印度热带森林干旱扰动土壤的微生物碳, 氮, 磷的研究

Variations in microbial biomass C (MB-C),N (MB-N) and P (MB-P) along a gradient of different dominant vegeta- tion covers (natural forest,mixed deciduous forest,disturbed savanna and grassland ecosystems) in dry tropical soils of Vindhyan Plateau,India were studied from January 2005 to December 2005.The water holding capacity,organic C,total N,total P and soil moisture content were comparatively higher in forest soils than in the savanna and grassland sites.Across different study sites the mean annual MB-C,MB-N and MB-P at 0-15 cm soil depth varied from 312.05 ± 4.22 to 653.40 ± 3.17,32.16 ± 6.25 to 75.66 ± 7.21 and 18.94 ± 2.94 to 30.83 ± 23.08 μg g ?1 dry soil,respectively.At all the investigated sites,the maximum MB-C,MB-N and MB-P occurred during the dry period (summer season) and the minimum in wet period (rainy season).In the present study,soil MB-C,MB-N and MB-P were higher at the forest sites compared to savanna and grassland sites.The differences in MB-C,MB-N and MB-P were significant (P 0.001) among sites and seasons.The MB-C (P 0.0001),MB-N (P 0.001) and MB-P (P 0.0001) were positively correlated with organic C,while the relationship between soil moisture and MB-C,MB-N and MB-P (P 0.001,P 0.01 and P 0.0001,respectively) was negative.The decreasing order of MB-C,MB-N and MB-P along study ecosystems was natural forest mixed deciduous forest savanna grassland.The results suggested that deforestation and land use practices (conversion of forest into savanna and grassland) caused the alterations in soil properties,which as a consequence,led to reduction in soil nutrients and MB-C,MB-N and MB-P in the soil of disturbed sites (grassland and savanna) compared to undisturbed forest ecosystems.     

中国南亚热带三种不同林型下土壤应对酸沉降的响应

Long-term changes in soil pH, the current status of soil acidification, and the response of bulk soil and soil water pH to experimental nitrogen addition under three subtropical forests were investigated in Dinghushan Biosphere Reserve of subtropical China. The results showed that the mineral soil pH at 0-20 cm depth declined significantly from 4.60-4.75 in 1980s to 3.84-4.02 in 2005. Nitrogen addition resulted in the decrease of pH in both bulk soil and soil water collected at 20-cm depth. The rapid decline of soil pH was attributed to long-term high atmospheric acid deposition (nitrogen and sulphur) therein. The forest at earlier succession stage with originally higher soil pH appeared to be more vulnerable to acid deposition than that at later succession stage with originally low soil pH.     

南亚热带三种典型植被土壤动物对模拟N沉降增加的早期响应

A field-scale experiment arranged in a complete randomized block design with three N addition treatments including a control （no addition of N）, a low N （5 g m^-2 year^-1）, and a medium N （10 g m^-2 year^-1） was performed in each of the three typical forests, a pine （Pinus massoniana Lamb.） forest （PF）, a pine-broadleaf mixed forest （MF） and a mature monsoon evergreen broadleaf forest （MEBF）, of the Dinghushan Biosphere Reserve in subtropical China to study the response of soil fauna community to additions of N. Higher NH4^＋ and NO3^- concentrations and a lower soil pH occurred in the medium N treatment of MEBF, whereas the NO3^- concentration was the lowest in PF after the additions of N. The response of the density, group abundance and diversity index of soil fauna to addition of N varied with the forest type, and all these variables decreased with increasing N under MEBF but the trend was opposite under PF. The N treatments had no significant effects on these variables under MF. Compared with the control plots, the medium N treatment had significant negative effect on soil fauna under MEBF. The group abundance of soil fauna increased significantly with additions of higher N rates under PF. These results suggested that the response of soil fauna to N deposition varied with the forest type and N deposition rate, and soil N status is one of the important factors affecting the response of soil fauna to N deposition.     

