Effects of factors of nutrition and husbandry on the heat production of rats and broilers. 1. Heat production of growing broiler chicks kept in groups depending on the environmental temperature

In 2 experiments with young broiler chickens, origin Tetra B, heat production was measured in dependence on ambient temperature indirectly and with the gas exchange both over 24 h and in 20-minute periods beginning on their 5th day of live. The chickens, divided into 2 X 2 groups according to sex, were constantly kept in a climatic chamber changed in to a respiration chamber during the 8- or 11-week-long experiments. The maximum variation of the temperature was between 5 and 35 degrees C. In the periods of 24-h measurements over 4 days each, the ambient temperature was changed from day to day in steps of 5 degrees C. Heat production was influenced by the age of the chickens, energy intake and ambient temperature. The results of the measurements at the same age and the same energy intake and a temperature variation between 5 and 35 degrees C can well be described by polynomes of the 3rd degree up to the 8th week of live. The thermoregulatory conditioned heat production per 1 degree C below the critical temperature decreased with the age of the chickens. In the first few weeks of life it was 20 kJ, in the 6th and 7th weeks of life 10-15 kJ and then decreased to 4-5 kJ/kg life weight 0.75.d. degrees C. Based on the temperature of minimal heat production, the heat production of 16- to 30-day-old chickens increased to 60-80% at an ambient temperature of 5 degrees C; the metabolism of chickens older than 7 weeks was only increased by about 20%. For the first 2 weeks 35 degrees C were ascertained as critical temperature, for the 3rd to 6th weeks 30 degrees C and for the 7th and 8th weeks 25 degrees C.     

Energy metabolism of growing broiler chickens kept in groups in relation to the environmental temperature. 2. Heat production, thermoregulatory heat production and thermoneutral temperature (short term measurement)]

In 5 experiments the weekly rhythm of the heat production of growing young broiler chickens kept in groups between their 5th and 57th days of life was measured according to the method of short-time measuring (30-minute measuring periods) in dependence on environmental temperature (ET) between 40 and 5 degrees C in stages of 5 K by means of gas exchange and indirect calorimetry. The keeping temperature ranged from one experiment to the other from 35, 30, 25, 20 to 15 degrees C. The dependence of heat production on ET is parabolic in the medium and high ranges of temperature. As a rule, heat production increases progressively with very low ET. Thermoneutral temperature depends on both, the age (live weight) of the chickens and environmental temperature. Thermoregulatory heat production decreases with growing age. It changed from 24 kJ/kg LW0.75.d.K at the beginning to 8 kJ/kg LW0.75.d.K at the end of the experiments.     

Energy metabolism of growing broilers in relation to the environmental temperature

7 experiments with 6 chickens each (origin Tetra B) in the live weight range between greater than 100 and less than 300 g and up to 1800 g were carried out at environmental temperatures (ET) of 35, 30, 25 (2 experiments) 20 (2 experiments) and 15 degrees C. In the course of each experiment the chickens alternatively received feed mixtures containing 20 and 40% crude protein (3 animals/variant) for maintenance and weight gain (semi ad libitum). Energy metabolism was measured according to indirect calorimetry over a total of 645 metabolism periods. In the temperature range studied there was no compensation between thermoregulatory heat and heat from other metabolic processes. The partial utilization of metabolizable energy for energy retention in the body was independent of ET and remained in the limits between 71 and 73%. Energy utilization was dependent on the protein content of the feed. It decreased from 75 to 69% with the increase of the protein content from 20 to 40%. Energy requirement for protein retention varied between 1.67 and 1.89 kJ metabolizable energy/kJ and was independent of ET. Energy requirement (metabolizable energy) for the maintenance of the energy balance was independent of the protein content of the feed. It increased from 433 kJ/kg LW0.75.d at 35 degrees C to 693 kJ/kg LW0.75.d at 15 degrees C ET. The relationship between heat production and ET is parabolic. The thermoneutral temperature decreased from 35 to 25 degrees C in the course of development. In the live weight range of 300-500 g thermoregulatory heat production had its maximum with 19 kJ/kg LW0.75.d.K and decreased in the further development to 10-13 kJ/kg LW0.75.d.K.     

