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1.
European sea bass exhibits a feeding rhythm with seasonal phase inversions: nocturnal feeding is predominant in winter, and diurnal feeding during the rest of the year. This explains the increased growth of sea bass fed at night during the winter months, but to fully assess such feeding practices, it is important to monitor food-catching efficiency in the dark. To evaluate the influence of nocturnal feeding on pellet-capture efficiency under laboratory conditions, we developed an infrared pellet detector linked to on-line recording with a microcomputer. Feed waste was recorded for eight groups of four sea bass (54.6±15.8 g body weight) fed either diurnal or nocturnal meals with long (>60 s) or short (<20 s) feed availability times. With short feed availability times, food-catching efficiency in complete darkness (0 lx) was reduced to 78.6%, compared to 93.5% in diurnally fed fish. This was confirmed in a second experiment, performed during winter, involving 4000 sea bass in four sea cages (1000 per cage) provided with self-feeders and pellets detectors. Feed waste amounted to less than 13% of the food distributed, but nocturnal demands (29% of total feed demands) were accompanied by higher levels of uneaten pellets (68% of the total feed waste) than diurnal demand. This suggests that if the aim is to reduce feed waste, nocturnal feeding should be restricted in sea cages. 相似文献
2.
Osteological malformations are always considered an important problem in intensive aquaculture. This work studies the effect of rearing temperature on malformations in sea bass, Dicentrarchus labrax. To this end, two batches of the species were subjected to the following incubation/cultivation temperatures: 15 °C/natural and 19/19 °C, from fertilization until 190 days after hatching. The different malformations were studied in 1643 juveniles at 190 days and classified by categories. The Chi-square statistic was calculated to verify the relationship between the presence of anomalies and the application of different temperatures. The percentage of anomalies observed in individuals reared at a high temperature (19/19 °C) was 66.44%. In both temperature systems, opercular malformations were those which caused a greater delay in growth. The results found indicated that temperature played a very important role in the development of deformations, which may be of interest from the viewpoint of aquaculture. 相似文献
3.
The combined effect of feeding time and meal size on the growth performance and feeding rhythms were studied in European sea bass maintained under natural summer–autumn conditions. Three feeding strategies were compared: a modulated automatic-feeding (MF), a fixed automatic-feeding (FF) and a free access to self-feeders (SF). Under MF, feed was supplied in meals of different size, three times a day (morning, afternoon and evening, 33.33:16.67:50% of daily feed ration, respectively) during the first (31 days) and second (35 days) period of the experiment, and twice a day (morning and evening, 33.33:66.67% of daily feed ration, respectively) during the third period (27 days). In FF feed was supplied in equally-sized meals, three times a day (morning, afternoon and evening, 33.33:33.33:33.33% of daily feed ration) in all three periods of the experiment. Under SF, fish showed a diurnal self-feeding pattern, with the greatest percentage of self-feeding activity concentrated in the evening. In MF and FF, although feed was delivered automatically, the trigger was left in the tank to register activations as an indicator of feeding activity. The trigger activation of both treatments MF and FF was associated with the time of feed delivery. Feeding strategies affected biomass increase, specific growth rate (SGR) and feed conversion ratio (FCR), the greatest biomass increase and highest SGR being obtained with MF and the poorest FCR with FF. The results demonstrate that automatic-feeding systems, in which the quantity of feed supplied is modulated in accordance with the natural feeding rhythms of sea bass, may improve growth and feed efficiency. 相似文献
4.
A pharmacokinetic study of oxytetracycline (OTC) following an intravascular administration (40 mg/kg) was carried out in sea bass, Dicentrarchus labrax (110 g), at 13.5 and 22 °C water temperature. Blood, muscle and liver samples were taken at 1, 2, 4, 8, 16, 32, 64 and 128 h post-injection. The plasma data were conformed to a two-compartment model. The kinetic profile of the drug was found to be temperature dependent. The absorption half-life ( t1/2) of OTC was 0.98 and 0.192 h at 13.5 and 22 °C, respectively, whereas the elimination half-time ( t1/2β) of the drug was 69 h at 13.5 °C and 9.65 h at 22 °C. The apparent volume of distribution of the drug at steady state [ Vd(ss)] was 5.62 l/kg at 13.5 °C and 2.59 l/kg at 22 °C. The mean residence time (MRT) of OTC was found to be 37.7 h at 22 °C and 71 h at 13.5 °C. The total clearance of the drug (CL T) was calculated to be 73.5 and 68.7 ml/kg/h at 13.5 and 22 °C, respectively. Liver levels indicated higher OTC values than respective muscle levels at all time points and for both temperatures. The elimination of OTC from tissues tested was faster at the high temperature, whereas the drug was eliminated faster from liver compared to muscle when comparisons are made at the same temperature. 相似文献
5.
