Effect of dietary selenium and vitamin E on the ultrastructure and ATP concentration of boar spermatozoa, and the efficacy of added sodium selenite in extended semen on sperm motility

Three experiments evaluated the effects of dietary Se and vitamin E on the ultrastructure of spermatozoa, ATP concentration of spermatozoa, and the effects of adding sodium selenite to semen extenders on subsequent sperm motility. The experiment was a 2 x 2 arrangement of treatments in a randomized complete block design. A total of 10 mature boars were fed from weaning to 18 mo of age diets fortified with two levels of supplemental Se (0 or .5 ppm) or vitamin E (0 or 220 IU/kg diet). The nonfortified diets contained .06 ppm Se and 4.4 IU vitamin E/kg. In Exp. 1, the spermatozoa from all boars were examined by electron microscopy. Vitamin E had no effect on structural abnormalities in the spermatozoa. When the low-Se diet was fed the acrosome or nuclei of the spermatozoa was unaffected, but the mitochondria in the tail midpiece were more oval with wider gaps between organelles. The plasma membrane connection to the tail midpiece was not tightly bound as when boars were fed Se. Immature spermatozoa with cytoplasmic droplets were more numerous when boars were fed the low-Se diet, but the occurrence of midpiece abnormalities occurred in boars fed diets with or without Se or vitamin E. Our results suggest that Se may enhance spermatozoa maturation in the epididymis and may reduce the number of sperm with cytoplasmic droplets. In Exp. 2, the concentration of ATP in the spermatozoa was evaluated in the semen of all treatment boars. When the low-Se diet was fed, ATP concentration was lower (P < .01), whereas vitamin E had no effect on ATP concentration. Experiment 3 investigated the effect of diluting boar semen with a semen extender with sodium selenite added at 0, .3, .6, or .9 ppm Se. Three ejaculates from each boar were used to evaluate these effects on sperm motility to 48 h after dilution. Sperm motility declined (P < .01) when Se was added to the extender, and this decline was exacerbated as the concentration of added Se increased (P < .01). The added Se was demonstrated to be tightly adhered to the spermatozoa. Overall, these results suggest that low Se-diets fed to boars resulted in abnormal spermatozoal mitochondria, a lower ATP concentration in the spermatozoa, and a loose apposition of the plasma membrane to the helical coil of the tail midpiece, but no effect from inadequate vitamin E was demonstrated. Adding sodium selenite to the semen extender reduced sperm cell motility.     

Effect of vitamin supplements on some aspects of performance, vitamin status, and semen quality in boars

The aim of the present study was to determine the effects of dietary supplements of vitamins on vitamin status, libido, and semen characteristics in young boars under normal and intensive semen collection. Sixty Landrace, Yorkshire, and Duroc boars were allocated randomly from 6 to 10 mo of age to one of the following diets: 1) basal diet (industry level) for minerals and vitamins (Control, n = 15); 2) basal diet supplemented with vitamin C (ASC, n = 15); 3) basal diet supplemented with fat-soluble vitamins (FSV, n = 15); and 4) basal diet supplemented with water-soluble vitamins (WSV, n = 15). After puberty (approximately 12 mo of age), semen was collected at a regular frequency (three times every 2 wk) for 5 wk. Thereafter, all boars were intensively collected (daily during 2 wk). A recovery period (semen collection three times every 2 wk) followed and lasted for 10 wk. Sperm quality (percentage of motile cells and percentage of morphologically normal cells) and quantity (sperm concentration, semen volume, and total sperm number) were recorded as well as direct and hormone related measurements of boar libido. Blood and seminal plasma samples were taken to monitor vitamin status. High concentrations of B6 (P < 0.05) and folic acid (P < 0.05) were observed in the blood plasma of WSV boars, whereas greater concentrations of vitamin E (P < 0.01) were obtained in FSV boars. In the seminal plasma, folic acid concentrations tended to be greater in WSV boars (P < 0.08). During the intensive collection period, there was a tendency (P < 0.06) for semen production to be greater in WSV boars, the effect being less pronounced (P < 0.10) in FSV boars. During the recovery period, the percentage of motile sperm cells was greater in WSV boars (P < 0.03) and, to a lesser extent, in FSV boars (P < 0.10) compared with Control boars. Sperm morphology and libido were not affected by treatments. These results indicate that the transfer of vitamins from blood to seminal plasma is limited and the dietary supplements of water-soluble and fat-soluble vitamins may increase semen production during intensive semen collection.     

