The value of timothy (Phleum pratense L.) in ryegrass — timothy mixtures managed to simulate intensive grazing

The performance of timothy in mixtures with perennial ryegrass was assessed under a simulated intensive grazing management over two harvest years in 1974–75. Three seed rates of S23 perennial ryegrass were factorially combined with three rates of Scots timothy and compared with pure stands of each grass. All were sown with Huia white clover. When cut six times at monthly intervals and with an annual N input of 350 kg ha^?1, there were no significant differences in total DM production in either year. The 2-year mean DM yield for the nine mixtures and six pure swards was 9·77 t ha^?1 (range 9·34–10·16). Compared with the pure ryegrass swards, in both years the ryegrass-timothy mixtures produced earlier spring growth but were significantly lower yielding at the second cut. Over the first five cuts the proptortion of timothy in the three mixtures with 22·4 kg ha^?1 ryegrass seed averaged 26% in the first year and 37% in the second. Corresponding calculated mean DM yields of timothy were 2·75 and 3·00 t ha^?1. It is concluded that an early timothy variety is capable of competing with a late-heading perennial ryegrass in frequently cut swards managed to simulate intensive grazing. The strong development of timothy in the dry summer of 1975 suggests that in mixtures of late perennial ryegrass varieties, an early variety of timothy should be beneficial for its spring growth in grazed swards.     

‘MaxClover’ grazing experiment: I. Annual yields,botanical composition and growth rates of six dryland pastures over nine years

Six dryland pastures were established at Lincoln University, Canterbury, New Zealand, in February 2002. Production and persistence of cocksfoot pastures established with subterranean, balansa, white or Caucasian clovers, and a perennial ryegrass‐white clover control and a lucerne monoculture were monitored for nine years. Total annual dry‐matter (10.0–18·5 t DM ha^?1) and sown legume yields from the lucerne monoculture exceeded those from the grass‐based pastures in all but one year. The lowest lucerne yield (10 t ha^?1 yr^?1) occurred in Year 4, when spring snow caused ungrazed lucerne to lodge and senesce. Cocksfoot with subterranean clover was the most productive grass‐based pasture. Yields were 8·7–13·0 t DM ha^?1 annually. Subterranean clover yields were 2·4–3·7 t ha^?1 in six of the nine years which represented 26–32% of total annual production. In all cocksfoot‐based pastures, the contribution of sown pasture components decreased at a rate equivalent to 3·3 ± 0·05% per year (R² = 0·83) and sown components accounted for 65% of total yield in Year 9. In contrast, sown components represented only 13% of total yield in the ryegrass‐white clover pastures in Year 9, and their contribution declined at 10·1 ± 0·9% per year (R² = 0·94). By Year 9, 79% of the 6.6 t ha^?1 produced from the ryegrass‐white clover pasture was from unsown species and 7% was dead material. For maximum production and persistence, dryland farmers on 450–780 mm yr^?1 rainfall should grow lucerne or cocksfoot‐subterranean clover pastures in preference to ryegrass and white clover. Inclusion of white clover as a secondary legume component to sub clover would offer opportunities to respond to unpredictable summer rainfall after sub clover has set seed.     

Micronutrient concentrations in common and novel forage species and varieties grown on two contrasting soils

We investigated differences between forage species with regard to micronutrients that are essential to sustain livestock health. Five grasses (timothy, perennial ryegrass, meadow fescue, tall fescue and cocksfoot), three legumes (red clover, white clover and birdsfoot trefoil) and four forbs (ribwort plantain, salad burnet, caraway and chicory) were grown on one micronutrient‐poor/low pH soil and one micronutrient‐rich/high pH soil (outdoor pot experiment). In addition, six grasses (timothy, perennial ryegrass, meadow fescue, tall fescue, Festulolium hybrid and cocksfoot) and one legume (red clover) were field‐grown on the micronutrient‐poor soil. Of the twelve pot‐grown species, herbage of chicory, red clover and white clover generally had the highest micronutrient concentrations (maximum Co, Cu, Fe and Zn concentrations were 0·23, 9·8, 233 and 109 mg kg^?1 DM, respectively), except for Mo, which was highest in the clovers (10·6 mg kg^?1 DM), and Mn, which was highest in cocksfoot (375 mg kg^?1 DM). Soil type had the strongest effect on plant Mo and Mn concentrations. We also investigated differences in micronutrients between varieties, but they were generally few and negligible. The results indicate that choice of forage species is of major importance for micronutrient concentrations in herbage and that soil type exerts a major effect through pH. Forage of chicory, red clover and white clover generally met the requirements of high‐yielding dairy cows with regard to most micronutrients; therefore, diversification of seed mixtures so as to include these species could increase micronutrient concentrations in forage.     

