPR from a series of indigenous bacterial isolates by plant growth promotion and antifungal activity assays. This study confirmed that most of the isolates from maize rhizosphere were positive for PGPR properties by in vitro tests. Azotobacter and Bacillus isolates were better phosphate solubilizers and producers of lytic enzymes, hydrocyanic acid (HCN), and siderophores than Pseudomonas. Production of indole-3-acetic acid (IAA) and antifungal activity were the highest in Azotobacter, followed by Bacillus and Pseudomonas. The most effective Azotobacter isolates (Azt₃, Azt₆, Azt₁₂) and Bacillus isolates (Bac_10, Bac₁₆) could be used as PGPR agents for improving maize productivity. Further selection of isolates will be necessary to determine their efficiency in different soils.     

Effect of Indigenous Plant Growth-Promoting Rhizobacteria on Wheat (Triticum Aestivum L.) Productivity and Soil Nutrients

The present study aimed at selection of efficient bacterial isolates with multiple plant growth-promoting (PGP) traits at variable doses of chemical fertilizers for enhanced wheat productivity and sustenance of soil health. Ten bacterial isolates from wheat (rhizosphere soil and root endosphere) were screened for PGP traits (indole acetic acid, phosphate solubilization, siderophore production, and ammonia production). Only three isolates (B2, SIR1, and BIS2) possessed all PGP traits. Net house evaluation of these isolates at graded doses of chemical fertilizers revealed that the potential of B2 isolate is significantly superior for enhancing wheat yield and soil properties. On the basis of 16S rDNA analysis, the potential isolate (B2) was identified as Serratia marcescens. Conjoint use of the B2 isolate at 80% recommended doses of fertilizers (RDF) significantly increased wheat growth and saved 18 kg nitrogen and 10 kg phosphorous on per hectare basis. The developed module not only increases profitability but also protects the environment and sustains soil health.     

植物根际促生菌促进小麦生长及提高其抗旱性的研究进展

干旱胁迫是影响小麦生长发育与产量形成最主要的非生物胁迫因子,近年来化肥的过量使用及全球气候变化产生的不利影响,导致小麦在生长过程中受到的干旱胁迫危害日趋严重。植物根际促生菌(PGPR)不仅可以协助小麦提高养分利用率,促进其生长发育,还能够通过自身作用或产生多种代谢产物协助小麦抵御干旱胁迫。本文归纳总结了PGPR促进小麦生长及提高其抗旱性的研究进展,为利用PGPR提高小麦促生抗旱的研究奠定了理论基础。     

不同施用方式下污泥堆肥对土壤性质和草坪生长的影响

为解决城市污泥处理难题,探讨污泥堆肥及其不同施入方式对绿地土壤的改良效果,进行了污泥堆肥绿地土壤改良试验。试验设混合(H)、覆盖(F)、混合+覆盖(HF)3种施入方式,结果表明:不同施入方式均能显著降低绿地土壤容重,提高土壤中有机质、碱解氮、速效钾、有效磷含量,HF和H的改良效果最好;不同施入方式还均能显著促进草坪生长和分蘖,其中HF,F,H处理的分蘖密度分别比对照高45%,37%,32%;F处理的杂草抑制率在96%以上,HF为78%,H处理未能抑制杂草;各施入方式均能显著提高土壤微生物碳和土壤微生物氮含量,改良1年后,F、H和HF的土壤微生物碳含量分别是对照2.4,3.1,3.4倍,微生物氮含量分别是对照的1.7,3.0,2.8倍;与对照相比,HF处理与H处理的土壤脲酶、酸性磷酸酶和蔗糖酶活性均显著提高。     

Effects of Root Plant Growth Promoting Rhizobacteria Inoculations on the Growth and Nutrient Content of Grapevine

The objective of this study was to evaluate the effects of seven nitrogen (N₂)-fixing and/or phosphorus (P)-solubilizing and siderophore-producing microorganism based bio-fertilizers in single and triple strain combinations isolated from the acidic rhizospheric soil of native tea, grapevine, and wild red raspberries. As a result of this study, bacterial efficiency was found to be variable and depended on the bacterial strains and evaluated growth parameters. Plant growth-promoting rhizobacteria (PGPR) has improved macro- and micro-nutrient concentrations in grapevine leaves, and stimulated plant growth. Triple inoculation and single inoculation based bio fertilizers were found to stimulate overall plant growth, including shoot and leaf weight, main shoot length, leaf ground index, chlorophyll, nitrogen, zinc and iron content of grapevine cv ‘Italy’. Bio-fertilizers increased the nutrients such as nitrogen, zinc and iron concentrations and consequently increased the chlorophyll content of the leaves.     