Effects of dietary supplementation with an organic source of selenium on characteristics of semen quality and in vitro fertility in boars

Semen characteristics in boars fed organic or inorganic sources of Se were assessed in 3 experiments. Crossbred boars were randomly assigned at weaning to 1 of 3 dietary treatments: I) basal diets with no supplemental Se (control), II) basal diets with 0.3 mg/kg of supplemental Se from an organic source (Sel-Plex, Alltech Inc., Nicholasville, KY), and III) basal diets supplemented with 0.3 mg/kg of supplemental Se from sodium selenite (Premium Selenium 270, North American Nutrition Co. Inc., Lewisburg, OH). For Exp. 1, semen was collected from boars (n = 10/dietary treatment) on 5 consecutive days at 15 mo of age. Effects of treatment × day were detected for the proportions of progressively motile (P = 0.02) and rapidly moving (P = 0.03) spermatozoa, and measures of sperm velocity, including path velocity of the smoothed cell path (P = 0.05) and average velocity measured in a straight line from the beginning to the end of the track (P = 0.05). Negative effects of day of semen collection on sperm motility were least pronounced in boars fed Sel-Plex. Experiment 2 was conducted when boars were 17 mo of age, and semen was collected (n = 10 boars/dietary treatment), diluted in commercially available extenders, and stored at 18°C for 9 d. Effects of treatment × day were detected for percentages of motile (P = 0.01) and static (P = 0.01) spermatozoa, amplitude of lateral head displacement (P = 0.02), frequency with which the sperm track crossed the sperm path (P = 0.04), straightness (P = 0.01), and average size of all sperm heads (P = 0.03). In general, sperm cells from boars fed Sel-Plex were better able to maintain motility during liquid storage compared with boars fed sodium selenite. For Exp. 3, semen was collected from boars (n = 6/dietary treatment) at 23 mo of age, and spermatozoa were evaluated at d 1 and 8 after semen collection using in vitro fertilization procedures. There was a tendency for an effect (P = 0.11) of dietary treatment on fertilization rate with Sel-Plex-fed boars having the greatest value (70.7%). The results of this study suggest that there are positive effects of dietary supplementation with Sel-Plex on boar semen characteristics and that organic Se supplementation may help ameliorate the negative effects of semen storage on characteristics of sperm motility.     

Active immunization of prepubertal boars against testosterone: testicular and endocrine responses at 14 months of age

Thirteen crossbred boars were immunized at 1 mo of age against either testosterone-3-oxime-equine serum albumin (treated boars) or equine serum albumin (control boars) to test the hypothesis that active immunization against testosterone stimulates testicular growth and development in the prepubertal boar. All boars were injected with the appropriate antigen at 2, 3, 4, 5 and 6 mo of age and were slaughtered at 14 mo of age. Active immunization against testosterone resulted in an increase (P less than .05) in tritiated-testosterone binding by plasma within 60 d after the primary immunization; the degree of binding decreased by 6 mo but remained elevated (P less than .05) relative to controls through 12 mo of age. There was no effect of treatment on body weights through 12 mo of age. Concentrations of testosterone in plasma were higher (P less than .05) in testosterone-immunized boars than in controls; this increase was likely due to antibody binding rather than increased testosterone secretion because (1) concentrations of androgen in testicular parenchyma at slaughter were not altered by treatment and (2) plasma concentrations of estrogens were generally not affected by treatment. Concentrations of luteinizing hormone (LH) and follicle stimulating hormone (FSH) were markedly suppressed in testosterone-immunized boars during the time when concentrations of these gonadotropins were high in control boars (greater than 3 mo of age). In spite of suppression of average LH and FSH concentrations, testicular weights, daily sperm production rates and seminal characteristics were similar for the two groups of boars at slaughter. (ABSTRACT TRUNCATED AT 250 WORDS)     

OC4Influence of Seminal Plasma Added to Highly Diluted Semen on Sperm Motility of Young Boar Feed with Selenium and Vitamin E