Meiotic gynogenetic and triploid sea bass were produced by pressure shocks according to a previously published protocol. Pressure-treated groups did not survive as well as controls during early development and larval rearing. Performances, sexual maturation and carcass quality were examined over a period of 34–45 months. At the age of 34 months, growth of the gynogenetic fish was comparable to that of the control but inferior in the triploid fish. A predominance of male fish was found within the triploid groups, while diploid and meiotic gynogenetic fish showed equal proportions of the sexes. Gonadal maturation in triploid fish was significantly impaired, particularly in the females that showed rudimentary ovaries. Triploid males exhibited primary maturation but proved to be gametically sterile. Pressure-induced triploids did not grow as well as diploids, but these results might be ascribed to specific on-growing conditions (communal rearing). The performance of gynogenetic sea bass was comparable to that of control. The superiority of diploid fish over their triploid counterparts was confirmed during the final growing period and more clearly so in females. Performances of triploids varied according to their maternal origin. Overall, striking qualitative differences between diploid and triploid fish were found at the age of 34 and 45 months, although the results varied in a gender-specific manner. A strong maternal effect was also observed. The potential advantages of triploid sea bass for aquaculture purposes are discussed. 相似文献
7.
The aim of this trial was to study the utilization of dietary protein by seabass juveniles with 5.5 g mean body weight, at two water temperatures: 18°C and 25°C. For that purpose, the fish were fed for 12 weeks, four isoenergetic diets with different protein levels (36, 42, 48, and 56%). At the end of the trial, growth rate and feed utilization were significantly better at the higher water temperature. Within each temperature, specific growth rate and feed efficiency were significantly higher with the 48 and 56% protein diets than with the other diets. At 25°C, feed efficiency was also significantly better with the 56% than with the 48% protein diet. N retention (g kg average body weight −1 day −1) was higher at 25°C than at 18°C but, as a % N intake the inverse was true. Although at 25°C N retention (% N intake) was not different among groups, retention in g kg ABW −1 day −1 was significantly higher with the 56% protein diet than with 36 and 42% protein diets. On the contrary, at 18°C N retention (g kg ABW −1 day −1) was similar among groups while as a percentage of N intake it was inversely related to the dietary protein level. Regarding energy utilization, at each temperature, there were no differences among dietary treatments on energy retention (g kg ABW −1 day −1). As a % of energy intake, energy retention significantly increased with the increase of dietary protein level at 25°C, while at 18°C, there were no significant differences among groups. Within each temperature, at the end of the trial, there were no differences among groups in proximate composition of whole fish. Apparent digestibility coefficients of dry matter, protein and energy significantly improved with the increase of water temperature but, within each temperature, there were no significant differences among groups. The results of this study indicate that, regardless of water temperature, the dietary protein requirement for growth seems to be satisfied with a diet containing 48% protein. Growth and feed efficiency were significantly higher at the higher temperature, however, protein utilization was more efficient at the lower temperature. 相似文献
8.
European sea bass juveniles (14.4±0.1 g mean weight) were fed diets containing different levels of fish oil then of n-3 highly unsaturated fatty acids (n-3 HUFA) for 12 weeks. The fish performance as well as fatty acid (FA) composition of neutral and polar lipids from whole body after 7 and 12 weeks feeding were studied. The requirements of juvenile sea bass for n-3 highly unsaturated fatty acids (n-3 HUFA) were studied by feeding fish diets containing six different levels of n-3 HUFA ranging from 0.2% to 1.9% of the diet, with approximately the same DHA/EPA ratio (1.5:1). The growth rate at the end of the trial showed significant differences. Fish fed low dietary n-3 HUFA (0.2% DM of the diet) showed significantly lower growth than the diet 3 (0.7%), then no further improvement (P>0.05) of growth performance was seen by elevating the n-3 HUFA level in the diet up to 1.9% (diet 6). No difference in feed efficiency, protein efficiency ratio or protein retention was observed among treatments, nor in protein and total lipid content. However, the n-3 HUFA levels in diets highly influenced fish fatty acid composition in neutral lipid, while polar lipid composition was less affected. Comparison of polar lipid content after 7 or 12 weeks indicated that DHA remained stable at the requirement level, while arachidonic acid decreased with time. Results of this experiment suggest that the requirement for growth of n-3 HUFA of juvenile sea bass of 14 g weight is at least 0.7% of the dry diet. 相似文献
9.
