襄樊地区甜菜夜蛾的发生规律与综合防治

甜菜夜蛾（Spodoptera exigua Hubner）又名贪夜蛾,属鳞翅目夜蛾科,是一种世界性、杂食性、暴发性、易产生抗药性的害虫,在我国长江流域为害较为严重。近年来,随着蔬菜面积的扩大及复种指数的增加,甜菜夜蛾为害逐年加剧,现已成为为害襄樊地区十字花科、豆科、茄科等蔬菜的主要害虫。     

花椰菜主要害虫的综合防治

危害花椰菜的害虫种类较多,主要有蚜虫、白粉虱、小菜蛾、菜粉蝶、甜菜夜蛾等,近几年尤以白粉虱、小菜蛾、甜菜夜蛾为害严重。现将花椰菜主要害虫及其防治技术总结如下: 1 为害特征     

大棚芦笋害虫性诱剂诱杀技术

斜纹夜蛾、甜菜夜蛾是浙北芦笋产区的主要灾害性害虫,每年7～10月为害最重。两虫的特点是发生突然性、暴食性和偏嗜性,假死、昼伏夜出,低龄幼虫群集和高龄幼虫分散为害等。特别是甜菜夜蛾对常规药剂产生抗性,药剂防治十分困难,为提高防治效果,现利用性诱剂对害虫进行诱杀防治,减少田间落卵量,     

九种药剂防治甜菜夜蛾等夜蛾类害虫试验

甜菜夜蛾是鳞翅目夜蛾科的一种杂食性害虫,为害多类作物,在南京地区常年发生4～5代,属夏秋多发型,但不同年度间歇发生,重发年份可对作物造成严重损失。由于以甜菜夜蛾为主的夜蛾类害虫食性杂、世代重叠现象严重、抗药性上升快等原因,防治难度很大。2002年9月13日选用9种药剂开展了防治甜菜夜蛾试验,并将防治时间适当提前到甜菜夜蛾卵孵始盛期,以探索不同药剂对甜菜夜蛾等夜蛾类害虫的防治效果。1材料与方法1.1供试药剂①10%除尽EC,德国巴斯夫公司产;②52.25%农地乐EC,美国陶氏益农公司产;③20%米满SC,美国罗门哈斯公司产;④24%美满SC,美…     

性诱剂防治斜纹夜蛾、甜菜夜蛾试验

斜纹夜蛾、甜菜夜蛾是浙江慈溪蔬菜产区的主要灾害性害虫,每年7～10月为害最重.两虫的特点是:寄主作物广,发生突然性,暴食性和偏嗜性,假死、昼伏夜出,低龄幼虫群集和高龄幼虫分散为害等特性.斜纹夜蛾寄主作物多达45科100余种,甜菜夜蛾为30余种.特别是甜菜夜蛾对常规药剂产生抗性,药剂防治十分困难,为探索新的防治途径,特开展了性诱剂防治试验,现报道如下.     

甜菜夜蛾发生规律及综合防治技术

甜菜夜蛾是杂食性害虫,可危害葱、白菜、甘蓝、辣椒、甘薯等多种农作物。近年来,随着农田生态环境的变化,特别是气候变暖,使甜菜夜蛾的发生愈来愈重。由于该虫具有暴发成灾的特性,常造成毁田绝产。2007年,甜菜夜蛾在陕西省华阴市发生早,大面积暴发成灾,为害时间长,虫口密度高,为害作物范围广,给生产造成了极大的损失。笔者对其为害特征、发生规律及防治技术进行了研究,现介绍如下：     

甜菜夜蛾发生规律及综合防治技术

甜菜夜蛾是杂食性害虫,可危害葱、白菜、甘蓝、辣椒、甘薯等多种农作物.近年来,随着农田生态环境的变化,特别是气候变暖,使甜菜夜蛾的发生愈来愈重.由于该虫具有暴发成灾的特性,常造成毁田绝产.2007年,甜菜夜蛾在陕西省华阴市发生早,大面积暴发成灾,为害时间长,虫口密度高,为害作物范围广,给生产造成了极大的损失.笔者对其为害特征、发生规律及防治技术进行了研究,现介绍如下:     

甘蓝夜蛾的危害及防治

一、分布与为害 甘蓝夜蛾(Barathra brassicaeL.)又名甘蓝夜盗蛾,各地都有分布,以北方发生较重。 甘蓝夜蛾是多食性害虫,主要为害甘蓝、白菜等十字花科蔬菜,以及瓜类、豆类、茄果类蔬菜和甜菜等,其中以甘蓝、秋白菜、甜菜受害最重。幼虫共6龄,具有群集性、夜出性、暴食性。初孵幼虫集中在叶背取食,啃食叶肉,使叶片残留表皮,     