A comparison of diploid and tetraploid perennial ryegrass and tetraploid ryegrass/white clover swards under continuous sheep stocking at controlled sward heights. 3. Sward characteristics and animal output, years 4–8

Results for years 4–8 of a long-term grazing experiment on swards of a diploid perennial ryegrass (Lolium perenne), var. Contender (D swards), a tetraploid ryegrass, var. Condesa (T swards) and Condesa with S184 white clover (Trifolium repens) (TC swards), direct sown in May 1987, are presented. The swards were continuously stocked with sheep from 1988 to 1990, as previously reported, and for a further 5 years, 1991–95, at a target sward surface height (SSH) of 4–6 cm. Control of sward height was successfully achieved by variable stocking, except in 1993 when paddocks were set stocked and the resulting mean SSH was 9·3 cm. Grass swards received on average 160 kg N ha^?1 year^?1; grass/clover swards were mainly not fertilized with N with the exception that they were given 30 kg N ha^?1 as a remedial mid-summer application during a period of low herbage mass on offer in 1994 and 1995. Mean white clover content of the swards fell from 18·2% of herbage dry-matter (DM) in 1992 to 8·5% in 1993, whereas stolon lengths fell from 120 to 58 m m^?2. A return to lower sward heights in 1994–95 resulted in an increase in white clover content to 12·8% by the final sampling in August 1995. Perennial ryegrass content of the grass swards remained high throughout (mean 96·7% in 1995). Perennial ryegrass tiller densities recorded in August 1991, 1993 and 1994 showed consistently significant (P < 0·001) sward differences (3-year mean 16 600, 13 700 and 10 100 perennial ryegrass tillers m^?2 for the D, T and TC swards). In 1994, the year after lax grazing, a low perennial ryegrass tiller density (9100 m^?2) and low white clover content (mean 4·3%) in the TC swards resulted in a much lower herbage bulk density than in the grass swards (April–July means 72, 94 and 44 kg OM ha^?1 cm^?1 for the D, T and TC swards). There was a consistent 40 g d^?1 increase in lamb liveweight gain on the TC swards over the T swards, except in 1994. In that year there was a reduction in lamb liveweight gain of 33 g d^?1 on the TC swards and a significant increase in ewe liveweight loss (117 g d^?1) associated with low herbage bulk density despite optimal sward height. Lamb output (kg liveweight ha^?1) on TC swards reflected white clover content, falling from a similar output to that produced from grass given 160 kg N ha^?1, at 18% white clover DM content, down to 60% of grass + N swards with around 5% clover. A 6% greater output from the T than the D swards was achieved mainly through higher stocking rate. The experiment demonstrated a rapid, loss in white clover under lax grazing, and showed that the relationship between performance and sward height is also dependent on herbage density. High lamb output from a grass/clover sward was only achieved when the clover content was maintained at 15–20% of the herbage DM.     

Liveweight gains of lambs from Caucasian clover/ryegrass and white clover/ryegrass swards on soils of high and low fertility

The high nutritive value and persistence under a wide range of climatic and soil fertility conditions make Caucasian clover a potentially useful forage legume but there is little information about the performance of livestock grazing Caucasian clover/grass swards. This study compared liveweight gains of lambs grazing Caucasian clover/perennial ryegrass and white clover/perennial ryegrass swards on high fertility (Olsen P 20 mg L^?1, SO₄‐S 12 mg kg^?1) and low fertility (Olsen P 11 mg L^?1, SO₄‐S 7 mg kg^?1) soils from 1998 to 2001 in the South Island of New Zealand. Mean annual liveweight gains were 1178 kg ha^?1 for Caucasian clover/perennial ryegrass and 1069 kg ha^?1 for white clover/perennial ryegrass swards at high fertility compared with 1094 kg ha^?1 and 1015 kg ha^?1, respectively, at low fertility. There was a higher mean proportion of clover in Caucasian clover/perennial ryegrass (0·19) than white clover/perennial ryegrass (0·11) swards, but there were no differences in total herbage production between the two clover/perennial ryegrass swards. The mean concentration of crude protein in the herbage of Caucasian clover (302 g kg DM^?1) was higher than that in white clover (287 g kg DM^?1) and grass herbage (227 g kg DM^?1). Estimated mean metabolizable energy concentrations in the herbage were 12·5 MJ kg DM^?1 for the two clovers and 11·6 MJ kg DM^?1 for grass herbage. The difference in liveweight gain between swards on soils of high and low fertility was associated with an increase in total herbage production of similar composition and nutritive value, giving a greater number of grazing days for the swards on soils of high than low fertility.     