烟杆生物炭对砒砂岩与沙复配土壤理化性状及玉米生长的影响

为明确施用生物炭对砒砂岩与沙复配土壤水分保持及肥力提升的影响,采用盆栽试验,研究了不同生物炭施用量(0,10,20,30,50g/kg(以风干土计))对砒砂岩与沙复配土壤理化性状及玉米生长的影响。结果表明:在种植玉米一季后,施用生物炭可显著降低复配土壤容重,尤其当生物炭施用量达到30g/kg时,土壤容重可降低至1.37g/cm3,但当生物炭施用量增加到50g/kg时,土壤容重又出现增加的趋势;土壤田间持水量随生物炭施用量的增加呈显著增加趋势,但当施用量增加到50g/kg时又会出现下降趋势;土壤pH、全盐量随生物炭添加量的增加显著增加,尤其当生物炭添加量为50g/kg时,土壤pH可达8.80,全盐量可达2.51g/kg;土壤有机质、有效磷、速效钾含量也随生物炭施用量的增加而显著增加,但有效磷在生物炭施用量增加至50g/kg时出现下降趋势。进一步分析不同生物炭处理对玉米生物量的影响,发现玉米根干重、地上部分干重、百粒重、单株产量均随生物炭施用量的增加呈显著增加趋势,但当生物炭施用量增加到50g/kg时,上述各指标反而显著降低。生物炭对于砒砂岩与沙复配土壤理化性状、水分保持、肥力提升、作物生长及产量等诸多方面都有明显改善效果,在施用过程中需要注意使用量,在本试验条件下,生物炭推荐施用量为30g/kg干土。     

生物质炭施用及老化对石灰性紫色土中磺胺类抗生素迁移特征的影响

以长江上游低山丘陵区广泛分布的石灰性紫色土旱地的耕作层土壤为对象,采用室内批量平衡吸附试验和填装土柱穿透试验,研究了施用1%生物质炭及3年老化作用对2种磺胺类抗生素(磺胺嘧啶和磺胺二甲基嘧啶)吸附和迁移特征的影响。结果表明,Freundlich方程能更好地拟合抗生素在土壤中的等温吸附曲线,施用生物质炭提高了土壤对抗生素的吸附能力,吸附常数KF值依次为:老化1%施炭土>新鲜1%施炭土>对照土;土柱出流液的磺胺嘧啶和磺胺二甲基嘧啶相对浓度峰值均表现为:老化1%施炭土<新鲜1%施炭土<对照土,说明生物质炭的添加能有效减少控制紫色土中抗生素的淋失迁移,以对磺胺二甲基嘧啶的阻控效果较好;生物质炭老化3 a后土壤对抗生素的吸附作用与阻控效果均有所提高,主要归因于土壤pH的提高。     

Influence of Nitrogen and Potassium Fertilization on Yield and Quality of Rosemary in Relation to Harvest Number

Rosemary is an important aromatic and spice plant. Its cultivation for production of its essential oil has gained importance in recent years. An experiment was conducted in a red sandy loam (Kandiustalf) soil to study the impact of cultivation of this multiharvest crop at different nitrogen (N) and potassium (K) application regimes on soil fertility and crop yield at the farm of the Central Institute of Medicinal and Aromatic Plants, Resource Center, Bangalore, during 2003–2005. The treatments consisted of all combinations of N levels (0, 150, and 300 kg per hectare per year) and K levels (0, 50, and 100 kg per hectare per year). Five harvests were taken at about 5‐month intervals. The results showed that initially the soil alone was able to meet the crop requirements for K, but with the progress of time, addition of K to the soil in the form of fertilizer became necessary for obtaining optimum yields. At the end of five harvests, exchangeable K in soil was significantly lower as application of K decreased from 100 to 50 and 0 kg per hectare per year. Soil fertility could be maintained and oil yields of rosemary from four harvests could be increased from 146.6 L to 344.7 L per hectare by application of 150 kg N and 100 kg K.     

污泥堆肥对盐碱土土壤环境和作物生长的影响

以城市污水处理厂污泥堆肥为研究对象,采用盆栽试验方法研究不同用量(0%,5%,10%,20%)污泥堆肥施入盐碱土后,对玉米、大豆生长情况及土壤环境的影响。结果表明,施用污泥堆肥可降低盐碱土pH值和总盐含量,提高土壤养分及酶活,增加土壤微生物量碳、微生物量氮,同时施用污泥堆肥土壤重金属Zn、Cu含量增加,当施用量为20%时,土壤Zn含量超标。另外,玉米和大豆在污泥堆肥施用比例分别为10%和5%时长势最佳,且作物籽粒中重金属含量在国家食品卫生标准范围内。     

Effects of Food Waste Compost on Soil Microbial Populations,Tomato Yield,and Tomato Quality