南京地区蔬菜夜蛾类害虫发生特点及防治技术

斜纹夜蛾和甜菜夜蛾是南京地区蔬菜生产上主要夜蛾类害虫 ,自 2 0世纪 90年代以来 ,2种夜蛾的种群数量愈益加重 ,暴发的频率提高 ,现已演变为常发性害虫。为此 ,笔者通过几年来田间调查观察 ,对2种夜蛾类害虫的发生特点和防治技术作了探索 ,实践中取得了较好的效果。1　田间发生调查1.1　斜纹夜蛾　斜纹夜蛾在南京地区一般每年发生 2～ 3代 ,主害代为第 2代和第 3代 ,为害夏秋季蔬菜。据田间调查 ,1代于 7月 30～ 31日出现幼虫高峰 (1996年 ) ;2代一般出现两个幼虫高峰 ,即 8月 7日至 12日和 8月 2 0日至 2 6日 ,迟的年份为 9月 6～ 13日 ;3…     

甜菜夜蛾性信息素研究及应用进展

甜菜夜蛾是我国多发的一种蔬菜害虫,化学农药的大量使用造成其抗药性大幅上升,防治效果明显下降。因此,安全有效的生物防治已成为当前的研究热点,其中利用昆虫性信息素进行害虫的测报与防治受到了广泛的关注和重视。现综述甜菜夜蛾性信息素的研究现状、性信息素在测报与防治中的应用以及利用性信息素的优点和局限性。     

我国甜菜夜蛾迁飞与越冬规律研究进展与趋势

我国自20世纪90年代开始对甜菜夜蛾迁飞与越冬规律及行为机制进行系统研究,明确了甜菜夜蛾不同于粘虫等典型迁飞害虫的迁飞模式,揭示了其迁飞、越冬与生殖三者之间的有机联系。综述了甜菜夜蛾迁飞与越冬最新研究成果,对其进一步的研究方向及趋势进行了探讨。     

The activin receptor-like kinase Ⅰ promotes proliferation and migration in HUVECs

AIM: To study the function of activin receptor-like kinase Ⅰ (ALK1) gene in vascular endothelium. METHODS: The human umbilical vein endothelial cells (HUVECs) were cultured, and the change of expression of ALK1, ALK5 in activation of HUVECs was analyzed. The full-leng coding sequence of ALK1 was cloned into pcDNA3.1+using standard protocols. The constructed pcDNA3.1+ALK1 plasmid were transfected into HUVECs. The proliferation and migration of HUVECs were detected by boyden champer and flow cytometry. RESULTS: The expression of ALK1 was up-regulated in resolution. ALK1 promoted the proliferation and migration of HUVECs. CONCLUSION: ALK1 has an important function in remodeling by promoting the proliferation and migration of endothelial cells.     

Progress in the research of mechanism underlying the inhibitory effect of thrombospondin-1 on tumor angiogenesis

Inhibition of tumor neovascularization (angiogenesis) has become one of the novel thera peutic strategies for tumor, and natural inhibitor of angiogenesis has been highlighted in this field. Thrombospondin-1 (TSP-1), as an important extracellular matrix component which can influence endothelial cel adhesion, migration, and proliferation, is reported to be a potent inhibitor of angiogenesis in vivo. It wil be valuable to use its active peptides or small fragments from TSP-1 in antiangiogenesis therapy.     

Effect of fibrinogen,fibrin and fibrin degradation products on the proliferation and migration of human vascular smooth muscle cells

AIM: To investigate the effect of fibrinogen (Fg), fibrin (Fb) and fibrin degradation products (FDPs) on the proliferation and migration of human vascular smooth muscle cells (VSMC).METHODS: The effects of Fg, Fb and FDPs on the proliferation of VSMC were observed by means of cell counting and MTT test, migration assays were performed using the wounding model and the transwell cell culture apparatus.RESULTS: Fg itself did not stimulate the proliferation of VSMC, but stimulated VSMC migration. Fb and FDPs both stimulated the proliferation and migration of VSMC, meanwhile the effect of Fb was in a dose-dependent manner.CONCLUSION: Fb, in particular FDPs, may play an important role by stimulating the proliferation and migration of VSMC in restenosis and atherogenesis.     

