Tolerance to frost and ice encasement in cultivars of timothy and perennial ryegrass during winter

Ice encasement causes major winter damage in grasslands in coastal areas of Northern Scandinavia and may also affect grass performance in a future changing climate. Changes in ice‐encasement tolerance (LD50), frost tolerance (LT50) and water‐soluble carbohydrate (WSC) content were investigated in different cultivars of timothy and perennial ryegrass sampled at three sites with contrasting conditions. Timothy endured ice encasement for 40 d more than ryegrass (maximum LD50 63 vs. 20 d), and a cultivar originating from 69°N tolerated significantly longer periods in ice than a cultivar from 59°N. A similar relationship between cultivar origin and tolerance was observed for ryegrass. The higher LD50 in timothy compared with ryegrass seemed to be associated with a lower rate of change in WSC content during ice encasement, but no apparent relationship was found between WSC content at the start of encasement and plant survival in ice. A significant linear relationship was found between LD50 and LT50 of plants sampled in the field. A differing decline in frost tolerance during ice encasement for the species indicated that timothy is more resistant to dehardening under ice than ryegrass. This study contributes data and functional relationships needed to develop models of grass performance during winter.     

Impact of frost and plant age on compensatory growth in timothy and perennial ryegrass during winter

Two cultivars of each of two contrasting grass species, timothy ( Phleum pratense L.) and perennial ryegrass ( Lolium perenne L.), sown either in May or July, were sampled for dry matter (DM) weight of roots and herbage in late October (end of main growing season) and examined for regrowth when exposed to a frost treatment (−8 to −10°C in darkness for 48 h) in late January, early March and late April. The frost treatment caused compensatory root and herbage growth, and regrowth of roots in March and April was greater than regrowth of herbage. Early-sown plants had higher DM weights of roots and herbage than late-sown plants in October but had the lowest survival rate when exposed to the frost treatment in April. Compensatory growth of root and herbage in response to moderate frosts occurred in January and March in all cultivars, and in the most frost-resistant timothy cultivar in April. Concentration of total carbohydrates increased from October to March with the greatest increase in the most winter-hardy cultivar of each species, and then decreased in April. An indirect correlation between concentration of total carbohydrates and tolerance to moderate frost was indicated, and plant survival and ability for compensatory growth were also associated with differences in adaptation related to winter survival in the cultivars tested.     

Component yields and quality of binary mixtures of lucerne and perennial, Italian or short rotation hybrid ryegrass

Studies were conducted to characterize sward dynamics of lucerne ( Medicago sativa L.) and perennial, Italian or short rotation hybrid rye-grass ( Lolium perenne L. and L. multiflorum Lam.) binary mixtures under hay management. Lucerne/timothy ( Phleum pratense L.) mixtures were included as controls. The study was conducted on two farms in central Pennsylvania, USA on Hagerstown silt loams (fine, mixed, mesic hapludalf). Total dry matter yield, grass yield and yield of digestible dry matter were higher for tetraploid perennial and tetraploid Italian ryegrass mixtures than for timothy mixtures. Seasonal production differed for the three kinds of ryegrass. Italian ryegrass dry matter yields were higher in cuts 2 and 3 than perennial ryegrass yields. Grass yields represented 34%, 24% and 12% of total yields of the Italian, perennial or short rotation, and timothy mixtures, respectively. However, herbage in vitro dry matter digestibility of Italian was lower than that of perennial ryegrass.
The tetraploid perennial ryegrasses Grimalda and Reveille had high-ranking N concentrations more frequently than other cultivars, while Bar-vestra and Reveille tetraploid perennials and Bison short rotation ryegrass had high-ranking dry matter digestibility values more frequently than the other cultivars. Perennial ryegrass cultivars and Lipo tetraploid Italian ryegrass persisted well for 4 years and produced higher yields with a lower weed content than timothy mixtures. It is concluded that perennial ryegrasses and Lipo Italian ryegrass should be recommended over timothy, as companion grasses with lucerne.     