High dilution rates have been documented as detrimental for boar spermatozoa, shortening their lifespan (Centurion et al. 2003, Biol Reprod 69: 640–646). Addition of seminal plasma (SP) to semen extenders, or selenium (Se) and vitamin E (VE) in diet of boars could increase motility of highly diluted spermatozoa (HDS). The aim of this work was to evaluate the effect of seminal plasma on sperm motility of HDS from boars feed with Se and VE. Sixteen 12 month-old boars were designed to one of four dietary treatments: (i) control, Se 0 ppm–VE 0 IU/kg; (ii) Se 0–VE 250; (iii) Se 0.5–VE 250 and (iv) Se 0.5–VE 0. Boars were treated for 8 weeks before semen collection. Sperm rich fractions from each boar were diluted to 5 × 10⁶ sperm/ml in PBS medium and incubated at 37°C with or without 10% SP. The measurements were done at 0, 2 or 5 h. Data were analyzed as a mixed model for a factorial design [2 (Se) × 2 (VE) × 2 (SP) × 3 (h)]. Percentage of sperm motility (PSM) increases significantly (p < 0.001) with addition of Se (81.3 ± 1.52), VE (81.0 ± 1.62) and SP (81.5 ± 1.57) vs control (73.4 ± 1.61). There was significant interaction Se × VE (p < 0.001) and Se × VE × SP (p < 0.05) in PSM. However, PSM was affected significantly by time (0 h 83.4 ± 1.92; 2 h 80.7 ± 1.92 and 5 h 67.9 ± 1.92; p < 0.001). There was significant interaction SP × Time (p < 0.05) in PSM. These results indicate that Se, VE and SP improve seminal viability. Addition of 10% of SP maintains PSM at least during 5 hours.     

The influence of duration of photoperiod and hemicastration on growth and testicular and endocrine functions of boars

Yorkshire boars were used to evaluate the influence of duration of photoperiod and hemicastration on growth and testicular and endocrine functions. At 10 wk of age, 5 hemicastrate (HC) and 5 intact (I) boars were assigned to either 8 or 16 hr of light daily until 6 mo of age. Body weights were recorded biweekly throughout the experiment. Venous cannulae were placed in all boars at 6 mo of age, and serum was collected at 30 min intervals from 0800 to 2000 hr. Gonadotropin releasing hormone (GnRH) was infused at 2000 hr (50 micrograms) and at 2030 hr (250 micrograms), and samples of serum were collected until 2400 hr. The following day, all boars were castrated, and the weights and sperm content of the testes and epididymides were determined. At castration, all pigs were given implants containing testosterone. Two weeks later, pigs were again canulated, and serum was obtained at 15 min intervals for 2 hr. Growth of boars was not significantly affected by duration of photoperiod or number of testes. Duration of photoperiod did not affect weight or sperm content of testes or epididymides. Hemi-castrated boars had greater testicular (P less than .01) and capita-corpora (C-C) epididymal weights (P less than .05) and more testicular and C-C sperm (P less than .01) per testis. Neither average concentrations of luteinizing hormone (LH) nor number and amplitude of pulses of LH were affected by photoperiod treatment. However, HC boars had greater average concentrations of LH (P less than .05) than I boars (.71 +/- .05 vs .52 +/- .05 ng/ml). Hemicastrated boars in 16 hr light daily had greater concentrations of FSH in serum (P less than .05) than 8I, 8HC, and 16I boars. Intact and HC boars had similar concentrations of prolactin (PRL) and testosterone. Similarly, concentrations of PRL and testosterone were not affected by duration of photoperiod. Secretion of LH and testosterone after treatment with GnRH was not significantly affected by duration of photoperiod. In general, HC boars released more LH in response to GnRH treatment than I boars. Concentrations of LH were greater (P less than .05) in HC than I boars at .5, 1, 2, and 3 hr after GnRH and tended (P less than .10) to be elevated at 1.5, 2.5, 3.5 and 4 hr after GnRH. The FSH response to GnRH was greater (P less than .05) for 16HC than 8I, 8HC, or 16I boars.(ABSTRACT TRUNCATED AT 400 WORDS)     

Relationship of absolute numbers of Sertoli cells to testicular size and spermatogenesis in young beef bulls

Testes were obtained from 34 Hereford or Angus bulls at about 1.5 yr of age and were used to investigate the relationship between the absolute number of Sertoli cells vs testicular size and daily spermatozoal production (DSP). Quantitative determination of DSP was based upon enumeration of elongated spermatids in testicular homogenates. The ratio of step 8 spermatids to Sertoli cells (S:SC) was established by direct counts of these cells in each of 20 round stage VIII seminiferous tubular cross sections for each bull. The number of Sertoli cells per paired testes was calculated as (total spermatids divided by S:SC)/.394, where total spermatids equalled the number of homogenization-resistant spermatids. The factor of .394 adjusted for the fact that the latter cells are present for only 39.4% of the spermatogenic cycle. All data were subjected to simple linear and second-order regression analyses. A positive linear relationship (P less than .005) was found between testicular weight (Y, in grams) and the absolute number of Sertoli cells per paired testes (X, in billions), which was characterized by the equation Y = 315.2 + 10.74X and a coefficient of correlation (r) of .56 (P less than .01). A similar relationship was observed between DSP (Y, in billions) and Sertoli cell numbers (X, in billions). This was characterized by the equation Y = 1.36 + .222X (P less than .005) and a coefficient of correlation of .70 (P less than .01). Daily sperm production was unrelated to the S:SC ratio (P greater than .05).(ABSTRACT TRUNCATED AT 250 WORDS)     