Production performance and meat quality of grazing lambs finished on red clover, lucerne or perennial ryegrass swards

The combined benefits of a high crude protein concentration, and possible protein protection and growth‐promoting properties, make forage legumes potentially attractive as a natural means of increasing liveweight gain and time to slaughter of lambs in lamb finishing systems. An experiment was conducted to compare the production performance and meat quality of grazing lambs finished on red clover (Trifolium pratense), lucerne (Medicago sativa) or perennial ryegrass (Lolium perenne) swards. Replicate (n = 2) swards of red clover, lucerne and perennial ryegrass were rotationally grazed by ten ram lambs and ten ewe lambs from weaning until selection for slaughter at UK fat class 3L. Lambs grazing the red clover sward had a significantly higher liveweight gain and required significantly fewer days to slaughter than lambs grazing the lucerne sward (305 g d^?1 vs. 243 g d^?1; 38 d vs. 50 d), which in turn had a higher liveweight gain and required fewer days to slaughter than lambs grazing the perennial ryegrass sward (184 g d^?1; 66 d). Lambs grazing the red clover and lucerne swards had significantly higher herbage intakes than those grazing the perennial ryegrass sward (2·06, 1·72 and 1·16 kg DM d^?1 respectively), but in vivo digestibility of herbage was similar. Lambs grazing the red clover and lucerne swards also had significantly higher serum urea concentrations than those grazing ryegrass (12·5, 11·1 and 6·2 mmol L^?1 respectively). Killing‐out percentage was significantly higher for lambs grazing the red clover sward than for lambs grazing the perennial ryegrass sward (48% vs. 46%). There were no significant effects of finishing system on meat flavour, but meat from lambs finished on the lucerne sward was oxidatively less stable than that from lambs finished on the perennial ryegrass sward. Grazing the forage legume swards significantly increased the proportion of linoleic and linolenic acid in muscle tissue, and therefore the proportion of unsaturated to saturated fatty acids (0·19, 0·16 and 0·12 for the red clover, lucerne and perennial ryegrass swards respectively). However, the n?6/n?3 ratio was significantly lower for the muscle of lambs grazing the perennial ryegrass sward compared with those grazing the forage legume swards (1·13, 1·08 and 0·98 for the red clover, lucerne and perennial ryegrass swards respectively). The results indicate that by grazing lambs on forage legume swards it is possible to increase individual lamb performance without compromising meat quality.     

Red clover for silage: management impacts on herbage yield,nutritive value,ensilability and persistence,and relativity to perennial ryegrass

This 6‐year experiment quantified the impacts of management factors on red clover yield, persistence, nutritive value and ensilability, and compared these with perennial ryegrass receiving inorganic N fertilizer. Within a randomized complete block design, field plots were used to evaluate a 2 (cultivar, Merviot and Ruttinova) × 2 (alone and with perennial ryegrass) × 2 (0 and 50 kg fertilizer N ha^?1 in mid‐March) × 2 (harvest schedule) combination of the factors relating to red clover, and a 2 (harvest schedule) × 4 (0, 50, 100 and 150 kg N ha^?1 for each cut) combination of the factors relating to perennial ryegrass. The early and late harvest schedules both involved four cuts per year, but commenced a fortnight apart. Red clover treatments averaged 14 906 kg dry matter (DM) ha^?1 per year, whereas perennial ryegrass receiving 600 kg inorganic N fertilizer per year averaged 14 803 kg DM ha^?1 per year. There was no yield decline evident across years despite a decline in the proportion of red clover. The early harvest schedule and sowing ryegrass with red clover increased the herbage yield and digestibility. March application of fertilizer N to red clover treatments reduced the annual yield. Early harvest schedule increased and both fertilizer N and sowing with ryegrass decreased the proportion of red clover. Sowing with ryegrass improved the indices of ensilability, but reduced the crude protein content. Both red clover cultivars had similar performance characteristics. A selected red clover‐based treatment, considered to exhibit superior overall production characteristics, outyielded N‐fertilized perennial ryegrass in mid‐season. However, it had poorer digestibility and ensilability indices.     