Festulolium fibre characteristics and digestibility as affected by maturity

This study compared the forage quality of Festulolium entries of festucoid (Hykor) and loloid attributes (Felopa and Norwegian candivars) with major cultivars of the parent species perennial ryegrass and meadow fescue, and with timothy. Forage NDF, iNDF, kdNDF and DDM were analysed at vegetative stage, early heading, heading and anthesis during spring growth (SG) and two subsequent regrowths over two production years at two locations in Norway. NDF and iNDF increased and kdNDF and DDM decreased with advancing maturity, mostly expressed in SG, with negative correlations between DDM and NDF and iNDF and ADL, and strong positive correlations with kdNDF. In SG, across years and locations, the content of NDF at heading stage was 30% higher and iNDF 400% higher in stems than in leaves, while stem kdNDF was about 50% of that in leaves. In general, timothy cv. Grindstad and Festulolium cv. Hykor had the highest NDF and iNDF and the lowest kdNDF values. Loloid entries of Festulolium were quite similar to perennial ryegrass, although the tetraploid entry proved better than the others with respect to forage quality. Regarding nutritive value, the festucoid and loloid types of Festulolium should be considered separate groups. The effect of year was more pronounced than that of location, for which the ranking of entries was quite similar. Loloid Festulolium is prone to winter damage, and, although it has higher nutritive value than the parent species, its use will depend on the conditions for winter survival.     

Effects of white clover cultivar and companion grass on winter survival of seedlings in autumn-sown swards

The aim was to study the effects of white clover cultivar and combinations with perennial ryegrass cultivars on seedling establishment in autumn‐sown swards and on winter survival of seedlings. Large‐leaved white clover cv. Alice and small‐leaved white clover cv. Gwenda, and an erect and a prostrate perennial ryegrass cultivar were sown in autumn in pure stands and as four binary grass‐clover mixtures. Mixtures of white clover cv. Huia and Aberherald with perennial ryegrass were also sown. Companion grasses had no significant impact on the establishment of white clover. The number of seedlings of white clover cv. Alice in mixtures (335 m^?2) was higher than cv. Gwenda (183 m^?2) and pure swards had similar white clover population densities as mixed swards. White clover cv. Huia tended to have more seedlings than Aberherald (355 and 205 m^?2 respectively). No stolons were produced prior to a severe winter, because of the late sowing date. Winter survival of clover seedlings was 0·56 in mixtures and 0·69 in pure stands, irrespective of white clover or companion grass cultivar. Stolon development of white clover in autumn is often considered essential for overwintering survival and spring growth. In this study, there was considerable survival of the non‐stoloniferous tap‐rooted seedlings of all four clover cultivars despite a severe winter.     

Influence of herbage species, cultivar and cutting date on fatty acid composition of herbage and lipid metabolism during ensiling

The fatty acid (FA) concentration of herbage and lipid metabolism in silage, mainly oxidation and lipolysis, of different species (perennial ryegrass, red clover and white clover) and three cultivars of white and red clover at three cutting dates in the growing season (April, July and October) were studied. FA concentration and composition was strongly affected by species and cutting date. Perennial ryegrass had lower concentrations of C16:1, C18:0, C18:1 and C18:2 than red and white clover. Within red and white clover, the effect of cultivar was small. Oxidation of C18:3 during wilting was different between species and cutting date despite similar wilting conditions. Lipolysis in silage was also influenced by cutting date, species and to some extent by cultivar. Furthermore, in some cuts silages of red and white clover displayed a lower lipolysis than silage of perennial ryegrass. On average, over the three cutting dates proportionately 0·903, 0·864 and 0·857 of the membrane lipids in perennial ryegrass, red clover and white clover were hydrolysed during ensiling. In red clover this could be due to the lipid-protecting properties of polyphenol oxidase (PPO) activity. This was not observed in perennial ryegrass or white clover. Nevertheless, differences in lipolysis in silage between cultivars of red clover were not correlated with PPO activity.     

THE EFFECT OF DEFOLIATION ON THE SEED-PRODUCING CAPACITY OF BRED STRAINS OF GRASSES

A leafy strain of timothy and of perennial ryegrass showed no significant reductions in seed yields following grazing at different dates from October to January, when extra nitrogen was applied.
Spring and winter + spring grazing, together with extra nitrogen, significantly reduced the seed yield of S50 timothy for three successive harvest years.
Perennial ryegrass S101 showed no reduction after spring or winter + spring grazing when defoliation was made not later than mid-April, but when grazing was delayed until May and drought occurred there was a significant reduction in seed yield.     

A COMPARISON OF BROADCAST AND WIDE-ROW SPACED GRASSES WHEN MANAGED FOR FOGGAGE PRODUCTION

A small-plot trial comparing perennial ryegrass, cocksfoot, timothy and meadow fescue sown broadcast and also in 21 inch rows was conducted at Auchincruive from 1954 to 1957. The cutting treatments were arranged so that measurement could be made of both summer and winter production.
Of the four species, cocksfoot was the most suitable for foggage production. Perennial ryegrass suffered severely from winter killing. Timothy and meadow fescue, although persistent, did not yield as much as cocksfoot.
Total dry-matter production over three years was higher from the cocksfoot rows than from the broadcast stand. For ryegrass and meadow fescue, broadcasting gave higher yields than row sowing while timothy showed no significant difference between sowing methods.
During the winter, all species showed a loss of dry matter. Losses for ryegrass, cocksfoot, timothy and meadow fescue were respectively 34%, 17%, 18% and 24%. Broadcast stands averaged 11% greater loss than the rows.     