Semen characteristics and libido in boars treated repeatedly with PGF2alpha

The objective was to determine the effects of repeated injections of PGF2alpha (Lutalyse; Pfizer) on semen and libido characteristics in terminal-line boars. Semen was collected once weekly from wk 0 to 15 and on four consecutive days during wk 16. Boars received an i.m. injection of 10 mg of PGF2alpha (n = 11) or 2 mL of vehicle (n = 11) immediately before entering the collection room. For the weekly collections, semen volume (220.3 +/- 3.2 mL; mean +/- SE), gel weight (38.7 +/- 0.7 g), total sperm cells (65.4 +/- 1.2 billion), motile sperm cells (67.4 +/- 0.6 %), and sperm velocity (125.9 +/- 1.2 microm/s), were affected by time (P < 0.01) but not by treatment (P > 0.10). Sperm concentration (0.31 +/- 0.01 billion/mL) was not affected (P > 0.10) by time or treatment. The percentage of morphologically normal sperm cells, assessed at wk 16, did not differ (P = 0.39) between groups (80.8 +/- 1.0). Libido was evaluated from wk 0 to 16. There were no effects of treatment or time (P > 0.10) on the period from injection to the start of ejaculation (225.6 +/- 9.1 s). Duration of ejaculation was affected by treatment (P < 0.01; 472.0 +/- 43.1 s and 280.4 +/- 43.1 s, for PGF2alpha-treated and control boars, respectively) and time (P < 0.01). During the intensive collection period (wk 16), semen volume (200.1 +/- 7.1 mL), gel weight (39.2 +/- 1.5 g), sperm concentration (0.19 +/- 0.01 billion/ mL), total sperm cells (39.5 +/- 3.6 billion), motile sperm cells (65.6 +/- 2.2%), and sperm velocity (117.8 +/- 3.7 microm/ s) were affected by time (P < 0.10) but not by treatment (P > 0.10). The period from injection to the start of ejaculation tended to decrease (by 44%) during the intensive collection period in PGF2alpha-treated boars, but not in controls (treatment x time, P = 0.07). Regardless, the period from injection to the start of ejaculation did not differ (P = 0.63) between groups on d 4 of the intensive collection period. Duration of ejaculation was affected by treatment (P < 0.01; 459.1 +/- 24.1 s for PGF22alpha-treated boars vs. 303.1 +/- 24.1 s for controls) but not by time (P > 0.10). Overall, there were no exceptional positive or negative effects of long-term treatment with PGF2alpha on semen characteristics and libido in boars.     

Infertile Boars with Knobbed and Immotile Short‐tail Sperm Defects in the Finnish Yorkshire Breed

In the period 1996–2006 two specific sperm defects, the knobbed acrosome (KA) defect and the immotile short‐tail sperm (ISTS) defect, showed a strong negative association with fertility in Finnish breeding boars. In this study, we examined the incidence of these two sperm defects in two pig breeds, their effects on fertility and their associations with sperm morphology and testicular histology. Semen samples from 2048 (1097 Yorkshire, 951 Landrace) boars were collected. None of the Landrace boars revealed either the KA defect or the ISTS defect. Of the Yorkshire boars, 0.8% were afflicted with the KA defect and 7.6% with the ISTS defect. Boars diagnosed with the ISTS defect produced no litters. Fertility data were available from two artificially inseminated (AI) boars and six farm breeding boars affected with the KA defect. Breeding boars with 45–81% knobbed spermatozoa (n = 6) did not produce any litters out of 71 sows bred. AI boars with 25–30% knobbed spermatozoa had a poor non‐return rate (on average 47% compared with 85% for normal control boars) and produced small litters, on average 2.5 piglets less than other boars of the same breed. Morphometry of testicular tissue and distribution of different cells in the seminiferous tubules were examined in nine boars. Boars with the KA defect had a smaller diameter of the seminiferous tubules (p < 0.05) and a lower number of Sertoli cells (p < 0.05) than controls. ISTS boars, in turn, had a significantly lower number of elongated spermatids (p < 0.05), and they also produced on average only 12% of the spermatozoa of normal boars. The ISTS defect is a manifestation of an autosomal recessive disease caused by an insertion in the KPL2 gene in porcine chromosome 16. Although we tried to map the KA defect, its aetiology remains unclear.     