Interaction between the temperature of the surroundings, performance and level of feed for growing pigs

Investigations of the performance of growing pigs in the live weight range of 30 to 60 kg kept individually and in groups with ad libitum and rationed feeding were carried out in two air-conditioned rooms. The air temperature in the individual experiments was 20 degrees C, 10 degrees C and 5 degrees C, the relative atmospheric moisture in all experiments was between 70 and 80%. A diminished growth of 24 g per day per degrees C below 20 degrees could be ascertained for pigs kept individually. The diminishing of the growth can be compensated by an increase of the feed intake of 1.3 g per kg live weight, day and degrees C resp. the intake of metabolisable energy of 4 kcal per kg live weight, day and degrees C until the capacity of feed consumption is reached.     

Consequences of nutritional and husbandry factors on the heat production of rats and broilers. 2. Effect of environmental temperature on the heat production of fully grown broilers and rats

In an experiment with broilers (origin Tetra B) and with rats (albino, Wistar line) with 2 animals each, heat production was ascertained by measuring CO2 production and O2 consumption over 20 minutes after their feeding 18 h and 1 h before the beginning of measuring at ambient temperatures of 30, 25, 20, 15 and 10 degrees C. Every variant was followed through over 6 h/d in 12 measuring sections. The feed amount/ánimal and day was adapted to energy maintenance requirement. At the beginning of the experiments the broilers and rats were 14 and 21 weeks old resp. and weighed 2.2 kg and 220 g resp. The variation of the ambient temperature did not influence the heat production of the broilers. In contrast to this, the time of feeding in relation to the beginning of measuring had a distinct effect on heat production. Whereas a heat production of 342 +/- 34 kJ/kg LW0.75.d was ascertained in the postabsorptive state 18 h after the last feed intake, it increased by 11% to 393 +/- 32 kJ/kg LW0.75.d when measuring began 1 h after feeding. The very act of feed intake increased heat production by 75%. Rats showed a distinct increase of heat production caused by a decreasing ambient temperature. In the temperature range of 30-25 degrees C the increase was shallower than in the range of 25-10 degrees C. Per 1 degrees C below 25 degrees C heat production increased by 30 kJ/kg LW0.75.d. The increase was independent of the metabolism level, which was influenced by the feeding variants. The results are discussed in connection with Rubner's theory of heat compensation.     

Energy metabolism of growing rats in relation to the environmental temperature

8 experiments were carried out with 9 albino rats each (Wistar line, bred at the institute) in the live weight range between 70 and 200 g and at environmental temperatures (ET) of 34, 32, 30, 28, 26, 24, 22 and 20 degrees C. In the course of each individual experiment the rats were alternatively fed for maintenance and weight gain (semi ad libitum) with feed mixtures containing 10, 25 and 40% crude protein (3 animals/variant). Energy metabolism was measured according to the method of indirect calorimetry over a total of 780 metabolism periods. In the temperature range studied there was no compensation between thermoregulatory heat and heat from other processes of the metabolism. The partial utilization of metabolizable energy for energy retention in the body was independent of ET and ranged between 73 and 80% for the 7 experiments with ET between 32 and 20 degrees C. Energy utilization depended on the protein content of the feed and decreased from 81 to 79 or 73 resp. when the protein content increased from 10 to 25% or to 40% resp. Energy requirement for protein retention varied between 1.61 and 2.09 kJ metabolizable energy/kJ and was independent of ET. Energy maintenance requirement (measured at 28, 30 and 32 degrees C) increased with the growing protein content from 415 to 439 and 447 kJ/kg LW0.75.d resp. (regression analysis) and from 411 to 420 and 432 kJ/kg LW0.75.d (measuring at maintenance level). The relative weight gain with the increased protein content of the feed largely corresponds to the expected values according to the efficiency of ATP synthesis in the oxidative degradation of nutrients. The relationship between heat production and ET is parabolic. In the live weight range studied the average thermoneutral temperature (TNT) was 32 degrees C. It decreased during the course of development from 34 to 30 degrees C. TNT decreased with the growing protein content of the feed. Thermoregulatory heat production depended on both environmental temperature and the stage of development. Its average value in the development range studied decreased with an increase of the environmental temperature by 2 K each, starting from 20 degrees C and rising to 32 degrees C, in the following linear sequence: 23.3, 21.0, 16.8, 12.5, 8.3, 4.0 and 0.3 kJ/kg LW0.75.d.K.     