Role of angiotensin II and angiotensin II receptors in vascular smooth muscle cells migration

AIM:To determine the effects of Angiotensin II(AngII) on migration of rat smooth muscle cells and to investigate the mechanisms underlying Ang II action in the development of injured vascular disease. METHODS:VSMCs isolated from aortic media of Wistar rats and cultured by the modified explant method were adopted. In prersence and absence of AngII, the expression of AngII receptor and reorganization of the actin cytoskeleton of VSMCs were studied by immunocytochemistry technique, fluorocytochemistry technique. The migration assays were performed by a modified Boyden's chamber. And the effects of AT₁R antagonist (CV-11974), AT₂R antagonist (PD123319) on aforementioned target were studied.RESULTS:VSMCs migration was stimulated by addition of AngII. The dynamic reorganization of actin cytoskeleton may be an important mechanism by which AngII facilitates VSMC motility. The expression of AT₁R in VSMCs can be upregulated after treatment with AngII initially, then decreased gradually. The expression of AT₁R was downregulated by AT₁R antagonist. The effect of AngII on VSMCs migration was mediated by AT₁R, while AT₂R had no significant effect.CONCLUSION:The dynamic reorganization of actin cytoskeleton is required for AngII-induced VSMC migration, and this effect is mediated by AT₁R .     

Fractalkine and CX3CR1 have intimate relationships with chemotaxis and adhesion of leukocyte

Fractalkine has been recognized as the first member of the fourth chemokine family recently and its receptor, CX3CR1, was ident ified afterwards. This pair of ligand/receptor play important roles in the migration and adhesion of peripheral leukocyte through different ways. They are also likely to be related to the embryogenesis and regeneration of several organs and may possess some functions in the central nervous system.     

Effects of SSeCKS on adhesion and migration of bovine pulmonary artery endothelial cells stimulated by fibronectin

AIM: To study the effects of Src suppressed C kinase substrates(SSeCKS) on the adhesion and migration of bovine pulmonary artery endothelial cells (BPAECs) stimulated by fibronectin(FN).METHODS: Cultured BPAEC were stimulated by different concentrations of FN.SSeCKS expressions were detected by Western blotting.Cultured BPAECs were treated with Ro31-8220 and calphostin C (a PKC inhibitor).The locations of SSeCKS,F-actin and vinculin before and after stimulation were observed by confocal microscopy.RESULTS: After stimulated by FN,SSeCKS expression in BPAECs increased in concentration and time dependent manners.After treated with Ro31-8220 and calphostin C,the adhesion and migration of BPVECs were restrained,the expression of SSeCKS was inhibited,SSeCKS distributed from cytosol to perinuclear,and the colocalization of SSeCKS with F-actin,vinculin at the edge of BPAECs was reduced.CONCLUSION: SSeCKS may play an important role in ECs adhesion and migration stimulated by fibronectin.The process can be inhibited by Ro31-8220 and calphostin C.     

Roles of chloride channels in the migration of nasopharyngeal carcinoma cells at different stages of the cell cycle

AIM: To clarify the migration capability of nasopharyngeal carcinoma cells (CNE-2Z) at different stages of the cell cycle and the roles of chloride channels in cell migration. METHODS: Synchronous cells were obtained by the serum deprivation，double chemical-block, mitotic arrest and shake-off techniques. Cell cycle distribution of CNE-2Z cells was analyzed by the flow cytometry. Migration rate was assayed by transwell chambers and by image analysis. The cytotoxicity of chemicals on cells was tested by MTT assay. RESULTS: CNE-2Z cells at different stages of the cell cycle exhibited different migratory ability. The migration rate of the three stages was G1>M> S. The migration of CNE-2Z cells was inhibited by chloride channel blockers (ATP, NPPB and tamoxifen), but the inhibitory effect of the blockers varied with cells at different stages. CONCLUSIONS: The migratory ability is associated with the cell cycle in CNE-2Z cells. Chloride channels play an important role in cell migration of CNE-2Z cells.     

Influence of basic fibroblast growth factor on ECV304 cell migration

AIM: To observe the influence of basic fibroblast growth factor (bFGF) at different concentrations on migration of ECV304 cells after scratch wound. METHODS: Cell migration of ECV304 was monitored by computer assisted video microscopy with an image analysis system after treatment with bFGF at concentrations of 0, 5, 10 and 15 μg/L. The morphological changes induced by bFGF were observed under light microscope and scanning electron microscope. RESULTS: bFGF increased the cell migration at low concentration (5 μg/L), and inhibited at high concentration (15 μg/L). The surface of migration cells exhibited numerous filopodia. CONCLUSION: bFGF has double direction adjustment effects on migration of ECV304 cells. The surface of migration cells exhibits affluent pseudopods, which are mainly filopodia.