饲粮维生素D添加形式对公猪繁殖性能的影响

本试验旨在研究饲粮维生素D添加形式对种用公猪繁殖性能的影响。选取16头18月龄的约克公猪,随机分成2组,2组饲粮中分别含有50μg/kg维生素D3(VD3)和25-羟基维生素D3(25-OHD_3),每组8个重复,每个重复1头猪。试验期16周。结果表明,与VD3组相比:1~16周,25-OHD_3组公猪精子活力和每次射精的有效精子数显著提高(P0.05),而精子畸形率显著降低(P0.05);第112天,25-OHD_3组血浆钙离子(Ca2+)和雌二醇含量及芳香化酶活性显著提高(P0.05),精清25-OHD_3、Ca2+、果糖含量和酸性磷酸酶的活性显著提高(P0.05);25-OHD_3组芳香化酶、维生素D 25-羟化酶、维生素D 24-羟化酶和维生素D受体基因的表达量显著升高(P0.05)。综上所述,与同等水平VD3相比,种公猪饲粮中添加25-OHD_3能更有效增加血浆维生素D含量,从而改善精子的形态和运动能力,提高公猪的繁殖性能。     

Use of injectable vitamin E and selenium-vitamin E emulsion in ewes and suckling lambs to prevent nutritional muscular dystrophy

Forty-eight Blackbelly X Dorset, 27 Finnish, 26 Finnish X Dorset, 28 Rambouillet and 8 Dorset Suffolk-sired lambs were used in this experiment. Three weeks before lambing, one-half of the ewes received a selenium emulsion (Se-E) containing .05 mg selenium and 3.7 IU of vitamin E/kg body weight (BW). A 2 X 3 X 2 factorial arrangement was used; lambs from either treated or nontreated ewes were randomly assigned irrespective of breed to one of six treatment combinations consisting of 0 or .025 mg/kg BW selenium (Se) injected at birth or two .025 mg/kg BW Se injections, one at birth and one 2 to 3 wk later, and two levels of injectable Vitamin E (E; 0 and 100 IU) given at birth. Both lambs and ewes were provided access to 75% concentrate diets supplemented with Se and E at recommended NRC levels. Plasma activity of creatine phosphokinase (CPK) was highest at 1 d of age and exhibited decreases (P less than .001) over time. In lambs, the E injection tended to decrease plasma activity of CPK. Plasma glutathione peroxidase activity was lowest at 1 d of age and increased over the course of the experiment but was unaffected by treatments (P less than .05). Plasma tocopherol concentration decreased (P less than .01) with time, with E therapy tending to increase tocopherol concentration. Differences in mean plasma tocopherol concentrations among breeds were also observed (P less than .01). Selenium concentration increased over time and with the E injection (P less than .01). An interaction between ewe and lamb Se-E treatments also was observed (P less than .10), with nontreated lambs from nontreated ewes exhibiting lower Se concentrations than treated lambs from injected ewes. An increase in lamb plasma Se concentration was noted in response to Se-E treatments (P less than .001). In the ewes, plasma tocopherol concentration was lower while Se concentration was higher at 18 d than at 1 d postpartum (P less than .01 and P less than .001, respectively). Milk Se concentration was lower at 18 d than at 1 d (P less than .001) and was higher (P less than .10) in Se-E-treated ewes.     

Growth, carcass traits, boar odor and testicular and endocrine functions of male pigs fed a progestogen, altrenogest

