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以黄芩单细胞克隆愈伤组织为试材,采用聚丙烯酰胺凝胶电泳(PAGE)法,通过分析酯酶同工酶(EST)和过氧化物同工酶(POD)的酶带,研究黄芩单细胞克隆愈伤组织与亲本愈伤组织的遗传特性与品系差异。结果表明:黄芩不同品系单细胞克隆愈伤组织EST谱带间具3个带区特征,最多谱带数品系为4条,最少谱带数品系为3条,其中1号、2号、3号谱带为黄芩单细胞克隆愈伤组织的特征谱带;POD谱带间具4个带区特征,其中1号、3号和7号酶带为特征谱带,EST和POD谱带的有无、宽窄、颜色深浅,相对迁移率大小均不尽相同。黄芩单细胞克隆愈伤组织与亲本细胞愈伤组织既具有亲缘特征又具有品系差异,以期为单细胞克隆品系的遗传特性和品系鉴别提供理论参考。 相似文献
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食用菌过氧化物酶同工酶不同染色法效果比较 总被引:6,自引:0,他引:6
过氧化物酶同工酶(POD)是真菌遗传分析中的一个常用生化指标,在其电泳分析中染色操作是一个重要技术环节。本研究以香菇、美味侧耳为材料,比较了不同POD染色方法的染色效果。NH_3Cl-联苯胺法效果最佳,醋酸一联苯胺法次之,AC-联苯胺及愈创木酚-联苯胺法效果较差。对影响染色效果的原因进行了分析。 相似文献
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五种牛肝菌酯酶和过氧化物酶的同工酶研究 总被引:21,自引:2,他引:21
本文研究了滇产牛肝菌属的5个常见种的酯酶(EST)和过氧化物酶(POD)的同工酶。结果表明,(1)不同种牛肝菌子实体的EST和POD同工酶谱之间既有某些共同的特征,又各自具有本物种的特有特征;(2)5种牛肝菌子实体的EST和POD同工酶谱比较稳定,是一项稳定的分子水平的重要指标,完全可以作为鉴定牛肝菌属种类的重要依据。 相似文献
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罗布麻种质的过氧化物酶及酯酶同工酶研究 总被引:1,自引:0,他引:1
采用聚丙烯酰胺凝胶垂直板电泳技术,对不同地区罗布麻过氧化物酶和酯酶同工酶酶谱进行分析。结果表明:过氧化物酶具有2~5条不等的酶带,Rf值在0.547~0.882之间;酯酶具有3~5条不等的酶带,Rf值范围在0.1769~0.5918之间;过氧化物酶和酯酶的酶带数、酶带迁移率和酶活性可反映不同地区罗布麻种群间和种群内存在很大的遗传差异;DPS聚类分析结果表明:新疆红麻与内蒙红麻亲缘关系最近,宁夏红麻与河北红麻亲缘关系较近,青海白麻与内蒙古、新疆、宁夏和河北地区的亲缘关系最远。 相似文献
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通过野外观察、人工辅助授粉和室内测定等方式,对黄芩(Scutellaria baicalensis Georgi.)传粉生物学特性进行了研究。结果表明:黄芩花紫色、紫红色或蓝色,花冠口部直径0.5 ~ 1.2 cm,花冠筒长2.3 ~ 3.4 cm,花柱长2.3 ~ 3.5 cm,后对花药长0.1 ~ 0.15 cm,前对花药长0.18 ~ 0.3 cm,柱头及雄蕊不伸出花冠,不接触;花粉生活力在开花当天最强,花药刚开裂时有生活力花粉为76.94%,3 d后为54.06%;花粉/胚珠(P/O)为635 ~ 7 220,自然状态下结实率为56.74%。黄芩为异花虫媒授粉植物,传粉昆虫主要是沙漠条蜂(Anthophora deserticola Morawitz)、红光雄蜂[Bombus(Bombus)ignitus Smith]、花条蜂(Anthophora florae Smith)和富丽熊蜂[Bombus(Thoracobombus)opulentus Smith]等。 相似文献
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结球白菜同核异质雄性不育系同工酶研究 总被引:18,自引:1,他引:17
采用聚丙烯酰胺凝胶电泳法,研究了具有甘蓝型油菜胞质、萝卜胞质的结球白菜同核异质雄性不育系-Polcms3411-7,Ogucms3411-7及其保持系3411-7不同发育时期,不同器官过氧化物酶同工酶谱的差异。结果表明,不育系与保持系过化物酶同工酶谱的差异主要在花蕾中,表现为不育系酶带多而且活性强,其它器官差异较小;Polcms3411-7与Ogucms3411-7的区别在苗期、蕾期、花期及叶片、 相似文献
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AIM: To study the effects of flavonoids isolated from Scutellaria stem and leaf (SSF) on the expression of N-methyl-D-aspartate receptor (NMDAR) and vascular endothelial growth factor (VEGF) in chronic cerebral ischemia rats. METHODS: The model of chronic cerebral ischemia was established by bilateral carotid artery occlusion for 2 months in female SD rats. The effects of SSF on mRNA expression of NMDAR in hippocampus and VEGF in cerebral cortex were evaluated by the method of RT-PCR. RESULTS: Compared with the sham group, the expression of NMDAR1, NMDAR2A and NMDAR2B in hippocampus and VEGF in cerebral cortex were significantly increased (P<0.01). However, the cerebral ischemia rats daily and orally administered with SSF at doses of 17.5 mg·kg-1·d-1, 35 mg·kg-1·d-1 and 70 mg·kg-1·d-1 for 38 days