Efficacy of PGF(2α) on pre-ovulatory follicle and corpus luteum blood flow

The aim of this study was to evaluate the effect of cloprostenol administration on the blood flow of pre-ovulatory follicle (PF) and corpus luteum (CL), progesterone secretion and pregnancy outcome in buffaloes subjected to AI. The trial was performed on 75 Italian buffaloes at 182 ± 8 days in milk. Synchronized animals were randomly divided into two groups on the day of oestrus: Group T (n = 37) received a 0.524 mg intramuscular injection of cloprostenol and Group C (n = 38) received saline. Ultrasound examinations of the ovaries were performed 5 h after AI on the PF and 10 and 20 days after AI on the CL. Resistive (RI) and pulsatily index (PI) were calculated by colour-Doppler mode in each examination. Blood samples were collected on days 10, 20 and 25 after AI for progesterone assay and 25 days after AI, ultrasonography was performed to assess pregnancy, which was confirmed on day 45. Subjects pregnant on day 25 but not on day 45 were considered to have undergone late embryonic mortality (LEM). Statistical analysis was performed by anova. No differences were found in PF dimensions, CL size and blood flow on day 10 and 20 after AI between treated and control groups. Pre-ovulatory follicle area was higher in buffaloes that resulted pregnant on day 25 after AI compared to those that were non-pregnant (2.13 vs 1.66 cm in pregnant and non-pregnant buffaloes, respectively), while non-pregnant buffaloes showed higher values of RI (0.49 vs 0.30; p < 0.05) and PI (1.0 vs 0.37; p = 0.07) compared to pregnant subjects. Treatment by cloprostenol did not influence pregnancy rate both on day 25 (31/75; 41.3%) and 45 (27/75; 36.0%), progesterone levels and incidence of LEM (4/31; 12.9%). In conclusion, cloprostenol administration at the time of AI does not seem to affect PF and CL blood flow.     

乏情黄牛应用前列腺素(PGF2α)促情效果试验

<正>利用某些激素制剂处理,人为的控制并调整母畜发情周期,使之同期化的方法称为同期发情。此方法可使母畜在预定的时间内集中发情,以便有计划的合理组织配种,有利于推广人工授精     

用PGF2α处理提高奶牛产后繁殖力

一、前言从外周血中PGFM水平的测定结果可知,分娩前黄体消退并伴有PGF_2α的暂时性升高,黄体的最终消退是PGF_2α溶体黄作用引起的;产后1～4天外周血中PGFM增加,15天左右恢复到基础水平。试验证实,产后早期PGFM主要来源于子宫。因为切除子宫后,不出现产后正常的PGFM水平的上升。子宫动、静脉血中PGFM水平的差异,也表明子宫是PGF_2α的合成、代谢部位。子宫复旧是产后卵巢恢复周期性活动的先决条件,产后子宫复旧时间过长与产后早期内源性PGF_2α的分泌不足有关。PGF_2α能收缩子宫平滑肌,增加子宫紧张性。产后PGFM高     

利用前列腺素(PGF2a)使牛同步发情(摘译)

连续两天采用非外科手术对发情周期第四天以后的母牛,用输精器直接输入靠近有黄体的卵巢一侧的子宫角中前列腺素(PGF2a)每天0.5mg,形成一个极为有效的引诱母牛发情的方法,并且因此使母牛同时发情。     

Fertility of Bunaji (Zebu) Cows after Treatment with PRID with or without PGF2α

A study was undertaken to determine the oestrus response and fertility rates of zebu cows treated with PRID alone or in combination with PGF2alpha. A total of 184 non-suckled cycling Bunaji cows were allotted randomly to four treatment groups of 46 animals per group as follows: group 1 (PRID-12), PRID was inserted for 12 days; group 2 (PRID+7+PGF2alpha-6, PRID was inserted for 7 days and PGF2alpha was administered intramuscularly 1 day prior to PRID withdrawal; group 3 (PRID-7+PGF2alpha-7, PRID was inserted for 7 days and PGF2alpha was administered intramuscularly on the day of PRID withdrawal; group 4 (2 x PGF2alpha-13, two intramuscular injections of PGF2alpha 13 days apart. At the end of each treatment period, the cows were observed for 7 days for behavioural oestrus and were inseminated 12 h following detection of oestrus. Pregnancy was diagnosed by rectal palpation 30-40 days post-insemination. The respective oestrus response rates were 78.3%, 76.1%, 87.0% and 89.1% for groups 1-4. While the corresponding pregnancy rates were 39.1%, 41.3%, 52.2% and 52.2%, the conception rates were 50.0%, 54.3%, 60.3% and 58.6% for groups 1-4. Although individual variations in progesterone levels were observed, the progesterone profiles were generally typical and normal. The results of the study have confirmed the effectiveness of the four regimes in synchronizing and controlling oestrus and ovulation in Bunaji cows. However, groups 3 and 4 showed some superiority over the other treatments. The results of this study provide feasible options from which clinicians involved in intensive breeding programmes and herd health fertility programmes can choose.     