Crossbred (Chester White X Yorkshire X Duroc) boars were used to evaluate the effects of feeding a progestogen (altrenogest) on body growth, endocrine function (determined during feeding and after withdrawal of altrenogest), carcass composition, boar odor and testicular function (determined after a 30-d withdrawal from altrenogest). Boars from 18 litters were assigned at 12 wk of age to three treatments: 1) 18 control boars; 2) 18 boars fed altrenogest (20 mg/day) for 6 wk from 15 to 21 wk of age, followed by 30 d with no treatment; and 3) 18 boars castrated at 2 wk of age (barrows). Daily gains were greater (P less than .05) in boars fed altrenogest than in barrows through 21 wk of age but were lower (P less than .05) than those of control boars and barrows during the 30-d withdrawal period. Boars fed altrenogest weighed less (P less than .05) than control boars and barrows at 25 wk of age (at slaughter). Both groups of boars were similar in percentage of muscle and had less (P less than .05) backfat than barrows, whereas control boars had the largest (P less than .05) loineye areas. Based on evaluations by a trained sensory panel, intensity of boar odor in fat samples was similar for both groups of boars and was greater (P less than .05) than that for barrows. Weights of accessory reproductive glands and weight and sperm content of testes and epididymides were reduced (P less than .05) in boars fed altrenogest.(ABSTRACT TRUNCATED AT 250 WORDS)     

Assessment of the influence of dietary vitamin E on sows and offspring in three parities: reproductive performance, tissue tocopherol, and effects on progeny

Sixty crossbred (Yorkshire-Hampshire X Duroc) gilts were fed one of four corn-soybean meal diets fortified with .3 ppm Se and 0, 16, 33, or 66 IU of DL-alpha-tocopheryl acetate/kg. The study was conducted over a three-parity period to evaluate sow reproductive performance and the vitamin E tissue status of both sows and progeny at various time periods postcoitum and(or) postpartum. The basal diet averaged 8.4 mg of alpha-tocopherol/kg and .38 ppm of Se. Although litter size at birth was lowest (P less than .15) when sows were fed the basal diet, a higher incidence of agalactia when sows were fed the lower dietary vitamin E levels resulted in an increased (P less than .05) litter size at 7 d postpartum as dietary vitamin E increased. Sow serum alpha-tocopherol increased (P less than .01) at each measurement period as dietary vitamin E level increased. Colostrum and milk alpha-tocopherol concentrations increased (P less than .01) as dietary vitamin E level increased, and colostrum values were three to five times higher than at later milks. Colostrum alpha-tocopherol declined by parity from sows fed less than or equal to 16 IU/kg but was similar at each parity for sows fed greater than or equal to 33 IU/kg, resulting in a dietary vitamin E x parity interaction (P less than .01). The Se content of sow milk declined with parity but was not affected by dietary vitamin E level. Sow liver tocopherol at weaning (28 d postpartum) increased (P less than .01) as dietary vitamin E increased and increased with parity (P less than .05). Pig serum and liver alpha-tocopherol concentrations were elevated at birth and 7 and 28 d of age as sow dietary level of vitamin E increased. Upon weaning, pigs were fed a torula yeast-dextrose diet that contained 3.0 mg of alpha-tocopherol/kg and .32 ppm Se for a 28-d postweaning period. Liver and serum alpha-tocopherol concentrations declined during the postweaning period. Evidence of the vitamin E deficiency occurred at 28 d postweaning in the progeny from sows fed the basal diet or 16 IU of vitamin E; the incidence was more prevalent in the pigs from Parities II and III. These results suggest that a supplemental level of 16 IU of vitamin E/kg of diet was inadequate for the reproducing sow; higher levels are justified, particularly when females are retained in the herd for several parities.     

Linseed oil in boar's diet improved in vivo fertility and antioxidant status

The reactive oxygen species (ROS) which are produced during storage of boar semen are causing oxidative stress and leads to poor fertility. Also, tropical and sub-tropical weather condition adversely impacts the physicomorphological quality and fertility of boar sperm. The aim of this study was to examine the effects of feeding linseed oil to boar on its seminal attributes, sperm kinetics, biomarkers of antioxidant, fatty acid profile of seminal plasma (SP) and sperm and in vivo fertility. Six Hampshire crossbreed boars were fed with 90 ml linseed oil (LIN) whereas six Hampshire crossbreed boars were fed 90 ml canola oil (CON) for 16 weeks. Sperm quality was evaluated (60 ejaculates for each group; a total of 120 ejaculates) for motility, livability, abnormal morphology, acrosomal membrane integrity, hypo-osmotic swelling test (HOST) and sperm kinetic parameters by computer assisted semen analysis (CASA) at 0 h and at 72 h of storage at 17°C. Biomarkers of antioxidant (glutathione peroxidase; GPx, catalase; CAT, total antioxidant capacity; TAC) and malondialdehyde (MDA) were measured in SP and serum. Gas chromatography–mass spectrometry (GC–MS) was used for the estimation of fatty acid composition of SP and sperm. Boars fed with linseed oil had higher semen volume (p < .01) and more total sperm numbers (p < .01). Feeding linseed oil to boar enhanced seminal attributes (p < .05) at 0 h as well as at 72 h of storage. Linseed oil feeding (p < .01) improved biomarkers of antioxidants and significantly (p < .01) lowered the lipid peroxidation in serum and SP. Linseed oil feeding (p < .05) increased the proportion of alpha linolenic (ALA), arachidonic and docosahexaenoic (DHA) fatty acids in SP. The ratio of n-6 to n-3 fatty acids in sperm increased significantly (p < .01) in treatment group. Farrowing rate was significantly (p < .05) higher in treatment group. In conclusion, feeding linseed oil to boar improved the in vivo fertility, enhanced antioxidant capacity and increased the DHA content of SP and sperm.     