不同土地利用方式下土壤微生物群落水平生理图谱(CLP)与土壤碳、氮有效性之间的相互关系

The goal of this work was to assess soil microbial respiration, determined by the assay of community-level physiological profiling in an oxygen-sensitive microplate (O₂-CLPP), in response to endogenous C and several individual C substrates in the soils with different organic C contents (as a function of soil type and management practice). We also used the O₂-CLPP to determine the respiratory response of these soils to endogenous C and amended C substrates with N addition. A respiratory quotient (RQ) was calculated based on the ratio of the response to endogenous soil C vs. each C-only substrate, and was related to total organic carbon (TOC). For assessing N availability for microbial activity, the effect of N supplementation on soil respiration, expressed as N_ratio, was calculated based on the response of several substrates to N addition relative to the response without N. Soils clustered in 4 groups after a principal component analysis (PCA), based on TOC and their respiratory responses to substrates and endogenous C. These groups reflected differences among soils in their geographic origin, land use and C content. Calculated RQ values were significantly lower in natural forest soils than in managed soils for most C-only substrates. TOC was negatively correlated with RQ (r = - 0.65), indicating that the soils with higher organic matter content increased respiratory efficiency. The N addition in the assay in the absence of C amendment (i.e., only endogenous soil C present) had no effect on microbial respiration in any soil, indicating that these soils were not intrinsically N-limited, but substrate-dependent variation in N_ratio within soil groups was observed.     

不同土地利用方式土壤温室气体排放对碳氮添加的响应

揭示不同土地利用方式下土壤N2O产生机制及其CO2和CH4的排放,有助于土壤温室气体减排措施的制定。本研究以长沙金井河流域酸性红壤上菜地、稻田、茶园和林地土壤为研究对象,控制温度和土壤含水量,采用静态培养-气相色谱法,研究4种利用方式土壤N2O、CO2和CH4的排放对不同碳氮和硝化抑制剂添加的响应。结果表明,由于土壤pH较低,酸性红壤外加氮源后仅有较小的N2O排放。葡萄糖能够促进尿素添加后N2O的排放及土壤反硝化作用N2O的排放。异养硝化作用可能是酸性红壤N2O产生的主要途径。硝化抑制剂双氰胺（DCD）对酸性红壤N2O减排无明显效果。碳氮添加后土壤N2O的总排放量表现为茶园 > 菜地 > 稻田 > 林地。外源有机碳能够显著促进4种利用方式土壤CO2的排放,表现为茶园、稻田 > 菜地、林地。但除稻田土壤CH4排放增加外,菜地、茶园和林地土壤CH4排放对外源有机碳无明显响应。     

氮肥用量及其分施比例对棉花氮利用和土壤氮平衡的影响

The Yellow River valley is one of the three largest cotton production areas in China.An experiment was performed in cotton fields of Anyang,China from 2013 to 2014 to investigate the effects of nitrogen(N) application rate and the ratio between basal and topdressing N fertilizer on N balance in a soil-plant system,N use efficiency,and cotton yield.Five N application rates as treatments were applied with the same split application ratio.Half of the N(50% basal fertilizer) was applied at pre-planting and the other half(50% topdressing fertilizer) at the initial flowering stage.These treatments were:zero N(N0,control),90 kg N ha~(-1)(N90(5/5)),180 kg N ha~(-1)(N180(5/5)),270 kg N ha~(-1)(N270(5/5),a reduced N rate),and 360 kg N ha~(-1)(N360(5/5),a conventional N rate).Additional 2 split application ratios as treatments were applied with the same N rate of 270 kg N ha~(-1).The split application ratios between basal N and topdressing N were 30%:70%(N270(3/7)) and 70%:30%(N270(7/3)).Results demonstrated that soil NH_4-N content in the 0–60 cm layer and NO3-N content in the 0–20 cm layer increased with increased N rate at the squaring and boll-opening stages and then decreased to lower levels at the initial flowering and harvest stages.Soil NO_3-N content in the 20–60 cm layer after the initial flowering stage increased with the increase of topdressing N rate.Soil apparent N surplus varied at different growth stages,while the soil apparent N surplus over the entire growth period exhibited a positive relationship at N rates over 180 kg ha~(-1).Seed cotton yield of N270(3/7) was the highest of all treatments.Plant N uptake,N agronomic efficiency,and apparent N recovery efficiency of N270(3/7) were significantly higher than those of N270(5/5) and N270(7/3) in both growing seasons.These suggest both economic and ecological benefits in cotton production in the Yellow River valley could be created,by appropriately reducing total N application rate and increasing the ratio of topdressing to basal N fertilizer at the initial flowering stage.     