Influence of high ambient temperatures on performance of multiparous lactating sows.

Multiparous Large White sows (n = 63) were used to investigate the effects of five ambient temperatures (18, 22, 25, 27, and 29 degrees C) and two dietary protein contents on their lactation performance. At each temperature treatment, ambient temperature was maintained constant over the 21-d lactation period. Dietary protein content was either 14 or 17% with essential amino acids levels calculated not to be limiting. The animals had ad libitum access to feed between the seventh and the 19th day of lactation. Diet composition did not influence lactation performance. Over the 21-d lactation, feed intake decreased from 5.67 to 3.08 kg/d between 18 and 29 degrees C. Between d 7 and 19, the corresponding values were 7.16 and 3.48 kg/d, respectively. This decrease was curvilinear; an equation to predict voluntary feed intake (VFI) from temperature (T, degrees C) and body weight (BW, kg) is proposed: VFI = -49,052 + 1,213 T - 31.5 T2 + 330 BW - .61 BW2 (residual standard deviation: 1,018). Skin temperature increased regularly with increased ambient temperature (34.6 to 37.4 degrees C between 18 and 29 degrees C), whereas udder temperature reached a plateau at 25 degrees C (38.3 degrees C). The gradient of temperature between skin and rectum was minimal (2 degrees C ) at 27 degrees C and remained constant at 29 degrees C. This constancy coincides with the marked reduction of feed intake. The respiratory rate increased from 26 to 124 breaths/min between 18 and 29 degrees C, and this indicates that the evaporative critical temperature was below 22 degrees C. The BW loss increased from 23 to 35 kg between 18 and 29 degrees C, but its estimated chemical composition remained constant. Pig growth rate was almost constant between 18 and 25 degrees C (241 g/d) and was reduced above 25 degrees C (212 and 189 g/d at 27 and 29 degrees C, respectively). In conclusion, temperatures above 25 degrees C seem to be critical for lactating sows in order to maintain their performance.     

Rearing of female cattle twins using complete feed pellets in comparison to straw pellets plus concentrate mixture. 1. Weight gain performance and feed expenditure as well as rumen fermentation conditions during the 4th-12th months of life

The investigations with young female cattle (twin cattle, n = 30) the applicability of straw as sole roughage after the end of the suckling period was tested. Completely (I) and partly (II) pelleted rations of dried feed with a straw component of 65% each in the total rations were used. Feed intake, development of body weight, feed expenditure as well as indexes of rumen fermentation and of the concentration of some metabolism parameters in the blood serum were measured. In the complete test period (between 5th and 12th months of life, 243 days) there were not any significant differences between the groups in all registrations. Average daily weight gains of 570 g were achieved with an overall high dry matter (2.6 kg/100 kg live weight) and roughage (straw) intake (1.6 kg dry matter/100 kg live weight). The standard expenditure of energy and protein given for the stage of development was differentiatedly undercut. The energy expenditure being below the standard (ca. 10%), the total energy expenditure (kEFUcattle) was by 20 (II) and 25% (I) below standard expenditure. Energy expenditure aimed at weight gain reached very satisfactory values with 3.68 (I) and 3.79 kEFUcattle/kg weight gain (II). Protein expenditure fell short of the standard for the development stage by 14 (II) and 20% (I) resp. All parameters ascertained of the rumen and intermediate metabolism were in the physiologic standard range, as is characteristic of feeding regimes with a relatively high quota of roughage.     