湖羊黄体期注射PGF2α对黄体组织形态、PGF2α受体和程序性坏死相关基因表达的影响

旨在研究不同黄体期注射前列腺激素(PGF2α)对湖羊育成母羊黄体组织形态、PGF2α受体及程序性坏死通路基因表达的影响。本研究选择健康、体况良好、体重相近经同期发情处理的母羊48只，随机均分为6组，分别为黄体前期试验组和对照组、中期试验组和对照组和末期试验组和对照组母羊，试验组在发情周期第6天(黄体前期)、11天(黄体中期)、16天(黄体末期)注射1 mL PGF2α(0.1 mg),对照组在相同时间注射1 mL生理盐水，每次注射后3 h采集黄体组织，用于基因表达检测及HE组织染色检测黄体组织形态变化。结果表明，注射PGF2α后试验羊黄体组织形态发生了变化，出现炎症细胞浸润与细胞凋亡，其中黄体中期退化程度最高。未注射PGF2α的母羊，黄体前期、中期FPA mRNA表达水平显著高于黄体末期(P<0.05);黄体前期FPB表达水平显著高于黄体末期(P<0.05),与黄体中期无显著差异(P>0.05);黄体中期TNFR1表达水平显著高于黄体前期和末期(P<0.05);黄体中期RIPK3表达水平显著高于黄体末期(P<0.05),黄体中、末期与黄体前期差异均不显著(...     

PGF2α对奶牛产后胎衣排出效果临床试验

将自然分娩后于８小时内尚未排出胎衣的６８头奶牛平均分为两个组：治疗组肌肉注射２５毫克前列腺素Ｆ＿（２α）（合成ＰＧＦ＿（２α））；对照组肌肉注射５毫升生理盐水。经ＰＧＦ＿（２α）所治疗的３４头牛中有２７头（７９．４％）和对照组的３４头牛中仅有１８头（５２．７％），于１２小时内胎衣完全排出，差异显著（Ｐ＜０．０５）。     

应用PGF2α类似物对马分娩的诱发

角田等(1978)和三宅等(1982)报告了农用马的难产死亡和生后死亡的发生率约占全部分娩数的1/10。角田等(1978)也报道,马的分娩约有83.4％发生在下午6点到第二天早晨6点之间。因此,在白天如果能够诱发分娩,则可对异常分娩进行迅速处置,而减少产驹的损失。关于马的分娩诱发有很多报告,如Hillman(1975)及Rossdale和Jeffcott(1975)单用或与催产素及已烯雌酚并用,又Rossdale投给地噻咪松     

Luteolysis and associated interrelationships among circulating PGF2α, progesterone, LH, and estradiol in mares

The changing concentrations and temporal relationships among a PGF2α metabolite (PGFM), progesterone (P₄), LH, and estradiol-17β (E₂) before, during, and after luteolysis were studied in 10 mares. Blood samples were collected every hour for ≥4 d beginning on day 12 after ovulation. The luteolytic period extended from a decrease in P₄ at a common transitional hour (Hour 0) at the end of preluteolysis and beginning of luteolysis to a defined ending when P₄ reached 1 ng/mL. The length of luteolysis was 22.9 ± 0.9 h, contrasting with 2 d in published P₄ profiles from sampling every 6 to 24 h. In mares with complete data for Hours −40 to −2 (n = 6), PGFM concentrations remained below assay sensitivity (n = 2) or two or three small pulses (peak, 29 ± 4 pg/mL) occurred. During luteolysis, the pulses became more prominent (peak, 193 ± 36 pg/mL). Rhythmicity of PGFM pulses was not detected by a pulsatility program during preluteolysis but was detected in seven of nine mares during luteolysis and postluteolysis combined. The nadir-to-nadir interval for LH pulses and the peak-to-peak interval between adjacent pulses were longer (P < 0.05) during preluteolysis than during luteolysis (nadir to nadir, 5.2 ± 0.3 h vs 3.6 ± 0.4 h; peak to peak, 9.4 ± 1.0 h vs 4.7 ± 0.5 h). Unlike reported findings in cattle, concentrations of P₄ decreased linearly within the hours of each PGFM pulse during luteolysis, and a positive effect of an LH pulse on P₄ and E₂ concentration was not detected. The reported balancing of P₄ concentrations between a negative effect of PGF2α and a positive effect of LH in heifers was not detected in mares.     