土壤碳氮对多年生能源草、覆盖作物和氮施肥的响应

Cover crop and nitrogen(N) fertilization may maintain soil organic matter under bioenergy perennial grass where removal of aboveground biomass for feedstock to produce cellulosic ethanol can reduce soil quality. We evaluated the effects of cover crops and N fertilization rates on soil organic carbon(C)(SOC), total N(STN), ammonium N(NH_4-N), and nitrate N(NO_3-N) contents at the0–5, 5–15, and 15–30 cm depths under perennial bioenergy grass from 2010 to 2014 in the southeastern USA. Treatments included unbalanced combinations of perennial bioenergy grass, energy cane(Saccharum spontaneum L.) or elephant grass(Pennisetum purpureum Schumach.), cover crop, crimson clover(Trifolium incarnatum L.), and N fertilization rates(0, 100, and 200 kg N ha~(-1)). Cover crop biomass and C and N contents were greater in the treatment of energy cane with cover crop and 100 kg N ha~(-1) than in the treatment of energy cane and elephant grass. The SOC and STN contents at 0–5 and 5–15 cm were 9%–20% greater in the treatments of elephant grass with cover crop and with or without 100 kg N ha~(-1)than in most of the other treatments. The soil NO_3-N content at 0–5 cm was 31%–45% greater in the treatment of energy cane with cover crop and 100 kg N ha~(-1)than in most of the other treatments.The SOC sequestration increased from 0.1 to 1.0 Mg C ha~(-1)year~(-1)and the STN sequestration from 0.03 to 0.11 Mg N ha~(-1)year~(-1)from 2010 to 2014 for various treatments and depths. In contrast, the soil NH_4-N and NO_3-N contents varied among treatments,depths, and years. Soil C and N storages can be enriched and residual NO_3-N content can be reduced by using elephant grass with cover crop and with or without N fertilization at a moderate rate.     

模拟氮沉降对温带不同森林类型土壤氮矿化速率的影响

通过室内模拟不同氮形态(NH4+-N、NO3--N、NH4+-N+NO3--N)沉降实验,研究不同氮形态沉降对温带不同森林类型(椴树红松混交林、白桦天然次生林、红松人工林和落叶松人工林)土壤氮矿化速率的影响。结果表明:在整个培养期间,与对照相比,经过氮沉降土壤净氨化速率、净硝化速率及净矿化速率都呈现出增长趋势,而其增加的程度又取决于森林类型、土层、氮处理类型和处理时间。不同林型土壤净氨化速率、净硝化速率及净矿化速率受氮沉降影响不同,混交林对氮沉降的响应要弱于阔叶林,高于针叶人工纯林;土壤A层比土壤B层对氮沉降敏感;以铵态氮形态沉降时对铵态氮含量、净氨化速率影响较大,以硝态氮形态沉降时对硝态氮含量、净硝化速率影响较大,混合形态的氮沉降要比单种形态的氮沉降使土壤净氨化速率、净硝化速率及净矿化速率增加幅度更高;氮沉降时间越长,土壤净氨化速率、净硝化速率及净矿化速率与对照差距越大,说明氮沉降对土壤的影响存在累加效应。     

黄土高原天然草地3种优势物种细根分解及养分释放对模拟氮沉降的响应

细根分解是草地土壤有机质和养分的主要来源,全球N沉降背景下细根分解动态变化对生态系统碳和养分循环具有重要意义。采用埋袋法研究黄土高原天然草地3个优势物种细根分解速率和养分释放规律及其对模拟N沉降(10g·N/(m2·a))的响应。结果表明:细根分解过程分为快速(0~60d)和慢速分解(60~719d)2个阶段。大针茅、甘青针茅和白莲蒿细根分解60d的质量残留率分别为86.3%,86.2%,90.7%,分解719d的质量残留率分别为58.1%,64.7%,70.5%,表明细根分解速率大小为大针茅甘青针茅白莲蒿。相关分析表明,细根分解常数与细根初始N含量、N/P值呈显著的正相关关系,与初始C含量、C/N值呈显著的负相关关系。随着根系分解,3种细根C元素表现为直接释放,N元素表现为N富集,P元素表现为富集-释放模式,整体呈波动式下降。模拟N沉降显著抑制了3种植物细根的分解,使大针茅、甘青针茅和白莲蒿细根分解系数分别显著降低了21.3%,26.8%,47.4%。模拟N沉降使大针茅、甘青针茅和白莲蒿分解末期C元素分别增加了26.8%,20.7%,16.6%,N元素分别增加了18.2%,17.0%,13.4%,而对P残留率均没有显著影响。综上,未来氮沉降的增加会抑制黄土高原封育草地优势物种的细根分解速率,减慢其细根分解向土壤输入C和N养分的过程。     