A pot experiment was conducted to investigate the effects of food waste compost (FW) on soil microbial population and growth, fruit yield, and quality of tomato grown in a greenhouse compared to no fertilizer (CK), chemical fertilizer (CF), decomposed chicken manure (CM), pig manure (PM), horse manure (HM), and bull manure (BM). Results show that FW treatment had the greatest numbers of bacteria, fungi, and actinomycetes in soils and shoot biomass, fruit diameter, and fruit yield of tomato, and it increased fruit yield by 12.6, 28.5, 31.0, and 40.0% when compared to HM, CF, CM, and CK, respectively. The FW treatment also improved fruit quality, with the contents of vitamin C, soluble sugar, and organic acid and the sugar/acid ratio of 22.8 mg 100 g^?1, 3.84%, 0.50%, and 7.73, respectively. Thus food waste compost can be as an alternative to chemical fertilizer and other animal manures in vegetable cultivation.     

生物质炭浸提液对大蒜生长品质及土壤的影响

[目的]证实生物质炭中的水溶性有机碳对作物生长和品质的作用,为生物质炭的应用开辟新的途径。[方法]在实验室制取400,450,500℃小麦秸秆生物质炭,以KOH溶液浸提水溶性有机碳获得生物质炭浸提液W_(400),W_(450),W_(500),并应用于盆栽大蒜试验,研究其对大蒜生长、品质及土壤的影响。[结果](1)W_(400),W_(450),W_(500)对大蒜的生长都没有显著影响;(2)W_(400)处理使大蒜可溶性糖的含量显著提高了27.53%,土壤有机质和速效磷含量分别提高37%,26%;(3)W_(450)处理下大蒜维生素C、大蒜素含量分别显著提高34.9%和8.2%。[结论]生物质炭浸提液对大蒜生长没有影响,但可以提高大蒜品质。     

堆肥对重金属污染土壤上茼蒿品质和产量的影响

为了解堆肥施人重金属Cu、Cd污染土壤后对蔬菜品质和产量的影响,利用盆栽试验研究了不同堆肥用量下茼蒿品质、产量和重金属含量的变化。结果表明,随着堆肥用量的增加,茼蒿产量、粗蛋白、VC、P和K含量先显著增加后降低,而茼蒿可溶性糖含量呈先显著降低后增加的趋势。堆肥量的增加显著降低茼蒿中Cu含量,比对照最多降低35．5％。茼蒿地上部Cd、根部Cu和Cd含量呈先显著降低又上升的趋势。茼蒿地上部Cd含量在堆肥用量为20g·kg^-1时达最低,根部Cu、Cd含量与对照相比最多分别降低20．1％和39．5％。对于污染地区的茼蒿种植,试验堆肥的适量施用是切实可行的。     

Effect of Compost and Hydroabsorbent Polymer on Tree Growth and Soil Properties in a Tropical Urban Environment

Horticultural application of hydroabsorbent polymer (HP) has drawn research attention due to its perceived benefits to plant growth. Few studies have compared the use of compost and HP amendments on tree establishment in tropical urban environments. An experiment was conducted to assess the effect of compost (40% v v^–1) and HP (3 and 5 kg m^–3) on the growth of two native tree saplings (Calophyllum soulattri and Syzygium myrtifolium) in loamy and sandy soils. The HP treatments significantly affected soil pH and extractable phosphorus (P) and potassium (K), whereas combined application of compost and HP (5 kg m^–3) resulted in significantly higher nitrogen (N) in both soil types. Plant diameter and height were significantly higher following HP application. This experiment demonstrates the efficacy of HP that can cater the plant requirements at the initial growth stages which are crucial for their successful establishment in tropic urban degraded soils.     

Influence of Liming on Soil Chemical Properties and Plant Growth of Pineapple (Ananas Comusus L.Merr.) On Red Acid Soil,Lampung, Indonesia

ABSTRACT

Red acid soil is generally distributed in humid tropical areas under high rainfall. The main constraint is usually the extremely low pH of the soil due to the very intensive leaching of the bases from the soil. At the same time, however, the soluble micro elements, such as iron, are high. This can cause plant toxicity. The liming of acidic soils is normally performed to reduce the iron toxicity as the first step toward providing a balanced nutrition for cultivated plants. The objective of this study is to determine the effects of liming on the soil pH, on the decrease of iron in the soil and on the growth of the pineapple. The research was done in the Greenhouse of the Research and Development Department, PT Great Giant Pineapple, Lampung, Indonesia, from November 2015 to April 2016. The design of the experiment was arranged as a completely randomized design with seven treatments and three replications, consisting of: No dolomite (D0), dolomite 1 t ha^?1 (D1), dolomite 2 t ha^?1 (D2), dolomite 3 t ha^?1 (D3), dolomite 4 t ha^?1 (D4), dolomite 5 t ha^?1 (D5) with added Fe-EDTA and for the control treatment, no dolomite and no Fe-EDTA (C0). The results showed that an increase in the dolomite dose can increase the pH, potassium (K), calcium (Ca) and magnesium (Mg) in the soil and can decrease the iron (Fe) in the soil significantly. Increasing the pH, K, Ca and Mg and decreasing the Fe in the soil were seen to influence the growth of the pineapple. In particular, the leaf area of the pineapple plant increased considerably. The other parameters also increased, but not significantly.     

