Changes in the phenolic composition of virgin olive oil from young trees (Olea europaea L. cv. Arbequina) grown under linear irrigation strategies.

This study reports the HPLC profiles of phenolic compounds of virgin olive oils obtained from young olive trees (Olea europaea L. cv. Arbequina) and how the application of a linear irrigation strategy affected these. Hydroxytyrosol, tyrosol, vanillic acid, vanillin, 4-(acetoxyethyl)-1,2-dihydroxybenzene, p-coumaric acid, the dialdehydic form of elenolic acid linked to hydroxytyrosol and to tyrosol, lignans, and the oleuropein aglycon were found in all the oils. Hydroxytyrosol, tyrosol, vanillic acid, and p-coumaric acid contents in the oils were unaffected by linear irrigation. The concentration of lignans was lower in the oils from the least irrigated treatment and the concentration of vanillin increased as the amount of irrigation water applied to olive trees increased. However, 4-(acetoxyethyl)-1,2-dihydroxybenzene, the dialdehydic form of elenolic acid linked to hydroxytyrosol and to tyrosol, and the oleuropein aglycon, all of them hydroxyphenyl derivatives, decreased as the level of irrigation water increased. The latter three compounds represented the most considerable part of the phenolic fraction of the oils and they were shown to be correlated to the oxidative stability, the bitter index (K(225)), and the bitter, pungent, and sweet sensory attributes. Linear irrigation strategy changed the profile of the oil phenolic compounds and, therefore, changed both the organoleptic properties and the antioxidant capacity of the product.     

Phenolic and volatile compounds of extra virgin olive oil (Olea europaea L. Cv. Cornicabra) with regard to fruit ripening and irrigation management

This study investigated the effect of both the degree of ripening of the olive fruit and irrigation management-rain-fed, two different regulated deficit irrigations (RDI), the method proposed by the Food and Agriculture Organization of the United Nations (known as FAO), and 125 FAO (125% FAO)-on the phenolic and volatile composition of Cornicabra virgin olive oils obtained during two crop seasons. Secoiridoid phenolic derivatives greatly decreased upon increase of both irrigation and ripening, for example, the 3,4-DHPEA-EDA content decreased from 770 to 450 mg/kg through fruit ripening under rain-fed conditions and from 676 to 388 mg/kg from rain-fed conditions to FAO irrigation treatment (at a ripeness index of approximately 4). Moreover, secoiridoid derivatives of hydroxytyrosol decreased more than those of tyrosol. The levels of major volatile components decreased in the course of ripening but were higher in irrigated olive oils: for example, the E-2-hexenal content ranged between 4.2 and 2.6 mg/kg (expressed as 4-methyl-2-pentanol) over fruit maturation under rain-fed conditions and between 8.0 and 3.5 mg/kg under FAO scheduling. It is important to note that where water was applied only from the beginning of August (RDI-2), when oil begins to accumulate in the fruit, the resulting virgin olive oil presented a phenol and volatile profile similar to those of the FAO and 125 FAO methods, but with a considerable reduction in the amount of water supplied to the olive orchard.     

Effect of regulated deficit irrigation and crop load on the antioxidant compounds of peaches

The use of regulated deficit irrigation (RDI) strategies is becoming a common practice in areas with low water availability. Little information is available about the effects of RDI on the antioxidant content of fruits. In this study, the influence of RDI on the content of vitamin C, phenolic compounds and carotenoids was investigated. Two irrigation strategies, fully irrigated (FI) and RDI, were compared at two levels of thinning, commercial and half of the commercial crop load. RDI strategies affected the content of vitamin C, phenolics and carotenoids of Flordastar peaches. RDI caused fruit peel stress lowering the content of vitamin C and carotenoids, while increasing the phenolic content, mainly anthocyanins and procyanidins. Fruit weight was the only quality index influenced by the crop load as it increased in FI fruits at low crop load. In general, fruits from commercial crop load had slightly higher content of antioxidants to fruits from low crop load, although these influences were only observed in the peel. Additionally, the influence of irrigation controlled by two sensors related to plant water level, maximum daily trunk shrinkage (MDS) and sap flow (SF) on the antioxidant constituents of peaches was evaluated. The response of the fruits to SF sensor was similar to that observed for RDI strategy. According to the tested water sensors, SF did not act as a good plant-based water indicator for use in irrigation scheduling, as it caused an increase in the content of phenolics, similar to that observed for fruits subjected to RDI. Therefore, selection of RDI strategies and plant water indicators should be taken into account as they affect the content of antioxidants of peaches.     