A comparison of the herbage productivity of Bromus wildenowii cv. Grasslands Matua with four cultivars of Lolium perenne when grown in association with Trifolium repens

In a small-plot trial five grass varieties bromegrass cv. Grasslands Matua, perennial ryegrass CVS. Melle (diploid) and Bastion, Condesa and Meltra (tetraploid) were established as grass/white clover swards with white clover cv. Menna. Productivity was measured under 6-weekIy cutting both without N fertilizer (N_o) and with 100 kg N ha^?1 applied in spring (N₁₀₀) Evaluation was made over 2 harvest years, 1986–87. Total mean annual production of herbage dry matter (DM) in the first harvest year at No and N_o was 5·07 t ha^?1 and 6·93 t ha^?1 respectively. In year 2, corresponding values were 11·81 and 12·67 t ha^?1. In year 1, Matua swards at N_o and N₁₀₀ yielded 5·08 and 6·65 t DM ha^?1 compared with 507 and 70 t DM ha^?1 for the mean of the four ryegrass varieties. In year 2, corresponding values were 12·90 and 12·29 for Matua and 11·54 and 12·78 for the four ryegrasses. In year 1, the digestable organic matter in the dry matter (DOMD) of the Matua swards was lower than that of Melle, Bastion and Condesa at N_O, particularly at the first cut. In year 2, differences in DOMD between treatments and varieties were not significant. The proportion of white clover was found to be higher in the N_o than the N₁₀₀ treatment, and also higher in year 2 in most treatments. For the _No treatment Matua swards had the highest proportion of white clover in year 1 (32% compared with 24% for the mean of the ryegrass varieties) but the lowest proportion in year 2 (27% compared with 60% for the ryegrasses). For the N_o treatment in year 1 clover production was also 43% higher, on average, from the tetraploid treatments than with Melle as the companion grass; for this comparison in year 2 the differences were not significant. It is concluded that Matua bromegrass/white clover swards receiving no N fertilizer may have a good potential under cutting management. However, the evidence from this trial is that in the second year the proportion of white clover is lower with Matua swards than with perennial ryegrass as the companion grass.     

Effects of white clover cultivar and companion grass on winter survival of seedlings in autumn-sown swards

The aim was to study the effects of white clover cultivar and combinations with perennial ryegrass cultivars on seedling establishment in autumn‐sown swards and on winter survival of seedlings. Large‐leaved white clover cv. Alice and small‐leaved white clover cv. Gwenda, and an erect and a prostrate perennial ryegrass cultivar were sown in autumn in pure stands and as four binary grass‐clover mixtures. Mixtures of white clover cv. Huia and Aberherald with perennial ryegrass were also sown. Companion grasses had no significant impact on the establishment of white clover. The number of seedlings of white clover cv. Alice in mixtures (335 m^?2) was higher than cv. Gwenda (183 m^?2) and pure swards had similar white clover population densities as mixed swards. White clover cv. Huia tended to have more seedlings than Aberherald (355 and 205 m^?2 respectively). No stolons were produced prior to a severe winter, because of the late sowing date. Winter survival of clover seedlings was 0·56 in mixtures and 0·69 in pure stands, irrespective of white clover or companion grass cultivar. Stolon development of white clover in autumn is often considered essential for overwintering survival and spring growth. In this study, there was considerable survival of the non‐stoloniferous tap‐rooted seedlings of all four clover cultivars despite a severe winter.     

Impact of spatial heterogeneity of plant species on herbage productivity,herbage quality and ewe and lamb performance of continuously stocked,perennial ryegrass–white clover swards

The benefits of white clover (Trifolium repens L.) in pastures are widely recognized. However, white clover is perceived as being unreliable due to its typically low content and spatial and temporal variability in mixed (grass‐legume) pastures. One solution to increase the clover proportion and quality of herbage available to grazing animals may be to spatially separate clover from grass within the same field. In a field experiment, perennial ryegrass (Lolium perenne L.) and white clover were sown as a mixture and compared with alternating strips of ryegrass and clover (at 1·5 and 3 m widths), or in adjacent monocultures (strips of 18 m width within a 36‐m‐wide field). Pastures were stocked by ewes and lambs for three 10‐month grazing periods. Over the 3 years of the experiment, spatial separation of grass and clover, compared with a grass–clover mixture, increased clover herbage production, although its proportion in the sward declined through time (0·49–0·54 vs 0·34 in the mixture in the first year, 0·28–0·33 vs 0·15 in the second year and 0·03–0·18 vs 0·01 in the third year). Total herbage production in the growing season in the spatially separated treatments decreased from 11384 kg DM ha^?1 in the first year to 8150 kg DM ha^?1 in the third year. Crude protein concentration of clover and grass components in the 18‐m adjacent monoculture treatment was greater than the mixture treatment for both clover (310 vs 280 g kg^?1 DM) and grass (200 vs 180 g kg^?1 DM). There was no clear benefit in liveweight gain beyond the first year in response to spatially separating grass and clover into monocultures within the same field.     