模拟氮沉降对亚热带竹林不同凋落物组分分解的影响

As an important component of the global carbon (C) budget, litter decomposition in terrestrial ecosystems is greatly affected by the increasing nitrogen (N) deposition observed globally. We hypothesized that different litter fractions derived from a single tree species may respond to N deposition differently depending on the quality of the litter substrate. To test the hypothesis, a two-year field experiment was conducted using the litterbag method in a Pleioblastus amarus plantation in the rainy region of Southwest China. Four N treatment levels were applied: control (no N added), low-N (50 kg N ha-1 year-1), medium-N (150 kg N ha-1 year-1), and high-N (300 kg N ha-1 year-1). We observed different patterns of mass loss for the three P. amarus litter fractions (leaves, sheaths, and twigs) of varying substrate quality in the control plots. There were two decomposition stages with different decay rates (fast rate in early stages and slow rate in the later stages) for leaves and sheaths, while we did not observe a slower phase for the decay of twigs during the 2-year study period. The annual decomposition rate (k) of twigs was significantly lower than that of leaves or sheaths. Addition of N slowed the decomposition of leaves and twigs in the later stages of decomposition by inhibiting the decay of lignin and cellulose, while addition of N did not affect the mass loss of sheaths during the study period. In the decomposition of all three litter fractions, experimental N deposition reduced the net N accumulation in the early stages and also decreased the net N release in the later stages. The results of this study suggest that litter substrate quality may be an important factor affecting litter decomposition in a bamboo ecosystem affected by N deposition.     

Effect of Simulated N Deposition on Soil Exchangeable Cations in Three Forest Types of Subtropical China

The effects of simulated nitrogen (N) deposition on soil exchangeable cations were studied in three forest types of subtropical China.Four N treatments with three replications were designed for the monsoon evergreen broadleaf forest (mature forest):control (0 kg N ha-1 year-1),low N (50 kg N ha-1 year-1),medium N (100 kg N ha-1 year-1) and high N (150 kg N ha-1 ycar-1),and only three treatments (i.e.,control,low N,medium N) were established for the pine and mixed forests.Nitrogen had been applied continuously for 26 months before the measurement.The mature forest responded more rapidly and intensively to N additions than the pine and mixed forests,and exhibited some significant negative symptoms,e.g.,soil acidification,Al mobilization and leaching of base cations from soil.The pine and mixed forests responded slowly to N additions and exhibited no significant response of soil cations.Response of soil exchangeable cations to N deposition varied in the forests of subtropical China,depending on soil N status and land-nse history.     

Ammonium nitrate as extractant for soil exchangeable cations,exchangeable acidity and aluminum

Abstract

NH₄NO₃ (1 M) has been used as an extractant for soil exchangeable cations, exchangeable acidity and aluminum. The results obtained using NH₄NO₃ were identical to those using KC1 and NH₄Cl as extractants for the purpose of evaluating soil chemical status. The NH₄NO₃ extraction has practical analytical advantages.     