The maintenance requirement of domestic drakes

1. Mature domestic drakes of 7 genotypes, ranging in live weight from 1.1 to 5.1 kg, were each given a daily allowance of feed just below the level of recorded ad libitum intake. 2. House temperature was maintained at 26 degrees C for 16 weeks and then at 10 degrees C for a further 8 weeks. 3. Under these conditions, live weight quickly adjusted to the level of feed supplied and then remained stable. 4. Regression of metabolisable energy intake on live weight (W) yielded estimates of maintenance requirement of 583 kJ/kgW(0.75).d at 10 degrees C and 523 kJ/kgW(0.75).d at 26 degrees C.     

Methodologic studies on protein metabolism and bioenergetics of protein deposition in growing animals. 4. Energy metabolism in chickens in connection with measurement of parameters of protein metabolism

In connection with the measuring of parameters of the protein metabolism in parallel experiments, the energy metabolism of 6 chickens (origin Tetra B) in the live weight range between approximately 100 and 1,800 g was determined under conditions of restricted energy supply. 3 animals each received a feed mixture containing 20% (animal group 1) and 38% (animal group 2) crude protein. The amount of feed was daily increased by 1.5 g DM. The digestibility of energy and nitrogen was independent of the age. 66.3 +/- 3.3% and 64.0 +/- 5.0% resp. of the metabolisable energy were utilised for protein and fat retention. The energy maintenance requirement, determined at a live weight of 2,000 g, was independent of protein supply and averaged in the two animal groups 434 +/- 40 kJ metabolisable energy/kg live weight 0.75 . d. The result of multiple regression was, for the growth period investigated, an energy maintenance requirement of 403 +/- 32 kJ metabolisable energy/kg live weight 0.75 . d. 1.77 and 1.38 J metabolisable energy resp. were required for 1 J protein or fat retention. The energy requirement for protein retention was independent of the degree of protein supply. The results from the measuring of energy metabolism are discussed in connection with the kinetic parameters of protein metabolism ascertained in parallel experiments.     

Continuous measuring of heat production during the course of the day in fully grown rats at different nutrition levels and different environmental temperatures

The heat production of 4 rats was measured by means of indirect calorimetry over 20 h/d at intervals of 4 min at ambient temperatures of 30, 25, 20 and 15 degrees C and feed intakes of 0, 4, 8 and 12 g/d. When the rats were hungry, their heat production was reduced by between 8 and 44 kJ/kg LW0.75.d. Feed intake increased heat production by between 54 and 102%. In the temperature range between 20 and 30 degrees C the rats required 0.36 kJ or 2.4% resp. of the metabolizable energy for the intake of 1 g feed. At 15 degrees C the corresponding values were 0.48 kJ or 3.2% resp. The activity-conditioned quota of heat production was estimated as 31 +/- 10%. In the temperature range of between 30 and 25 degrees C thermoregulatory heat production amounted to 5 and that in the temperature range between 25 and 15 degrees C to 20 kJ/kg LW0.75.d.K. No compensation of thermoregulatory heat by heat from increase of energy intake could be proved in the temperature range between 15 and 30 degrees C.     

Effects of ambient temperature on heat increment of feeding and energy retention in growing broilers maintained at different food intakes