Optimizing legume content and forage yield of mown white clover–Italian ryegrass mixtures through nitrogen fertilization and grass row spacing

Mixed swards of white clover–grass mixtures in highly productive environments often fail to reach the minimum recommended annual clover proportion of about 0·30. This study assessed the effect on clover content and total dry matter (DM) yield of two spring N applications (0 and 45 kg N ha^?1) and two distances between drilled grass‐rows (0·18 and 0·36 m) over 3 years for mown swards of white clover–Italian ryegrass (Trifolium repens–Lolium multiflorum) in binary mixtures in northern Italy. An additional aim was to determine the advantage of association of grass–clover compared with grass and clover monocultures. On average, N fertilization of mixtures resulted in almost 9% higher total yield (P < 0·01; mean response = 18·1 kg of total DM per kg of N) but decreased the clover proportion (0·250 vs. 0·312). Wider grass‐row spacing increased clover proportion (0·327 vs. 0·234; P < 0·01) with no reduction of total DM yield. N fertilization × grass‐row spacing interaction occurred only for clover content (P < 0·01). Without N fertilization, mixtures out‐yielded clover and grass pure stands. With N fertilization, at double rate to pure grass, yields from mixtures were greater than from clover and comparable to Italian ryegrass.     

Selection by sheep and goats for perennial ryegrass and white clover offered over a range of sward height contrasts

The selection by sheep (six Coopworth ewe hoggets, 44·3 ± 4·6 kg live weight) and goats (six Saanen/Anglo‐Nubian yearling males, 38·1 ± 3·8 kg live weight) for perennial ryegrass (Lolium perenne) and white clover (Trifolium repens) and for sward height was measured in two experiments involving paired turves. Pairs of turves with herbage of differing height and of either the same or different plant species were offered. One sward (fixed height species, FHS) was always offered at 130 mm and the other (variable height species, VHS) at 130, 90 or 50 mm. Turves (450 mm × 220 mm) were cut to a soil depth of 100–150 mm from areas of perennial ryegrass and white clover regrown to the desired height after previously being cut to 30 mm. Each turf in a pair was weighed (±1 g) before and after grazing by penned animals maintained on a barley‐based pelleted diet. The number of prehending bites taken from each turf was recorded over a grazing period (128 ± 12 s). Bite mass, bite rate and intake rate were calculated. As the sward height of the VHS turf declined, an increasing proportion of the diet was selected from the 130 mm turf. When averaged over all height contrasts, both animal species selected a higher proportion (0·776 ± 0·026) of their diet from 130‐mm white clover than from 130‐mm perennial ryegrass (0·591 ± 0·018) turves. On average, goats selected a higher proportion (0·721 ± 0·022) of their dry‐matter (DM) intake from the 130‐mm turf than sheep (0·646 ± 0·019), but the effect was not consistent. In contrasts with perennial ryegrass as the VHS (and both perennial ryegrass and white clover as FHS), the proportion of the diet selected from the 130‐mm turf was very similar for both animal species. However, with white clover as the VHS (and both perennial ryegrass and white clover as FHS), goats selected a higher proportion of their intake from the 130‐mm turf to the extent that in the 130‐mm perennial ryegrass/50‐mm white clover contrast sheep showed as strong selection for 50‐mm white clover as goats did for 130‐mm perennial ryegrass. This lesser selection of goats for white clover as its height in a sward declines is likely to contribute to the higher white clover content observed in swards grazed by goats. Bite mass was greater on white clover (246 ± 5 mg DM bite^–1) than on perennial ryegrass (173 ± 5 mg DM bite^–1) and was greater for goats (255 ± 6 mg DM bite^–1) than for sheep (195 ± 5 mg DM bite^–1). Bite rate was greater on perennial ryegrass (45·9 ± 1·0 bites min^–1) than on white clover (39·9 ± 1·0 bites min^–1) and was greater for sheep (45·5 ± 1·1 bites min^–1) than for goats (42·5 ± 1·1 bites min^–1). Apparent intake rate by both sheep and goats was lower (mean, 5·0 ± 0·29 g DM min^–1) on 130 mm perennial ryegrass/white clover than on 130 mm perennial ryegrass/perennial ryegrass (7·0 ± 0·27 g DM min^–1), but was higher (9·62 ± 0·29 g DM min^–1) on 130‐mm white clover/perennial ryegrass than on 130‐mm white clover/white clover (8·2 ± 0·29 g DM min^–1) combinations.     