Acute changes in the concentrations of prostaglandin F2α (PGF) and cortisol in uterine and ovarian venous blood during PGF-induced luteolysis in cows

Prostaglandin F2α (PGF) is considered to be the main luteolysin in cattle. We have previously demonstrated that cortisol (Cr) suppresses PGF production in non-pregnant bovine endometrium. This study was carried out to test whether exogenous PGF increases ovarian and/or uterine PGF production and to determine the temporal relationship between PGF and Cr in ovarian and uterine circulations during PGF-induced luteolysis in cows. Catheters were inserted into the ovarian vein (OV), uterine vein (UV) and jugular vein (JV) of 10 cows on Day 9 of the oestrous cycle (Ovulation = Day 0) for frequent blood collection. On Day 10, the cows were divided randomly into two groups and treated with a luteolytic dose of a PGF analogue (cloprostenol) or saline solution. Blood samples were collected at -0.25, 0, 0.25, 0.5, 1 and 2 h and then at 2-h intervals until 12 h after treatment (0 h). The basal concentrations of PGF and Cr in OV and UV plasma were not significantly different. Injection of a PGF analogue induced more than twofold increases in the levels of PGF between 0.25 and 1 h in UV plasma, but not in OV plasma. PGF increased (p < 0.05) the concentrations of Cr in OV, UV and JV plasma between 0.5 and 1 h. The Cr levels in OV, UV and JV plasma were similar. The PGF levels in UV plasma decreased after Cr reached its highest levels. The overall results suggest that the uterus rather than the ovary increases PGF production in response to PGF injection. Based on the temporal changes of PGF and Cr in the ovarian and uterine circulations, Cr may act to reduce uterine PGF production in non-pregnant cows in vivo.     

GnRH、PGF2α诱导同期发情效果的比较

在奶牛进行定时人工授精的过程中 ,需要较多数量同期发情的母牛。同期发情处理是指用某些激素控制并调整一群母畜发情的过程 ,使之在预定的时间内发情 ,以便进行规模化人工授精和胚胎移植。在应用药物进行同期发情处理时 ,有两种类型的方法 ,一类是延长黄体期的孕酮法 ;另一类是缩短黄体期的前列腺素法 (ＰＧ法 )。虽然这两种方法在实际生产中已有应用 ,但同期发情的效果都不尽人意。近年来 ,促性腺激素释放激素 (ＧｎＲＨ)及其类似物对家畜的同期发情作用已取得令人满意的效果 ,并具有操作方便、发情率高等特点。本文就ＧｎＲＨ类似物和Ｐ…     

GnRH、PGF2α诱导同期发情效果的比较

153头健康经产的中国荷斯坦奶牛分为3个组,用3种不同的方法进行同期发情处理.第1组注射1次氯前列烯醇;第2组注射2次氯前列烯醇;第3组注射促排3号--氯前列烯醇--促排3号.结果表明第1组、第2组和第3组经不同的处理后,发情率分别为32.65%(16/49)、45.59%(31/68)和91.30%(42/46),第3组的发情率分别显著高于其它2个组(P＜0.05).结果提示促排3号--氯前列烯醇--促排3号是较适合中国荷斯坦奶牛的同期发情方案.     

GnRH结合PGF2α法处理水牛同期发情试验

试验应用GnRH结合PGF2α法处理水牛进行同期发情试验的研究,应用于水牛胚胎移植的实践中,并取得较好的效果.为建立最佳的同期发情方法和为胚胎移植的产业化奠定理论和实践基础,现将试验报道如下.     

GnRH、PGF2α诱导同期发情效果的比较

本文就同期发情的不同方法进行了比较 ,以寻找适合本地环境的同期发情方案。 15 3头健康经产的中国荷斯坦牛分为 3个组 ,用 3种不同的方法进行同期发情处理。第 1组 :注射 1次氯前列烯醇 ;第 2组 :注射 2次氯前列烯醇 ;第 3组 :注射促排 3号 -氯前列烯醇 -促排 3号。结果表明 :第 1组、第 2组和第 3组发情率分别为 32 6 5 % (16 4 9) ,4 5 5 9% (31 6 8)和 91 30 %(42 4 6 ) ,第 3组的发情率分别显著高于其它两个组 (P <0 0 5 )。结果提示促排 3号 -氯前列烯醇-促排 3号可能是较适合中国荷斯坦牛的同期发情方案。     

GnRH、PGF2α诱导同期发情效果的比较

153头健康经产中国荷斯坦牛分为3组，用3种方法进行同期发情处理。第1组注射1次氯前列烯醇；第2组注射2次氯前列烯醇；第3组注射促排3号－氯前例烯醇－促排3号。结果表明：第1，2，3组发情率分别为32．65％（16／49），45．59％（31／68）和91．30％（42／46），第3组发情率显著高于其它两组（P＜0．05）。     

氯前列烯醇(PGF2α)不同处理方法对安化本地水牛同期发情效果的研究

为提高母水牛情期受胎率,促进安化县水牛品种改良工作的开展,文章就氯前列烯醇不同处理方式对本地水牛同期发情效果进行了比较研究试验。结果表明:灌注一次PG、注射一次PG、灌注两次PG和注射两次PG的同期发情发情率分别为55.56%、63.64%、60.00%和76.92%,总体受胎率分别为22.22%、27.27%、33.33%和38.46%;一次PG处理组同期发情率为60%,二次PG同期发情率为69.56%,总体受胎率分别为25.00%和34.78%;灌注组的同期发情率同期发情发情率为57.89%,显著高于肌注PG组的57.89%,总体受胎率分别为33.33%和26.32%。利用PG进行滨湖水牛同期发情处理时,子宫内两次灌注法能得到较好的发情率和受胎率。