Zero-activity heat production (HP), body temperature (Tb) and energy retention were measured in growing broilers maintained at 5 ambient temperatures (Ta) (14 degrees , 17 degrees , 22 degrees , 27 degrees and 32 degrees C) and at 5 feeding rates (ad libitum intake and 75%, 50%, 25% and 0% (fasting) of ad libitum). Zero-activity HP increased with decreasing Ta and increasing food intake. However, at 14 degrees C, zero-activity HP in birds fed ad libitum and 75% did not show further increase, but those in birds fed less than 75% of ad libitum increased rapidly. Results of the regression of zero-activity HP on Ta ranging from 32 degrees to 17 degrees C indicated that the slope was affected little by food intake, but the intercept decreased with decreasing food intake. Tb increased significantly with increasing food intake. There was little variation with Ta but, at and above 27 degrees C, a slightly increased Tb was observed only in birds fed ad libitum. Overall effects of Ta and food intake on HIF (% TME intake) were not found, but HIF tended to increase with decreasing food intake at 14 degrees C. Total energy retention and energy retention as fat decreased with decreasing Ta and food intake, although energy retention as protein decreased only with decreasing food intake. Results obtained here suggest that availability of TME is affected little by Ta ranging from 32 degrees to 17 degrees C and that HIF is utilised, in part, to maintain Tb at any Ta.     

The rearing of female twin cattle using only feed pellets in comparison with straw pellets plus a concentrate mixture. 2. Weight gain and feed consumption as well as rumen fermentation in the 1st half of the 2d year of life

In investigations with 30 young cows (twin cattle) the applicability of straw materials as sole roughage was tested during the first half of their second year of life (183 feeding days). In the course of the experiment sole feed pellets (I) and partly pelleted rations of dried feed (straw pellets plus loose wheat and loose wheat straw resp.; II) were used. Approximately 72% of the dry matter intake was provided from straw materials in both groups and on an average of the test period. Weight gain and feed expenditure were ascertained and at the end of the test period also the parameters of rumen fermentation and of the concentration of some metabolism parameters in the blood serum. With feed supply being restrictive (2.2 kg dry matter/100 kg live weight), a high dry matter intake from straw (1.6 kg/100 kg live weight or 4.8 kg/animal and day resp.) and average weight gains of between 574 (I) and 582 g (II) were achieved. The standard expenditure of energy (kEFUcattle) and protein stated in the GDR feed evaluation system for this period of development was undercut by 27 (II) and 29 (I)% and 12 (II) and 18 (I)% resp. On average the expenditure of kEFUcattle/kg weight gain was 4.75 (I) and 4.98 (II) and that of digestible crude protein/kg weight gain 679 (I) and 733 g (II). Compared to standard values the expenditure of energy and feed expenditure from concentrates was 80%. Rumen fermentation conditions corresponded to those of a high cellulolytic activity (C2: C3 ratio of greater than 4: 1). The only significant differences between the groups at the time of sampling were to be observed in the total concentration of volatile fatty acids and in the pH-value in the rumen. The absolute (1/animal) and relative (1/kg dry matter intake) volume of rumen fluid varied between 65 and 70 and between 10.5 and 12.5 1. The ascertained concentration of selected metabolism parameters were within the physiologic standard range.     

Growth studies in bulls of the black-and-white dairy cattle breed at a low feeding level. 1. Growth progress and fattening performance]

In 2 experiments with a total of 108 black-and-white dairy bulls feed intake, growth performance and energy expenditure for live weight and carcass production with low feeding intensity were ascertained. The performance level varied between 600 and 650 g; 24-month-old bulls achieved a final weight of 458 and 517 kg resp. Growth and retention performance were considerably influenced by the energy concentration of the ration and by daily energy intake.     

Effects of the addition of roller mill ground corn to pelleted feed during a fifty-six-day production period on growth performance and processing yields of broiler chickens

Poor pelleting production rates can raise the milling cost and increase the frequency of feed outages for broiler integrated operations. The number of broilers marketed to heavy BW has been increasing, and meeting feed delivery schedules can be problematic with “big bird” complexes. Adding rolled corn post pellet is a management strategy to increase feed production rate while concurrently decreasing energy use of the grinding and pelleting processes. This study evaluated the addition of screened rolled corn to pelleted feed on live performance and meat yield of broiler chickens during a 56-d grow-out. Four dietary treatments were provided: A) a control, no added corn post pellet from 18 to 56 d of age; B) addition of 25% of the formulated corn post pellet from 18 to 56 d of age; C) addition of 10, 20, and 30% of the formulated corn post pellet from 18 to 35, 36 to 46, and 47 to 56 d of age, respectively; and D) addition of 20, 30, and 40% of the formulated corn post pellet from 18 to 35, 36 to 46, and 47 to 56 d of age, respectively. Final BW, cumulative feed conversion, 38-d BW uniformity, and 57-d breast meat weight and yield were similar among the dietary treatments. These results indicate screened rolled corn can be added post pellet without adversely affecting broiler performance and meat yield.     