Effect of defoliation management, based on leaf stage, on perennial ryegrass (Lolium perenne L.), prairie grass (Bromus willdenowii Kunth.) and cocksfoot (Dactylis glomerata L.) under dryland conditions. 2. Nutritive value

A field experiment was undertaken between April 2003 and May 2004 in southern Tasmania, Australia, to quantify and compare changes in the nutritive value of perennial ryegrass (Lolium perenne L.), prairie grass (Bromus willdenowii Kunth.) and cocksfoot (Dactylis glomerata L.) under a defoliation regime based on stage of leaf regrowth. Defoliation interval was based on the time taken for two, three or four leaves per tiller to fully expand. At every defoliation event, samples were collected and analysed for acid‐detergent fibre (ADF), neutral‐detergent fibre (NDF) and total nitrogen (N) concentrations and to estimate metabolizable energy (ME) and digestible dry matter (DDM) concentrations. Amounts of crude protein (CP) and metabolizable energy (MJ) per hectare values were subsequently calculated. There was a significantly lower (P < 0·001) NDF concentration for perennial ryegrass compared with prairie grass and cocksfoot, and a significantly lower (P < 0·001) ADF concentration for cocksfoot compared with prairie grass and perennial ryegrass, regardless of defoliation interval. The CP concentration of cocksfoot was significantly greater (P < 0·001) compared with the CP concentrations of prairie grass and perennial ryegrass. The estimated ME concentrations in cocksfoot were high enough to satisfy the requirements of a lactating dairy cow, with defoliation at or before the four‐leaf stage maintaining ME concentrations between 10·7 and 10·9 MJ kg^?1 DM, and minimizing reproductive plant development. The ME concentrations of prairie grass (10·2–10·4 MJ kg^?1 DM) were significantly lower (P < 0·001) than for cocksfoot (as above) and perennial ryegrass (11·4–11·6 MJ kg^?1 DM) but a higher DM production per hectare resulted in prairie grass providing the greatest amounts of ME ha^?1.     

Effects of cutting frequency on plant production, N-uptake and N2 fixation in above- and below-ground plant biomass of perennial ryegrass–white clover swards

Nitrogen (N), accumulating in stubble, stolons and roots, is an important component in N balances in perennial ryegrass–white clover swards, and the effects of cutting frequency on the biomass of above‐ and below‐harvest height were studied during two consecutive years. Total dry matter (DM) and total N production, and N₂ fixation, were measured at two cutting frequencies imposed in the summers of two years either by cutting infrequently at monthly intervals to simulate mowing or by frequent cutting at weekly intervals to simulate grazing. Total DM production harvested was in the range of 3000–7000 kg DM ha^?1 with lower DM production associated with the frequent cutting treatment, and it was significantly affected by the different weather conditions in the two years. The higher cutting frequency also reduced the biomass below harvest height but the different weather conditions between years had less effect on stubble and, in particular, biomass of roots. The biomass of roots of white clover was significantly lower than that of roots of perennial ryegrass and remained at a relatively constant level (200–500 kg DM ha^?1) throughout the experiment, whereas the biomass of perennial ryegrass roots increased from 2400 kg DM ha^?1 in the year of establishment to 10 200 kg DM ha^?1 in the infrequent cutting treatment and 6650 kg DM ha^?1 in the frequent cutting treatment by the end of the experiment, giving shoot:root ratios of 4·7–16·6 and 0·5–1·6 for white clover and perennial ryegrass respectively. Annual N₂ fixation was in the range of 28–214 kg N ha^?1, and the proportion of N fixed in stolons and roots was on average 0·28. However, as weather conditions affect the harvested DM production and the shoot:root ratio, care must be taken when estimating total N₂ fixation based on an assumed or fixed shoot:root ratio.     

Characteristics of baled silage made from first and second harvests of wilted and severely wilted forages

First and second harvests of lucerne (Medicago sativa L.), perennial ryegrass (Lolium perenne L.) and a lucerne–perennial ryegrass mixture [80 or 144 g kg^?1 dry matter (DM) of ryegrass] at the first and second harvests were cut and conditioned, wilted to 500 or 700 g DM kg^?1 then baled and stretch‐wrapped for silage on the same dates. Lucerne bales were denser (411 kg m^?3) than bales of perennial ryegrass (331 kg m^?3) (P < 0·05). After an 8‐month storage period, silage made from high DM‐content forage had a higher concentration of neutral‐detergent fibre (NDF) and was less digestible than that made from low DM‐content forage. Daily DM intakes by beef steers, when the silages of the second harvest were fed ad libitum, were 31·2, 31·2 and 22·3 g kg^?1 live weight for lucerne, lucerne–perennial ryegrass mixture and perennial ryegrass silages, respectively (P < 0·01), when the herbage had been wilted to 500 g kg^?1. In vivo digestibility of NDF in the lucerne–perennial ryegrass mixture silage (0·587) was significantly lower than that of perennial ryegrass silage (0·763) but higher than lucerne silage (0·518). Higher intakes of baled lucerne silage tended to offset its lower digestibility values. Lucerne–perennial ryegrass mixture silage had a higher DM and NDF digestibility than lucerne silage, indicating perhaps the presence of associative effects.     