Phenolic compounds in Spanish olive oils.

Phenolic compounds in Spanish virgin olive oils were characterized by HPLC. Simple phenols such as hydroxytyrosol, tyrosol, vanillic acid, p-coumaric acid, ferulic acid, and vanillin were found in most of the oils. The flavonoids apigenin and luteolin were also found in most of the oils. The dialdehydic form of elenolic acid linked to tyrosol and hydroxytyrosol was also detected, as were oleuropein and ligstroside aglycons. The structure of a new compound was elucidated by MS and NMR as being that of 4-(acetoxyethyl)-1,2-dihydroxybenzene. Changes of phenolic compounds in virgin olive oils with maturation of fruits were also studied. Hydroxytyrosol, tyrosol, and luteolin increased their concentration in oils with maturation of fruits. On the contrary, glucoside aglycons diminished their concentration with maturation. No clear tendency was observed for the rest of the phenolic compounds identified.     

调亏灌溉对滴灌成龄香梨果树生长及果实产量的影响

调亏灌溉对果树节水、提高果实产量和品质具有一定效果。2009-2010年,在新疆库尔勒巴州农业科学研究所进行试验,研究滴灌调亏时间及土壤水分亏缺程度对树龄24a的成龄库尔勒香梨果树生长及产量的影响。果实细胞分裂期、果实缓慢膨大期、果实细胞分裂期至缓慢膨大期,分别进行中度土壤水分亏缺与重度土壤水分亏缺灌溉。中度土壤水分亏缺的灌水量为美国A级蒸发皿蒸发量(Ep)的60%,重度土壤水分亏缺的灌水量为Ep的40%。其它时段灌水量与对照相同,为Ep的80%;对照处理整个生育期灌水量均为Ep的80%。灌溉周期为7d。结果表明,前2个生长阶段的调亏灌溉均抑制了香梨树的营养生长,提高了香梨果实产量和灌溉水利用效率。各调亏处理的夏季修剪量比对照减小了8.4%～43.2%。2a内,细胞分裂期重度调亏处理,产量分别比对照增加了15.5%和19.2%,较对照节水9.7%和8.1%;果实缓慢膨大期中度调亏处理,产量分别比对照增加了14.0%和18.0%,较对照节水13.2%和11.3%;果实细胞分裂期及果实缓慢膨大期的重度调亏处理,产量分别比对照减少了15.4%和13.2%,较对照节水34.7%和28.4%。调亏灌溉对香梨果实品质无显著影响。结果对成龄库尔勒香梨灌溉管理具有指导意义。     

Phenolic compounds in olive oils intended for refining: formation of 4-ethylphenol during olive paste storage

The phenolic composition of "lampante olive oil", "crude olive pomace oil", and "second centrifugation olive oil" was characterized by high-performance liquid chromatography with UV, fluorescence, and mass spectrometry detection. The phenolic profile of these olive oils intended for refining was rather similar to that previously reported for virgin olive oil. However, a new compound was found in these oils, which is mainly responsible of their foul odor. It was identified as 4-ethylphenol by comparison of its UV and mass spectra with those of a commercial standard. Although 4-ethylphenol was discovered in all oils intended for refining, its presence was particularly significant in "second centrifugation olive oils", its concentration increasing with time of olive paste storage. Similar trends were observed for hydroxytyrosol, hydroxytyrosol acetate, tyrosol, and catechol, the concentration of these substances reaching values of up to 600 mg/kg of oil, which makes their recovery for food, cosmetic, or pharmaceutical purposes attractive.     

Effect of the maturation process of the olive fruit on the phenolic fraction of drupes and oils from Arbequina, Farga, and Morrut cultivars

The purpose of the work was to investigate the effect of the maturation process of the olive fruit on the phenolic fraction of drupes and oils from Arbequina, Farga, and Morrut cultivars. The level in the phenolic content of olive drupes declines rapidly during the black maturation phase. A general decreasing trend was observed too in the phenolic content of olive oils during the ripening process in the three varieties studied. Important differences in the high-performance liquid chromatography profile between varieties were observed. These included the presence of very low amounts of lignans in olive oils proceeding from the Morrut cultivar, and the presence of three peaks after elution of 3,4-DHPEA-EDA in the Farga and Morrut cultivars, which could be used as differentiating parameters. Sensory profile differences were observed between olive cultivars and due to the ripening process.     