Sensorial quality and bone strength of female and male broiler chickens are influenced by weight and growth rate

1. An experiment was conducted with 98 male and 98 female broiler chickens (Ross 308) to study the effect of growth rate, induced by different dietary means, sex and live weight (1500?g and 2000?g) at slaughter on production parameters, bone strength and sensorial characteristics of the breast meat.

2. The birds were divided into four groups and individually fed a standard commercial diet, a high energy diet or low energy diet from d?11 to slaughter at between d 28 and 39. Three groups were fed ad libitum and a further group was fed a restricted amount of the high energy feed. Half of the birds in each group were slaughtered at approximately 1500?g and the other half at 2000?g live weight.

3. The diets resulted in different growth rates. The chickens fed the high energy and the commercial diet had the highest growth rate at both live weights at slaughter. The restricted fed chickens had lower bone strength than the chickens fed the low energy diet.

4. Breast meat from male broilers was juicer, more tender and less hard than breast meat from females. Chickens slaughtered at 2000?g live weight were juicer than those slaughtered at 1500?g. Chickens given the high energy feed ad libitum and restricted had different growth rates, but the sensory parameter related to texture showed no difference.

5. It was concluded that an increased slaughter weight might improve meat quality due to improved juiciness.     

Nutrient dependence of energy conservation requirements in rats. 4. The influence of protein levels of food on energy conservation requirements of rats during growth and after conclusion of the intensive growth phase

Albino rats bred in the institute (Wistar line) divided into 3 groups of 9 animals each received, beginning at the age of 4 weeks, feed mixtures with 10, 40 and 70% protein in the rations over a period of 24 weeks divided into 14 subperiods of study. The feed mixture changed cyclically for the groups of animals after each sub-period. Every period was divided into a growth period (8 days) with the rats kept in metabolism cages and a period of feeding on maintenance level (4 days) with the rats kept in respiration chambers. In both periods the temperature was kept constant at 30 degrees C. On 3 days of feeding on the maintenance level the metabolism parameters of energy, C and N metabolism were measured and energy maintenance requirement was ascertained. Both the energy maintenance requirement of the growing rats (up to 200 g live weight) and that of the nearly fully grown and fully grown rats resp. (greater than 200 g live weight) significantly depended on the nutrient composition of the feed mixtures supplied. It increased with the increasing protein and simultaneously decreasing carbohydrate quotas in the feed. On an average of the studies the rats had, in the sequence of 10, 40 and 70% protein content, an energy maintenance requirement of 383 +/- 31 (n = 105), 415 +/- 31 (n = 106) and 459 +/- 36 kJ metabolizable energy/kg LW0.75.d (n = 102). Energy maintenance requirement behaved relatively like 100:108:120. Based on the fact that energy maintenance requirement may be considered the requirement of ATP, relative expectancy values for energy maintenance requirement can be calculated with the energetic efficiency of the ATP synthesis (kJ metabolizable energy/Mol ATP gain) in nutrient catabolism from the relation of the experimentally ascertained nutrient metabolism at a variant supply of protein of 100:110:118. The hypothesis that the efficiency of ATP synthesis in the catabolism of the main nutrients supplying energy can be considered a relative measure of the dependence of the energy maintenance requirement on nutrient composition has been confirmed in this experiment. Different findings in earlier experiments raise the question if those findings were influenced by adaptation effects. An experimental solution of this question is considered important.     