To examine the effects of temperature on sex differentiation in the black sea bass ( Centropristis striata L.), a protogynous hermaphrodite, juveniles (∼0.5 g) were cultured in recirculating systems at 17, 21 or 25 °C. Growth was assessed at 155, 182, 241 and 275 days post hatch and sex differentiation was determined histologically. No differences were found in the sex ratios of fish reared at different temperatures, but only 55–64% developed as females. Growth was significantly greater in males across all temperature treatments. These results suggest that black sea bass exhibit sexually dimorphic growth patterns and that female-specific sex determination can be disrupted in culture. 相似文献
10.
在海水循环水养殖系统中,分别设置白光(λ_(400~780 nm))、红光(λ_(630 nm))、黄光(λ_(595 nm))、绿光(λ_(530 nm))、蓝光(λ_(455 nm))5组不同LED光谱处理组,研究5种不同LED光谱对舌齿鲈幼鱼[(29.91±0.39) g、(13.78±0.35) cm)]摄食、生长和能量分配的影响。结果显示,不同LED光谱对舌齿鲈幼鱼摄食、生长和能量分配具有显著性影响。红光谱下舌齿鲈幼鱼生长较好(41.09±5.70) g,蓝光谱下舌齿鲈幼鱼生长较差(36.02±4.18) g;蓝光谱下舌齿鲈幼鱼DGC(daily growth coefficient)显著低于其他各组,其他各组之间差异不显著;5组LED光谱处理组舌齿鲈幼鱼摄食率存在显著性差异,其中红光谱摄食率最高。类胰岛素生长因子IGF-1和IGF-2测定结果表明,白光谱下IGF-1的mRNA表达量最高;黄光谱下IGF-2的mRNA表达量最高。能量分配方面,红光谱下舌齿鲈幼鱼排粪能和代谢能较低,摄食能和生长能较高;蓝光谱下舌齿鲈幼鱼排粪能较高,摄食能和代谢能较低,生长能最低。对舌齿鲈幼鱼肌肉和肝脏RNA/DNA比值测定结果表明,蓝光谱下,舌齿鲈幼鱼肝脏RNA/DNA比值显著高于其他各组;蓝光谱和白光谱下舌齿鲈幼鱼肌肉中RNA/DNA比值存在显著性差异,其他各组之间不具有显著性差异。研究表明,红光谱下舌齿鲈幼鱼摄食、生长均表现较好,且红光谱下舌齿鲈幼鱼具有较高的摄食能和生长能;蓝光谱下舌齿鲈幼鱼摄食、生长表现较差,排粪能较高,生长能较低。因此,红光谱对舌齿鲈幼鱼摄食、生长具有积极影响;蓝光谱对舌齿鲈幼鱼摄食、生长具有不利影响。 相似文献
11.
Juvenile European sea bass from the same fish stock were selected by successive size grading processes using 2, 3 and 4 mm bar graders at 79, 96 and 99 days post hatching, thus forming three groups ( n=300) consisting of similar‐sized fish that differed by time of each group formation. The growth patterns of fish groups were studied at three temperatures during 5 weeks of rearing. Three‐way anova followed by the Tukey multiple comparison test ( P<0.05) showed a high dependence of growth on the temperature applied. The smallest size and weight of fish were detected in all groups reared at 19 °C compared with fish held at 21 and 23 °C respectively. Differences in coefficients of variation of lengths were small and insignificant between groups and temperatures. Growth in the length of sea bass juveniles during the test period was a linear function of time and no differences were observed in growth rate among groups at a particular temperature. Growth rates of fish were 0.71 ± 0.02, 0.62 ± 0.01 and 0.52 ± 0.02 mm day ?1 at 23, 21 and 19 °C respectively. These results indicated that the variations in body size of juveniles in the test period were not the result of differences in the growth potential of individuals. 相似文献
12.