调亏灌溉促进涌泉根灌枣树生长提高产量

调亏灌溉是作物通过主动调节自身营养达到节水和提高果实产量等目的。该文通过微灌枣树试验,研究了涌泉根灌下调亏灌溉对山地枣树生长与产量的影响。2013年分别在萌芽展叶期和开花坐果期进行轻度、中度和重度3个调亏水平调亏处理试验。结果表明:轻度和中度水分亏缺均对枣吊的生长起到抑制作用,能够有效减少新梢生长及夏季修剪量,而对枣树果实的生长起到了促进作用,达到增产的目的。轻度、中度和重度水分亏缺与充分灌溉相比,枣吊长度分别减少了7.2%、13.2%和19.7%(P0.05)。枣树坐果期,果实生长缓慢,轻度、中度、重度调亏以及充分灌溉果实生长量分别为果实最终体积的14.5%、14.1%、13.8%和13.5%。与充分灌溉相比,轻度调亏的枣树最终产量提高了22.1%(P0.05)。可见,调亏灌溉会较为显著的影响枣树的最终产量。适宜的水分亏缺对枣树果实生长与产量有促进作用,且提高了水分利用率。     

调控亏水度灌溉对成龄桃树生长和产量的影响

自1988到1990年,在北京市林业果树研究所桃园17年生的桃树上进行了调控亏水度灌溉法(RDI-Regulated Deficit Irrigation)的试验研究。试验设有3个灌溉水平,即在果实细胞分裂完成后至快速膨大前的一段时间内果实的缓慢生长阶段(RDI期间),分别按80％(对照),40％,20％美国A级皿蒸发量补给。测得的各处理桃树的叶水势情况为:在RDI期间供水量少(处在水应力状态)的桃树,其叶水势较对照低(负的最值大);当RDI结束后各处理同样充分灌水,结果表明,此间曾经受过水应力作用的桃树的叶水势较对照(80％)高(负的量值小)。这种水应力作用的结果表现在对桃树生长量的影响上为:RDI期间,枝条的生长量明显为水势的降低所抑制;而在后期桃果实快速膨大时,经受过RDI的桃树上的果实因水势较高而膨大更快,收获时单果重更大,成熟果实略多,从而提高了产量。另外经受RDI的果树用水量明显减少,从而大幅度提高了水的利用效率。     

Comparative study on phenolic content and antioxidant activity during maturation of the olive cultivar Chemlali from Tunisia

For the first time the identification and quantification of phenolic compounds of the Olea europaea L. cv. Chemlali olive were carried out to examine their profile during maturation. The phenolic composition was studied by using reverse-phase high-performance liquid chromatography during all steps of fruit development. Oleuropein is the abundant phenolic compound in Chemlali olive, and its concentration increases during maturation. An indirect relationship between oleuropein content in olive fruit and hydroxytyrosol was observed. Weak changes in the amounts of the other phenolic monomers and flavonoids were also observed. The total phenolic content varied from 6 to 16 g/kg expressed as pyrogallol equivalents. Its highest level was found at the last maturation period. The antioxidant capacity of olive extracts was evaluated by measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl. The IC(50) values of the olive extract ranged from 3.2 to 1.5 microg/mL. There was a correlation between antioxidant activity and total phenolic content of samples. The antioxidant activity increased with maturation. This could be attributed to the increase of the tolal phenol level with fruit development.     

Hydroxytyrosol 4-beta-D-glucoside,an important phenolic compound in olive fruits and derived products

There is increasing interest in olive polyphenols because of their biological properties as well as their contribution to the color, taste, and shelf life of olive products. However, some of these compounds remain unidentified. It has been shown that hydroxytyrosol 4-beta-D-glucoside (4-beta-D-glucosyl-3-hydroxyphenylethanol) coeluted with hydroxytyrosol [(3,4-dihydroxyphenyl)ethanol] under reversed phase conditions in the phenolic chromatograms of olive pulp, vegetation water, and pomace of olive oil processing. A method to separate this compound from hydroxytyrosol by HPLC has been developed. The concentration of this glucoside increased in olive pulp with maturation and could be the main phenolic compound in mature olives. In contrast, the presence of this compound was not detected in olive oil by using HPLC-MS. The compound must be considered both in table olives and olive oil processing because of its glucose and hydroxytyrosol contribution to these products.     