Effects of marginal selenium deficiency and winter protein supplementation on growth, reproduction and selenium status of beef cattle

Seventy-two Hereford X Simmental cows, averaging 498 kg in body weight and 5.2 yr of age, were used in a 2-yr study to ascertain if selenium (Se)-vitamin E (E) injections and winter protein supplementation would affect growth, reproduction and health of beef cattle maintained year-round on feedstuffs marginally deficient in Se (.03 to .05 mg/kg). Cows received either no injection or a mixture of 30 mg Se (as sodium selenite) and 408 IU E injected subcutaneously beginning 3 to 4 mo prepartum and at 60-d intervals throughout the 2-yr period. Calves born to Se-E treated cows were injected with 5.5 mg Se and 75 IU E/100 kg body weight at 60-d intervals beginning at 1 mo of age. Calves were born between December 30 and February 20 and cows were bred between March 20 and May 20. Cattle grazed pasture (.05 mg Se/kg) that consisted of orchardgrass, bluegrass and white clover during the fall, spring and summer. During winter (December 15 to May 2), cattle were fed corn silage (.03 mg Se/kg) supplemented with either: no protein supplement (control), soybean meal or a urea-corn mixture. Cows and calves receiving Se-E had higher (P less than .01) whole blood glutathione peroxidase (GSH-Px) activity and plasma Se concentrations than controls. Selenium-E injections reduced (P less than .05) calf death losses from 15.3% to 4.2% and slightly increased (P less than .10) adjusted calf weaning weights. Hemoglobin concentrations were higher (P less than .05) in Se-E-injected supplemented calves at 1 mo of age but not at 5 or 7 mo of age.(ABSTRACT TRUNCATED AT 250 WORDS)     

Effect of selenium on performance, serum selenium concentration and glutathione peroxidase activity in pigs

Pigs from sows fed a diet deficient in Se and low in vitamin E were fed a Torula yeast diet supplemented with 100 IU dl-alpha-tocopheryl acetate/kg of diet. Dietary treatments were levels of supplemental Se of 0, .025, .050, .075 or .100 ppm. Some death loss occurred in pigs receiving no supplemental Se at approximately 5 wk of age. Autopsy revealed liver and heart lesions typical of vitamin E-Se deficiency. Selenium supplement had no significant effect on average daily gain, feed intake or gain to feed ratio for the 4-wk experiment. Selenium status of pigs was determined by serum Se concentration and serum glutathione peroxidase (GSH-Px) activity. Serum Se increased linearly (P less than .01) with increasing supplemental Se. Serum GSH-Px activity increased linearly (P less than .01) and quadratically (P less than .05) with increasing supplemental Se. With time, the level of serum Se and GSH-Px activity decreased in unsupplemental pigs, but increased in pigs fed diets supplemented with Se and resulted in significant interactions (P less than .01) between dietary Se level and time on experiment. The correlation between serum Se concentration and GSH-Px activity was .81 (P less than .01).     

Testicular development and endocrine characteristics of boars selected for either high or low testis size