White clover selected for enhanced competitive ability widens the compatibility with grasses and favours the optimization of legume content and forage yield in mown clover‐grass mixtures

White clover‐grass mixtures should provide swards with a high dry matter (DM) yield with a clover proportion of at least 0·30. This study assessed the compatibility of one white clover variety selected for competitive ability (Giga) and four other large‐leaved clover varieties (Aran, Espanso, Fantastico and Regal) in mown binary mixtures with each of four grass companions (cocksfoot cv. Padania, hybrid ryegrass cv. AberExcel, Italian ryegrass cv. Crema, and tall fescue cv. Magno) over 3 years in northern Italy. On average, the mixtures including Giga had at least a two‐fold greater clover proportion in the total harvested DM (P < 0·01) and contained fewer weeds (P < 0·10) than those with other clover varieties, and also tended to have the highest total DM yields. On average, mixtures containing AberExcel had clover proportions of >0·25 but gave the lowest total DM yields, whereas those of Magno maximized total DM and had a higher proportion of clover than Padania or Crema. No clover × grass interaction was detected (P > 0·05). The association with Giga allowed the tall fescue‐based mixture to attain a clover proportion 0·331 along with a top‐yielding total DM. This mixture, compared with the only one without Giga that displayed a near‐sufficient clover proportion (Espanso with AberExcel), exhibited a 21·7% higher total DM (P < 0·05) and had far fewer weeds.     

Effects of continuous sheep stocking and strategic rest periods on the sward characteristics of binary perennial grass/white clover associations

Five binary perennial grass/white clover (Trifolium repens, cv. Menna) mixtures were evaluated over a 3-year period under continuous sheep stocking together with the imposition of a rest period for either an early or a late conservation cut; the experiment with plot sizes of 0·16 ha was replicated three times. The grass species and cultivars used were Merlinda tetraploid and Magella diploid perennial ryegrass (Lolium perenne), Prairial cocksfoot (Dactylis glomerata), Rossa meadow fescue (Festuca pratensis) and Goliath timothy (Phleum pratense). The greatest total lengths of white clover stolon developed in the meadow fescue (171·6 m m^?2) and timothy (151·9 m m^?2) associations compared with those in tetraploid perennial ryegrass (98·6 m m^?2), diploid perennial ryegrass (91·9 m m^?2) and cocksfoot (74·6 m m^?2) (s.e.d. 16·4, P < 0·001). On average, the proportion of white clover stolon that was buried was between 0·86 and 0·89 and this was more abundant in late than early season. Whereas timothy persisted, the persistence of meadow fescue was low under any of the managements tested and this was markedly reduced by the third grazing season. In the diploid perennial ryegrass sward, a late June to early August rest period for conservation enhanced white clover stolon length. An early April to late May rest period greatly reduced total white clover stolon length in both diploid perennial ryegrass and tetraploid perennial ryegrass associations (diploid perennial ryegrass-unrested 89 m m^?2, early rest 56·1 m m^?2, late rest 130·7 m m^?2; tetraploid perennial ryegrass - unrested 125·1 m m^?2, early rest 71 m m^?2, late rest 99·7 m m^?2; s.e.d. 19·19, P < 0·001). The numbers of white clover stolon growing points per unit stolon length were greatest when the sward was rested during late June to early August ?55·9 m^?1 stolon length compared with 45·7 m^?1 for an April to late May rest and 46 m^?1 in the absence of a rest (s.e.d. 2·59, P < 0·001). Likewise, the percentage of stolon above ground was greatest with the late June to early August rest ?15·78% compared with 10·61% for the April to late May rest and 7·69% for no rest (s.e.d. 1·569, P < 0·001). The complementary percentages of buried stolon indicate the important role this fraction has and the need to study stolon behaviour in grazing studies generally. It is concluded that, in relation to perennial ryegrass as a companion grass, meadow fescue and timothy allow better white clover development and cocksfoot less. However, other attributes have to be considered, for example the poor persistence of meadow fescue and the slower regrowth of timothy, both of which allow the invasion of weed grasses, or the lower acceptability of cocksfoot to livestock. The timing of the rest period before the conservation cut can influence white clover development considerably, but the effects differed with different companion grasses.     