Morphology and nutritive value of perennial ryegrass cultivars at different phenological stages

Eight perennial ryegrass cultivars (representing the variations in ploidy, heading date and water‐soluble carbohydrates concentration) were investigated for morphology and nutritive value at three phenological stages: pre‐heading vegetative, reproductive and post‐flowering vegetative stages. Chemical compositions and digestibility of morphological components (lamina, pseudostem and reproductive stem) from each perennial ryegrass cultivar were analysed in a split‐plot design. At the vegetative stages, perennial ryegrass cultivars differed significantly in the proportions of lamina and pseudostem. Tetraploid cultivars, Base and Bealey, always had the greatest lamina proportion (51.8% and 53.2% at the pre‐heading and post‐flowering vegetative stages respectively). At the reproductive stage, the emergence of seed heads diminished the differences in morphology among cultivars. Perennial ryegrass cultivars also had distinct nutritive value throughout three phenological stages. The high‐sugar cultivar, AberMagic, had high WSC concentrations (276, 227 and 90 g/kg DM at the pre‐heading vegetative, reproductive and post‐flowering vegetative stages respectively); the intermediate‐heading cultivars, Kamo and Commando, generally had a lower organic matter digestibility in dry matter than the late‐heading cultivars at the pre‐heading vegetative (70.7% vs. 74.4%) and reproductive stage (63.9% vs. 68.2%). However, although the morphological components were different in nutritive value consistently, the differences in morphology did not account for the variation in nutritive value among perennial ryegrass cultivars.     

Variation in spring temperatures, grass production and response to nitrogen over twenty years in the uplands

Large between-year variation was observed in spring air and soil temperatures at Pant-y-dwr Hill Centre (305 m altitude) from 1967 to 1984 and at Bronydd Mawr Research Centre (330 m) from 1985 to 1986 in Powys. The mean date of attaining T-sum 200°C accumulated air temperature was 13 March (range 9 February to 23 April) and that of soil temperature at 100 mm depth permanently above 5 5°C was 9 April (range 7 March to 4 May). Net herbage accumulation and response to applied N from Aberystwyth S23 perennial ryegrass (Lolium perenne L.) swards during April were also highly variable and were correlated with the date of reaching target soil temperatures of above 5 5°C for 5 consecutive days ( r =−0·68, P < 0·001 for net herbage accumulation and r =−0·70, P < 0·001 for response to N).
The use of early flowering varieties of perennial ryegrass gave a significant increase of herbage growth in spring compared with late varieties. From 1979 to 1980 net herbage accumulation during April from the early variety Frances was 94% more than from Perma (late) and 55% more than from Talbot (intermediate) varieties. Under conservation management more winter hardy and persistent varieties of Italian and hybrid ryegrasses ( L. multiflorum L. and L. multiflorum X L. perenne ) gave higher quantities of firstcut silage crops in early June than RvP Italian ryegrass.
After the severe winters of 1978–79 and 1985–86, subsequent spring production from a wide range of ryegrasses was shown to be affected by sward survival, highlighting the value of winter hardy varieties when resowing in the uplands     

THE EFFECT OF AUTUMN MANAGEMENT ON WINTER DAMAGE AND SUBSEQUENT SPRING PRODUCTION OF SIX VARIETIES OF LOLIUM PERENNE GROWN AT CAMBRIDGE

Six perennial ryegrass varieties which were known to differ in winter hardiness were subjected to ten managements comprising two N rates and fire autumn cutting treatments. Autumn yields and soluble carbohydrate contents were measured, also the amount of winter damage in February and subsequent spring yields. The winter was mild, herbage damage was slight and no plant deaths were recorded, but significant differences were found between cutting treatments and varieties. The amount of winter damage increased with the length of the autumn rest period and with the degree of winter dormancy of individual varieties, indicating that natural senescence was a more important causative agent than frost action. Spring yields were affected by the amount of winter damage but there were considerable interactions between varieties and environments. The results are discussed in relation to autumn management practices, choice of variety for different locations and measurements of winter hardiness. The induction of winter dormancy by day length or low temperature reactions is considered in the breeding of varieties for maximum production in different parts of the country.     