appeared that the mRNA expression of NMDAR1, NMDAR2A and NMDAR2B in hippocampus was obviously reduced (P<0.05), and the mRNA content of VEGF in the cortex (P<0.05) was increased. CONCLUSION: SSF decreases the expression of NMDAR in hippocampus, increases the expression of VEGF in cerebral cortex of cerebral ischemia rats, suggesting that the neuroprotective effect of SSF may be exerted by influencing the production of NMDAR and VEGF in the brain. 相似文献
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葡萄叶片中POX及其同工酶与抗黑痘病的关系 总被引:5,自引:0,他引:5
选用对葡萄黑痘病抗性不同的3种类型(高抗、中抗、感病)葡萄,研究其正常功能叶、幼叶(感病前期、初期、后期)POX活性及其同工酶谱变化情况。结果表明,叶片POX活性与葡萄抗黑痘病呈正相关。高抗类型POX活性明显高于中抗和感病类型,正常功能叶POX活性高于幼叶;高抗类型与中抗类型POX活性在幼叶感病前后呈上升趋势,而感病类型则呈先升后降趋势。抗性强弱不同嵉睦嘈停校希赝っ赣邢灾钜臁1硐治呖估嘈兔复氖俊⒖矶燃把丈疃染哂诟胁±嘈停徊宦鄹胁±嘈汀⒖共±嘈停溆滓陡胁『竺复俊⑶慷染哂诟胁∏啊 相似文献
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以甜瓜鲁厚甜1号开花当天的果实为试材,分别测定蔗糖代谢相关酶—酸性转化酶(AI)、中性转化酶(NI)、蔗糖合成酶(SS)和蔗糖磷酸合成酶(SPS)的活性。对酶提取液中干扰测定结果的可溶性糖采取2种方法进行去除(1)直接用SephadexG-25柱离心去糖;(2)酶提取液先经过硫酸铵沉淀,去糖,重新溶解后再用SephadexG-25柱脱盐。结果显示,方法2处理后AI和NI、SPS和SS的活性均高于方法1。这表明在研究甜瓜果实蔗糖代谢相关酶活性时,采用方法2处理酶提取液效果更好。 相似文献
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AIM: To investigate the effect of flavonoids from stem and leaf of Scutellaria baicalonsis Georgi (SSF) on paired helical filament (PHF) abnormality and the regulatory mechanism of protein phosphatase (PP) in rats' brain induced by okadaic acid (OA). METHODS: Male Sprague-Dawley (SD) rats were microinjected with OA (200 ng/kg) by the lateral ventricle to establish a memory impairment model. Morris water maze was used to screen the memory impairment model. The successful model rats were continuous intragastric infusion (ig) SSF for 36 days. The relative protein expression of PHF, PP1, PP2A-Cα, PP2A-Cβ, PP2CA and PP2CB in the rat cerebral cortex and hippocampus were detected by Western blot. GinKgo biloba leaf flavonoids (GLF) were used as positive control drug. RESULTS: Compared with the sham-operated rats, the relative protein expression of PHF in the cerebral cortex and hippocampus and PP1 in cortex of model rats were significantly increased (P<0.01), and the protein expression of PP2A-Cα, PP2A-Cβ in the cerebral cortex and hippocampus and PP2CB in the hippocampus were decreased (P<0.05), while the relative protein expression of PP2CA and PP2CB in the cortex were significantly increased (P<0.01). SSF reversed the abnormality in the protein expression of PHF, PP2A-Cα and PP2A-Cβ in rat cortex and hippocampus and PP1 in rat cortex induced by OA (P<0.01), which had no significant effect on the relative protein expression of PP2CA and PP2CB. GLF also showed similar results to SSF. CONCLUSION: SSF significantly reduces the abnormal formation of PHF in rats' brain induced by OA, which may be related to the regulation of PP1, PP2A-Cα and PP2A-Cβ expression, but not with PP2CA and PP2CB expression. 相似文献