Thirty-six Landrace x Large White cross boars were selected from litters with either high or low estimated breeding values for 150-d paired testis weight. Blood samples were taken via jugular venipuncture at eight ages (42, 56, 70, 84, 98, 112, 126 and 140 d). At each sampling age, nine blood samples were taken at 30-min intervals. Luteinizing hormone (LH) was determined on the individual serum samples. Serum samples from each boar at each age were pooled and concentrations of follicle-stimulating hormone (FSH), estradiol-17 beta (E2) and testosterone (T) were determined. Paired testis width, testis length and body weight were measured at 98, 112, 126 and 140 d of age. Backfat probe, weights of excised testes and histological data on testes were obtained at 140 d of age. Boars with high testis weight (HTW) were heavier (P less than .05), had higher adjusted backfat probes (P less than .01) and had consistently larger in situ testis measurements (P less than .01) than did low testis weight (LTW) boars. Boars with HTW had heavier (P less than .01) testes and epididymides at 140 d of age. They also had a higher percentage of seminiferous tubules in which spermatogenesis was present (P less than .05), a larger percentage of tubules with a lumen (P less than .05) and tubules had a larger mean diameter (P less than .01) than did those of boar with LTW. Adjustment of in situ testis measurements and excised testis weights for body weight reduced line differences by less than 20%. A rise in LH concentrations occurred at approximately 100 d of age. Boars with HTW had higher (P less than .05) and more variable (P less than .01) LH concentrations than did boars with LTW. Boars with HTW also had higher maximum concentrations of LH during the pubertal rise (P less than .01) and these concentrations tended to reach maximum levels at younger ages. Concentrations of T increased in a fashion that was nearly linear with age (P less than .01) and tended to be higher for the boars with HTW (P less than .10). Concentrations of E2 changed little from 42 to 84 d of age but increased steadily thereafter. Boars with HTW had a more rapid increase in E2 concentrations than did boars with LTW (P less than .05).(ABSTRACT TRUNCATED AT 400 WORDS)     

Numbers of Sertoli cells, quantitative rates of sperm production, and the efficiency of spermatogenesis in relation to the daily sperm output and seminal quality of young beef bulls

Data from 34 yearling Hereford or Angus bulls were used to investigate relationships of testicular size, quantitative rates of sperm production, Sertoli cell numbers, numbers of germ cells supported per Sertoli cell, and the efficiency of spermatogenesis to daily sperm output and seminal quality. Two ejaculates were collected by electroejaculation from each bull on each of 2 days/week throughout the study. The percentage of progressively motile sperm and the percentage of morphologically normal sperm were determined from aliquots of fresh semen. Additional aliquots of semen were frozen in glass ampules or plastic straws and subsequently evaluated for postthaw motility and percentage of sperm with intact acrosomes. Sertoli cell numbers, the numbers of germ-cells per Sertoli cell, and the efficiency of spermatogenesis were unrelated to the quality of fresh or frozen semen (P greater than 0.05). In first ejaculates, the numbers of sperm and motile sperm were related (P less than 0.05) to testicular parenchymal weight (r = 0.38 and 0.50), daily sperm production (r = 0.45 and 0.53), and spermatids per gram of testicular parenchyma (r = 0.35 and 0.34). Testicular parenchymal weight and daily sperm production also were related to daily sperm output and to the average daily motile sperm output of these bulls (P less than 0.05), but could account for less than 25% of the variability in these end points among bulls.     

Effect of divergent selection for testosterone production on testicular morphology and daily sperm production in boars

The objective of this study was to characterize correlated responses in testicular morphology and daily sperm production to divergent selection for testosterone production. Duroc boars from high and low lines (HTL and LTL, respectively) divergently selected over 10 generations for testosterone production in response to a GnRH challenge followed by random selection were used. Testicular tissues were sampled from all available males of generation 20 (HTL, n = 46; and LTL, n = 13). Volume densities for Leydig cells, seminiferous tubules, and Sertoli cells were estimated along with sperm production. The HTL boars had greater volume densities of Leydig cells than did LTL (P < 0.01). Volume density of seminiferous tubules tended to differ between lines (P < 0.07), but Sertoli cell volume densities did not differ (P < 0.27). Sperm production traits, adjusted for age, did not differ significantly between lines. Body, testicular, and epididymal weights were recorded for boars from HTL (n = 82) and LTL (n = 44) from generations 20 and 21. After adjustment for BW, average paired testicular weights for HTL and LTL were 417 and 457 g (P < 0.01), respectively. Epididymal weights, adjusted for BW, were heavier for HTL (P < 0.01) than for LTL. To demonstrate that the selection lines still differed for testosterone production, lines were evaluated in generation 21. Endogenous testosterone production of the HTL (n = 54) and LTL (n = 44) testosterone production line averaged 49.0 ng/mL and 27.8 ng/mL (P < 0.01), respectively. Plasma FSH concentrations did not differ between lines (P < 0.30). Selection for testosterone production in response to a GnRH challenge was an effective method of changing testosterone concentrations, testicular size, epididymal weight, and volume density of Leydig cells. However, daily sperm production per gram of testes was unchanged. Based on the results of this study, selection for testosterone production is not recommended as a method of increasing sperm production in pigs.