密度胁迫会对水生动物的免疫和抗氧化功能等产生负面影响。为提高拟穴青蟹 ( Scylla paramamosain) 养殖产量潜力,为其养殖业发展提供理论依据,通过将拟穴青蟹置于8 只·m −2 (低密度组)、16 只·m −2 (中密度组)、32 只·m −2 (高密度组) 3种不同密度条件下养殖72 h,研究了密度对拟穴青蟹抗氧化和免疫能力的影响。抗氧化酶活结果表明:高密度组过氧化氢酶 (CAT) 和超氧化物歧化酶 (SOD) 活性均显著高于低密度和中密度组 ( P<0.05),而谷胱甘肽过氧化物酶 (GSH-Px) 活性在各组之间无显著性差异;高密度组丙二醛 (MDA) 含量与低密度和中密度组相比显著升高。免疫酶活结果表明,高密度组酸性磷酸酶 (ACP)、碱性磷酸酶 (AKP) 和溶菌酶 (LZM) 活性均显著低于低密度和中密度组 ( P<0.05),而中密度组ACP、AKP、LZM活性与低密度组无显著性差异。荧光定量结果显示,高密度组热休克蛋白70基因 ( HSP70) 表达水平呈先降低后升高趋势,且在养殖第6—第48小时均显著低于低密度和中密度组 ( P<0.05);高密度组 Caspase 3基因表达水平在养殖6 h后显著升高,在第24小时达到最高后逐渐下降,但其表达水平始终显著高于低密度组 ( P<0.05)。综上,高密度养殖会对拟穴青蟹造成氧化损伤,进而导致其免疫能力下降。16 只·m −2的养殖密度对拟穴青蟹抗氧化和免疫能力无显著影响。 相似文献
13.
本文就温度,盐度以及不同降低盐度方法对鲈于仔鱼的生长及存活进行实验,经一星期的培养,结果表明,1;钙鱼仔鱼在盐度30-3‰的海水中都能生长,以20‰的存活率最高达99%。2.每经6小时逐渐降低盐度5‰的仔鱼存活率高于大幅度骤降盐度。3.温度14.7-14.9℃和18.3-19.7℃,以盐度30‰生长快,5‰生长缓慢。 相似文献
14.
Whole-animal thyroxine (T 4) and 3,5,3′-triiodothyronine (T 3) levels were measured in larval and juvenile striped bass, Morone saxatilis, reared for 10 days at one of three levels of salinity (equivalent to fresh water (FW), one-third seawater (1/3 SW), and
seawater (SW) and two temperatures (15°C and 20°C). The striped bass were pre-metamorphic larvae, metamorphic larvae or juveniles.
The short-term effects of seawater on plasma T 4 levels of juvenile striped bass were also measured. Higher salinities increased T 4 levels in premetamorphic larvae. In metamorphic larvae, SW and 1/3 SW increased T 4 levels and SW increased T 3 levels at 20°C. This response was eliminated in those at 15°C. Whole-animal thyroid hormone content was unaffected by salinity
or temperature in juvenile striped bass, although significant fluctuations in plasma T 4 levels occurred in those transferred to 1/3 SW and SW. The thyroid axis of striped bass responds to salinity and temperature
as early as in the pre-metamorphic stage. Thyroid hormones may mediate the beneficial effects of salinity on larval striped
bass growth and survival. 相似文献
15.
In this paper we investigate the interleukin-10 (IL-10) expression and 3D modelling of the European sea bass ( Dicentrarchus labrax L.). IL-10 is a regulatory cytokine that has been intensively studied in mammals and has been found mainly involved in the suppression or deactivation of activated immune responses. The full-length sea bass IL-10 cDNA consists of 1015 bp and is translated in a predicted molecule of 187 amino acids. A multiple alignment of this peptide with other known sequences showed the conservation of fundamental IL-10 family characteristics. Expression analysis by real-time PCR in control fish showed a high basal expression in the head kidney (HK), followed by gut and brain. In vitro treatment of HK leucocytes with LPS showed an intense increase of IL-10 expression at 4 h and a significant decrease at 24 h, with PHA-L no differences were evidenced. A 3D model for sea bass IL-10 was obtained by accurate homology procedures and putative interaction residues with the IL-10 receptor described. The results suggested that sea bass IL-10 could be involved in anti-inflammatory responses, but further experiments are needed to assess its importance in response to pathogenic agents, vaccinations and immunostimulants. 相似文献
16.