The silage yield potential of hybrid ryegrass over a 5-year period in Northern Ireland relative to other ryegrasses and timothy

The yield and persistency of grass species within a managed sward are a major consideration when determining species mixtures for either long-term or short-term sward management systems. Perennial ryegrass is lower yielding but more persistent than Italian ryegrass. Hybrid ryegrass, an interspecies cross between perennial and Italian ryegrass, may be higher yielding than perennial ryegrass and more persistent than Italian ryegrass. Therefore, the yield and persistency of hybrid ryegrass was investigated in Northern Ireland in five experiments, each harvested over five consecutive years, and compared with that of Italian ryegrass, perennial ryegrass and timothy. The performance of the five grass species were in the expected order; Italian ryegrass was higher yielding than hybrid ryegrass, which was higher than perennial ryegrass, with timothy the lowest, whereas perennial ryegrass and timothy had significantly higher sward densities than the hybrid and Italian ryegrasses. In addition, it was found that the rate of decline in yield and sward density with age was the same for all species. Consequently, this study indicated that the higher yielding Italian and hybrid ryegrasses could continue to out-perform perennial ryegrass for 5 years, opening the possibility of more extensive farming use of these species, particularly in Northern Ireland.     

The value of timothy (Phleum pratense L.) in ryegrass — timothy mixtures managed to simulate intensive grazing

The performance of timothy in mixtures with perennial ryegrass was assessed under a simulated intensive grazing management over two harvest years in 1974–75. Three seed rates of S23 perennial ryegrass were factorially combined with three rates of Scots timothy and compared with pure stands of each grass. All were sown with Huia white clover. When cut six times at monthly intervals and with an annual N input of 350 kg ha^?1, there were no significant differences in total DM production in either year. The 2-year mean DM yield for the nine mixtures and six pure swards was 9·77 t ha^?1 (range 9·34–10·16). Compared with the pure ryegrass swards, in both years the ryegrass-timothy mixtures produced earlier spring growth but were significantly lower yielding at the second cut. Over the first five cuts the proptortion of timothy in the three mixtures with 22·4 kg ha^?1 ryegrass seed averaged 26% in the first year and 37% in the second. Corresponding calculated mean DM yields of timothy were 2·75 and 3·00 t ha^?1. It is concluded that an early timothy variety is capable of competing with a late-heading perennial ryegrass in frequently cut swards managed to simulate intensive grazing. The strong development of timothy in the dry summer of 1975 suggests that in mixtures of late perennial ryegrass varieties, an early variety of timothy should be beneficial for its spring growth in grazed swards.     

The rate of water loss from cut grass of different species dried at 20°C

The water loss from tall fescue cv. S170, perennial ryegrass cv. S24, Italian ryegrass cv. R v P and timothy cv. S352 when dried as a thin layer under controlled conditions at 20°C and r.h. 50% is described. Comparisons were made on six occasions between mid-April and mid-June. Tall fescue dried faster than the other species and the time to reach a water concentration of 0·2 g water per g dry matter varied little between the six harvests. In contrast, the drying time for the ryegrasses and timothy increased to maximal values in mid-May and then fell. Drying time was dependent on the initial water concentration of the grass, the ratio of leaf to stem and on the amount of true stem exposed to the drying environment. The practical implications in relation to haymaking are discussed.     

SEASONAL TILLER POPULATIONS OF GRASS AND GRASSJCLOVER SWARDS WITH AND WITHOUT IRRIGATION

The number of tillers per unit area of pure swards of S23 and S24 perennial ryegrass, S37 cocksfoot, S48 timothy and of a S23 perennial ryegrass/white clover sward, were examined at intervals of 4 weeks during a period of two years. The monthly appearance of new tillers and the death of tillers of S23 and S24 ryegrass and S48 timothy were followed for 15 months. The studies were made with and without irrigation.
The number of tillers varied with season through the year. Tiller numbers increased rapidly in late winter or early spring, and then declined until midsummer. Numbers rose again between June and August. This seasonal pattern of tiller numbers was most marked in ryegrass and timothy and least in cocksfoot. The effect of irrigation on tiller numbers was variable, and usually small in relation to the overall seasonal fluctuations in tiller numbers, but dry soil conditions could delay an increase in tiller numbers between June and August. The relationship between seasonal fluctuations in tiller number and root growth of swards is discussed.     