Performance of growing rabbits fed concentrate and Stylosanthes (Verano) combinations under tropical conditions

The performance of growing crossbred rabbits fed concentrate and Stylosanthes hamata (stylo) at different ratios was evaluated. The following ratios were used: 25:100, 50:75, 75:50, 100:25 and 125:10 (g : g; weight supplied per day). Total weight gain was lower and the feed : gain ratio (FGR) was higher with the 25:100 combination than with the other combinations. Daily feed intake significantly (P < 0.01) decreased, whereas feed cost and cost per kg intake increased as the concentrate level increased. Cost per kg gain increased with increases in concentrate levels up to 100 g before decreasing at 125 g. Weight gain, cost per kg intake and cost per kg gain had a quadratic relationship with concentrate level, whereas FGR and feed cost had a linear relationship. Time required to attain a market weight of 2.5 kg and total feed required to attain market weight decreased, whereas total cost of feeding increased with increases in concentrate level. Dry matter and ash digestibility were similar for all the treatments. Ether extract digestibility was lower for the 25:100 combination compared with the other treatments. Crude protein, nitrogen‐free extract digestibility and nitrogen retention were significantly lower for the 25:100 combination (0.74, 0.66 and 0.64) compared with the 100:25 combination (0.83, 0.75 and 0.79). The dressing percentage was similar for all treatments, whereas the proportion of live weight represented by the head increased with an increase in concentrate level. This study shows that rabbits are able to utilize leaf protein in stylo for growth even at the lowest level of concentrate.     

Effect of dietary ascorbic acid supplementation level on productivity,mortality, and carcass characteristics of Venda chickens

Two experiments were carried out to determine the effect of dietary ascorbic acid supplementation levels on productivity, carcass characteristics, and mortality of indigenous Venda chickens. The first experiment determined the effect of dietary ascorbic acid supplementation levels on productivity and mortality rate of 175 unsexed Venda chickens between 1 and 6 weeks old. The second experiment determined the effect of dietary ascorbic acid supplementation levels on productivity, carcass characteristics, and mortality rate of 140 female Venda chickens between 8 and 13 weeks old. A completely randomized design was used in both experiments. Supplementation of grower diets with ascorbic acid ranged from 0 to 2,000 mg per kg DM feed in both experiments. Levels of ascorbic acid supplementation for optimum feed intake, feed conversion ratio, growth rate, live weight, and breast meat yield were determined using a quadratic equation. The optimal dietary ascorbic acid supplementation levels for feed conversion ratio, growth rate, and live weight of Venda chickens during the starter phase were 1,050, 1,301, and 1,500 mg/kg DM feed, while, at the grower phase, the optimal supplementation levels for feed conversion ratio, growth rate, live weight, and breast meat yield were 1,000, 1,250, 1,482, and 769 mg/kg DM feed, respectively. Results indicate that different levels of ascorbic acid supplementation optimized feed conversion ratio, growth rate, and live weight of Venda chickens at each growth phase. However, levels of ascorbic acid supplementation for optimum feed conversion ratio, growth rate, and live weight were higher than that for breast meat yield. These findings have implications on ration formulation for Venda chickens.     

Energy and protein concentration of broiler rations in Cuba. 4. Female broilers in the summer

A total of 1,900 female broilers of the variety White Plymouth Rock was tested with 4 energy concentrations (10.5, 11.5, 12.6 and 13.6 MJ metabolizable energy/kg feed) and 4 protein concentrations (15.0, 17.5, 20.0 and 22.5% CP) in their first 56 days of life in 16 test groups. The animals were kept in cages and were directly exposed to the natural Cuban summer temperatures from their 15th day of life onwards. As every test group was run with 6 repetitions, the results could be variance analytically calculated. In a comparison of male and female broilers in summer one can state that the female animals achieved a lower intake of feed, energy and crude protein as well as a lower live weight than the male animals. In winter the female broilers consumed between 15.7 and 36.4% more feed and achieved a live weight gain between 11.1 and 43% higher than in summer. It could be shown that the maximum live weight of the female broilers could be achieved in summer if rations containing 12.6 MJ metabolizable energy and 20% CP are fed in a one-phase feeding system.