Effects of nitrogen deposition on soil organic carbon fractions in the subtropical forest ecosystems of S China

Experiments were conducted between 2003 and 2008 to examine how N additions influence soil organic C (SOC) and its fractions in forests at different succession stages in the subtropical China. The succession stages included pine forest, pine and broadleaf mixed forest, and old‐growth monsoon evergreen broadleaf forest. Three levels of N (NH₄NO₃)‐addition treatments comprising control, low‐N (50 kg N ha^–1 y^–1), and medium‐N (100 kg N ha^–1 y^–1) were established. An additional treatment of high‐N (150 kg N ha^–1 y^–1) was established in the broadleaf mixed forest. Soil samples were obtained in July 2008 for analysis. Total organic C (TOC), particulate organic C (POC, > 53 μm), readily oxidizable organic C (ROC), nonreadily oxidizable organic C (NROC), microbial biomass C (MBC), and soil properties were analyzed. Nitrogen addition affected the TOC and its fractions significantly. Labile organic‐C fractions (POC and ROC) in the topsoil (0–10 cm) increased in all the three forests in response to the N‐addition treatments. NROC within the topsoil was higher in the medium‐N and high‐N treatments than in the controls. In the topsoil profiles of the broadleaf forest, N addition decreased MBC and increased TOC, while no significant effect on MBC and TOC occurred in the pine and mixed forests. Overall, elevated N deposition increased the availability of labile organic C (POC and ROC) and the accumulation of NROC within the topsoil irrespective of the forest succession stage, and might enhance the C‐storage capacity of the forest soils.     

南亚热带森林演替过程土壤水分、有机质和交换性阳离子的变化格局

Information on the distribution patterns of soil water content （SWC）, soil organic matter （SOM）, and soil exchangeable cations （SEC） is important for managing forest ecosystems in a sustainable manner. This study investigated how SWC, SOM, and SEC were influenced in forests along a successional gradient, including a regional climax （monsoon evergreen broad-leaved forest, or MEBF）, a transitional forest （coniferous and broad-leaved mixed forest, or MF）, and a pioneer forest （coniferous Masson pine （Pinus rnassoniana） forest, or MPF） of the Dinghushan Biosphere Reserve in the subtropical region of southern China. SWC, SOM, and SEC excluding Ca^2＋ were found to increase in the soil during forest succession, being highest in the top soil layer （0 to 15 cm depth） except for Na^＋. The differences between soil layers were largest in MF. This finding also suggested that the nutrients were enriched in the topsoil when they became increasingly scarce in the soil. There were no significant differences （P = 0.05） among SWC, SOM, and SEC. A linear, positive correlation was found between SWC and SOM. The correlation between SOM and cation exchange capacity （CEC） was statistically significant, which agreed with the theory that the most important factor determining SEC is SOM. The ratio of K^＋ to Na^＋ in the topsoil was about a half of that in the plants of each forest. MF had the lowest exchangeable Ca^2＋ concentration among the three forests and Ca^2＋：K^＋ in MPF was two times higher than that in MF. Understanding the changes of SWC, SOM, and CEC during forest succession would be of great help in protecting all three forests in southern China.     

土地利用方式对湿润亚热带土壤硝化作用的影响

在土壤最大持水量60%和30℃条件下对采自江西的自然土壤(森林和灌丛)和农业利用土壤(稻田、旱地和茶园)进行了实验室培养,研究土地利用对硝化作用的影响。结果表明,由于土壤呈酸性(pH4.2～6.3,平均为4.9),供试土壤的硝化作用很弱甚至缺失。当无外加铵态氮时,土壤的硝化速率与有机氮矿化速率呈显著的线性关系(p<0.01),而与土壤pH无关;当外加铵态氮使基质饱和时,硝化速率与土壤pH显著相关(p<0.01)。农业利用显著提高土壤的硝化作用能力,绝大部分自然土壤(78%)的净硝化速率小于净矿化速率,无机氮以铵态氮为主,而绝大部分农业利用土壤(74%)的净硝化速率大于净矿化速率。农业利用通过提高土壤pH、氮肥施用刺激硝化作用及改善土壤磷素供应状况等途径促进土壤的硝化作用。农业利用土壤硝化作用能力的提高增加了氮肥以硝态氮形态淋失的风险。     

Nitrogen addition change soil N pools with litter removal or not in subtropical forest