Evaluation of the influence of thermal oxidation on the phenolic composition and on the antioxidant activity of extra-virgin olive oils

A comparison between the results obtained by using HPLC-UV, HPLC-MS, and CE-UV for characterizing the deterioration of extra-virgin olive oil during heating (180 degrees C) was investigated, taking into account phenolic compounds. The concentration of several compounds belonging to four families of phenols (simple phenols, lignans, complex phenols, and phenolic acids) was determined in the samples after the thermal treatment by all three techniques. Hydroxytyrosol, elenolic acid, decarboxymethyl oleuropein aglycon, and oleuropein aglycon reduced their concentration with the thermal treatment more quickly than other phenolic compounds present in olive oil. HYTY-Ac and Lig Agl were demonstrated to be quite resistant to this kind of treatment, and the behavior of lignans could be outstanding, as they belong to the family most resistant to thermal treatment. Several "unknown" compounds were determined in the phenolic profiles of the oils after the thermal treatment, and their presence was confirmed in refined olive oils. The oxidative stability index (OSI time) was reduced from 25 to 5 h after 3 h of heating, whereas the peroxide value showed a minimum after 1 h of heating.     

Comparison of the concentrations of phenolic compounds in olive oils and other plant oils: correlation with antimicrobial activity

The antimicrobial activity of different edible vegetable oils was studied. In vitro results revealed that the oils from olive fruits had a strong bactericidal action against a broad spectrum of microorganisms, this effect being higher in general against Gram-positive than Gram-negative bacteria. Thus, olive oils showed bactericidal activity not only against harmful bacteria of the intestinal microbiota (Clostridium perfringens and Escherichia coli) also against beneficial microorganisms such as Lactobacillus acidophilus and Bifidobacterium bifidum. Otherwise, most of the foodborne pathogens tested (Listeria monocytogenes, Staphylococcus aureus, Salmonella enterica, Yersinia sp., and Shigella sonnei) did not survive after 1 h of contact with olive oils. The dialdehydic form of decarboxymethyl oleuropein and ligstroside aglycons, hydroxytyrosol and tyrosol, were the phenolic compounds that statistically correlated with bacterial survival. These findings were confirmed by testing each individual phenolic compound, isolated by HPLC, against L. monocytogenes. In particular, the dialdehydic form of decarboxymethyl ligstroside aglycon showed a potent antimicrobial activity. These results indicate that not all oils classified as "olive oil" had similar bactericidal effects and that this bioactivity depended on their content of certain phenolic compounds.     

Effects of natural phenolic compounds on the antioxidant activity of lactoferrin in liposomes and oil-in-water emulsions

The effect of natural phenolic compounds on the antioxidant and prooxidant activity of lactoferrin was studied in liposomes and oil-in-water emulsions containing iron. The antioxidants tested with lactoferrin were alpha-tocopherol, ferulic acid, coumaric acid, tyrosol, and natural phenolic extracts obtained from three different extra-virgin olive oils and olive mill wastewater. The natural extracts of olive oils and mill wastewaters were composed mainly of polyphenols and simple phenolics, respectively. Lipid oxidation at 30 degrees C was determined by the formation of hydroperoxides and fluorescent compounds resulting from oxidized lipid interactions. All phenolic compounds showed synergistic properties in reinforcing the antioxidant activity of lactoferrin in lipid systems containing iron. The highest synergistic effects were observed for the phenolic extracts rich in polyphenols of extra-virgin olive oils and lactoferrin. This synergistic effect was higher in liposomes than in emulsions.     

调亏灌溉对冬小麦生理机制及水分利用效率的影响

在人工控制试验条件下，采用子母盆栽土培法，以冬小麦为试验材料进行了调亏灌溉试验研究。结果表明，适时适度的水分调亏显著抑制蒸腾速率，而光合速率下降不明显，复水后光合速率又具有超补偿效应，光合产物具有超补偿积累，且有利于向籽粒运转与分配；抑制营养生长，促进生殖生长。冬小麦调亏灌溉的适宜时段为三叶—返青，调亏度为40%～60%田间持水率(θ_F)，历时约55 d；平均比对照增产0.88%～8.25%，节水12.80～18.55%，水分利用效率提高15.96%～32.98%。     

Development of a phenol-enriched olive oil with both its own phenolic compounds and complementary phenols from thyme

Besides affecting the oil's sensorial characteristics, the presence of herbs and spices has an impact on the nutritional value of the flavored oils. The aim of the study was to develop a new product based on the phenol-enrichment of a virgin olive oil with both its own phenolic compounds (secoiridoid derivatives) plus additional complementary phenols from thyme (flavonoids). We studied the effect of the addition of phenolic extracts (olive cake and thyme) on phenolic composition, oxidative stability, antioxidant activity, and bitter sensory attribute of olive oils. Results showed that flavonoids from thyme appeared to have higher transference ratios (average 89.7%) from the phenolic extract to oil, whereas secoiridoids from olive presented lower transference ratios (average 35.3%). The bitter sensory attribute of the phenol-enriched oils diminished with an increase of the concentration of phenols from thyme, which might denote an improvement in the consumer acceptance.     