For large-scale seed production of sea cucumbers through a hatchery system, it is imperative to know the effects of environmental parameters on larval rearing. Auricularia larvae (48 h post-fertilization) were obtained from induced spawning of Holothuria spinifera and used in experiments to ascertain the effects of temperature, salinity and pH on the growth and survivorship of the larvae. The larvae were reared for 12 days at temperatures of 20, 25, 28 and 32 °C; salinities of 15, 20, 25, 30, 35 and 40 ppt; and pH of 6.5, 7.0, 7.5, 7.8, 8.0, 8.5 and 9.0. The highest survivorship and growth rate and fastest development of auricularia indicated that water temperature of 28–32 °C, salinity of 35 ppt and pH of 7.8 were the most suitable conditions for rearing larvae of H. spinifera. 相似文献
17.
Black sea bass Centropristis striata (L.) juveniles were reared in aquaria containing either shelter or no shelter to investigate the effects of shelter on growth and survival. The specific growth rates of juveniles were significantly higher in the sheltered aquaria. With shelter present, the average mortality of juveniles caused by agonistic behaviour was 44% compared with no mortality in the unsheltered aquaria. The results provide evidence that shelter is advantageous for growth, but not survival of black sea bass juveniles under culture conditions. 相似文献
18.
A 21-day feeding trial was carried out to investigate the ability of first feeding European sea bass larvae to utilize medium-chain triacylglycerols as an alternative source of energy. Three compound diets based on soluble fish protein concentrate and yeast were supplemented with either 3% tricaproin (TC6), tricaprylin (TC8) or tricaprin (TC10). A diet containing triolein (TOL) was used as a reference diet. Diets were tested on four replicate groups of first feeding European sea bass larvae at 20°C, i.e. 6 days after hatching. At the end of the 21-day trial, TC8 yielded significantly higher survival (57±8% vs. 28±11% for the three other groups). Considered together, larvae fed TC8 and TC6 displayed better growth rates than larvae fed TOL and TC10 (final mean wet weights: 1.5±0.3 mg vs. 1.2±0.2 mg, respectively). The fatty acid composition of larval total lipid revealed a low deposit of medium-chain fatty acids (between 1 and 3% of total fatty acids) suggesting that medium-chain fatty acids were oxidized for energetic purposes. Tricaprylin and to a lesser extent tricaproin, appear to be potential energy sources for first feeding European sea bass larvae reared on compound diets. 相似文献
20.
The compensatory growth response of the European sea bass ( Dicentrarchus labrax) that faced cycling starvation and restricted ration was assessed. Juveniles (10.5 g) were stocked into 15 tanks at a density of 25 fish per tank. Five different feeding regimes were tested on triplicate groups of fish: C Satiation: control fed for 60 days without deprivation, C Restricted: 25% restricted feeding, S‐R: 1 day starvation then 4 days C Restricted feeding, R‐F: 1 day C Restricted feeding then 4 days C Satiation feeding, and, finally, S‐F: 1 day starvation then 4 days C Satiation feeding. The specific growth rate of fish in the C Satiation (2.5 ± 0.06% day ?1), S‐F (2.5 ± 0.11% day ?1) and R‐F (2.4 ± 0.18% day ?1) were significantly higher than that of C Restricted (2.2 ± 0.05% day ?1) or S‐R (2.0 ± 0.01% day ?1). Fish in S‐F group were able to achieve catching up with the C Satiation. There was no significant difference in feed conversion rates but R‐F and S‐F consumed approximately 34% more feed than C Satiation following the first re‐feeding day. Although, the highest lipid content was observed in C Satiation (14.4%), S‐R (33.3% dry matter) had the highest water content compared with the C Satiation and C Restricted (37.8% and 36.9% dry matter respectively). In conclusion, it may be concluded that sea bass has rapid response to cycling starvation/re‐feeding and that a 25% restricted feeding ratio is insufficient to invoke a compensatory growth response in sea bass. 相似文献
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