Species interactions in a grassland mixture under low nitrogen fertilization and two cutting frequencies: 1. dry‐matter yield and dynamics of species composition

Four‐species mixtures and pure stands of perennial ryegrass, tall fescue, white clover and red clover were grown in three‐cut and five‐cut systems at Ås, southern Norway, at a low fertilization rate (100 kg N ha^?1 year^?1). Over a three‐year experiment, we found strong positive effects of species diversity on annual dry‐matter yield and yield stability under both cutting frequencies. The overyielding in mixtures relative to pure stands was highest in the five‐cut system and in the second year. Among the possible pairwise species interaction effects contributing to the diversity effect, the grass–grass interaction was the strongest, being significant in both cutting systems and in all years. The grass–legume interactions were sometimes significant, but no significant legume–legume interaction could be detected. Competitive relationships between species varied from year to year and also between cutting systems. Estimations based on species identity effects and pair‐specific interactions suggested that the optimal proportions of red clover, white clover, perennial ryegrass and tall fescue in seed mixtures would have been around 0·1, 0·2, 0·4 and 0·3 in the three‐cut system, and 0·1, 0·3, 0·3 and 0·3 in the five‐cut system.     

Effect of application timing and grass height on the nitrogen fertilizer replacement value of cattle slurry applied with a trailing‐shoe application system

This study investigated the effect of using a trailing‐shoe system to apply cattle slurry, under different conditions of grass height (low [LG]: freshly cut sward [4–5 cm height] vs. high [HG]: application delayed by 7–19 d and applied to taller grass sward [4–11 cm] height) and month of application (June vs. April), on the nitrogen fertilizer replacement value (NFRV) and apparent N recovery (ANR_S) of cattle slurry applied to grassland. NFRV was calculated using two methods: (i) NFRV_N based on the apparent recovery of slurry‐N relative to that of mineral‐N fertilizer; and (ii) NFRV_DM based on DM yield. The effect of applying slurry into HG swards, relative to LG swards, decreased the DM yield by 0·47 t ha^?1 (P ≤ 0·001), N uptake by 5 kg ha^?1 (P = 0·05), ANR_S by 0·05 kg kg^?1 (P = 0·036), NFRV_N by 0·05 kg kg^?1 (P = 0·090) and NFRV_DM by 0·11 kg kg^?1 (P < 0·001). It was concluded that the main factor causing these decreases with HG, compared with LG applications, was wheel damage affecting subsequent N uptake and growth of the taller grass sward.     

Herbage productivity of prairie grass, reed canary-grass and phalaris

The herbage production and quality of swards of three grass species, prairie grass (Bromus willdenowii Kunth), reed canary-grass (Phalaris arundinacea L.) and phalaris (Phalaris tuberosa L.) were compared with perennial ryegrass (Lolium perenne L.) and hybrid ryegrass (L. perenne L. ×L. muitiflorum Lam.) under 6–cut (experiment 1) and 4–cut (experiment 2) regimes over 3 years at Ayr; annually, 360 kg ha^-1 fertilizer N were applied. At Edinburgh prairie grass was compared with Italian ryegrass (L. multiflorum Lam.), timothy (Phleum pratense L.) and cocksfoot (Dactylis glomerata L.) under an annual 4–cut regime for 3 years (experiment 3); fertilizer N application totalled 350 kg ha^-1 annually. Prairie grass gave the highest annual dry matter (DM) production at Ayr, averaging 11·99 t ha^-1 in experiment 1 and 15·62 t ha^-1 in experiment 2. Reed canary-grass was much less productive whilst phalaris did not persist after harvest year 1. On average, prairie grass gave 8–10% more DM than the three ryegrasses in the 6-cut system but its advantage was much less under the 4-cut regime. In experiment 3, the DM production of prairie grass and Italian ryegrass were similar in year 1, but following winter damage prairie grass gave the lowest production in subsequent harvest years. Prairie grass had digestibility (OMD) values lower than the ryegrass but higher than reed canary-grass, timothy and cocksfoot. The water soluble carbohydrate (WSC) concentrations in prairie grass were markedly higher than in timothy and cocksfoot but lower than those in Italian ryegrass. Prairie grass had relatively low P and Mg concentrations. Reed canary-grass had relatively low OMD and Ca, but high N, P, K and Mg contents. It is concluded that prairie grass may have potential in the UK as a special-purpose species for conservation management but mainly in the milder climatic areas. The Phalaris species evaluated had disappointing agronomic potential.