Influence of Azolla (Azolla microphylla Kaulf.) compost on biogenic gas production,inorganic nitrogen and growth of upland kangkong (Ipomoea aquatica Forsk.) in a silt loam soil

Azolla microphylla Kaulf. (Azolla) biomass was composted to create a high nitrogen (N) organic matter amendment (Azolla compost). We examined the effect of this Azolla compost on carbon (C) and N mineralization and the production of biogenic gases, nitrous oxide (N₂O) and carbon dioxide (CO₂), in a soil incubation experiment. A pot experiment with upland kangkong (Ipomoea aquatica Forsk.) examined plant growth in silt loam soil treated with three levels of Azolla compost. The results showed that N₂O production from soil increased with urea amendment, but not with Azolla compost treatments. The Azolla-amended soil showed enhanced CO₂ production throughout the 4-week incubation. The Azolla-treated soils showed a 98% lower global warming potential compared to urea treatment over the 4-week incubation. However, Azolla-amended soil had higher nitrate (NO₃^–) levels compared to urea-fertilized soil at 1 week of incubation, and these were maintained until the fourth week. Soils amended with Azolla compost showed lower ammonium nitrogen (NH₄-N) levels than those in the urea-fertilized soils. The height and dry weight of upland kangkong fertilized with Azolla compost were similar to plants receiving urea fertilization. Therefore, the use of Azolla compost as a substitute for urea fertilizer would be beneficial for reducing the production of N₂O while maintaining plant growth.     

生物质炭对砖红壤性质与养分及硝化作用的影响

土壤性质制约着作为氮转化重要环节的硝化作用,生物质炭显著影响与硝化作用密切相关的土壤性质,可能对硝化作用产生影响。本文利用培养试验研究生物质炭对砖红壤性质及硝化作用的影响。试验设生物质炭+淹水、生物质炭+75%田间持水量、空白+淹水及空白+75%田间持水量4个处理。研究表明,生物质炭能显著提高土壤pH值和有机碳含量以及N、P、K养分元素的有效性。水分条件差异影响生物质炭的作用效果,淹水更利于提高砖红壤的pH值,但显著降低了磷的有效性。添加生物质炭后,土壤硝化作用进程加速,硝化彻底。在利用生物质炭改良砖红壤时,应根据土壤改良目的调整土壤水分,以防硝态氮淋失风险和氨挥发的可能。     

Effects of Plant Growth Promoting Rhizobacteria on Yield and Some Fruit Properties of Strawberry

Effects of plant growth promoting rhizobacteria (PGPR) [(Pseudomonas BA-8 (biological control agent), Bacillus OSU-142 (N₂-fixing), and Bacillus M-3 (N₂-fixing and phosphate solubilizing)] on yield and some fruit properties of strawberry cultivar ‘Selva’ in the province of Erzurum, Turkey in 2002–2003. Foliar + root application of PGPR strains significantly increased yield per plant as compared with the control. Root application of PGPR strains significantly increased total soluble solids, total sugar and reduced sugar, but decreased titratable acidity. It was also determined that bacteria applications have no important effect on the average fruit weight and pH. The results of this study suggested that Pseudomonas BA-8, Bacillus OSU-142 and Bacillus M-3 have potential for increasing yield in strawberry plant.     

生物炭施用量对土壤改良及烤烟生长的影响

为了改良土壤容重,筛选优化生物炭施用量,以湖南烟区主要栽培品种云烟87为试验材料,通过大田试验研究3种不同的生物炭用量对田间土壤改良及烤烟生长的影响。结果表明,施用生物炭能够显著降低土壤容重,提高田间持水量,土壤容重与施炭量呈负相关,田间持水量与施炭量呈正相关。生物炭用量在3 750kg·hm~(-2)水平下,烤烟的植物学性状优良,干物质积累量大,烟株根系发达,根冠比协调,且氮肥利用率显著提高,但随着施炭量继续升高,烤烟的生长发育又相对受到抑制。综上所述,生物炭施用量为3 750kg·hm~(-2)较为适宜。本研究结果对利用生物炭改良植烟土壤,促进优质烟叶生产有一定的理论指导意义。