Effect of the technological and agronomical factors on pigment transfer during olive oil extraction

The aim of this work was to determine the transfer of the chloroplast pigment fractions during the virgin olive oil extraction process, in relation to different factors: the ripening stage of the olive fruits, the irrigation water applied to the olive tree, and the addition of natural microtalc (NMT) during the oil extraction process. Results showed that the percentage of chloroplast pigments transferred from the olive paste to the oil increases with the ripening of the olive fruit (raw material). An excess of the water irrigation applied to the olive tree shows a reduction in the biosynthesis of chloroplast pigments in olive fruits, which is reflected in a low concentration in the virgin oils. Furthermore, the percentage of pigment transfer from the olive paste to the oil during the extraction process is reduced by irrigation, mainly of the chlorophyll fraction. The addition of NMT during the malaxation step produced an increase in the percentage of the total pigments transferred from the olive paste to the oil, in relation to nonaddition.     

河西绿洲菘蓝生长、光合特性及品质对膜下滴灌调亏的响应

通过菘蓝的水分控制试验(2016年),研究了不同生育期不同梯度调亏灌溉对河西绿洲菘蓝生长、光合特性及产量品质的影响。试验以河西走廊中部张掖市水务局国家重点灌溉试验站为平台,甘肃农业大学中草药系自繁菘蓝种子为供试材料,采用不同生育期和不同梯度调亏灌溉方式二因素随机区组试验设计,共9个处理。在每个生育期测定菘蓝光合参数,收获后测定新鲜板蓝根产量及品质,为河西绿洲灌区合理种植和提高菘蓝产量品质及可持续发展提供理论依据。结果表明:(1)营养生长和肉质根生长期水分亏缺显著降低了菘蓝叶片净光合速率、蒸腾速率和气孔导度,且降幅随水分亏缺程度加大而增大。(2)水分亏缺造成菘蓝产量降低,调亏处理中肉质根成熟期轻度水分亏缺产量最高(7 342.05kg/hm~2),较充分灌溉降低5.32%(P0.05)。(3)苗期轻度水分亏缺水分利用效率最高(2.14kg/m~3),肉质根成熟期轻度水分亏缺次之。(4)肉质根成熟期轻度调亏处理的菘蓝综合品质最优,其菘蓝靛蓝、靛玉红、(R,S)-告依春含量比生育期充分灌溉提高了0.09,0.41,0.007 8mg/g,但多糖含量下降3.37mg/g。因此,综合考虑菘蓝产量、水分利用效率及品质等指标,肉质根成熟期进行轻度调亏,即该时期土壤相对含水率为60%~90%,可以作为河西走廊绿洲灌区菘蓝最优栽培灌溉的一种方式。     

The influence of bearing cycles on olive oil quality response to irrigation

Five rates of water application were applied in a 4 year study on olive (Olea europaea) varieties 'Barnea' and 'Souri'. Increased irrigation lead to increased tree-scale oil yields, lower polyphenol content, and, frequently, higher oil acidity. These effects were predominant in "off" years. The fatty acid profile was influenced primarily by bearing level and variety and secondarily by irrigation rate. The saturated to unsaturated fatty acid ratio was higher in "off" than in "on" years, and the monounsaturated fatty acid to polyunsaturated fatty acid ratio was higher in "on" years as a result of the fact that oleic and stearic acids were higher in "on" years, while palmitic, palmitoleic, and linoleic acids were greater in "off" years. Squalene was higher in 'Souri' than in 'Barnea' oils, was not affected by bearing cycle, and was consistently lower in oil from trees receiving the lowest irrigation level.     

Analysis of total contents of hydroxytyrosol and tyrosol in olive oils

The most abundant phenolic compounds in olive oils are the phenethyl alcohols hydroxytyrosol and tyrosol. An optimized method to quantify the total concentration of these substances in olive oils has been described. It consists of the acid hydrolysis of the aglycons and the extraction of phenethyl alcohols with a 2 M HCl solution. Recovery of the phenethyl alcohols from oils was very high (<1% remained in the extracted oils), and the limits of quantification (LOQ) were 0.8 and 1.4 mg/kg for hydroxytyrosol and tyrosol, respectively. Precision values, both intraday and interday, remained below 3% for both compounds. The final optimized method allowed for the analysis of several types of commercial olive oils to evaluate their hydroxytyrosol and tyrosol contents. The results show that this method is simple, robust, and reliable for a routine analysis of the total concentration of these substances in olive oils.