ABSTRACT

There are many nitrogen (N) pools in soil, so their availability and different status can give information about bulk soil response to N deposition. However, the different size of N pools in forest soils and the relationship between them have not been well studied under N deposition when considering the role of litter. Here soil in an N-deposition experiment carried out for 5 years in a broad-leaved forest was used as an object to study the response of N pools to N deposition by stepwise extraction using water or solutions containing 0.5 M K₂SO₄, 2.5 M H₂SO₄ (LPI), or 13 M H₂SO₄ (LPII), and calculation of recalcitrant (RC) N pool. Under N control (CT), soil with the presence of litter had a higher N of 23.8–106.8% in the first four pools, but lower of 80.6% in recalcitrant N pool compared with soil with the absence of litter. In the absence of litter, N addition increased soil N in labile pool but decreased N in the RC pool compared to CT and these impacts were greater at high added N (HN) than low-added N (LN) rates. However, in the presence of litter, LN increased the amount of N in the K₂SO₄- extracted pool and HN reduced that in the water extracted pool. Additionally, LN and HN increased TN in the RC pool and HN increased the total soluble N (TSN) in the LPI and LPII pool. N changes in the water extraction pool were attributed to inorganic N, whereas they were NH₄ ⁺ and soluble organic N (SON) in the K₂SO₄-extracted, LPI, and LPII pools. In the presence of litter, HN increased the SON concentration in the K₂SO₄, LPI, and LPII extractions; thus, SON may be a potentially important N form for N availability. These results suggested that N additions improve the accumulation of N in RC pool with the presence of litter. The different effects of N additions on soil N pool or N form in each pool depend on litter present or not.     

Simulated effects of climate change and acid deposition on soil chemical conditions in a Masson Pine forest of SW China

In SW China, acid deposition has been associated with forest damage such as defoliation and mortality due to serious soil acidification. These effects may be exacerbated by ongoing climate change. Understanding the integrated effects of climate change and acid deposition on soil chemistry of forest ecosystems is the key to alleviate forest damage and recover forest health. In particular, the long‐term development of integrated effects is unclear and, thus, prevents setting up cost‐effective controls of atmospheric deposition for improved forest‐health management. We employed the Nutrient Cycling Model (NuCM) to predict the changes of soil chemistry in a Masson pine (Pinus massoniana) forest at Tie‐Shan‐Ping (nearby Chongqing, the biggest city in SW China) under two scenarios of climate change and five scenarios of atmospheric deposition. Field‐monitoring data were used to calibrate and validate the NuCM model. It is shown that the maintaining of current acid deposition both in its quantity and composition would not enhance soil acidification although it would take further 20 years to reach a steady state with higher SO$ _4^{2-} $ and Ca²⁺ concentrations in soil solution. This simulated trend is in contradiction to the decrease of soil pH observed in field during last several decades. The possible reason of this may be the obviously elevated deposition of Ca²⁺, which may help to raise the pH of soil solution but occurred only in recent several years following the rapid increase of local construction industry. However, this enhanced Ca²⁺ input may not be maintained for long time. A decreased S input would lead to some positive effects on soil chemistry such as the increase of soil base saturation. A high N deposition has implicated the forest ecosystem to be saturated with N, and increased N input will further aggravate soil acidification and nutrient imbalances. The future climate change projected by IPCC, i.e., the increase of temperature and rainfall may slightly enhance the negative effect of increased N input. The integrated effect of climate change and N‐deposition increase may counteract the positive effects of decreased S input to a considerable degree. This supports the need for a rigorous implementation of new technology to decrease the emission of both S and N.     

Response of soil respiration to nitrogen addition in two subtropical forest types

Anthropogenic activities have increased nitrogen (N) deposition in terrestrial ecosystems, which directly and indirectly affects soil biogeochemical processes, including soil respiration. However, the effects of the increases in N availability on soil respiration are not fully understood. In this study, soil respiration was measured using an infrared gas analyzer system with soil chambers under four N treatments (0, 5, 15, and 30 g N m^-2 year^-1 as control, low N (LN), moderate N (MN), and high N (HN), respectively) in camphor tree and slash pine forests in subtropical China. Results showed that soil respiration rates decreased by 37% in the camphor tree forest and 27% in the slash pine forest on average on an annual base, respectively, in the N-fertilized treatments when compared with the control. No significant differences were found in the soil respiration rate among the LN, MN, and HN treatments in both forest types as these fertilized plots reached an adequate N content zone. In addition, soil microbial biomass carbon (C) content and fine root biomass declined in N-treated plots compared to the control. Our results indicated that elevated N deposition might alter the tree growth pattern, C partitioning, and microbial activity, which further affect soil C sequestration by reducing soil respiration in subtropical forests of China.