Effect of temperature regime on germination of seed of perennial ryegrass (Lolium perenne)

Perennial ryegrass ( Lolium perenne L.) is one of the most valuable temperate pasture grasses in the world. Understanding seed germination in response to temperature can help determine its optimal seeding times. Germination tests for two cultivars of perennial ryegrass (cv. 'Turfstar' and cv. 'Transtar') were conducted by applying thirty-six constant and alternating temperature regimes, ranging from 5 to 40°C (16 h night/8 h day). Responses in germination rate to these temperature regimes were then used to construct a quadratic response surface, giving estimated germination rates with confidence intervals at P  ≤   0·05 for both cultivars. Optimum germination rate occurred over a range of regimes: 15/25, 20/25, 20/30 and 25/30°C. Furthermore, a wide range in diurnal alternating temperatures resulted in maximum seed germination rate for cv. 'Transtar', while no constant temperature regime supported maximum germination rate for cv. 'Turfstar'. A temperature-dependent germination rate function for perennial ryegrass was developed which could be used in the future to predict location-specific optimum seeding times when national or regional monthly temperature data are available.     

GRASSES IN WINTER

The Aberystwyth district experienced exceptionally cold winter weather in 1954, 1955 and 1956, especially during the period from the end of January to early March. Its effect on the grasses, both in the single-plant nurseries and in the agronomic experimental areas, is briefly described.
It was observed that plants which entered the winter carrying a bulk of herbage in a winter-proud condition were at a disadvantage compared with those which had been reasonably grazed or cut back during the autumn. The younger plants were found to be better able than the older ones to withstand extreme conditions.
Suggestions are made as to how some simple-mixture swards can be managed so as to be reasonably likely to provide keep in situ during the difficult months December to April. The species under consideration are cocksfoot ( Dactylis glomerata ), timothy ( Phleum pratense and P. nodosum ), tall fescue ( Festuca arundinacea ), perennial ryegrass ( Lolium perernne ) and Italian ryegrass ( L. italicum ).     

The ingress of Poa annua into perennial ryegrass swards

The ingress of Poa annua into established swards of perennial ryegrass was recorded in four experiments aimed at determining whether ingress was due to P. annua aggression or to a decrease in ryegrass vigour. Emphasis was placed on severity of defoliation, effect of bare spaces and the time of year most favourable to colonization.
In Experiment 1, P. annua ingress was greatest in the autumn, especially where ryegrass had been sown in rows rather than broadcast sown. In Experiment 2, seedlings of P. annua only established successfully in mature ryegrass swards that had been defoliated at intervals of two weeks. Experiment 3 showed that ingress was inversely related to the vigour of the ryegrass cultivar, being particularly high on frequently defoliated plots. In Experiment 4, which involved a study of the colonization of poached areas, more seedlings established in quadrats surrounded by P. annua -dominant pasture compared with patches surrounded by perennial ryegrass.
It is concluded that P. annua is not an aggressive species; it colonizes only open ryegrass swards that have become weakened by intensive management.     

Fatty acid composition and nutritive value of twelve cultivars of perennial ryegrass

A comparison was made of the fatty acid composition and nutritive value of twelve cultivars of perennial ryegrass ( Lolium perenne L.) differing in heading date and ploidy level. The cultivars were sown in triplicate plots and three sequential cuts of herbage were taken at 20-d intervals during the late spring and early summer to describe the fatty acid composition and other measurements of nutritive value. Differences between cultivars were recorded for DM content ( P  <   0·01) and concentrations of gross energy, neutral-detergent fibre (NDF) and ash ( P  <   0·05). Diploid cultivars had a higher DM content and concentration of NDF ( P  <   0·01) than tetraploid cultivars whilst late-heading date cultivars had the lower NDF ( P  <   0·05), ADF and ash ( P  <   0·01) concentrations. There was variation between cultivars in fatty acid composition. Diploid cultivars had a higher concentration of C18:0 ( P  <   0·01) and C18:1 ( P  <   0·05) than tetraploid cultivars and late-heading date cultivars had the highest concentrations of total fatty acids, C16:0, C18:2 and C18:3 ( P  <   0·05). This was predominantly due to the cultivar Tyrella which is a diploid, late-heading cultivar. The study showed that some variation exists between perennial ryegrass cultivars in concentrations of polyunsaturated fatty acids, which may present opportunities to select for this trait, but further research on the developmental stages and degree of leafiness of cultivars is first required.     

THE EFFECT OF METHOD OF SOWING ON THE ESTABLISHMENT OF GRASS SEED CROPS

Methods used by growers to establish seed crops of perennial ryegrass, cocksfoot, timothy and meadow fescue have been examined. While satisfactory establishments were obtained by sowing perennial ryegrass broadcast, cocksfoot, timothy and meadow fescue should be sown in rows, especially if they are to be sown under a cover crop.